CN107254412B - 一种用于修复羟甲基纤维素废水的微生物制剂及其制备方法 - Google Patents
一种用于修复羟甲基纤维素废水的微生物制剂及其制备方法 Download PDFInfo
- Publication number
- CN107254412B CN107254412B CN201710544293.3A CN201710544293A CN107254412B CN 107254412 B CN107254412 B CN 107254412B CN 201710544293 A CN201710544293 A CN 201710544293A CN 107254412 B CN107254412 B CN 107254412B
- Authority
- CN
- China
- Prior art keywords
- domestication
- solution
- wastewater
- culture medium
- aspergillus niger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002351 wastewater Substances 0.000 title claims abstract description 55
- 229920003063 hydroxymethyl cellulose Polymers 0.000 title claims abstract description 29
- 229940031574 hydroxymethyl cellulose Drugs 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 230000000813 microbial effect Effects 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 34
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 32
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 32
- 241000186220 Cellulomonas flavigena Species 0.000 claims abstract description 32
- 241000192542 Anabaena Species 0.000 claims abstract description 21
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims description 31
- 238000012258 culturing Methods 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 22
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 20
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 16
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- 241000195493 Cryptophyta Species 0.000 claims description 12
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 12
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 12
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 238000007796 conventional method Methods 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 241000078013 Trichormus variabilis Species 0.000 claims description 8
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 8
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 8
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 8
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 8
- 239000011790 ferrous sulphate Substances 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 8
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 4
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 claims description 4
- 239000004202 carbamide Substances 0.000 claims description 4
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 4
- 229960004642 ferric ammonium citrate Drugs 0.000 claims description 4
- 239000004313 iron ammonium citrate Substances 0.000 claims description 4
- 235000000011 iron ammonium citrate Nutrition 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 4
- 235000011164 potassium chloride Nutrition 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 3
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims 1
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims 1
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims 1
- 229960003943 hypromellose Drugs 0.000 claims 1
- 238000005067 remediation Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 11
- 108090000790 Enzymes Proteins 0.000 abstract description 4
- 102000004190 Enzymes Human genes 0.000 abstract description 4
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- 230000035755 proliferation Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 51
- 108010059892 Cellulase Proteins 0.000 description 9
- 229940106157 cellulase Drugs 0.000 description 9
- 229920002678 cellulose Polymers 0.000 description 9
- 239000001913 cellulose Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 239000001768 carboxy methyl cellulose Substances 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 5
