KR20030082209A - Apparauts for DNA GEL documentation using Scanner - Google Patents

Abstract

PURPOSE: An apparatus for DNA gel documentation using a scanner is provided, thereby rapidly, easily and cheaply carrying out the electrophoresis and documentation. CONSTITUTION: An apparatus for DNA gel documentation comprises an electrophoresis device; an ultraviolet lamp for irradiating UV to DNA gel; a diffuser for evenly distributing the UV intensity; an UV filter for filtering the UV from the diffuser; an optical filter for blocking the DNA fragment free light released from dyed DNA irradiated by UV; a scanner for scanning the light passed through the optical filter via one dimensional CCD sensor; and a computer and a video monitor outputting the image from the scanner.

Description

Scanner-type DNA gel documentation device {Apparauts for DNA GEL documentation using Scanner}

The present invention relates to a hermetic DNA component analysis system using a deoxyribonucleic acid (hereinafter abbreviated as 'DNA') electrophoretic device, and more specifically, an electrophoretic device in a hermetically sealed container using a filter for passing only a specific wavelength of light. And a gel (GEL) in which a DNA sample is injected into an electrophoretic apparatus and irradiated with ultraviolet rays to convert the DNA fragment image to a digital image using a scanner that moves one-dimensionally. To a stationary device.

In general, DNA component analysis is the most basic necessity in the fields of biotechnology, microbiology, genetics, molecular biology, etc., to determine how many kinds of fragments are contained in a DNA sample to be analyzed.

To this end, an electrophoresis method is used, which utilizes a property that DNA fragments injected into an agarose fountain formed in an agarose gel in an electrolyte solution physically react and move by an externally applied current. When the electrophoresis is completed, the DNA fragments are moved according to their weight, and the UV rays are irradiated after soaking agarose gel in a dye solution called Ethidium Bromide (hereinafter abbreviated as 'EtBr'). EtBr near the fragments receives energy and emits an orange light in the visible region. GEL documenting system refers to a device that converts the orange pattern into an image signal and outputs it to a thermal radiation printer, or converts it into a digital image and stores it in a computer.

Commonly used GEL documentation systems consist of UV lamps with UV filters, CCD cameras, printers and computers. The DNA sample exposed to ultraviolet light emits orange light through EtBr, which is converted into an image by a camera. The problem here is the intensity of the orange light and the sensitivity of the camera. Since the light intensity is usually very small, you need to use a dark room that cuts off the external light to get a clearer image, and then adjust the exposure time of the camera. Use

The GEL documenting system and the electrophoretic device, which are image input devices used in combination with the illuminator, have distinct functions, but can be understood as a continuous operation in terms of DNA analysis. Therefore, implementing such an electrophoretic device and a GEL documentation system as a one-body device has several advantages. First, the overall DNA analysis can be performed in one place, which can yield a lot of efficiency gains. Second, the electrophoretic device and the GEL documenting device do not need to be purchased separately, so that one device can replace the space where two devices need to be installed, thereby improving the working environment.

1 is an explanatory view of the operation of a DNA documenting apparatus using an electrophoretic apparatus according to the prior art, the method A shows that the transilluminator is installed at the bottom of the electrophoretic device, the method B is a transilluminator electrophoretic device It is installed at the top of the. Both methods can produce DNA distribution images of agarose gels in the electrolyte solution (11). The light generated by the ultraviolet lamps 12 and 14 passes through the optical filters 16 and 18 which only pass ultraviolet rays of a desired wavelength and is irradiated to the agarose gel 90. DNA fragments stained with EtBr emit orange light in response to ultraviolet rays and are converted into image signals through the camera 22.

U. S. Patent 4,657, 655 describes a method of combining an electrophoretic device, an illuminator, and a camera as one device.

Cameras used as video input devices usually use things like polaroid cameras and two-dimensional CCD cameras. Polaroid cameras have excellent film sensitivity, but the disadvantage is that the quality of the image depends largely on the maintenance cost and the experience of the person taking the film.

On the other hand, CCD cameras have lower resolution and lower sensitivity of CCD sensors than Polaroid cameras, but it is possible to maintain constant image quality at all times. There is a merit that it costs less.

