CN107192657A - A kind of algae on-line detecting system - Google Patents
A kind of algae on-line detecting system Download PDFInfo
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- CN107192657A CN107192657A CN201710513180.7A CN201710513180A CN107192657A CN 107192657 A CN107192657 A CN 107192657A CN 201710513180 A CN201710513180 A CN 201710513180A CN 107192657 A CN107192657 A CN 107192657A
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 86
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 234
- 238000003760 magnetic stirring Methods 0.000 claims abstract description 25
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 22
- 238000005070 sampling Methods 0.000 claims description 20
- 238000002347 injection Methods 0.000 claims description 16
- 239000007924 injection Substances 0.000 claims description 16
- 238000002604 ultrasonography Methods 0.000 claims description 5
- 241000009298 Trigla lyra Species 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 238000001514 detection method Methods 0.000 abstract description 27
- 238000011065 in-situ storage Methods 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 13
- 238000012544 monitoring process Methods 0.000 description 11
- 239000012530 fluid Substances 0.000 description 8
- 238000007689 inspection Methods 0.000 description 8
- 230000004087 circulation Effects 0.000 description 5
- 230000005611 electricity Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000000684 flow cytometry Methods 0.000 description 5
- 239000005416 organic matter Substances 0.000 description 5
- 238000002203 pretreatment Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 241000192700 Cyanobacteria Species 0.000 description 3
- 241000192710 Microcystis aeruginosa Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000007667 floating Methods 0.000 description 3
- 230000009183 running Effects 0.000 description 3
- 238000013019 agitation Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000005446 dissolved organic matter Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of algae on-line detecting system, the pond for being connected with the water inlet pipe of multiple water inlet pipes including one end and being attached thereto, it is connected with magnetic valve and water pump on the water inlet pipe in turn, the bottom in the pond is provided with magnetic stirring apparatus, the pond side is provided with ultrasonic cell disintegration instrument, the ultrasonic cell disintegration instrument is connected by cable with one end of ultrasonic transformer, the other end of the ultrasonic transformer is stretched into the pond, the pond is built with level sensor, the pond is connected by water pipe with CytoSense flow cytometers, the sidewall bottom in the pond is connected with drainpipe, the side wall upper part in the pond is connected with overflow pipe；The magnetic stirring apparatus, ultrasonic cell disintegration instrument, CytoSense flow cytometers, level sensor, magnetic valve, peristaltic pump are electrically connected with electronic control box respectively, and the electronic control box is electrically connected with terminal computer.The system have the advantages that detection efficiency it is high, can real-time in-situ detection, testing result it is accurate, applied widely.
Description
Technical field
The invention belongs to algae detection technique field, and in particular to a kind of algae on-line detecting system.
Background technology
Want to carry out accurate early warning before breakout of cyanobacteria blooms, it is conscientiously may be used to continuously monitor blue-green algae content in water
Capable approach.Current continuous monitoring relies primarily on flow cytometer to monitor analysis, the general principle of flow cytomery
For：The particle (including cell) allowed according to principle of hydrodynamics in liquid passes through laser beam (detection zone), laser irradiation one by one
The scattering of light can be caused on to particle, such as fruit granule (such as frustule) can be sent out fluorescence containing pigment, these scattered lights and
Fluorescence is converted into electric signal after being collected by detector and stored, and is automatically analyzed using software.Phytoplankton in water
Cell is used as particle one by one, it is of course possible to carry out flow cytometry, thus can monitor the content of blue-green algae in water.
Publication No. discloses one kind for CN102128779A and CN102128776A Chinese invention patent application and adopted
With Flow cytometry algae and the method for chromophoric dissolved organic matter, General Matters are as follows：Water sample is gathered using injector,
Through filter cleaner, then algae to be detected and organic matter are detected using flow cytometry, and the parameter of detection is led to
Certain algorithm is crossed to try to achieve the number in algae or organic matter cell size and unit volume.Publication No. is
CN102128779A Chinese invention patent application carry out Flow cytometry when, disclose algae to be detected and it is coloured can
Soluble organism is wrapped in by the non-external sheath fluid without any particle flows through flow chamber, is expected that by the fluid of non-external sheath fluid
Mechanics focussing force makes the algae to be detected and the organic matter single-row layout under the coating of sheath fluid, passes sequentially through algae detection
Region, during this, intergranular distance is opened, and it is unicellular or one that detection is only radiated at an algae with laser every time
On organic matter cell, to reach the effect for improving detection accuracy.But, due to not disperseed to the liquid sample of collection
Pretreatment, is only extremely difficult to the effect that individual cells separate and pass sequentially through laser detection area by way of non-external sheath fluid parcel
Really, in addition, above-mentioned two application for a patent for invention does not disclose the concrete mode of sampling, do not disclose the sample mode yet and fit
Waters scope, it detects that algae especially algae big to aggregate density not necessarily has universality, meanwhile, above-mentioned sampling side
Formula is possible to kill alga cells in operation, can cause the further decline of testing result accuracy.
