CN107192713A - A kind of automatic micro imaging method of space science experimentation - Google Patents

A kind of automatic micro imaging method of space science experimentation Download PDF

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Publication number
CN107192713A
CN107192713A CN201710387541.8A CN201710387541A CN107192713A CN 107192713 A CN107192713 A CN 107192713A CN 201710387541 A CN201710387541 A CN 201710387541A CN 107192713 A CN107192713 A CN 107192713A
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CN
China
Prior art keywords
micro
image
space science
imaging method
camera
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Pending
Application number
CN201710387541.8A
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Chinese (zh)
Inventor
郑伟波
张涛
张寅�
董栋
孙浩
郑行龙
童广辉
袁永春
刘方武
顾建忠
谢焘
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Shanghai Institute of Technical Physics of CAS
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Shanghai Institute of Technical Physics of CAS
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Priority to CN201710387541.8A priority Critical patent/CN107192713A/en
Publication of CN107192713A publication Critical patent/CN107192713A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications

Abstract

Test automatic micro imaging method and system the invention discloses a kind of space science, method based on device include low-power consumption lighting source microcobjective, image detector, ball screw and stepper motor;Target sample is irradiated to by low-power consumption lighting source, micro- camera obtains the image of every aspect in test unit by precision translation stages, method is adjudicated according to image definition, determine the position where target sample, then micro- camera is moved to the position, the micro-image of target sample is obtained, and there is provided analyzed and researched to scientist by passing passage down-transmitting data under limited satellite.The system has the advantages that low-power consumption, high reliability and automatic acquisition target micro-image, it is adaptable to which space science experimentation obtains the micro-image of the micro-meter scale target of space three-dimensional distribution.

Description

A kind of automatic micro imaging method of space science experimentation
Technical field
Field, more particularly to a kind of space science experimentation are observed the invention belongs to the detection of space science experimentation Automatic micro imaging method.
Background technology
Space science experiment needs highly reliable, full automatic instrument and equipment, obtains the image letter of in-orbit scientific experiment process Breath, particularly microscopic image information, carry out analysis and research significant for scientist.Traditional light microscope volume It is huge, heavy, it is necessary to professional human users, it is impossible to meet mechanical environment condition when flight transmitting is returned and in-orbit flew Journey automatically obtains the requirement of target micro-image.Satellite down-transmitting data bandwidth is limited simultaneously, and effective target can only be passed down and is shown Micro- view data, needs to research and develop the automatic micro-imaging equipment for space science experimentation for this.
The content of the invention
To solve the above problems, the present invention provides a kind of automatic acquisition space science experimentation available for satellite platform The method of micro-image.
The present invention provides a kind of space science and tests automatic micro imaging method, comprises the following steps:A1. lighting source shines It is mapped in target sample;A2. micro- camera obtains image by microcobjective and image detector;A3. micro- camera passes through essence Close mobile station obtains the image of every aspect on depth direction;A4. micro- camera adjudicates method according to image definition function, obtains Aspect where target;A5. micro- camera is moved to the aspect, image is obtained again, and pass through blit picture under satellite data transmission interface.
The present invention also provides a kind of space science and tests automatic micro imaging system, including low-power consumption lighting source, micro- Object lens, image detector, precision translation stages;Described low-power consumption lighting source is 0.1W, the white light LEDs that 15 ° of lighting angle;Institute The microcobjective stated is connected with micro- camera by C interface;Described micro- camera is fastened on precision translation stages by screw.
Described lighting source isRated voltage 3.3V, rated current 30mA, the white light LEDs that 15 ° of lighting angle Light source.
Described microcobjective can select 4 times, 10 times or 20 times enlargement ratios, object space working distance>5mm.
Described image detector is the cmos detector of 1280 × 1024 pixels, and Pixel Dimensions are 6.7um × 6.7um.
Described precision translation stages are made up of ball-screw translation platform and stepper motor, and stepping accuracy is micron order, ball Screw lead is 1mm, and stroke is 10mm, and stepper motor step angle is 0.9 °.
Every aspect image is the step of stepping 600 on described depth direction, obtains 600 layer images.
Described image definition judgement method solves object pixel and the maximum difference value of adjacent pixels to face domain difference.
Described satellite data transmission interface is 422 interfaces, and baud rate is 115200bps.
Beneficial effects of the present invention are:Target sample is irradiated to by low-power consumption lighting source, micro- camera passes through precision Mobile station obtains the image of every aspect in culture unit, adjudicates method according to image definition, determines the position where target sample Put, then move micro- camera to the position, obtain the micro-image of target sample, and by being passed under limited satellite under resource Passing data, there is provided analyzed and researched to scientist.The system has low-power consumption, high reliability and automatic acquisition target micrograph The advantage of picture, it is adaptable to which space science experimentation obtains the micro-meter scale target of space three-dimensional distribution, such as cell, tissue etc. Micro-image.
Brief description of the drawings
Fig. 1 tests the composition schematic diagram of automatic micro imaging system for the space science of the embodiment of the present invention.
Fig. 2 is the distribution schematic diagram of target sample of the embodiment of the present invention.
Fig. 3 is the schematic diagram of imaging process of the embodiment of the present invention.
Embodiment
With reference to embodiment, and compare accompanying drawing the present invention be described in further detail, it should be emphasised that, What the description below was merely exemplary, the scope being not intended to be limiting of the invention and its application.
The present embodiment provides a kind of space science and tests automatic micro imaging method, comprises the following steps:A1. lighting source It is irradiated in target sample;A2. micro- camera obtains image by microcobjective and image detector;A3. micro- camera passes through Precision translation stages obtain the image of 600 aspects on depth direction;A4. micro- camera adjudicates method according to image definition function, Obtain aspect where target;A5. micro- camera is moved to the aspect, and image is obtained again, and by being passed under satellite data transmission interface Image.
Embodiment
As shown in figure 1, a kind of space science tests automatic micro imaging system, including low-power consumption lighting source 1, micro- thing Mirror 2, image detector 3, ball screw 4, stepper motor 5 and target sample 6.
Lighting source isRated voltage 3.3V, rated current 30mA, the white LED light source that 15 ° of lighting angle, By lighting source switch and time for exposure match control, power consumption can be further reduced.
Microcobjective is using the microcobjective of 4 times, 10 times or 20 times enlargement ratios of standard C interface, object space working distance> 5mm, is so adapted to different scale, the demand of different enlargement ratio imagings, and be adapted to test unit with certain The demand of wall thickness and depth.
Image detector is the cmos detector of 1280 × 1024 pixels, and Pixel Dimensions are 6.7um × 6.7um, if choosing With 10 or 20 object lens, it is possible to achieve the micro-imaging of micrometer resolution.Microcobjective and image detector pass through standard C interface Connection, by reinforcing, it is ensured that being connected for relative position, can adapt to satellite launch and the mechanical environment condition returned.
Precision translation stages are made up of ball-screw translation platform and stepper motor, and stepping accuracy is micron order, and ball screw is led Journey is 1mm, and stroke is 10mm, and stepper motor step angle is 0.9 °, and stepper motor often walks a step angle, and ball screw is advanced 2.5um, by controlling the order that stepper motor is encouraged to realize the rotating of stepper motor, that is, realizes the advance of ball screw with after Move back.Micro- camera is fastened on precision translation stages by screw, then by motor control, realizes that the micron order of micro- camera advances And retrogressing.
As shown in Fig. 2 the small objects such as cell or tissue sample 10 is loaded in by culture box 7, permeable membrane 8 and watch window In the culture unit of 9 compositions, culture unit is that target sample (cell or tissue) provides nutrient solution prescription, gas component, temperature With raw guarantor's condition such as pH value, in incubation, the change of microexamination target sample can be carried out by watch window 9 Journey.
During imaging, low-power consumption lighting source is irradiated to target sample, and it is single that micro- camera obtains culture by precision translation stages In first, as shown in figure 3, obtaining the image (n=599) of m, m+1, m+n every aspect, method is adjudicated according to image definition, it is determined that Position where target sample, then moves micro- camera to the position, obtains the micro-image of target sample, and pass through 422 Interface is passed under limited satellite to be passed view data under resource there is provided analyzed and researched to scientist.

