CN107183312A - A kind of method and its application of promotion potato residues drying and dehydrating - Google Patents
A kind of method and its application of promotion potato residues drying and dehydrating Download PDFInfo
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- CN107183312A CN107183312A CN201710413005.0A CN201710413005A CN107183312A CN 107183312 A CN107183312 A CN 107183312A CN 201710413005 A CN201710413005 A CN 201710413005A CN 107183312 A CN107183312 A CN 107183312A
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- pectase
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- drying
- potato residues
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- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 107
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 106
- 238000001035 drying Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000000855 fermentation Methods 0.000 claims abstract description 47
- 230000004151 fermentation Effects 0.000 claims abstract description 46
- 239000002893 slag Substances 0.000 claims abstract description 43
- 241000894006 Bacteria Species 0.000 claims abstract description 38
- 238000012545 processing Methods 0.000 claims abstract description 30
- 108010059892 Cellulase Proteins 0.000 claims abstract description 21
- 229940106157 cellulase Drugs 0.000 claims abstract description 21
- 239000000758 substrate Substances 0.000 claims abstract description 13
- 230000029087 digestion Effects 0.000 claims abstract description 11
- 229940059442 hemicellulase Drugs 0.000 claims abstract description 11
- 108010002430 hemicellulase Proteins 0.000 claims abstract description 11
- 239000007787 solid Substances 0.000 claims abstract description 11
- 241000196324 Embryophyta Species 0.000 claims abstract description 10
- 241000186660 Lactobacillus Species 0.000 claims abstract description 6
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 5
- 108090000790 Enzymes Proteins 0.000 claims description 34
- 102000004190 Enzymes Human genes 0.000 claims description 34
- 229940088598 enzyme Drugs 0.000 claims description 34
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 22
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 22
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 21
- 230000000694 effects Effects 0.000 claims description 20
- 238000011081 inoculation Methods 0.000 claims description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- 230000018044 dehydration Effects 0.000 claims description 9
- 238000006297 dehydration reaction Methods 0.000 claims description 9
- 238000009825 accumulation Methods 0.000 claims description 5
- 230000006835 compression Effects 0.000 claims description 4
- 238000007906 compression Methods 0.000 claims description 4
- 238000007605 air drying Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000001125 extrusion Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 2
- 238000010563 solid-state fermentation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 12
- 241000235342 Saccharomycetes Species 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 239000002028 Biomass Substances 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 239000002054 inoculum Substances 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000002156 mixing Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 235000019750 Crude protein Nutrition 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 3
- 239000008108 microcrystalline cellulose Substances 0.000 description 3
- 229940016286 microcrystalline cellulose Drugs 0.000 description 3
- 239000001814 pectin Substances 0.000 description 3
- 229920001277 pectin Polymers 0.000 description 3
- 235000010987 pectin Nutrition 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 235000010980 cellulose Nutrition 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000001228 trophic effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 108010027322 single cell proteins Proteins 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a kind of method and its application of promotion potato residues drying and dehydrating, belong to biomass resource utilization field.The present invention is using fresh potato slag as fermentation substrate, add at least one of pectase, cellulase, hemicellulase and carry out enzymolysis processing, and inoculated plant lactobacillus and saccharomycete mixed bacteria liquid, solid anaerobic digestion, effectively potato residues can be prevented putrid and deteriorated, improve the nutritive value of potato residues, and cause potato residues fast dewatering, easily dry, with preferable economy and environmental benefit.
Description
Technical field
The present invention relates to a kind of method and its application of promotion potato residues drying and dehydrating, belong to biomass resource utilization neck
Domain.
Background technology
China potato planting area and total output rank the first in the world, have every year substantial amounts of potato be used for starch and
The processing of full powder.During farina is produced, substantial amounts of discarded object potato residues are generated, it is average often to produce 1 ton
Farina about produces 6~7 tons of potato residues.With developing rapidly for farina industry, potato residues can not be obtained
The bottleneck problem of restriction farina processing industry development is had become to good problem of complex utilization.
