CN107173555A - The optimization processing of pomegranate seed in a kind of sheep feed - Google Patents
The optimization processing of pomegranate seed in a kind of sheep feed Download PDFInfo
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- CN107173555A CN107173555A CN201710307365.2A CN201710307365A CN107173555A CN 107173555 A CN107173555 A CN 107173555A CN 201710307365 A CN201710307365 A CN 201710307365A CN 107173555 A CN107173555 A CN 107173555A
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- Prior art keywords
- pomegranate seed
- pomegranate
- seed oil
- seed
- granada
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
- A23K40/35—Making capsules specially adapted for ruminants
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
- Birds (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of optimization processing of pomegranate seed in sheep feed, pomegranate seed is passed through into ultramicro grinding first, it is again the microcapsules that cyst material prepares granada seed oil to yeast cells, again pomegranate seed albumen is extracted with alkali extraction-acid precipitation, after being separated, pomegranate seed slag is used into candida utili, aspergillus niger hybrid bacterial strain fermenting and preparing biological feed again, the nutritive value of pomegranate seed is taken full advantage of, wherein emphasis application embedding techniques of the present invention overcomes granada seed oil oxidative rancidity problem in storage.The value of pomegranate seed is fully excavated, so as to effectively facilitate growing for cultivated animals.
Description
Technical field
The present invention relates to a kind of optimization processing of pomegranate seed in field of feed, more particularly to sheep feed.
Background technology
The oil content of pomegranate seed is about 40-50%, and conjugated triene is contained in its structure, and physiologically active is higher, be it is a kind of very
Excellent antioxidant.Granada seed oil can be also used for food additives, to increase flavour of food products, improve its healthcare function.Stone
Pomegranate seed oil and extract have resistance inflammation and oxygen radical to the damage of human body, anti-aging, prevention of arterial atherosis,
Prevent and treat the effect such as breast cancer, hypoglycemic, anti diar rhea.
But, the problem of presently, there are is containing abundant unrighted acid in granada seed oil, therefore in processing, storage
Occurred Auto-oxidation reaction by the effect of air, illumination, enzyme and metal ion etc. with easy during use, cause change of becoming sour
Matter.So, how to prevent that granada seed oil oxidative rancidity in food processing and storage from being one the problem of can not be ignored.
Instant invention overcomes above mentioned problem, using the technique microencapsulation granada seed oil, it would be desirable to protect granada seed oil embedding
Be solidificated in cyst wall, so as to protect it from the influence of external environment condition, reduce volatility, improve stability.The present invention is with yeast
Cell is the features such as cyst material prepares nontoxic microcapsules, good biocompatibility, easily biological-degradable.
The content of the invention
The object of the invention be exactly in order to make up the defect of prior art there is provided a kind of optimization of pomegranate seed in sheep feed at
Reason.
