CN107247062A - One kind freezing ESEM sample cryopreservation device - Google Patents
One kind freezing ESEM sample cryopreservation device Download PDFInfo
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- CN107247062A CN107247062A CN201710419870.6A CN201710419870A CN107247062A CN 107247062 A CN107247062 A CN 107247062A CN 201710419870 A CN201710419870 A CN 201710419870A CN 107247062 A CN107247062 A CN 107247062A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
- G01N23/2202—Preparing specimens therefor
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Abstract
The present invention relates to experiment apparatus technical field, more particularly to a kind of freezing ESEM sample cryopreservation device.When progress sample freezes, the sample cryopreservation device is positioned in liquid nitrogen container, the sample cryopreservation device includes luggage carrier and multiple sample boxs, and the luggage carrier is axially inside sequentially provided with multiple independent sample rooms, and the sample box is at least partly positioned in the corresponding sample room.The sample cryopreservation device, its sample box is each respectively placed in corresponding independent sample room, it is easy to the classification, management and transfer of sample, sample can deposit the longer time in liquid nitrogen container, meet the requirement of sample Long-term Cryopreservation, and fresh liquid nitrogen need not be continuously replenished, save the consumption of amount of liquid nitrogen, reduce financial cost and the maintenance cost of personnel, and sample is in transfer process, all the time it is in liquid nitrogen, sample surfaces frosting will not be made or crackle occur, the damage in sample transfer process is alleviated.
Description
Technical field
The present invention relates to experiment apparatus technical field, more particularly to a kind of freezing ESEM sample cryopreservation device.
Background technology
Freeze scanning electron microscopy directly can carry out microcosmic imaging to metal, chemistry and High water cut biomedical material, its
Resolution ratio between transmission electron microscope and light microscope, be develop rapidly 1980s with wide variety of method,
It is one of the important research instrument in life field.It is vacuum environment to freeze ESEM chamber interior, if by the biology of High water cut
Sample, which is directly placed into sample room, can cause sample dehydration to deform, and influence ortho states topographical information, and conventional fixation, dehydration, critical
Point the method such as dries because step is lengthy and tedious, requires higher to the technical merit of operating personnel, experiment it is repeated poor.Work is former
Manage and rapidly cooled down for biomaterial is put into (- 210 DEG C) in liquid nitrogen slush, the structure and chemical composition of biological tissue cell can be made
Close to animation, the water in sample is changed into glassy state by liquid, the original appearance information of sample is remained to greatest extent.Through
Again by processing such as distillation etching, plated films after transmitting device transfer, it is finally putting into cold in the Electronic Speculum chamber that temperature is -130 DEG C
On platform, observe under vacuum.Freezing ESEM goes out the electronic signal of sample surfaces using electron-beam excitation, then through specific
Detector, such as secondary electron detector, backscatter detector detect that electron probe is produced in after sample interaction
Signal, converts optical signals into electric signal, and carries out preposition amplification and video amplifier, then electric signal is transformed into after voltage signal
It is delivered to display.
Because special condition is limited, when the large-scale plant sampling amount of such as sorghum is big, it is impossible to which whole samples are removed to laboratory
It is interior, or observation stomata by illumination effect opening and closing degree when, transportation may cause experimental data forbidden, or sample time and
Instrument use time is inconsistent etc., freezing ESEM materials can not in laboratory under the conditions of complete, being required for must by sample
Palpus outdoor sampling in advance, is then placed in liquid nitrogen to classify to freeze to wait and tests.When starting experiment, then sample taken out be transferred to it is solid
In constant volume device, the whole sample surfaces of experiment are not readily accessible to air, otherwise easily occur to produce ice crystal or crackle, shadow in sample surfaces
Ring final experimental result.
Mode is frozen using what upper experimenter unified at present, substantially there are 3 kinds：(1) sample is wrapped with aluminium-foil paper,
Numbering is write in outside, is frozen in the liquid nitrogen container for then direct plungeing into 200mm bores；(2) sample is classified and put into equipped with liquid nitrogen
50ml EP pipes, then freeze in the liquid nitrogen container of overall input 200mm bores；(3) sample classification is put into equipped with liquid nitrogen
50ml EP pipes, then freeze in the overall thermos cup for putting into 200mm bores.
There is following defect in the current mode that freezes：
First, it is impossible to quickly search sample.Sample is wrapped or put into EP pipes with aluminium-foil paper, although there is classification outside
Mark, but final is all to mix to put in liquid nitrogen container.If sample classification is a lot, it is necessary to ransackd in liquid nitrogen, it has not been convenient to quick
Search sample.
