CN107140742A - A kind of mixed type algae water cleanser and its application - Google Patents
A kind of mixed type algae water cleanser and its application Download PDFInfo
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- CN107140742A CN107140742A CN201710415337.2A CN201710415337A CN107140742A CN 107140742 A CN107140742 A CN 107140742A CN 201710415337 A CN201710415337 A CN 201710415337A CN 107140742 A CN107140742 A CN 107140742A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of mixed type algae water cleanser and its application, the clearing machine composition and its parts by weight are：37 parts of chlorella pyrenoidosa, 5 10 parts of anabena.The preparation method of the cleanser includes the preparation of chlorella pyrenoidosa, the preparation of anabena, the immobilization of frustule.Have the beneficial effect that：The mixed type algae water cleanser of preparation is the frustule of immobilization, and anabolic activity is high, and aging is slow, can effectively remove ammonia nitrogen, nitrate and the nitrite in water body, improves aquatic environment；The cleanser process is simple, and product quality stability is good, cheap, and application effect is good, with good economic benefit.
Description
Technical field
The present invention relates to Environmental Technology biological field, more particularly, to a kind of mixed type algae water cleanser and its should
With.
Background technology
At present, the aquaculture area of China reaches millions of hectares, due to highdensity cultivation, is needed in breeding process
Substantial amounts of feed, antibiotic are added into water body.The metabolin of food-residue and aquaculture organism can cause water body COD to raise；
The decomposition of the organic matter such as substantial amounts of food-residue, excrement can cause the oxygen content of water body to reduce in water body；And due in water body
Nitrogen can form ammonia nitrogen, nitre in the materials such as protein, amino acid in the ammonification of microorganism and nitrification, the feed added
The material such as hydrochlorate and nitrite, this results in the deterioration of breeding water body environment, influences aquatile healthy growth.
Prior art, such as Authorization Notice No. are the B of CN 104671432 Chinese invention patent, disclose a kind of aquatic products and support
Water body cleansing agent is grown, includes bacillus amyloliquefaciens IAE zymotic fluid, powdered rice hulls and Paris white, the invention avoids the region of rivers and lakes
Breeding water body secondary pollution and region of rivers and lakes breeding water body pollute with the environment water that surrounding environment water body is exchanged and caused, but the hair
Bright complex technical process, production cost is high.
The content of the invention
It is an object of the invention to provide the mixed type algae that a kind of purifying water effect is good, preparation process is few, production cost is low
Water body cleansing agent.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of mixed type algae water is net
Agent, the composition and its parts by weight of the cleanser are：3-7 parts of chlorella pyrenoidosa, 5-10 parts of anabena.Chlorella pyrenoidosa pair
The absorbability of ammonia nitrogen is strong, anabena preferential absorption NO3-N and NO2-N, they is mixed to play its synergy,
There is more preferable catharsis to water body, the economic value of product is improved.
Preferably, a kind of mixed type algae water cleanser, its preparation method includes the preparation of chlorella pyrenoidosa, fish
The preparation of raw meat algae, the immobilization of frustule.Process is simple, and product quality stability is good, cheap, and application effect is good,
With good economic benefit.
Preferably, in the preparation process of chlorella pyrenoidosa, 1-5 parts of chlorella pyrenoidosa kinds are inoculated in containing 15-
In the conical flask of 20 parts of SE culture mediums, in 20-24 DEG C of temperature, illumination 8-10h, no light 8-10h, illuminance 1500-
Cultivated under 1900lx, the chlorella pyrenoidosa in logarithmic phase is centrifuged into 10-14min under 3600-3800r/min, supernatant is abandoned
Liquid, obtains chlorella pyrenoidosa cell, and process is sterile working.Under the conditions of being somebody's turn to do, chlorella pyrenoidosa vitro growth rates are most fast, institute
The chlorella pyrenoidosa cell yield of acquisition is high, and activity is high, and the absorbability to ammonia nitrogen in water body is strong.