In addition, publication No. discloses a kind of flow cytometry inspection in detail for CN106053302A Chinese invention patent application
Survey device, but the pre-treating method and specific sampling process of collecting sample are not disclosed.
The conventional algae detection method in the country is to gather algae sample using manual site, and laboratory utilization is taken back after fixing
Microscope imaging technology is observed.Micro imaging method includes optical microscope, electron microscopic observation, fluorescence microscopy method etc..
Optical microscope is distinguishable to kind, but pre-treatment work is complicated, and sample identification must be entered by veteran taxology expert
OK, time and effort consuming；Electronic Speculum multiplication factor is high, is conducive to distinguishing doubtful kind, but sample preparation and operation qualification process are excessively complicated；
Fluorescence microscopy observes the biology for being then used for autofluorescence.At present, the domestic algae monitoring carried out also is in the conventional way
It is main, because taxonomic identification workload is larger, it is difficult to adapt to the requirement that field batch samples are quick and precisely analyzed, and can not carry out
Real-time monitoring in situ, in actual applications with certain limitation.
The content of the invention
The present invention provides a kind of algae on-line detecting system for the defect of prior art.
The purpose of the present invention is achieved through the following technical solutions：
A kind of algae on-line detecting system, including water inlet pipe, the bifurcated at one end that the water inlet pipe stretches into sample water source are formed
Multiple water inlet pipes, multiple water inlet pipes are respectively protruding at the different depth at the sample water source, the water inlet pipe it is another
One end is connected with pond, is connected with peristaltic pump and the silt filtering for sampling on the water inlet pipe being connected with the pond in turn
Device；The bottom in the pond is provided with magnetic stirring apparatus, and the side in the pond is provided with ultrasonic cell disintegration instrument, the ultrasound
Cell crushing instrument is connected by cable with one end of ultrasonic transformer, and the other end of the ultrasonic transformer is stretched into the pond, the water
Level sensor is installed, the pond is connected by water pipe with the flow cytometer detected for algae, the water in pond
The sidewall bottom in pond is connected with drainpipe, and the side wall upper part in the pond is connected with overflow pipe；The magnetic stirring apparatus, ultrasound
Cell crushing instrument, flow cytometer, level sensor, magnetic valve, peristaltic pump are electrically connected with electronic control box respectively, the electronics
Control cabinet is electrically connected with terminal computer.
Further, multiple water inlet pipes are connected by magnetic valve with the water inlet pipe respectively.
Further, the water inlet pipe stretches into first water inlet pipe of bifurcated at one end formation at sample water source, the second water inlet branch
Pipe and the 3rd water inlet pipe, the magnetic valve include the first magnetic valve, the second magnetic valve, the 3rd magnetic valve and the 4th magnetic valve,
First water inlet pipe, the second water inlet pipe and the 3rd water inlet pipe pass through the first magnetic valve, the second magnetic valve, the 3rd respectively
Magnetic valve is connected with water inlet pipe, and the water inlet pipe is provided with the 4th magnetic valve and described positioned at one section between peristaltic pump and pond
Silt filter.
Further, first water inlet pipe is located at sample water source bottom 0.5m, and second water inlet pipe is located at sample
This water source bottom 1m, the 3rd water inlet pipe is located at sample water source bottom 1.5m.
Further, in addition to sound proof box, the ultrasonic transformer, magnetic stirring apparatus and pond are located in the sound proof box.
Further, the flow cytometer is CytoSense flow cytometers.
Further, the side wall upper part in the pond is also associated with hot water injection's pipe, is also associated with the water inlet pipe cold
Water flush pipe, is connected with communicating pipe between hot water injection pipe and the cold water flush pipe, hot water injection's pipe, described cold
Water flush pipe is connected to the 5th magnetic valve and the 6th magnetic valve at the pipe end positioned at the communicating pipe side, described
Hot water injection's pipe, the cold water flush pipe have been also respectively connected with control piper switch on the pipeline positioned at the communicating pipe side
The 7th magnetic valve and the 8th magnetic valve.
Further, the bottom of the flow cytometer is provided with exhausted bath box.
