A kind of livestock and poultry cultivation physical efficiency modifying agent and preparation method and applications
Technical field
The present invention relates to livestock and poultry cultivation field, and in particular to a kind of livestock and poultry cultivation physical efficiency modifying agent and preparation method and its should
With.
Background technology
For animal in clinical onset, what our traditional idea considered is how virus to be killed, how bacterium killed,
Killing simply, it is last the result is that whatCause the Antibiotic Resistance of cause of disease more and more wider, so cause in the future we clinically permitted
More diseases past medical help, and in face of the phenomenon to past medical help, the disease in future is necessarily increasingly difficult to control, it might even be possible to says it is nowhere
Set about, so random dispensing simply, may finally jeopardize our human healths.
Cause of disease in animal and external environment in fact, can reach harmonious coexistence in a way.Defence for body
For barrier, we consider mucous membrane first, then skin, then after pathogenic microorganism breaches these barriers, liver
Body can be effectively assisted to resist the last of extraneous pathogenic microorganism invasion by could also say that as its second defence line
One of barrier!Especially birds, birds do not have lymph node, these cause of diseases will finally enter liver, once liver is bad, and it is external each
Kind antigen or some poisonous and hazardous materials will be directly entered blood, enter in the whole blood circulation of whole body, it will
Exert an adverse impact to overall each tract;For body under pathological state, animal body will transfer body itself
The reserve of various aspects.First, that repairs its own resists barrier, secondly protecting supply, this protects supply that is in body,
Each material composition in environment in body fluid, under pathological state, remains able to meet that each organ, the histocyte of body are normal
The completion of physiological activity, the work of this part are mostly to be completed by liver, therefore, protect the liver of livestock and poultry extremely heavy
Will.
The content of the invention
The defects of present invention is directed to above in the prior art provides a kind of livestock and poultry cultivation physical efficiency modifying agent, livestock and poultry improvement
Agent can protect the liver of livestock and poultry to be encroached on from toxicant.
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, including 80-100 parts of choline chloride, D-sorbite 8-10
Part, 15-20 parts of inositol, 15-20 parts of methionine, 10-15 parts of lysine, purified water is 830-880 parts.
Further, the content of choline chloride is 60~70mg/ml in the livestock and poultry cultivation physical efficiency modifying agent.
Further, vitamin B1 is further included, the weight content of the vitamin B1 is various composition gross weight
0.012%-0.02%.
Further, the livestock and poultry cultivation physical efficiency modifying agent is yellow to brownish red supernatant liquid.
Further, in parts by weight, including 80 parts of choline chloride, 8 parts of D-sorbite, 15 parts of inositol, methionine 15
Part, 10 parts of lysine, purified water is 830 parts, and the weight of vitamin B1 is the 0.012% of mentioned component gross weight.
Further, in parts by weight, including 90 parts of choline chloride, 9 parts of D-sorbite, 18 parts of inositol, methionine 18
Part, 12 parts of lysine, purified water is 850 parts, and the weight of vitamin B1 is the 0.015% of mentioned component gross weight.
Further, in parts by weight, including 100 parts of choline chloride, 10 parts of D-sorbite, 20 parts of inositol, methionine
20 parts, 15 parts of lysine, purified water is 880 parts, and the weight of vitamin B1 is the 0.02% of mentioned component gross weight.
Present invention also offers the preparation method of above-mentioned livestock and poultry cultivation physical efficiency modifying agent, include the following steps:
Choline chloride, D-sorbite, inositol, methionine, lysine, purified water and Wei Sheng are weighed respectively according to parts by weight
Plain B1, is stirred for being completely dissolved up to livestock and poultry cultivation physical efficiency modifying agent.
Present invention also offers above-mentioned livestock and poultry cultivation physical efficiency modifying agent to prepare the medicine for treating, improving livestock and poultry liver function
Application in thing.
Further, when preparing for treating, improving the medicine of livestock and poultry liver function, the dosage of the modifying agent
For:
The modifying agent 500ml is taken to be watered 500kg, 24 continuously drink when small, are used in conjunction 3-5 days.
Further, the temperature of the water is no more than 40 degree.
Compared with prior art, the present invention has at least the following advantages:
1. livestock and poultry cultivation physical efficiency modifying agent provided by the invention can protect the liver of livestock and poultry from the infringement of toxicant.
