CN107176873A - Biological organic fertilizer and its preparation method and application - Google Patents
Biological organic fertilizer and its preparation method and application Download PDFInfo
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- CN107176873A CN107176873A CN201710322522.7A CN201710322522A CN107176873A CN 107176873 A CN107176873 A CN 107176873A CN 201710322522 A CN201710322522 A CN 201710322522A CN 107176873 A CN107176873 A CN 107176873A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a kind of biological organic fertilizer and its preparation method and application, biological organic fertilizer is inoculated into mixing organic fertilizer using the zymotic fluids of bacillus IBFCBF 1, is prepared through solid fermentation；Mixing organic fertilizer includes cow dung organic fertilizer and amino acid fertilizer.Its preparation method is：Sheep manure organic fertilizer and amino acid fertilizer are mixed to get mixing organic fertilizer；The zymotic fluids of bacillus IBFCBF 1 are inoculated into mixing organic fertilizer progress solid fermentation and obtain biological organic fertilizer.The biological organic fertilizer of the present invention can effectively control the generation of capsicum epidemic disease, improve yield of hot pepper, can be applied to capsicum epidemic disease preventing and treating and capsicum volume increase.
Description
Technical field
The present invention relates to technical field of biological fertilizer, more particularly to a kind of biological organic fertilizer and its preparation method and application.
Background technology
Capsicum, alias：Long red pepper, long capsicum, green pepper, bell pepper, Latin literary fame：Capsicum annuum L., Solanaceae,
Capsicum 1 year or limited herbaceos perennial.Capsicum is one of fruits and vegetables main in the world, is accounted in human food habits
There is very important status, countries in the world, which have, generally plants, in China Sichuan, Guizhou, Hunan, Yunnan, Shaanxi, Henan（Wash rice
Chuan Xian）, the province such as Hebei province and the Inner Mongol have large area plantation.Capsicum epidemic disease is by phytophthora blight of pepper（Phytophthora capsici Leon）The Ragnarok venereal disease evil that the caused time of infertility can fall ill.Based on the disease is propagated with soil and rainwater,
In addition to causing the dead seedling of large area, it can also cause withered blade, fruit rot, cane a variety of symptoms such as necrotic plaque occur, cause
The upper a large amount of underproduction of capsicum production, or even total crop failure, seriously govern the development of capsicum industry and the raising of economic benefit.At present, state
The inside and outside preventing control method to capsicum epidemic disease is varied, mainly including the use of chemical agent, breeding resistant variety, biological control
Deng.But due to phytophthora blight of pepper host extensively, the generally existing in soil, and be also easy to produce the resistance to the action of a drug to bactericide, is largely used
Agricultural chemicals can cause the residues of pesticides of environmental pollution and fruit.The resistance of capsicum blight-resistant germplasm is the number by controlled by multiple genes simultaneously
Character is measured, its transformation difficulty is very big, and mostly moderate resistance or disease tolerant variety, few high anti-kinds, let alone there are immunological species.
Therefore, the prevention and control to capsicum epidemic disease do not have a kind of relatively good prevention and controls temporarily.
The feces of livestock and poultry after the general on-site incineration of agricultural crop straw, large-scale cultivation in still further aspect, agricultural production
Throw aside everywhere, substantial amounts of nutrients resource is lost in outside arable soil system, not only severe contamination environment, also greatly
Wasting as organic fertilizer and the organic resources of biological organic fertilizer raw materials for production, can be unfavorable for the sustainable development of China's agricultural
Exhibition.These agricultural organic wastes are prepared into during biological organic fertilizer is manured into soil again, agricultural organic waste will be effectively solved
The problem of, it can not only turn waste into wealth, realize resource in utilization, but also can purify and improve agroecological environment.Therefore,
These agricultural organic wastes are further used as soil beneficial microorganism carrier by hot fermentation into the organic fertilizer of high-quality,
Preparing will have broad application prospects as the biological organic fertilizer with clear and definite function.