Preferably, in the preparation process of anabena, 2-8 parts of anabena kinds are inoculated in containing 15-20 parts of SE culture mediums
Conical flask in, in 20-24 DEG C of temperature, illumination 10-12h, no light 10-12h are cultivated under illuminance 2100-2300lx, will
Anabena in logarithmic phase centrifuges 10-14min under 3600-3800r/min, abandons supernatant, obtains anabena cell, and process is
Sterile working.Under the conditions of being somebody's turn to do, anabena vitro growth rates are most fast, and the anabena cell yield obtained is high, and activity is high, to water
The absorbability of NO3-N and NO2-N is strong in body.
Preferably, in the immobilization process of frustule, 3-7 parts of chlorella pyrenoidosa cells, 5-10 parts of anabenas are thin
Born of the same parents, 1-4 parts of bioactive peptides and 5-10 parts of mass fractions uniformly mix for 1.5-2.5% calcium alginate solution, use syringe
Mixed liquor is drawn, No. 4 syringe needles are put, at the 0.1-0.13mol/L calcium chloride solution liquid levels 18-20cm that distance is pre-cooled,
Instill and form a diameter of 2-3mm bead, the gel 4-9h at 1-5 DEG C takes out particle standby with distilled water flushing 2-3 times.
Compared to suspended state algae, the algae handled by immobilization is higher to the clearance of nitrogen in the style of calligraphy, and improves algae pair
The tolerance of poisonous substance, organic pollution, drastically increases the quality of product.
Preferably, in the immobilization process of frustule, the amino acid sequence of the bioactive peptide added is：
HSHACNCSR.The gel ball that the immobilization of frustule is formed, is easily destroyed, what is added is active short by microorganism and phosphate
Peptide not only has anti-microbial property, and forms complex compound with phosphate, reduces the influence of microorganism and phosphate to product.
Mixed type algae water cleanser prepared by the present invention is applied to：The purified treatment of breeding water body.
Preferably, the decontamination process of breeding water body is：One is sprayed with the full pond of mixed type algae purification agent within every 60 days
Secondary, the consumption of mixed type algae purification agent is 5-10 parts.
Compared with prior art, the advantage of the invention is that：The mixed type algae water cleanser of preparation is immobilization
Frustule, anabolic activity is high, and aging is slow, can effectively remove ammonia nitrogen, nitrate and the nitrite in water body, improves
Aquatic environment；The bioactive peptide added in immobilization process makes the Water body cleansing agent more stablize, and extends its efficacy window；
The cleanser process is simple, and product quality stability is good, cheap, and application effect is good, with good economic benefit.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
A kind of mixed type algae water cleanser, the composition and its parts by weight of the cleanser are：3 parts of chlorella pyrenoidosa, fish raw meat
5 parts of algae, preparation comprises the following steps：(1) preparation of chlorella pyrenoidosa：Under aseptic processing environment, by 1 part of pyrenoids bead
Algae kind is inoculated in the conical flask containing 20 parts of SE culture mediums, and conical flask is placed in biological illumination incubator, control temperature
Spend for 20 DEG C, use CWF lamp for light source, it is 1500lx, illumination 8h, dark 8h to control illuminance, is cultivated to logarithmic phase,
Concentration is suitable, and chlorella pyrenoidosa is gone in centrifuge tube, and setting rotating speed is 3600r/min, 10min is centrifuged, by supernatant
Liquid is poured into waste liquid barrel, and surplus materials is chlorella pyrenoidosa cell；(2) preparation of anabena：, will under aseptic processing environment
1 part of anabena kind is inoculated in the conical flask containing 20 parts of SE culture mediums, and conical flask is placed in biological illumination incubator,
It is 20 DEG C to control temperature, uses CWF lamp for light source, and it is 2100lx, illumination 10h, dark 10h to control illuminance, and culture is extremely
Logarithmic phase, concentration is suitable, and anabena is gone in centrifuge tube, and setting rotating speed is 3600r/min, 10min is centrifuged, by supernatant
Liquid is poured into waste liquid barrel, and surplus materials is chlorella pyrenoidosa cell；(3) immobilization of frustule：Obtained frustule is used
Physiological saline, which suspends, to be washed 2 times, removes frustule surface medium, then frustule is collected by centrifugation, and takes 3 parts of chlorella pyrenoidosas thin
Born of the same parents and 5 parts of anabena mixing with cells, add mass fraction and are 1.5% calcium alginate solution, then are slowly stirred 1 part of work of lower addition
Property small peptide, with syringe draw mixed liquor, put No. 4 syringe needles, the 0.1mol/L calcium chloride solution liquid levels pre-cooled in distance
At 20cm, instill and form a diameter of 3mm bead, the gel 8h at 4 DEG C takes out particle standby with distilled water flushing 3 times.Phase
Than in suspended state algae, the algae handled by immobilization is higher to the clearance of nitrogen in the style of calligraphy, and improves algae to poison
The tolerance of thing, organic pollution, drastically increases the quality of product.