2. livestock and poultry cultivation physical efficiency modifying agent provided by the invention has the anti-oxidation function of strength, the liver of livestock and poultry can be protected
The dirty destruction from free radical.
3. livestock and poultry cultivation physical efficiency modifying agent provided by the invention can promote the synthesis of protein, make liver in damaged condition thin
The quick self-healing of born of the same parents.
4. livestock and poultry cultivation physical efficiency modifying agent provided by the invention can accelerate the generation of new liver cell, can expire
Each organ of sufficient body, the demand of histocyte normal physiological activity.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, below to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that described embodiment is part of the embodiment of the present invention, rather than whole realities
Apply example.Therefore, the scope of claimed invention is not intended to limit to the detailed description of the embodiment of the present invention below, and
It is the selected embodiment for being merely representative of the present invention.Based on the embodiments of the present invention, those of ordinary skill in the art are not making
Go out all other embodiments obtained under the premise of creative work, belong to the scope of protection of the invention.
Below by specific embodiment, the present invention is further illustrated.
In the formula of the present invention, the characteristics of each component and function is as follows:
Choline chloride is used to treat fatty liver and hepatic sclerosis.Poultry and livestock feed additive is also served as, ovary can be stimulated to lay eggs, more
The weightening such as farrowing and poultry, fish.
Inositol plays the role of fat metabolism and cholesterol, reduces cholesterol, helps prevention of arterial hardening.Liver is removed in help
Fat.
Methionine can be used for the liver diseases such as prevention chronic or acute hepatitis, hepatic sclerosis, it can also be used to alleviate arsenic, three chloromethanes
The toxic reaction of the harmful substances such as alkane, carbon tetrachloride, benzene, pyridine and quinoline.
Lysine has the function that the normal growth and development of maintenance animal and the normal function of nervous system important.Feed
Middle shortage lysine can cause negative nitrogen effect, i.e., breaks down proteins and be discharged, protein in feed from making full use of, is made
It is slow into growth of animal, it there is also the symptoms such as fatty liver, the reduction of sclerotin calcification rate.
Vitamin B1 is transformed into b1thiaminpyrophosphate (also known as cocarboxylase) in vivo, participates in the metabolism of sugar in vivo.Cause
During this Vitamin B1 deficiency, the oxidation of sugar within the organization is affected.It also has the effect for suppressing cholinesterase activity, lacks dimension
This enzymatic activity is excessive during raw element B1, and acetylcholine (one of neurotransmitter) considerable damage makes nerve conduction be affected, and can cause
Gastrointestinal peristalsis is slow, and digestive secretion is reduced, the obstacle such as loss of appetite, indigestion.
D-sorbite is as stabilizer.
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, including 80-100 parts of choline chloride, D-sorbite 8-10
Part, 15-20 parts of inositol, 15-20 parts of methionine, 10-15 parts of lysine, purified water is 830-880 parts.Wherein, choline chloride
Content is 60~70mg/ml, and livestock and poultry cultivation physical efficiency modifying agent is yellow to brownish red supernatant liquid.
Vitamin B1 is further included in preferred embodiment provided by the invention, the weight content of vitamin B1 is various
The 0.012%-0.02% of ingredient weight.
The preparation method of livestock and poultry cultivation physical efficiency modifying agent provided by the invention, includes the following steps:
Choline chloride, D-sorbite, inositol, methionine, lysine, purified water and Wei Sheng are weighed respectively according to parts by weight
Plain B1, is stirred for being completely dissolved up to livestock and poultry cultivation physical efficiency modifying agent.
Each embodiment that embodiment is seen below.
Embodiment 1
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 1.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in 1 embodiment 1 of table
Component |
Content |
Choline chloride |
80 parts |
D-sorbite |
8 parts |
Inositol |
15 parts |
Methionine |
15 parts |
Lysine |
10 parts |
Purified water |
830 parts |
Further include 0.012% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method, includes the following steps:
Choline chloride, D-sorbite, inositol, methionine, lysine, purified water and Wei Sheng are weighed respectively according to parts by weight
Plain B1, is stirred for being completely dissolved up to livestock and poultry cultivation physical efficiency modifying agent.
