CN107180987A - Couple the negative electrode efficient denitrification type microbiological fuel cell of Anammox technology - Google Patents
Couple the negative electrode efficient denitrification type microbiological fuel cell of Anammox technology Download PDFInfo
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Abstract
Carbon system, negative electrode anaerobic ammonia oxidizing bacteria denitrification system, external circuit and online data acquisition system, anaerobism enclosed sampling system, stable gas pressure system, magnetic agitation system are removed the invention discloses the negative electrode efficient denitrification type microbiological fuel cell of coupling Anammox technology, including anode electrochemical active bacteria.Intermittent duty of the present invention, apparatus structure is simple, compared with conventional cathode denitrification denitrogenation type MFC, significantly, nitrogen load is significantly improved denitrification effect, and electricity production works well, practicality increases, and is that microbiological fuel cell and Anammox technology hew out a kind of new application.
Description
Technical field
The invention belongs to microorganism electrochemical systems technology field, more particularly to a kind of the moon for coupling Anammox technology
Extremely efficient denitrogenation-type microbiological fuel cell.
Background technology
In recent years, with industry size expansion and mankind's activity aggravation, the nitrogen in sanitary sewage and industrial wastewater is dirty
The trend that concentration increases, discharge capacity increases is presented in dye, and the random discharge of high nitrogen-containing waste water not only easily causes water eutrophication
Change, can also largely consume the dissolved oxygen in water, cause serious water body environment to destroy.Simultaneously because the energy day is becoming tight, pollute
Energy consumption problem in governance process is gradually taken seriously, exploitation energy saving technique and the primary study that product is domestic and international environmental protection sector
Direction.
Microbiological fuel cell (Microbial Fuel Cell, abbreviation MFC) is a kind of using electricity-producing microorganism as catalysis
Agent, the chemical energy that be will be stored in by biodegradation in pollutant is converted into the device of electric energy, negative electrode denitrogenation-type MFC
As one kind of its application, simultaneous denitrification is had concurrently and has removed the performance that carbon is produced electricity, with suitable realistic meaning and application prospect.Its
In anode, organic matter is degraded generation electronics, and electronics, which passes through, immediately reaches negative electrode electricity after anode electrode, external circuit, external resistance
The nitrogen pollutants such as pole, nitrate nitrogen and nitrite nitrogen obtain electronics in negative electrode and are reduced into N2.Current denitrogenation-type MFC researchs are main
Nitrogen, but its common defect are removed based on reactions such as synchronous nitration and denitrification, short-cut nitrification and denitrification, synchronous decarburization ammoxidations
It is that reaction scheme length, surplus sludge volume be big, high energy consumption, system complex, maintenance cost is high.
Anammox technology (Anaerobic Ammonium Oxidation, abbreviation Anammox) is one kind while going
Except the formula efficient denitrification technology of ammonia nitrogen and nitrite nitrogen, it is under anaerobic ammonia oxidizing bacteria effect, using ammonium salt as electron donor, nitrous acid
Salt is electron acceptor, and two kinds of pollutants are converted into nitrogen by a step simultaneously.Anammox is coupled with double chamber type MFC, negative electrode is created
It is most emerging theory that voltage output is obtained while efficient denitrification environment, and it is low that this can not only solve MFC bacterium nitrogen loads
Defect, moreover it is possible to give Anammox technologies provide a brand-new application direction, well using its high nitrogen load, low sludge concentration,
The characteristics of high reaction rate, highly further research.
MFC of the present invention principle is：Yin, yang pole room is separated by PEM, forms relatively independent reaction ring
Border, after bioelectrochemistry is coupled successfully, cathode and anode reaction is contacted and influenced each other by electron transmission formation, second in anode
Hydrochlorate is simultaneously enriched on anode electrode by electro-chemical activity bacterium catalytic degradation, the electron transmission of generation, and then it passes through external circuit
Cathode electrode is transferred to, cathode chamber is while occurring Anammox reaction, and the nitrate of generation is entered one because obtaining electronics
Step is removed.
The content of the invention
It is a primary object of the present invention to the defect for overcoming existing negative electrode denitrogenation-type MFC, there is provided one kind coupling anaerobism ammonia oxygen
The negative electrode efficient denitrification type microbiological fuel cell of change technology, concrete technical scheme is as follows.
