CN107201301A - A kind of multistage rock type bioreactor - Google Patents
A kind of multistage rock type bioreactor Download PDFInfo
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- CN107201301A CN107201301A CN201710330464.2A CN201710330464A CN107201301A CN 107201301 A CN107201301 A CN 107201301A CN 201710330464 A CN201710330464 A CN 201710330464A CN 107201301 A CN107201301 A CN 107201301A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
A kind of multistage rock type bioreactor, including the annular perturbator of stepwise, the drive device for providing driving force and the controller for drive device for supporting the multistage rock type platform of disposable biological reaction bag, for increasing fixed plate supporting zone bottom nutrient solution less turbulence, multistage rock type platform and the annular perturbator of stepwise are affixed, drive device is arranged at multistage shake platform, and drive device control end is electrically connected with controller respective pins；Multistage rock type platform includes polygonal flat panel and swing plate；Stepwise annular perturbator includes multiple sleeves being coaxially socketed and multi-stage telescopic cylinder, and adjacent sleeve, which is slidably connected, to be arranged into ring-like, and length is decrescence from inside to outside for sleeve；Drive device includes the auxiliary reclay of drive cylinder, reversal valve and control reversal valve work.The beneficial effects of the invention are as follows：Nutrient solution bottom less turbulence is big, gas transfer efficiency high, and that improves platform production capacity can scale-up version.
Description
Technical field
The present invention relates to the multistage rock type bioreactor of one kind.
Background technology
Biotechnology has shown huge economic value and social value at present as a kind of emerging technology, hair
Open up current mainly including technique for gene engineering, Fermentation Engineering, cell engineering, enzyme engineering and protein engineering etc..These works
Journey technology is all involved in cell metabolism and cell culture from technical research to last large-scale production.It is directed to thin
The research of the key equipment bioreactor of born of the same parents' culture turns into one of core technology important in biotechnology.
With the development of bioreactor technology, the development of especially disposable technology, disposable biological respinse
Device is widely used to seed and expands culture, the training of small mammalian cell, plant cell and insect cell to pilot-scale
Support, the production of monoclonal antibody, vaccine and recombinant protein etc., and be gradually extended to the fields such as microbial fermentation, algae culture.Its
Maximum commercial use is to be used to seed expand culture and culture mammalian cell production antibody, vaccine etc..
In existing disposable bioreactor species, the hybrid-type disposable bioreactor of wave is carried earliest
Go out.The hybrid-type disposable bioreactor critical piece of wave is the disposable biological reaction bag of a sterilizing, its cell bags
Be placed on shake platform, the shake of platform makes culture medium produce wave, these waves to culture provide sufficiently mixing with
Oxygen is transmitted.This single support, the bioreactor of Integral rocking formula, are owed in terms of shape wave state is made and in terms of controllability
Lack, it is difficult to which high-quality gas transfer process is provided.
It analyzes a kind of " sit-ups formula " and shakes platform in a kind of shake platform patent for bioreactor
Deficiency, and propose a kind of rock type platform of monopole, be effectively improved bioreactor make unrestrained ability and platform can
Regulation performance.
But the above-mentioned shake platform being previously mentioned is when in face of higher volume of production application, it is difficult to provide higher-quality make
Unrestrained ability, the nutrient solution bottom less turbulence of fixed plate supporting zone is small, and gas transfer is inefficient, and this have impact on platform more
The applicability of wide application, limit platform production capacity can amplification.
The content of the invention
When the present invention is directed to current rock type flat surface to higher volume of production application, it is difficult to provide higher-quality
Unrestrained ability is made, the nutrient solution bottom less turbulence of fixed plate supporting zone is small, the problem of gas transfer is inefficient, proposed a kind of
Nutrient solution bottom less turbulence is big, the multistage rock type bioreactor of gas transfer efficiency high.