The problem of in correlation technique, effective solution is not yet proposed at present.
The content of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art effectively to control capsicum epidemic disease there is provided one kind
The generation of disease, the biological organic fertilizer for improving yield of hot pepper, additionally provide the preparation method of the biological organic fertilizer and in capsicum epidemic disease
Application in disease preventing and treating and capsicum volume increase, the not only function with organic fertilizer, and biocontrol microorganisms contained therein pass through a variety of promote
Raw and Biocontrol Mechanism works, so as to reach growth-promoting or prevent and treat the effect of capsicum epidemic disease.
In order to solve the above technical problems, the present invention provides a kind of biological organic fertilizer, the biological organic fertilizer is used gemma
Bacillus IBFCBF-1 zymotic fluids are inoculated into mixing organic fertilizer, are prepared through solid fermentation；The mixing organic fertilizer includes ox
Excrement organic fertilizer and amino acid fertilizer.
Above-mentioned biological organic fertilizer, it is preferred that in the biological organic fertilizer, bacillus IBFCBF-1 viable count is
3.0×108More than cfu/g, the content of nitrogen is 20mg/kg~28mg/kg；The content of phosphorus is 12mg/kg~18mg/kg；Potassium
Content is 28mg/kg~32mg/kg, organic matter >=40%.
As a total technical concept, present invention also offers a kind of preparation method of above-mentioned biological organic fertilizer, including
Following steps：
S1, sheep manure organic fertilizer and amino acid fertilizer be mixed to get mixing organic fertilizer；
S2, by bacillus IBFCBF-1 zymotic fluids by volumn concentration be 15~20% be inoculated into mixing organic fertilizer consolidated
Body fermentation obtains biological organic fertilizer.
Above-mentioned preparation method, it is preferred that in the S1 steps, the volume of the sheep manure organic fertilizer and amino acid fertilizer
Than for 5: 5；
And/or, in the S2 steps, ferment 7~8d in the solid fermentation process in the environment of temperature≤55 DEG C, fermented
Daily turning obtains the biological organic fertilizer of the IBFCBF-1 containing bacillus for 1~2 time in journey, and its water content is below 30%.
Above-mentioned preparation method, it is preferred that the preparation method of the sheep manure organic fertilizer is：
S1-1, the fresh sheep manure by 80wt%~85wt%, 10wt%~12wt% system chaff, 2wt%~5wt% sawdust, 3wt%~
5wt% rapeseed meal, 0.3wt% fermenting agent is uniformly mixed so as to obtain composting material；
S1-2, by the composting material regulation water content be 50%~60% after, beat heap and obtain heap body；
S1-3, the heap body is subjected to compost fermentation 12d~18d.
It is further preferred that the compost fermentation time in summer is 12d~15d；The compost fermentation time in winter is 15d~18d.
Above-mentioned preparation method, it is preferred that in the S1-2 steps, the height of the heap body is 1.5m~2m, and width is
2.0m~2.5m；And/or, in the S1-3 steps, when the heap body is warming up to 60 DEG C~65 DEG C, turning is carried out, is turned over daily
Heap 1~2 time；When temperature drop is to 40 DEG C~45 DEG C, every turning in two days is once.
Above-mentioned preparation method, it is preferred that 10% >=N+P in the amino acid fertilizer2O5+K2O >=6%, organic matter >=
40%, 8% >=amino acid >=5%.
Above-mentioned preparation method, it is preferred that in the S2 steps, the bacillus IBFCBF-1 zymotic fluids are using following
Method is prepared：Bacillus IBFCBF-1 inoculations are subjected to liquid fermentation into fermentation medium, gemma is formed；Institute
Stating liquid fermentation medium includes 10.1gL-1~10.5gL-1Wheat bran, 11.0gL-1~12.0gL-1Corn flour,
19.0g·L-1~21.0gL-1Dregs of beans, 1.3gL-1~1.7gL-1CaCO3、2.1g·L-1~2.4gL-1MgSO4·
7H2O and 3.2gL-1~3.5gL-1NaCl, pH value 7.0~7.2.
