CN107012525A - A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan - Google Patents
A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan Download PDFInfo
- Publication number
- CN107012525A CN107012525A CN201710404524.0A CN201710404524A CN107012525A CN 107012525 A CN107012525 A CN 107012525A CN 201710404524 A CN201710404524 A CN 201710404524A CN 107012525 A CN107012525 A CN 107012525A
- Authority
- CN
- China
- Prior art keywords
- solution
- chitosan
- preparation
- repair materials
- regenerated cellulose
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Classifications
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F2/00—Monocomponent artificial filaments or the like of cellulose or cellulose derivatives; Manufacture thereof
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
- D01F1/103—Agents inhibiting growth of microorganisms
Abstract
A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan, belongs to tissue renovation material preparing technical field, including：Repair materials are made in the preparation of chitosan-acetic acid solution, the preparation of regenerated cellulose spinning solution, electrostatic spinning.Tissue renovation material produced by the present invention has good anti-microbial property, mechanical performance, tissue repair performance and drug attachment.
Description
Technical field
The invention belongs to tissue renovation material preparing technical field, more particularly to a kind of composite regenerated cellulose group of chitosan
Knit the preparation method of repair materials.
Background technology
Cellulose as natural biopolymer, with biodegradability, the physics of biocompatibility and uniqueness,
Chemistry and mechanical performance, it has also become the study hotspot in new bio medical material field.And prior art is fine using regeneration mostly
Dimension element is blended with other materials, and freeze-drying prepares composite membrane, using be combined made from the method film uniformity, stability compared with
Difference, and tissue repairing ability and drug attachment need further raising.
The content of the invention
In order to solve problems of the prior art, the invention provides it is a kind of with good tissue repairing ability and
The preparation method of the composite regenerated cellulose tissue repair materials of chitosan of drug attachment.
The present invention uses following technical scheme：
A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan, comprises the following steps：
Step one：By chitosan and the acetic acid of mass concentration 1% by weight 1:Magnetic agitation 2h under 8 mixing, normal temperature, turns
Fast 1000r/min, is made chitosan-acetic acid solution；
Step 2：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1:
50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization
Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 3：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made,
CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten
Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature
Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning
Liquid；
Step 4：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 5：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed to obtain regenerated fiber
Plain spinning solution；
Step 6：By chitosan-acetic acid solution and regenerated cellulose spinning solution in mass ratio 3:20 are well mixed, using super
Sound wave shock disperses to it, magnetic agitation 1h under the conditions of 50 DEG C, then continues to stir 8h at room temperature, obtains viscous solution and puts
In the medical needle tubing being connected with high-pressure installation, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, spinning
Flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, is dried in vacuo at 40 DEG C.
It is preferred that, the capacity of the medical needle tubing described in step 6 is 20ml.
It is preferred that, the syringe needle of the medical needle tubing described in step 6 is fixed at electrostatic spinning receiver board 20cm.
It is preferred that, the syringe needle internal diameter of the medical needle tubing described in step 6 is 0.41mm.
The beneficial effects of the present invention are：
1) fibroin albumen and chitosan have good antibiotic property, biocompatibility and certain tissue repairing ability.
2) cellulose has good biocompatibility, degradability and excellent mechanical performance.
3) cellulose can improve the mechanical performance of material, and fibroin albumen and chitosan can improve materials microstructure reparation
Ability.
4) material prepared using electrostatic spinning technique, is further increased the porosity of material, is conducive to the attached of medicine
.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan, comprises the following steps：
Step one：By chitosan and the acetic acid of mass concentration 1% by weight 1:Magnetic agitation 2h under 8 mixing, normal temperature, turns
Fast 1000r/min, is made chitosan-acetic acid solution；
Step 2：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1:
50, silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, use deionization
Water is cleaned, and degumming fibroin is obtained after air-drying；
Step 3：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, it is 5wt.%'s that concentration, which is made,
CaCl2- FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin molten
Liquid, drying and forming-film, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk egg by film with deionized water at room temperature
Tunica albuginea is secondary under room temperature state to be dissolved in formic acid, and bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning
Liquid；
Step 4：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 5：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed to obtain regenerated fiber
Plain spinning solution；
Step 6：By chitosan-acetic acid solution and regenerated cellulose spinning solution in mass ratio 3:20 are well mixed, using super
Sound wave shock disperses to it, magnetic agitation 1h under the conditions of 50 DEG C, then continues to stir 8h at room temperature, obtains viscous solution and puts
In the medical needle tubing being connected with high-pressure installation, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, spinning
Flow 2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, is dried in vacuo at 40 DEG C.
The capacity of medical needle tubing described in step 6 is 20ml, and the syringe needle of described medical needle tubing is fixed on apart from electrostatic
At spinning receiver board 20cm, syringe needle internal diameter is 0.41mm.