The multistage rock type bioreactor of one kind of the present invention, it is characterised in that：Including for supporting disposable life
The multistage rock type platform of thing reaction bag, the stepwise annular for increasing fixed plate supporting zone bottom nutrient solution less turbulence
Perturbator, the drive device for providing driving force and the controller for drive device, the multistage rock type platform with
The annular perturbator of the stepwise is affixed, and the drive device is arranged at the multistage shake platform, and the driving dress
The control end put and the respective pins of the controller are electrically connected；
The multistage rock type platform includes polygonal flat panel and swing plate, and the side number of the polygonal flat panel is even
Number, the polygonal flat panel center is provided with the through hole passed for the annular perturbator of stepwise；The each edge of the polygonal flat panel
Correspondence is hinged one piece of swing plate, wherein two pieces of swing plates are one group, at least two groups shake platforms are moved simultaneously；
The annular perturbator of the stepwise includes multiple sleeves being coaxially socketed and multi-stage telescopic cylinder, and adjacent sleeve is slided
Dynamic connection is arranged into ring-like, and length is decrescence from inside to outside for sleeve；In addition to the sleeve positioned at center, remaining sleeve bottom end face is filled
There is barrier plate, barrier plate is provided with the central through hole passed through for the expansion link of multi-stage telescopic cylinder, and sleeve is corresponded to from inside to outside
Barrier plate central through hole aperture it is cumulative；The flexible series of the expansion link of the multi-stage telescopic cylinder and the number one of sleeve
Cause, and outermost layer sleeve is corresponding with the first order body of rod of the multi-stage telescopic Telescopic-cylinder bar, secondary outer layer sleeve with it is described
The second level body of rod correspondence of multi-stage telescopic cylinder, by that analogy, sleeve and the multi-stage telescopic cylinder positioned at center
Highest body of rod correspondence, the highest body of rod outer end of the expansion link of the multi-stage telescopic cylinder and the sleeve bottom positioned at center
Affixed, the central through hole aperture of outermost layer sleeve is between first order external diameter of rod body and second level external diameter of rod body, secondary outer layer jacket
The central through hole aperture of cylinder is between second level external diameter of rod body and third level external diameter of rod body, by that analogy；The sleeve is with more
The step that the flexible formation of level telescopic cylinder can be passed from polygonal flat panel through hole；The control end of the multi-stage telescopic cylinder
Controlling switch corresponding with the controller is electrically connected；
The drive device include it is some be used for drive swing plate drive cylinder, it is some be used for control drive cylinder to transport
The reversal valve in dynamic direction and the auxiliary reclay of control reversal valve work, and drive cylinder one piece of swing plate of correspondence,
The bottom of drive cylinder is hinged with polygonal flat panel, and the expansion link of drive cylinder is hinged with corresponding swing plate bottom so that
The swing plate is swung up and down using polygonal flat panel surface as boundary；The drive cylinder is corresponded with the reversal valve, and
The air inlet of drive cylinder and gas outlet communication corresponding with reversal valve respectively, and the reversal valve control end in
Between the corresponding controling end of relay and the controller be electrically connected, realize the commutation of drive cylinder.
The reversal valve is 3 position-5 way valve, and each reversal valve configures an A coil for being used to control reversal valve
With the auxiliary reclay of B coil alternate energisations.
One end of the swing plate is consistent with the side of polygonal flat panel, and the other end is semicircular plate, and swing plate to
The upper extreme position of upper swing and the angle of polygonal flat panel are 60 °, lower limit position from swing plate to lower swing with it is polygon
The angle of shape flat board is 30 °.
The annular perturbator of the stepwise is quadravalence annular construction member, i.e., the described annular perturbator of stepwise includes 4 sleeves,
The multi-stage telescopic cylinder is level Four telescopic cylinder, wherein the sleeve bottom positioned at center is provided with for telescopic with level Four
The affixed installation portion of the fourth stage body of rod of cylinder.
The sleeve is cannulated sleeve, and sleeve is up and down sealed.
Same group of two pieces of swing plates are in relative, adjacent or interval position, and same group of swing plate is same
When upwardly or downwardly swing.