Secondly, the time for freezing sample is shorter, long-term to preserve increase maintenance cost.Because sample is all larger in opening
Deposited in liquid nitrogen container, liquid nitrogen volatilization is very fast, open liquid nitrogen container is general can not to meet conditions of cryopreservation once all left and right.If it is desired to
It is long-term to preserve, it is necessary to be continuously replenished fresh liquid nitrogen, and personnel constantly to notice remaining amount of liquid nitrogen number, add it is economical and
The maintenance cost of personnel.
Again, sample is vulnerable to damage in transfer process, shows：(1) aluminium foil paper bag storage sample product mode：Sample is through liquid
Nitrogen freezes rear embrittlement, especially blade class sample, and transfer process is easy to that sample can be broken into pieces；(2) EP pipes packaged sample mode：
Sample is integrally poured out during sampling, blade class sample is easy to meeting wall built-up when pouring out, and contacts the sample with air, produces ice crystal
Or crackle, ice crystal can excessively increase the degree of later stage distillation operation lesioned sample, and the generation later stage of crackle can not make up.
The content of the invention
(1) technical problem to be solved
ESEM sample cryopreservation device is freezed the technical problem to be solved in the present invention is to provide one kind, it extends sample
Product freeze the time, and are easy to freeze the transfer and classification of sample.
(2) technical scheme
In order to solve the above-mentioned technical problem, ESEM sample cryopreservation device is freezed the invention provides one kind, entered
When row sample freezes, it is placed in liquid nitrogen container, the cryopreservation device is included in luggage carrier and multiple sample boxs, the luggage carrier
Portion is sequentially provided with multiple independent sample rooms vertically, and the sample box at least partly (i.e. a part for single sample box) is placed
In in the corresponding sample room.When freezing sample, after cryopreservation device assembling is finished, it is put in liquid nitrogen container.
Further, the luggage carrier is axially inside sequentially provided with the loading of multiple axis perpendiculars with luggage carrier
The sample room is constituted between plate, two adjacent support boards.
Further, the support board is fastened in luggage carrier, and it is shaped as matching with the cross section of luggage carrier.
Further, elastic spacing piece is provided with the sample room, the elastic spacing piece integrally reclines the sample room
One of support board, the elastic spacing piece includes flat end and protruding end, the protruding end it is raised towards the sample room
Another support board.
It is preferred that, the protruding end of the elastic spacing piece is located at the middle part of the support board, and the flat end is fixed on load
On thing plate (being preferred to use bolt to fix).
Further, the sample box includes box body and is covered in the lid on the box body top, the lid be provided with
The perforate that the protruding end of the elastic spacing piece matches, when the sample box is placed in sample room, the protruding end and institute
State perforate to be interlocked, the sample box is fixed in sample room.It is preferred that being respectively provided with one and institute at the relative two ends of lid
The perforate that protruding end matches is stated, the perforate can also be multiple, or lid is designed as into Openworks shape, act as coordinating bullet
Property banking stop fix sample box.
Further, the side wall top of the box body is provided with least one groove, and dress is frozen taking out this out of liquid nitrogen container
When putting, a little liquid nitrogen is poured out by the groove, to reduce during hand taking sample box by the possibility of liquid nitrogen frostbite.
Further, the tray interior is provided with filter screen.
It is preferred that, the filter screen is provided with arc shaped handle, in order to be taken with tweezers, and the arc height of design is as far as possible low, with
It can be limited with tweezers gripping；The side wall of the box body is provided with least three screw holes, and the screw hole is combined with the spiral shell of standard
Adjusting screw, by screw by screen support in box body.
Further, spaced between the adjacent sample room, the activity reserved at intervals of sample box of taking is empty
Between.
Further, the shape of the sample box is placed in sample room part and sample room matches.
Further, the cross section of the sample box is circular or square, and the cross section of the sample room is in and the sample
The circular arc (being preferably semicircle) or square that the shape of cross section of product box matches.
Further, the luggage carrier is provided with handle, and the top of the handle is provided with hook, and the crotch end of the hook is used
In being fixed on liquid nitrogen tank mouth；And/or, the quantity of the sample room is 4.