Compared with prior art, the present invention at least has advantages below：
1. algae on-line detecting system of the present invention replaces simple self priming pump in sampling process using peristaltic pump, to the greatest extent
The injury in sampling process to algae is possibly reduced, monitoring result is more accurate；And traditional algae monitoring technology then needs to utilize
Fixative fixes algae, and frustule has been damaged, and jolting during transport and preservation can also make to alga cells
Into influence；
2nd, the present invention innovatively adds water sample pre-treatment, is broken up the big group of algae for groupuscule very by physical method
To monomer, be conducive to improving the count accuracy of flow cytometer, sampled water be stirred by magnetic stirring apparatus, it is to avoid
Algae, which floats, influences the accuracy of detection, meanwhile, magnetic agitation is destroyed with scattered will not be produced to algae of ultrasonic vibration；
3rd, the present invention can gather the algae sample at water source vertical direction different water depth, to obtain in water body vertical direction
The feature and changing rule of amount of algae, population structure at diverse location；
4th, the water sample of different algae density is detected that the system is working properly in present invention selection Taihu Lake, shows the algae
On-line monitoring system is suitable for the water body of high concentration algae；The flow cytometer algae on-line monitoring system is applied to national model
The algae on-line monitoring of freshwater lake/reservoir in enclosing, it is applied widely；
5th, flow cytometer algae on-line detecting system of the invention is combined with tele-control system, facilitates policymaker's root
Judge according to the detection for needing to make number and population structure of the algae of monitored area etc. real-time in-situ, and to algae change
Trend is made a prediction, and can either be made detection decision by terminal computer and can be set relevant parameter, realize algae detection
Automation mechanized operation, it is convenient and swift；
6th, flow cytometer algae on-line detecting system of the invention is more efficient compared with traditional algae monitoring technology,
This means algae monitoring system can be gathered and analyze and process more algae samples；
7th, flow cytometer algae on-line detecting system of the invention uses the pattern of full instrumentation, with traditional algae
Monitoring technology needs the situation of artificial micro- sem observation alga cells number and population structure to compare, testing result by it is subjective because
The probability of element interference is relatively low, and accuracy and reliability are higher；
8th, the present invention compared with the flow cytometer of other models, CytoSense flow cytometers it is simple to operate, be suitable to
The algae scope of detection is wide, while the device of the present invention applies also for the detection of organic matter etc., it is applied widely.
Brief description of the drawings
Fig. 1 is the schematic diagram of algae on-line detecting system of the present invention；
Fig. 2 is the signal connection diagram of algae on-line detecting system of the present invention；
Wherein, 1- algae on-line detecting system；2- water inlet pipes；The water inlet pipes of 3- first；The water inlet pipes of 4- second；5- the 3rd
Water inlet pipe；The magnetic valves of 6- first；The magnetic valves of 7- second；The magnetic valves of 8- the 3rd；9- peristaltic pumps；The magnetic valves of 10- the 4th；11- water
Pond；12- magnetic stirring apparatus；13- ultrasonic cell disintegration instrument；14- cables；15- ultrasonic transformers；16- sample feeding pipes；17-CytoSense flows
Formula cell instrument；18- overflow pipes；19- drainpipes；20- columns；21- stations；22- sound proof boxs；23- silt filters；24- cold water
Flush pipe；25- hot water injections manage；The magnetic valves of 26- the 5th；The magnetic valves of 27- the 6th；The magnetic valves of 28- the 7th；The magnetic valves of 29- the 8th；
30- communicating pipes；31- exhausted bath box；32-CytoUSB inspection softwares；33- on-line checking softwares；34- electronic control box；35- terminals
Computer；36-Cytoclus mapping softwares.
Embodiment
The invention will be further described with reference to the accompanying drawings and examples, and following examples are descriptive, are not
Limited, it is impossible to protection scope of the present invention is limited with this.