However, the fundamental problem with the image input device is that the intensity of the orange light emitted by the transferred DNA fragments is very weak when ultraviolet rays are irradiated onto the agarose gel into which the DNA sample is injected. In order to compensate for this, the most commonly used method is to adjust the exposure time of the camera for a long time. However, if the exposure time is extended, the light is partially saturated, and thus the overall sharpness of the captured image is reduced.

In addition, the light emitted from the agarose gel in the electrolyte solution arrives at the camera only after passing through the agarose gel and the electrolyte solution, reducing the size of the agarose in the process. There was a problem that the result is worse than taking the gel separately and shooting the image directly with the camera.

The present invention is to solve the above problems of the prior art, the purpose of the electrophoresis by the electrophoresis device using an airtight container using a sealed container to analyze the DNA component composition, or irradiated with ultraviolet rays after the end of electrophoresis The present invention provides a scanner-type DNA gel document device that converts the distribution of DNA components, which are represented by the same, into an image through a scanner-type image input device and stores or outputs the same.

1 is a block diagram of a DNA gel document device using an electrophoresis device according to the prior art,

2 is a block diagram of a scanner-type DNA gel document device according to an embodiment of the present invention,

3 is an exploded perspective view of an electrophoretic apparatus according to an embodiment of the present invention,

4 is an exploded perspective view of an electrophoretic apparatus and its peripheral according to an embodiment of the present invention,

5 is an exploded perspective view showing a scanner-type image input apparatus according to an embodiment of the present invention;

6 is an exploded perspective view of a scanner-type DNA gel document device according to an embodiment of the present invention,

7 is a block diagram of a control device for operating the DNA gel documentation system according to an embodiment of the present invention,

8 is an exploded perspective view of a scanner-type DNA gel document device according to another embodiment of the present invention,

9 is a view showing an optical system of a scanner-type DNA gel document device according to another embodiment of the present invention.

<Description of Symbols for Major Parts of Drawings>

100, 115: Case 101: Video monitor

102: computer 110: electrophoresis device

113, 114: electrode 116, 126: cover

117: guide groove 121: power switch

122: power indicator 123: electrophoretic indicator

124: timer switch 125: insertion hole

126: cover 127: pin

131, 191: ultraviolet lamp 132: diffuser

133, 194 UV filter 134 agarose gel

135: optical filter 141: plate

142, 143: groove 144, 145: terminal

146: vertical plane 147,148: hole

149, 151 switch 153

160: scanner 161: prism

162: optical lens module 163: filter

164: CCD sensor 166, 174: support

168, 170: bar 180: microprocessor

181: user interface unit 182: electrophoretic control unit

183: UV lamp driving unit 184: scanner control unit

185: computer interface unit 186: video signal generator

187: program memory 188: image memory

189: speaker drive unit 190, 195: ultraviolet irradiation unit

192: reflector plate 196: electrolyte solution

The scanner-type DNA documenting device according to the present invention for achieving the object of the present invention is an electrophoresis device for electrophoresis gel gel injected with a DNA sample in a sealed container, DNA fragments electrically separated by the electrophoresis device In the DNA gel document system for documenting the image of the human body, an ultraviolet lamp for irradiating ultraviolet rays to the gel injecting the electrophoretic DNA sample of the electrophoresis device, and the intensity of the ultraviolet light irradiated from the ultraviolet lamp Diffuser to distribute uniformly, UV filter for optimizing the reaction of dyeing material by passing only the specific wavelength of ultraviolet light passing through the diffuser, and light emitted by the dye DNA in response to ultraviolet light passing through the UV filter. The optical filter to block the part without DNA fragments, and the light passing through the optical filter through the one-dimensional CCD sensor And a computer for detecting an image signal, a computer for outputting the image detected by the scanner, and a video monitor.

Another method for achieving the object of the present invention is an electrophoresis device for electrophoresis gel gel injected DNA sample in a sealed container, and a DNA document for documenting the images of DNA fragments electrically separated by the electrophoresis device In the mention system, the scanner including a prism, a lens module, a filter and a CCD sensor to detect an image of the gel injected with the DNA sample while moving the lower end of the electrophoretic device, and fixed to the both sides of the prism of the scanner Ultraviolet irradiation means for irradiating the ultraviolet rays to the gel through the electrophoretic device, characterized in that it comprises a computer and a video monitor to output the image detected by the scanner.

The present invention made as described above will be described in detail with reference to the accompanying drawings.