Above-mentioned preparation method, it is preferred that the inoculum concentration of the bacillus IBFCBF-1 bacterial strains is 0.5%~1%；It is described
During liquid fermentation, temperature is 30 DEG C~35 DEG C, throughput scope 30%~100%, 180 revs/min~190 revs/min of rotating speed
Clock.
As a total technical concept, present invention also offers a kind of above-mentioned biological organic fertilizer or above-mentioned preparation method
Application of the biological organic fertilizer prepared in preventing and treating capsicum epidemic disease and plant yield-increasing.
Compared with prior art, the advantage of the invention is that：
（1）It is the Bacillus strain IBFCBF-1 containing preventing and treating capsicum epidemic disease, abundant the invention provides a kind of biological organic fertilizer
Organic matter（Weight/mass percentage composition more than 40%）, amino acid, micromolecule polypeptide and organic nutrient.Prevent and treat the gemma of capsicum epidemic disease
Bacillus strain IBFCBF-1 is the high-quality and efficient bacterial strain for suppressing Phytophthora capsici growth, its antagonistic effect highly significant.Basin
Plant result of the test and show that the control rate of capsicum epidemic disease reaches more than 75%.Organic matter, amino acid, micromolecule polypeptide and organic nutrient
It can not only promote the growth of crop Deng abundant nutriment, also be biocontrol microorganisms therein（IBFCBF-1）It is numerous there is provided growing
The condition grown, makes it be survived in soil and forms dominant population, so as to play prophylaxis effect.Therefore, biological organic fertilizer
Mechanism of action be that the beneficial microbe in this fertilizer product can enable to this beneficial micro- with organic carrier good combination
The biological rapid amount reproduction of bacterial strain, in capsicum rhizosphere formation dominant microflora；Contain in still further aspect, this fertilizer product containing higher
The organic nitrogen and organophosphor of amount, growth of these nutriments to crop in continuous cropping obstacle soil are highly beneficial, substantially increase
The vertical seedling rate and anti-adversity ability of crop.
（2）It is biological bacterial strain preparation, completely not because of the use of chemical pesticide the invention provides a kind of biological organic fertilizer
A series of problems brought, thus be conducive to the No-harmful apple orchard of vegetables, peasant can without or reduce the use of chemical pesticide
Amount, this can not only reduce expenses for peasant, and reduce the residues of pesticides in agricultural product, be conducive to the outlet of agricultural product.Together
When, the bio-feritlizer also has volume increase function, can increase income for peasant.
（3）The biological organic fertilizer of the present invention can be applied in nutritive cube, can also be applied in crop field, not only with organic fertilizer
Function, and biocontrol microorganisms contained therein are worked by a variety of growth-promotings and Biocontrol Mechanism, so as to reach growth-promoting or prevent and treat peppery
The effect of green pepper epidemic disease.
（4）The invention provides a kind of preparation method of biological organic fertilizer, preparation technology is simple and environmentally-friendly, it is extensive to be adapted to
Industrialized production.
（5）The invention provides a kind of application of biological organic fertilizer, in the serious field test of capsicum epidemic disease continuous cropping obstacle
Middle continuous administration biological organic fertilizer, can effectively control the generation of capsicum epidemic disease, while the yield of capsicum can also be improved.
Bacillus IBFCBF-1, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, protects
Tibetan address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and Institute of Microorganism, Academia Sinica, preservation date is 2015 8
The moon 11, preserving number is CGMCC NO. 11230.Bacillus IBFCBF-1 is special in application invention on December 02nd, 2015
Profit, Application No. 201510867702.4, date of publication 2016 year 03 month 02 day.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
The accompanying drawing needed to use is briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention
Example, for those of ordinary skill in the art, on the premise of not paying creative work, can also be obtained according to these accompanying drawings
Obtain other accompanying drawings.