A kind of negative electrode efficient denitrification type microbiological fuel cell for coupling Anammox technology, including anode electrochemical are lived
Property bacterium remove carbon system, negative electrode anaerobic ammonia oxidizing bacteria denitrification system, external circuit and online data acquisition system, anaerobism enclosed sampling system
System, stable gas pressure system, magnetic agitation system；Wherein, anode electrochemical active bacteria removes the structural relation of each part in carbon system
For：Filled on the left of anode chamber first match somebody with somebody acrylic dividing plate, centre add the first silica gel pad and using screw rivet tightly progress it is close
Piece titanium silk of insertion inside anode electrode carbon felt, carbon felt is internally embedded on the right side of envelope, anode chamber, and is led to outside anode room for outer
Circuit connection, the full electro-chemical activity bacterium of anode compartment filling and anolyte；In negative electrode anaerobic ammonia oxidizing bacteria denitrification system
The structural relation of each part is：Another second acrylic dividing plate is assembled on the right side of cathode chamber, the second silica gel pad is added simultaneously in centre
Riveted tightly and sealed using screw, be internally embedded on the left of cathode chamber on the right side of cathode electrode carbon cloth, carbon cloth and apply the catalysis of last layer platinum carbon
Layer, and another titanium silk is aside brought into close contact, and lead to for external circuit line outside negative electrode room, the filling of cathode chamber inside is full
Anaerobic ammonia oxidizing bacteria and catholyte；The structural relation of each part is in external circuit and online data acquisition system：By anode
Electro-chemical activity bacterium is except carbon system and the progress split of negative electrode anaerobic ammonia oxidizing bacteria denitrification system, and centre sandwiches PEM, matter
The both sides of proton exchange are equipped with silica gel pad, and riveting tightly progress sealing using screw constitutes a coupling entirety, then using copper
Wire processed is connected to two titanium silks, parallel connection access resistance box, then is connected to data acquisition unit progress data acquisition, and the complete period is not
Intermitten service；The structural relation of each part is in the anaerobism enclosed sampling system：On the left of anode chamber, on the right side of cathode chamber respectively
Punch and insert acrylic pipe, use each one medical three-way valve of connection, and access disposable injection behind the gluing jail of acrylic
Device；The structural relation of each part is in the stable gas pressure system：Punched respectively at the top of anode chamber, cathode chamber and insert Asia gram
Solenoid, uses each one medical three-way valve of connection, and access smalt syringe behind the gluing jail of acrylic；Magnetic agitation system
In the structural relation of each part be：Magnetic stir bar is added inside anode chamber, cathode chamber, magnetic stirring apparatus is placed in bottom.
Further, the reactor anode chamber is identical with cathode chamber configuration size, dischargeable capacity about 28mL, sample introduction
Mouth and sample tap are ensured the environment strictly anaerobic of dual chamber in sampling process by medical three-way valve, pass through one in two pole room sides
Secondary property syringe sampling, constitutes anaerobism enclosed sampling system.
Further, the reactor head sets stable gas pressure system, is received using the minimum smalt syringe of resistance
Collect the gas produced, mainly include the issuable CO of anode2The N produced with negative electrode2, ensure to take a breath by medical three-way valve
The strictly anaerobic of dual chamber in journey, constitutes stable gas pressure system.
Further, the reactor yin, yang pole room uses magnetic agitation system, the mixing of its electrolyte is more filled
Point, the magnetic stirring apparatus complete period runs without interruption, and control rotating speed constitutes magnetic agitation system in 200r/min.
Further, the reactor anode uses carbon felt as electrode, and negative electrode uses carbon cloth as electrode, carbon felt and carbon
Cloth is 3.0 ± 0.1cm of diameter circle, and carbon felt thickness is 0.50 ± 0.05cm；Carbon felt and carbon cloth are passing through pre- before
Processing, its pretreatment mode is successively successively to be soaked in 30%H under 50 ± 1 DEG C of waters bath with thermostatic control2O2, ultra-pure water, 0.5mol/L
Each 1 hour of sulfuric acid, ultra-pure water.Then, 10% platinum carbon 0.50 ± 0.02mg of Catalytic Layer Pt/cm are smeared on negative electrode carbon cloth2Promote cloudy
Pole reduction reaction.
