CN107242564A - Gorgon fruit shell extract and its preparation method and application - Google Patents
Gorgon fruit shell extract and its preparation method and application Download PDFInfo
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- CN107242564A CN107242564A CN201710378952.0A CN201710378952A CN107242564A CN 107242564 A CN107242564 A CN 107242564A CN 201710378952 A CN201710378952 A CN 201710378952A CN 107242564 A CN107242564 A CN 107242564A
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- gorgon fruit
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to technical field of natural product extraction, and in particular to a kind of Gorgon fruit shell extract and its preparation method and application.The preparation method of the Gorgon fruit shell extract includes step：1) Gorgon fruit shell is digested, obtains digesting mixture；2) by step 1) obtained enzymolysis mixture directly carries out homogenate extraction, obtains extract solution；3) by step 2) obtained extract solution concentrates and is freeze-dried through suction filtration, heating successively, obtains Gorgon fruit shell extract.The Gorgon fruit shell extract prepared by the preparation method has the effect of obvious oxidation and removing free radicals.Meanwhile, the Gorgon fruit shell extract kills rope silk bacterium, lactic acid bacteria etc. with significant bacteriostasis to staphylococcus aureus, Escherichia coli, the western watt Salmonella of corruption, heat.The Gorgon fruit shell extract has good application prospect as natural anti-oxidant, antibacterial substance safely, effectively, in the natural additive for foodstuff of low toxicity.
Description
Technical field
The invention belongs to technical field of natural product extraction, and in particular to a kind of Gorgon fruit shell extract and preparation method thereof and
Using.
Background technology
Gorgon fruit is also known as also known as yellow, the chicken head fruit of current etc., the annual large-scale aquatic herbaceous plant of Nymphaeceae, be distributed in East Asia,
South Asia and the Southeast Asia zone of constant temperature and subtropical zone, originate in the ground such as Hubei, Hunan, Jiangxi, Anhui China more.Gorgon fruit is traditional food
Product are also good Chinese medicine,《Compendium of Materia Medica》Record：Gorgon fruit quenches the thirst kidney-nourishing.Urinary incontinence is controlled, is passed out semen, under band.It is put into me
" dietotherapeutic " plant list that the Ministry of Public Health of state announces in the first batch.
Gorgon fruit shell is the tough outer outside Gorgon fruit kernel, and the research on Gorgon fruit shell is less.Pass through Literature Consult, publication number
For CN105147781A Chinese patent disclose " a kind of scorching anti-allergy preparation of suppression using Gorgon fruit shell extract as main component and its
Using ", the patent be related to it is a kind of by the Gorgon fruit shell extract extracted by pure water or different concentration ethanol add honeycomb Aqueous extracts
Vitamin C, after three kinds of compositions are mixed according to a certain ratio, obtains a kind of scorching antiallergic system of suppression using Gorgon euryale kind shell extract as main component
Agent, it has the effect for alleviating scytitis especially allergic dermatitis.Publication No. CN105713419A Chinese patent is public
Open " preparation method and its colouring method of a kind of high-purity natural dye of gordon euryale seed shell ", the patent is related to a kind of by overcritical extraction
Gorgon fruit shell extract after taking obtains high-purity natural dye of gordon euryale seed shell by extracting.Publication No. CN104489147A China
Patent is disclosed " a kind of Gorgon fruit tea and preparation method thereof ", the patent be related to it is a kind of by aqueous Gorgon fruit shell pass through after gas explosion crush
Gorgon fruit shell frying afterwards, obtains a kind of hypoglycemic Gorgon fruit tea of reducing blood lipid.Gorgon fruit can be completed by gordon euryale seed sheller in production
Shell, but substantial amounts of Gorgon fruit shell is born as Gorgon fruit processing enterprise, is simply discarded mostly.At present on Gorgon fruit shell active ingredient
Extract and prepare and the domestic and international almost blank of bioactivity research.Gorgon fruit shell resource is developed and utilized, its functional component is extracted
Study its bioactivity and application is urgent problem to be solved.
The content of the invention
To solve the deficiencies in the prior art, the invention provides a kind of Gorgon fruit shell extract and its preparation method and application.
