A kind of livestock and poultry cultivation physical efficiency modifying agent and preparation method and applications
Technical field
The present invention relates to livestock and poultry cultivation field, and in particular to a kind of livestock and poultry cultivation physical efficiency modifying agent and preparation method and its should
With.
Background technology
Animal is in clinical onset, and what our traditional idea considered is how virus to be killed, how bacterium is killed,
Killing simply, what last result isCause the Antibiotic Resistance of cause of disease more and more wider, so cause in the future we clinically permitted
Many diseases past medical help, in face of the phenomenon pasted medical help, and the disease in future is necessarily increasingly difficult to control, it might even be possible to say it is nowhere
Set about, so random dispensing simply, may finally jeopardize our human healths.
The cause of disease in animal and external environment, can reach harmonious coexistence in a way in fact.For the defence of body
For barrier, we consider mucous membrane first, then skin, then after pathogenic microorganism breaches these barriers, liver
Second defence line as it, which could also say that, can effectively assist body to resist the last of extraneous pathogenic microorganism invasion
One of barrier!Especially birds, birds do not have lymph node, and these cause of diseases will finally enter liver, once liver is bad, and it is external each
Blood will be directly entered by planting antigen or some poisonous and hazardous materials, in the whole blood circulation for entering whole body, it will
Each overall tract is exerted an adverse impact;Body is under pathological state, and animal body will transfer body itself
The reserve of various aspects.First, repair its own and resist barrier, next to that protect supply, this protects supply that is in body,
The material composition of each in environment in body fluid, under pathological state, each organ, the histocyte for remaining able to meet body are normal
The completion of physiological activity, this part work is mostly what is completed by liver, therefore, protects the liver of livestock and poultry extremely to weigh
Will.
The content of the invention
Present invention defect of the prior art for more than provides a kind of livestock and poultry cultivation physical efficiency modifying agent, livestock and poultry improvement
Agent can protect the liver of livestock and poultry to be encroached on from toxicant.
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, including 80-100 parts of Choline Chloride, D-sorbite 8-10
Part, 15-20 parts of inositol, 15-20 parts of methionine, 10-15 parts of lysine, purified water is 830-880 parts.
Further, the content of Choline Chloride is 60~70mg/ml in the livestock and poultry cultivation physical efficiency modifying agent.
Further, in addition to vitamin B1, the weight content of the vitamin B1 is various composition gross weight
0.012%-0.02%.
Further, the livestock and poultry cultivation physical efficiency modifying agent is yellow to brownish red supernatant liquid.
Further, in parts by weight, including 80 parts of Choline Chloride, 8 parts of D-sorbite, 15 parts of inositol, methionine 15
Part, 10 parts of lysine, purified water is 830 parts, and the weight of vitamin B1 is the 0.012% of mentioned component gross weight.
Further, in parts by weight, including 90 parts of Choline Chloride, 9 parts of D-sorbite, 18 parts of inositol, methionine 18
Part, 12 parts of lysine, purified water is 850 parts, and the weight of vitamin B1 is the 0.015% of mentioned component gross weight.
Further, in parts by weight, including 100 parts of Choline Chloride, 10 parts of D-sorbite, 20 parts of inositol, methionine
20 parts, 15 parts of lysine, purified water is 880 parts, and the weight of vitamin B1 is the 0.02% of mentioned component gross weight.
Present invention also offers the preparation method of above-mentioned livestock and poultry cultivation physical efficiency modifying agent, comprise the following steps:
Choline Chloride, D-sorbite, inositol, methionine, lysine, purified water and Wei Sheng are weighed respectively according to parts by weight
Plain B1, is stirred for producing livestock and poultry cultivation physical efficiency modifying agent to being completely dissolved.
Prepared present invention also offers above-mentioned livestock and poultry cultivation physical efficiency modifying agent for treating, improving the medicine of livestock and poultry liver function
Application in thing.
Further, when preparing for treating, improving the medicine of livestock and poultry liver function, the dosage of the modifying agent
For:
Take the modifying agent 500ml to be watered 500kg, continuously drink within 24 hours, be used in conjunction 3-5 days.
Further, the temperature of the water is no more than 40 degree.
Compared with prior art, the present invention at least has advantages below:
1. the livestock and poultry cultivation physical efficiency modifying agent that the present invention is provided can protect the liver of livestock and poultry from the infringement of toxicant.
