CN107213525A - A kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune - Google Patents

A kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune Download PDF

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Publication number
CN107213525A
CN107213525A CN201710404720.8A CN201710404720A CN107213525A CN 107213525 A CN107213525 A CN 107213525A CN 201710404720 A CN201710404720 A CN 201710404720A CN 107213525 A CN107213525 A CN 107213525A
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solution
nostoc commune
preparation
repair materials
regenerated
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王吉鹏
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Hefei Create Technology Co Ltd
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Hefei Create Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
    • D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
    • D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
    • D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/23—Carbohydrates
    • A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Mechanical Engineering (AREA)
  • Textile Engineering (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Artificial Filaments (AREA)
  • Materials For Medical Uses (AREA)

Abstract

A kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune, belongs to tissue renovation material preparing technical field, including：Repair materials are made in the preparation of distilled Nostoc Commune, the preparation of acetic acid/lignin liquor, the preparation of regenerated cellulose spinning solution, electrostatic spinning.Repair materials produced by the present invention have good anti-microbial property, mechanical performance, tissue repair performance and drug attachment.

Description

A kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune
Technical field
The invention belongs to tissue renovation material preparing technical field, more particularly to a kind of composite regenerated fiber reparation of Nostoc commune The preparation method of material.
Background technology
Cellulose as natural biopolymer, with biodegradability, the physics of biocompatibility and uniqueness, Chemistry and mechanical performance, it has also become the study hotspot in new bio medical material field.And prior art is fine using regeneration mostly Dimension element is blended with other materials, and freeze-drying prepares composite membrane, using be combined made from the method film uniformity, stability compared with Difference, and tissue repairing ability and drug attachment need further raising.
The content of the invention
In order to solve problems of the prior art, the invention provides it is a kind of with good tissue repairing ability and The preparation method of the composite regenerated fiber repair materials of Nostoc commune of drug attachment.
The present invention uses following technical scheme：
A kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune, comprises the following steps：
Step one：Nostoc commune is cleaned, and is placed at aeration-drying and is air-dried, 90 DEG C of conditions are placed in after air-drying Lower dry 5min, is put into pulverizer after drying and crushes, and crosses 60 mesh sieves, obtains Nostoc commune powder；
Step 2：2g Nostoc commune powder is weighed, is placed in the wide-mouth bottle for filling 100ml absolute ethyl alcohols, in 40 DEG C of temperature conditionss Lower water bath with thermostatic control, extracts 10h, filter residue is taken out after solution is filtered, and 50ml absolute ethyl alcohol is added into filter residue and in 40 DEG C of bars Constant temperature extracts 60min under part, filtrate will merge twice after filtering again, and carries out rotary evaporation under the conditions of 58 DEG C, concentrates To the 1/5 of original volume, distilled Nostoc Commune is obtained；
Step 3：Acetic acid, tetrahydrofuran in mass ratio 1:1 mixing, dissolves cellulose acetate, being prepared into mass fraction is 10% cellulose acetate solution；
Step 4：Acetic acid lignin, is prepared into the lignin liquor that mass fraction is 20%；
Step 5：Cellulose acetate solution, lignin liquor in mass ratio 7:3 mixing, and shaken on vortex mixer It is well mixed, acetic acid/lignin liquor is made；
Step 6：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1: 50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 7：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made, CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning Liquid；
Step 8：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 9：By regenerated silk fibroin spinning solution and cellulose solution by weight 3:1 is well mixed to obtain regenerated fiber Plain spinning solution；
Step 10：By distilled Nostoc Commune, acetic acid/lignin liquor, regenerated cellulose spinning solution in mass ratio 3:4:25 It is well mixed, it is disperseed using ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir at room temperature 8h, obtains viscous solution and is placed in the medical needle tubing being connected with high-pressure installation, and repair materials are made by electrostatic spinning, and height press-fits Voltage is put for 20kV, spinning flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, at 40 DEG C Lower vacuum drying.
It is preferred that, the capacity of the medical needle tubing described in step 10 is 20ml.
It is preferred that, the syringe needle of the medical needle tubing described in step 10 is fixed at electrostatic spinning receiver board 20cm.
It is preferred that, the syringe needle internal diameter of the medical needle tubing described in step 10 is 0.41mm.
The beneficial effects of the present invention are：
1) Nostoc commune burns rich in mineral matters such as protein, multivitamin and phosphorus, zinc, calcium with clearing heat and detoxicating, treatment Effect of wound, scald and sore swollen toxin.
2) acetic acid and lignin have excellent biodegradability and biocompatibility, and can improve the power of composite Learn performance.
3) fibroin albumen has good antibiotic property, biocompatibility and tissue repairing ability.
4) cellulose has good biocompatibility, degradability and excellent mechanical performance.
5) cellulose can improve the mechanical performance of material, and fibroin albumen can improve materials microstructure repair ability.
6) material prepared using electrostatic spinning technique, is further increased the porosity of material, is conducive to the attached of medicine .
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune, comprises the following steps：
Step one：Nostoc commune is cleaned, and is placed at aeration-drying and is air-dried, 90 DEG C of conditions are placed in after air-drying Lower dry 5min, is put into pulverizer after drying and crushes, and crosses 60 mesh sieves, obtains Nostoc commune powder；
Step 2：2g Nostoc commune powder is weighed, is placed in the wide-mouth bottle for filling 100ml absolute ethyl alcohols, in 40 DEG C of temperature conditionss Lower water bath with thermostatic control, extracts 10h, filter residue is taken out after solution is filtered, and 50ml absolute ethyl alcohol is added into filter residue and in 40 DEG C of bars Constant temperature extracts 60min under part, filtrate will merge twice after filtering again, and carries out rotary evaporation under the conditions of 58 DEG C, concentrates To the 1/5 of original volume, distilled Nostoc Commune is obtained；
Step 3：Acetic acid, tetrahydrofuran in mass ratio 1:1 mixing, dissolves cellulose acetate, being prepared into mass fraction is 10% cellulose acetate solution；
Step 4：Acetic acid lignin, is prepared into the lignin liquor that mass fraction is 20%；
Step 5：Cellulose acetate solution, lignin liquor in mass ratio 7:3 mixing, and shaken on vortex mixer It is well mixed, acetic acid/lignin liquor is made；
Step 6：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1: 50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 7：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made, CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning Liquid；
Step 8：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 9：By regenerated silk fibroin spinning solution and cellulose solution by weight 3:1 is well mixed to obtain regenerated fiber Plain spinning solution；
Step 10：By distilled Nostoc Commune, acetic acid/lignin liquor, regenerated cellulose spinning solution in mass ratio 3:4:25 It is well mixed, it is disperseed using ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir at room temperature 8h, obtains viscous solution and is placed in the medical needle tubing being connected with high-pressure installation, and repair materials are made by electrostatic spinning, and height press-fits Voltage is put for 20kV, spinning flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, at 40 DEG C Lower vacuum drying.
The capacity of medical needle tubing described in step 10 is 20ml, and the syringe needle of described medical needle tubing is fixed on apart from electrostatic At spinning receiver board 20cm, syringe needle internal diameter is 0.41mm.