The present invention extracts Gorgon fruit shell extract by ad hoc approach, and the Gorgon fruit shell extract prepared has good antioxidation activity
And fungistatic effect, it can be applied to food and field of health care products.
Technical scheme provided by the present invention is as follows：
A kind of preparation method of Gorgon fruit shell extract, comprises the following steps：
1) Gorgon fruit shell is digested, obtains digesting mixture；
2) by step 1) obtained enzymolysis mixture directly carries out homogenate extraction, obtains extract solution；
3) by step 2) obtained extract solution concentrates and is freeze-dried through suction filtration, heating successively, obtains Gorgon fruit shell extract.
Specifically, step 2) in, the extract solution of homogenate extraction is the ethanol water of water or volume fraction below 80%.
Specifically, step 1) in：Gorgon fruit shell is digested with the aqueous solution of cellulase, cellulase and Gorgon fruit shell
Mass ratio be 0.8~1.2%, enzymolysis time be 90~150min, hydrolysis temperature be 40~50 DEG C, enzymolysis liquid pH value be 4.0~
5.0。
Specifically, step 2) in：The extraction voltage of homogenate extraction is 80~120V, extraction time 2 of homogenate extraction~
3min, homogenate extraction is twice.
For the preparation method of Gorgon fruit shell extract provided by the present invention：Its for Gorgon fruit hülle cell wall it is main into
Point be cellulose, mechanical crushing method is difficult to be crushed in all cells, thus using enzymolysis processing, make softening of the cell wall, it is swollen
Swollen and collapse, changes its permeability, improves the dissolution of cellular content；And then retain all products after enzymolysis, then with it is flash
Combination is extracted, extraction rapidly and efficiently is carried out at normal temperatures, is combined with enzyme process with physical method, Gorgon fruit shell is substantially increased and carries
Take the recovery rate and species of thing active ingredient.And prepared by the preparation method of Gorgon fruit shell extract provided by the present invention
Gorgon fruit shell extract there is the effects of obvious oxidation and removing free radicals.Meanwhile, the Gorgon fruit shell extract is to golden yellow Portugal
The western watt Salmonella of grape coccus, Escherichia coli, corruption, heat, which kill rope silk bacterium, lactic acid bacteria etc., has significant bacteriostasis.The Gorgon fruit shell is carried
Thing is taken as natural anti-oxidant, antibacterial substance, it is good in having safely, effectively, in the natural additive for foodstuff of low toxicity
Application prospect.
Present invention also offers the Gorgon fruit shell extract prepared according to preparation method provided by the present invention.
Gorgon fruit shell extract provided by the present invention has the effect of obvious oxidation and removing free radicals.Meanwhile, the Gorgon euryale
Real shell extract kills rope silk bacterium, lactic acid bacteria etc. with notable to staphylococcus aureus, Escherichia coli, the western watt Salmonella of corruption, heat
Bacteriostasis.The Gorgon fruit shell extract as natural anti-oxidant, antibacterial substance, safely, effectively, the wholefood of low toxicity
There is good application prospect in additive.
Present invention also offers the application of Gorgon fruit shell extract provided by the present invention, it is used as antioxidant.
Specifically, for removing DPPH free radicals, removing ABTS free radicals.
Gorgon fruit shell extract provided by the present invention has the effect of obvious oxidation and removing free radicals, and the Gorgon fruit shell is carried
Thing is taken as natural anti-oxidant, antibacterial substance, it is good in having safely, effectively, in the natural additive for foodstuff of low toxicity
Application prospect.
Present invention also offers another application of Gorgon fruit shell extract provided by the present invention, it is used as bacteriostatic agent.
Specifically, killing rope silk bacterium and/or lactic acid bacteria for suppressing the western watt Salmonella of staphylococcus aureus, corruption, heat.
Gorgon fruit shell extract provided by the present invention is killed to staphylococcus aureus, Escherichia coli, the western watt Salmonella of corruption, heat
Rope silk bacterium, lactic acid bacteria etc. have significant bacteriostasis.The Gorgon fruit shell extract as natural anti-oxidant, antibacterial substance,
Safely, effectively, there is good application prospect in the natural additive for foodstuff of low toxicity.
Brief description of the drawings
Fig. 1 is antioxidant effect of the Gorgon fruit shell alcohol extract provided by the present invention to rapeseed oil.
