CN107192815A - A kind of antibody incubation kit and its application method - Google Patents
A kind of antibody incubation kit and its application method Download PDFInfo
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- CN107192815A CN107192815A CN201710442993.1A CN201710442993A CN107192815A CN 107192815 A CN107192815 A CN 107192815A CN 201710442993 A CN201710442993 A CN 201710442993A CN 107192815 A CN107192815 A CN 107192815A
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- groove
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- silicon rubber
- antibody incubation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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Abstract
The invention discloses a kind of antibody incubation kit and its application method, the antibody incubation kit, comprising box body and lid, the box body includes silicon rubber, waterproof film, wet tissue and groove；Silicon rubber is housed, waterproof film is laid on silicon rubber, sets fluted on waterproof film, the inwall of box body is with wet tissue in the box body.Application method is：The groove less than silicon rubber plane is produced on the waterproof film for being laid in silicon rubber, hybond membrane is carried out after transferring film, rinsing, hybond membrane protein-contg one is upwardly, it is horizontally arranged in groove, antibody diluent is added dropwise, hybond membrane is infiltrated in antibody diluent, hybridization reaction is carried out.The present invention antibody incubation kit application method it is simple to operate, effect is good, cost is low.
Description
Technical field
The invention belongs to experimental apparatus field, and in particular to a kind of antibody incubation kit and its application method.
Background technology
Commonly using the inspection of the hybridization blot assays such as western blotting, southern blotting in Bioexperiment
Survey purpose band.The incubation process of hybridization, conventional method is that the film where molecule is fully immersed in the liquid containing antibody,
By>The time of 1 hour causes antibody to be combined with the molecules of interest on film.
In Western blotting, typically film and antibody are placed in capsule, are incubated on shaking table, the method is required every time
Amount of antibody it is larger；It is difficult to reply multispecific antibody to be incubated；Antibody reclaims incomplete, different according to the material of capsule, the efficiency of recovery
It is not quite similar.The method of current another antibody incubation, is that film and antibody are placed in the pouch that plastic packaging film is made, the method exists
Sample-adding and antibody cause a large amount of wastes of time and antibody when reclaiming, and are difficult to remove the bubble for having extreme influence to experiment；
Plastic packaging film is disposably used in addition, the wasting of resources and environmental pollution is caused.
Therefore it is necessary that a kind of convenient and swift, effect stability and cost-effective kit and its user are provided
Method.
The content of the invention
It is an object of the invention to provide a kind of antibody incubation kit that simple to operate, effect is good, cost is low and its make
Use method.
It is described comprising box body and lid the invention provides a kind of antibody incubation kit in order to realize the object of the invention
Box body includes silicon rubber, waterproof film, wet tissue and groove；Silicon rubber is housed, waterproof film is laid in silicon in the box body
On rubber, set fluted on waterproof film, the inwall of box body is with wet tissue.
Further, the silicon rubber is Polymethyl methacrylate.
Further, thickness >=5mm of the silicon rubber.
Further, the waterproof film is masking foil, preservative film or sealed membrane.
Further, the quantity of the groove is more than one.
Further, the groove is polygon, circular or irregular shape；Preferably, the groove is to agree with hybond membrane
Polygon, circular or irregular shape.
Present invention also offers a kind of application method of above-mentioned antibody incubation kit：It is being laid in the waterproof of silicon rubber
The groove less than silicon rubber plane is produced on film, by hybond membrane carry out transferring film, rinsing after, hybond membrane protein-contg one towards
On, it is another towards bottom portion of groove, it is horizontally arranged in groove, antibody diluent is added dropwise, hybond membrane is infiltrated in antibody diluent,
Carry out hybridization reaction.
Further, the groove is formed by applying certain pressure on waterproof film, makes waterproof film and groove
It is brought into close contact, the depth of groove is 2-3mm, and groove area is more than the area of hybond membrane.
Further, the addition of the antibody diluent is 3~4mL antibody diluents/(6cm × 8cm) hybond membrane.
Further, the hybond membrane is pvdf membrane or NC films.
Generally, experiment is incubated 6cm × 8cm hybond membranes using capsule needs more than 10ml antibody diluents, the present invention
The addition of antibody diluent is 3~4mL antibody diluents/(6cm × 8cm) hybond membrane, greatly reduces antibody consumption, effectively
Reagent cost is saved.