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 4
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 4
- 238000005868 electrolysis reaction Methods 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 235000003239 Guizotia abyssinica Nutrition 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- -1 carbohydrate compound Chemical class 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 2
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical group [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229920006321 anionic cellulose Polymers 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 229920003086 cellulose ether Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 description 1
- 229940106681 chloroacetic acid Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229960004756 ethanol Drugs 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000008394 flocculating agent Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000004872 foam stabilizing agent Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- QMQXDJATSGGYDR-UHFFFAOYSA-N methylidyneiron Chemical compound [C].[Fe] QMQXDJATSGGYDR-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- QDRKDTQENPPHOJ-UHFFFAOYSA-N sodium ethoxide Chemical compound [Na+].CC[O-] QDRKDTQENPPHOJ-UHFFFAOYSA-N 0.000 description 1
- 229940023144 sodium glycolate Drugs 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- JEJAMASKDTUEBZ-UHFFFAOYSA-N tris(1,1,3-tribromo-2,2-dimethylpropyl) phosphate Chemical compound BrCC(C)(C)C(Br)(Br)OP(=O)(OC(Br)(Br)C(C)(C)CBr)OC(Br)(Br)C(C)(C)CBr JEJAMASKDTUEBZ-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明属于微生物技术领域，公开了一种用于修复羟甲基纤维素废水的微生物制剂，其由如下原料组成：多变鱼腥藻驯化液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液、黑曲霉驯化液；所述多变鱼腥藻液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液以及黑曲霉驯化液分开使用。本发明微生物制剂采用四种微生物，配伍合理，协同性能好，能够促进微生物增殖以及酶活力，修复羟甲基纤维素废水效果好。
Description
技术领域
本发明属于微生物技术领域，具体涉及一种用于修复羟甲基纤维素废水的微生物制剂及其制备方法。
背景技术
羧甲基纤维素(CMC)是一种纤维素衍生物，属阴离子型纤维素醚类，外观为白色或微黄色絮状纤维粉末或白色粉末，无臭无味，无毒；易溶于冷水或热水，形成具有一定粘度的透明溶液。溶液为中性或微碱性，不溶于乙醇、乙醚、异丙醇、丙酮等有机溶剂，可溶于含水60%的乙醇或丙酮溶液。有吸湿性，对光热稳定，粘度随温度升高而降低，溶液在PH值2～10稳定，PH低于2，有固体析出，PH值高于10粘度降低。变色温度227℃，炭化温度252℃，2%水溶液表面张力71mn/n。CMC可作为粘合剂 、增稠剂、悬浮剂、乳化剂、分散剂、稳定剂、上浆剂等。羧甲基纤维素钠（CMC）是纤维素醚类中产量最大的、用途最广、使用最为方便的产品，俗称为"工业味精"。在食品工业中被用作增稠剂及乳化剂,在日化工业中被用作稳泡剂及分散剂，在医药工业中被用作塑性剂及缓释剂等，在石油工业中被用作保水剂和稳定剂等。CMC 生产废水是典型的高浓度有机废水，既含有纤维素、羧甲基纤维素钠等大分子物质，又含有羟乙酸钠、氯乙酸、乙醇、乙醇钠以及无机盐等小分子物质，COD、氨氮含量较高，为难处理高浓度工业废水。若未经处理直接排放将会直接导致江河水质矿化度提高,给土壤、地表水、地下水带来严重的污染，使水体缺氧甚至是厌氧，导致鱼类及其他生物大量死亡。
目前处理羟甲基纤维素废水的方法有较多，包括微电解法、紫外线氯化法、双氧水处理法、生物技术法等，但是均不能够实现废水有效利用，变废为宝。例如，现有技术“铁炭微电解法预处理羧甲基纤维素生产废水“采用微电解法处理生产废水，能够大幅降低废水中的污染物含量，但是经过处理后的废水仍然达不到排放标准，无法实现对废水进行资源化处理。生化技术法环境友好性好，能够对废水进行深度修复，缺点效率较低，菌株选择困难。开发一种用于修复羟甲基纤维素废水的高效微生物制剂是我们需要解决的技术问题。
发明内容
为了克服现有技术微生物种类选择困难、处理效果差的缺陷，本发明提供了一种用于修复羟甲基纤维素废水的微生物制剂。本发明还提供了上述微生物制剂的制备方法。
本发明是采用如下技术方案实现的：
一种用于修复羟甲基纤维素废水的微生物制剂，其由如下原料组成：多变鱼腥藻驯化液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液、黑曲霉驯化液；所述多变鱼腥藻液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液以及黑曲霉驯化液分开使用。
进一步地，所述多变鱼腥藻驯化液按照如下工艺制备而得：
1）取羟甲基纤维素废水，往废水中添加尿素0.5g、碳酸氢钠6g、磷酸氢二钾0.2g、磷酸二氢钾0.2g、氯化钠0.3g、氯化钾0.3g、柠檬酸铁铵0.03g、乙二胺四乙酸二钠0.03g，搅拌均匀，定容至1L，制得藻类驯化培养基；
2）将多变鱼腥藻按照常规培养获得种子液，然后按照1:10的体积比转入藻类驯化培养基，培养12小时，得到多变鱼腥藻驯化液。
进一步地，所述产黄纤维单胞菌驯化液、按照如下工艺制备而得：
1）取羟甲基纤维素废水，往废水中添加葡萄糖30-40g，酵母粉15-20g，磷酸二氢钾2-3g，磷酸氢二钾2-3g，硫酸镁2-3g，碳酸钙1-2g，硫酸亚铁1-2g，搅拌均匀，定容至1L，制得菌株驯化培养基；
2）将产黄纤维单胞菌按照常规培养获得种子液，再按照1:10的体积比转入菌株驯化培养基，培养12-16小时，得到产黄纤维单胞菌驯化培养液。
进一步地，所述枯草芽孢杆菌驯化液按照如下工艺制备而得：
1) 取羟甲基纤维素废水，往废水中添加葡萄糖30-40g，酵母粉15-20g，磷酸二氢钾2-3g，磷酸氢二钾2-3g，硫酸镁2-3g，碳酸钙1-2g，硫酸亚铁1-2g，搅拌均匀，定容至1L，制得菌株驯化培养基；
2)将枯草芽孢杆菌按照常规培养获得种子液，再按照1:15的体积比转入菌株驯化培养基，培养18-24小时，得到枯草芽孢杆菌驯化培养液.