The present invention is achieved by the following technical solutions：
A kind of optimization processing of pomegranate seed in sheep feed, preparation method is comprised the following steps that：(1) that pomegranate seed is placed in into vacuum is cold
Freeze in drying box and dry, first pass through coarse crushing, it is finely divided, then carry out ultramicro grinding；(2) above-mentioned ultra micro pomegranate powder is added into solvent
Ethanol-petroleum ether mixed extract than 1: 2, is extracted 2-3 times, separation slag charge and leaching liquor, then leaching liquor is concentrated under reduced pressure into
Solvent is evaporated, and temperature control obtains granada seed oil at 80-90 DEG C；(3) water that the slag charge in step 2 is added into 10-12 times crosses glue
Body is ground, the tune pH value 8.0-8.4 of NaOH solution under conditions of 45-50 DEG C of feed temperature；Slurry centrifugation, supernatant are carried out afterwards
Add hydrochloric acid, the heavy process pH value of acid is adjusted under the conditions of 4.0-4.3,3500-3700r/min after centrifugal treating again by pH values tune
To 6.8-7.2, spray drying obtains pomegranate seed albumen powder；(4) cooled down after dry ferment is inactivated through 105-110 DEG C, by 1:8 ratio
Example adds water scattered, adds the granada seed oil of step 2, with high-speed shearing disperser in 8000-8200r/min emulsifying homogeneous 6-8 seconds,
It is put into thermostatic control oscillator vibration, after constant temperature constant speed vibration embedding under the conditions of 100-120 r/min, adds isometric 80-
85% ethanol solution, stirs, and stands 30-35 minute, removing supernatant, will be deposited under the conditions of 600-640 r/min from
The heart is separated 10-13 minutes, and centrifugal sediment cleans the granada seed oil of remained on surface, 60-65 through vacuum filtration and with absolute ethyl alcohol
DEG C vacuum drying 24-26 hours, obtain granada seed oil microcapsules；(5) step 3 is isolated into obtain slag charge and wheat bran, urea, grape
Distilled water is added after sugar mixing and adjusts humidity to 65-70%, 121-125 DEG C sterilizes 20-25 minutes, then by candida utili, black
Aspergillus presses 1:1 ratio adds above-mentioned material and is well mixed, and is placed in 37-42 DEG C and ferments 7-8 days, with step 3 after drying powder processed
Pomegranate seed albumen powder, step 4 granada seed oil microcapsules be well mixed.
Preferably, the condition of vacuum refrigeration is that temperature is subzero 35-30 DEG C in pomegranate seed in the step 1, and pressure is
20-25Pa。
Preferably, the condition extracted in the step 2 is the solid-liquid ratio 1: 8 of pomegranate seed Ultramicro-powder and solvent, extraction temperature
90-95 DEG C, extraction time 60-70 minutes.
Preferably, alkali, the concentration of acid solution are respectively 0.15-0.17mol/L, 10-12mol/L in the step 3.
Preferably, it is 1 that granada seed oil disperses liquid proportional with dry ferment in the step 4:1, embedding temperature be 70-74 DEG C,
Embedding 4-5 hours.
Preferably, pomegranate seed, wheat bran, urea, glucose quality ratio are 16 in the step 5:2:1:1.
Preferably, candida utili, aspergillus niger inoculum concentration are 5% in the step 5.
It is an advantage of the invention that：Pomegranate seed passes through ultramicro grinding, and part cell membrane is destroyed, and is conducive to granada seed oil
Leach.Yeasts non-viable is more stable than living cells property, and part increases its cell membrane and membrane passage, using at a high speed
Water, inactivation dry ferment and granada seed oil can be emulsified into homogeneous system under certain condition by disperser, so as to be conducive to pomegranate seed
Oil is penetrated into the inactive yeast cell being water-swellable with diffusion in the form of oily emulsion droplet.Candida utili, aspergillus niger hybrid bacterial strain
The advantage of fermentation is, fermentation time can be reduced first, and tunning has yeast fragrance, effectively solves the bottom in fermentation process
Thing suppression, reduces content of cellulose, improves animal use rate, is the fermentation method of efficient energy-saving.Secondly, mixed fermentation side
Formula is easy to operate, can effectively improve thick protein and polyphenol content, can reduce feed cost effectively as feed additive,
The crude protein improved in salvage value, tunning can provide the polyphenol in the proteinogen of high-quality, product for cultivated animals
Material has oxidation resistance, effectively facilitates growing for cultivated animals.