Potato residues are mainly made up of water, cell fragment and remaining starch particle.The water content of fresh potato slag is reachable
90%, while the also stronger material of the retention ability such as cellulose, hemicellulose and pectin so that potato slag is difficult drying, is dried to
This height, and potato residues carry 33 kinds of bacterium：28 kinds of bacteriums, 4 kinds of moulds, 1 Yeasts, cause potato residues be difficult storage,
Transport, it is easily putrid and deteriorated, produce bad smell.The trans-utilization and synergy problem of potato slag cannot be solved, and easily accumulation is rotten
Lose, cause environmental problem, puzzlement and loss are brought to enterprise.The daily output of potato residues is huge, at present for potato residues
Research remains in the experimental study of small lot, can not still solve anti-corrosion and quick dewatering drying and the heap for causing potato residues
Product corruption, therefore, how processing potato residues rapidly and efficiently, the growth of spoilage organisms in inhibition of potato slag reduces potato
Slag retention ability, realizes potato residues fast dewatering, the nutritive value of potato residues is improved while reduction drying cost, is become
One urgent problem to be solved.
The content of the invention
The technical problem to be solved in the present invention is to provide varied bacteria growing, lifting potato residues product in a kind of inhibition of potato slag
Matter and the method for realizing quick dewatering drying, effectively can prevent potato residues putrid and deteriorated, improve the nutriture value of potato residues
Value, and cause potato residues fast dewatering, easily dry, with preferable economy and environmental benefit.
First purpose of the present invention is to provide a kind of fermentation process of potato residues, methods described using fresh potato slag as
Fermentation substrate, adds at least one of pectase, cellulase, hemicellulase and carries out enzymolysis processing, and inoculated plant breast
Bacillus and saccharomycete mixed bacteria liquid, solid anaerobic digestion.
In one embodiment of the invention, the fresh potato slag, moisture is 85~95%.
In one embodiment of the invention, the enzymolysis processing is with 1~3 with pectase and cellulase：1~10
Ratio mixed, pectase is added in fresh potato slag with 0.25~13U/g addition.
In one embodiment of the invention, the enzymolysis processing is with 1~3 with pectase and hemicellulase：1~
5 ratio is mixed, and pectase is added in fresh potato slag with 0.25~13U/g addition.
In one embodiment of the invention, the enzymolysis processing is with pectase：Cellulase：Hemicellulase is
1~3:1~10:1~5 ratio is mixed, and pectase is added in fresh potato slag with 0.25~13U/g addition
In one embodiment of the invention, mechanical dehydration and drying are also carried out after the solid anaerobic digestion.
In one embodiment of the invention, the inoculation is with 2~14% (bacterium solution volume mL/ fermentation substrate quality
G) inoculum concentration inoculated plant lactobacillus and the mixed bacteria liquid of saccharomycete, the concentration of the mixed bacteria liquid for (1.0~9.9) ×
109CFU/mL。
In one embodiment of the invention, the mixing ratio of Lactobacillus plantarum and saccharomycete is 1 in the mixed bacteria liquid
~4：1~5
In one embodiment of the invention, methods described carries out mechanical agitation mixing after enzyme liquid or bacterium solution is added,
Or use
Layer paving accumulation after uniform sprinkling enzyme and strain mixed liquor.
In one embodiment of the invention, the fermentation is using hermetic bag, melt pit accumulation sealing or uses closed hair
The devices such as fermentation tank realize anaerobic or anaerobic condition.
In one embodiment of the invention, the fermentation temperature is 25~39 DEG C, and fermentation time is 48~120h.
In one embodiment of the invention, the dewatering type be plate compression, screw extrusion, centrifugal dehydration or its
Combination.
In one embodiment of the invention, the drying mode is forced air drying, pneumatic conveying drying, circulation drying, sudden strain of a muscle
It is evaporated dry or its combination.