As shown in figure 1, a kind of algae on-line detecting system 1, including water inlet pipe 2, the water inlet pipe 2 stretches into sample water source
Bifurcated at one end forms multiple water inlet pipes, and multiple water inlet pipes are respectively protruding at the different depth at the sample water source, with
The separately or concurrently water sample of sample drawn water source different depth；Multiple water inlet pipes pass through magnetic valve and the water inlet respectively
Pipe 2 is connected；The other end of the water inlet pipe 2 is connected with pond 11, is connected with turn on the water inlet pipe 2 being connected with the pond 11
For the peristaltic pump 9 and silt filter 23 of sampling, the peristaltic pump 9 is used to draw collection water sample, prevents in destruction water sample
Algae, the silt filter 23 is used to filter the silt in collection water sample；The bottom in the pond 11 is provided with for magnetic force
The magnetic stirring apparatus 12 of the collection water sample in the pond is stirred, the side in the pond 11 is provided with ultrasonic cell disintegration instrument
13, the ultrasonic cell disintegration instrument 13 is connected by cable 14 with one end of ultrasonic transformer 15, and the other end of the ultrasonic transformer 15 is stretched
Enter in the pond 11, the ultrasonic transformer is disperseed by ultrasonic vibration to the collection algae in the pond 11, the water
Pond 11 is built with level sensor, and when water level reaches requirement, water level signal is transferred to Electronic Control by the level sensor
Equipment (electronic control box 34 i.e. hereafter), controls corresponding magnetic valve and peristaltic pump to close, stops to institute by the electronic equipment
State pumping water sample in pond；The pond 11 passes through sample feeding pipe 16 and the CytoSense flow cytometers 17 for algae detection
It is connected, the sidewall bottom in the pond 11 is connected with drainpipe, and the side wall upper part in the pond 11 is connected with overflow pipe 18, institute
State drainpipe be used for detect finish the water sample discharged in the pond 11, the overflow pipe 18 is for by overflow in the pond 11
Water sample drain, the sample feeding pipe 16 be used for convey water sample into the CytoSense flow cytometers 17；The magnetic agitation
Device 12, ultrasonic cell disintegration instrument 13, CytoSense flow cytometers 17, level sensor, magnetic valve, peristaltic pump respectively with electricity
Sub- control cabinet 34 is electrically connected, and the electronic control box 34 is electrically connected with terminal computer 35；
When it is implemented, the water inlet pipe 2 stretches into first water inlet pipe 3 of bifurcated at one end formation at sample water source, second entered
The water inlet pipe 5 of water branch pipe 4 and the 3rd, the magnetic valve includes the first magnetic valve 6, the second magnetic valve 7, the 3rd magnetic valve 8 and the
Four magnetic valves 10, first water inlet pipe 3, the second water inlet pipe 4 and the 3rd water inlet pipe 5 respectively by the first magnetic valve 6,
Second magnetic valve 7, the 3rd magnetic valve 8 are connected with water inlet pipe 2, and the water inlet pipe 2 is located at one section between peristaltic pump 9 and pond 11
It is provided with the 4th magnetic valve 10 and the silt filter 23.
When it is implemented, first water inlet pipe 3 is located at the about 0.5m of sample water source bottom, second water inlet pipe
4 are located at the about 1m of sample water source bottom, and the 3rd water inlet pipe 5 is located at sample water source bottom 1.5m.
When it is implemented, also include sound proof box 22, the ultrasonic transformer 15, magnetic stirring apparatus 12 and pond 11 be located at it is described every
In audio amplifier 22.
When it is implemented, the flow cytometer is CytoSense flow cytometers 17.
When it is implemented, the side wall upper part in the pond 11 is also associated with also connecting on hot water injection's pipe 25, the water inlet pipe 2
Cold water flush pipe 24 is connected to, communicating pipe 30, the heat are connected between hot water injection's pipe 25 and the cold water flush pipe 24
Water flush pipe 25, the cold water flush pipe 24 are connected to the 5th electricity at the pipe end positioned at the side of communicating pipe 30
The magnetic valve 27 of magnet valve 26 and the 6th, hot water injection's pipe 25, the cold water flush pipe 24 are positioned at the side of communicating pipe 30
Pipeline on be also respectively connected with control piper switch the 7th magnetic valve 28 and the 8th magnetic valve 29.
When it is implemented, the bottom of the flow cytometer is provided with exhausted bath box 31.
Embodiment 1
As shown in figure 1, a kind of algae on-line detecting system 1, the algae on-line detecting system 1 is located at the station at sample water source
In room 21, the station 21 is supported on sample water source by column 20, and the algae on-line detecting system 1 is stretched including one end
Enter the water inlet pipe 2 gathered in water sample, the water inlet pipe 2 stretches into bifurcated at one end first water inlet pipe 3, second of formation at sample water source
The water inlet pipe 5 of water inlet pipe 4 and the 3rd, first water inlet pipe 3 is located at the about 0.5m of sample water source bottom, and described second enters
Water branch pipe 4 is located at sample water source bottom 1m, and the 3rd water inlet pipe 5 is located at the about 1.5m of sample water source bottom；Also include the
One magnetic valve 6, the second magnetic valve 7, the 3rd magnetic valve 8, first water inlet pipe 3, the second water inlet pipe 4 and the 3rd water inlet branch
Pipe 5 is connected by the first magnetic valve 6, the second magnetic valve 7, the 3rd magnetic valve 8 with the water inlet pipe 2 respectively；The water inlet pipe 2
The other end is connected with pond 11, is connected with peristaltic pump 9 for sampling, on the water inlet pipe 2 being connected with the pond 11 in turn
Four magnetic valves 10 and silt filter 23；The first magnetic valve 6 and the 4th electricity that selectively unlocking is connected with the first water inlet pipe 3
Magnet valve 10, to realize the detection to sample water source vertical direction bottom about 0.5m depth algae.