进一步地，所述黑曲霉驯化液按照如下工艺制备而得：
1) 取羟甲基纤维素废水，往废水中添加葡萄糖30-40g，酵母粉15-20g，磷酸二氢钾2-3g，磷酸氢二钾2-3g，硫酸镁2-3g，碳酸钙1-2g，硫酸亚铁1-2g，搅拌均匀，定容至1L，制得菌株驯化培养基；
2)将黑曲霉按照常规培养获得种子液，再按照1:10的体积比转入菌株驯化培养基，培养18-24小时，得到黑曲霉驯化培养液。
本发明还要求保护上述任一权利要求所述的微生物制剂的制备方法及其在修复羟基甲纤维素废水中的应用。
本发明有益效果主要包括以下几个方面：
本发明采用四种微生物，配伍合理，协同性能好，能够促进微生物增殖以及酶活力，修复废水效果好；
产黄纤维单胞菌能够利用纤维素类物质作为养料，产生糖类物质作为黑曲霉的养料；枯草芽孢杆菌产蛋白酶、糖苷酶、淀粉酶、纤维素酶等，可以利用纤维素类物质以及无机氮作为养料；黑曲霉不能利用纤维素作为养料，但是能有效快速利用糖类物质以及无机物。
本发明中，与单独培养枯草芽孢杆菌以及产黄纤维单胞菌相比较，与黑曲霉共培养后，纤维素酶活力明显增加；可能是因为枯草芽孢杆菌以及产黄纤维单胞菌分解纤维素类物质产生的糖类化合物可被黑曲霉快速利用，酶促底物含量的降低，会加速纤维素酶的合成；
本发明中，产黄纤维单胞菌、枯草芽孢杆菌以及黑曲霉均为好养微生物，废水中含氧量较低，接入多变鱼腥藻可以在光合作用下提供产生的氧气或代谢产物，以增加培养基中的含氧量或营养分成，能够提高菌类的活性或繁殖率；
本发明使用的微生物首先经过废液驯化培养，能够更快速适应废水，在获得微生物蛋白的同时净化了水质，一举两得。
附图说明
图1：本发明各微生物之间协同产纤维素酶试验。
具体实施方式
为了使本技术领域的人员更好地理解本申请中的技术方案，下面将结合本申请具体实施例，对本申请的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本申请一部分实施例，而不是全部的实施例。基于本申请中的实施例，本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例，都应当属于本发明保护的范围。
实施例1
一种用于修复羟甲基纤维素废水的微生物制剂，其由如下原料组成：多变鱼腥藻驯化液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液、黑曲霉驯化液；所述多变鱼腥藻液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液以及黑曲霉驯化液分开使用。
所述多变鱼腥藻驯化液按照如下工艺制备而得：取羟甲基纤维素废水，往废水中添加尿素0.5g、碳酸氢钠6g、磷酸氢二钾0.2g、磷酸二氢钾0.2g、氯化钠0.3g、氯化钾0.3g、柠檬酸铁铵0.03g、乙二胺四乙酸二钠0.03g，搅拌均匀，定容至1L，制得藻类驯化培养基；将多变鱼腥藻按照常规培养获得种子液（浓度控制在1×105个/ml），然后按照1:10的体积比转入藻类驯化培养基，培养12小时，得到多变鱼腥藻驯化液；
所述产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液以及黑曲霉驯化液按照如下工艺制备而得：
取羟甲基纤维素废水，往废水中添加葡萄糖40g，酵母粉20g，磷酸二氢钾3g，磷酸氢二钾3g，硫酸镁3g，碳酸钙2g，硫酸亚铁2g，搅拌均匀，定容至1L，制得菌株驯化培养基；
将产黄纤维单胞菌按照常规培养获得种子液（浓度控制在1×108个/ml），再按照1:10的体积比转入菌株驯化培养基，培养16小时，得到产黄纤维单胞菌驯化培养液；
将枯草芽孢杆菌按照常规培养获得种子液（浓度控制在1×108个/ml），再按照1:15的体积比转入菌株驯化培养基，培养24小时，得到枯草芽孢杆菌驯化培养液；
将黑曲霉种子液按照常规培养获得种子液（浓度控制在1×108个/ml），再按照1:10的体积比转入菌株驯化培养基，培养24小时，得到黑曲霉驯化培养液。
所述多变鱼腥藻为 ATCC 29413；所述产黄纤维单胞菌为ATCC 482，所述枯草芽孢杆菌为ATCC 6633，所述黑曲霉为ATCC 16404。
实施例2
一种用于修复羟甲基纤维素废水的微生物制剂，其由如下原料组成：多变鱼腥藻驯化液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液、黑曲霉驯化液；所述多变鱼腥藻液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液以及黑曲霉驯化液分开使用。