Figure 2 is a schematic configuration diagram of a DNA gel document device of the scanner method according to an embodiment of the present invention, an electrophoresis device 110 for electrophoresis of the gel injected with the DNA sample in a sealed container, and the electrophoresis A video monitor for inserting the device 110 into a sealed case 100 including a scanner for scanning an image of an electrophoretic gel, and connecting to one side of the case 100 to output an image detected by the scanner. 101 and the computer 102.

Here, the case 100 has a power switch 121, a power indicator 122, an electrophoretic indicator 123, a timer switch 124, the insertion hole 125 for the insertion and removal of the electrophoretic device on the front. It includes a power supply connection portion, a video monitor connection portion for outputting a DNA fragment image and a computer connection portion on the back and the extraction pin 127 for taking out the inserted electrophoretic device (110).

6 is a separation configuration diagram of a scanner-type DNA gel document device according to an embodiment of the present invention, an ultraviolet lamp 131 for irradiating ultraviolet rays to a gel injecting an electrophoretic DNA sample of the electrophoretic device, A diffuser 132 for uniformly distributing the intensity of the ultraviolet light emitted from the ultraviolet lamp 131 and an ultraviolet filter 133 for optimizing the reaction of the dyeing material by passing only a specific wavelength of ultraviolet light passing through the diffuser 132. ), An optical filter 135 for blocking a portion without DNA fragments from light emitted by the dyed DNA in response to the ultraviolet light passing through the ultraviolet filter 133, and light passing through the optical filter 135. The scanner 160 scans through a 1D CCD sensor and detects an image signal.

The scanner 160 includes a prism 161, an optical lens module 162, a filter 163, and a one-dimensional CCD sensor 164 below the optical filter 135.

The operation according to the embodiment of the present invention configured as described above will be described in more detail with reference to FIGS. 2 to 9 as follows.

First, the electrophoretic method used in the present invention uses the orange light generated from the agarose gel, that is, the light penetrates the bottom of the sealed container used for electrophoresis from the secondary light source generated by dyeing the DNA fragments. You will not. In addition, the use of an optical filter to filter out unwanted light generated in the absence of DNA can prevent the conversion of unwanted parts into a video signal. The image input device using the scanner method is capable of producing an image signal having a very high resolution compared to the CCD camera used by conventional methods, and partly excessive because the subject is photographed as a continuous one-dimensional image having a narrow width. It is easy to prevent saturation of the video signal due to exposure.

On the other hand, the present invention is an electrophoresis device for analyzing the composition of DNA components and ultraviolet rays after the electrophoresis is in progress or finished, by using a scanner to image the distribution of DNA components appearing on the electrophoresis device after the computer or video Video output through the monitor. Integrate such an electrophoresis device and an image processing device including a scanner to proceed with gel documentation.

2 is an external view of a scanner-type DNA documenting apparatus according to an embodiment of the present invention, the hermetic electrophoresis apparatus 110 in which an agarose gel in which an electrolyte solution and a DNA sample are injected is installed. It is inserted into the interior through the insertion hole 125 provided in the front.

When the electrophoretic device 110 is completely inserted into the system, the cover 126 covers the front surface of the case 100 to prevent light from entering the system.

When removing the inserted electrophoretic device 110 out of the case 100, the pressing pin 127 installed on the rear of the case 100 is pressed.

The power switch 121, the power indicator 122, the electrophoretic device indicator 123, the timer switch 124 is installed at the lower end of the front surface of the case 100. The power switch 121 is used to turn on / off the power of the system, and the power indicator 122 indicates whether power is normally supplied. The electrophoretic indicator 123 displays whether the electrophoretic apparatus 110 is normally installed in the system.

The timer switch 124 is for adjusting the time for performing electrophoresis, and when the timer switch 124 is set to a desired time, electrophoresis is started. When the set time passes, the power switch 121 is automatically turned off.

If you want to see the image of the DNA fragments that are spread during the electrophoresis or terminated, it can be confirmed as an image by connecting the video monitor 101 or the computer (102). When the computer is connected to the system, all operations of the system can be controlled by the computer 102.