The bottom in the pond 11 is provided with magnetic stirring apparatus 12, and the side of pond 11 is provided with ultrasonic cell disintegration instrument
13, the ultrasonic cell disintegration instrument 13 is connected by cable 14 with one end of ultrasonic transformer 15, and the other end of the ultrasonic transformer 15 is stretched
Enter in the pond 11, the pond 11 is connected by water pipe with the CytoSense flow cytometers 17 detected for algae,
The sidewall bottom in the pond 11 is connected with the drainpipe 19 of draining, and the side wall upper part in the pond 11 is connected with for overflow
Overflow pipe 18；
The magnetic stirring apparatus 12, ultrasonic cell disintegration instrument 13, CytoSense flow cytometers 17, level sensor,
One magnetic valve 6, the second magnetic valve 7, the 3rd magnetic valve 8, the 4th magnetic valve 10, peristaltic pump 9, silt filter 23 respectively with electronics
Control cabinet 34 is electrically connected, and the electronic control box 34 is electrically connected with terminal computer 35.
When it is implemented, also include sound proof box 22, the ultrasonic transformer 15, magnetic stirring apparatus 12 and pond 11 be located at it is described every
In audio amplifier 22, to play the effect of sound insulation shock insulation.
A kind of flow cytometer online test method, comprises the following steps：
1) sample, the first magnetic valve 6 and the 4th magnetic valve 10 being connected with the first water inlet pipe 3 are wriggled by described
Water source sample in the collection sampling of pump 9 water source, acquisition rate is 1L/min, and the sample water source is filtered by silt filter 23
Silt.
2) pre-treatment, when the volume of the water source sample reaches 1.5L, stops sampling, starts pretreatment, set the magnetic
The rotating speed of power agitator 12 is 1500r/min；The power for controlling the ultrasonic cell disintegration instrument 13 is 480W, the ultrasonic cell
Broken instrument 13 handles 23 circulations, and each circulation includes ultrasonic vibration 3s, stops 5s.
The water source sample that the magnetic stirring apparatus 12 is pointed in pond 11 is stirred, it is ensured that sample water source it is uniform
Property, avoid algae in water sample from floating the accuracy of the detection of influence CytoSense flow cytometers 17；The ultrasonic cell disintegration instrument
13 carry out ultrasonic vibration by coupled ultrasonic transformer 15 to the water source sample in pond 11, reach fully dispersed algae especially
It is the purpose of common Microcystis aeruginosa (connected and finally flocked together into bulk by ICC between alga cells) in the body of lake,
The big group of algae is broken up as groupuscule even monomer as far as possible.
3) detect, when the pretreatment process is completed, the CytoSense flow cytometers 17 are set using pump speed as 1 μ
L/s extracts the sample water source by pretreatment out of described pond, and collecting sample water source amount is 0.1-0.5ml, while sampling one
Frontier inspection is surveyed, and the sheath fluid chooses pure water, and number, alga cells length, size, the shape of algae are completed to the sample water source of extraction
The parameter detectings such as state, granularity, pigment, peak number, algae colony's feature simultaneously draw corresponding pulse collection of illustrative plates.
The magnetic stirring apparatus 12, ultrasonic cell disintegration instrument 13, CytoSense flow cytometers 17, level sensor,
One magnetic valve 6, the second magnetic valve 7, the 3rd magnetic valve 8, the 4th magnetic valve 10, silt filter 23, peristaltic pump 9 respectively with control
The magnetic stirring apparatus 12, ultrasonic cell disintegration instrument 13, CytoSense flow cytometers 17, level sensor, the first magnetic valve
6th, the Electronic Control that the second magnetic valve 7, the 3rd magnetic valve 8, the 4th magnetic valve 10, silt filter 23, peristaltic pump 9 are operated automatically
Case 34 is electrically connected, and the electronic control box 34 is electrically connected with terminal computer 35, wherein, the level sensor and the electronics control
Two-way electrical connection between case 34 processed, other are unidirectional electrical connection.