所述多变鱼腥藻驯化液按照如下工艺制备而得：取羟甲基纤维素废水，往废水中添加尿素0.5g、碳酸氢钠6g、磷酸氢二钾0.2g、磷酸二氢钾0.2g、氯化钠0.3g、氯化钾0.3g、柠檬酸铁铵0.03g、乙二胺四乙酸二钠0.03g，搅拌均匀，定容至1L，制得藻类驯化培养基；将多变鱼腥藻按照常规培养获得种子液（浓度控制在1×105个/ml），然后按照1:10的体积比转入藻类驯化培养基，培养12小时，得到多变鱼腥藻驯化液；
所述产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液以及黑曲霉驯化液按照如下工艺制备而得：
取羟甲基纤维素废水，往废水中添加葡萄糖30g，酵母粉15g，磷酸二氢钾2g，磷酸氢二钾2g，硫酸镁2g，碳酸钙1g，硫酸亚铁1g，搅拌均匀，定容至1L，制得菌株驯化培养基；
将产黄纤维单胞菌按照常规培养获得种子液（浓度控制在1×108个/ml），再按照1:10的体积比转入菌株驯化培养基，培养12小时，得到产黄纤维单胞菌驯化培养液；
将枯草芽孢杆菌按照常规培养获得种子液（浓度控制在1×108个/ml），再按照1:15的体积比转入菌株驯化培养基，培养18小时，得到枯草芽孢杆菌驯化培养液；
将黑曲霉种子液按照常规培养获得种子液（浓度控制在1×108个/ml），再按照1:10的体积比转入菌株驯化培养基，培养18小时，得到黑曲霉驯化培养液。
所述多变鱼腥藻为 ATCC 29413；所述产黄纤维单胞菌为ATCC 482，所述枯草芽孢杆菌为ATCC 6633，所述黑曲霉为ATCC 16404。
实施例3
本发明实施例1-2采用的羟甲基纤维素废水是经过预处理后的废水，预处理步骤为：羟甲基纤维素生产废水首先经过絮凝沉淀池，絮凝剂为硫酸铝，添加量为1-3wt%，然后采用微电解法进行处理，再进入酸解调节池，调整溶液的pH为6.5-7；经过预处理后的废水各项主要指标为：COD 1094 mg/L、BOD 613 mg/L、氨氮 215 mg/L、SS 41mg/L、总盐389mg/L、纤维素326mg/L、羟甲基纤维素592 mg/L。
以本发明实施例1为例，本发明微生物制剂的使用方法为：经过预处理的废水进入微生物反应池，按照4%的接种量接种多变鱼腥藻驯化液，培养12h后，再按照6%的接种量接种产黄纤维单胞菌驯化培养液、6%的接种量接种枯草芽孢杆菌驯化培养液，继续培养12h后，再按照8%的接种量接种黑曲霉驯化培养液，继续培养48h，过滤收集藻体和菌体，滤液排出。
对照组1：不添加藻类，其余同实施例1；对照组2：不添加黑曲霉，其余同实施例1；经过各组处理后排出的水的各项指标见表1：
表1
| 指标 | 实施例1 | 对照组1 | 对照组2 |
| COD mg/L | 10.8 | 106.9 | 138.9 |
| BOD mg/L | 8.9 | 89.5 | 71.4 |
| 氨氮 mg/L | 9.6 | 40.2 | 59.0 |
| SS mg/L | 2.3 | 7.7 | 10.5 |
| 总盐 mg/L | 24.7 | 81.4 | 84.1 |
| 纤维素 mg/L | 4.4 | 39.8 | 46.6 |
| 羟甲基纤维素 mg/L | 4.1 | 60.7 | 27.5 |
结论：经过微生物深度处理后，各污染物含量大大降低，符合排放标准；经过对比实验可知，各微生物之间能够共生，协同性能好。
实施例4
本发明各微生物之间协同产纤维素酶试验：
设置三个组别：
实验组：实施例1制备的微生物制剂，使用方法同实施例3；
对照组1：不添加藻类，其余同实验组；
对照组2：不添加黑曲霉，其余同实验组；
对照组3：不添加藻类和黑曲霉，其余同实验组。
培养结束后，纤维素酶的酶活力如图1所示，实验组的纤维素酶活力远高于对照组1-2，与对照组3相比，酶活力可提高50%以上。主要原因可能是，枯草芽孢杆菌以及产黄纤维单胞菌分解纤维素类物质产生的糖类化合物可被黑曲霉快速利用，酶促底物含量的降低，会加速纤维素酶的合成；多变鱼腥藻可以通过光合作用产生的氧气或代谢产物，以增加培养基中的含氧量或营养分成，能够提高菌类的活性或繁殖率。
