CN102783694A - Method for extracting antioxidation active components from euryale seed shell - Google Patents
Method for extracting antioxidation active components from euryale seed shell Download PDFInfo
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- CN102783694A CN102783694A CN2011101849549A CN201110184954A CN102783694A CN 102783694 A CN102783694 A CN 102783694A CN 2011101849549 A CN2011101849549 A CN 2011101849549A CN 201110184954 A CN201110184954 A CN 201110184954A CN 102783694 A CN102783694 A CN 102783694A
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Abstract
The invention relates to a method for extracting antioxidation active components from euryale seed shell. The invention relates to a preparation methods for 4 euryale seed shell extracts and a description of antioxidation activity. According to the invention, euryale seed shell is taken as a raw material, and is subjected to grading extraction by using 4 solvent systems of ethyl acetate, 95% ethanol, 50% ethanol and water, the extract is subjected to pressure reduction and concentration, and is subjected to freeze drying for 48 hours to obtain 4 euryale seed shell extracts: A, B, C and D. The obtained extracts are in a powdery state, the colors are light yellow, shallow brown, brown and dark brown in order, no peculiar smell is generated; and the total phenol content are 537.23 mg/g, 563.65 mg/g, 557.04 mg/g and 177.62mg/g respectively. Compared with a vitamin C solution with same concentration, four extracts display strong reducing power, the DPPH. clearance rate and anti-lipid peroxide capability, intermediate strength active oxygen (O2<->.) clearance rate and NO2<-> clearance activity, and has certain exploitation potential in the food anti-oxidant field.
Description
Technical field:
The preparation method and the antioxidation activity thereof that the present invention relates to 4 kinds of Gorgon euryale kind shell extracts are described.
Background technology:
Gorgon fruit is the mature seed kernel of a kind of water plant Gorgon euryale, is commonly called as Gorgon euryale rice, is one of China's traditional Chinese medicine material, also is one of " dietotherapeutic " material of the first batch of promulgation of China Ministry of Public Health.Gorgon fruit has plurality of health care functions, has " tonifying spleen, dampness removing, reinforce the kidney " effect (" book on Chinese herbal medicine is looked for the truth ").In China, Gorgon fruit is distributed in ground such as Zhejiang, Jiangsu, Anhui, Guangdong, Shandong, Hubei more.Produce about 10000 tons in dried Gorgon euryale rice (2010) per year.
The Gorgon fruit seed is outer by wooden kind of thick shell of one deck 1-2mm, and therefore, shelling is the first step of Semen Euryales processing.Gorgon euryale kind chitin amount accounts for the 30%-40% of Gorgon fruit seed gross mass, is the main accessory substance in the Gorgon fruit process, also untapped at present utilization.
Summary of the invention:
The present invention is a raw material with Gorgon euryale kind shell, and after pulverizing (certain grain size), the dicyandiamide solution that adopts 4 kinds of polarity such as ethyl acetate, 95% ethanol, 50% ethanol, water to increase successively again is down auxiliary at ultrasonic wave; Gorgon euryale kind shell (powder) is carried out successively grading extraction, and (be that same raw material extracts with 4 kinds of solvents successively, every kind of solvent extracts 3 times continuously, the centrifugation of slag liquid; The extract that merges same solvent); After the extract decompression concentrated, freeze drying 48h promptly obtained pulverous 4 kinds of extract: A, B, C and D.4 kinds of Gorgon euryale kinds of gained shell extract all is Powdered, and metallic luster is arranged, and that color is followed successively by is faint yellow, light brown, brown and dark brown, and free from extraneous odour has different dissolubilities in water.
Total phenol content is respectively 537.23,763.65,557.04 and 177.62mg/g in A, B, C and 4 kinds of Gorgon euryale kinds of the D shell extract, extract total yield 37.91%.Compare with the vitamin c solution of same concentrations, these 4 kinds of extracts all show stronger reducing power and remove DPPH and suppress the lipid peroxidation activity, show the active oxygen (O of moderate strength
2 -) and NO
2 -Remove activity, aspect food antioxidant, have certain potentiality to be exploited.
The present invention has following characteristics and innovation:
1, as a kind of comprehensive utilization of local characteristic resource, the present invention benefits our pursuits in the application in functional food additives field to Gorgon euryale kind shell resource, and has obtained comparatively ideal results.