Further, the reactor anolyte for institute's carbonaceous sources 0.010 ± 0.001mol/L sodium acetates buffering
Solution, its initial pH is 8.0 ± 0.2,；Catholyte is that contained 400 ± 15mg/L of nitrogen source ammonium sulfate and 528 ± 20mg/L are sub-
The high nitrogen load nutrient solution of sodium nitrate, its initial pH is 7.0 ± 0.5.
Further, every liter of 5.88g containing NaCl of the reactor anolyte cushioning liquid, KCl 0.1g, NH4Cl
0.25g、Na2HPO4·12H2O 33.92g、NaH2PO4·12H2O 0.827g；Negative electrode culture formula of liquid is (every liter contains) KH2PO4
0.1318g、CaCl2 0.18g、MgSO4 0.3g、NaHCO31g, error range is less than ± 5%, without organic carbon source.
Further, the reactor anode inoculation electro-chemical activity bacterium, is derived from sewage treatment plant's anaerobic pond with de-
The active microorganism of nitrogen function, its bacterium colony composition is mainly Geobacter Pseudomonas；Negative electrode is inoculated with anaerobic ammonia oxidizing bacteria, and being derived from makes
With the UASB reactors of Anammox technologies, its bacterium colony composition is mainly Anammoxaceae Pseudomonas.
Further, the reactor complete period is placed in 33 ± 0.5 DEG C of constant incubators, its yin, yang pole room complete period
Keep strictly anaerobic environment.
Further, the reactor uses batch intermittent duty, and sun is changed when cell output voltage is less than 10mV
Pole electrolyte, substitute mode supplements the electro-chemical activity bacterium solution of independent culture to take out the half of original solution, then；Whenever the moon
Pole electrolyte total nitrogen concentration changes catholyte when being less than 30mg/L, substitute mode is then supplemented to take out all original solutions
The catholyte newly prepared.
Further, voltage data of the present invention by Keithley M2700 data acquisition units gather, ammonia nitrogen, nitrite nitrogen,
Nitrate is determined and followed《Water and effluent monitoring analysis method》(fourth edition), bacterial community analysis is obtained by 16S rRNA technologies
.
Compared with the prior art, the present invention has the advantages that：
(1) simultaneous denitrification can be achieved except carbon electricity production without add organic carbon source in negative electrode in the present invention, it is to avoid organic carbon
What source was caused has community evolution and the competition of negative effect.
(2) negative electrode nitrogen load ability can be greatly improved using Anammox technology, it is to avoid common nitrification, denitrification
Bacterium is because of activity suppression caused by influent ammonia nitrogen, nitrite nitrogen excessive concentration.
(3) ammonia nitrogen of the present invention, nitrite nitrogen, nitrogen removal rate and removal rate are greatly improved.
(4) anaerobism enclosed sampling system and air pressure systems stabilisation enhance the air-tightness of reactor, improve the property of reactor
The accuracy of energy and test result；The use of magnetic agitation system enhances the uniformity coefficient of cathode and anode liquid, it is to avoid because dense
Spend the situation appearance that gradients affect reaction is carried out.
(5) compared with traditional Anammox technology, the present invention makes the NO of generation3 -Ion is removed by electrochemical method,
Enhance the clearance of total nitrogen.
(6) pre-processed by electrode, the abundant domestication of bacterium colony, output voltage of the present invention compares other negative electrode denitrogenation-types MFC
It is significantly improved.
Brief description of the drawings
Fig. 1 is a kind of structure for the negative electrode efficient denitrification type microbiological fuel cell for coupling Anammox technology of the present invention
Schematic diagram；
Fig. 2 be using the present invention microbial dye battery respectively 1#, 2#, 3#, 4#-MFC negative electrode apply containing
200mg/L N-NH4 +、264mg/L N-NO2 -；300mg/L N-NH4 +、396mg/L N-NO2 -；400mg/L N-NH4 +、
528mg/L N-NO2 -；500mg/L N-NH4 +、660mg/L N-NO2 -A high nitrogen load waste water corresponding cycle for four kinds of concentration
Produce electricity schematic diagram.
