CN107145718A - A kind of method identified and evaluate wheat scab infection resistance - Google Patents
A kind of method identified and evaluate wheat scab infection resistance Download PDFInfo
- Publication number
- CN107145718A CN107145718A CN201710255309.9A CN201710255309A CN107145718A CN 107145718 A CN107145718 A CN 107145718A CN 201710255309 A CN201710255309 A CN 201710255309A CN 107145718 A CN107145718 A CN 107145718A
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- disease
- resistance
- head blight
- little hua
- infection
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- 241000209140 Triticum Species 0.000 title claims abstract description 41
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 41
- 208000015181 infectious disease Diseases 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 27
- 206010039509 Scab Diseases 0.000 title claims abstract description 15
- 201000010099 disease Diseases 0.000 claims abstract description 66
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 66
- 238000011835 investigation Methods 0.000 claims abstract description 12
- 238000011156 evaluation Methods 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 10
- 238000002474 experimental method Methods 0.000 claims abstract description 5
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 238000009331 sowing Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000016571 aggressive behavior Effects 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16Z—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS, NOT OTHERWISE PROVIDED FOR
- G16Z99/00—Subject matter not provided for in other main groups of this subclass
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Abstract
The invention discloses a kind of method identified and evaluate wheat scab infection resistance, belong to plant disease-resistant identification and assessment technique field.Purpose is to provide a kind of method identified and evaluate wheat scab infection resistance.Specifically, the method for the present invention includes：Step one retransmits area in head blight and sets up identification garden throughout the year；Points are infected in step 2 investigation, and infection court calculates disease little Hua rates using the little Hua with typical head blight illness as representative；Step 3 investigation morbidity spike number, calculates disease tassel yield；Step 4 calculates disease index, and disease index=disease little Hua rates × disease tassel yield carries out the grading evaluation of head blight infection resistance with disease index to material of participating in the experiment.The method that the present invention is provided, it is proposed that the effective ways of identification Type I resistance types, preferably reacts the difference that wheat infects to head blight, and wheat scab infection resistance can be carried out precisely to evaluate and be classified.
Description
Technical field
Present invention relates particularly to a kind of method identified and evaluate wheat scab infection resistance, belong to plant disease-resistant identification
With assessment technique field.
Background technology
Wheat scab is one of Major Diseases of wheat, and the prevalence of head blight can not only reduce yield, and influence small
Wheat quality, the toxin that contaminated seed contains can also endanger the health of human and animal.It was verified that breeding resistant variety is anti-
Control the most economical effective means of head blight.And one of basis of breeding is exactly the evaluation to germ plasm resource, therefore, establish accurately
Evaluation method is significant for carrying out anti gibberellic disease basic research.
Wheat Breeding For Scab Resistance is a global problem.Wheat has many forms to the resistance of head blight,
Schroeder in 1963 and Christensen propose that wheat can divide into the resistance of head blight to two classes, that is, are divided into anti-invade
Contaminate (Type I) and anti-extension (Type II) two types, above-mentioned differentiation by breeding man widely receive and apply.Its
In, Type I Resistant response wheats resist the ability of pathogen First aggression, anti-to infect identification and broadcasted sowing by red mould frequently with field
The wheat inoculation of bacterium infection sprays gibberella spore liquid in SPRING WHEAT BEFORE AND AFTER FLOWERING atomizing or relies on natural occurrence, with generally rate that is, disease
Fringe rate is considered as identifying a kind of appropriate method of this kind of resistance as identification of indicator.But, it is right in existing identification and evaluation method
The material of identical disease tassel yield is showed, further fine division is not done.For anti-extension (Type II) type, the comparison of research
Many, such as B of Chinese invention patent CN 104303866 disclose a kind of method for identifying and evaluating wheat scab extension resistance,
And for infecting (Type I resistances) type disease-resistant middle value highest is anti-, still lack corresponding precisely identification technology and
The criteria for classifying, greatly limit the abundant excavation of Resistance resource with utilizing.
The content of the invention
Therefore, the present invention seeks to solve above mentioned problem of the prior art, there is provided one kind identification and evaluation wheat are red
The method of mildew infection resistance.
Specifically, the method for the present invention includes：
Step one retransmits area in head blight and sets up identification garden throughout the year；
Points are infected in step 2 investigation, and infection court calculates disease little Hua using the little Hua with typical head blight illness as representative
Rate；
Step 3 investigation morbidity spike number, calculates disease tassel yield；
Step 4 calculates disease index (disease index, DI), disease index=disease little Hua rates × disease tassel yield, with disease
Feelings exponent pair participate in the experiment material carry out head blight infection resistance grading evaluation.