2. the livestock and poultry cultivation physical efficiency modifying agent that the present invention is provided has the anti-oxidation function of strength, the liver of livestock and poultry can be protected
The dirty destruction from free radical.
3. the livestock and poultry cultivation physical efficiency modifying agent that the present invention is provided can promote the synthesis of protein, make liver in damaged condition thin
The quick self-healing of born of the same parents.
4. the livestock and poultry cultivation physical efficiency modifying agent that the present invention is provided can accelerate the generation of new liver cell, it can expire
Each organ of sufficient body, the demand of histocyte normal physiological activity.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, below to the technical side in the embodiment of the present invention
Case is clearly and completely described, it is clear that described embodiment is a part of embodiment of the invention, rather than whole realities
Apply example.Therefore, the detailed description below to embodiments of the invention is not intended to limit the scope of claimed invention, and
It is the selected embodiment for being merely representative of the present invention.Based on the embodiment in the present invention, those of ordinary skill in the art are not making
Go out the every other embodiment obtained under the premise of creative work, belong to the scope of protection of the invention.
Below by specific embodiment, the present invention is further illustrated.
In the formula of the present invention, the characteristics of each component and function is as follows:
Choline Chloride is used to treat fatty liver and hepatic sclerosis.Also serve as poultry and livestock feed additive, can stimulate laying eggs ovary more,
The weightening such as farrowing and poultry, fish.
Inositol plays the role of fat metabolism and cholesterol, reduction cholesterol, helps prevention of arterial hardening.Liver is removed in help
Fat.
Methionine can be used for preventing and treating the liver diseases such as chronic or oxyhepatitis, hepatic sclerosis, it can also be used to alleviate arsenic, three chloromethanes
The toxic reaction of the harmful substances such as alkane, carbon tetrachloride, benzene, pyridine and quinoline.
Lysine is to maintaining the normal growth and development of animal and the normal function of nervous system to have important effect.Feed
Middle shortage lysine can cause negative nitrogen effect, i.e. breaks down proteins and be discharged, and protein in feed from making full use of, is made
It is slow into growth of animal, it there is also the symptoms such as fatty liver, the reduction of sclerotin calcification rate.
Vitamin B1 is transformed into b1thiaminpyrophosphate (also known as cocarboxylase) in vivo, participates in the metabolism of sugar in vivo.Cause
During this Vitamin B1 deficiency, oxidation of the sugar in tissue is affected.It also has the effect for suppressing cholinesterase activity, lacks dimension
This enzymatic activity is too high during raw element B1, and acetylcholine (one of neurotransmitter) considerable damage makes nerve conduction be affected, and can cause
Gastrointestinal peristalsis is slow, and digestive secretion is reduced, the obstacle such as poor appetite, indigestion.
D-sorbite is used as stabilizer.
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, including 80-100 parts of Choline Chloride, D-sorbite 8-10
Part, 15-20 parts of inositol, 15-20 parts of methionine, 10-15 parts of lysine, purified water is 830-880 parts.Wherein, Choline Chloride
Content is 60~70mg/ml, and livestock and poultry cultivation physical efficiency modifying agent is yellow to brownish red supernatant liquid.
The present invention provide preferred embodiment in also include vitamin B1, the weight content of vitamin B1 is various
The 0.012%-0.02% of ingredient weight.
The preparation method for the livestock and poultry cultivation physical efficiency modifying agent that the present invention is provided, comprises the following steps:
Choline Chloride, D-sorbite, inositol, methionine, lysine, purified water and Wei Sheng are weighed respectively according to parts by weight
Plain B1, is stirred for producing livestock and poultry cultivation physical efficiency modifying agent to being completely dissolved.
Each embodiment that embodiment is seen below.
Embodiment 1
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 1.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in the embodiment 1 of table 1
Composition |
Content |
Choline Chloride |
80 parts |
D-sorbite |
8 parts |
Inositol |
15 parts |
Methionine |
15 parts |
Lysine |
10 parts |
Purified water |
830 parts |
Also include 0.012% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method, comprises the following steps:
Choline Chloride, D-sorbite, inositol, methionine, lysine, purified water and Wei Sheng are weighed respectively according to parts by weight
Plain B1, is stirred for producing livestock and poultry cultivation physical efficiency modifying agent to being completely dissolved.
Embodiment 2
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 2.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in the embodiment 2 of table 2
Composition |
Content |
Choline Chloride |
90 parts |
D-sorbite |
9 parts |
Inositol |
18 parts |
Methionine |
18 parts |
Lysine |
12 parts |
Purified water |
850 parts |
Also include 0.015% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method be the same as Example 1.