3 shows a schematic diagram of an electrophoretic apparatus 110 which is one of the components of the present invention. The electrophoretic apparatus 110 is formed as a sealed container and has a structure in which the electrode is installed at both ends thereof, and has a structure in which the cover 116 can be inserted into the upper portion of the container. In addition, both sides of the electrophoretic device 110 is formed with a guide groove 117 of the straight type, it allows to enter the correct position when inserted into the system. The agarose gel is formed at the center bottom of the electrophoresis device 110 and then sealed with a cover 116 after filling the electrolyte solution. Electrodes 113 and 114 installed on both bottoms of the sealed electrophoretic apparatus 110 are conductors for passing electricity, and electrophoresis is performed by applying voltages of the positive and negative electrodes, respectively.

In addition, an optically transparent glass is installed on the bottom center of the electrophoretic device 110 and the center of the cover 116, or an ultraviolet filter 133 for passing light having a specific wavelength is used. When the ultraviolet filter 133 is used, the cover 116 uses an optical filter 133 that passes ultraviolet rays, and an optical filter 135 that passes orange light is usually used for the bottom portion.

Here, each filter can be replaced by various special filters according to a given purpose. The electrodes 113 and 114 and the filters 133 and 135 are manufactured to be completely coupled to the body of the case 115 of the electrophoretic apparatus 110, and the electrolyte solution filled therein does not flow out. Keep it closed.

4 is an overall configuration diagram of the electrophoretic device 110, the center there is a plate 141 for supporting the sealed electrophoretic device 110 is inserted. When the hermetic electrophoresis device 110 is inserted into the center of the plate 141, grooves 142 and 143 are dug in contact with the electrodes 113 and 114 installed at both bottoms of the electrophoresis device 110. The terminals 144 and 145 for supplying electricity through the grooves 142 and 143 are installed. In addition, three holes are formed in the inner vertical surface 146 of the plate 141. Switches 149 and 152 are installed in the two holes 147 and 148 at both ends. When the electrophoretic device 110 is correctly positioned in the plate 141, the two switches 149 are provided. 151 is pressed. Thus, the system can know through this switch whether the electrophoretic device 110 is correctly inserted into the plate 141. The hole 152 in the center is for the pin 127 used to push the electrophoretic device 110. When the pin 127 installed at the rear of the system is pressed as shown in FIG. 1, the pin 127 is inserted through the center hole 152, and consequently, the electrophoretic device 110 is formed by the pin 127. Will act to push. Ultraviolet lamps 131 are installed above the plate 141. An inclined barrel 153 is installed in the middle so that all the light generated from the ultraviolet lamp 131 can be emitted toward the electrophoretic device 110.

Here, the ultraviolet lamp 131 may be composed of two or more kinds of lamps, all operations of turning on and off the ultraviolet lamp 131 is made through the control device of the system. Ultraviolet rays generated from the UV lamp 131 installed on the upper side are reflected on the agarose gel installed inside the electrophoretic apparatus 110, and when the EtBr-stained DNA fragments emit orange light, an image input device installed under the container. Through the video signal is converted.

5 is an exploded perspective view showing a scanner-type image input device which is one of the components of the present invention.

The video input device is provided on the bottom surface of the plate 141 shown in FIG. When the DNA fragments emit orange light in response to the ultraviolet light generated by the ultraviolet lamp 131, first, the DNA fragments are integrated through the prism 161 and then pass through the optical lens module 162 to the image signal through the one-dimensional CCD sensor 164. Is converted.

These optical lens modules 162 are fixed through the support 166 that surrounds the outside. Holes are formed in both sides of the support surrounding the optical lens module 162, and the rods 168 and 170 can be reciprocated to both sides through the holes.

Therefore, the two-dimensional distribution image of the DNA fragments emitting orange light is made by moving the optical module that produces the one-dimensional image signal in the vertical direction. The scanner mechanism as described above is coupled to the bottom support 174 and the bottom support 174 is connected to the plate 141 again.

Figure 6 shows the configuration of the optical system used in the electrophoretic device and the image input device which is a component of the present invention. This optical system can be divided into two stages.

The first step is an optical system that allows the ultraviolet rays generated from the ultraviolet lamp 131 to react with EtBr, a dyeing material injected into the agarose gel.

The second step is the optical system required for the orange light generated at EtBr to become the secondary light source and reach the CCD sensor 164.

In both stages, the optical filter is involved and the required characteristics vary depending on the position. In addition, the combination of the filters used to determine the characteristics of the image signal converted by the CCD sensor 164.

