CN107242072A - Selenium-enriched vine tea and preparation method thereof - Google Patents
Selenium-enriched vine tea and preparation method thereof Download PDFInfo
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- CN107242072A CN107242072A CN201710414780.8A CN201710414780A CN107242072A CN 107242072 A CN107242072 A CN 107242072A CN 201710414780 A CN201710414780 A CN 201710414780A CN 107242072 A CN107242072 A CN 107242072A
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- 241001122767 Theaceae Species 0.000 title claims abstract description 156
- 239000011669 selenium Substances 0.000 title claims abstract description 115
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 94
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 93
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000003337 fertilizer Substances 0.000 claims abstract description 98
- 239000002689 soil Substances 0.000 claims abstract description 73
- 235000016709 nutrition Nutrition 0.000 claims abstract description 68
- 230000035764 nutrition Effects 0.000 claims abstract description 67
- 238000000855 fermentation Methods 0.000 claims abstract description 55
- 230000004151 fermentation Effects 0.000 claims abstract description 55
- 150000001875 compounds Chemical class 0.000 claims abstract description 53
- 241000196324 Embryophyta Species 0.000 claims abstract description 38
- 230000000813 microbial effect Effects 0.000 claims abstract description 32
- 238000001035 drying Methods 0.000 claims abstract description 22
- 238000010009 beating Methods 0.000 claims abstract description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 27
- 235000009566 rice Nutrition 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 235000019354 vermiculite Nutrition 0.000 claims description 22
- 229910052902 vermiculite Inorganic materials 0.000 claims description 21
- 239000010455 vermiculite Substances 0.000 claims description 21
- 238000003756 stirring Methods 0.000 claims description 20
- 241000287828 Gallus gallus Species 0.000 claims description 14
- 235000004443 Ricinus communis Nutrition 0.000 claims description 14
- 244000057717 Streptococcus lactis Species 0.000 claims description 14
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 14
- 210000003608 fece Anatomy 0.000 claims description 14
- 239000010871 livestock manure Substances 0.000 claims description 14
- 235000012054 meals Nutrition 0.000 claims description 14
- 244000068988 Glycine max Species 0.000 claims description 13
- 235000010469 Glycine max Nutrition 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 12
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 12
- 239000004202 carbamide Substances 0.000 claims description 12
- 238000003306 harvesting Methods 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid group Chemical class S(O)(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 7
- 239000003463 adsorbent Substances 0.000 claims description 7
- 239000004021 humic acid Substances 0.000 claims description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 239000011701 zinc Substances 0.000 claims description 4
- 229910052725 zinc Inorganic materials 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 4
- 241000545744 Hirudinea Species 0.000 claims 2
- 239000001506 calcium phosphate Substances 0.000 claims 2
- 235000011010 calcium phosphates Nutrition 0.000 claims 2
- 235000013312 flour Nutrition 0.000 claims 2
- 239000004575 stone Substances 0.000 claims 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims 2
- 238000011160 research Methods 0.000 abstract description 9
- 238000012545 processing Methods 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 206010033546 Pallor Diseases 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract 1
- 229940091258 selenium supplement Drugs 0.000 description 79
- 241000209094 Oryza Species 0.000 description 23
- 238000005096 rolling process Methods 0.000 description 17
- 238000004898 kneading Methods 0.000 description 16
- 241000894006 Bacteria Species 0.000 description 10
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 10
- 235000019691 monocalcium phosphate Nutrition 0.000 description 10
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 9
- 240000003553 Leptospermum scoparium Species 0.000 description 9
- 235000015459 Lycium barbarum Nutrition 0.000 description 9
- 239000002131 composite material Substances 0.000 description 9