Further, step 2 is specifically, Post flowering the 10th day in methods described, and points are infected in investigation, and infection court is to have
The little Hua for having typical head blight illness is representative, calculates disease little Hua rates.
Further, in methods described step 3 specifically, Post flowering the 20th day, investigation morbidity spike number, calculate disease tassel yield.
Further, in methods described step 4 specifically, calculate disease index, disease index=disease little Hua rates × disease fringe
Rate, with reference to the incidence of 3 check varieties, 3 check varieties are respectively Wangshuibai, Yangmai No.158 and Mianyang 8545, its
Middle Wangshuibai be it is disease-resistant control, Yangmai No.158 be moderate resistance control, Mianyang 8545 be susceptible control, with disease index to material of participating in the experiment
Carry out the grading evaluation of head blight infection resistance.
Further, the grading evaluation criteria of head blight infection resistance is specially in the step 4：
Disease-resistant Resistant (R)：DI ＜ DICK-R
Moderate resistance Moderately resistant (MR)：DICK-R≤ DI ＜ DICK-MR
Middle sense Moderately susceptible (MS)：DICK-MR≤ DI ＜ DICK-S
Susceptible Susceptible (S)：DI≥DICK-S
Wherein, DICK-RFor disease-resistant control disease index；DICK-MRDisease index is compareed for moderate resistance；DICK-SFor susceptible control
Disease index.
The beneficial effects of the present invention are：The present invention provides a kind of side for identifying and evaluating wheat scab infection resistance
Method, it is proposed that the effective ways of identification Type I resistance types, the difference that more preferably reaction wheat infects to head blight, precisely to small
Wheat head blight infection resistance is evaluated and is classified.
Embodiment
The embodiment to the present invention is illustrated below：
In the present embodiment, identified using field natural occurrence method.Experimental plot selection is head blight Chang Faqu-Fujian
Experimental plot is carried out in Nanping City's Institute of agricultural sciences institute of province.
Proper seeding, breeding time different material wants interval sowing, so that the florescence is basically identical, reliably to be identified
As a result.Using Wangshuibai as disease-resistant control, Yangmai No.158 compares for moderate resistance, and Mianyang 8545 is susceptible control, and two repetitions are set altogether,
Randomized complete-block design is pressed in the arrangement of cell material in repeating.Each row of material kind 2, furrow long 20m, wide 1.5m, line-spacing 30cm,
Often row sowing 60, artificial trench digging puts seed.Field sets irrigation and drainage ditch, and Post flowering sets up bird prevention net.Whole breeding time is without using killing
Microbial inoculum, other management are carried out by crop field.
After wheat heading, every part of material randomly selects the fringe of the wheat head 20 bloomed on the same day, and extension plastics are hung on stem under fringe
Card mark, counts above-mentioned each total little Hua numbers of the wheat head.Points are infected in investigation in the 10th day after wheat flowering, and infection court is with allusion quotation
The little Hua of type head blight illness is representative, that is, investigates disease little Hua numbers, and calculating disease little Hua rates, (sick little Hua numbers account for total little Hua numbers
Percentage).
The head blight morbidity spike number of the 20th day investigation expert evidence after wheat flowering, calculating disease tassel yield, (sick spike number is accounted for
Investigate the percentage of total spike number).Total spike number is investigated no less than 100 fringes.
The present invention calculates disease index, to wheat breed with disease tassel yield and the general performance of disease little Hua (infecting points) rate
(being) head blight infection resistance has carried out fine evaluation and classification.Separate sources wheat breed head blight infection resistance is evaluated with dividing
Level result is as shown in table 1.As can be seen from Table 1 early wheat 1., 19 wheat breed disease indexs such as 03P115, lower another name for Sichuan Province wheat it is low
In disease-resistant check variety Wangshuibai, show disease-resistant (R), kind is infected to be anti-；Early wheat 2., red shell shaven head, 46, bare headed wheat etc.
Kind disease index is less than moderate resistance check variety Yangmai No.158, higher than disease-resistant variety Wangshuibai, and performance moderate resistance (MR), is that moderate resistance is invaded
Contaminate kind；4 kinds such as iron head wheat, Taiwan wheat, disease index is less than susceptible control, higher than moderate resistance control, feels in performance
(MS), it is middle sense kind；No. 1 disease index of hundred agricultures 9310 and military wheat is higher than susceptible control, is susceptible (S) kind.
Table 1
In table：Title is identical, originate different kind addend word marks to distinguish.
Above is the preferred embodiment of the present invention, it is noted that for those skilled in the art,
Under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should be regarded as this
The protection domain of invention.