First, ultraviolet light generated by the ultraviolet lamp 131 passes through the diffuser 132. The purpose of using the diffuser 132 is to allow the intensity of the ultraviolet rays irradiated on the agarose gel to be evenly distributed. Since the ultraviolet lamp 131 is not a continuous light source, the intensity of light at the position where the lamp is located is the strongest and the intensity of light is weakened at the position where the lamp is not. Therefore, in order to solve this disadvantage, the direction of the ultraviolet rays generated from the ultraviolet lamp 131 is to be random (random), the diffuser 132 has such a function.

Ultraviolet rays irradiated to the diffuser 132 are randomly changed in the direction thereof. Therefore, it may be assumed that the intensity of ultraviolet light passing through the diffuser 132 is uniform. Ultraviolet light that has passed through the diffuser 132 then passes through the ultraviolet filter 133. Since the ultraviolet rays generated from the ultraviolet ray lamp 131 have a fairly wide range of wavelengths, EtBr, which is a dyeing material used to dye agarose gel, contains wavelengths other than the most strongly reacted ultraviolet rays.

DNA is destroyed by prolonged exposure to ultraviolet rays, so unnecessarily irradiating with unwanted ultraviolet rays will adversely affect the experimental results.

Therefore, it is necessary to use a filter for passing only ultraviolet rays of a desired wavelength. The ultraviolet light passing through the ultraviolet filter 133 is irradiated onto the agarose gel 134 contained in the electrolyte solution, and DNA fragments stained with EtBr emit orange light in response to the ultraviolet light.

On the other hand, a portion of the agarose gel 134 without DNA fragments may emit light different from orange light in response to the ultraviolet rays.

Therefore, when the distribution image of DNA fragments stained on EtBr is made, the strength of the unwanted portions affects the DNA analysis result.

The orange light generated from the agarose gel 134 irradiated with ultraviolet rays passes through the optical filter 135 installed below. At this time, the optical filter 135 to be used should satisfy the property of best passing the light emitted by the DNA stained on EtBr.

By using such an optical filter 135, unnecessary light generated in the agarose gel 134 in the portion without the DNA fragment can be blocked. Light passing through the optical filter 135 is transmitted to the one-dimensional CCD sensor 164 through the prism 161 and the optical lens module 162 constituting the scanner mechanism 160. One-dimensional image signal of the DNA fragments stained can be made through the one-dimensional CCD sensor 164. When the image signal is continuously generated while moving the CCD sensor 164 little by little in the vertical direction, two-dimensional images are finally obtained. .

A separate filter 163 may be provided between the optical lens module 162 and the CCD sensor 164 to adjust the characteristics of the DNA fragment image.

Table 1 shows the types and characteristics of light generated in the optical system shown in FIG.

Kinds characteristic lambda (u) UV rays generated from UV lamp lambda _d (u) UV rays through the diffuser lambda _d (u _o) UV light of a specific wavelength passing through the UV filter lambda _d (u _s) Visible light from undyed areas lambda _d (o) Orange light from the stained part of EtBr

FIG. 7 is a block diagram of a control device for operating the DNA documenting system illustrated in the present invention. When the system is powered on, the operating software in the program memory 187 is loaded into the microprocessor 180. Is executed, and the system enters the initial state.

When the user operates the timer switch 124 installed on the front of the system to set the electrophoresis time, the microprocessor 180 drives the electrophoresis apparatus 110. Prior to applying power, the electrophoresis control unit 182 detects whether the electrophoretic device 110 is inserted into the system through the state of the insertion switch. Electrophoresis does not start if the electrophoretic device 110 is not inserted.

If the operation time is set by the timer switch 124 for electrophoresis in the state in which the electrophoretic apparatus 110 is not inserted, the microprocessor 180 detects the operation time through the user interface (I / F) unit. By flashing the indicator light 123 installed on the front of the system at regular time intervals or by generating a warning sound through the speaker driver 189 to the speaker SPK, the user is notified.

When electrophoresis is completed, the scanner 160 is automatically driven through the scanner controller 184 to make a DNA distribution image. In this case, the UV lamp driver 183 and the scanner controller 184 automatically control the movement of each of the ultraviolet lamps 131 and the scanner 160 in the order determined by the microprocessor 180.

Here, when the user wants to check the electrophoresis process, the image of the gel is scanned at a predetermined time interval by a timer.

When the image input is completed, it is stored in the image memory 188, and at the same time is converted into a video signal through the video signal generator 186 and then display the DNA distribution image through the connected video monitor 101.