In one embodiment of the invention, methods described specifically includes following steps：
(1) using fresh potato slag as fermentation substrate, adjustment moisture is 85~95%,
(2) pectase or pectase are mixed with the mixed enzyme of cellulase, hemicellulase with fermentation substrate, enzyme addition
Measure as 0.25~13U/g, mixed proportion is (pectase) 1-3:(cellulase) 1-10:(hemicellulase) 1-5, mechanical agitation
Or stirring makes its be well mixed rear inoculated plant lactobacillus or Lactobacillus plantarum and high-activity yeast bacterium mixed bacteria liquid, bacterium solution inoculation
Measure as 2~14% that (bacterium solution volume mL/ fermentation substrate quality g), mixed bacteria liquid ratio is (Lactobacillus plantarum) 1-4:(high activity ferment
Female bacterium) 1-5.Mechanical agitation or stir is well mixed it, hermetic bag, melt pit accumulation sealing or sealed fermenting tank, realizes anaerobic
Or anaerobic condition, 48~120h of solid anaerobic digestion is carried out at 25~39 DEG C.
(3) after fermentation ends, the dehydration of potato residues is realized using plate compression, screw extrusion, centrifugation.
(4) after being dehydrated, dried using air blast, pneumatic conveying drying, circulation, expansion drying or its combination dry potato
Slag.
Beneficial effect：(1) present invention carries out anaerobic fermentation by being inoculated with mixed microorganism, with reference to enzymatic treatment potato
Slag, based on pectase, supplemented by cellulose or hemicellulose, makes to provide enzymolysis suitable pH in microorganism growth process, and enzyme
The product of solution provides nutrient source for growth, the two is mutually played synergy, and the base of nutriment is not being added additionally
On plinth, fresh potato slag fermentation level is improved.Strain metabolite enriches the trophic component of potato residues, improves potato residues
As the application value of feed, while strengthening the ability for suppressing putrid and deteriorated, and largely make to be combined by potato residues
Water dissociate, realize the fast dewatering and highly effective drying of potato residues, dry moisture content after 120min and be less than 30%, dry
Moisture content is less than 10% after 240min.Compared to the processing such as active ingredient, ensilage in potato residues is extracted, cost advantage is bright
It is aobvious, it can preferably adapt to the present situation and demand of the comprehensive utilization of China's potato residues.
(2) method of the invention can directly handle potato residues, with other wheat mixtures using fresh potato slag as fermentation substrate
The processing of the fermenting raw materials such as bran, rice bran is compared, and significantly reduces pre-treatment cost and intractability, and make at the method for the present invention
Potato residues yield after reason improves 1.38~1.88 times.
(3) present invention uses enzyme combination strain fermentation, enzyme is acted synergistically with microorganism, improves fermentation level, bacterium
Kind metabolite is beneficial to the trophic component of abundant potato residues and inhibition of potato is putrid and deteriorated, moreover it is possible to fresh Ma Ling is greatly reduced
The viscosity of potato slag, strengthens its water separation capability, reduces its intractability while realizing its rapid draing.
(4) solid anaerobic digestion of the present invention, can significantly improve potato residues as the ferment effect of culture medium, contracting
Short fermentation period (60h is to have higher fermentation level), improves the nutritive value of potato residues, and strain enriches beneficial metabolism production
Thing organic acid, single cell protein etc. add the advantage that potato residues are applied to feed.Organic acid in potato residues after fermentation
Content (especially lactic acid) is obviously improved compared to undressed potato slag, and lactic acid content is increased to by 0.0012mg/g
4.787mg/g, crude protein content amplification significantly enriches potato residues as the nutritional ingredient of feed up to 42%.