Embodiment
A kind of optimization processing of pomegranate seed in sheep feed, preparation method is comprised the following steps that：(1) pomegranate seed is placed in very
Dried in vacuum freecing-dry case, first pass through coarse crushing, it is finely divided, then carry out ultramicro grinding；(2) above-mentioned ultra micro pomegranate powder is added
Ethanol of the solvent than 1: 2-petroleum ether mixed extract, is extracted 2 times, separation slag charge and leaching liquor, then leaching liquor is concentrated under reduced pressure
It is evaporated to solvent, temperature control obtains granada seed oil at 80 DEG C；(3) water that the slag charge in step 2 is added into 10 times crosses colloid
The tune pH value 8.0 of NaOH solution under conditions of mill, 45 DEG C of feed temperature；Slurry centrifugation is carried out afterwards, and supernatant adds hydrochloric acid,
The heavy process pH value of acid is adjusted to that pH values are adjusted into 7.0 again after centrifugal treating under the conditions of 4.0,3500r/min, is spray-dried, obtains stone
Pomegranate seed albumen powder；(4) cooled down after dry ferment is inactivated through 105 DEG C, by 1:8 ratio add water it is scattered, add step 2 pomegranate seed
Oil, with high-speed shearing disperser in 8000r/min emulsifying homogeneous 6 seconds, is put into thermostatic control oscillator vibration, in 100 r/min bars
Under part after constant temperature constant speed vibration embedding, 80% isometric ethanol solution is added, is stirred, 30 minutes are stood, supernatant is removed
Liquid, will be deposited under the conditions of 600r/min and centrifuges 10 minutes, and centrifugal sediment is cleaned through vacuum filtration and with absolute ethyl alcohol
The granada seed oil of remained on surface, 60 DEG C are dried in vacuo 24 hours, obtain granada seed oil microcapsules；(5) step 3 is isolated into obtain slag
Material mix with wheat bran, urea, glucose after add distilled water adjust humidity to 65%, 121 DEG C sterilize 20 minutes, then will produce protein vacation
Silk yeast, aspergillus niger press 1:1 ratio adds above-mentioned material and is well mixed, and is placed in 37 DEG C and ferments 7 days, dry after powder processed and
The pomegranate seed albumen powder of step 3, the granada seed oil microcapsules of step 4 are well mixed.
Preferably, the condition of vacuum refrigeration is that temperature is subzero 35 DEG C in pomegranate seed in the step 1, and pressure is 20Pa.
Preferably, the condition extracted in the step 2 is the solid-liquid ratio 1: 8 of pomegranate seed Ultramicro-powder and solvent, extraction temperature
90 DEG C, extraction time 60 minutes.
Preferably, alkali, the concentration of acid solution are respectively 0.15mol/L, 10mol/L in the step 3.
Preferably, it is 1 that granada seed oil disperses liquid proportional with dry ferment in the step 4:1, embedding temperature is 70 DEG C, embedding
4 hours.
Preferably, pomegranate seed, wheat bran, urea, glucose quality ratio are 16 in the step 5:2:1:1.
Preferably, candida utili, aspergillus niger inoculum concentration are 5% in the step 5.
Claims (7)
1. the optimization processing of pomegranate seed in a kind of sheep feed, it is characterised in that preparation method is comprised the following steps that：(1) by stone
Pomegranate seed is placed in vacuum freezing drying oven and dried, and first passes through coarse crushing, finely divided, then carries out ultramicro grinding；(2) by above-mentioned ultra micro
Pomegranate powder adds ethanol of the solvent than 1: 2-petroleum ether mixed extract, extracts 2-3 times, separation slag charge and leaching liquor, then will leaching
Extract is concentrated under reduced pressure into solvent and is evaporated, and temperature control obtains granada seed oil at 80-90 DEG C；(3) slag charge in step 2 is added
10-12 times of water crosses the tune pH value 8.0-8.4 of NaOH solution under conditions of colloid mill, 45-50 DEG C of feed temperature；After carry out slurry
Centrifuge, supernatant adds hydrochloric acid, the heavy process pH value of acid is adjusted under the conditions of 4.0-4.3,3500-3700r/min at centrifugation
PH values are adjusted to 6.8-7.2 again after reason, is spray-dried, obtains pomegranate seed albumen powder；(4) after dry ferment is inactivated through 105-110 DEG C
Cooling, by 1:8 ratio add water it is scattered, add step 2 granada seed oil, with high-speed shearing disperser in 8000-8200r/min
Emulsifying homogeneous 6-8 seconds, is put into thermostatic control oscillator vibration, after constant temperature constant speed vibration embedding under the conditions of 100-120 r/min, plus
Enter isometric 80-85% ethanol solution, stir, stand 30-35 minutes, remove supernatant, 600-640 will be deposited in
Centrifuged 10-13 minutes under the conditions of r/min, centrifugal sediment cleans the stone of remained on surface through vacuum filtration and with absolute ethyl alcohol
Pomegranate seed oil, 60-65 DEG C is dried in vacuo 24-26 hours, obtains granada seed oil microcapsules；(5) step 3 is isolated into obtain slag charge and bran
Distilled water is added after skin, urea, glucose mixing and adjusts humidity to 65-70%, 121-125 DEG C sterilizes 20-25 minutes, then will production
Protein Candida, aspergillus niger press 1:1 ratio adds above-mentioned material and is well mixed, and is placed in 37-42 DEG C and ferments 7-8 days, drying
It is well mixed after powder processed with the pomegranate seed albumen powder of step 3, the granada seed oil microcapsules of step 4.