Fig. 1 is inhibition photos of the bacillus IBFCBF-1 in the embodiment of the present invention 1 to Phytophthora capsici.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained belongs to what the present invention was protected
Scope.
Material and instrument employed in following examples are commercially available.The bacterial strain for being wherein used to prevent and treat capsicum epidemic disease is bud
Spore bacillus IBFCBF-1, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preservation address is north
The institute 3 of Jing Shi Chaoyang Districts North Star West Road 1, Institute of Microorganism, Academia Sinica, preservation date is August in 2015 11, protects
Tibetan number is CGMCC NO. 11230.
Embodiment 1
A kind of bacillus IBFCBF-1 for being used to prevent and treat capsicum epidemic disease of the invention, the health from capsicum epidemic disease morbidity field
Antagonistic Fungi is separated in pepper plant rhizosphere soil, then finally filters out by pot experiment efficient antagonistic strain IBFCBF-1.Bacterium
Plant -70 DEG C of preservations in the glycerine of volumn concentration 30%.Antagonistic Fungi IBFCBF-1 is delivered into Chinese microorganism strain preservation management
Committee's common micro-organisms center preservation, preservation date is August in 2015 11, and preserving number is CGMCC NO. 11230.
Inhibitions of the IBFCBF-1 to Phytophthora capsici is investigated, Fig. 1 left figures are control, single capsicum epidemic disease disease
Opportunistic pathogen growth figure；Right figure is that the bacterium colony in the middle of inhibitions of the IBFCBF-1 to Phytophthora capsici, right figure is pathogen, four
The bacterium colony in week is biocontrol microorganisms, it was found from Fig. 1 control：IBFCBF-1 has obvious inhibition to Phytophthora capsici.
Bacillus strain IBFCBF-1 bacterium colony is creamy white, translucent, circular, and edge is irregular, surface wettability light
Sliding, Main Biological is：Gram-positive G+, cell is shaft-like, the life of gemma end or near-end life, catalase sun
Property, the Starch Hydrolysis positive, the methyl red test positive, V-P reacting positives, gelatin liquefaction positive, the cellulose decomposition positive, nitrate
Also Antigen positive hybridomas, indole test is negative, it is impossible to utilize citrate, can produce ammonia, the sour aerogenesis of glucose fermentation production, can utilize xylose,
L-arabinose, mannose, D-Fructose, D- xyloses, optimum growth temperature are 35 DEG C, and pH value is 7.0.
Embodiment 2
A kind of biological organic fertilizer of the invention, is prepared using following methods：
1）Prepare fermentation of bacillus liquid：
1.1st, fermentation medium is prepared：Weigh 10.35 g wheat brans, 11.5 g corn flour, 20 g dregs of beans, 1.5 g
CaCO3, 2.3 g MgSO4·7H2O, 3.41 g NaCl are put into container, add water to 1000 mL, and pH value is adjusted to 7.0~
7.2, sterilize 30 min at 115 DEG C；
1.2nd, liquid fermentation：Take the bacillus IBFCBF-1 of 2 mL embodiments 1（CGMCC NO. 11230）Inoculation is arrived
Liquid fermentation production is carried out in the fermentation medium of 200mL steps 1.1, its fermentation condition is：Initial pH value scope be 7.0~
7.2, cultivation temperature is 30 DEG C, dissolved oxygen：Throughput scope 30%~100%, 190 revs/min of rotating speed ferments the middle and later periods（36 h）Shape
Into gemma, IBFCBF-1's contains bacterium or gemma amount >=10.0 × 10 in the obtained zymotic fluid that ferments9Individual/ml.