Embodiment 2
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 2.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in 2 embodiment 2 of table
Component |
Content |
Choline chloride |
90 parts |
D-sorbite |
9 parts |
Inositol |
18 parts |
Methionine |
18 parts |
Lysine |
12 parts |
Purified water |
850 parts |
Further include 0.015% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method is the same as embodiment 1.
Embodiment 3
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 3.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in 3 embodiment 3 of table
Component |
Content |
Choline chloride |
100 parts |
D-sorbite |
10 parts |
Inositol |
20 parts |
Methionine |
20 parts |
Lysine |
15 parts |
Purified water |
880 parts |
Further include 0.02% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method is the same as embodiment 1.
Embodiment 4
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 4.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in 4 embodiment 4 of table
Component |
Content |
Choline chloride |
85 parts |
D-sorbite |
10 parts |
Inositol |
19 parts |
Methionine |
16 parts |
Lysine |
13 parts |
Purified water |
870 parts |
Further include 0.012% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method is the same as embodiment 1.
The measure of 5 livestock and poultry cultivation physical efficiency modifying agent choline chloride of embodiment
1) instrument used in experimentation, equipment include:
Constant Temp. Oven:Controllable temperature is in (105 ± 2) DEG C
Electronic balance:Scale division value is 0.0001g
Glass sand core crucible:Filter plate aperture (4~7) um.
2) preparation of reagents
Hydrochloric acid solution:Concentrated hydrochloric acid is with purified water according to V:V=1:4 prepare.
Sodium hydroxide solution:400g/L.
Reinecke's salt (diaminotetrathiocyanodiammonochromate) methanol solution:20g/L;2g Reinecke's salts are weighed, are dissolved in 100mL first
Alcohol, filtering;The solution is made into after 48h and cannot use.
Methyl red-methylene blue mixing indicator solution.
3) analytical procedure
The livestock and poultry cultivation physical efficiency modifying agent 1g prepared by above-described embodiment 1-4 is weighed, 0.0002g is accurate to, is placed in 100ml
In beaker in tall form, add 1 drop methyl red, one methylene blue mixing indicator solution, aubergine (pH5~pH6) is adjusted to hydrochloric acid solution, in
Less than 5 DEG C are cooled in ice-water bath, is continually maintained in ice-water bath, under agitation, 15ml Reinecke's salt methanol solutions are slowly added dropwise
Liquid, continues stirring reaction 30min;Still aging 30min again.Precipitation is transferred to and has been dried at (105 scholar 2) DEG C to matter in advance
Measure in constant glass sand core mandarin orange misfortune, decompression filters, precipitation is washed with water 3~4 times, every time with water about 10ml.Precipitation will be housed
Glass sand core crucible move into (105 scholar 2) DEG C electrically heated drying cabinet, dry 2h, takes out.In being cooled to room temperature in drier, claim
Amount.
While measure, by the step identical with measure, done to being not added with sample using the reagent solution of identical quantity
Blank test.
4) interpretation of result
The content X for representing choline chloride with mass fraction is calculated by formula (1):
In formula, W1--- -- precipitates the numerical value of quality, and unit is gram (g);
W2--- -- blank test precipitates the numerical value of quality, and unit is gram (g);
The numerical value of W----- sample quality, unit are gram (g).
The arithmetic mean of instantaneous value for taking parallel determinations is measurement result, and the absolute of parallel determinations is not more than deviation
0.5%.
According to the above method measure livestock and poultry cultivation physical efficiency modifying agent in embodiment 1-4 choline chloride be respectively 56%,
63.2%th, 69.4% and 59.2%.
Hepatic function improvement effect of the 6 livestock and poultry cultivation physical efficiency modifying agent of embodiment to chronic hepatitis model mice
1) the ICR mouse 30 of about 30g are chosen, wherein 20,3 times a week to intraperitoneal administration carbon tetrachloride (V/V=
10% corn oil), first dosage is 330 μ l/kg, and second is 400 μ l/kg, is later 500 μ l/kg, is administered 6 weeks;
Other 10 normal feed is not administered and is used as normal group.