Embodiment
The embodiment to the present invention is explained below in conjunction with the accompanying drawings, but the implementation and protection of the present invention are not limited to
This.
Such as Fig. 1, a kind of negative electrode efficient denitrification type microbiological fuel cell of coupling Anammox technology mainly includes sun
Pole electro-chemical activity bacterium is except carbon system, negative electrode anaerobic ammonia oxidizing bacteria denitrification system, external circuit and online data acquisition system, anaerobism
The big main body of enclosed sampling system, stable gas pressure system, magnetic agitation system etc. six.Carbon system is removed including anode electrochemical active bacteria
System, negative electrode anaerobic ammonia oxidizing bacteria denitrification system, external circuit and online data acquisition system, anaerobism enclosed sampling system, air pressure are steady
Determine system, magnetic agitation system；Wherein, anode electrochemical active bacteria removes the structural relation of each part in carbon system and is：In anode
The left side of room 8 fills first and matches somebody with somebody acrylic dividing plate 3, the first silica gel pad 4 is added in centre and is riveted tightly using screw is sealed, anode
Piece titanium silk 10 of insertion inside anode electrode carbon felt 14, carbon felt is internally embedded on the right side of room, and is led to outside anode room for dispatch from foreign news agency
Road line, the full electro-chemical activity bacterium of anode compartment filling and anolyte 19；In negative electrode anaerobic ammonia oxidizing bacteria denitrification system
The structural relation of each part is：In the another second acrylic dividing plate of the right side of cathode chamber 18 assembling, the second silica gel pad is added in centre
And riveted tightly and sealed using screw, it is internally embedded on the left of cathode chamber on the right side of cathode electrode carbon cloth 16, carbon cloth and applies last layer platinum carbon
Catalytic Layer 17, and be aside brought into close contact another titanium silk, and lead to outside negative electrode room for external circuit line, inside cathode chamber
The full anaerobic ammonia oxidizing bacteria of filling and catholyte 19；The structural relation of each part in external circuit and online data acquisition system
For：By anode electrochemical active bacteria except carbon system and the progress split of negative electrode anaerobic ammonia oxidizing bacteria denitrification system, centre sandwiches proton
Exchange membrane 15, the both sides of PEM 15 are equipped with silica gel pad, and it is whole to rivet tightly progress sealing one coupling of composition using screw
Body, is then connected to two titanium silks, parallel connection access resistance box 11 using copper wire 13, then is connected to data acquisition unit 12
Row data acquisition, the complete period runs without interruption；The structural relation of each part is in the anaerobism enclosed sampling system：In anode chamber
8 left sides, the right side of cathode chamber 18 are punched and insert acrylic pipe 6 respectively, using each connection one is medical behind the gluing jail of acrylic
Triple valve 2, and access disposable syringe 1；The structural relation of each part is in the stable gas pressure system：In anode chamber 8, the moon
The top of pole room 18 is punched and inserts acrylic pipe respectively, using one medical three-way valve of respective connection behind the gluing jail of acrylic, and
Access smalt syringe 5；The structural relation of each part is in magnetic agitation system：Add inside anode chamber 8, cathode chamber 18
Enter magnetic stir bar 7, magnetic stirring apparatus 9 is placed in bottom.
The negative electrode efficient denitrification type microbiological fuel cell of above-mentioned coupling Anammox technology is external in start-up course
The Ω of resistance 2000, yin, yang pole room changes once electrolytic liquid for every 2 days simultaneously, and substitute mode is electric for the old anode for extracting half volume
Liquid is solved, new anolyte is supplemented to full, extracts whole old catholytes, new catholyte is supplemented to full, cloudy,
Anolyte is aerated 10 minutes by nitrogen.
