CN107192711A - 一种通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法 - Google Patents
一种通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法 Download PDFInfo
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Abstract
本发明涉及一种通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,配制赖氨酸修饰的苝二酰亚胺溶液;制备赖氨酸修饰的苝二酰亚胺功能化银(LPL‑AgNPs)纳米颗粒:向配置好的赖氨酸修饰的苝二酰亚胺中加入硝酸银,搅拌过程中加入硼氢化钠,继续搅拌;Cd2+、Pb2+离子的检测:向制备好的LPL‑AgNPs中加入重金属离子溶液,观察LPL‑AgNPs溶液颜色变化。本发明的制备方法简单,使用方便,检测限低,解决了传统检测手段耗时长、成本高的问题。
Description
技术领域
本发明涉及纳米材料技术领域,具体涉及一种通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法。
背景技术
纳米材料因其许多独特的性能,如表面与界面效应、小尺寸效应、隧穿效应、量子尺寸效应,而一直是科学家们关注的焦点。也因为这些独特的性能使得纳米材料在众多领域都有广泛的应用。
近些年来,以金属纳米颗粒作为可视化检测重金属离子得到了深入的研究。通过不同分子功能化后可以得到对不同金属离子具有特异性检测的金属纳米粒子溶胶。该类方法相比传统的仪器分析方法更为简单,价格低廉,快速方便。本发明中使用一种以赖氨酸修饰的苝二酰亚胺作为修饰剂检测Cd2+、Pb2+离子,得到了良好的使用结果。
发明内容
本发明公开一种以赖氨酸修饰的苝二酰亚胺功能化银纳米颗粒(LPL-AgNPs)作为定性检测Cd2+、Pb2+离子的新型可视化检测试剂的方法,该方法解决了传统检测手段耗时长、成本高的问题。
本发明提供的技术方案是:
一种通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,包括以下步骤:
1)配制赖氨酸修饰的苝二酰亚胺溶液;
赖氨酸修饰的苝二酰亚胺分子结构式:
2)制备赖氨酸修饰的苝二酰亚胺功能化银(LPL-AgNPs)纳米颗粒:向配置好的赖氨酸修饰的苝二酰亚胺中加入硝酸银,搅拌过程中加入硼氢化钠,继续搅拌;
3)Cd2+、Pb2+离子的检测:向制备好的LPL-AgNPs中加入重金属离子溶液,观察LPL-AgNPs溶液颜色变化。
步骤1)中,LPL-AgNPs配制pH为11、12。
步骤2)中,所述硝酸银溶液浓度为0.01M,所述硼氢化钠溶液浓度为0.1M。
步骤2)中,所述赖氨酸修饰的苝二酰亚胺溶液与硝酸银溶液、硼氢化钠溶液的体积比为 40:1:1。
步骤2)中,加入硝酸银后搅拌溶液5min,加入硼氢化钠后搅拌溶液1min,反应的环境温度为20℃~30℃。
步骤3)中,LPL-AgNPs与重金属离子溶液体积比为4:1。
LPL-AgNPs对Cd2+、Pb2+的识别机理在于功能化试剂赖氨酸修饰的苝二酰亚胺与Cd2 +、 Pb2+络合,使得纳米银离子发生聚集,进而导致纳米银溶液颜色发生变化。LPL-AgNPs与重金属离子溶液体积比为4:1。
有益效果:
本发明中的赖氨酸修饰的苝二酰亚胺功能化银纳米颗粒制备方法简单,使用方便,检测限低,可以在5分钟之内检测到水体系中的Cd2+、Pb2+离子。
附图说明
图1:a~e分别为LPL-AgNPs加入Cd2+20μM、Cd2+10μM,Pb2+20μM、Pb2+5μM、 Pb2+2μM后的TEM图片。
具体实施方式
下面结合具体实施例对本发明作进一步说明。
实施例1
(1)pH=11时氨酸修饰的苝二酰亚胺功能化银纳米溶液(LPL-AgNPs)的制备:移取16mL pH=11的上述溶液(7.5μM),加入0.01M硝酸银0.1mL,搅拌5min,再加入0.10M硼氢化钠0.1mL,搅拌1min。
(2)pH=11的赖氨酸修饰的苝二酰亚胺功能化银纳米颗粒对不同金属离子的检测:向制备好的LP-AgNPs中加入分别加入Cr2O7 2-、Mn2+、Cd2+、Pb2+、Ba2+、Al3+、Zn2+、Ca2+、Na+、K+溶液,并使金属离子最终浓度为10μM,5min后可以看到加入Cd2+的LPL-AgNPs颜色发生了明显的变化,由黄色变为暗黄色。观察LPL-AgNPs溶液的颜色变化。