2) after 6 weeks, 20 mouse of administration are equally divided into two groups of A, B, every group is 10.Wherein A groups are model group;B
Group is administration group.The continuous intraperitoneal administration of mouse in B groups 5 days, is the livestock and poultry cultivation physical efficiency modifying agent of 1ml/kg every time.Wherein,
Prepared livestock and poultry cultivation physical efficiency modifying agent in livestock and poultry cultivation physical efficiency modifying agent selection example 3.The continuous abdominal cavity of mouse in A groups
Physiological saline 1ml/kg, successive administration 5 days is administered.
3) after being administered, from big venous blood collection, 4000rpm centrifugation 15min, collect supernatant as plasma sample.
4) measure the AST (aspartate transaminase, GOT) in plasma sample and ALT (alanine aminotransferase, GPT) contains
Amount.Experimental result is as shown in table 5 below.
The content of AST and ALT in 5 each group mice plasma sample of table
|
Normal group |
Model group |
Administration group |
AST |
36 |
193 |
41 |
ALT |
20 |
89 |
26 |
The data obtained is the average value of the AST and ALT numerical value measured by all mouse in each group in table 5.AST and
ALT is the important parameter for showing liver cell obstacle, as can be seen from Table 5, the livestock and poultry cultivation body prepared by the embodiment of the present invention 3
Energy modifying agent can effectively treat dysfunction of liver, improve liver function, and impaired liver cell can be made to return to normal level.
The livestock and poultry cultivation physical efficiency modifying agent that experiment other embodiments of the invention provide has similar result.
7 livestock and poultry cultivation physical efficiency modifying agent of embodiment is being prepared for the application in treating, improving the medicine of livestock and poultry liver function
When preparing for treating, improving the medicine of livestock and poultry liver function, the dosage of the modifying agent is:Take above-mentioned reality
The modifying agent 500ml for applying the preparation in a 1-4 is watered 500kg, and 24 continuously drink when small, are used in conjunction 3-5 days.
By the livestock and poultry cultivation physical efficiency improved application that the embodiment of the present invention 3 provides in certain pig farm of Jinan City.After, Ji
Weight gain of the pig on certain pig farm of Nanshi substantially than the pig on other pig farms is fast, wherein, control group uses water spray as control, experiment
Group sprays the livestock and poultry cultivation physical efficiency modifying agent prepared by embodiment 3, and Follow-up observation 1 year, carries out test pig daily observation and inspection
Survey, experimental result is as follows:
Compared with control group, experimental group porcine respiratory disease incidence is decreased obviously, and incidence is 18% in control group, and
The incidence of experimental group is less than 1%, and in experimental group pig adding weight apparently higher than control group.
The livestock and poultry cultivation physical efficiency modifying agent that other embodiments of the invention provide also has similar experimental result.
7 livestock and poultry cultivation physical efficiency modifying agent of embodiment is preparing the application in being used to treat the medicine of livestock and birds respiratory disease
When preparing the medicine for treating porcine respiratory disease, the dosage of the modifying agent is:Take the improvement
Agent 100ml is watered 500 kilograms, and 24 continuously drink when small, are used in conjunction 3-4 days.
The livestock and poultry cultivation physical efficiency modifying agent that the embodiment of the present invention 3 is provided is applied to Jinan City pig farm, chooses this and supports
A porkling totally 58 is grown, its size, weight are differed within 3%, its stochastic averagina are divided into 2 groups, one group is control group, and one group is
Experimental group.Wherein, the pig in experimental group, drinks livestock and poultry cultivation physical efficiency modifying agent prepared by embodiment 3 every month once;Control
Group normal water.Observe and record the upgrowth situation of pig, weigh after 7 months, wherein the weight gain of the pig in experimental group
The average value of amount is 1.34 times in control group, is found during raising, the feed intake of the porkling in experimental group substantially compares
It is big in control group, and the porkling in experimental group is substantially without the situation of falling ill.
The livestock and poultry cultivation physical efficiency modifying agent that other embodiments of the invention provide also has similar experimental result.
Livestock and poultry cultivation physical efficiency modifying agent provided by the invention can protect the liver of livestock and poultry from the infringement of toxicant, energy
Enough accelerate the production of new liver cell, can meet each organ of body, histiocytic normal physiological activity need
Ask so that porkling reduces rate of falling ill, and improves disease resistance.
More than, it is merely preferred embodiments of the present invention, but the protection domain invented is not limited thereto, and it is any ripe
Know those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be subject to the protection domain of claims.