Swing plate realizes the swing of certain angle under the drive of cylinder, and the angle and frequency of swing can be carried out adaptively
Regulation.Stepwise annular perturbator is connected with the stepwise circular bottom part of disposable biological reaction bag.Stepwise annular perturbator
The motion of each rank drives the stepwise circular bottom part motion of disposable biological reaction bag, the difference of each rank of stepwise annular perturbator
Motor pattern can cause disposable biological reaction bag fixed plate supporting zone to produce different turbulating effects.Stepwise annular disturbance
Device is typically designed as quadravalence, and the height of its every single order is adjustable within the specific limits, per single order all by a cylinder supports band
It is dynamic, realize the motion of each rank of annular perturbator by the lifting of cylinder.The swing of swing plate and the annular perturbator of stepwise are each
Motion of rank etc. is required for cylinder to complete.Swing plate swing angle and frequency changes need adjust cylinder lift with
The speed of intake and exhaust.Controller can adaptively control movable number of cylinders, be adaptively adjusted the liter of cylinder according to demand
Journey and intake and exhaust speed.
The beneficial effects of the invention are as follows：Swing plate realizes the swing of certain angle, the angle of swing under the drive of cylinder
Degree and frequency can carry out automatic adjusument；A variety of shake states are provided, and by stepwise annular perturbator bottom are trained
Nutrient solution less turbulence is big, so that when in face of higher volume of production application, unrestrained ability, and center are made with higher-quality
Fixed plate supporting zone nutrient solution bottom less turbulence it is big, gas transfer efficiency high improves the wider application of platform
Applicability, improve platform production capacity can amplification.
Brief description of the drawings
Fig. 1 is the rock type platform of two-stage of the present invention；
Fig. 2 is the rock type platform of three-level of the present invention；
Fig. 3 is that two-stage shakes platform front view；
Fig. 4 is annular perturbator pie graph；
Fig. 5 is annular perturbator action diagram；
Fig. 6 is the control drawing of single drive cylinder；
Fig. 7 is the rock type biological case study on implementation figure of two-stage
Fig. 8 is multicomponent dynamic gas mixer schematic diagram.
Embodiment
The present invention is further illustrated below in conjunction with the accompanying drawings
Referring to the drawings：
The multistage rock type bioreactor of the one kind of the present invention of embodiment 1, including for supporting disposable biological anti-
Answer the multistage rock type platform 1 of bag, disturbed for increasing the stepwise annular of fixed plate supporting zone bottom nutrient solution less turbulence
Dynamic device 2, the drive device for providing driving force and the controller for drive device, the multistage rock type platform 1 with
The annular perturbator 2 of the stepwise is affixed, and the drive device is arranged at the multistage shake platform 1, and the driving
The control end of device and the respective pins of the controller are electrically connected；
The multistage rock type platform 1 includes polygonal flat panel 11 and swing plate 12, the side of the polygonal flat panel 11
Number is even number, and the center of polygonal flat panel 11 is provided with the through hole passed for the annular perturbator of stepwise；The polygonal flat panel
11 each edge correspondence is hinged one piece of swing plate 12, wherein two pieces of swing plates 12 are one group, at least two groups swing plates
12 move simultaneously；
The annular perturbator 2 of the stepwise includes 4 sleeves 21 and multi-stage telescopic cylinder 22 being coaxially socketed, adjacent set
Cylinder 21, which is slidably connected, to be arranged into ring-like, and length equal difference is decrescence from inside to outside for sleeve 21；In addition to the sleeve positioned at center, remaining sleeve
Bottom end face is respectively arranged with barrier plate 23, and barrier plate 23 is provided with the center passed through for the expansion link of multi-stage telescopic cylinder 22 and led to
Hole, corresponding barrier plate central through hole aperture equal difference is cumulative from inside to outside for sleeve；The expansion link of the level Four telescopic cylinder
Flexible series is consistent with the number of sleeve, and the first order body of rod of outermost layer sleeve and the multi-stage telescopic Telescopic-cylinder bar
Correspondence, secondary outer layer sleeve is corresponding with the second level body of rod of the multi-stage telescopic cylinder, by that analogy, positioned at center sleeve and
The fourth stage body of rod correspondence of the level Four telescopic cylinder, the fourth stage body of rod outer end of the expansion link of the level Four telescopic cylinder
Affixed with sleeve bottom positioned at center, the central through hole aperture of outermost layer sleeve is between first order external diameter of rod body and second level bar
