CN107245449A - The preparation method and its application method of a kind of fermentation medium soybean nitrogen source powder - Google Patents
The preparation method and its application method of a kind of fermentation medium soybean nitrogen source powder Download PDFInfo
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- CN107245449A CN107245449A CN201710654444.0A CN201710654444A CN107245449A CN 107245449 A CN107245449 A CN 107245449A CN 201710654444 A CN201710654444 A CN 201710654444A CN 107245449 A CN107245449 A CN 107245449A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
It is an object of the invention to provide preparation method and its application method of a kind of fermentation medium with soybean nitrogen source powder, preparation method comprises the following steps:1)Soybean Meal coarse powder;2)100 parts of Soybean Meal parts by weight are taken to be placed in mixing and blending machine;3)The soybean oil of 3 11 parts of parts by weight is taken, is delivered in mixing and blending machine;4)Stirring;5)Heating and stirring device is heat aging;6)Squeezing shaping;7)It is cooled to environment temperature;8)Pulverizing mill is crushed;9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements;Soybean nitrogen source powder is used in fermentation medium.Soybean nitrogen source powder produced by the present invention substitutes the soybean cake that conventional press method is obtained, and opens and the new manufacture field of soybean nitrogen source is obtained in a zymotechnique, expand the source of organic nitrogen source powder, change the limitation of raw material sources, and mode of production energy-conserving and environment-protective.The soybean nitrogen source powder of the application is used in fermentation medium, and reproducible, ferment effect is notable.
Description
Technical field
The present invention relates to preparation method and its application method of a kind of fermentation medium with soybean nitrogen source powder, belong to soybean nitrogen
Source powder preparing technical field.
Background technology
Containing organic nitrogen source, amino acid, lysine and thick protein etc. in soybean cake, the day in fermentation technology process is used as
Right nutrient media components is widely used, and traditional soybean cake is soybean using milling process or the byproduct rammed after the oil expression of squeezing method, cost
Higher, residual fat is about 5-7% in limited source, and the soybean cake of conventional press method production, and effective energy value and gross protein value are equal
Relatively low, amino acid content is even below same condition, Soybean Meal at the same level;Therefore the soybean cake of conventional press method production is as in hair
Ferment nutrient media components in use, need add auxiliary material it is more, wasting manpower and material resources' financial resources.
The content of the invention
In order to overcome disadvantage mentioned above, it is an object of the invention to provide a kind of preparation of fermentation medium with soybean nitrogen source powder
Method and its application method, concrete scheme is:
A kind of fermentation medium preparation method of soybean nitrogen source powder, comprises the following steps:1)Soybean Meal is taken to be positioned over pulverizer
Middle carry out coarse powder;2)100 parts of Soybean Meal parts by weight are taken to be placed in mixing and blending machine;3)Take the soybean of 3-11 parts of parts by weight
Oil, is delivered in mixing and blending machine;4)Stirring, is sufficiently mixed Soybean Meal and soybean oil;5)Heated in heating and stirring device ripe
Change;6)Squeezing shaping;7)Cooling;8)Micro mist;9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements.
The step 5)In, curing temperature is 40-200 DEG C.
Described step 5)In, it is preferred that curing temperature is 70 DEG C.
Described step 8)In, it is preferred that the granularity of fine powder is 50 mesh, 60 mesh, 70 mesh or 80 mesh.
Disclosed herein as well is a kind of application method of above-mentioned fermentation medium soybean nitrogen source powder, soybean nitrogen source powder adds
Add to and be used as nitrogen source in fermentation medium.Ferment effect is reproducible compared with the fermentation culture medium using traditional soybean cake,
Ferment effect is notable.
Beneficial effect:The invention provides a kind of preparation method of fermentation medium with soybean nitrogen source powder, using dregs of beans and
Soya-bean oil is that raw material produces soybean nitrogen source powder, substitutes traditional soybean cake, opens acquisition soybean nitrogen source in a zymotechnique new
Manufacture field, expand the source of organic nitrogen source powder, change the limitation of raw material sources.In addition, the soybean nitrogen of the application
Source powder is used in fermentation medium, reproducible, and ferment effect is notable.
Brief description of the drawings
Fig. 1 is the schematic flow sheet of preparation method of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be further described.
Embodiment 1
As shown in figure 1, a kind of fermentation medium preparation method of soybean nitrogen source powder, specifically includes following steps:
1)Take Soybean Meal to be positioned in pulverizer and carry out coarse powder;
2)100 parts of Soybean Meal parts by weight after electronic scale weighing coarse powder are placed in mixing and blending machine;
3)Weighed tank is weighed the soybean oils of 4 parts of parts by weight, is delivered in mixing and blending machine;
4)Stirring, is sufficiently mixed Soybean Meal and soybean oil;
5)After being sufficiently mixed, heat aging in heating and stirring device, 50 DEG C of curing temperature is transported to;
6)Squeezing shaping;
7)It is cooled to environment temperature;
8)Micro mist is to 80 mesh;
9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements.
Embodiment 2
As shown in figure 1, a kind of fermentation medium preparation method of soybean nitrogen source powder, specifically includes following steps:
1)Take Soybean Meal to be positioned in pulverizer and carry out coarse powder;
2)100 parts of Soybean Meal parts by weight after electronic scale weighing coarse powder are placed in mixing and blending machine;
3)Weighed tank is weighed the soybean oils of 6 parts of parts by weight, is delivered in mixing and blending machine;
4)Stirring, is sufficiently mixed Soybean Meal and soybean oil;
5)It is heat aging, 70 DEG C of curing temperature;
6)Squeezing shaping;
7)It is cooled to environment temperature;
8)Micro mist is to 60 mesh;
9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements.