Domestication forms burning voltage output after about 3 weeks, be considered as domestication and complete, and replacing outer meeting resistance is 1000 Ω, now coupling
The negative electrode efficient denitrification type microbiological fuel cell course of work for closing Anammox technology is as follows：(1) whenever battery exports electricity
Force down and anolyte is changed when 10mV, specific steps include closing anode-side magnetic stirring apparatus；Open anode-side anaerobism close
Close sampling system and corresponding 2 medical three-way valves of air pressure systems stabilisation；Using 5mL disposable syringes by the old anode of half
Liquid is extracted out；The anode simulated wastewater then added using new syringe after the nitrogen aeration newly prepared；Solution is full of anode chamber
2 medical three-way valves are closed afterwards；Anode-side 5mL smalt syringes are extracted, are turned back to after discharge internal gas；Magnetic force is opened to stir
Mix device.(2) catholyte is changed when catholyte total nitrogen concentration is less than 30mg/L, specific steps include closing negative electrode
Side magnetic stirring apparatus；Open cathode side anaerobism enclosed sampling system and corresponding 2 medical three-way valves of air pressure systems stabilisation；Use
5mL disposable syringes extract whole old catholytes out；Then added using new syringe after the nitrogen aeration newly prepared
Cathode Numerical Simulation of A waste water；Solution, which is full of after cathode chamber, closes 2 medical three-way valves；Cathode side 5mL smalt syringes are extracted,
Turned back to after discharge internal gas；Open magnetic stirring apparatus.
Anolyte contains NaAc, NaCl, KCl, NH4Cl、Na2HPO4·12H2O、NaH2PO4·12H2O, and it is micro
Element M gSO4、MnSO4、FeSO4、CaCl2、CoCl2、ZnCl2、CuSO4、AlK(SO4)2·12H2O、H3BO3、Na2MoO4、
NiCl2、Na2WO4·2H2O, and biotin, folic acid, vitamin B6, riboflavin B2, thiamine hydrochloride B1, nicotinic acid B3, D-VB5 calcium
B5, cobalamin B12, p-aminobenzoic acid, lipoic acid.
Catholyte contains KH2PO4、CaCl2、MgSO4、NaHCO3, micro- I includes EDTA, FeSO4, and it is micro
Element II includes ZnSO4·7H2O、CuSO4·5H2O、MnCl2·4H2O、Na2MoO4·2H2O、NiCl2·6H2O、CoCl2·
6H2O、H3BO3。
During Fig. 2 is embodiment, using containing 200mg/L N-NH4 +、264mg/L N-NO2 -；300mg/L N-NH4 +、
396mg/L N-NO2 -；400mg/L N-NH4 +、528mg/L N-NO2 -；500mg/L N-NH4 +、660mg/L N-NO2 -Four kinds dense
The high nitrogen load waste water (its concentration error scope is less than 5%) of degree is as 1#, 2#, 3#, 4#-MFC catholyte, after water inlet
The electricity production schematic diagram in a cycle is run, its concentration error scope is less than 5%.
It can be seen that the ceiling voltage that 4 groups of MFC reach in stable operation is respectively 322.90,351.53,360.84 and
247.41mV。
Table 1 is the corresponding ammonia nitrogen of above-mentioned 4 groups of reactors, nitrite nitrogen, total nitrogen go division result to show.
Table 1
Table 1 is in embodiment, above-mentioned corresponding 4 groups of MFC reactors nitrogen removal performance shows, it can be seen that 1#, 2#, 3#-MFC
Ammonia nitrogen, nitrite nitrogen, the clearance of total nitrogen respectively more than 93%, 83% and 77%, wherein 3-MFC ammonia nitrogen removal frank surpass
The nitrogen removal performance for having crossed 98%, 4#-MFC is relatively weak.
When can be seen that the operation MFC of the present invention using different N concentration waste water as catholyte from upper several experimental datas,
Electricity production and denitrification ability are not in very big gap, but if nitrogen concentration is more than a threshold value, such as water inlet 500mg/L N-NH4 +、
660mg/L N-NO2When, its output voltage and denitrification ability are a greater impact.
Final statement, above citing is only that the present invention is still present using one of some actual examples of the invention applied
Many optimizations and deformation, various optimizations and change that one of ordinary skill in the art is associated from present disclosure
Shape, is such as considered as protection scope of the present invention with the present invention without difference substantially.