Between external footpath, the central through hole aperture of secondary outer layer sleeve between second level external diameter of rod body and third level external diameter of rod body, with
This analogizes；The step that the sleeve can be passed with the flexible formation of level Four telescopic cylinder from polygonal flat panel through hole；Described four
The control end controlling switch corresponding with the controller of level telescopic cylinder is electrically connected；Wherein, level Four telescopic cylinder is defined straight
The footpath most thick body of rod is the first order body of rod, and the first order body of rod 221, the second level body of rod 222, the third level body of rod are followed successively by from top to bottom
223 and the fourth stage body of rod 224；Sleeve is defined as the first rank 211, second-order 212, the 3rd rank 213 and successively from inside to outside
Quadravalence 214；
The drive device include it is some be used for drive swing plate drive cylinder 3, it is some be used for control drive cylinder
The reversal valve 4 of the direction of motion and the auxiliary reclay of control reversal valve work, and one piece of a drive cylinder correspondence shakes flat
Plate, bottom and the polygonal flat panel of drive cylinder are hinged, and the expansion link of drive cylinder 3 is cut with scissors with the corresponding bottom of swing plate 12
Connect so that the swing plate 12 is swung up and down using polygonal flat panel surface as boundary；The drive cylinder and the reversal valve 4 one
One correspondence, and the air inlet 31 and the communication corresponding with reversal valve, and the reversal valve respectively of gas outlet 32 of drive cylinder 3
4 control end is electrically connected by the corresponding controling end of auxiliary reclay and the controller, realizes the commutation of drive cylinder.
The reversal valve 4 is 3 position-5 way valve, and each reversal valve configures an A coil for being used to control reversal valve
41 with the auxiliary reclay of the alternate energisation of B coils 42.
One end of the swing plate is consistent with the side of polygonal flat panel, and the other end is semicircular plate, and swing plate to
The upper extreme position of upper swing and the angle of polygonal flat panel are 60 °, lower limit position from swing plate to lower swing with it is polygon
The angle of shape flat board is 30 °.
The annular perturbator of the stepwise is quadravalence annular construction member, i.e., the described annular perturbator of stepwise includes 4 sleeves,
The multi-stage telescopic cylinder is level Four telescopic cylinder, wherein the sleeve bottom positioned at center is provided with for telescopic with level Four
The affixed installation portion of the fourth stage body of rod of cylinder.
The sleeve is cannulated sleeve, and sleeve is up and down sealed.
Same group of two pieces of swing plates are in relative, adjacent or interval position, and same group of swing plate is same
When upwardly or downwardly swing.
Swing plate realizes the swing of certain angle under the drive of cylinder, and the angle and frequency of swing can be carried out adaptively
Regulation.Stepwise annular perturbator is connected with the stepwise circular bottom part of disposable biological reaction bag.Stepwise annular perturbator
The motion of each rank drives the stepwise circular bottom part motion of disposable biological reaction bag, the difference of each rank of stepwise annular perturbator
Motor pattern can cause disposable biological reaction bag fixed plate supporting zone to produce different turbulating effects.Stepwise annular disturbance
Device is typically designed as quadravalence, and the height of its every single order is adjustable within the specific limits, per single order all by a cylinder supports band
It is dynamic, realize the motion of each rank of annular perturbator by the lifting of cylinder.The swing of swing plate and the annular perturbator of stepwise are each
Motion of rank etc. is required for cylinder to complete.Swing plate swing angle and frequency changes need adjust cylinder lift with
The speed of intake and exhaust.Controller can adaptively control movable number of cylinders, be adaptively adjusted the liter of cylinder according to demand
Journey and intake and exhaust speed.
The rock type platform of two-stage for supporting disposable biological reaction bag, as shown in Figure 2.Stepwise annular is disturbed in figure three
Dynamic device is removed.The rock type platform of two-stage is made up of a fixed quadrangle flat board and the two poles of the earth swing plate, relative two
Swing plate 12 (A and A ', B and B ') and each one group of composition.As shown in figure 3, swing plate is realized under the drive of cylinder
The swing of certain angle, the upper extreme position of swing plate is with the horizontal 60 °, the lower limit position and horizontal plane of swing plate
Into 30 °, swing plate can be swung in the range of this.Each swing plate is driven by a single cylinder, changes gas
The higher limit of the air inflow of cylinder can change the lift of cylinder, and then the pendulum of swing plate can be adjusted in the range of extreme angles
Dynamic angle；The hunting frequency of swing plate can be changed by changing the frequency of cylinder intake and exhaust.