- 238000002386 leaching Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 238000001816 cooling Methods 0.000 description 8
- 238000011161 development Methods 0.000 description 8
- ILRLTAZWFOQHRT-UHFFFAOYSA-N potassium;sulfuric acid Chemical compound [K].OS(O)(=O)=O ILRLTAZWFOQHRT-UHFFFAOYSA-N 0.000 description 8
- 238000003892 spreading Methods 0.000 description 8
- WQSRXNAKUYIVET-UHFFFAOYSA-N sulfuric acid;zinc Chemical compound [Zn].OS(O)(=O)=O WQSRXNAKUYIVET-UHFFFAOYSA-N 0.000 description 8
- 230000005540 biological transmission Effects 0.000 description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 description 4
- 235000013824 polyphenols Nutrition 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 235000014347 soups Nutrition 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000219099 Parthenocissus quinquefolia Species 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 241000345998 Calamus manan Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 235000009388 Parthenocissus quinquefolia Nutrition 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 235000012950 rattan cane Nutrition 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- 240000002900 Arthrospira platensis Species 0.000 description 1
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 240000005674 Ceanothus americanus Species 0.000 description 1
- 235000014224 Ceanothus americanus Nutrition 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001018563 Nekemias grossedentata Species 0.000 description 1
- 241000208966 Polygala Species 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- PWPJGUXAGUPAHP-UHFFFAOYSA-N lufenuron Chemical compound C1=C(Cl)C(OC(F)(F)C(C(F)(F)F)F)=CC(Cl)=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F PWPJGUXAGUPAHP-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229940082569 selenite Drugs 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-L selenite(2-) Chemical compound [O-][Se]([O-])=O MCAHWIHFGHIESP-UHFFFAOYSA-L 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- -1 therefore Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of selenium-enriched vine tea and preparation method thereof, the preparation method comprises the following steps：(1) microbial fermentation fertilizer is prepared；(2) nutrition compound fertilizer is prepared；(3) apply fertilizer；(4) it is colonized；(5) finish；(6) knead, be drying to obtain.Selenium-enriched vine tea of the present invention and preparation method thereof, influence of the research fertilizer application condition to vine tea main components and active component is sought to obtain the vine tea fertilizer application condition of high-quality and high Se content；The administration of nutrition compound fertilizer can not only provide plant corresponding nutrient, while can improve soil physical and chemical property, promote absorbing for soil nutrient, thus promote plant grow and quality improvement；And study the optimal processing parameter of vine tea fixing, with fixation time, throw influence of the different fixing mode of research microwave de-enzyming, blanching fixing, three kinds of the steam beating such as leaf amount, fixing processing mode to vine tea quality, it is ensured that final the absorbing by human body of selenium-enriched vine tea nutritional ingredient.
Description
Technical field
The present invention relates to the preparation method of vine tea, more particularly to a kind of selenium-enriched vine tea and preparation method thereof.
Background technology
Natural Se rich tea is mainly produced from the higher area of soil Se content, and the selenium content of soil has with strata structure
Close relationship, content of selenium in tea is relevant also with the kind of tea tree in addition.And most area can only by soil application or
Foliage-spray selenate or selenite reach the purpose of selenium-rich, there are some researches show, foliage-spray sodium selenite by young leaves and
Bud is absorbed into Organic Selenium, and the quality of Se content and improvement tealeaves to tealeaves has obvious facilitation, the richness of production
The chemical nature of selenium tea and natural Se rich tea is identical.
There are enshi, Ziyang, shaanxi, Anhui Shitai County and its Zhongxin of Kaiyang, Guizhou, Sichuan Wanyuan, river in the high selenium area of Chinese soil
The part such as Xifeng city tea area, these tea areas form Chinese Se rich tea grown place band, and content of selenium in tea elsewhere is general very low.