As shown in Fig. 2 the control software includes on-line checking software 33, the CytoSense streams that applicant voluntarily researches and develops
CytoUSB inspection softwares 32 and mapping software Cytoclus36 that formula cell instrument 17 is carried, the CytoUSB inspection softwares 32 lead to
Cross terminal computer 35 and CytoSense flow cytometers 17 be two-way electrically connects, the CytoSense flow cytometers 17 with it is described
Electronic control box 34 is unidirectionally electrically connected；The inside of terminal computer 35 is provided with mapping software Cytoclus36, and the drawing is soft
The data that part Cytoclus36 is used to detect the CytoSense flow cytometers 17 are depicted as corresponding chart；It is described
Line inspection software 33 is electrically connected by two-way between terminal computer 35 and the electronic control box 34, the on-line checking software 33
For setting the magnetic stirring apparatus 12, the parameter of ultrasonic cell disintegration instrument 13, the CytoUSB inspection softwares 32 to be used to set
The parameter of CytoSense flow cytometers 17, after relevant parameter is provided with, clicks on operation above-mentioned two software, the end
End computer 35 sends signal to the electronic control box 34, and the electronic control box 34 controls the magnetic stirring apparatus 12, ultrasound
The automatic runnings such as cell crushing instrument 13, CytoSense flow cytometers 17, peristaltic pump 9, magnetic valve.
The annexation and function of the control software of the electronic control box 34 and terminal computer 35 are as follows：
1) the on-line checking software 33 of the electronic control box 34 and the terminal computer 35 transmits mutually data, the electricity
The device data of reception is transferred to on-line checking software 33 by sub- control cabinet 34, and performs the data processing of on-line checking software 33
As a result, the execution is exactly that the data processed result come according to on-line checking software transmission is coupled orderly to run
Equipment.For example, the electronic control box 34 receives the data that level sensor transmits and is transferred to on-line checking software 33, and
The operation of magnetic stirring apparatus 12, ultrasonic cell disintegration instrument 13 is opened according to the result of the feedback of on-line checking software 33.In addition, institute
State electronic control box 34 also had according to the instruction of receiving the first magnetic valve 6 of control, the second magnetic valve 7, the 3rd magnetic valve 8,
The coupled equipment operation such as CytoSense flow cytometers 17, peristaltic pump 9, the 4th magnetic valve 10 or the function of closing, institute
State the electronic control box 34 and control correlation successively according to the step in the online test method of above-mentioned algae on-line detecting system
The operation or closing of equipment.
2) the on-line checking software 33 sets sampling, the operation program of pretreatment in advance, and according in the course of work
The data received from electronic control box 34 to the feedback information of electronic control box 34, the electronic control box 34 according to feedback information come
The operation of the coupled relevant device of control.When sampling, preprocessing process are completed, the on-line checking software 33 is to electronics
Control cabinet 34 sends instruction, and electric signal is sent from electronic control box 34 to CytoSense flow cytometers 17, described
CytoSense flow cytometers 17 transmit the signal for running and starting, the CytoUSB softwares 32 to the CytoUSB softwares 32
Sent to the CytoSense flow cytometers 17 and instruct and control the CytoSense flow cytometers 17 to start according to institute
The detection parameter automatic running that CytoUSB softwares 32 are previously set is stated, to reach the online inspection of above-mentioned algae on-line detecting system
The function of detection in survey method.
3) destination file that the CytoUSB softwares 32 are used to detect the CytoSense flow cytometers 17 is preserved
In the file that terminal computer 35 is specified, the Cytoclus mapping softwares 36 are opened manually, and paint by the Cytoclus
Figure software 36 transfers the destination file that the CytoSense flow cytometers 17 are detected, and relevant parameter detection data are drawn
Shown into corresponding diagrammatic form.
The side wall upper part in the pond is also associated with being also associated with cold water flush pipe on hot water injection's pipe 25, the water inlet pipe
24, it is connected with communicating pipe 30 between hot water injection's pipe 25 and the cold water flush pipe 24, it is hot water injection's pipe 25, described
Cold water flush pipe 24 is connected to the 5th magnetic valve 26 and the 6th electricity at the pipe end positioned at the side of communicating pipe 30
Magnet valve 27, hot water injection's pipe 25, the cold water flush pipe 24 are also distinguished on the pipeline positioned at the side of communicating pipe 30
It is connected with the 7th magnetic valve 28 and the 8th magnetic valve 29 of control piper switch.When in the water sample algae detection complete with
Afterwards, the 5th magnetic valve 26, the 7th magnetic valve 28, the 8th magnetic valve 29, the first magnetic valve 6, the second magnetic valve 7, are first turned on
Three magnetic valves 8 and the 4th magnetic valve 10, are intake water inlet pipe, pond, silt filter, the first water inlet pipe 3, second with cold water
The alga cells of residual in the water inlet pipe 5 of branch pipe 4 and the 3rd are poured in sampling water source, after complete with cold water flush, are closed
5th magnetic valve 26, opens the 6th magnetic valve 27, with hot water by water inlet pipe, pond, by water inlet pipe, pond, silt filter, the
The alga cells of one water inlet pipe, the second water inlet pipe and the residual in the 3rd water inlet pipe are poured in sampling water source, so cold
Hot alternate cycles several times after, developing result is preferable.In addition, in order to obtain more preferable developing result, also may be used during flushing line
To selectively turn on one in the 7th magnetic valve 28, the 8th magnetic valve 29, selective flushing is carried out to pipeline.