2. the method according to claims 1, it is characterised in that in the step 1 in pomegranate seed vacuum refrigeration condition
It is that temperature is subzero 35-30 DEG C, pressure is 20-25Pa.
3. the method according to claims 1, it is characterised in that：The condition extracted in the step 2 is pomegranate seed ultra micro
The solid-liquid ratio 1: 8 of powder and solvent, 90-95 DEG C of extraction temperature, extraction time 60-70 minutes.
4. the method according to claims 1, it is characterised in that：Alkali, the concentration of acid solution are respectively in the step 3
0.15-0.17mol/L、10-12mol/L。
5. the method according to claims 1, it is characterised in that：Granada seed oil and dry ferment dispersion liquid in the step 4
Ratio is 1:1, embedding temperature is 70-74 DEG C, embedded 4-5 hours.
6. the method according to claims 1, it is characterised in that：Pomegranate seed, wheat bran, urea, glucose in the step 5
Mass ratio is 16:2:1:1.
7. the method according to claims 1, it is characterised in that：Candida utili, aspergillus niger inoculation in the step 5
Measure as 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710307365.2A CN107173555A (en) | 2017-05-04 | 2017-05-04 | The optimization processing of pomegranate seed in a kind of sheep feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710307365.2A CN107173555A (en) | 2017-05-04 | 2017-05-04 | The optimization processing of pomegranate seed in a kind of sheep feed |
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| Publication Number | Publication Date |
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| CN107173555A true CN107173555A (en) | 2017-09-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710307365.2A Pending CN107173555A (en) | 2017-05-04 | 2017-05-04 | The optimization processing of pomegranate seed in a kind of sheep feed |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785218A (en) * | 2005-11-22 | 2006-06-14 | 华中科技大学 | Extractive of pomegranate seeds, prepn. method and application in preparing medicine thereof |
| CN102936276A (en) * | 2012-11-02 | 2013-02-20 | 石河子大学 | Method for extracting pomegranate seed protein |
| CN103710147A (en) * | 2013-12-26 | 2014-04-09 | 江南大学 | Preparation method of pomegranate seed oil pocket |
-
2017
- 2017-05-04 CN CN201710307365.2A patent/CN107173555A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785218A (en) * | 2005-11-22 | 2006-06-14 | 华中科技大学 | Extractive of pomegranate seeds, prepn. method and application in preparing medicine thereof |
| CN102936276A (en) * | 2012-11-02 | 2013-02-20 | 石河子大学 | Method for extracting pomegranate seed protein |
| CN103710147A (en) * | 2013-12-26 | 2014-04-09 | 江南大学 | Preparation method of pomegranate seed oil pocket |
Non-Patent Citations (1)
| Title |
|---|
| EMAMI A等: "饲料中添加石榴籽浆对幼龄山羊肌肉组织和脂肪组织中共轭亚油酸和亚麻酸含量的影响", 《饲料博览》 * |
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Application publication date: 20170919 |