Fig. 2 is Gorgon fruit shell water extract provided by the present invention to rapeseed oil antioxidant effect.
The change of duck sense organ score value in Fig. 3 cold storage procedures.
The change of duck total plate count in Fig. 4 cold storage procedures.
The change of duck VBN (TVB-N) in Fig. 5 cold storage procedures.
Embodiment
The principles and features of the present invention are described below, and illustrated embodiment is served only for explaining the present invention, is not intended to
Limit the scope of the present invention.
Embodiment 1：
The preparation of Gorgon fruit shell extract：
1st, prepared by Gorgon fruit shell extract 1
1) Gorgon fruit shell is digested with the aqueous solution of cellulase, the mass ratio of cellulase and Gorgon fruit shell is 1.0%,
Enzymolysis time is 120min, and hydrolysis temperature is 45 DEG C, and enzymolysis liquid pH value is 4.5, obtains digesting mixture；
2) by step 1) obtained enzymolysis mixture directly carries out homogenate extraction, and the extract solution of homogenate extraction is 70% second
Alcohol, the extraction voltage of homogenate extraction is 100V, the extraction time 2min of homogenate extraction, and homogenate extraction is twice；
3) by step 2) obtained extract solution concentrates and is freeze-dried through suction filtration, heating successively, obtains Gorgon fruit shell extract
1。
2nd, prepared by Gorgon fruit shell extract 2
1) Gorgon fruit shell is digested with the aqueous solution of cellulase, the mass ratio of cellulase and Gorgon fruit shell is 1.0%,
Enzymolysis time is 120min, and hydrolysis temperature is 45 DEG C, and enzymolysis liquid pH value is 4.5, obtains digesting mixture；
2) by step 1) obtained enzymolysis mixture directly carries out homogenate extraction, and the extract solution of homogenate extraction is water, flash
The extraction voltage of extraction is 100V, the extraction time 2min of homogenate extraction, and homogenate extraction is twice；
3) by step 2) obtained extract solution concentrates and is freeze-dried through suction filtration, heating successively, obtains Gorgon fruit shell extract
2。
3rd, the drafting of Lu Ding standard curves
The accurate rutin solution for matching somebody with somebody 0.30mg/mL in 50mL volumetric flask.Accurately draw the rutin standard liquid
1.0mL, 2.0mL, 3.0mL, 4.0mL, 5.0mL, 6.0mL, 7.0mL, 8.0mL are respectively placed in 25mL volumetric flasks, each volumetric flask
Middle addition absolute ethyl alcohol is to 12.5mL, then is separately added into 5%NaNO2Aqueous solution 0.7ml, 5min is stood after shaking up；Add 10%
AL(NO3) aqueous solution 0.7mL, shake up, stand 6min；L moL/LNaOH aqueous solution 5mL are separately added into again, add absolute ethyl alcohol
To 25mL, 10min is stood after with absorbance is measured at visible spectrophotometer 510nm, standard curve regression equation is calculated.
4th, in sample flavones content measure
Accurate 1.0mL samples of drawing add absolute ethyl alcohol 11.5mL, shaken up in 25mL volumetric flasks；It is separately added into 5%
NaNO20.7mL, shakes up after placement 5min, adds 10%Al (NO3)3Solution 0.7mL, shakes up, and stands after 6min, adds again respectively
Enter 1mol/L NaOH 5mL, be settled to scale with absolute ethyl alcohol, shake up, stand after 10min, absolute ethyl alcohol does blank, it is ultraviolet can
See survey light absorption value at spectrophotometer 510nm.Substitute into mark song equation and calculate flavones concentration.
Flavones content is flavones content in 26.90%, Gorgon fruit shell water extract in the Gorgon fruit shell alcohol extract extracted by this technique
For 20.88%.
Embodiment 2：
Gorgon fruit shell extract antioxidation activity in vitro is determined
Experiment material：
Sample：The Gorgon fruit shell extract (alcohol extract, water extract) of this bacteriostatic test is prepared by embodiment 1
Reagent：1,1- diphenyl -2- trinitrophenyl-hydrazines (DPPH), 2,2- connection (the 3- ethyls-benzothiazole -6- sulphurs of nitrogen-two
Acid) di-ammonium salts (ABTS), potassium peroxydisulfate, absolute methanol, hydrochloric acid, hydrogen peroxide, chloroform, reduced iron powder, frerrous chloride, sulphur
Potassium cyanate is purchased from Chinese medicines group company.