Research confirms that the Enshitujianationalitymiaonationality Autonomous Prefecture in the Hubei west and south is the high selenium zone of Largest In China, and soil selenium-containing amount reaches 20-
30mg/kg, high reachable 40mg/kg, its selenium content are 1682 times of earth's crust selenium Clarke value.Tea Se content of bestowing favour reaches a lot
More than 9-30 times of area.Ziyang County soil selenium-containing amount is 0.209-26.35mg/kg, content of selenium in tea 0.122-3.854mg/
Kg is higher 4.5-19 times than other domestic southern tea area tealeaves Se contents.Zhongxin of Kaiyang, Guizhou soil selenium-containing amount scope is in 0.175-
3.00mg/kg, average 0.61mg/kg, far above average national level 0.290mg/kg, have developed a variety of Se rich tea productions
Product.In the case where not applying selenium fertilizer, the selenium in tealeaves is mainly derived from soil, and sand helps qin etc. to soil available selenium and soil class
The correlation of type and fresh leaf selenium content is studied, and is as a result shown：Soil available Se content and tealeaves Se content are significantly correlated,
It is to influence one of principal element of content of selenium in tea；And same kind is also different in the selenium content of Different Soil, sequentially it is：
Red-yellow soil>Lateritic red loam>Red soil>Acid purple soil>Moisture soil>Cinnamon soil ＞ brunisolic soils.Fordyce etc. is by difference of bestowing favour
The sampling study of regional selenium shows that the bioavailability of the regional selenium of high selenium is gone back and soil pH except relevant with the total Se content of soil
It is related.
Selenium has different physiological roles in human body, including improves the activity, strengthen immunity, protection painstaking effort of antioxidase
Pipe function, promotion growth, the protection organs of vision, removing toxic substances, protection liver, anti-aging and anti-cancer etc..Enrichment master of the selenium in food
The assimilation of plant, animal or microorganism to inorganic selenium is relied on, because ordinary food selenium content is relatively low, by eating various kinds of foods
Human body selenium intake can not be met.Domestic and foreign scholars are sought for a kind of high Se content, bioactivity is higher but cytotoxicity is low
Compound and plant.China begins through the reinforcing salt of inorganic selenium to improve the intake of human body selenium, 90 years from the eighties
For the research for starting selenium-enriched plant initial stage, to improve the healthcare function of plant.From selenium-rich rice, selenium-rich soybean, selenium enriched Spirulina,
Se-rich lucid ganoderma, product containing organic selenium and selenium-enriched tea leaf etc., the quality of plant significantly improve, and selenium content significantly increases.Therefore, seek
It is very important as selenium-rich carrier efficiently with safe plant to ask a kind of.
The content of the invention
In view of many shortcomings of the prior art, the present inventor passes through substantial amounts of further investigation, paying sufficiently
There is provided a kind of selenium-enriched vine tea and preparation method thereof after creative work.
The present invention seeks to what is be achieved through the following technical solutions：
A kind of preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 50-60 parts by weight of soybean dregs of rice, 10-20 parts by weight Castor seed Meal, 0.8-1.8 parts by weight streptococcus lactis, 0.4-
Fermented after 0.9 parts by weight S. cervisiae, 10-20 parts by weight water are well mixed, obtain microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 125-135 parts by weight chicken manure, 155-165 parts by weight powdered rice hulls, 15-25 weight
Part calcium superphosphate, 5-15 parts by weight of urea, 15-25 parts sulfuric acids potassium, 1-3 parts sulfuric acids zinc, 3-9 parts by weight microorganism inhale
Attached dose is well mixed, and obtains nutrition compound fertilizer；
(3) nutrition compound fertilizer 1000-3000kg is applied in every mu of soil；
(4) vine tea nursery stock is colonized；
(5) the vine tea tender leaf after harvesting is colonized 2 years is finished；
(6) kneaded, be drying to obtain after finishing.