尽管已经示出和描述了本发明的实施例，对于本领域的普通技术人员而言，可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型，本发明的范围由所附权利要求及其等同物限定。
Claims (2)
1.一种用于修复羟甲基纤维素废水的微生物制剂，其由如下原料组成：多变鱼腥藻驯化液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液、黑曲霉驯化液；所述多变鱼腥藻液、产黄纤维单胞菌驯化液、枯草芽孢杆菌驯化液以及黑曲霉驯化液分开使用；
所述多变鱼腥藻驯化液按照如下工艺制备而得：
1）取羟甲基纤维素废水，往废水中添加尿素0.5g、碳酸氢钠6g、磷酸氢二钾0.2g、磷酸二氢钾0.2g、氯化钠0.3g、氯化钾0.3g、柠檬酸铁铵0.03g、乙二胺四乙酸二钠0.03g，搅拌均匀，定容至1L，制得藻类驯化培养基；
2）将多变鱼腥藻按照常规培养获得种子液，然后按照1:10的体积比转入藻类驯化培养基，培养12小时，得到多变鱼腥藻驯化液；
所述产黄纤维单胞菌驯化液、按照如下工艺制备而得：
3）取羟甲基纤维素废水，往废水中添加葡萄糖30-40g，酵母粉15-20g，磷酸二氢钾2-3g，磷酸氢二钾2-3g，硫酸镁2-3g，碳酸钙1-2g，硫酸亚铁1-2g，搅拌均匀，定容至1L，制得驯化培养基；
4）将产黄纤维单胞菌按照常规培养获得种子液，再按照1:10的体积比转入步骤3）制备的驯化培养基，培养12-16小时，得到产黄纤维单胞菌驯化培养液；
所述枯草芽孢杆菌驯化液按照如下工艺制备而得：
5) 取羟甲基纤维素废水，往废水中添加葡萄糖30-40g，酵母粉15-20g，磷酸二氢钾2-3g，磷酸氢二钾2-3g，硫酸镁2-3g，碳酸钙1-2g，硫酸亚铁1-2g，搅拌均匀，定容至1L，制得菌株驯化培养基；
6)将枯草芽孢杆菌按照常规培养获得种子液，再按照1:15的体积比转入步骤5）的菌株驯化培养基，培养18-24小时，得到枯草芽孢杆菌驯化培养液；
所述黑曲霉驯化液按照如下工艺制备而得：
7) 取羟甲基纤维素废水，往废水中添加葡萄糖30-40g，酵母粉15-20g，磷酸二氢钾2-3g，磷酸氢二钾2-3g，硫酸镁2-3g，碳酸钙1-2g，硫酸亚铁1-2g，搅拌均匀，定容至1L，制得菌株驯化培养基；
8)将黑曲霉按照常规培养获得种子液，再按照1:10的体积比转入步骤7）的菌株驯化培养基，培养18-24小时，得到黑曲霉驯化培养液；
所述多变鱼腥藻为 ATCC 29413、产黄纤维单胞菌为ATCC 482、所述枯草芽孢杆菌为ATCC 6633、黑曲霉为ATCC 16404。
2.权利要求1所述的微生物制剂在修复羟基甲纤维素废水中的应用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710544293.3A CN107254412B (zh) | 2017-07-06 | 2017-07-06 | 一种用于修复羟甲基纤维素废水的微生物制剂及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710544293.3A CN107254412B (zh) | 2017-07-06 | 2017-07-06 | 一种用于修复羟甲基纤维素废水的微生物制剂及其制备方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107254412A CN107254412A (zh) | 2017-10-17 |
| CN107254412B true CN107254412B (zh) | 2020-03-13 |
Family
ID=60024886
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710544293.3A Active CN107254412B (zh) | 2017-07-06 | 2017-07-06 | 一种用于修复羟甲基纤维素废水的微生物制剂及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107254412B (zh) |
-
2017
- 2017-07-06 CN CN201710544293.3A patent/CN107254412B/zh active Active
Non-Patent Citations (4)
| Title |
|---|