If the computer 102 is connected, the corresponding image data is transmitted to the computer 102.

In addition, all operations of the system may be controlled through a program running on the computer 102. That is, when the computer 102 transmits a control command to the system through the computer interface unit 185, the microprocessor 180 interprets the command and executes an operation corresponding to the command.

As such, when the computer 102 is externally connected, the input image may be transmitted to the computer 102 at any time through the computer interface (I / F) unit 185. In addition, while the electrophoresis is in progress, it is possible to input the image and to analyze the continuously photographed image.

8 is a configuration diagram of a scanner-type DNA documenting apparatus according to another embodiment of the present invention, which moves underneath the electrophoresis apparatus 110 and moves a prism to detect a fragment image of the gel injecting the DNA sample. 161, the scanner 160 including the optical lens module 162, the filter 163, and the CCD sensor 164, and the electrophoretic apparatus 110 by being fixed to both sides of the prism 161 of the scanner 160. Ultraviolet irradiation unit 190 (195) for irradiating the ultraviolet rays to the agarose gel 135 through the bottom of the; and the computer 102 and the video monitor 101 for outputting the image detected through the scanner 160 It consists of.

Herein, each of the ultraviolet irradiation units 190 and 195 disposed on both sides of the prism 161 passes only the UV lamp 191 for emitting ultraviolet rays and only a component of a specific region among the ultraviolet rays emitted from the UV lamp 191. An ultraviolet filter 194 and a reflective surface 192 surrounding the ultraviolet lamp 191 so that ultraviolet rays emitted from the ultraviolet lamp 191 are irradiated through the ultraviolet filter 194.

As shown therein, another embodiment according to the present invention, as shown in Figs. 8 and 9, does not place an ultraviolet (UV) light source above the electrophoretic device 110, but both sides of the scanner mechanism 160 It is a figure which shows the Example provided in. Under this type of arrangement, the bottom surface of the electrophoretic apparatus 110 should be optically transparent, and the light source moves with the scanner mechanism 160.

The ultraviolet light source installed on the side of the scanner mechanism 160 includes an ultraviolet lamp 190, 191, a reflective surface 192 surrounding the lamp, and an ultraviolet filter 194 provided on the upper surface. The reflective surface 192 surrounding the ultraviolet lamp 191 is to reflect the light generated from the ultraviolet lamp 191 to be emitted through the ultraviolet filter 194 installed on the upper surface. That is, since the light generated from the ultraviolet lamp 191 is spread out in the 360 ° direction, all the light is refracted in the upward direction in which the DNA sample is placed, thereby increasing the efficiency.

In addition, the electrophoretic device 110, including the coupling device for electrophoresis and the scanner mechanism 160 to be used separately, the gel electrophoresis elsewhere using the scanner mechanism 160 to image To obtain.

9 shows an optical system applied to the scanner mechanism given in FIG. Among the ultraviolet rays generated by the ultraviolet lamp 191, only the ultraviolet component reacting with EtBr passes through the ultraviolet filter 191 and is irradiated to the DNA sample 135 contained in the electrolyte solution 196 through the bottom of the electrophoretic apparatus 110. do. DNA stained on EtBr emits orange light in response to the ultraviolet rays, and the light is converted into an image signal through a scanner mechanism 160 installed at the lower end of the electrophoretic apparatus 110.

The orange light passing through the prism 161 installed at the lower end of the electrophoretic device 110 passes through the optical lens module 162 and then passes through the filter 163 which passes only the orange light, and then is projected onto the CCD sensor 164. . As such, when the ultraviolet irradiation unit 190 is used in combination with the scanner mechanism 160, the ultraviolet ray lamp 191 may be used because the ultraviolet ray is irradiated only on a narrow area to obtain a DNA image. That is, even if the UV lamp 191 of low output is used, there is no fear of deterioration of the image quality. In addition, since the ultraviolet irradiation unit 190 and 195 may be installed at both sides of the prism 161 of the scanner, ultraviolet lamps using two kinds of wavelengths may be installed.

Although a preferred embodiment according to the present invention has been described above, it can be modified in various configurations according to the position of the scanner or the number of ultraviolet lamps, as well as various modifications such as an integrated or detachable type of electrophoretic device and scanner. Therefore, it will be understood by those skilled in the art that various modifications and variations can be made without departing from the scope of the claims of the invention.