A kind of preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 50-60 parts by weight of soybean dregs of rice, 10-20 parts by weight Castor seed Meal, 0.8-1.8 parts by weight streptococcus lactis, 0.4-
Fermented after 0.9 parts by weight S. cervisiae, 10-20 parts by weight water are well mixed, obtain microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 125-135 parts by weight chicken manure, 155-165 parts by weight powdered rice hulls, 15-25 weight
Part calcium superphosphate, 5-15 parts by weight of urea, 15-25 parts sulfuric acids potassium, 1-3 parts sulfuric acids zinc, 3-9 parts by weight microorganism inhale
Attached dose is well mixed, and obtains nutrition compound fertilizer；
(3) nutrition compound fertilizer 1000-3000kg is applied in every mu of soil, ploughs deeply 20-30 centimetres of ground, and the soil for rolling up is struck
It is broken to be well mixed with nutrition compound fertilizer；
(4) vine tea nursery stock is colonized, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often mu plant 220-
320 plants, annual March, August respectively apply nutrition compound fertilizer once after field planting, apply nutrition compound fertilizer 1000- for every mu every time
2000kg；
(5) the vine tea tender leaf after harvesting is colonized 2 years is finished；
(6) kneaded, be drying to obtain after finishing.
Preferably, the temperature of the fermentation is 25-35 DEG C, and pH is 4-6.5, and fermentation time is companion in 2-4 days, fermentation process
There is stirring, stir speed (S.S.) is 20-80 revs/min.
Preferably, the microbial adsorbent is mixture one or more in vermiculite power, humic acid, turf.
It is further preferred that the microbial adsorbent is PERFORMANCE OF MODIFIED VERMICULITE powder.
The PERFORMANCE OF MODIFIED VERMICULITE powder is prepared by following methods：By 0.3-0.8mol/L ammonium sulfates and vermiculite power in mass ratio
For 1：5 mixing, immersion is centrifuged after 12-36 hours, drying, as PERFORMANCE OF MODIFIED VERMICULITE powder.
It is highly preferred that the microbial adsorbent is made up of 75-85wt% PERFORMANCE OF MODIFIED VERMICULITEs powder and 15-25wt% humic acid.
Preferably, the fixing is frying fixing, microwave de-enzyming or steam beating.
It is further preferred that the fixing is microwave de-enzyming.
Preferably, it in temperature is 90-110 DEG C that the drying, which is,.
Present invention also offers a kind of selenium-enriched vine tea, it is prepared from using the above method.
Plant mainly obtains in the various nutrients needed for growth and development process from soil, therefore, soil nutrient shape
Condition is directly connected to the yield and quality of crop, is highly important environmental condition.Measures of fertilizer is to soil physical property, nutrition
Supply and have considerable influence to crop quality, yield.
With to vine tea Study on functional properties ground deep, the further reinforcement to vine tea product development, wild vine tea resource
The demand of people far can not be met.Vine tea artificial cultivation is imperative, how to improve vine tea yield and matter by measures of fertilizer
Amount, the content for especially increasing vine tea active component has important practical significance.And related research is still a blank.
Selenium-enriched vine tea of the present invention and preparation method thereof, research fertilizer application condition is to vine tea main components and active component
Influence, seeks to obtain the vine tea fertilizer application condition of high-quality and high Se content；The administration of nutrition compound fertilizer can not only provide plant
The corresponding nutrient of thing, while can improve soil physical and chemical property, promotes absorbing for soil nutrient, so as to promote the life of plant
Long development and the improvement of quality；And the optimal processing parameter of vine tea fixing is studied, it is micro- to throw the researchs such as leaf amount, fixing processing mode
Influence of the different fixing mode of ripple fixing, blanching fixing, three kinds of steam beating to vine tea quality, it is ensured that selenium-enriched vine tea nutrition
Final the absorbing by human body of composition.
Embodiment
Selenium-enriched vine tea polyphenol content is determined：With reference to GB/T 8313-2008《Tea Polyphenols in Tea and catechin content
Detection method》.
The measure of selenium-enriched vine tea soup look：Take 2 grams of selenium-enriched vine tea hot water constant volumes to 100mL, filter, take while hot after 5 minutes
Filtrate, separately adds 1mL95% ethanol with suction pipe, is then shaken up to 25mL with deionized water constant volume, surveys its suction at 420nm
Shading value.