Claims (5)
1. a kind of method identified and evaluate wheat scab infection resistance, it is characterised in that methods described includes：
Step one retransmits area in head blight and sets up identification garden throughout the year；
Points are infected in step 2 investigation, and infection court calculates disease little Hua rates using the little Hua with typical head blight illness as representative；
Step 3 investigation morbidity spike number, calculates disease tassel yield；
Step 4 calculates disease index, and disease index=disease little Hua rates × disease tassel yield is carried out red with disease index to material of participating in the experiment
The grading evaluation of mildew infection resistance.
2. identification as claimed in claim 1 and the method for evaluating wheat scab infection resistance, it is characterised in that methods described
Middle step 2 is specifically, points are infected in Post flowering the 10th day, investigation, and infection court is using the little Hua with typical head blight illness as generation
Table, calculates disease little Hua rates.
3. identification as claimed in claim 2 and the method for evaluating wheat scab infection resistance, it is characterised in that methods described
Middle step 3 is specifically, Post flowering the 20th day, investigation morbidity spike number, calculate disease tassel yield.
4. identification as claimed in claim 1 and the method for evaluating wheat scab infection resistance, it is characterised in that methods described
Middle step 4 is specifically, calculating disease index, disease index=disease little Hua rates × disease tassel yield, with reference to the morbidity of 3 check varieties
Situation, 3 check varieties are respectively Wangshuibai, Yangmai No.158 and Mianyang 8545, and wherein Wangshuibai is disease-resistant control, Yang Mai
158 be that moderate resistance is compareed, Mianyang 8545 is susceptible control, carries out the classification of head blight infection resistance to material of participating in the experiment with disease index
Evaluate.
5. identification as claimed in claim 1 and the method for evaluating wheat scab infection resistance, it is characterised in that the step
The grading evaluation criteria of head blight infection resistance is specially in four：
It is disease-resistant, referred to as R：DI ＜ DICK-R
Moderate resistance, referred to as MR：DICK-R≤ DI ＜ DICK-MR
Middle sense, referred to as MS：DICK-MR≤ DI ＜ DICK-S
It is susceptible, referred to as S：DI≥DICK-S
Wherein, DICK-RFor disease-resistant control disease index；DICK-MRDisease index is compareed for moderate resistance；DICK-SRefer to for the susceptible control state of an illness
Number.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710255309.9A CN107145718B (en) | 2017-04-19 | 2017-04-19 | Method for identifying and evaluating wheat scab infection resistance |
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| CN201710255309.9A CN107145718B (en) | 2017-04-19 | 2017-04-19 | Method for identifying and evaluating wheat scab infection resistance |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110163459A (en) * | 2018-03-23 | 2019-08-23 | 河南工业大学 | A method of building multiple index evaluation model is classified wheat quality |
| CN110338014A (en) * | 2019-08-15 | 2019-10-18 | 重庆市农业科学院 | A kind of method of quick judgement three lines paddy breeding material panicle blast morbidity grade |
| CN110991766A (en) * | 2019-12-20 | 2020-04-10 | 安徽省农业科学院作物研究所 | Method for predicting wheat scab occurrence risk |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103276047A (en) * | 2013-05-30 | 2013-09-04 | 四川农业大学 | Method for rapidly identifying scab resistance of wheat |
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2017
- 2017-04-19 CN CN201710255309.9A patent/CN107145718B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103276047A (en) * | 2013-05-30 | 2013-09-04 | 四川农业大学 | Method for rapidly identifying scab resistance of wheat |
Non-Patent Citations (8)
| Title |
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| MARCIA MCMULLEN 等: "Scab of Wheat and Barley：A Re-emerging Disease of Devasting Impact", 《PLANT DISEASE》 * |
| 徐健容 等: "小麦近缘种属对赤霉病菌的抗性评价", 《四川农业大学学报》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110163459A (en) * | 2018-03-23 | 2019-08-23 | 河南工业大学 | A method of building multiple index evaluation model is classified wheat quality |
| CN110163459B (en) * | 2018-03-23 | 2023-04-21 | 河南工业大学 | Multi-index evaluation method for constructing wheat quality grading |
| CN110338014A (en) * | 2019-08-15 | 2019-10-18 | 重庆市农业科学院 | A kind of method of quick judgement three lines paddy breeding material panicle blast morbidity grade |
| CN110338014B (en) * | 2019-08-15 | 2021-10-08 | 重庆市农业科学院 | Method for rapidly judging neck blast disease incidence grade of three-line rice breeding material |
| CN110991766A (en) * | 2019-12-20 | 2020-04-10 | 安徽省农业科学院作物研究所 | Method for predicting wheat scab occurrence risk |
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| CN107145718B (en) | 2021-01-01 |
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