CN107145718B - Method for identifying and evaluating wheat scab infection resistance - Google Patents
Method for identifying and evaluating wheat scab infection resistance Download PDFInfo
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- CN107145718B CN107145718B CN201710255309.9A CN201710255309A CN107145718B CN 107145718 B CN107145718 B CN 107145718B CN 201710255309 A CN201710255309 A CN 201710255309A CN 107145718 B CN107145718 B CN 107145718B
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- 241000209140 Triticum Species 0.000 title claims abstract description 38
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 38
- 208000015181 infectious disease Diseases 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 21
- 206010039509 Scab Diseases 0.000 title claims abstract description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 71
- 201000010099 disease Diseases 0.000 claims abstract description 70
- 238000011156 evaluation Methods 0.000 claims abstract description 12
- 208000035240 Disease Resistance Diseases 0.000 claims abstract description 5
- 239000012925 reference material Substances 0.000 claims abstract description 4
- 208000024891 symptom Diseases 0.000 claims abstract description 3
- 239000000463 material Substances 0.000 claims description 10
- 238000009331 sowing Methods 0.000 claims description 5
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 5
- 210000005069 ears Anatomy 0.000 claims description 3
- 230000000844 anti-bacterial effect Effects 0.000 claims description 2
- 239000003899 bactericide agent Substances 0.000 claims description 2
- 238000003973 irrigation Methods 0.000 claims description 2
- 230000002262 irrigation Effects 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 206010061217 Infestation Diseases 0.000 claims 1
- 230000002265 prevention Effects 0.000 claims 1
- 208000032625 disorder of ear Diseases 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000223218 Fusarium Species 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16Z—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS, NOT OTHERWISE PROVIDED FOR
- G16Z99/00—Subject matter not provided for in other main groups of this subclass
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Abstract
The invention discloses a method for identifying and evaluating gibberellic disease infection resistance of wheat, and belongs to the technical field of plant disease resistance identification and evaluation. Aims to provide a method for identifying and evaluating the scab infection resistance of wheat. Specifically, the method of the invention comprises the following steps: step one, establishing an identification garden in a perennial gibberellic disease retransmission area; step two, investigating infection points, wherein the infection points take florets with typical gibberellic disease symptoms as representatives, and calculating disease florets rate; thirdly, investigating the number of the ear of disease, and calculating the ear of disease rate; and step four, calculating an illness state index, wherein the illness state index is the disease florescence rate multiplied by the ear disease rate, and grading evaluation of scab infection resistance is carried out on the reference material according to the illness state index. The method provided by the invention provides an effective method for identifying the Type I resistance Type, better reflects the difference of wheat on gibberellic disease infection, and can accurately evaluate and grade the gibberellic disease infection resistance of the wheat.
Description
Technical Field
The invention particularly relates to a method for identifying and evaluating gibberellic disease infection resistance of wheat, and belongs to the technical field of plant disease resistance identification and evaluation.
Background
Wheat scab is one of the main diseases of wheat, the prevalence of scab can not only reduce the yield, but also affect the quality of wheat, and toxins contained in polluted grains can also harm the health of human beings and animals. Practice proves that breeding disease-resistant varieties is the most economic and effective means for preventing and treating the gibberellic disease. One of the bases of breeding is the evaluation of germplasm resources, so that the establishment of an accurate evaluation method has important significance for basic research on resistance to gibberellic disease.
Wheat scab resistance breeding is a worldwide problem. There are several manifestations of wheat resistance to gibberellic disease, and in 1963 Schroeder and Christensen proposed that wheat resistance to gibberellic disease could be divided into two categories, namely infection resistance (Type I) and spread resistance (Type II), which have been widely accepted and applied by breeders. The Type I resistance reflects the capability of wheat to resist the initial infection of pathogenic bacteria, the infection resistance identification is usually carried out by sowing wheat grains infected by gibberella in the field for inoculation or spraying gibberella spore liquid in mist before and after flowering or by means of natural morbidity, and the prevalence rate, namely the ear-of-disease rate, is taken as an identification index to be regarded as a suitable method for identifying the resistance. However, in the conventional identification and evaluation methods, materials exhibiting the same ear rate are not further finely classified. For the Type II of resistance extension, more researches are carried out, for example, the Chinese invention patent CN 104303866B discloses a method for identifying and evaluating the wheat scab extension resistance, and for the Type I of resistance infection with the highest utilization value in disease resistance, corresponding accurate identification technology and division standard are lacked, so that the full exploitation and utilization of resistance resources are limited to a great extent.