The total Se content of selenium-enriched vine tea and selenium leaching rate are determined：With reference to Chen Yongbo etc.《In Se rich tea the leaching rate of selenium and effectively
Property evaluate》, selenium-enriched vine tea sample 1g is weighed in small beaker, with the ultrapure flooding boiled 3 times, add water 100ml every time, extraction
Time is 10min, respectively does 2 parallel, 3 leaching liquors of merging, determines total Se content, selenium in the Se content and tealeaves in leaching liquor
Leaching rate (%)=(Se content × leaching liquor gross weight in leaching liquor)/(total Se content of selenium-enriched vine tea × selenium-enriched vine tea gross weight
Amount) × 100%.
Streptococcus lactis in embodiment, strain number：ACCC10653, latin name：Streptococcus Lactis, viable bacteria
Number：500000000/ml.
S. cervisiae in embodiment, strain number：ACCC10619, latin name：Saccharomyces
Cerevisiae, viable bacteria number：0.6 hundred million/ml.
Soybean Meal in embodiment, the Soybean Meal provided using beneficial extra large (Lianyun Harbour) cereal and oil industry Co., Ltd
Castor seed Meal in embodiment, the Castor seed Meal provided using the big Haitai castor-oil plant Co., Ltd in Shandong Zouping south.
Chicken manure in embodiment, the chicken manure provided using Shijiazhuang Lv Yunfei industry Co., Ltd.
Powdered rice hulls in embodiment, the 60 mesh powdered rice hulls provided using Lingshou County Hua Chen Mineral Products Trades Co., Ltd.
Vermiculite power in embodiment, the 80 mesh vermiculite powers provided using mineral products processing factory of Lingshou County Cigna.
Humic acid in embodiment, the 80 mesh humic acid provided using Jiangxi polygala humic acid Co., Ltd.
Vine tea is commonly called as Doanngo tea, rattan mother-in-law tea (also known as mountain Sweet tea, dragon beard tea), botanical character：It is Vitaceae Ampelopsis
The young stem and leaf of ampelopsis grossdentata section (Ampelopsis grossedentata).English name is Vine tea.Rattan in embodiment
Tea shoot wood is robust growth (without withered branch and sick branch), highly well developed root system, the annual apogamic seedling for 20cm.
Embodiment 1
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc are well mixed, and obtain nutrition compound fertilizer；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is microwave de-enzyming, is adopted
Finished with 6CW-10E type tea microwave de-enzymings drying equipment (Nanjing Agriculturing Mechanization Inst., Ministry of Agriculture's development), technique
Condition：Microwave power is 5 × 2.55kW, and transmission speed is 550 revs/min, throws leaf amount 100g；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.
Embodiment 2
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc, 5 parts by weight vermiculite powers are well mixed, and are obtained nutrition and are combined
Fertilizer；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is microwave de-enzyming, is adopted
Finished with 6CW-10E type tea microwave de-enzymings drying equipment (Nanjing Agriculturing Mechanization Inst., Ministry of Agriculture's development), technique
Condition：Microwave power is 5 × 2.55kW, and transmission speed is 550 revs/min, throws leaf amount 100g；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.
Embodiment 3
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc, 5 parts by weight vermiculite powers are well mixed, and are obtained nutrition and are combined
Fertilizer；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is to be in temperature
Frying 7 minutes in 120 DEG C of iron pan；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.
Embodiment 4
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc, 5 parts by weight vermiculite powers are well mixed, and are obtained nutrition and are combined
Fertilizer；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is steam beating, is killed
The blue or green 3.5 minutes time, throw leaf amount 100g；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.
Embodiment 5
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc, 5 part by weight modified vermiculite powers are well mixed, and obtain nutrition
Compound fertilizer；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is microwave de-enzyming, is adopted
Finished with 6CW-10E type tea microwave de-enzymings drying equipment (Nanjing Agriculturing Mechanization Inst., Ministry of Agriculture's development), technique
Condition：Microwave power is 5 × 2.55kW, and transmission speed is 550 revs/min, throws leaf amount 100g；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.