Equipment：A360 ultraviolet-uisible spectrophotometers, skill of taking wing instrument Shanghai Co., Ltd；Electronic analytical balance, Ao Haosi
International trade Shanghai Co., Ltd；Electric drying oven with forced convection, the permanent Science and Technology Ltd. in Shanghai one.
Test method：
1st, the measure of DPPH free radical scavenging activities
Take extract dissolving adjusted to suitable concn, take 50 μ L solution addition 0.7mL 100 μm of ol/L DPPH molten
In liquid, fully mix, after 30min is kept in dark place in room temperature 517nm at measure absorbance, parallel 3 times, using methanol as blank pair
According to.The ability that extract removes DPPH free radicals is calculated as follows：DPPH free radical scavenging activities (%)=[1- (Ai-Aj)/A0]
×100.In formula, A0 is absorbance of the mixed liquor of 50 μ L methanol and 100 μm of ol/L DPPH solution of 0.7mL at 517nm；
Ai is absorbance of the mixed liquor of 50 μ L samples solution and 100 μm of ol/L DPPH solution of 0.7mL at 517nm；Aj is 50 μ
Absorbance of the mixed liquor of L sample solution and 0.7mL methanol at 517nm.According to the inhibiting rate under series concentration, calculate
Sample concentration when DPPH free radical scavenging activities are 50%, i.e. half-inhibition concentration (IC50) value.
Positive drug：Positive control Vc is taken, its IC50 value is determined as stated above.
2nd, the measure of ABTS radical scavenging activities
In room temperature bar after the 7mmol/L ABTS aqueous solution is mixed with 2.45mmol/L persulfate aqueous solutions (final concentration)
Avoid light place 12-16h under part, forms ABTS+ free radical storing solutions.By this working solution and absolute ethyl alcohol according to 1: 50 (v/v)
Ratio mixing, lower its absorbance for 0.700 ± 0.020 in 734nm, ABTS+ mixed liquors obtained, in pre- hot standby at 30 DEG C
With.After the adjusted content of sample extracting solution to suitable concn, take 50 μ L to add in 1mL ABTS+ mixed liquors and be well mixed, room
The lower lucifuge of temperature stands 30min after surveying its light absorption value under wavelength 734nm, and blank is used as using methanol.Using Trolox as standard items, build
It is vertical be abscissa with Trolox concentration (x, μ g/mL), the standard curve that light absorption value (y) is ordinate, its regression equation is y=-
0.0032x+0.6751, R2=0.9991.Extract is to ABTS radical scavenging activities with contained Trolox in every 100g samples
Equivalent is represented (mg TE/100g FW).Measure is repeated 3 times.
3rd, measure of the Gorgon fruit shell extract to lipid-antioxidant activity activity
Using Schaal Oven Methods, 50g oil samples are taken, are put into 100ml beakers, it is open, by a certain amount of addition Gorgon fruit shell
70% alcohol extract sample liquid, addition is respectively 0.01mg/ml, 0.05mg/ml, 0.1%mg/ml, is well mixed, oil sample is put into
Strengthen in 60 ± 0.5 DEG C of insulating boxs and preserve, every 24h stirrings once, and exchange their positions in insulating box, periodically (0,
2nd, 4,6,8,10,12d) determine the peroxide value (POV) of oil sample.The measure of peroxide value is determined by GB/T5009.37-2003
Colorimetric method.