Antibody incubation kit of the present invention is with a circle wet tissue in inboard wall of cartridge, can effectively prevent the steaming of dilution
Hair.
The present invention using silicon rubber mobility and plasticity, make to be laid in the waterproof thin film fabrication on silicon rubber go out it is low
In the groove of silicon rubber plane, for providing space to antibody incubation.
Kit simple and effective of the present invention, application method is simple, can be arranged as required to multiple grooves, carries out simultaneously
The incubation of different antibodies.Groove can make according to the size and shape of hybond membrane, can be circular, polygon or irregular figure,
The space formed is minimum, the administration of just right probe dilution liquid, is greatly saved amount of antibody.
When being incubated Multiple Antibodies, in silicon rubber plane that can be in same seal box, multiple grooves are made, are entered simultaneously
Row experiment.Antibody is only stored in groove, no cross contamination.Shaking table can be placed to be incubated to obtain more preferable crossbreeding effect.
Certain groove due to foring, when being incubated on shaking table, antibody diluent will not flow out, and be incubated effect good.
Groove is easy to the hydrophobicity of the direct recovery, in addition waterproof film of antibody, therefore the antibody kit of the present invention
Antibody diluent the rate of recovery it is good, up to 99%.
The application method of antibody incubation kit of the present invention with hermetic bag be incubated antibody method compared with, take pvdf membrane and
Reclaim antibody more convenient and complete, save more than twice of time cost and reagent cost.After use all utensils and
Material rear recycling capable of washing.
Antibody incubation kit of the present invention can be used for the traces such as western blotting, southern blotting miscellaneous
Hand over experiment.
Brief description of the drawings
Fig. 1 is kit top view；
Wherein：Box body 1, silicon rubber 2, wet tissue 3, waterproof film 4, groove 5, hybond membrane 6.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is intended to be used to explain the present invention, and can not
It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, according to described by document in the art
Technology or condition or carried out according to product description.Agents useful for same or the unreceipted production firm person of instrument, are to lead to
Cross the conventional products of acquisition purchased in market.
The preparation of the antibody kit of embodiment 1
1st, a sealable box is taken, makes to form a water in box body 1 in the silicon rubber 2 of its laid inside 5mm thickness
Plane.
2nd, the waterproof film 4 that length and width is slightly larger than pvdf membrane 6 is cut, waterproof film 4 silicon rubber 2 is arranged in flat
Suitable position tiling on face.Using the plasticity of Polymethyl methacrylate, apply to the waterproof Middle face of film 4 and press
Power so that bottom surface sinking 2-3mm.So far the waterproof bottom of film 4 forms groove 5, groove 5 and waterproof film 4 are tight into plane
It is closely connected to close.
3rd, made a circle wet tissue 3 in seal box inwall, to prevent the evaporation of antibody diluent.
Waterproof film can be sealed membrane, preservative film or masking foil in embodiment.
Silicon rubber is Polymethyl methacrylate in embodiment.
The use of the antibody kit of embodiment 2
By taking western blotting as an example：
The transferring film of hybond membrane 6, it is rinsed after, by pvdf membrane containing albumen one upwardly, be horizontally arranged in the kit of embodiment 1
Groove 5 in, antibody diluent is directly added dropwise.The addition of antibody diluent is 3~4mL antibody diluents/(6cm × 8cm)
Hybond membrane.The volume of antibody diluent not should be greater than groove 5.Hybond membrane 6 is set to be infiltrated in antibody diluent, progress hybridizes anti-
Should.Incubation process can keep horizontal positioned, can also slowly be shaken with low-angle.
Antibody is reclaimed：Lift one jiao to take away after hybond membrane, antibody-solutions are resided in the groove 5 of the waterproof formation of film 4,
Directly can quickly it be drawn using liquid-transfering gun.
Hybond membrane is pvdf membrane or NC films in embodiment.
It the experiment proved that, in the case of without causing antibody to be lost in because of misoperation, time of the antibody diluent of the method
Yield is up to 99%.Recycled after utensil used in the method and material are capable of washing, save consumables cost.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from the principle and objective of the present invention
In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.
Claims (10)
1. a kind of antibody incubation kit, includes box body and lid, it is characterised in that the box body includes silicon rubber (2), impermeable
Water film (4), wet tissue (3) and groove (5)；Silicon rubber (2) is housed, waterproof film (4) is laid in silicon rubber in the box body (1)
On glue (2), set fluted (5) on waterproof film (4), the inwall of box body (1) is with wet tissue (3).