The PERFORMANCE OF MODIFIED VERMICULITE powder is prepared by following methods：By 0.6mol/L ammonium sulfates (i.e. ammonium sulfate solution, wherein
The molar concentration of ammonium sulfate is 0.6mol/L) it is 1 in mass ratio with vermiculite power：5 mixing, using rotating speed as 5000 after soaking 25 hours
Rev/min centrifugation 10 minutes, take be deposited at 90 DEG C dry 24 hours, obtain PERFORMANCE OF MODIFIED VERMICULITE powder.
Embodiment 6
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc, 5 parts by weight humic acid are well mixed, and are obtained nutrition and are combined
Fertilizer；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is microwave de-enzyming, is adopted
Finished with 6CW-10E type tea microwave de-enzymings drying equipment (Nanjing Agriculturing Mechanization Inst., Ministry of Agriculture's development), technique
Condition：Microwave power is 5 × 2.55kW, and transmission speed is 550 revs/min, throws leaf amount 100g；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.
Embodiment 7
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc, 5 parts by weight turfs are well mixed, and obtain nutrition composite fertilizer
Material；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is microwave de-enzyming, is adopted
Finished with 6CW-10E type tea microwave de-enzymings drying equipment (Nanjing Agriculturing Mechanization Inst., Ministry of Agriculture's development), technique
Condition：Microwave power is 5 × 2.55kW, and transmission speed is 550 revs/min, throws leaf amount 100g；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.
Embodiment 8
The preparation method of selenium-enriched vine tea, comprises the following steps：
(1) by the 55 parts by weight of soybean dregs of rice, 15 parts by weight Castor seed Meals, 1.4 parts by weight streptococcus lactis, 0.65 parts by weight wine brewing ferment
Seal, fermented after female bacterium, 15 parts by weight water are well mixed, the temperature of fermentation is 32 DEG C, and pH is 5, fermentation time is 3 days,
With stirring in fermentation process, stir speed (S.S.) is 30 revs/min, obtains microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 130 parts by weight chicken manures, 160 parts by weight powdered rice hulls, 20 parts by weight calcium superphosphate,
10 parts by weight of urea, 20 parts sulfuric acid potassium, 2 parts sulfuric acid zinc, 4 part by weight modified vermiculite powers, the mixing of 1 parts by weight humic acid
Uniformly, nutrition compound fertilizer is obtained；
(3) select soil acidity or alkalinity 5.5 or so in enshi the Tujia and Miao nationalities autonomous prefecture Xuanen County, humidity is preferable
Area；Nutrition compound fertilizer 2000kg is applied in every mu of soil, ploughs deeply 25 centimetres of ground, and the soil for rolling up is broken into pieces and nutrition composite fertilizer
Material is well mixed；
(4) vine tea nursery stock is colonized in mid-April, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often
260 plants of mu plant, annual March, August respectively apply nutrition compound fertilizer once after field planting, and nutrition compound fertilizer is applied in every mu of soil
1200kg, ploughs deeply 15 centimetres of ground every time, and the soil for rolling up is broken into pieces and is well mixed with nutrition compound fertilizer；As the high 45cm of treelet
Terminal bud is extractd, promotes side shoot to sprout and the voluminous leaf of plant dwarfing, and easily pick tea-leaves；It is to pluck intermediately remained border to pluck top principle, plucks and forces to stay on weak, rush
Enter that tea is fluffy to be formed early, tea tree height control is advisable in below 1m；
(5) the vine tea tender leaf grown at the beginning of 5 months after harvesting is colonized 2 years is finished, and the fixing is microwave de-enzyming, is adopted
Finished with 6CW-10E type tea microwave de-enzymings drying equipment (Nanjing Agriculturing Mechanization Inst., Ministry of Agriculture's development), technique
Condition：Microwave power is 5 × 2.55kW, and transmission speed is 550 revs/min, throws leaf amount 100g；
(6) by the vine tea tender leaf spreading for cooling after fixing to room temperature, with 6CR-30 types tea twisting machine, (ocean machinery is preferential on Zhejiang
Company) kneaded, according to the principle of " light-weight-light ", it is not pressurized and kneads, light kneadding is twisted with the fingers, weight is kneaded, decompress, lower machine；Will
Vine tea tender leaf after kneading is put into air dry oven and dried, and drying temperature is 105 DEG C, is taken out once every 15 minutes, in room
Spread under temperature 10 minutes.Dry and spread alternately, until the vine tea water content in tender leaf after kneading produces selenium-rich to 6.5%
Vine tea.Selenium-enriched vine tea test result：Polyphenol content 35.7mg/g, total Se content is 2.81mg/kg, and soup look is 0.304, selenium leaching
Extracting rate is 29.6%.