Disclosure of Invention
Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a method for identifying and evaluating resistance to wheat scab infection.
Specifically, the method of the invention comprises the following steps:
step one, establishing an identification garden in a perennial gibberellic disease retransmission area;
step two, investigating infection points, wherein the infection points take florets with typical gibberellic disease symptoms as representatives, and calculating disease florets rate;
thirdly, investigating the number of the ear of disease, and calculating the ear of disease rate;
and step four, calculating a Disease Index (DI), wherein the disease index is a disease florescence rate multiplied by a disease spike rate, and grading evaluation of scab infection resistance is carried out on the reference material according to the disease index.
Further, the second step in the method is specifically that the number of infection points is investigated on the 10 th day after flowering, the infection points are represented by florets with typical gibberellic disease, and the florets disease rate is calculated.
Further, the third step in the method is specifically that the number of ear of disease is investigated and the ear rate is calculated 20 days after flowering.
Further, the fourth step in the method is specifically to calculate an illness state index, wherein the illness state index is a disease small flower rate x an ear disease rate, the disease onset conditions of 3 control varieties are referred, the 3 control varieties are respectively Wangshuibai, Yangmai 158 and Yangyang 8545, the Wangshuibai is a disease-resistant control, the Yangmai 158 is an anti-resistance control, and the Yangyang 8545 is a susceptible control, and the grading evaluation of scab infection resistance is performed on the reference material according to the illness state index.
Further, the grading evaluation standard of the gibberellic disease infection resistance in the fourth step is specifically as follows:
disease-resistant Resistant (R): DI < DICK-R
anti-Moderately Resistant (MR): DICK-R≤DI＜DICK-MR
Medium Susceptibility (MS): DICK-MR≤DI＜DICK-S
Infection susceptable(s): DI ≧ DICK-S
Wherein DICK-RDisease resistance index; DICK-MRAn index of resistance to control disease; DICK-SIs the index of disease of the infected patients.
The invention has the beneficial effects that: the invention provides a method for identifying and evaluating gibberellic disease infection resistance of wheat, and provides an effective method for identifying Type I resistance types, which better reflects the difference of gibberellic disease infection of wheat and accurately evaluates and grades the gibberellic disease infection resistance of wheat.
Detailed Description
The following describes embodiments of the present invention:
in this example, a field natural disease method was used for identification. The selection of the test field is carried out in a gibberellic disease common area, a test field in agricultural science research institute of Nanping, Fujian province.
And (4) sowing in a proper period, and sowing materials with different growth periods in different periods, so that the flowering period is basically consistent, and a reliable identification result is obtained. Two replicates are set by using Wangshuibai as disease-resistant control, Yangmai 158 as medium-resistant control and Mianyang 8545 as susceptible control, and the arrangement of the materials in the inner replicate cells is designed according to a completely random block group. Each material is planted in 2 rows, the length of the furrow is 20m, the width is 1.5m, the row spacing is 30cm, 60 seeds are sowed in each row, and the seeds are sowed by manual ditching. Arranging a water drainage and irrigation ditch in the field, and erecting an anti-bird net after blooming. The bactericide is not used in the whole growth period, and other management is carried out according to the field.
After the wheat is spilt, randomly selecting 20 ears of wheat which blossoms on the same day from each material, hanging a plastic label on the lower ear stems, and counting the total floret number of each ear. The number of infection points was investigated on the 10 th day after the wheat rose, and the disease florescence rate (the percentage of disease florescence to the total florescence) was calculated by investigating the number of disease florescence, which is represented by the florescence with the typical gibberellic disease.
The number of head of scab of the identified material was investigated at 20 days after the wheat blossoms, and the head rate (percentage of the number of head to the total number of head to be investigated) was calculated. The total spike number was investigated to be not less than 100 spikes.
The disease index is calculated according to the comprehensive expression of the ear disease rate and the small disease flower (infection point) rate, and the infection resistance of the wheat variety (line) to the gibberellic disease is finely evaluated and graded. The results of evaluation of scab infection resistance and classification of wheat varieties from different sources are shown in table 1. From table 1, it can be seen that the disease indexes of 19 wheat varieties such as early wheat (i), 03P115 and lower Sichuan wheat are lower than those of disease-resistant control varieties, and disease resistance (R) is expressed and is an infection-resistant variety; the disease indexes of 46 varieties such as early wheat, red hull wheat, bald wheat and the like are lower than that of medium-resistant control varieties Yangmai 158 and higher than that of disease-resistant varieties, and the Medium Resistance (MR) is expressed and is an anti-infection variety; the disease index of 4 varieties of Triticum Aestivum and Triticum aestivum is lower than susceptible control and higher than resistant control, and the expression feeling (MS) is a feeling variety; bainong 9310 and Wumai No. 1 have disease indexes higher than those of susceptible contrast, and are susceptible (S) varieties.