The bottom of the flow cytometer is provided with exhausted bath box 31.Detection waste liquid automatic can be discharged into useless by the flow cytometer
In liquid case 31.
Embodiment 2
As shown in figure 1, the first magnetic valve 6 being connected with the first water inlet pipe 3 opened in embodiment 1 is closed, open
The second magnetic valve 7 being connected with the second water inlet pipe 4, realizes the inspection to sample water source vertical direction bottom about 1m depth algae
Survey.
Parameter in a kind of flow cytometer online test method in embodiment 1 is made into modification, it is specific as follows：
1) sample, selectively turn on the second magnetic valve 7 and the 4th magnetic valve 10 being connected with the second water inlet pipe 4,
Water source sample in sampling water source is gathered by the peristaltic pump 9, acquisition rate is 2L/min.
2) pre-treatment, when the volume of the water source sample reaches 3L, starts pretreatment, sets the magnetic stirring apparatus 12
Rotating speed is 3000r/min；The power for controlling the ultrasonic cell disintegration instrument 13 is 300W, and the ultrasonic cell disintegration instrument 13 is handled
33 circulations, each circulation includes ultrasonic vibration 10s, stops 15s.
The water source sample that the magnetic stirring apparatus 12 is pointed in pond 11 is stirred, to ensure the uniform of sample water source
Property, avoid algae from floating accuracy that the follow-up CytoSense flow cytometers 17 of influence detect；The ultrasonic cell disintegration instrument 13
Ultrasonic vibration is carried out to the water source sample in pond 11 by coupled ultrasonic transformer 15, fully dispersed algae is reached especially
The purpose of common Microcystis aeruginosa (connected and finally flocked together into bulk by ICC between alga cells) in the body of lake, to the greatest extent
The big group of algae may be broken up as groupuscule even monomer.
3) detect, when the pretreatment process is completed, the CytoSense flow cytometers 17 are set using pump speed as 2 μ
L/s extracts the sample water source by pretreatment out of described pond 11, and collecting sample water source amount is 0.1-0.5ml, while sampling
While detection, the sheath fluid of CytoSense flow cytometers 17 chooses pure water, and algae is completed to the sample water source of extraction
The parameter detectings such as number, alga cells length, size, form, granularity, pigment, peak number, the special feature of algae group simultaneously draw corresponding
Pulse collection of illustrative plates.
Embodiment 3
As shown in figure 1, the first magnetic valve 6 being connected with the first water inlet pipe 3 opened in embodiment 1 is closed, open
The 3rd magnetic valve 8 being connected with the 3rd water inlet pipe 5, is realized to sample water source vertical direction bottom about 1.5m depth algae
Detection.
Parameter in a kind of flow cytometer online test method in embodiment 1 is made into modification, it is specific as follows：
1) sample, selectively turn on the 3rd magnetic valve 8 and the 4th magnetic valve 10 being connected with the 3rd water inlet pipe 5,
Water source sample in sampling water source is gathered by the peristaltic pump 9, acquisition rate is 0.5L/min.
2) pre-treatment, when the water source sample reaches 1L, starts pretreatment, sets the rotating speed of the magnetic stirring apparatus 12
1000r/min；The power for controlling the ultrasonic cell disintegration instrument 13 is 660W, and the ultrasonic cell disintegration instrument 13 handles 10 and followed
Ring, each circulation includes ultrasonic vibration 7s, stops 10s.
The water source sample that the magnetic stirring apparatus 12 is pointed in pond 11 is stirred, to ensure the uniform of water source sample
Property, avoid algae from floating accuracy that the follow-up CytoSense flow cytometers 17 of influence detect；The ultrasonic cell disintegration instrument 13
Ultrasonic vibration is carried out to the water source sample in pond 11 by coupled ultrasonic transformer 15, fully dispersed algae is reached especially
The purpose of common Microcystis aeruginosa (connected and finally flocked together into bulk by ICC between alga cells) in the body of lake, to the greatest extent
The big group of algae may be broken up as groupuscule even monomer.
3) detect, when the pretreatment process is completed, the setting CytoSense flow cytometers 17 using pump speed as
0.5 μ l/s extract the sample water source by pretreatment out of described pond 11, and collecting sample water source amount is 0.1-0.5ml, on one side
Sampling is while detection, the sheath fluid of CytoSense flow cytometers 17 chooses pure water, and completes algae to the sample water source of extraction
The parameter detectings such as number, alga cells length, size, form, granularity, pigment, peak number, the special feature of algae group of class simultaneously draw phase
The pulse collection of illustrative plates answered.