The PERFORMANCE OF MODIFIED VERMICULITE powder is prepared by following methods：By 0.6mol/L ammonium sulfates (i.e. ammonium sulfate solution, wherein
The molar concentration of ammonium sulfate is 0.6mol/L) it is 1 in mass ratio with vermiculite power：5 mixing, using rotating speed as 5000 after soaking 25 hours
Rev/min centrifugation 10 minutes, take be deposited at 90 DEG C dry 24 hours, obtain PERFORMANCE OF MODIFIED VERMICULITE powder.
Test case 1
Polyphenol content, soup look, total Determination of Selenium are carried out to selenium-enriched vine tea prepared by embodiment 1-2 and 5-7, to implementing
Selenium-enriched vine tea prepared by example 2-4 carries out soup look, total Se content, selenium leaching rate and determined, and specific test result is shown in Table 1.
Table 1：Test result table
The present invention is by studying the optimal processing parameter that vine tea finishes, to throw the research microwaves such as leaf amount, fixing processing mode
Influence of the different fixing mode of fixing, blanching fixing, three kinds of steam beating to vine tea quality, has obtained optimal selenium-enriched vine tea
Preparation technology.
Claims (10)
1. a kind of preparation method of selenium-enriched vine tea, it is characterised in that comprise the following steps：
(1) by the 50-60 parts by weight of soybean dregs of rice, 10-20 parts by weight Castor seed Meal, 0.8-1.8 parts by weight streptococcus lactis, 0.4-0.9 weights
Fermented after amount part S. cervisiae, 10-20 parts by weight water are well mixed, obtain microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 125-135 parts by weight chicken manure, 155-165 parts by weight powdered rice hulls, 15-25 parts by weight mistakes
Calcium phosphate, 5-15 parts by weight of urea, 15-25 parts sulfuric acids potassium, 1-3 parts sulfuric acids zinc, 3-9 parts by weight microbial adsorbents
It is well mixed, obtain nutrition compound fertilizer；
(3) nutrition compound fertilizer 1000-3000kg is applied in every mu of soil；
(4) vine tea nursery stock is colonized；
(5) the vine tea tender leaf after harvesting is colonized 2 years is finished；
(6) kneaded, be drying to obtain after finishing.
2. the preparation method of selenium-enriched vine tea as claimed in claim 1, it is characterised in that comprise the following steps：
(1) by the 50-60 parts by weight of soybean dregs of rice, 10-20 parts by weight Castor seed Meal, 0.8-1.8 parts by weight streptococcus lactis, 0.4-0.9 weights
Fermented after amount part S. cervisiae, 10-20 parts by weight water are well mixed, obtain microbial fermentation fertilizer；
(2) by microbial fermentation fertilizer and 125-135 parts by weight chicken manure, 155-165 parts by weight powdered rice hulls, 15-25 parts by weight mistakes
Calcium phosphate, 5-15 parts by weight of urea, 15-25 parts sulfuric acids potassium, 1-3 parts sulfuric acids zinc, 3-9 parts by weight microbial adsorbents
It is well mixed, obtain nutrition compound fertilizer；
(3) nutrition compound fertilizer 1000-3000kg is applied in every mu of soil, ploughs deeply 20-30 centimetres of ground, will roll up the soil come break into pieces with
Nutrition compound fertilizer is well mixed；
(4) vine tea nursery stock is colonized, field planting specification is using spacing in the rows × line-spacing as 1.5m × 1.7m, often mu plant 220-320
Strain, annual March, August respectively apply nutrition compound fertilizer once after field planting, apply nutrition compound fertilizer 1000- for every mu every time
2000kg；
(5) the vine tea tender leaf after harvesting is colonized 2 years is finished；
(6) kneaded, be drying to obtain after finishing.