Experimental result：
Two kinds of extracts have strong DPPH radical scavenging activities it can be seen from table 1, table 2, and IC50 is respectively less than
0.2mg/ml, is approached, Gorgon fruit shell alcohol extract Scavenging activity is better than water extract with positive control Vc IC50 values.Two kinds of extract tools
Have strong ABTS radical scavenging activities, be respectively equivalent to every gram sample in containing water solubility Vc (Trolox) 1.4208 ±
0.0614g、1.4146±0.0065g。
The Gorgon fruit shell extract of table 1 removes DPPH free radical IC50 values
The Gorgon fruit shell extract of table 2 is to ABTS radical scavenging activities
As shown in Figure 1, addition Gorgon fruit shell alcohol extract and BHA rapeseed oil POV values are significantly lower than blank from daystart
Group (P<0.05).The rapeseed oil group POV values for adding 0.1%BHA are significantly lower than other addition groups (P<0.05), it with the addition of
0.01% Gorgon fruit shell alcohol extract, 0.1% Gorgon fruit shell alcohol extract and 0.01%BHA rapeseed oil POV value differences it is different not substantially (P<
0.05).Gorgon fruit shell alcohol extract has certain antioxidation to rapeseed oil, but effect is not so good as 0.1%BHA.
As shown in Figure 2, addition Gorgon fruit shell water extract and BHA rapeseed oil POV values are significantly lower than blank from daystart
Group (P<0.05).The rapeseed oil group POV values for adding 0.1%BHA are significantly lower than other addition groups (P<0.05) 0.1%, is added
The rapeseed oil of Gorgon fruit shell water extract the 5th day the 4th day apparently higher than addition 0.01% Gorgon fruit shell be water extract rapeseed oil
POV values (P<0.05), the 7th day the 6th day then not substantially (P>0.05), with the addition of the rapeseed oil of 0.01% Gorgon fruit shell water extract with
It with the addition of the 0.01%BHA rapeseed oil POV value no significant differences (P before the 6th day>0.05).Gorgon fruit shell water extract is to rapeseed oil
There is certain antioxidation, but effect is not so good as 0.1%BHA.
Embodiment 3
Gorgon fruit shell extract Antibacterial Activity
Experiment material：
Sample：The Gorgon fruit shell extract (alcohol extract, water extract) of this bacteriostatic test is prepared by embodiment 1
For examination strain：Staphylococcus aureus, Escherichia coli, pseudomonad, heat kill rope silk bacterium, corrupt Shiva formula bacterium, breast
Sour bacterium.
Reagent：Nutrient agar, false unit cell selective medium, heat kill rope silk selective medium
Equipment：Finland's Bioscreen fully-automatic analyzers, Shanghai Wei Zai commerce and trade Development Co., Ltd；Whirlpool instrument, Shanghai book
Pretty experimental instruments and equipment limited；The double one side clean work station of SW-CJ-2FD types, Purifying Equipment Co., Ltd., Suzhou；LRH-
100C type low temperature incubators, Shanghai Yiheng Scientific Instruments Co., Ltd；DHP-9082 type electro-heating standing-temperature cultivators, the permanent section in Shanghai one
Learn Instrument Ltd.；DNP-9082 type electro-heating standing-temperature cultivators, the upper grand testing equipment Co., Ltd of Nereid；FSH-2A adjustable heights
Fast refiner, Medical Instruments factory of Jintan City；Portable steam stainless steel sterilizer (autoclave), the limited public affairs of extra large three Shen medicine equipment
Department；CP214 (C) type electronic balance, Ohaus Instrument (Shanghai) Co., Ltd..
Test method：
1st, Bactericidal test
Claim Gorgon fruit shell alcohol extract and water extract powder 2g respectively, two kinds of powder are dissolved in 10mL sterilized water, are configured to
The sample liquid that finite concentration is.
Sterilized agar medium is poured into culture dish, treats that it solidifies per ware 15mL (lower floor)；By sterilized 5mL
Culture medium be cooled to 50 DEG C or so be mixed into 100 μ L test organisms (bacteria suspension concentration be 105—106CFU/mL, experimental bacteria is golden yellow
Grape ball vacation unit cell, Escherichia coli, pseudomonad, hot rope kill silk, corrupt Shiva formula bacterium, and lactic acid bacteria will be mixed with the culture medium of bacterium
It is added to the culture epibasal tier solidified and treats that it solidifies；Oxford cup vertically is put into the media surface solidified, is gently pressurizeed,
It is set to contact tight with culture medium；Sample liquids of the 200 μ L50mg/mL through 0.22 μm of sterile filter processing is added in Oxford cup,
Do not make its excessive, sterilized water does blank；Add and cultivate 12-24h after sample in 37 DEG C or 30 DEG C of incubators, observe result, suppression
Bacterium loop diameter measuring instrument measures diameter.