(3) beneficial effect
The above-mentioned technical proposal of the present invention has the advantages that：
1st, freezing ESEM of the invention includes luggage carrier and multiple sample boxs, the luggage carrier with sample cryopreservation device
Multiple independent sample rooms are axially inside sequentially provided with, the sample box is partly or entirely positioned over the corresponding sample room
It is interior.When freezing sample, plunge the sample into the sample box for filling liquid nitrogen, then the sample box be placed in sample room, you can
Cryopreservation device assembling is finished, is put in liquid nitrogen container.Because sample box is each respectively placed in corresponding independent sample
Interior, therefore sample is individually separated when freezing, consequently facilitating classification and shifting.Take when freezing sample, wear gloves operation i.e.
Can, the sample for the needs that can quickly and easily take, it is clear that sample freezes classification.
2nd, the cryopreservation device compact conformation of the invention, need to only be frozen this by the thinner opening of liquid nitrogen container when freezing
Device is put into inside liquid nitrogen container, and liquid nitrogen container is without larger open, if the placement of cryopreservation device is disclosure satisfy that, thus significantly
The volatilization of liquid nitrogen is alleviated, sample can be deposited in liquid nitrogen container up to two months, meet the requirement of sample Long-term Cryopreservation, and
And fresh liquid nitrogen need not be continuously replenished, the consumption of amount of liquid nitrogen is saved, financial cost and the maintenance cost of personnel is reduced.
3rd, sample of the invention is when freezing state without other containers, and sample is preserved in semi-closed state, is carried
Rack will not be because sample mix occurs for liquid-nitrogen boiling when entering in liquid nitrogen container.Also, sample is in transfer process, it is in all the time
In liquid nitrogen, sample surfaces frosting will not be made or crackle occur, the damage in sample transfer process is alleviated.
4th, luggage carrier of the invention can be according to needing to set multiple sample rooms, and then accommodates multiple sample boxs, and freezes
Cryopreservation device compact conformation, same liquid nitrogen container can accommodate multiple luggage carriers, disclosure satisfy that freezing for a large amount of samples not of the same race.
Brief description of the drawings
Fig. 1 is the structural representation of sample cryopreservation device described in the embodiment of the present invention；
Fig. 2 is the structural representation of luggage carrier described in the embodiment of the present invention；
Fig. 3 is the structural representation of box body described in the embodiment of the present invention；
Fig. 4 is the structural representation of filter screen described in the embodiment of the present invention；
Fig. 5 is the structural representation of lid described in the embodiment of the present invention；
Wherein, 1, luggage carrier；2nd, sample box；3rd, filter screen；4th, lid；5th, support board；9th, elastic spacing piece；10th, arc is carried
Hand；11st, perforate；12nd, groove；13rd, screw hole；17th, link up with；19th, sample room；24th, handle；25th, box body；26th, flat end；27、
Protruding end.
Embodiment
Embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples.Following examples are used for
Illustrate the present invention, but can not be used for limiting the scope of the present invention.
In the description of the invention, unless otherwise indicated, " multiple " are meant that two or more；Term " on ",
" under ", "left", "right", " interior ", " outer ", " top ", the orientation of the instruction such as " bottom " or position relationship be based on shown in the drawings
Orientation or position relationship, are for only for ease of the description present invention and simplify description, rather than indicate or imply signified device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.This
Outside, term " first ", " second ", " the 3rd " etc. are only used for describing purpose, and it is not intended that indicating or implying relative importance.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；Can
To be mechanical connection or electrical connection；Can be joined directly together, can also be indirectly connected to by intermediary.For this
For the those of ordinary skill in field, concrete condition can be regarded and understand the concrete meaning of above-mentioned term in the present invention.
As shown in Fig. 1~5, a kind of freezing ESEM sample cryopreservation device is present embodiments provided, sample is being carried out
When freezing, it is placed in liquid nitrogen container (not shown), the cryopreservation device includes the luggage carrier 1 and four samples of semi-circular cylindrical
Product box 2, the luggage carrier 1 is axially inside sequentially provided with four independent sample rooms 19, a part for each sample box 2
It is positioned in the corresponding sample room 19, another part is retained in outside sample room 19.When freezing sample, by the cryopreservation device
After assembling is finished, it is put in liquid nitrogen container.
Specifically, there is interval between the adjacent sample room 19, the activity reserved at intervals of sample box 2 of taking is empty
Between.The luggage carrier 1 is axially inside sequentially provided with the support board 5 of eight and the axis perpendicular of luggage carrier 1, two adjacent institutes
State and constitute the sample room 19 between support board 5.The support board 5, which is fastened in luggage carrier 1, (specifically to be blocked in the present embodiment and sets
Mode is welding), and it is shaped as the semicircle that matches with the cross section of luggage carrier 1, so that the luggage carrier 1 and support board
5 collectively form the structure of semi-cylindrical.