(3)pH=10时赖氨酸修饰的苝二酰亚胺功能化纳米银溶液对不同浓度的Cd2+离子的检测:向制备好的LPL-AgNPs溶液中分别加入Cd2+(20μM、10μM、5μM、2μM、1μM、500nM、200nM、150nM、100nM、50nM、20nM溶液,发现除了加入20、10μM的LPL-AgNPs颜色发生变化外,其他AgNPs颜色均无变化。观察溶液颜色的变化。
本发明操作过程简单,使用方便,对水体系中Cd2+、Pb2+有着很好的定性识别作用。
以上所述,仅是本发明的较佳实施例,并非对本发明作任何形式上的限制,任何熟悉本专业的技术人员,在不脱离本发明技术方案范围内,依据本发明的技术实质,对以上实施例所作的任何简单的修改、等同替换与改进等,均仍属于本发明技术方案的保护范围之内。
Claims (6)
1.一种通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,其特征在于:包括以下步骤:
1)配制赖氨酸修饰的苝二酰亚胺溶液;
2)制备赖氨酸修饰的苝二酰亚胺功能化银(LPL-AgNPs)纳米颗粒:向配置好的赖氨酸修饰的苝二酰亚胺中加入硝酸银,搅拌过程中加入硼氢化钠,继续搅拌;
3)Cd2+、Pb2+离子的检测:向制备好的LPL-AgNPs中加入重金属离子溶液,观察LPL-AgNPs溶液颜色变化。
2.根据权利要求1所述的通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,其特征在于:步骤1)中,LPL-AgNPs配制pH为11、12。
3.根据权利要求1所述的通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,其特征在于:步骤2)中,所述硝酸银溶液浓度为0.01M,所述硼氢化钠溶液浓度为0.1M。
4.根据权利要求1所述的通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,其特征在于:步骤2)中,所述赖氨酸修饰的苝二酰亚胺溶液与硝酸银溶液、硼氢化钠溶液的体积比为40:1:1。
5.根据权利要求1所述的通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,其特征在于:步骤2)中,加入硝酸银后搅拌溶液5min,加入硼氢化钠后搅拌溶液1min,反应的环境温度为20℃~30℃。
6.根据权利要求1所述的通过荧光分子修饰的银溶胶检测Cd2+、Pb2+离子的方法,其特征在于:步骤3)中,LPL-AgNPs与重金属离子溶液体积比为4:1。
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CN112851672A (zh) * | 2021-01-19 | 2021-05-28 | 河南大学 | “on-off-on”型连续性检测的荧光探针及其制备方法和应用 |
CN114309581A (zh) * | 2021-12-30 | 2022-04-12 | 山东大学 | 银离子修饰的银溶胶及其制备方法以及在表面增强拉曼光谱定量检测砷中的应用 |
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CN103160504A (zh) * | 2013-03-01 | 2013-06-19 | 上海交通大学 | 用于重金属铅的快速检测的核酸荧光探针及其检测方法 |
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CN112851672A (zh) * | 2021-01-19 | 2021-05-28 | 河南大学 | “on-off-on”型连续性检测的荧光探针及其制备方法和应用 |
CN114309581A (zh) * | 2021-12-30 | 2022-04-12 | 山东大学 | 银离子修饰的银溶胶及其制备方法以及在表面增强拉曼光谱定量检测砷中的应用 |
CN114309581B (zh) * | 2021-12-30 | 2022-08-30 | 山东大学 | 银离子修饰的银溶胶及其制备方法以及在表面增强拉曼光谱定量检测砷中的应用 |
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