CN107179410A - A kind of DNA detection means and detection method - Google Patents
A kind of DNA detection means and detection method Download PDFInfo
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- CN107179410A CN107179410A CN201710537690.8A CN201710537690A CN107179410A CN 107179410 A CN107179410 A CN 107179410A CN 201710537690 A CN201710537690 A CN 201710537690A CN 107179410 A CN107179410 A CN 107179410A
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- test tube
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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Abstract
The invention discloses a kind of DNA detection means and detection method, including detection means, include sampling test tube on detection means, nitrocellulose filter rod, filtration manifold, control area, detector, computer, filter mouth, filter block, when carrying out DNA detections, saliva is used in many cases, oral cavity epicuticle or blood carry out DNA detection, the cell tissue detected in the technical scheme using oral cavity epicuticle as DNA, the cell detected using oral cavity epicuticle as DNA can be accomplished painless, without wound, extract convenient and swift, the detection means detection used in the technical scheme, with very high sensitivity and reliability, the DNA of product can fast and accurately be examined and determine.
Description
Technical field
The present invention relates to DNA detection technique fields, specially a kind of DNA detection means and detection method.
Background technology
DNA is a kind of long-chain polymer, and composition unit is four kinds of deoxynucleotides, i.e. adenyl-deoxyribonucleotide (dAMP
Desoxyadenossine), thymidylic acid (dTMP AZTs), deoxycytidylic acid (dCMP deoxycytidines), bird
Purine deoxyriboside acid (dGMP deoxyguanosines).
DNA is a kind of critically important inhereditary material.DNA is also known as nuclifort, is a kind of molecule, can
Genetic command is constituted, to guide biological development to be operated with vital functions.Major function is the information storage of chronicity, can be likened to
" blueprint " or " recipe ".The instruction wherein included, is the intracellular other compounds of construction, as needed for protein and RNA.Carry
The DNA fragmentation of hereditary message is referred to as gene, other DNA sequence dnas, and some are directly played a role with construction itself, and some are then participated in
The performance of the hereditary message of regulation and control.
DNA detections are the detections in nucleic acid level, and common method has PCR, genetic chip etc.；Have to use and be referred to as
PCR (polymerase chain reaction) technology, makes the amount needed for the desired DNA/RNA fragment amplifications to detection in gene, carries out
The method of detection.In addition, as its development form, many quantifying using referred to as qPCR (quantitative PCR, quantitativePCR)
Analytical technology.The quantitative analysis tech of these genes is directed to mini-reactor in microfluidic circuit chip etc..
The present invention is relevant with DNA detections, especially with regard to a kind of DNA detection means and detection method.
The content of the invention
It is an object of the invention to provide a kind of with very high sensitivity and reliability, production can be fast and accurately examined and determine
A kind of DNA detection means and detection method of product, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical scheme：A kind of DNA detection means, including detection means, institute
Detection means is stated provided with filtration manifold, the side of the filtration manifold is provided with computer, described multi-functional
Filter is attached by data wire with computer.
It is preferred that, the detection means is provided with multifunctional filtering rate device, and the filtration manifold was provided with
Mouth is filtered, the filtering is intraoral to be provided with filter block, and the filter block is provided with sampling test tube, and the right side of the sampling test tube is provided with control
Region processed, the control area is provided with detector.
It is preferred that, contained in the sampling test tube in phenolphthalein, physiological saline, and each test tube and contain a kind of test sample and one
Plant control substance of plant drug.
It is preferred that, a kind of detection method of DNA detection means comprises the following steps：
A, use clear water first are repeatedly gargled, and the impurity of cavity interior is cleared up；
B, a piece sampling test tube of taking-up from detection means, gently fluctuating makes physiological saline or phenolphthalein be deposited on sampling
The bottom of test tube, opens rubber stopper, sampling test tube is placed on horizontal desktop；
C, one toothpick of taking-up or spatula, the head of toothpick or spatula are attached to the inside maxilla in oral cavity, according to preceding
Rear is multiple to sliding, and oral cavity the end of last year is scraped using toothpick or spatula；
D, the mucous membrane of mouth of scraping is put into the sampling test tube for filling physiological saline or phenolphthalein and is gently mixed, by mouth
Chamber mucous membrane is immersed into reagent, until reagent becomes muddy；
E, test tube gently whipping is removed manually, solution is all deposited to the bottom of test tube, test tube is placed into detector
Filtering mouthful place, using the filter block at filtering mouth to the progress coarse filtration of DNA mixed liquors；
F, the sealing rubber plug on sampling test tube covered sampling test tube, the aperture by probe by caulking gum beyond the Great Wall
Sampling test tube is put in, the termination of probe is contacted with the detection liquid sampled；
Marker enzyme, biotin, radio isotope on G, probe, detection means make the temperature control in sampling test tube 4
DEG C, the data of probe collection are contrasted by the standard control table with the DNA detecting systems in computer, calculate contrast,
Automatically generate DNA detection tables of data.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) present apparatus can apply to various biological DNA detections, can be fast with very high sensitivity and reliability
The DNA of fast accurately calibrating product；
(2) cell tissue detected in the technical scheme using oral cavity epicuticle as DNA, using oral cavity epicuticle conduct
The cell of DNA detections can accomplish painless, and no wound is extracted convenient and swift.