Brief description of the drawings
Fig. 1 is that enzyme process combination strain fermentation is handled, non-inoculation fermentation is handled and fresh potato slag without any processing is normal
Temperature is gone mouldy situation after placing five days；A, control；Potato residues after B, microbe inoculation；
Fig. 2 is Different treatments potato residues moisture content and the relation of drying time；A is that pectase addition is
0.25U/g, is inoculated with 4% Lactobacillus plantarum；B is only 4% Lactobacillus plantarum of inoculation；C is the potato residues without any processing；D
For pectase addition 3.75U/g, pectase：The enzyme activity ratio of cellulase is 1:1, it is inoculated with 4% Lactobacillus plantarum；E is pectin
Enzyme addition is 0.25U/g, pectase：Cellulase：The enzyme activity ratio of hemicellulase is 1:2:3, the newborn bar of 4% plant of inoculation
Bacterium；F is that pectase addition is 0.25U/g, and pectase, the enzyme activity ratio of cellulase are 1:4；G is farina；H is micro-
Crystalline cellulose；
Fig. 3 is Different treatments potato residues rate of drying and the relation of moisture content；A is that pectase addition is
0.25U/g, is inoculated with 4% Lactobacillus plantarum；B is only 4% Lactobacillus plantarum of inoculation；C is the potato residues without any processing；D
For pectase addition 3.75U/g, pectase：The enzyme activity ratio of cellulase is 1:1, it is inoculated with 4% Lactobacillus plantarum；E is pectin
Enzyme addition is 0.25U/g, pectase：Cellulase：The enzyme activity ratio of hemicellulase is 1:2:3, the newborn bar of 4% plant of inoculation
Bacterium；F is that pectase addition is 0.25U/g, and pectase, the enzyme activity ratio of cellulase are 1:4；G is farina；H is micro-
Crystalline cellulose.
Embodiment
Lactobacillus plantarum in embodiment is Lactobacillus plantarum CCTCC M 2017138 or Lactobacillus plantarum
CCFM8661 (in the national inventing patent for being disclosed in Publication No. CN102586148A), yeast is commercialized Angel soy sauce ferment
Female (Lu Shi yeast).
Embodiment 1
1U/g pectase is added into fresh potato slag, and it is 5.6 × 10 to be inoculated with bacterial concentration9CFU/mL plant breast
The bacterium solutions of bacillus CCTCC M 2017138, inoculum concentration be 10% (bacterium solution volume mL/ fermentation substrate quality g), be well mixed after, 37
Solid anaerobic digestion 72h at DEG C.Centrifugal dehydration after fermentation ends, is determined, and take out a certain amount of to fermentation system total plate count
Potato residues observe its situation of going mouldy at normal temperatures.Using fresh potato slag as control, in the same terms (solid-state anaerobism at 37 DEG C
Ferment 72h) under cultivate.
Shown in measurement result table 1, addition pectase and Lactobacillus plantarum fermentation process can obviously reduce fresh potato slag body
Total plate count in system, total plate count is by 2.20 × 108It is reduced to 3.46 × 107(at least one order of magnitude).Nonvaccinated fresh Ma Ling
Subregion occurs after room temperature 2 days and goes mouldy for potato slag, the potato residues of inoculated plant lactobacillus ferment after placing 5 days,
Do not occur mildew phenomena (Fig. 1) yet.
The change of system total plate count before and after the fresh potato Slag treatment of table 1.
Illustrate that such a processing mode can suppress the growth of other bacterium in fresh potato slag system, reach corrosion-resistant effect, prolong
Its long storage period, normal temperature is closed can be deposited more than 15 days, alleviate the pressure of the processing of the huge fresh potato slag of the daily output.
Embodiment 2
Following material is added into fresh potato slag respectively：
(1) mixed proportion is 1:The pectase of 4 (enzyme activity ratios) and cellulase mixed enzyme solution,；(2) mixed proportion is 1:2:
Pectase, cellulase and the hemicellulase mixed enzyme solution of 3 (enzyme activity ratios), pectase addition are 0.25U/g；(3) mixing ratio
Example is 1:The pectase and cellulase of 1 (enzyme activity ratio), pectase addition are 3.75U/g；(4) pectase addition is
0.25U/g。
(2) after above-mentioned enzyme liquid is well mixed with fresh potato slag, it is 4.40 × 10 that bacterial concentration is inoculated with respectively9CFU/mL
The bacterium solutions of Lactobacillus plantarum CCFM 8661, inoculum concentration 4% (bacterium solution volume mL/ fermentation substrate quality g), be well mixed after, paving
Flat, hermetic bag seals or used membrane closure.Solid anaerobic digestion 72h at 37 DEG C.After fermentation ends, centrifugal dehydration, using air blast
Drying mode, determines the drying characteristic curve of potato residues after different modes processing, using farina and microcrystalline cellulose as
Control.