2nd, minimal inhibitory concentration experimental method
Gorgon fruit each 0.8g of shell extract powder is weighed respectively, and two kinds of powder are dissolved in 2mL solvent to (10%DMSO is molten
Liquid).Dilution (200mg/ml, 100mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml, 6.25mg/ of certain gradient is made
ml、3.125mg/ml).Add each 0.1ml of various concentrations dilution respectively in corresponding MH solid cultures flat board, then add respectively
Plus each bacteria suspension 0.1ml, the completely whole plate of even spread, ware lid is covered, the constant temperature training of preference temperature is inverted in after it is slightly dry
Support in case and cultivate using the minimum sample liquid concentration of not long bacterium as minimal inhibitory concentration.This experiment is drawn relatively using Maxwell turbidimetry
The concentration for answering bacterium is about 105-106Cfu/ml, is tested using the sterilized water containing 10%DMSO as blank control, gathers resistance to propylhomoserin and benzene first
Sour sodium is positive control.Experimental bacteria kills silk, corruption west watt for the false unit cell of Staphylococcus aureus, Escherichia coli, pseudomonad, hot rope
Formula bacterium, lactic acid bacteria), 24-48h is cultivated after being coated with 37 DEG C or 30 DEG C of incubators, result is observed.
3rd, Gorgon fruit shell extract is to cold fresh duck fresh-keeping effect
Gorgon fruit shell alcohol extract, water extract and epsilon-polylysine are dissolved in sterilized water respectively, the sample that 25mg/mL is made is molten
Each 1000mL of liquid.Epsilon-polylysine does positive control, and sterilized water does blank control.
Duck after fresh slaughter is cooled to after segmentation temperature, by after duck well cutting points 4 groups, every group of 200g is used respectively
Controlled atmospheric packing (50%CO after Gorgon fruit shell extract, polylysine and distilled water soak 10 minutes to duck2+ 50%N2) after put 4 DEG C
Refrigerator cold-storage, evaluation index is subjective appreciation, total plate count, T-VBN, every ld (the 0th, 1,3,5,7 days) once referred to
Mapping is determined, and wherein total plate count measures 106No longer determine afterwards.
1) subjective appreciation
Using meat color, epidermis color and luster, smell, elasticity, structural state, water outlet as evaluation index, by GB/T22210-
Special subjective appreciation personnel specified in 2008 carry out sensory evaluation to cold fresh duck.
The organoleptic indicator of the cold fresh duck of table 3
2) total plate count
By GB4789.2-2010《Microbiological test of food hygiene：Total plate count is determined》Method row determines total plate count.
3)T-VBN
VBN (TVB-N values) is determined, specifically by GB/T5009.44-2003《Meat and meat products sanitary standard
Analysis method》Carry out.
Data are calculated：
Experimental result：
The antibacterial circle diameter unit of 3 two kinds of extraction six kinds of bacterium of species of table:mm
Note a：Control group (10%DMSO solution) inhibition zone is 0, unlisted；
Note b：--inhibition zone is not formed.
As can be seen from Table 3, Gorgon fruit shell alcohol extract, water extract to Escherichia coli without fungistatic effect, but to golden yellow grape
Coccus, pseudomonad, Shewanella putrefaciens, heat, which kill rope silk bacterium, obvious fungistatic effect, and its antibacterial circle diameter is in 20~40mm
Between, it is maximum to the antibacterial circle diameter of corrupt western watt Salmonella, it is overall next respectively up to 38.32 ± 0.59 and 34.52 ± 0.63
Say, the fungistatic effect of alcohol extract is better than water extract.
Minimal inhibitory concentration (unit of the 4 two kinds of extracts of table to four kinds of bacterium：mg/mL)
Note：++-represent in 200mg/ml with blank group clump count indifference；
+-represent only have a small amount of bacterium in 200mg/ml.
As can be seen from Table 4, the minimal inhibitory concentration of Gorgon fruit shell alcohol extract will be less than water extract, and alcohol extract is to golden yellow Portugal
The minimal inhibitory concentration of grape coccus is 12.5mg/ml, and concentration is less than positive control epsilon-polylysine, but higher than positive control
Nision.Mlc to corrupt western watt Salmonella is 50mg/ml, is more than 200mg/ml to pseudomonad, effect is weaker than
Positive control polylysine and Nision.