Claims (4)

1. a kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune, it is characterised in that comprise the following steps：
Step one：Nostoc commune is cleaned, and is placed at aeration-drying and is air-dried, is done under the conditions of being placed in 90 DEG C after air-drying Dry 5min, is put into pulverizer after drying and crushes, and crosses 60 mesh sieves, obtains Nostoc commune powder；
Step 2：2g Nostoc commune powder is weighed, is placed in the wide-mouth bottle for filling 100ml absolute ethyl alcohols, it is permanent under 40 DEG C of temperature conditionss Tepidarium, extracts 10h, filter residue is taken out after solution is filtered, and 50ml absolute ethyl alcohol is added into filter residue and under the conditions of 40 DEG C Constant temperature extracts 60min, filtrate will merge twice after filtering again, and carries out rotary evaporation under the conditions of 58 DEG C, is concentrated into original The 1/5 of volume, obtains distilled Nostoc Commune；
Step 3：Acetic acid, tetrahydrofuran in mass ratio 1:1 mixing, dissolves cellulose acetate, and it is 10% to be prepared into mass fraction Cellulose acetate solution；
Step 4：Acetic acid lignin, is prepared into the lignin liquor that mass fraction is 20%；
Step 5：Cellulose acetate solution, lignin liquor in mass ratio 7:3 mixing, and concussion makes it on vortex mixer It is well mixed, acetic acid/lignin liquor is made；
Step 6：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1:50, remove Silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, be washed with deionized water Only, degumming fibroin is obtained after air-drying；
Step 7：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, the CaCl that concentration is 5wt.% is made2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin solution, room The lower drying and forming-film of temperature, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk fibroin film by film with deionized water Secondary under room temperature state to be dissolved in formic acid, bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning solution；
Step 8：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 9：By regenerated silk fibroin spinning solution and cellulose solution by weight 3:1 is well mixed that regenerated cellulose is spun Silk liquid；
Step 10：By distilled Nostoc Commune, acetic acid/lignin liquor, regenerated cellulose spinning solution in mass ratio 3:4:25 mixing Uniformly, it is disperseed using ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continues to stir 8h at room temperature, obtained It is placed in viscous solution in the medical needle tubing being connected with high-pressure installation, repair materials, high-pressure installation electricity is made by electrostatic spinning Press as 20kV, spinning flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, it is true at 40 DEG C Sky is dried.
2. a kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune according to claim 1, it is characterised in that： The capacity of medical needle tubing described in step 10 is 20ml.
3. a kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune according to claim 1, it is characterised in that： The syringe needle of medical needle tubing described in step 10 is fixed at electrostatic spinning receiver board 20cm.
4. a kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune according to claim 1, it is characterised in that： The syringe needle internal diameter of medical needle tubing described in step 10 is 0.41mm.
CN201710404720.8A 2017-06-01 2017-06-01 A kind of preparation method of the composite regenerated fiber repair materials of Nostoc commune Pending CN107213525A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109395061A (en) * 2018-12-12 2019-03-01 广州汇朗无纺制品有限公司 A kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103341209A (en) * 2013-07-08 2013-10-09 苏州大学 Silk fibroin nanofiber membrane and preparation method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103341209A (en) * 2013-07-08 2013-10-09 苏州大学 Silk fibroin nanofiber membrane and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109395061A (en) * 2018-12-12 2019-03-01 广州汇朗无纺制品有限公司 A kind of composite membrane for the nursing of skin lesions of pemphigus vulgaris position

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