Elastic spacing piece 9 is provided with the sample room 19, the elastic spacing piece 9 is overall to recline the sample room 19 wherein
One support board 5, the elastic spacing piece 9 includes flat end 26 and protruding end 27, the protruding end 27 it is raised towards the sample
Another support board 5 of product room 19.In the present embodiment, the flat end 26 of the elastic spacing piece 9 is respectively positioned on semicircle sample room
19 inner side, and adopt and be bolted to support board 5, the protruding end 27 of the elastic spacing piece 9 is located at the middle part of support board 5.
The sample box 2 includes box body 25 and is covered in the lid 4 on the top of box body 25, and the inside of box body 25 is provided with
Filter screen 3.Specifically, the filter screen 3 is provided with arc shaped handle 10, in order to be taken with tweezers, the arc height of design is as far as possible low, with
It can be limited with tweezers gripping, to ensure effectively freezing highly for sample box 2；Interval is uniformly provided with four on the side wall of the box body 25
Individual screw hole 13, the screw hole 13 is combined with the screw (not shown) of standard, is supported on filter screen 3 by screw
In box body 25, filter screen 3 is placed on the screw of the correspondence position of screw hole 13 of sample box 2 during installation.In the present embodiment, institute
State height of the screw hole 13 apart from the bottom surface of sample box 2 and be not less than the rim of a cup height 5mm that freezing ESEM shifts cup, otherwise exist
Easily make sample exposure air when scooping out sample, cause the phenomenon of sample frosting or crackle.The material of the filter screen 3 is stainless
Steel, intermediate body portions are divided into gauze, and mesh mesh number (i.e. the aperture of filter screen 3) requirement is not more than the size for freezing sample, the gauze
Designed for the flanging of filter screen 3 at edge.
The lid 4 is provided with the perforate 11 matched with the protruding end 27 of the elastic spacing piece 9, when the sample box 2
When being placed in sample room 19, the protruding end 27 is interlocked with the perforate 11, and the sample box 2 is fixed on into sample room 19
It is interior.The present embodiment is preferably respectively provided with a perforate 11 matched with the protruding end 27, this two at the relative two ends of lid 4
Perforate 11 is symmetrical arranged, and the perforate 11 can also be multiple, or lid 4 is designed as into Openworks shape, act as coordinating elasticity
Banking stop 9 fixes sample box 2.When cryopreservation device is installed, lid 4 is buckled on box body 25, then by elastic spacing piece 9
The fastening of pressure and elastic spacing piece 9 and lid 4, sample box 2 is integrally fastened in the sample room 19 of luggage carrier 1.
The shape of part and sample room 19 that the sample box 2 is placed in sample room 19 matches, described in the present embodiment
Sample box 2 is circle, and its diameter is equal with the internal diameter of semicircle sample room 19, and the diameter of semi-circular cylindrical luggage carrier 1 and the sample
The cross sectional dimensions (i.e. diameter) of product box 2 is respectively less than the bore of the liquid nitrogen container.
The side wall top interval of the sample box 2 is uniformly provided with four grooves 12, and dress is frozen taking out this out of liquid nitrogen container
When putting, a little liquid nitrogen is poured out by the groove 12, to reduce by the possibility of liquid nitrogen frostbite during hand taking sample box 2, groove 12
Depth be difficult it is excessive, with meet take when be difficult to hinder hand.The sidewall weld of the top sample room 19 of the luggage carrier 1 has
Handle 24, the top of the handle 24 is provided with hook 17, and the crotch end of the hook 17 is used to luggage carrier 1 being fixed on liquid nitrogen container
Mouthful.
In the present embodiment, the material of the luggage carrier 1 and support board 5 is stainless steel；The material of the sample box 2 is
Aluminium, the diameter of the circular sample box 2 is more than diameter 25mm of the freezing ESEM with transfer cup, and have enough spaces so as to
The sample frozen in advance is scooped out with transfer cup, to avoid making sample not ingress of air in transfer process, the sample box 2 of design
Internal diameter is less than the internal diameter of sample room 19；The diameter of the semicircle support board 5 is preferred more than 40mm, i.e., can match more than 40mm
The liquid nitrogen container of bore freezes；The present embodiment sets 4 sample rooms 19 for being used to accommodate sample box 2, due to the continuous volatilization of liquid nitrogen,
Storage space is more to shorten the resting period, otherwise can reduce storing samples quantity.