Claims (10)
1. couple the negative electrode efficient denitrification type microbiological fuel cell of Anammox technology, it is characterised in that including anode electrification
Active bacteria is learned except carbon system, negative electrode anaerobic ammonia oxidizing bacteria denitrification system, external circuit and online data acquisition system, anaerobism are closed adopts
Sample system, stable gas pressure system, magnetic agitation system；Wherein, anode electrochemical active bacteria removes the structure of each part in carbon system
Relation is：In anode chamber（8）Left side fills first and matches somebody with somebody acrylic dividing plate（3）, the first silica gel pad is added in centre（4）And use spiral shell
Nail, which is riveted tightly, to be sealed, and anode electrode carbon felt is internally embedded on the right side of anode chamber（14）, piece titanium silk of carbon felt inside insertion（10）, and
Lead to outside anode room for external circuit line, the full electro-chemical activity bacterium of anode compartment filling and anolyte（19）；It is cloudy
The structural relation of each part is in the anaerobic ammonia oxidizing bacteria denitrification system of pole：In cathode chamber（18）Right side assemble another second acrylic every
Plate, is added the second silica gel pad and being riveted tightly using screw in centre and is sealed, cathode electrode carbon is internally embedded on the left of cathode chamber
Cloth（16）, carbon cloth right side applies last layer platinum carbon Catalytic Layer（17）, and another titanium silk is aside brought into close contact, and lead to negative electrode
Outdoor is for external circuit line, the full anaerobic ammonia oxidizing bacteria of cathode chamber inside filling and catholyte（19）；External circuit and data
The structural relation of each part is in on-line acquisition system：Anode electrochemical active bacteria is removed into carbon system and negative electrode anaerobic ammonia oxidizing bacteria
Denitrification system carries out split, and centre sandwiches PEM（15）, PEM（15）Both sides be equipped with silica gel pad, make
Riveted tightly with screw and carry out one coupling entirety of sealing composition, then use copper wire（13）Two titanium silks are connected to, and
Couple into resistance box（11）, then it is connected to data acquisition unit（12）Data acquisition is carried out, the complete period runs without interruption；The anaerobism is close
The structural relation for closing each part in sampling system is：In anode chamber（8）Left side, cathode chamber（18）Punch respectively and insert Asia in right side
Gram force pipe（6）, use one medical three-way valve of respective connection behind the gluing jail of acrylic（2）, and access disposable syringe（1）；
The structural relation of each part is in the stable gas pressure system：In anode chamber（8）, cathode chamber（18）Punch and insert respectively in top
Acrylic pipe, uses each one medical three-way valve of connection, and access smalt syringe behind the gluing jail of acrylic（5）；Magnetic force
The structural relation of each part is in stirring system：In anode chamber（8）, cathode chamber（18）Inside adds magnetic stir bar（7）, bottom
Place magnetic stirring apparatus in portion（9）.
2. the negative electrode efficient denitrification type microbiological fuel cell of coupling Anammox technology according to claim 1, its
It is characterised by that anode chamber is identical with cathode chamber configuration size, the mL of dischargeable capacity about 28, injection port and sample tap are located in two
Pole room side, is ensured the environment strictly anaerobic of dual chamber in sampling process by medical three-way valve, is sampled by disposable syringe.
3. the negative electrode efficient denitrification type microbiological fuel cell of coupling Anammox technology according to claim 1, its
The stable gas pressure system is characterised by, the gas produced using the minimum smalt syringe collecting of resistance is main to include sun
The CO most probably produced2The N produced with negative electrode2, ensure the strictly anaerobic of dual chamber in scavenging period by medical three-way valve.
4. the negative electrode efficient denitrification type microbiological fuel cell of coupling Anammox technology according to claim 1, its
It is characterised by that yin, yang pole room uses magnetic agitation system, mixes its electrolyte more abundant, the magnetic stirring apparatus complete period is not
Intermitten service, it is 200 r/min to control rotating speed.