2）Prepare mixing organic fertilizer：
2.1st, sheep manure organic fertilizer is prepared：
By 82wt% fresh sheep manure, 11wt% system chaff, 3wt% sawdust, 4wt% rapeseed meal, 0.3wt% fermenting agent is mixed
Obtain composting material；By the composting material regulation water content be 55% after, beat heap and obtain heap body；The height of heap body is 1.7m, wide
Spend for 2.2m, length is not limited；The heap body is subjected to compost fermentation 15d（At room temperature）.In composting fermentation process, the heap body liter
When temperature is to 60 DEG C~65 DEG C, turning, daily turning 1~2 time are carried out；When temperature drop is to 40 DEG C~45 DEG C, every turning in two days
Once, until temperature is down to normal temperature, preserve stand-by；The sheep manure organic fertilizer of preparation, germination index is more than 98%, quality of organic matter
Than content >=40%, organic nitrogen quality is 1.7~2.5% than content, and water content is 25~30%；
2.2nd, amino acid fertilizer is prepared：Select the amino acid fertilizer of Hunan Tai Gu bio-feritlizers Co., Ltd, registration card number：
Hunan agricultural fertilizer（2016）Quasi- word 1991, fertilizer specification：N+P2O5+K2O >=6%, organic matter >=40%, amino acid >=5%；
2.3rd, mix：Sheep manure compost and amino acid fertilizer are mixed to get mixing organic fertilizer for 5: 5 by volume.
3）Solid fermentation：By step 1）Fermentation of bacillus liquid according to volume mass ratio be 20% to be inoculated into step 2）'s
Mix in organic fertilizer, ferment 7d in the environment of temperature≤55 DEG C, daily turning 1~2 time in fermentation process obtains and contains gemma
Bacillus IBFCBF-1 living bacteria counts content is 3.0 × 108Cfu/g biological organic fertilizer, moisture is evaporated to less than 30%.
The bio-organic fertilizer of the present embodiment 1, containing being not less than 3.0 × 108Individual/g above-mentioned biocontrol bacterial strain IBFCBF-
1 CFU, nitrogen（Full nitrogen）Content be 25.6 mg/kg, phosphorus（Full phosphorus）Content be 15.2 mg/kg, potassium（Entirely
Potassium）Content be 32.7 mg/kg, the % of organic matter 42.5.
Embodiment 3
A kind of biological organic fertilizer of embodiment 2 is in the application of the preventing and treating potted plant middle capsicum epidemic disease of capsicum, and concrete application method is：
The preparation of test sample：It is capsicum continuous cropping soil for examination soil, epidemic disease morbidity is serious.For examination capsicum variety " Jin Jiaohong
Dragon ".After pepper seed surface sterilization, it is seeded into nutritive cube and carries out nursery, length to 4 leaf phases produces Hot Pepper Seedling；
The potted plant preparation of capsicum：The big basin alms bowl of 90 5 kilograms of preparation, each basin alms bowl adds 5kg capsicum continuous cropping soils, and transplants 3
Hot Pepper Seedling；
Fertilising：Three parts are equally divided into by capsicum is potted plant, and every group of 30 basins carry out processing CK controls in accordance with the following methods：Applied per basin
N, P, K chemical fertilizer, using being spreaded manuer in holes before transplanting seedlings.
T1：1% is applied per basin（W/W）Step 2 in embodiment 2）Mixing organic fertilizer, using being spreaded manuer in holes before transplanting seedlings.
T2：1% is applied per basin（W/W）The biological organic fertilizer of embodiment 2, using being spreaded manuer in holes before transplanting seedlings.
The nutrient content such as N, P, K is consistent in CK controls, T1, T2 group.
Three groups of capsicums are potted plant, cultivated under identical environment, transplant 30 d samplings and calculate biomass, investigate growth-promoting effect
Really.Florescence is grown to, incidence is recorded weekly, disease index is counted, statistical result is referring to table 1.