Claims (4)
1. a kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan, it is characterised in that comprise the following steps：
Step one：By chitosan and the acetic acid of mass concentration 1% by weight 1:Magnetic agitation 2h, rotating speed under 8 mixing, normal temperature
1000r/min, is made chitosan-acetic acid solution；
Step 2：Silk cocoon is removed into pupa, the mass concentration boiled is inserted in 0.5% aqueous sodium carbonate, bath raio is 1:50, remove
Silk cocoon surface silk gum is removed, will pull out and wring out into thread silk cocoon, after repeating aforesaid operations once, be washed with deionized water
Only, degumming fibroin is obtained after air-drying；
Step 3：By CaCl2It is dissolved under room temperature state in formic acid, stirs 30min, the CaCl that concentration is 5wt.% is made2-
FA solution, is then dissolved in CaCl by degumming fibroin2In-FA solution, magnetic agitation 3h obtains regenerated silk fibroin solution, room
The lower drying and forming-film of temperature, soaks 3h, re-dry obtains regenerated silk fibroin film, by regenerated silk fibroin film by film with deionized water
Secondary under room temperature state to be dissolved in formic acid, bath raio is 1:10, dissolution time is 2h, obtains regenerated silk fibroin spinning solution；
Step 4：Cotton linter 5g is taken, is dissolved in formic acid, the cellulose solution of mass concentration 4% is configured to；
Step 5：By regenerated silk fibroin spinning solution and cellulose solution by weight 4:1 is well mixed that regenerated cellulose is spun
Silk liquid；
Step 6：By chitosan-acetic acid solution and regenerated cellulose spinning solution in mass ratio 3:20 are well mixed, and use ultrasonic wave
Concussion it is disperseed, magnetic agitation 1h under the conditions of 50 DEG C, then at room temperature continue stir 8h, obtain viscous solution be placed in
In the medical needle tubing of high-pressure installation connection, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, spinning flow
2ml/h, obtained material is placed in the ethanol of volumetric concentration 75% and soaks 30min, is dried in vacuo at 40 DEG C.
2. a kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan according to claim 1, it is special
Levy and be：The capacity of medical needle tubing described in step 6 is 20ml.
3. a kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan according to claim 1, it is special
Levy and be：The syringe needle of medical needle tubing described in step 6 is fixed at electrostatic spinning receiver board 20cm.
4. a kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan according to claim 1, it is special
Levy and be：The syringe needle internal diameter of medical needle tubing described in step 6 is 0.41mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710404524.0A CN107012525A (en) | 2017-06-01 | 2017-06-01 | A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan |
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|---|---|---|---|
| CN201710404524.0A CN107012525A (en) | 2017-06-01 | 2017-06-01 | A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan |
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| CN201710404524.0A Pending CN107012525A (en) | 2017-06-01 | 2017-06-01 | A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112280058A (en) * | 2020-07-13 | 2021-01-29 | 广西科技大学 | Preparation method of fibrillated silk chitosan composite material |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1037018A (en) * | 1996-07-16 | 1998-02-10 | Fuji Spinning Co Ltd | Antibacterial cellulose regenerated fiber |
| CN101445971A (en) * | 2008-12-19 | 2009-06-03 | 东华大学 | Method for preparing bionic extracellular matrix silk fibroin/chitosan composite nanometer fibre |
| CN103436985A (en) * | 2013-07-22 | 2013-12-11 | 苏州三和开泰花线织造有限公司 | Preparation method of silk fibroin/chitosan blend nanofiber |
| CN103498210A (en) * | 2013-08-21 | 2014-01-08 | 浙江理工大学 | Biomimetic preparation method of high-strength regenerated silk protein fiber |
| CN103993422A (en) * | 2014-04-23 | 2014-08-20 | 东华大学 | Regenerative silk fibroin/chitosan derivative blend fibrofelt and preparing method thereof |
| CN104892969A (en) * | 2015-06-23 | 2015-09-09 | 武汉工程大学 | Composite chitosan and cellulose membrane and preparation method and application thereof |
| CN105544210A (en) * | 2016-01-21 | 2016-05-04 | 苏州印丝特纺织数码科技有限公司 | Fabric post-finishing agent based on modified silk fibroin solution and preparation method of fabric post-finishing agent |
| CN106479195A (en) * | 2016-10-26 | 2017-03-08 | 武汉纺织大学 | A kind of nano-cellulose strengthens fibroin albumen composite material and preparation method thereof |
-
2017
- 2017-06-01 CN CN201710404524.0A patent/CN107012525A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1037018A (en) * | 1996-07-16 | 1998-02-10 | Fuji Spinning Co Ltd | Antibacterial cellulose regenerated fiber |
| CN101445971A (en) * | 2008-12-19 | 2009-06-03 | 东华大学 | Method for preparing bionic extracellular matrix silk fibroin/chitosan composite nanometer fibre |
| CN103436985A (en) * | 2013-07-22 | 2013-12-11 | 苏州三和开泰花线织造有限公司 | Preparation method of silk fibroin/chitosan blend nanofiber |
| CN103498210A (en) * | 2013-08-21 | 2014-01-08 | 浙江理工大学 | Biomimetic preparation method of high-strength regenerated silk protein fiber |
| CN103993422A (en) * | 2014-04-23 | 2014-08-20 | 东华大学 | Regenerative silk fibroin/chitosan derivative blend fibrofelt and preparing method thereof |
| CN104892969A (en) * | 2015-06-23 | 2015-09-09 | 武汉工程大学 | Composite chitosan and cellulose membrane and preparation method and application thereof |
| CN105544210A (en) * | 2016-01-21 | 2016-05-04 | 苏州印丝特纺织数码科技有限公司 | Fabric post-finishing agent based on modified silk fibroin solution and preparation method of fabric post-finishing agent |
| CN106479195A (en) * | 2016-10-26 | 2017-03-08 | 武汉纺织大学 | A kind of nano-cellulose strengthens fibroin albumen composite material and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112280058A (en) * | 2020-07-13 | 2021-01-29 | 广西科技大学 | Preparation method of fibrillated silk chitosan composite material |
| CN112280058B (en) * | 2020-07-13 | 2023-09-19 | 广西科技大学 | Preparation method of fibrillated silk fibroin chitosan composite material |
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