As a result as shown in Figure 2 and Figure 3, compared to undressed potato residues, the processing of enzyme combination inoculated plant lactobacillus
Potato residues drying property improves significantly.Under the conditions of same dried (exemplified by 55 DEG C), compared to undressed potato
Slag, the potato residues water content after the processing of enzyme process combination strain is low, 160~240min is dried, after the processing of enzyme process combination strain
The water content of potato residues is less than 10%, and unprocessed and only inoculation strain fermentation potato residues still have after drying 240min
Moisture higher than 30%.Potato residues drying time after processing is short, no longer the gluey form with fresh potato slag, and
It is the state with certain fluidity, is post-processed through dehydration, it is loosely organized, pneumatic conveying drying can be achieved.Enzyme process combination strain fermentation
The drying property of the potato residues of processing is better than only inoculation strain fermentation processing and without the potato residues of processing.
Embodiment 3
It is 1 that mixed proportion is added into fresh potato slag:4 pectase and cellulase, using pectase addition as
0.25U/g, is well mixed, inoculated plant lactobacillus and high-activity yeast bacterium mixed bacteria liquid, inoculum concentration is 6%, Lactobacillus plantarum
(CCFM 8661) bacterial concentration is 3.2 × 109CFU/mL, high-activity yeast bacterium rehydration ratio is 40:1 (water quality：Yeast silty
Amount), high-activity yeast bacterium viable count is 7.9 × 107CFU/mL, two kinds of strain mixed proportions are 1:2 (volume ratios), are well mixed
Afterwards, pave, hermetic bag seals or used membrane closure.Solid anaerobic digestion 48h at 37 DEG C.Sample centrifugal dehydration after fermentation ends
Afterwards, forced air drying at 55 DEG C.Record yield after sample drying time and drying end point moisture content, drying.As a result such as Fig. 2 institutes
Show.
Adding enzyme combination strain fermentation processing fresh potato slag, (wet slag, water content is 92%), to dry aqueous after 240min
Rate drops to 10%.And the processing mode of the present invention can substantially reduce the moisture content of potato residues after centrifugation, press filtration.Dry
Afterwards, potato residues have ester fragrance taste, and as feed, can promote the feeding of animal.Potato residues after present invention processing
Water content is 13.4%, and material yield is 7.20g/100g.
Embodiment 4
To fresh potato slag, (wet slag, water content is that 91%) the middle mixed proportion that adds is 1:3 pectase and cellulase,
Pectase addition is 3.75U/g, is well mixed, and inoculum density is 7.4 × 109CFU/mL Lactobacillus plantarum and high activity ferment
Female bacterium mixed bacteria liquid, mixed proportion is 1:2 (volume ratios), (Lactobacillus plantarum is by Southern Yangtze University's bioengineering for Lactobacillus plantarum
Institute provide) inoculum concentration be 8% (bacterium solution volume mL/ fermentation substrate quality g), be well mixed after, pave, hermetic bag seal or adopt
Use membrane closure.Solid anaerobic digestion 48h at 37 DEG C.By pneumatic conveying drying after sample plate compression after fermentation ends.Dry the 10s times
Afterwards, the moisture content of gained sample is 12.3%, and material yield is 5.29g/100g.