As seen from Figure 3, the sensory evaluation of cold fresh duck is primarily upon its color, smell, elasticity, tissue morphology, with
And whether water outlet.According to sensory evaluation it can be seen that blank group at the 3rd day already below 6 points, had within the 5th day substantially it is different
Taste, and just have slight unhappy to the 5th day Gorgon fruit shell alcohol extract, Gorgon fruit shell water extract group smell, but the 7th day Gorgon fruit shell water is extracted
Group and blank group organoleptic conditions indifference (P>0.05), and Gorgon fruit shell alcohol extract group slightly peculiar smell epsilon-polylysine group then sense organ
Still more than 6 points.
Total plate count is significant for evaluating duck product quality and shelf life, as seen from Figure 4, with storage
The increase of number of days, the increase of duck total plate count.When total plate count is more than 107Cfu/g, illustrates that duck has gone bad, can be with by Fig. 4
See that blank group is rotten at the 4th day, and its total plate count is below after the first day for Gorgon fruit shell alcohol extract, Gorgon fruit shell water extract group
Blank group (P<0.05), higher than positive controls epsilon-polylysine (P<, and Gorgon fruit shell alcohol extract and epsilon-polylysine group 0.05)
Still it is less than 10 in the 7th day its total plate count7cfu/g。
TVB-N values are the important indicators for determining duck product quality and shelf life, as seen from Figure 5 with storage time
Growth, TVB-N values gradually rise.Wherein Gorgon fruit shell alcohol extract, Gorgon fruit shell water extract group are equal in whole storage period TVB-N values
Less than blank group, higher than positive control epsilon-polylysine group (P<0.05).
It may determine that Gorgon fruit shell alcohol extract, Gorgon fruit shell water extract have necessarily fresh-keeping to cold fresh duck by the above results
Effect, two kinds of extracts can extend cold fresh duck shelf life 1~2 day.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (9)
1. a kind of preparation method of Gorgon fruit shell extract, it is characterised in that comprise the following steps：
1) Gorgon fruit shell is digested, obtains digesting mixture；
2) by step 1) obtained enzymolysis mixture directly carries out homogenate extraction, obtains extract solution；
3) by step 2) obtained extract solution concentrates and is freeze-dried through suction filtration, heating successively, obtains Gorgon fruit shell extract.
2. the preparation method of Gorgon fruit shell extract according to claim 1, its feature exists：Step 2) in, homogenate extraction
Extract solution is the ethanol water of water or volume fraction below 80%.
3. the preparation method of Gorgon fruit shell extract according to claim 1 or 2, it is characterised in that step 1) in：With fiber
The aqueous solution of plain enzyme is digested to Gorgon fruit shell, and the mass ratio of cellulase and Gorgon fruit shell is 0.8~1.2%, and enzymolysis time is
90~150min, hydrolysis temperature is 40~50 DEG C, and enzymolysis liquid pH value is 4.0~5.0.
4. the preparation method of Gorgon fruit shell extract according to claim 1 or 2, it is characterised in that step 2) in：It is flash to carry
The extraction voltage taken is 80~120V, and 2~3min of extraction time of homogenate extraction, homogenate extraction is carried out twice.
5. the Gorgon fruit shell that a kind of preparation method according to any described Gorgon fruit shell extract of Claims 1-4 is prepared is carried
Take thing.
6. a kind of application of Gorgon fruit shell extract according to claim 5, it is characterised in that：It is used as antioxidant.
7. the application of Gorgon fruit shell extract according to claim 6, it is characterised in that：For removing DPPH free radicals, it is clear
Except ABTS free radicals.
8. a kind of application of Gorgon fruit shell extract according to claim 5, it is characterised in that：It is used as bacteriostatic agent.
9. the application of Gorgon fruit shell extract according to claim 8, it is characterised in that：For suppressing Staphylococcus aureus
The western watt Salmonella of bacterium, corruption, heat kill rope silk bacterium and/or lactic acid bacteria.
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| CN110419629A (en) * | 2019-04-03 | 2019-11-08 | 江苏大学 | A method of animal and fowl fodder is prepared using Gorgon euryale kind shell and Gorgon fruit bud processing fent extract |
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