Brief description of the drawings
Fig. 1 is detection means overall structure diagram of the present invention.
In figure：1st, detection means；2nd, sampling test tube；3rd, nitrocellulose filter rod；4th, filtration manifold；5th, control zone
Domain；6th, detector；7th, computer；8th, mouth is filtered；9th, filter block.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme progress applied to the present invention in example is clear, complete
Ground is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on this
Embodiment in invention, the every other reality that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example is applied, the scope of protection of the invention is belonged to.
Referring to Fig. 1, the present invention provides a kind of technical scheme：A kind of DNA detection means, including detection means 1, detection dress
Put 1 and be provided with filtration manifold 4, the side of filtration manifold 4 is provided with computer 7, and filtration manifold 4 passes through
Data wire enters 7 rows with computer and is connected；DNA detecting systems are installed on computer 7, DNA is gone out by detecting system automatic detection
Specific data, also automatically generate a DNA data monitoring table.
In the present invention, detection means 1 is provided with multifunctional filtering rate device 4, and filtration manifold 4 is provided with filtering mouth
8, filtering mouth 8 is interior to be provided with filter block 9, and filter block 9 is provided with sampling test tube 2, and the right side of sampling test tube 2 is provided with control area 5, control
Region 5 processed is provided with detector 6；Contained in sampling test tube 2 in phenolphthalein, physiological saline, and each test tube and contain a kind of test sample
With a kind of control substance of plant drug.
The detection method of the present invention, comprises the following steps：
A, use clear water first are repeatedly gargled, and the impurity of cavity interior is cleared up；
B, a sampling test tube 2 is taken out from detection means 1, gently fluctuating is deposited on physiological saline or phenolphthalein to adopt
The bottom of sample test tube, opens rubber stopper, sampling test tube 2 is placed on horizontal desktop；
C, one toothpick of taking-up or spatula, the head of toothpick or spatula are attached to the inside maxilla in oral cavity, according to preceding
Rear is multiple to sliding, and oral cavity the end of last year is scraped using toothpick or spatula；
D, the mucous membrane of mouth of scraping is put into the sampling test tube 2 for filling physiological saline or phenolphthalein and be gently mixed, will
Mucous membrane of mouth is immersed into reagent, until reagent becomes muddy；
E, test tube gently whipping is removed manually, solution is all deposited to the bottom of test tube, test tube is placed into detector 6
Filtering mouthful place, using the filter block at filtering mouth to the progress coarse filtration of DNA mixed liquors；
F, sampling test tube 2 covered by the sealing rubber plug on sampling test tube 2, probe is passed through into caulking gum beyond the Great Wall small
Hole puts in sampling test tube 2, and the termination of probe is contacted with the detection liquid sampled；
Marker enzyme, biotin, radio isotope on G, probe, detection means 1 make the temperature control in sampling test tube 2 exist
4 DEG C, the data of probe collection are contrasted by the standard control table with the DNA detecting systems in computer, calculate contrast
Degree, automatically generates DNA detection tables of data.
The present apparatus can apply to various biological DNA detections, can be quickly accurate with very high sensitivity and reliability
The DNA of true calibrating product；The cell tissue detected in the technical scheme using oral cavity epicuticle as DNA, using on oral cavity
The cell that epidermis is detected as DNA can accomplish painless, no wound, extract convenient and swift.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of DNA detection means, including detection means (1), it is characterised in that：The detection means (1) is provided with multi-functional
Filter (4), the side of the filtration manifold (4) is provided with computer (7), and the filtration manifold (4) is led to
Data wire is crossed to be attached with computer (7).
2. a kind of DNA detection means according to claim 1, it is characterised in that：The detection means (1) is provided with many work(
Energy filterability device (4), the filtration manifold (4) is provided with filtering mouth (8), and the filtering mouth (8) is interior to be provided with filtering
Block (9), the filter block (9) is provided with sampling test tube (2), and the right side of the sampling test tube (2) is provided with control area (5), institute
Control area (5) is stated provided with detector (6).