| production,purification,and characterization of carboxymethyl cellulase from novel strain bacillus megaterium;zeeshan hussain shahid et al.;《aiche》;20160622;全文 * |
| 产黄纤维单孢菌CR-14菌株诱变选育及发酵条件优化;杨继业等;《农业资源与环境学报》;20150531;第33卷(第3期);全文 * |
| 微生物混菌发酵应用研究进展;徐颖宣等;《中国酿造》;20081231(第9期);全文 * |
| 高效降解生活污水COD混合菌株的筛选及固定化研究;张彬彬等;《环境科技》;20120228;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107254412A (zh) | 2017-10-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104529116B (zh) | 一种利用纳米四氧化三铁提高厌氧消化产甲烷菌活性与产甲烷效率的方法 | |
| Siddeeg et al. | Agro-industrial waste materials and wastewater as growth media for microbial bioflocculants production: a review | |
| CN102718325B (zh) | 培养高密度油脂微藻处理酵母工业废水的方法 | |
| CN104140935A (zh) | 一株具有脱氮功能的红球菌及其菌剂的生产方法和应用 | |
| CN101225405A (zh) | 一种微生物絮凝剂的生产方法及使用方法 | |
| CN108070544A (zh) | 门多萨假单胞菌及其培养基、发酵方法和应用 | |
| WO2021077453A1 (zh) | 一种施氏假单胞菌、采用该施氏假单胞菌制得的复合菌剂及该复合菌剂的应用 | |
| CN102745821B (zh) | 用于污泥减量的复合微生物菌剂及其制备方法和应用 | |
| CN107325982B (zh) | 利用微生物制剂深度处理羟甲基纤维素生产废水的工艺 | |
| CN105002127A (zh) | 复合菌剂及其在大蒜加工废水处理中的应用 | |
| CN104071902A (zh) | 一种处理生活污水的方法 | |
| CN105948243A (zh) | 一种快速培养适于制药废水处理的厌氧颗粒污泥的方法 | |
| CN102533890A (zh) | 一种赖氨酸的生产方法 | |
| CN109019874B (zh) | 一种用于造纸废水的生物促生剂及其制备方法 | |
| CN107254412B (zh) | 一种用于修复羟甲基纤维素废水的微生物制剂及其制备方法 | |
| CN109468251B (zh) | 一株硫脲降解菌株及应用该菌株处理含硫脲废水的方法 | |
| CN103952447A (zh) | 一种利用厌氧条件下发酵生产丁二酸的方法 | |
| CN112358041B (zh) | 一种同步反硝化脱氮和产甲烷除cod的颗粒污泥培养方法 | |
| CN114057291B (zh) | 一种总氮去除促生药剂及其制备和应用 | |
| CN109762849B (zh) | 一种春雷霉素残渣发酵产氢装置及其产氢方法 | |
| NZ198239A (en) | Two-stage production of ethanol by fermentation | |
| CN113548769A (zh) | 一种利用柠檬酸发酵废水制备生物复合碳源的方法和应用 | |
| CN112175879A (zh) | 一种同步异养硝化-好氧反硝化门多萨假单胞菌的培养方法及应用 | |
| CN106754548B (zh) | 一种微生物菌肥的菌株发酵方法及其应用 | |