2, be rich in the plant polyphenol material in 4 kinds of Gorgon euryale kind shell extracts that adopt the present invention to prepare, antioxidation activity is remarkable, and free from extraneous odour has certain potentiality to be exploited aspect food antioxidant.
3, adopt the present invention to prepare 4 kinds of Gorgon euryale kind shell extracts, extraction process is simple, and slag liquid is easy to separate, and it is nontoxic to extract solvent, and recyclable back reuses, and meets the management philosophy of modern enterprise low energy consumption, low emission and environmental protection.
4,4 kinds of Gorgon euryale kind shell extracts of the present invention's preparation adopt ultrasonic-assisted extraction technology, can under low temperature (10-20 ℃), improve sample extraction rate (37.91%) greatly, cut down the consumption of energy.In addition, end temperature ultrasonic-assisted extraction technology can shorten extraction time greatly, has improved extraction efficiency.
Figure of description is main preparation technology's flow chart of the present invention's (promptly the preparation method of 4 kinds of Gorgon euryale kind shell extracts and antioxidation activity thereof are described).
The practical implementation flow process:
Below,, the practical implementation process of content of the present invention is described in conjunction with its antioxidation activity analysis result through Gorgon euryale kind shell extract preparation technology flow chart:
(1) raw material: when producing Gorgon euryale kind shell per year, (crossing the 40-60 eye mesh screen) pulverized in 40 ℃ of oven dry down before using, obtain Gorgon euryale kind shell powder.
(2) the ultrasonic wave secondary solvent extracts:
Dicyandiamide solution: ethyl acetate, 95% ethanol, 50% ethanol, water.
Extract: take by weighing a certain amount of Gorgon euryale kind shell powder, add the above-mentioned solvent of 10 times of amounts (v/w) successively, (every kind of solvent extracts 3 times (10-20 ℃) ultrasonic-assisted extraction under the normal temperature continuously; 10min/ time); The centrifugation of slag liquid, supernatant merges, after decompression concentrates and reclaims solvent; Freeze drying 48h promptly obtains 4 kinds of Gorgon euryale kind shell extracts: A, B, C and D.
(3) 4 kinds of Gorgon euryale kinds of gained shell extract physicochemical property and antioxidation activity analysis.
Analysis of physical and chemical property comprises: total phenol, total sugar content analysis, uv absorption character etc.;
The antioxidation activity analytical method comprises: total reducing power, DPPH clearance rate, O
2 -Clearance rate, NO
2 -5 kinds of antioxidation activity in vitro analytical methods such as removing ability and anti peroxidation of lipid ability.
Claims (6)
1. the extraction and the preparation method of antioxidation activity component in the Gorgon euryale kind shell, content comprises the extraction preprocessing method of raw materials, extracts dicyandiamide solution and forms and extract flow process, extract preparation technology etc.
2. antioxidation activity method for preparing ingredients thereof in the Gorgon euryale kind shell as claimed in claim 1 obtains 4 kinds of extracts.Adopt total reducing power, DPPH clearance rate, O respectively
2 -Clearance rate, NO
2 -5 kinds of antioxidation in vitro property analytical methods such as removing ability and anti peroxidation of lipid ability are analyzed the antioxidation activity of above-mentioned 4 kinds of Gorgon euryale kind shell extracts, and this patent is described its antioxidation activity result.
3. 4 kinds of Gorgon euryale kind shell extracts as claimed in claim 1 are to be raw material with Gorgon euryale kind shell, and after pulverizing (certain grain size), the dicyandiamide solution that adopts 4 kinds of polarity to increase successively again is down auxiliary at ultrasonic wave; Gorgon euryale kind shell (powder) is carried out successively grading extraction, and (be that same raw material extracts with 4 kinds of solvents successively, every kind of solvent extracts 3 times continuously, the centrifugation of slag liquid; The extract that merges same solvent); After the extract decompression concentrated, freeze drying 48h promptly obtained pulverous 4 kinds of extract: A, B, C and D.