Claims (6)

1. a kind of space science tests automatic micro imaging method and system, it is characterised in that comprise the following steps:
1) lighting source is irradiated in target sample i.e. space science experimentation on the cell or tissue of dimensional culture;
2) micro- camera obtains image by microcobjective and image detector;
3) micro- camera obtains the image of 600 aspects on depth direction by precision translation stages;
4) micro- camera adjudicates method according to image definition function, obtains aspect where target;
5) micro- camera is moved to the aspect, image is obtained again, and pass through blit picture under satellite data transmission interface.
2. a kind of space science experiment micro imaging method as claimed in claim 1, it is characterised in that described lighting source For low-power consumption 0.1W white LED light sources.
3. a kind of space science as claimed in claim 1 tests automatic micro imaging method and system, it is characterised in that described Micro- camera include microcobjective and image detector, described image detector detects for the CMOS of 1280 × 1024 pixels Device, Pixel Dimensions are 6.7um × 6.7um.
4. a kind of space science as claimed in claim 1 tests automatic micro imaging method and system, it is characterised in that described Precision translation stages be made up of ball-screw translation platform and stepper motor, ball screw helical pitch be 1mm, stroke is 10mm, stepping Motor step angle is 0.9 °, and stepping accuracy is micron order.
5. a kind of space science as claimed in claim 1 tests automatic micro imaging method and system, it is characterised in that described Image definition judgement method to face domain difference, solve object pixel and the maximum difference value of adjacent pixels.
6. a kind of space science as claimed in claim 1 tests automatic micro imaging method and system, it is characterised in that described Satellite data transmission interface be 422 interfaces, baud rate is 115200bps.
CN201710387541.8A 2017-05-27 2017-05-27 A kind of automatic micro imaging method of space science experimentation Pending CN107192713A (en)

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Application Number Priority Date Filing Date Title
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CN107192713A true CN107192713A (en) 2017-09-22

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1563947A (en) * 2004-03-18 2005-01-12 中国科学院上海技术物理研究所 High microspectrum imaging system
CN101099104A (en) * 2004-11-24 2008-01-02 巴特尔纪念研究所 Optical system for cell imaging
CN101788709A (en) * 2010-03-10 2010-07-28 广西大学 Digital confocal microscope optical section collecting device
US20120194669A1 (en) * 2011-01-27 2012-08-02 Hutto Kevin W Fluid sample analysis systems

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1563947A (en) * 2004-03-18 2005-01-12 中国科学院上海技术物理研究所 High microspectrum imaging system
CN101099104A (en) * 2004-11-24 2008-01-02 巴特尔纪念研究所 Optical system for cell imaging
CN101788709A (en) * 2010-03-10 2010-07-28 广西大学 Digital confocal microscope optical section collecting device
US20120194669A1 (en) * 2011-01-27 2012-08-02 Hutto Kevin W Fluid sample analysis systems

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
郑行龙 等: "空间科学实验中三维立体目标显微信息获取技术", 《半导体光电》 *

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Application publication date: 20170922