Embodiment 3
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 3.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in the embodiment 3 of table 3
Composition |
Content |
Choline Chloride |
100 parts |
D-sorbite |
10 parts |
Inositol |
20 parts |
Methionine |
20 parts |
Lysine |
15 parts |
Purified water |
880 parts |
Also include 0.02% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method be the same as Example 1.
Embodiment 4
A kind of livestock and poultry cultivation physical efficiency modifying agent, in parts by weight, its component and content are as shown in table 4.
The component and content of livestock and poultry cultivation physical efficiency modifying agent in the embodiment 4 of table 4
Composition |
Content |
Choline Chloride |
85 parts |
D-sorbite |
10 parts |
Inositol |
19 parts |
Methionine |
16 parts |
Lysine |
13 parts |
Purified water |
870 parts |
Also include 0.012% vitamin B1 of the gross weight of above-mentioned all components.
Its preparation method be the same as Example 1.
The measure of the livestock and poultry cultivation physical efficiency modifying agent Choline Chloride of embodiment 5
1) instrument used in experimentation, equipment include:
Constant Temp. Oven:Controllable temperature is in (105 ± 2) DEG C
Electronic balance:Scale division value is 0.0001g
Glass sand core crucible:Filter plate aperture (4~7) um.
2) preparation of reagents
Hydrochloric acid solution:Concentrated hydrochloric acid is with purified water according to V:V=1:4 prepare.
Sodium hydroxide solution:400g/L.
Reinecke's salt (diaminotetrathiocyanodiammonochromate) methanol solution:20g/L;2g Reinecke's salts are weighed, 100mL first is dissolved in
Alcohol, filtering;The solution, which is made into after 48h, can not use.
Methyl red-methylene blue mixing indicator solution.
3) analytical procedure
The livestock and poultry cultivation physical efficiency modifying agent 1g prepared by above-described embodiment 1-4 is weighed, 0.0002g is accurate to, is placed in 100ml
In beaker in tall form, plus 1 and drip the methylene blue mixing indicator solution of methyl red one, aubergine (pH5~pH6) is adjusted to hydrochloric acid solution, in
Less than 5 DEG C are cooled in ice-water bath, is continually maintained in ice-water bath, under agitation, 15ml Reinecke's salt methanol solutions are slowly added dropwise
Liquid, continues stirring reaction 30min;Still aging 30min again.Precipitation is transferred in advance in being dried at (105 scholar 2) DEG C to matter
In the constant glass core mandarin orange misfortune of amount, depressurize suction filtration, precipitation be washed with water 3~4 times, every time with water about 10ml.It will be equipped with precipitation
Glass sand core crucible move into (105 scholar 2) DEG C electrically heated drying cabinet in, dry 2h, take out.In being cooled to room temperature in drier, claim
Amount.
While determining, by the step identical with measure, done to being not added with sample using the reagent solution of identical quantity
Blank test.
4) interpretation of result
The content X for representing Choline Chloride with mass fraction is calculated by formula (1):
In formula, W1--- -- precipitates the numerical value of quality, and unit is gram (g);
W2--- -- blank test precipitates the numerical value of quality, and unit is gram (g);
The numerical value of W----- sample quality, unit is gram (g).
The arithmetic mean of instantaneous value for taking parallel determinations is measurement result, and the absolute of parallel determinations is not more than to deviation
0.5%.
The Choline Chloride for measuring livestock and poultry cultivation physical efficiency modifying agent in embodiment 1-4 according to the above method is respectively 56%,
63.2%th, 69.4% and 59.2%.
Hepatic function improvement effect of the livestock and poultry cultivation physical efficiency modifying agent of embodiment 6 to chronic hepatitis model mice
1) about 30g ICR mouse 30 are chosen, wherein 20,3 times a week to intraperitoneal administration carbon tetrachloride (V/V=
10% corn oil), first dosage is 330 μ l/kg, and second is 400 μ l/kg, is 500 μ l/kg later, is administered 6 weeks;
Other 10 are not administered and normally feed as normal group.