Liquid nitrogen cryopreservation is operated：When freezing sample, the full-size that sample is frozen in principle is less than the internal diameter of sample box 2, will
Sample is direct plungeed into the box body 25 of sample box 2 for filling liquid nitrogen, is capped filter screen 3, lid 4 is then capped again.By groove 12, slightly
Low dip pours out a little liquid nitrogen, conveniently takes, and sample box 2 is positioned in sample room 19 by band gloves operation, by elastic spacing
Piece 9 and the cooperation of the perforate 11 of lid 4, the installation for completing a sample box 2 are fixed, and the operation of other three sample boxs 2 is similar,
Repeat no more.After the fixation for completing four sample boxs 2, luggage carrier 1 is put into liquid nitrogen container and freezes wait experiment.
Operation is taken out in experiment：When taking out sample, luggage carrier 1 is relied on into groove 12, is slightly tilted and pours out a little liquid nitrogen, it is convenient
Take.Wear gloves to take out the direct level of sample box 2 of one of position, on the mat applying for being positioned over thermal insulation, taken away with tweezers
Lid 4 and filter screen 3.The sample of needs is scooped out with transfer cup, the transfer that transfer cup is transferred to freezing ESEM is gripped with tweezers
In device, complete to freeze the taking-up action of sample.
In summary, the sample cryopreservation device described in the present embodiment, its sample box is each respectively placed in corresponding independence
Sample room in, therefore sample is individually separated when freezing, consequently facilitating classification and shifting.Take when freezing sample, wear gloves
Operation, the sample for the needs that can quickly and easily take, it is clear that sample freezes classification.The cryopreservation device compact conformation, freezes
The cryopreservation device need to be only put into when depositing inside liquid nitrogen container by the thinner opening of liquid nitrogen container, significantly reduce waving for liquid nitrogen
Hair, sample can be deposited in liquid nitrogen container up to two months, meet the requirement of sample Long-term Cryopreservation, and need not be continuously replenished
Fresh liquid nitrogen, saves the consumption of amount of liquid nitrogen, reduces financial cost and the maintenance cost of personnel.Sample when freezing state without
Other containers are needed, sample is preserved in semi-closed state, and luggage carrier will not be sent out when entering in liquid nitrogen container due to liquid-nitrogen boiling
Raw sample mix.And sample is in transfer process, all the time in liquid nitrogen, sample surfaces frosting will not be made or crackle occur.
Embodiments of the invention are provided for the sake of example and description, and are not exhaustively or by this to send out
It is bright to be limited to disclosed form.Many modifications and variations are obvious for the ordinary skill in the art.Choosing
Select and describe embodiment and be more preferably to illustrate the principle and practical application of the present invention, and make one of ordinary skill in the art
It will be appreciated that the present invention is so as to design the various embodiments with various modifications suitable for special-purpose.
Claims (10)
1. one kind freezing ESEM sample cryopreservation device, it is characterised in that including luggage carrier and multiple sample boxs, the load
Rack is axially inside sequentially provided with multiple independent sample rooms, and the sample box is at least partly positioned over the corresponding sample
It is indoor.
2. freezing ESEM sample cryopreservation device according to claim 1, it is characterised in that inside the luggage carrier
It is sequentially provided with vertically between the support board of multiple axis perpendiculars with luggage carrier, two adjacent support boards described in constituting
Sample room.
3. freezing ESEM sample cryopreservation device according to claim 2, it is characterised in that the loading board is set
In luggage carrier, and the cross section of its shape and luggage carrier matches.
4. freezing ESEM sample cryopreservation device according to claim 3, it is characterised in that set in the sample room
Flexible banking stop, the elastic spacing piece integrally reclines one of support board of the sample room, the elastic spacing piece bag
Include flat end and protruding end, raised another support board towards the sample room of the protruding end.
5. freezing ESEM sample cryopreservation device according to claim 4, it is characterised in that the sample box includes
Box body and the lid for being covered in the box body top, the lid are provided with and opening that the elastic spacing piece protruding end matches
Hole, when the sample box is placed in sample room, the protruding end is interlocked with the perforate, and the sample box is fixed on
In sample room.
6. freezing ESEM sample cryopreservation device according to claim 4, it is characterised in that the side wall of the box body
Top is provided with least one groove.