4. like claim 1,2 or 3 described 4 kinds of Gorgon euryale kind shell extracts: A, B, C and D, wherein total phenol content is respectively 537.23,763.65,557.04 and 177.62mg/g, extract total yield 37.91%.These 4 kinds of extracts show all that than strong reducing property in 10-50 μ g/mL concentration range, its total reducing power all is higher than or approaches the V of same concentrations
CExtract demonstrates very strong DPPH scavenging action when low concentration, when 0.1mg/mL, its DPPH clearance rate is all above 80%.In addition, Gorgon euryale kind shell extract to the lipid peroxidation inhibiting rate all up to 90% (0.5mg/mL, 12h).Show that these 4 kinds of Gorgon euryale kind shell extracts have stronger reducing power and remove DPPH and suppress the lipid peroxidation activity, show the active oxygen (O of moderate strength
2 -) and NO
2 -Remove active.
5. like claim 1,2 or 3 described 4 kinds of Gorgon euryale kind shell extracts: A, B, C and D, all be Powdered, metallic luster is arranged, that color is followed successively by is faint yellow, light brown, brown and dark brown, and free from extraneous odour has different dissolubilities in water.
6. like claim 1,2,3 and 4 described 4 kinds of Gorgon euryale kind shell extracts: A, B, C and D, have stronger antioxidation activity and remove the free radical ability, aspect food antioxidant, have certain potentiality to be exploited.
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| CN2011101849549A CN102783694A (en) | 2011-06-24 | 2011-06-24 | Method for extracting antioxidation active components from euryale seed shell |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103638113A (en) * | 2013-11-28 | 2014-03-19 | 江苏大学 | Method for preparing antibiotic substance from gordon euryale processing waste liquor (deastringent liquor) |
| CN104027397A (en) * | 2014-03-25 | 2014-09-10 | 江苏大学 | Preparation method and application of anti-allergic agent from both medicinal and edible plant extract |
| CN106317241A (en) * | 2016-08-26 | 2017-01-11 | 滁州学院 | Method for separating and purifying polysaccharide in gorgon fruit peels and product thereof |
| CN107242564A (en) * | 2017-05-25 | 2017-10-13 | 武汉轻工大学 | Gorgon fruit shell extract and its preparation method and application |
| CN110090243A (en) * | 2019-05-21 | 2019-08-06 | 江西农业大学 | A kind of Semen euryales extract for Postprandial glucose control, preparation method and application |
| CN110419629A (en) * | 2019-04-03 | 2019-11-08 | 江苏大学 | A method of animal and fowl fodder is prepared using Gorgon euryale kind shell and Gorgon fruit bud processing fent extract |
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| CN101803777A (en) * | 2010-04-09 | 2010-08-18 | 江南大学 | Aquatic fruit and vegetable juice anti-oxidation composite beverage and preparation method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103638113A (en) * | 2013-11-28 | 2014-03-19 | 江苏大学 | Method for preparing antibiotic substance from gordon euryale processing waste liquor (deastringent liquor) |
| CN103638113B (en) * | 2013-11-28 | 2016-04-06 | 江苏大学 | A kind of method preparing antibiotic substance from Semen Euryales processing-waste (remove astringent taste liquid) |
| CN104027397A (en) * | 2014-03-25 | 2014-09-10 | 江苏大学 | Preparation method and application of anti-allergic agent from both medicinal and edible plant extract |
| CN104027397B (en) * | 2014-03-25 | 2016-04-06 | 江苏大学 | A kind of preparation method of integration of edible and medicinal herbs plant extract anti-sensitizer and application |
| CN106317241A (en) * | 2016-08-26 | 2017-01-11 | 滁州学院 | Method for separating and purifying polysaccharide in gorgon fruit peels and product thereof |
| CN106317241B (en) * | 2016-08-26 | 2018-08-14 | 滁州学院 | The isolation and purification method and products thereof of polysaccharide in a kind of Gorgon euryale pericarp |
| CN107242564A (en) * | 2017-05-25 | 2017-10-13 | 武汉轻工大学 | Gorgon fruit shell extract and its preparation method and application |
| CN110419629A (en) * | 2019-04-03 | 2019-11-08 | 江苏大学 | A method of animal and fowl fodder is prepared using Gorgon euryale kind shell and Gorgon fruit bud processing fent extract |
| CN110090243A (en) * | 2019-05-21 | 2019-08-06 | 江西农业大学 | A kind of Semen euryales extract for Postprandial glucose control, preparation method and application |
| CN110090243B (en) * | 2019-05-21 | 2021-07-30 | 江西农业大学 | Gorgon fruit extract for controlling postprandial blood sugar, preparation method and application |
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Application publication date: 20121121 |