The annular perturbator of stepwise for increasing fixed plate supporting zone bottom nutrient solution less turbulence, as shown in Figure 4.
Stepwise annular perturbator is quadravalence, and the structure of each rank is as shown in figure 5, material selects lighter polytetrafluoroethylene (PTFE).Annular disturbance
It is the cylinder of a level Four below device, under the promotion of cylinder, annular step can be into a kind of state shown in Fig. 5, two-stage swing plate
The wave caused is when by annular stage structure, and its less turbulence will increase, and gas transfer efficiency will be improved.The intake and exhaust of cylinder
Speed is adjustable, and then its fixed plate supporting zone bottom nutrient solution turbulence caused is by variation, quality.
The control loop of single cylinder is as shown in Figure 6.Drive cylinder selects double-acting cylinder, and reversal valve changes for 3 position-5 way
To valve, band two loop As, B, A energizations, valve element moves on to position I, and cylinder liter, A power-off, B is powered, cylinder drop.Pass through middle relay
Device control coil A, B break-make, but the stretching motion of cylinder is realized, and auxiliary reclay is connected with controller, passes through sequencing
Design can realize the Self Adaptive Control of multi cylinder.
Fig. 7 is one embodiment of the rock type bioreactor of two-stage, and controller is PLC, PLC except
, can also be multigroup by the temperature in heating blanket control disposable biological reaction bag, control outside the automatic adjusument of cylinder
Divide the supply of dynamic gas mixer, control charging, inoculation, addition defoamer, acid solution, alkali lye are gathered in biological respinse bag
The work such as communication, the data transfer of the parameters such as pH, DO concentration, ORP, temperature, completion and host computer.
By taking Fig. 8 as an example, gaseous mixture is air, oxygen, CO2And N2, wherein every kind of gas all corresponds to the steel cylinder of storage, respectively
For air steel cylinder, oxygen bottle, CO2Steel cylinder and N2Steel cylinder, and each steel cylinder outlet pipe with corresponding spinner flowmeter
Air inlet, the gas outlet of spinner flowmeter is furnished with corresponding magnetic valve and MFC (mass flow controller), wherein air steel
Bottle is imported in the first blender with oxygen bottle, CO2Steel cylinder and N2Steel cylinder is imported in the second blender, final first blender,
Second blender is imported in the 3rd blender, and the gas outlet of the 3rd blender is used as total gas outlet；Wherein air steel cylinder goes out
On air pipe be furnished with magnetic valve, referring herein to magnetic valve be controlled by PLC, MFC is also controlled by PLC, i.e.,
The multicomponent dynamic air-distributing into disposable biological reaction bag can be achieved.
Content described in this specification embodiment is only enumerating to the way of realization of inventive concept, protection of the invention
Scope is not construed as being only limitted to the concrete form that embodiment is stated, protection scope of the present invention also includes art technology
Personnel according to present inventive concept it is conceivable that equivalent technologies mean.