Embodiment 5
Pectase 0.25U/g is added into fresh potato slag, pectase is 1 with cellulase mixed proportion：1, mixing is equal
Even, inoculum density is 7.4 × 109CFU/mL Lactobacillus plantarum (CCTCC M 2017138) and high-activity yeast bacterium Mixed Microbes
Liquid, mixed proportion is 1:1 (volume ratio), Lactobacillus plantarum inoculum concentration is 8% (bacterium solution volume mL/ fermentation substrate quality g), mixing
After uniform, pave, hermetic bag seals or used membrane closure.Solid anaerobic digestion 60h at 37 DEG C.Fermentation ends take a certain amount of hair
Ferment product 4500rpm centrifuges 15min, takes supernatant to determine organic acid content.Take sample after a certain amount of fermentation to be freeze-dried, determine
Its crude protein content.
The quantitative Plasma lactate result of potato residues high performance liquid chromatography is shown after the processing of enzyme process combination strain fermentation, lactic acid
Content increases to 4.787mg/g from the 0.0012mg/g of former potato slag.Crude protein content rises to 13.92% from 9.80% (w/w)
(w/w)。
Reference examples 1
Embodiment be the same as Example 4, difference is, without enzyme or strain, or only inoculation strain is fermented, or
Only addition enzyme is handled.
Unprocessed and only inoculation thalline progress fermentation process potato residues are into typical glue, and viscosity is big, no flowing
Property, it is impossible to realize pneumatic conveying drying.The only potato residues fermentation level reduction of addition ferment treatment, organic acid content is only that enzyme process is combined
The half of strain fermentation processing, bacteriostasis property is greatly reduced, and placement starts obvious mildew phenomena occur for 5 days.
Reference examples 2
Embodiment be the same as Example 3, difference is, when pectase addition is 13U/g, aqueous after sample drying
Rate is less than 10%, and the yield of product is only 3.83g/100g.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (10)
1. a kind of fermentation process of potato residues, it is characterised in that methods described using fresh potato slag as fermentation substrate, with 1~
13U/g addition adds at least one of pectase, cellulase, hemicellulase, and inoculated plant lactobacillus and ferment
Female bacterium, is digested and anaerobic solid-state fermentation simultaneously in 25~39 DEG C.
2. according to the method described in claim 1, it is characterised in that pectase is with cellulase with enzyme activity 1~3：1~10 ratio
Example addition, pectase is added in fresh potato slag with 0.25~13U/g addition.
3. according to the method described in claim 1, it is characterised in that the enzymolysis processing be with pectase and hemicellulase with
Enzyme activity 1~3:1~5 ratio addition, pectase is added in fresh potato slag with 0.25~13U/g addition.
4. according to the method described in claim 1, it is characterised in that the enzymolysis processing is with pectase：Cellulase：Half is fine
The plain enzyme of dimension is 1~3:1~10:1~5 ratio addition, pectase adds fresh potato slag with 0.25~13U/g addition
In.
5. according to the method described in claim 1, it is characterised in that the moisture of the fresh potato slag is 85~95%.
6. according to the method described in claim 1, it is characterised in that the inoculation is inoculated with the form of bacterium solution, the bacterium solution
Concentration be (1.0~9.9) × 109CFU/mL；The thalline quantity ratio of Lactobacillus plantarum and yeast is 1 in bacterium solution：1~5；Bacterium solution
Volume：Fermentation substrate quality is 1~7：50mL/g.
7. according to the method described in claim 1, it is characterised in that mechanical dehydration is also carried out after the solid anaerobic digestion and dry
It is dry.
8. method according to claim 7, it is characterised in that the fermentation is using hermetic bag, melt pit accumulation sealing or close
Close fermentation tank.
9. method according to claim 7, it is characterised in that the dewatering type is plate compression, screw extrusion, centrifugation
Dehydration or its combination；The drying mode is forced air drying, pneumatic conveying drying, circulation drying, expansion drying or its combination.
10. potato residues prepared by any methods described of claim 1~9.
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| CN109430534A (en) * | 2018-11-30 | 2019-03-08 | 江南大学 | A kind of method of pair of potato residues dehydration and drying |
| CN116463201A (en) * | 2023-04-16 | 2023-07-21 | 泰安市亿利德农牧机械有限公司 | Potato residue anti-corrosion treatment system and process |
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