Embodiment 3
As shown in figure 1, a kind of fermentation medium preparation method of soybean nitrogen source powder, specifically includes following steps:
1)Take Soybean Meal to be positioned in pulverizer and carry out coarse powder;
2)100 parts of Soybean Meal parts by weight after electronic scale weighing coarse powder are placed in mixing and blending machine;
3)Weighed tank is weighed the soybean oils of 8 parts of parts by weight, is delivered in mixing and blending machine;
4)Stirring, is sufficiently mixed Soybean Meal and soybean oil;
5)It is heat aging, 60 DEG C of curing temperature;
6)Squeezing shaping;
7)It is cooled to environment temperature;
8)Micro mist is to 50 mesh;
9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements.
Embodiment 4
As shown in figure 1, a kind of fermentation medium preparation method of soybean nitrogen source powder, specifically includes following steps:
1)Take Soybean Meal to be positioned in pulverizer and carry out coarse powder;
2)100 parts of Soybean Meal parts by weight after electronic scale weighing coarse powder are placed in mixing and blending machine;
3)Weighed tank is weighed the soybean oils of 11 parts of parts by weight, is delivered in mixing and blending machine;
4)Stirring, is sufficiently mixed Soybean Meal and soybean oil;
5)It is heat aging, 60 DEG C of curing temperature;
6)Squeezing shaping;
7)It is cooled to environment temperature;
8)Micro mist is to 70 mesh;
9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements.
Embodiment 5
As shown in figure 1, a kind of fermentation medium preparation method of soybean nitrogen source powder, specifically includes following steps:
1)Take Soybean Meal to be positioned in pulverizer and carry out coarse powder;
2)100 parts of Soybean Meal parts by weight after electronic scale weighing coarse powder are placed in mixing and blending machine;
3)Weighed tank is weighed the soybean oils of 11 parts of parts by weight, is delivered in mixing and blending machine;
4)Stirring, is sufficiently mixed Soybean Meal and soybean oil;
5)It is heat aging, 40-200 DEG C of curing temperature;
6)Squeezing shaping;
7)It is cooled to environment temperature;
8)Micro mist is to 70 mesh;
9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements.
Obtained soybean nitrogen source powder in above-mentioned several embodiments, it is added in fermentation medium, can be used as one kind
Quick-acting nitrogen sources, contribute to thalline to absorb and Rapid Fermentation.
Claims (5)
1. a kind of fermentation medium preparation method of soybean nitrogen source powder, it is characterised in that comprise the following steps:
1)Take Soybean Meal to be positioned in pulverizer and carry out coarse crushing;
2)100 parts of Soybean Meal parts by weight are taken to be placed in mixing and blending machine;
3)The soybean oil of 3-11 parts of parts by weight of weight is taken, is delivered in mixing and blending machine;
4)Stirring, is sufficiently mixed Soybean Meal and soybean oil;
5)It is heat aging in heating and stirring device;
6)Squeezing shaping;
7)It is cooled to environment temperature;
8)Crushing of Ultrafine;
9)Sieving, that is, obtain meeting the soybean nitrogen source powder of granularity requirements.
2. a kind of preparation method of fermentation medium soybean nitrogen source powder as claimed in claim 1, it is characterised in that step 5)
In, curing temperature is 40-200 DEG C.
3. a kind of preparation method of fermentation medium soybean nitrogen source powder as claimed in claim 2, it is characterised in that step 5)
In, curing temperature is 70 DEG C.
4. a kind of preparation method of fermentation medium soybean nitrogen source powder as claimed in claim 1, it is characterised in that the step
Rapid 8)In, the granularity of soybean nitrogen source powder is 50 mesh, 60 mesh, 70 mesh or 80 mesh after micro mist.
5. a kind of application method of fermentation medium soybean nitrogen source powder as described in claim any one of 1-4, its feature exists
In soybean nitrogen source powder, which is added in fermentation medium, is used as nitrogen source.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117969862A (en) * | 2024-04-02 | 2024-05-03 | 中国农业科学院农产品加工研究所 | Screening method and application of soybean protein as microbial culture nitrogen source |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1353948A (en) * | 2000-11-22 | 2002-06-19 | 石晓岭 | Soybean full-cream protein feed and its production process |
CN101218956A (en) * | 2007-01-08 | 2008-07-16 | 石晓岭 | Process for manufacturing high-energy maize protein meal |
WO2011095875A2 (en) * | 2010-02-05 | 2011-08-11 | Council Of Scientific & Industrial Research | A novel fungal strain beauveria sp. mtcc 5184 and a process for the preparation of enzymes therefrom |
CN103391997A (en) * | 2010-02-26 | 2013-11-13 | 科学和工业研究委员会 | Enzymes from conidiobolus brefeldianus and process for preparation thereof |
-
2017
- 2017-08-03 CN CN201710654444.0A patent/CN107245449A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1353948A (en) * | 2000-11-22 | 2002-06-19 | 石晓岭 | Soybean full-cream protein feed and its production process |
CN101218956A (en) * | 2007-01-08 | 2008-07-16 | 石晓岭 | Process for manufacturing high-energy maize protein meal |
WO2011095875A2 (en) * | 2010-02-05 | 2011-08-11 | Council Of Scientific & Industrial Research | A novel fungal strain beauveria sp. mtcc 5184 and a process for the preparation of enzymes therefrom |
CN103391997A (en) * | 2010-02-26 | 2013-11-13 | 科学和工业研究委员会 | Enzymes from conidiobolus brefeldianus and process for preparation thereof |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN117969862A (en) * | 2024-04-02 | 2024-05-03 | 中国农业科学院农产品加工研究所 | Screening method and application of soybean protein as microbial culture nitrogen source |
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Application publication date: 20171013 |