Claims (6)
1. a kind of multistage rock type bioreactor, it is characterised in that：Including the multistage for supporting disposable biological reaction bag
Rock type platform, the annular perturbator of stepwise for increasing fixed plate supporting zone bottom nutrient solution less turbulence, for carrying
Drive device for driving force and the controller for drive device, the multistage rock type platform and stepwise annular
Perturbator is affixed, and the drive device is arranged at the multistage shake platform, and control end and the institute of the drive device
The respective pins for stating controller are electrically connected；
The multistage rock type platform includes polygonal flat panel and swing plate, and the side number of the polygonal flat panel is even number, institute
State polygonal flat panel center and be provided with the through hole passed for the annular perturbator of stepwise；The each edge correspondence of the polygonal flat panel is cut with scissors
One piece of swing plate is connect, wherein two pieces of swing plates are one group, at least two groups shake platforms are moved simultaneously；
The annular perturbator of the stepwise includes multiple sleeves being coaxially socketed and multi-stage telescopic cylinder, and adjacent sleeve, which is slided, to be connected
Cloth run in into ring-like, length is decrescence from inside to outside for sleeve；In addition to the sleeve positioned at center, remaining sleeve bottom end face is respectively arranged with resistance
Baffle plate, barrier plate is provided with the central through hole passed through for the expansion link of multi-stage telescopic cylinder, sleeve corresponding resistance from inside to outside
Baffle center through-hole aperture is cumulative；The flexible series of the expansion link of the multi-stage telescopic cylinder is consistent with the number of sleeve, and
And outermost layer sleeve is corresponding with the first order body of rod of the multi-stage telescopic Telescopic-cylinder bar, secondary outer layer sleeve is stretched with the multistage
The second level body of rod correspondence of contracting formula cylinder, by that analogy, positioned at the superlative degree of the sleeve and the multi-stage telescopic cylinder at center
Body of rod correspondence, the highest body of rod outer end of the expansion link of the multi-stage telescopic cylinder and the sleeve bottom for being located at center are affixed,
The central through hole aperture of outermost layer sleeve is between first order external diameter of rod body and second level external diameter of rod body, in secondary outer layer sleeve
Heart through-hole aperture is between second level external diameter of rod body and third level external diameter of rod body, by that analogy；The sleeve is with multi-stage expansion
The step that the flexible formation of formula cylinder can be passed from polygonal flat panel through hole；The control end of the multi-stage telescopic cylinder with it is described
The corresponding controlling switch of controller is electrically connected；
The drive device include it is some be used for drive swing plate drive cylinder, it is some be used for control drive cylinder motion side
To reversal valve and control reversal valve work auxiliary reclay, and drive cylinder one piece of swing plate of correspondence, driving
The bottom of cylinder is hinged with polygonal flat panel, and the expansion link of drive cylinder is hinged with corresponding swing plate bottom so that described
Swing plate is swung up and down using polygonal flat panel surface as boundary；The drive cylinder is corresponded with the reversal valve, and driving
The air inlet of cylinder and gas outlet communication corresponding with reversal valve respectively, and the reversal valve control end by centre after
The corresponding controling end of electrical equipment and the controller is electrically connected, and realizes the commutation of drive cylinder.
2. a kind of multistage rock type bioreactor as claimed in claim 1, it is characterised in that：The reversal valve is three five
Logical reversal valve, and each reversal valve configures the middle relay of an A coil and B coil alternate energisations for controlling reversal valve
Device.
3. a kind of multistage rock type bioreactor as claimed in claim 1, it is characterised in that：One end of the swing plate
Consistent with the side of polygonal flat panel, the other end is semicircular plate, and the upper extreme position and polygon that swing plate is swung up
The angle of flat board is 60 °, swing plate to the lower limit position of lower swing and the angle of polygonal flat panel be 30 °.
4. a kind of multistage rock type bioreactor as claimed in claim 1, it is characterised in that：The stepwise annular disturbance
Device is quadravalence annular construction member, i.e., the described annular perturbator of stepwise includes 4 sleeves, and the multi-stage telescopic cylinder is stretched for level Four
Contracting formula cylinder, wherein the sleeve bottom positioned at center, which is provided with, is used for the installation affixed with the fourth stage body of rod of level Four telescopic cylinder
Portion.
5. a kind of multistage rock type bioreactor as claimed in claim 4, it is characterised in that：The sleeve is hollow sleeve
Cylinder, and sleeve up and down seals.
6. a kind of multistage rock type bioreactor as claimed in claim 1, it is characterised in that：Two pieces of same group shake flat
Plate is in relative, adjacent or interval position, and same group of swing plate is upwardly or downwardly swung simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710330464.2A CN107201301B (en) | 2017-05-11 | 2017-05-11 | Multistage shaking type bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710330464.2A CN107201301B (en) | 2017-05-11 | 2017-05-11 | Multistage shaking type bioreactor |
Publications (2)
| Publication Number | Publication Date |
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| CN107201301A true CN107201301A (en) | 2017-09-26 |
| CN107201301B CN107201301B (en) | 2023-10-20 |
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| CN201710330464.2A Active CN107201301B (en) | 2017-05-11 | 2017-05-11 | Multistage shaking type bioreactor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111632636A (en) * | 2020-05-14 | 2020-09-08 | 张彬 | Constant-temperature multi-oscillation water bath shaking table |
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| CN107201301B (en) | 2023-10-20 |
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