7. freezing ESEM sample cryopreservation device according to claim 4, it is characterised in that the tray interior is set
There is filter screen.
8. the freezing ESEM sample cryopreservation device according to any one of claim 1~7, it is characterised in that adjacent
It is spaced between the sample room.
9. freezing ESEM sample cryopreservation device according to claim 8, it is characterised in that the horizontal stroke of the sample box
Section to be circular or square, the cross section of the sample room be in the circular arc that matches with the shape of cross section of the sample box or
It is square.
10. freezing ESEM sample cryopreservation device according to claim 1, it is characterised in that the loading is set up
There is handle, the top of the handle is provided with hook；And/or, the quantity of the sample room is 4.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710419870.6A CN107247062B (en) | 2017-06-06 | 2017-06-06 | A kind of freezing scanning electron microscope sample cryopreservation device |
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| CN201710419870.6A CN107247062B (en) | 2017-06-06 | 2017-06-06 | A kind of freezing scanning electron microscope sample cryopreservation device |
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| CN107247062B CN107247062B (en) | 2019-04-19 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110542259A (en) * | 2019-08-09 | 2019-12-06 | 河南中镜科仪科技有限公司 | Quick freezing device |
| CN111175329A (en) * | 2020-01-16 | 2020-05-19 | 中国石油大学(北京) | In-situ observation method for microstructure of liquid nitrogen frozen rock |
| CN111855720A (en) * | 2019-04-26 | 2020-10-30 | 中国科学院广州能源研究所 | Novel frozen scanning electron microscope sample stage and sampling method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006097720A1 (en) * | 2005-03-16 | 2006-09-21 | Cambridge Magnetic Refrigeration, Ltd. | Sample support with tilting and rotating means for cryogenic applications |
| CN201044541Y (en) * | 2007-04-26 | 2008-04-02 | 环达电脑(上海)有限公司 | Latching structure of case of electronic device |
| CN202368216U (en) * | 2011-11-04 | 2012-08-08 | 吕丽 | Improved multifunctional pen container |
| CN203865247U (en) * | 2014-05-25 | 2014-10-08 | 新疆维吾尔自治区人民医院 | Sample storing and taking device for liquid nitrogen container |
| CN104764651A (en) * | 2015-03-30 | 2015-07-08 | 广东工业大学 | Liquefied nitrogen tank |
| CN105857932A (en) * | 2016-04-28 | 2016-08-17 | 上海原能健康管理有限公司 | Liquid nitrogen container and cryogenic vial pick-and-place device |
| CN205593836U (en) * | 2016-01-27 | 2016-09-21 | 北京市农林科学院 | A freeze -drying board and device for rapid freeze thawing sample |
-
2017
- 2017-06-06 CN CN201710419870.6A patent/CN107247062B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006097720A1 (en) * | 2005-03-16 | 2006-09-21 | Cambridge Magnetic Refrigeration, Ltd. | Sample support with tilting and rotating means for cryogenic applications |
| CN201044541Y (en) * | 2007-04-26 | 2008-04-02 | 环达电脑(上海)有限公司 | Latching structure of case of electronic device |
| CN202368216U (en) * | 2011-11-04 | 2012-08-08 | 吕丽 | Improved multifunctional pen container |
| CN203865247U (en) * | 2014-05-25 | 2014-10-08 | 新疆维吾尔自治区人民医院 | Sample storing and taking device for liquid nitrogen container |
| CN104764651A (en) * | 2015-03-30 | 2015-07-08 | 广东工业大学 | Liquefied nitrogen tank |
| CN205593836U (en) * | 2016-01-27 | 2016-09-21 | 北京市农林科学院 | A freeze -drying board and device for rapid freeze thawing sample |
| CN105857932A (en) * | 2016-04-28 | 2016-08-17 | 上海原能健康管理有限公司 | Liquid nitrogen container and cryogenic vial pick-and-place device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855720A (en) * | 2019-04-26 | 2020-10-30 | 中国科学院广州能源研究所 | Novel frozen scanning electron microscope sample stage and sampling method |
| CN110542259A (en) * | 2019-08-09 | 2019-12-06 | 河南中镜科仪科技有限公司 | Quick freezing device |
| CN111175329A (en) * | 2020-01-16 | 2020-05-19 | 中国石油大学(北京) | In-situ observation method for microstructure of liquid nitrogen frozen rock |
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| CN107247062B (en) | 2019-04-19 |
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