2. antibody incubation kit according to claim 1, it is characterised in that the silicon rubber (2) is poly- methyl ethylene
Siloxanes.
3. antibody incubation kit according to claim 1, it is characterised in that thickness >=5mm of the silicon rubber (2).
4. antibody incubation kit according to claim 1, it is characterised in that the waterproof film (4) is masking foil, protected
Fresh film or sealed membrane.
5. antibody incubation kit according to claim 1, it is characterised in that the quantity of the groove (5) is more than one.
It is circular or not 6. antibody incubation kit according to claim 1, it is characterised in that the groove (5) is polygon
Regular shape；Preferably, the groove (5) is the polygon for agreeing with hybond membrane (6), circular or irregular shape.
7. the application method of any one of a kind of claim 1-6 antibody incubation kits, it is characterised in that be laid in silicon
The groove (5) less than silicon rubber (2) plane is produced on the waterproof film (4) of rubber (2), by hybond membrane (6) carry out transferring film,
After rinsing, hybond membrane (6) protein-contg one is upwardly, another towards bottom portion of groove (5), is horizontally arranged in groove (5), is added dropwise anti-
Body dilution, makes hybond membrane (6) be infiltrated in antibody diluent, carries out hybridization reaction.
8. the application method of antibody incubation kit according to claim 7, it is characterised in that the groove (5) by
Apply certain pressure on waterproof film (4) to be formed, waterproof film (4) is brought into close contact with groove, the depth of groove (5) is
2-3mm, groove (5) area is more than the area of hybond membrane (6).
9. the application method of antibody incubation kit according to claim 7, it is characterised in that the antibody diluent adds
Dosage is：Per 6cm × 8cm hybond membranes addition 3~4mL antibody diluents.
10. according to the application method of any one of the claim 7-9 antibody incubation kits, it is characterised in that the hybridization
Film is pvdf membrane or NC films.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710442993.1A CN107192815B (en) | 2017-06-13 | 2017-06-13 | A kind of antibody incubation kit and its application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710442993.1A CN107192815B (en) | 2017-06-13 | 2017-06-13 | A kind of antibody incubation kit and its application method |
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| Publication Number | Publication Date |
|---|---|
| CN107192815A true CN107192815A (en) | 2017-09-22 |
| CN107192815B CN107192815B (en) | 2019-06-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710442993.1A Active CN107192815B (en) | 2017-06-13 | 2017-06-13 | A kind of antibody incubation kit and its application method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204422535U (en) * | 2015-01-13 | 2015-06-24 | 河南赛诺特生物技术有限公司 | A kind of novel agent box possessing the immunohistochemical reagents of moisture-keeping functions |
| CN205620419U (en) * | 2016-02-16 | 2016-10-05 | 张新化 | Western blotting determine module |
| CN106093372A (en) * | 2016-06-07 | 2016-11-09 | 遵义医学院 | A kind of western blot antibody incubation box |
| CN206103954U (en) * | 2016-10-14 | 2017-04-19 | 湖州数康生物科技有限公司 | Biochip box that high flux jointly detected or filtered based on silica gel matrix |
| CN106610429A (en) * | 2015-10-26 | 2017-05-03 | 宁波大学 | Dual-drive tumor marker joint inspection chip device with PDMS as substrate |
-
2017
- 2017-06-13 CN CN201710442993.1A patent/CN107192815B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204422535U (en) * | 2015-01-13 | 2015-06-24 | 河南赛诺特生物技术有限公司 | A kind of novel agent box possessing the immunohistochemical reagents of moisture-keeping functions |
| CN106610429A (en) * | 2015-10-26 | 2017-05-03 | 宁波大学 | Dual-drive tumor marker joint inspection chip device with PDMS as substrate |
| CN205620419U (en) * | 2016-02-16 | 2016-10-05 | 张新化 | Western blotting determine module |
| CN106093372A (en) * | 2016-06-07 | 2016-11-09 | 遵义医学院 | A kind of western blot antibody incubation box |
| CN206103954U (en) * | 2016-10-14 | 2017-04-19 | 湖州数康生物科技有限公司 | Biochip box that high flux jointly detected or filtered based on silica gel matrix |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107192815B (en) | 2019-06-11 |
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