3. a kind of DNA detection means according to claim 2, it is characterised in that：The interior Sheng phenolphthalein of the sampling test tube (2),
Contain a kind of test sample and a kind of control substance of plant drug in physiological saline, and each test tube.
4. realize a kind of detection method of DNA detection means described in claim 1, it is characterised in that：Its detection method includes
Following steps：
A, use clear water first are repeatedly gargled, and the impurity of cavity interior is cleared up；
B, a sampling test tube (2) is taken out from detection means (1), gently fluctuating is deposited on physiological saline or phenolphthalein to adopt
The bottom of sample test tube, opens rubber stopper, sampling test tube (2) is placed on horizontal desktop；
C, one toothpick of taking-up or spatula, the head of toothpick or spatula are attached to the inside maxilla in oral cavity, according to front and back
It is multiple to sliding, oral cavity the end of last year is scraped using toothpick or spatula；
D, the mucous membrane of mouth of scraping is put into the sampling test tube (2) for filling physiological saline or phenolphthalein and be gently mixed, by mouth
Chamber mucous membrane is immersed into reagent, until reagent becomes muddy；
E, test tube gently whipping is removed manually, solution is all deposited to the bottom of test tube, test tube is placed into detector (6)
Filter at mouth, coarse filtration is carried out to DNA mixed liquors using the filter block at filtering mouth；
F, sampling test tube (2) covered by the sealing rubber plug on sampling test tube (2), probe is passed through into caulking gum beyond the Great Wall small
Hole puts in sampling test tube (2), and the termination of probe is contacted with the detection liquid sampled；
Marker enzyme, biotin, radio isotope on G, probe, detection means (1) make the temperature control in sampling test tube (2) exist
4 DEG C, the data of probe collection are contrasted by the standard control table with the DNA detecting systems in computer, calculate contrast
Degree, automatically generates DNA detection tables of data.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710537690.8A CN107179410A (en) | 2017-07-04 | 2017-07-04 | A kind of DNA detection means and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710537690.8A CN107179410A (en) | 2017-07-04 | 2017-07-04 | A kind of DNA detection means and detection method |
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| Publication Number | Publication Date |
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| CN107179410A true CN107179410A (en) | 2017-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710537690.8A Pending CN107179410A (en) | 2017-07-04 | 2017-07-04 | A kind of DNA detection means and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713879A (en) * | 2021-09-04 | 2021-11-30 | 胡彦平 | Intelligent veterinary drug test tube rack |
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| CN101925677A (en) * | 2007-11-29 | 2010-12-22 | 奎斯特诊断投资公司 | Detect the assay method of genetic abnormality in the genomic nucleic acids |
| CN103403190A (en) * | 2011-03-04 | 2013-11-20 | 株式会社钟化 | Nucleic acid detection method, and device and kit for use in same |
| CN106596227A (en) * | 2016-12-27 | 2017-04-26 | 上海中优医学检验所有限公司 | Preparation method of carrier original for nucleic acid sample collection |
| CN106754343A (en) * | 2017-01-12 | 2017-05-31 | 武汉菲思特生物科技有限公司 | DNA sequencing apparatus and system based on pyrosequencing |
-
2017
- 2017-07-04 CN CN201710537690.8A patent/CN107179410A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030082209A (en) * | 2002-04-17 | 2003-10-22 | 최강석 | Apparauts for DNA GEL documentation using Scanner |
| CN101925677A (en) * | 2007-11-29 | 2010-12-22 | 奎斯特诊断投资公司 | Detect the assay method of genetic abnormality in the genomic nucleic acids |
| CN103403190A (en) * | 2011-03-04 | 2013-11-20 | 株式会社钟化 | Nucleic acid detection method, and device and kit for use in same |
| CN106596227A (en) * | 2016-12-27 | 2017-04-26 | 上海中优医学检验所有限公司 | Preparation method of carrier original for nucleic acid sample collection |
| CN106754343A (en) * | 2017-01-12 | 2017-05-31 | 武汉菲思特生物科技有限公司 | DNA sequencing apparatus and system based on pyrosequencing |
| CN106754313A (en) * | 2017-01-12 | 2017-05-31 | 武汉菲思特生物科技有限公司 | Nucleic acid sequencing apparatus and system |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113713879A (en) * | 2021-09-04 | 2021-11-30 | 胡彦平 | Intelligent veterinary drug test tube rack |
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Application publication date: 20170919 |
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