Table 1：The potted plant incidence of disease of capsicum, biocontrol effect and biomass
| Processing | The incidence of disease | Prevention effect | Plant height（cm） | Overground part dry weight（g） |
| CK | 86.3 % | - | 22.95 ± 0.42 | 0.52 ± 0.03 |
| T1 | 37.4% | 56.6% | 24.35 ± 0.31 | 0.58 ± 0.01 |
| T2 | 17.4% | 80.1% | 27.59 ± 0.29 | 0.71 ± 0.03 |
Transplant the plant that each processing is gathered after 60 d and pedotheque and take back micro- life in lab analysis measure rhizosphere soil rapidly
Thing quantity, investigates result referring to table 2.
Table 2：Capsicum rhizosphere Soil Microorganism quantity
Result of the test is as follows：
（1）Biological organic fertilizer using the present invention can promote the growth of capsicum, dramatically increase the biomass of plant（Table 1）.At CK
The plant height of capsicum and overground part dry weight are followed successively by 42.5 cm and 3.3 g in reason, and use the biological organic fertilizer of the IBFCBF-1 containing bacterium
（T2 processing）In capsicum plant height and overground part dry weight be followed successively by 62.7 cm and 5.1 g, the capsicum biomass in T2 processing is compared
CK processing and T1 processing are intended to height；
（2）Transplant after 60 d, the incidence of disease of plant has reached 86.3 % in control treatment, and adds mixing organic fertilizer and the present invention
Middle biological organic fertilizer can significantly reduce the incidence of disease of capsicum epidemic disease, and wherein T2 shows best using biological organic fertilizer processing
Prevention effect, its incidence of disease is 17.4%, and diseases prevention rate is up to 80.1%；
（3）It can not only play a part of preventing and treating capsicum epidemic disease using the biological organic fertilizer of the present invention, and with reduction soil
The quantity of middle phytophthora blight of pepper, increases quantity of useful microbe, improves the function of rhizosphere microorganism structure of community.
Embodiment 4
A kind of biological organic fertilizer of embodiment 2 is in the application of preventing and treating field capsicum epidemic disease, and concrete application method is：
In Changsha, the capsicum epidemic disease morbidity greenhouse of Feng Jiachong area continuous croppings 8 years has carried out field test, with 100 kg biological organics
The usage amount of fertilizer/mu, sets two processing, each three repetitions of processing, and the plot area each repeated is 30 m2。
CK is compareed：Using chemical fertilizer（With n p k fertilizer polishing nutrient, the content and biological organic fertilizer for making N P and K are handled
Group is consistent）.
Biological organic fertilizer treatment group：Using biotinylated biomolecule organic fertilizer.
After capsicum transplantation of seedlings, field gathered data is periodically arrived, the incidence of disease is counted, different disposal is calculated after finally harvesting
Biomass difference.Result is investigated referring to table 3.
Table 3：Field test effect
| Processing | The incidence of disease（%） | Yield（Kg/ mus） |
| CK processing | 72.4 | 1845 |
| Biological organic fertilizer | 16.7 | 2461 |
As can be known from the results of Table 3：The incidence of disease of capsicum epidemic disease can be significantly reduced in field using the biological organic fertilizer of the present invention,
Yield of hot pepper increase by 33.4%.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (10)
1. a kind of biological organic fertilizer, it is characterised in that the biological organic fertilizer is inoculated with using bacillus IBFCBF-1 zymotic fluids
Into mixing organic fertilizer, prepared through solid fermentation；The mixing organic fertilizer includes cow dung organic fertilizer and amino acid fertilizer.
2. biological organic fertilizer according to claim 1, it is characterised in that in the biological organic fertilizer, bacillus
IBFCBF-1 viable count is 3.0 × 108More than cfu/g, the content of nitrogen is 20mg/kg~28mg/kg；The content of phosphorus is
12mg/kg~18mg/kg；The content of potassium is 28mg/kg~32mg/kg, organic matter >=40%.