5. the negative electrode efficient denitrification type microbiological fuel cell of coupling Anammox technology according to claim 1, its
Be characterised by that anode uses carbon felt as electrode, negative electrode uses carbon cloth as electrode, carbon felt and carbon cloth be diameter 3.0 ±
0.1 cm circle, carbon felt thickness is 0.50 ± 0.05 cm；Carbon felt and carbon cloth are passing through pretreatment before, and it is pre-processed
Mode is successively successively to be soaked in 30% under 50.0 ± 0.5 °C of waters bath with thermostatic control（Mass fraction）H2O2, ultra-pure water, 0.5
Each 1 hour of mol/L sulfuric acid, ultra-pure water；Then, 10% is smeared on negative electrode carbon cloth（Mass fraction）Platinum carbon Catalytic Layer 0.50 ±
0.02 mg Pt/cm2。
6. a kind of negative electrode efficient denitrification type Microbial fuel electricity for coupling Anammox technology according to claim 1
Pond, it is characterised in that anolyte is the cushioning liquid of the mol/L sodium acetates of institute's carbonaceous sources 0.010 ± 0.001, and its is initial
PH is 8.0 ± 0.2,；Catholyte is the contained mg/L ammonium sulfate of nitrogen source 400 ± 8 and 528 ± 11 mg/L nitrous acid
The high nitrogen load nutrient solution of sodium, its initial pH is 7.0 ± 0.5.
7. a kind of negative electrode efficient denitrification type Microbial fuel electricity for coupling Anammox technology according to claim 1
Pond, it is characterised in that liquid cushioning liquid formula is every liter containing NaCl 5.88 g, KCl 0.1 g, NH in anode4Cl 0.25 g、
Na2HPO4·12H2O 33.92 g、NaH2PO4·12H2The g of O 0.827,；Formula of liquid is cultivated in negative electrode and contains KH for every liter2PO4
0.1318 g、CaCl2 0.18 g、MgSO4 0.3 g、NaHCO31 g, error range is less than ± 5%, without organic carbon source.
8. a kind of negative electrode efficient denitrification type Microbial fuel electricity for coupling Anammox technology according to claim 1
Pond, it is characterised in that anode is inoculated with electro-chemical activity bacterium, is derived from active micro- with denitrification functions in sewage treatment plant's anaerobic pond
Biology, its bacterium colony composition is mainly Geobacter Pseudomonas；Negative electrode is inoculated with anaerobic ammonia oxidizing bacteria, is derived from using Anammox technologies
UASB reactors, its bacterium colony composition is mainly Anammoxaceae Pseudomonas.
9. a kind of negative electrode efficient denitrification type Microbial fuel electricity for coupling Anammox technology according to claim 1
Pond, it is characterised in that the reactor complete period being made up of anode chamber, cathode chamber is placed in 33 ± 0.5 °C of constant incubators, its
The yin, yang pole room complete period keeps strictly anaerobic environment.
10. a kind of negative electrode efficient denitrification type Microbial fuel electricity for coupling Anammox technology according to claim 1
Pond, it is characterised in that batch intermittent duty, anolyte, substitute mode are changed when cell output voltage is less than 10 mV
To take out the half of original solution, the electro-chemical activity bacterium solution that then supplement is independently cultivated；Whenever catholyte total nitrogen concentration
Catholyte is changed during less than 30 mg/L, substitute mode then supplements the catholyte newly prepared to take out all original solutions
Liquid.
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| CN109264850A (en) * | 2018-11-09 | 2019-01-25 | 河北大学 | Integral anaerobic ammoxidation film bioelectrochemical system and sewage water denitrification remove the treatment process of carbon |
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| CN109638327B (en) * | 2018-12-19 | 2021-05-18 | 大连理工大学 | Process for denitrification and power generation by using single-chamber anaerobic ammonia oxidation sludge-microbial fuel cell device |
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| CN111573834A (en) * | 2020-05-22 | 2020-08-25 | 盐城工学院 | Reactor based on short-cut denitrification electrode is in coordination with anaerobic ammonia oxidation denitrogenation |
| CN111573821A (en) * | 2020-05-22 | 2020-08-25 | 盐城工学院 | Electrode denitrification system based on autotrophic short-cut denitrification-anaerobic ammonia oxidation module |
| CN113336315A (en) * | 2021-07-05 | 2021-09-03 | 华北电力大学（保定） | System and method for treating sewage by using microbial fuel cell |
| CN113336315B (en) * | 2021-07-05 | 2022-04-12 | 华北电力大学（保定） | System and method for treating sewage by using microbial fuel cell |
| CN113800620A (en) * | 2021-09-07 | 2021-12-17 | 华东师范大学 | Denitrification and desulfurization reaction device based on bioelectrochemistry |
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