3. the preparation method of selenium-enriched vine tea as claimed in claim 1 or 2, it is characterised in that：The temperature of the fermentation is 25-35
DEG C, pH is 4-6.5, and fermentation time is that stir speed (S.S.) is 20-80 revs/min with stirring in 2-4 days, fermentation process.
4. the preparation method of selenium-enriched vine tea as claimed in claim 1 or 2, it is characterised in that：The microbial adsorbent is leech
One or more mixture in stone flour, humic acid, turf.
5. the preparation method of selenium-enriched vine tea as claimed in claim 4, it is characterised in that：The microbial adsorbent is modified leech
Stone flour.
6. the preparation method of selenium-enriched vine tea as claimed in claim 5, it is characterised in that：The PERFORMANCE OF MODIFIED VERMICULITE powder is by following methods
Prepare：It is 1 in mass ratio by 0.3-0.8mol/L ammonium sulfates and vermiculite power：5 mixing, immersion is centrifuged after 12-36 hours, is dried
Do, as PERFORMANCE OF MODIFIED VERMICULITE powder.
7. the preparation method of selenium-enriched vine tea as claimed in claim 1 or 2, it is characterised in that：It is described fixing for frying fixing, it is micro-
Ripple finishes or steam beating.
8. the preparation method of selenium-enriched vine tea as claimed in claim 7, it is characterised in that：The fixing is microwave de-enzyming.
9. the preparation method of selenium-enriched vine tea as claimed in claim 1 or 2, it is characterised in that：It in temperature is 90- that the drying, which is,
110℃。
10. a kind of selenium-enriched vine tea, it is characterised in that：It is prepared from using method any one of claim 1-9.
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| CN201710414780.8A CN107242072A (en) | 2017-06-05 | 2017-06-05 | Selenium-enriched vine tea and preparation method thereof |
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Family
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| CN201710414780.8A Pending CN107242072A (en) | 2017-06-05 | 2017-06-05 | Selenium-enriched vine tea and preparation method thereof |
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| CN108076942A (en) * | 2017-12-14 | 2018-05-29 | 恩施土家族苗族自治州农业科学院 | A kind of cultural method of selenium-enriched vine tea |
| CN109548548A (en) * | 2018-12-27 | 2019-04-02 | 湖南乾坤生物科技有限公司 | A kind of ampelopsis grossdentata seedling breeding and close short early cultural method |
| CN109566807A (en) * | 2018-11-30 | 2019-04-05 | 龚士华 | Selenium-enriched vine tea and its processing method |
| CN112159290A (en) * | 2020-10-12 | 2021-01-01 | 湖南农业大学 | Strawberry and tea organic fertilizer and preparation method and application thereof |
| CN113367215A (en) * | 2021-06-08 | 2021-09-10 | 江口县铜江生物科技有限公司 | Wild vine tea making method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108076942A (en) * | 2017-12-14 | 2018-05-29 | 恩施土家族苗族自治州农业科学院 | A kind of cultural method of selenium-enriched vine tea |
| CN109566807A (en) * | 2018-11-30 | 2019-04-05 | 龚士华 | Selenium-enriched vine tea and its processing method |
| CN109548548A (en) * | 2018-12-27 | 2019-04-02 | 湖南乾坤生物科技有限公司 | A kind of ampelopsis grossdentata seedling breeding and close short early cultural method |
| CN112159290A (en) * | 2020-10-12 | 2021-01-01 | 湖南农业大学 | Strawberry and tea organic fertilizer and preparation method and application thereof |
| CN113367215A (en) * | 2021-06-08 | 2021-09-10 | 江口县铜江生物科技有限公司 | Wild vine tea making method |
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Application publication date: 20171013 |