2) after 6 weeks, 20 mouse of administration are equally divided into two groups of A, B, every group is 10.Wherein A groups are model group;B
Group is administration group.The continuous intraperitoneal administration of mouse in B groups 5 days, is 1ml/kg livestock and poultry cultivation physical efficiency modifying agent every time.Wherein,
Prepared livestock and poultry cultivation physical efficiency modifying agent in livestock and poultry cultivation physical efficiency modifying agent selection example 3.The continuous abdominal cavity of mouse in A groups
Physiological saline 1ml/kg, successive administration 5 days is administered.
3) after administration terminates, from big venous blood collection, 4000rpm centrifugation 15min collect supernatant and are used as plasma sample.
4) AST (aspartate transaminase, GOT) and ALT (alanine aminotransferase, GPT) determined in plasma sample contains
Amount.Experimental result is as shown in table 5 below.
AST and ALT content in each group mice plasma sample of table 5
|
Normal group |
Model group |
Administration group |
AST |
36 |
193 |
41 |
ALT |
20 |
89 |
26 |
The data obtained is the average value of the AST and ALT numerical value measured by all mouse in each group in table 5.AST and
ALT is the important parameter for showing liver cell obstacle, as can be seen from Table 5, the livestock and poultry cultivation body prepared by the embodiment of the present invention 3
Energy modifying agent can effectively treat dysfunction of liver, improve liver function, and impaired liver cell can be made to return to normal level.
The livestock and poultry cultivation physical efficiency modifying agent that experiment other embodiments of the invention are provided has similar result.
Application of the livestock and poultry cultivation physical efficiency modifying agent of embodiment 7 in preparing for treating, improving the medicine of livestock and poultry liver function
When preparing for treating, improving the medicine of livestock and poultry liver function, the dosage of the modifying agent is:Take above-mentioned reality
The modifying agent 500ml for applying the preparation in a 1-4 is watered 500kg, continuously drinks within 24 hours, is used in conjunction 3-5 days.
The livestock and poultry cultivation physical efficiency improved application that the embodiment of the present invention 3 is provided is in certain pig farm of Jinan City.After, Ji
Increased weight of the pig on certain pig farm of Nanshi substantially than the pig on other pig farms is fast, wherein, control group uses water spray for control, experiment
Group sprays the livestock and poultry cultivation physical efficiency modifying agent prepared by embodiment 3, and Follow-up observation 1 year carries out daily observation and inspection to test pig
Survey, experimental result is as follows:
Compared with control group, the experimental group porcine respiratory disease incidence of disease is decreased obviously, and the incidence of disease is 18% in control group, and
The incidence of disease of experimental group is less than the adding weight of pig in 1%, and experimental group apparently higher than control group.
The livestock and poultry cultivation physical efficiency modifying agent that other embodiments of the invention are provided also has similar experimental result.
The livestock and poultry cultivation physical efficiency modifying agent of embodiment 7 is preparing the application in being used to treat the medicine of livestock and birds respiratory disease
When preparing the medicine for treating porcine respiratory disease, the dosage of the modifying agent is:Take the improvement
Agent 100ml is watered 500 kilograms, continuously drinks within 24 hours, is used in conjunction 3-4 days.
The livestock and poultry cultivation physical efficiency modifying agent that the embodiment of the present invention 3 is provided is applied to Jinan City pig farm, chooses this and supports
Grow within a porkling totally 58, its size, body weight difference 3%, its stochastic averagina is divided into 2 groups, one group is control group, and one group is
Experimental group.Wherein, the pig in experimental group, drinks livestock and poultry cultivation physical efficiency modifying agent prepared by embodiment 3 every month once;Control
Group normal water.Observe and record the upgrowth situation of pig, weighed after 7 months, wherein the increased weight of the pig in experimental group
The average value of amount is 1.34 times in control group, is found during raising, the feed intake of the porkling in experimental group substantially compares
It is big in control group, and porkling in experimental group is substantially without the situation of falling ill.
The livestock and poultry cultivation physical efficiency modifying agent that other embodiments of the invention are provided also has similar experimental result.
The livestock and poultry cultivation physical efficiency modifying agent that the present invention is provided can protect the liver of livestock and poultry from the infringement of toxicant, energy
Enough accelerate the production of new liver cell, each organ of body, histiocytic normal physiological activity need can be met
Ask so that porkling reduces rate of falling ill, improve resistance against diseases.
More than, it is only the present invention preferably embodiment, but the protection domain of invention is not limited thereto, and it is any ripe
Know those skilled in the art the invention discloses technical scope in, the change or replacement that can be readily occurred in should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be defined by the protection domain of claims.