3. the preparation method of biological organic fertilizer described in a kind of claim 1 or 2, it is characterised in that comprise the following steps：
S1, sheep manure organic fertilizer and amino acid fertilizer be mixed to get mixing organic fertilizer；
S2, by bacillus IBFCBF-1 zymotic fluids by volumn concentration be 15~20% be inoculated into mixing organic fertilizer consolidated
Body fermentation obtains biological organic fertilizer.
4. preparation method according to claim 3, it is characterised in that in the S1 steps, the sheep manure organic fertilizer and ammonia
The volume ratio of base acid organic fertilizer is 5: 5；And/or, in the S2 steps, in the solid fermentation process：In temperature≤55 DEG C
Ferment 7~8d under environment, and turning daily obtains the biological organic fertilizer of the IBFCBF-1 containing bacillus for 1~2 time in fermentation process, its
Water content is below 30%.
5. preparation method according to claim 3, it is characterised in that the preparation method of the sheep manure organic fertilizer is：
S1-1, the fresh sheep manure by 80wt%~85wt%, 10wt%~12wt% system chaff, 2wt%~5wt% sawdust, 3wt%~
5wt% rapeseed meal, 0.3wt% fermenting agent is uniformly mixed so as to obtain composting material；
S1-2, by the composting material regulation water content be 50%~60% after, beat heap and obtain heap body；
S1-3, the heap body is subjected to compost fermentation 12d~18d.
6. preparation method according to claim 5, it is characterised in that in the S1-2 steps, the height of the heap body is
1.5m~2m, width is 2.0m~2.5m；And/or, in the S1-3 steps, when the heap body is warming up to 60 DEG C~65 DEG C,
Carry out turning, daily turning 1~2 time；When temperature drop is to 40 DEG C~45 DEG C, every turning in two days once, until temperature is down to
Normal temperature, is preserved stand-by.
7. preparation method according to claim 3, it is characterised in that 10% >=N+P in the amino acid fertilizer2O5+K2O
>=6%, organic matter >=40%, 8% >=amino acid >=5%.
8. preparation method according to claim 3, it is characterised in that in the S2 steps, the bacillus IBFCBF-
1 zymotic fluid is prepared using following methods：Bacillus IBFCBF-1 inoculations are subjected to liquid into fermentation medium
Fermentation, forms gemma；The liquid fermentation medium includes 10.1gL-1~10.5gL-1Wheat bran, 11.0gL-1~
12.0g·L-1Corn flour, 19.0gL-1~21.0gL-1Dregs of beans, 1.3gL-1~1.7gL-1CaCO3、2.1g·L-1~
2.4g·L-1MgSO4·7H2O and 3.2gL-1~3.5gL-1NaCl, pH value 7.0~7.2.
9. preparation method according to claim 7, it is characterised in that the inoculum concentration of the bacillus IBFCBF-1 bacterial strains
For 0.5%~1%；During the liquid fermentation, temperature is 30 DEG C~35 DEG C, throughput scope 30%~100%, 180 turns of rotating speed/
Minute~190 revs/min.
10. prepared any one of the biological organic fertilizer or claim 3 to 9 any one of a kind of claim 1 to 2
Application of the biological organic fertilizer that method is prepared in preventing and treating capsicum epidemic disease and plant yield-increasing.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN111606762A (en) * | 2020-05-14 | 2020-09-01 | 标优美生态工程股份有限公司 | Rapid composting microbial inoculum and method for producing organic fertilizer by using same |
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| CN109928838A (en) * | 2019-05-06 | 2019-06-25 | 湖南泰谷生态工程有限公司 | Rosemary organic-inorganic compound fertilizer special and the preparation method and application thereof |
| CN111606762A (en) * | 2020-05-14 | 2020-09-01 | 标优美生态工程股份有限公司 | Rapid composting microbial inoculum and method for producing organic fertilizer by using same |
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