| CN114249433B (zh) | 一种生物絮凝剂及其制法与用途 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200212 Address after: 311400 Hangzhou, Fuyang, Zhejiang province 9 Yin Hu Street, No. 9, No. 11, two, floor 206, innovation center. Applicant after: HANGZHOU FUYANG JIACHANG MACHINERY CO.,LTD. Address before: Hangzhou City, Zhejiang province 311400 Fuyang District No. 25 Fuchun Street Guihua Road 5012 room fifth Applicant before: Zhao Liqiang |
|
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200330 Address after: 264200 room 1001, block a, innovation and entrepreneurship base, No. 213-2, Torch Road, gaoqu District, Weihai City, Shandong Province Patentee after: Weihai Weinong Biotechnology Co.,Ltd. Address before: 311400 Hangzhou, Fuyang, Zhejiang province 9 Yin Hu Street, No. 9, No. 11, two, floor 206, innovation center. Patentee before: HANGZHOU FUYANG JIACHANG MACHINERY Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200812 Address after: 5-6-1, No.4, Lane 33, Yingkou West Road, Heping District, Shenyang City, Liaoning Province Patentee after: Liu Enxi Address before: 264200 room 1001, block a, innovation and entrepreneurship base, No. 213-2, Torch Road, gaoqu District, Weihai City, Shandong Province Patentee before: Weihai Weinong Biotechnology Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210728 Address after: Room 1001, block a, innovation and entrepreneurship base, 213-2 Torch Road, gaoqu District, Weihai City, Shandong Province, 264200 Patentee after: Weihai Weinong Biotechnology Co.,Ltd. Address before: 110000 5-6-1, No.4, Lane 33, Yingkou West Road, Heping District, Shenyang City, Liaoning Province Patentee before: Liu Enxi |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A microbial preparation and preparation method for repairing hydroxymethylcellulose wastewater Effective date of registration: 20230517 Granted publication date: 20200313 Pledgee: Weihai City Commercial Bank Limited by Share Ltd. high tech branch Pledgor: Weihai Weinong Biotechnology Co.,Ltd. Registration number: Y2023980040846 |