TABLE 1
In the table: varieties with the same name and different sources are marked with numbers for distinguishing.
The foregoing is a preferred embodiment of the present invention, and it should be noted that it would be apparent to those skilled in the art that various modifications and enhancements can be made without departing from the principles of the invention, and such modifications and enhancements are also considered to be within the scope of the invention.
Claims (1)
1. A method for identifying and evaluating resistance to wheat scab infestation, said method comprising:
step one, establishing an identification garden in a perennial gibberellic disease retransmission area, identifying by adopting a field natural disease method, sowing materials with different growth periods in the identification garden in stages to ensure that the flowering periods are basically consistent so as to obtain a reliable identification result, setting two repetitions in total by taking expected water white as a disease-resistant contrast, Yangmai 158 as an anti-disease contrast and Mianyang 8545 as a disease-sensitive contrast, arranging repeated inner cell materials according to a completely random block design, wherein each material has 2 rows, each furrow has a length of 20m and a width of 1.5m, a row spacing of 30cm, sowing 60 seeds in each row, manually ditching, dibbling, arranging irrigation ditches in fields, erecting a bird prevention net after blooming, using no bactericide in the whole growth period, and carrying out other management according to fields;
step two, investigating infection points, wherein the infection points take florets with typical gibberellic disease symptoms as representatives, and calculating disease florets rate;
after the wheat is spilt, randomly selecting 20 ears of wheat which blossoms on the same day from each material, hanging a plastic label on lower stems of the ears, counting the total florets of each ear, and investigating infection points on the 10 th day after the wheat is spilt;
thirdly, investigating the number of the ear of disease, and calculating the ear of disease rate;
investigating and identifying the head of the wheat with scab disease on the 20 th day after the wheat blooms, and investigating that the total head is not less than 100 heads;
step four, calculating an illness state index, wherein the illness state index is a disease florescence rate multiplied by a disease ear rate, and grading evaluation of scab infection resistance is carried out on the reference material according to the illness state index;
the grading evaluation standard of the gibberellic disease infection resistance is specifically as follows:
disease resistance, abbreviated as R: DI < DICK-R
Medium resistance, abbreviated MR: DICK-R≤DI＜DICK-MR
The feeling, abbreviated as MS: DICK-MR≤DI＜DICK-S
Susceptible, abbreviated as S: DI ≧ DICK-S
Wherein DICK-RDisease resistance index; DICK-MRAn index of resistance to control disease; DICK-SIs the index of disease of the infected patients.
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| CN107145718B true CN107145718B (en) | 2021-01-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110163459B (en) * | 2018-03-23 | 2023-04-21 | 河南工业大学 | Multi-index evaluation method for constructing wheat quality grading |
| CN110338014B (en) * | 2019-08-15 | 2021-10-08 | 重庆市农业科学院 | Method for rapidly judging neck blast disease incidence grade of three-line rice breeding material |
| CN110991766B (en) * | 2019-12-20 | 2021-02-09 | 安徽省农业科学院作物研究所 | Method for predicting wheat scab occurrence risk |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103276047A (en) * | 2013-05-30 | 2013-09-04 | 四川农业大学 | Method for rapidly identifying scab resistance of wheat |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103276047A (en) * | 2013-05-30 | 2013-09-04 | 四川农业大学 | Method for rapidly identifying scab resistance of wheat |
Non-Patent Citations (4)
| Title |
|---|
| 145 份小麦品种(系) 的赤霉病抗性评价;苏双飞 等;《安徽科技学院学报》;20151231;第29卷(第2期);第14-15、17页 * |
| Scab of Wheat and Barley：A Re-emerging Disease of Devasting Impact;Marcia McMullen 等;《plant disease》;20070222;全文 * |
| 小麦抗赤霉病性的生化研究及其机制的探讨;王雅平 等;《作物学报》;19940531;第20卷(第3期);第328页 * |
| 小麦抗赤霉病性的配合力和因子分析;肖世和 等;《湖南农学院学报》;19850331(第1期);全文 * |
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