More than, it is only the present invention preferably embodiment, but the protection domain of invention is not limited thereto, and it is any ripe
Know those skilled in the art the invention discloses technical scope in, the change or replacement that can be readily occurred in should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be defined by the protection domain of claims.
Claims (8)
1. a kind of algae on-line detecting system, including water inlet pipe, the bifurcated at one end that the water inlet pipe stretches into sample water source form many
Individual water inlet pipe, multiple water inlet pipes are respectively protruding at the different depth at the sample water source, the water inlet pipe it is another
End is connected with pond, is connected with peristaltic pump and silt filter for sampling on the water inlet pipe being connected with the pond in turn；
The bottom in the pond is provided with magnetic stirring apparatus, and the side in the pond is provided with ultrasonic cell disintegration instrument, and the ultrasound is thin
Born of the same parents crush instrument and are connected by cable with one end of ultrasonic transformer, and the other end of the ultrasonic transformer is stretched into the pond, the pond
Level sensor is inside installed, the pond is connected by water pipe with the flow cytometer detected for algae, the pond
Sidewall bottom be connected with drainpipe, and the side wall upper part in the pond is connected with overflow pipe；The magnetic stirring apparatus, ultrasound are thin
Born of the same parents crush instrument, flow cytometer, level sensor, magnetic valve, peristaltic pump and electrically connected respectively with electronic control box, the electronics control
Case processed is electrically connected with terminal computer.
2. algae on-line detecting system according to claim 1, it is characterised in that multiple water inlet pipes pass through respectively
Magnetic valve is connected with the water inlet pipe.
3. algae on-line detecting system according to claim 2, it is characterised in that the water inlet pipe stretches into sample water source
The first water inlet pipe of bifurcated at one end formation, the second water inlet pipe and the 3rd water inlet pipe, the magnetic valve include the first magnetic valve,
Second magnetic valve, the 3rd magnetic valve and the 4th magnetic valve, first water inlet pipe, the second water inlet pipe and the 3rd water inlet pipe
It is connected respectively by the first magnetic valve, the second magnetic valve, the 3rd magnetic valve with water inlet pipe, the water inlet pipe is located at peristaltic pump and water
One section between pond is provided with the 4th magnetic valve and the silt filter.
4. algae on-line detecting system according to claim 3, it is characterised in that first water inlet pipe is located at sample
Water source bottom 0.5m, second water inlet pipe is located at sample water source bottom 1m, and the 3rd water inlet pipe is located at sample water source
Bottom 1.5m.
5. algae on-line detecting system according to claim 4, it is characterised in that also including sound proof box, the ultrasonic transformer,
Magnetic stirring apparatus and pond are located in the sound proof box.
6. algae on-line detecting system according to claim 5, it is characterised in that the flow cytometer is
CytoSense flow cytometers.
7. algae on-line detecting system according to claim 1 or 5, it is characterised in that the side wall upper part in the pond is also
It is connected with hot water injection's pipe, the water inlet pipe and is also associated with cold water flush pipe, hot water injection's pipe and the cold water flush
Communicating pipe is connected between pipe, hot water injection's pipe, the cold water flush pipe are in the pipe end positioned at the communicating pipe side
The 5th magnetic valve and the 6th magnetic valve are connected at mouthful, hot water injection's pipe, the cold water flush pipe are positioned at described
Communicating pipe side pipeline on be also respectively connected with control piper switch the 7th magnetic valve and the 8th magnetic valve.
8. algae on-line detecting system according to claim 7, it is characterised in that the bottom of the flow cytometer is provided with
Exhausted bath box.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710513180.7A CN107192657A (en) | 2017-06-29 | 2017-06-29 | A kind of algae on-line detecting system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710513180.7A CN107192657A (en) | 2017-06-29 | 2017-06-29 | A kind of algae on-line detecting system |
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Family
ID=59880498
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| CN201710513180.7A Withdrawn CN107192657A (en) | 2017-06-29 | 2017-06-29 | A kind of algae on-line detecting system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107632550A (en) * | 2017-09-29 | 2018-01-26 | 大连理工大学 | A kind of suspension agitation and filtration concentration acquisition control system and method |
| CN113237817A (en) * | 2021-05-07 | 2021-08-10 | 山东大学 | Depolymerization observation method and application of benthic cyanobacteria algae pad |
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| US5092989A (en) * | 1989-10-19 | 1992-03-03 | Skatron A/S | Sheath fluid filtering in a closed-cycle flow cytometer system |
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Application publication date: 20170922 |