CN107199062A - A kind of ultra micro quantity of fluid sample injector - Google Patents
A kind of ultra micro quantity of fluid sample injector Download PDFInfo
- Publication number
- CN107199062A CN107199062A CN201710448968.4A CN201710448968A CN107199062A CN 107199062 A CN107199062 A CN 107199062A CN 201710448968 A CN201710448968 A CN 201710448968A CN 107199062 A CN107199062 A CN 107199062A
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- China
- Prior art keywords
- capillary
- sample
- transfer device
- heat transfer
- ultra micro
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to biochemical test technical field, specially a kind of ultra micro quantity of fluid sample injector.The ultra micro quantity of fluid sample injector of the present invention, including：One internal diameter is that in 1 10 microns, external diameter is the capillary in 2 20 microns, and capillary side wall is provided with inlet；One miniature heat transfer device for being positioned in capillary；One heating source for being connected with miniature heat transfer device, for heating miniature heat transfer device；One micro liquid pump, for ultra micro quantity of fluid to be pumped into capillary from inlet.The capillary can have multiple, forming array sample injector.The present invention carries out point liquid of ultra micro quantity of fluid using the expansion principle of miniature heat transfer device, and the liquid point sample of picoliters rank is realized with this.The present invention solves the sample-adding difficult point that existing micro sample adding appliance is directed to ultra micro quantity of fluid, it is possible to achieve the sample-adding of picoliters rank, improves the utilization rate of micro valuable sample.
Description
Technical field
The invention belongs to biochemical test technical field, and in particular to a kind of ultra micro quantity of fluid sample injector.
Background technology
At present, traditional micro sample adding appliance（Pipettor）Imbibition scope it is general between 0.1-1000 microlitres, especially fit
Used for biological experiment or chemical laboratory.Micro sample adding appliance is developed into today, and kind is also varied, such as micro distribution
Device, multichannel micro sample adding appliance etc., its physics principle being loaded are mainly following two：1. mattress is used（Also known as piston
Stroke）Sample-adding；2. the just mobile sample-adding of piston of no mattress is used.
In addition, occurring a kind of denier sample injector in recent years（Pipettor）, its liquid relief mode is to utilize sound wave pipetting technique
Pipettor is set to enter the liquid relief level of nanoliter level, but minimum liquid relief amount only can reach 2.5 nanoliters.
If, it is necessary to be allocated liquid relief to more micro liquid, existing conventional micro sample adding appliance can not then expire
Foot.
The content of the invention
In order to make up above-mentioned the deficiencies in the prior art, the present invention provides a kind of new ultra micro quantity of fluid sample injector, to reality
The liquid sample-adding of existing picoliters rank.
The ultra micro quantity of fluid sample injector that the present invention is provided, its structure composition includes：
One internal diameter is that in 1-10 microns, external diameter is the capillary in 2-20 microns；Capillary is made up of heat-insulating material；Capillary
Side wall upper part is provided with inlet；
One miniature heat transfer device for being positioned in capillary, such as copper wire or other thermal expansion materials；
One heating source for being connected with miniature heat transfer device, for heating miniature heat transfer device；
One micro liquid pump, for ultra micro quantity of fluid to be pumped into capillary from inlet.
If it is necessary, the capillary can have multiple, forming array sample injector.
In use, micro liquid is pumped into capillary by micro liquid pump；Regulation heating source temperature, passes through heating unit
（Heat conductive filament）Heat is reached into miniature heat transfer device；Miniature heat transfer device expanded by heating, micro liquid is added dropwise from capillary drip
Enter required container.
Ultra micro quantity of fluid sample injector of the present invention utilizes miniature heat transfer device（Such as copper wire）Expansion principle carry out ultramicron
Point liquid of liquid, the liquid point sample of picoliters rank is realized with this.
The present invention efficiently solves the sample-adding difficult point that existing micro sample adding appliance is directed to ultra micro quantity of fluid, it is possible to achieve picoliters level
Other sample-adding, effectively improves the utilization rate of micro valuable sample.
Brief description of the drawings
Fig. 1 is the structural diagrams of ultra micro quantity of fluid sample injector of the present invention.
Fig. 2 is the structural diagrams of the array sample injector of the present invention.
Fig. 3 is capillary micro-imaging figure.
Fig. 4 is drop figure.
Label in figure：1 is heat conductive filament, and 2 be heating source, and 3 be copper wire, and 4 be capillary, and 5 be drop, and 6 be heating electrodes, 7
For sample stage.
Embodiment
Presently preferred embodiments of the present invention is described in detail below in conjunction with the accompanying drawings, so that advantages and features of the invention energy
It is more easy to be readily appreciated by one skilled in the art, apparent is clearly defined so as to be made to protection scope of the present invention.