As described above, the scanner-type DNA gel document device according to the present invention uses an image input device using a scanner-type mechanism instead of a CCD camera, thereby reducing the required space as much as possible. In addition, even if the gel size is large, scanning with a scanner has an effect that can be easily imaged, and also images having a wide range of resolutions such as 120 to 2400 dpi (dots per inch) can be obtained. By adjusting the exposure time and resolution, there is an effect that can improve the sharpness of the image. In addition, by integrating the electrophoresis device and gel documentation system, electrophoresis and documentation work can be carried out in a consistent process through a single device, so experiments are quick, easy, and the system production cost can be reduced. There is.

Claims (11)

In an electrophoresis device for electrophoresis of the gel injected with the DNA sample in a sealed container, and a DNA gel documenting system for documenting the image of the DNA fragments electrically separated by the electrophoresis device, An ultraviolet lamp for irradiating ultraviolet rays to the gel into which the electrophoretic DNA sample of the electrophoretic device is injected; A diffuser for uniformly distributing the intensity of the irradiated ultraviolet rays from the ultraviolet lamp; An ultraviolet filter for optimizing the reaction of the dyeing material by passing only a specific wavelength of ultraviolet light passing through the diffuser; An optical filter blocking a portion of the DNA fragment in the light emitted by the dyed DNA in response to the ultraviolet light passing through the ultraviolet filter; A scanner for detecting an image signal by scanning the light passing through the optical filter through a one-dimensional CCD sensor; And And a computer and a video monitor outputting the image detected by the scanner. According to claim 1, wherein the upper and lower surfaces of the electrophoretic device is a die-gel documenting apparatus of the scanner method, characterized in that the sealing with an ultraviolet filter and an optical filter, respectively. According to claim 1, wherein the electrophoretic device and each part is embedded in a sealed container so that light does not pass, and the power switch, power indicator, electrophoretic indicator, timer switch and electrophoretic device on the outside front An insertion and detachable insertion hole, the outer back of the scanner-type DNA gelation apparatus comprising a power supply means, a video monitor connection means for outputting a DNA fragment image and a computer connection means. The method of claim 1, wherein the scanner device A scanner-type DNA documenting apparatus which scans an image of a gel at a predetermined interval under the control of the timer and converts the gel conversion process into an image. The method of claim 4, wherein the scanner device A scanner-type DNA gelation apparatus comprising a prism, an optical lens module, and a 1-dimensional CCD sensor under the optical filter. The apparatus of claim 4, further comprising a filter for controlling the characteristics of the DNA fragment image between the optical lens module and the CCD sensor in addition to the scanner device. . The method of claim 1, further comprising a computer interface unit for transmitting the image data detected by the scanner to a host computer and a video signal generator for converting the image data detected by the scanner to a video monitor. Scanner-type DNA Documenting Device. The scanner according to claim 1, comprising a diffuser at the bottom of the ultraviolet lamp that emits ultraviolet rays, an ultraviolet filter at the bottom thereof, a gel containing an electrolyte solution at the bottom thereof, an optical filter at the bottom thereof, and a scanner at the bottom thereof. DNA documentation device. In an electrophoresis device for electrophoresis gel gel injected DNA sample in a sealed container, and a DNA documenting system for documenting images of DNA fragments electrically separated by the electrophoresis device, A scanner including a prism, an optical lens module, a filter, and a CCD sensor to move the lower end of the electrophoretic device and detect an image of the gel injecting the DNA sample; Ultraviolet irradiation means for irradiating the ultraviolet rays to the gel through the lower end of the electrophoretic device by fixing to both sides of the prism of the scanner; And And a computer and a video monitor outputting the image detected by the scanner. 10. The method of claim 9, wherein each ultraviolet irradiation means located on both sides of the prism is An ultraviolet lamp emitting the ultraviolet rays; An ultraviolet filter for passing only components of a specific region of ultraviolet rays emitted from the ultraviolet lamp; And And a reflecting surface surrounding the ultraviolet lamp so that the ultraviolet light emitted from the ultraviolet lamp is irradiated through the ultraviolet filter. The method of claim 9, wherein the ultraviolet irradiation direction of each of the ultraviolet irradiation means located on both sides of the prism is formed so as to face the intersection of the center of the prism and the gel surface to detect the image by the prism. Documentation device.