Embodiment 1：
Step 1, prepare capillary, capillary inner diameter is 1 micron, and external diameter is 3 microns；Capillary is glass tube；The side of capillary
Wall opens an inlet at 2/3rds height；
Copper wire is placed in step 2, capillary, copper wire is located at the middle rear end in capillary（It is top in figure）At about 2/3rds；
Step 3, by micro liquid pump micro liquid is pumped into capillary from capillary left hand inlet port；
Step 4, regulation heating source temperature, copper wire is reached by heat conductive filament by heat；
Step 5, liquid is added dropwise by required container by the thermal expansion of copper wire, each of the droplets size is about 0.1 picoliters.
Embodiment 2：
Step 1, the array sample injector for preparing multiple capillaries compositions, capillary inner diameter control is in 1-10 microns, external diameter control
In 2-20 microns；Each capillary is made up of glass tube；One is opened respectively at 2/3rds height in the side wall of each capillary to enter
Liquid mouthful；
Copper wire is placed in step 2, each capillary, copper wire is located at the middle rear end in capillary（It is top in figure）About 2/3rds
Place；
Step 3, by micro liquid pump micro liquid is pumped into capillary from capillary left hand inlet port,
Step 4, regulation heating electrodes temperature, copper wire is reached by heat conductive filament by heat；
Step 5, the XYZ three-axis movings by computer control sample stage, required position is moved to by sample stage；
Step 6, liquid is added dropwise by required container by the thermal expansion of copper wire, realizes the sample-adding of picoliters rank.
Claims (4)
1. a kind of ultra micro quantity of fluid sample injector, it is characterised in that structure composition includes：
One internal diameter is that in 1-10 microns, external diameter is the capillary in 2-20 microns；Capillary is made up of heat-insulating material；Capillary
Side wall upper part is provided with inlet；
One miniature heat transfer device for being positioned in capillary；
One heating source for being connected with miniature heat transfer device, for heating miniature heat transfer device；
One micro liquid pump, for ultra micro quantity of fluid to be pumped into capillary from inlet.
2. ultra micro quantity of fluid sample injector according to claim 1, it is characterised in that the miniature heat transfer device is copper wire.
3. ultra micro quantity of fluid sample injector according to claim 1, it is characterised in that the capillary has multiple, constitutes battle array
Row sample injector.
4. the ultra micro quantity of fluid sample injector according to claim 1 or 3, it is characterised in that operating process when using is：By
Micro liquid is pumped into capillary by micro liquid pump；Regulation heating source temperature, miniature heat conduction is reached by heating unit by heat
Device；Miniature heat transfer device expanded by heating, required container is added dropwise by micro liquid from capillary drip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710448968.4A CN107199062B (en) | 2017-06-14 | 2017-06-14 | Ultra-micro liquid sample injector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710448968.4A CN107199062B (en) | 2017-06-14 | 2017-06-14 | Ultra-micro liquid sample injector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107199062A true CN107199062A (en) | 2017-09-26 |
| CN107199062B CN107199062B (en) | 2020-01-10 |
Family
ID=59908050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710448968.4A Active CN107199062B (en) | 2017-06-14 | 2017-06-14 | Ultra-micro liquid sample injector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107199062B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87203080U (en) * | 1987-03-23 | 1988-02-17 | 张玉斌 | Precision pipet |
| US20110182782A1 (en) * | 2010-01-22 | 2011-07-28 | Tecan Trading Ag | Positive displacement pump with pressure sensor |
| CN103971978A (en) * | 2014-04-12 | 2014-08-06 | 北京工业大学 | Thermal expansion liquid contact microswitch for heating through induction |
| CN106540757A (en) * | 2015-09-21 | 2017-03-29 | 中国科学院大连化学物理研究所 | A kind of magnetically-actuated liquid quantitative actuation means |
-
2017
- 2017-06-14 CN CN201710448968.4A patent/CN107199062B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87203080U (en) * | 1987-03-23 | 1988-02-17 | 张玉斌 | Precision pipet |
| US20110182782A1 (en) * | 2010-01-22 | 2011-07-28 | Tecan Trading Ag | Positive displacement pump with pressure sensor |
| CN103971978A (en) * | 2014-04-12 | 2014-08-06 | 北京工业大学 | Thermal expansion liquid contact microswitch for heating through induction |
| CN106540757A (en) * | 2015-09-21 | 2017-03-29 | 中国科学院大连化学物理研究所 | A kind of magnetically-actuated liquid quantitative actuation means |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107199062B (en) | 2020-01-10 |
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