Aquaculture is used it for, is sprayed once with the full pond of mixed type algae purification agent within every 60 days, mixed type algae purification
The consumption of agent is 5 parts.
Embodiment 2：
A kind of mixed type algae water cleanser, the composition and its parts by weight of the cleanser are：4 parts of chlorella pyrenoidosa, fish raw meat
6 parts of algae, preparation comprises the following steps：
1) preparation of chlorella pyrenoidosa：2 parts of chlorella pyrenoidosa kinds are inoculated in the conical flask containing 16 parts of SE culture mediums
In, in 22 DEG C of temperature, illumination 9h, no light 9h are cultivated under illuminance 1600lx, the chlorella pyrenoidosa in logarithmic phase are existed
12min is centrifuged under 3700r/min, supernatant is abandoned, chlorella pyrenoidosa cell is obtained, process is sterile working.Under the conditions of being somebody's turn to do, albumen
The core chlorella cells speed of growth is most fast, and the chlorella pyrenoidosa cell yield obtained is high, and activity is high, to ammonia nitrogen in water body
Absorbability is strong；
2) preparation of anabena：3 parts of anabena kinds are inoculated in the conical flask containing 16 parts of SE culture mediums, in temperature 22
DEG C, illumination 11h, no light 11h are cultivated under illuminance 2200lx, the anabena in logarithmic phase are centrifuged under 3700r/min
12min, abandons supernatant, obtains anabena cell, and process is sterile working.Under the conditions of being somebody's turn to do, anabena vitro growth rates are most fast, institute
The anabena cell yield of acquisition is high, and activity is high, and the absorbability to NO3-N and NO2-N in water body is strong；
3) immobilization of frustule：By 4 parts of chlorella pyrenoidosa cells, 6 parts of anabena cells, 2 parts of bioactive peptides and 6 parts of matter
Amount fraction uniformly mixes for 2% calcium alginate solution, draws mixed liquor with syringe, puts No. 4 syringe needles, cold in advance in distance
But at 0.11mol/L calcium chloride solution liquid levels 20cm, the bead for forming a diameter of 3mm is instilled, gel 8h, takes at 4 DEG C
Go out particle distilled water flushing 3 times standby.Compared to suspended state algae, the algae handled by immobilization goes to nitrogen in the style of calligraphy
Except rate is higher, and algae is improved to poisonous substance, the tolerance of organic pollution, drastically increase the quality of product.
Aquaculture is used it for, is sprayed once with the full pond of mixed type algae purification agent within every 60 days, mixed type algae purification
The consumption of agent is 6 parts.
Embodiment 3：
A kind of mixed type algae water cleanser, the composition and its parts by weight of the cleanser are：7 parts of chlorella pyrenoidosa, fish raw meat
10 parts of algae, preparation comprises the following steps：
1) preparation of chlorella pyrenoidosa：5 parts of chlorella pyrenoidosa kinds are inoculated in the conical flask containing 15 parts of SE culture mediums
In, in 24 DEG C of temperature, illumination 10h, no light 10h are cultivated under illuminance 1900lx, by the chlorella pyrenoidosa in logarithmic phase
14min is centrifuged under 3800r/min, supernatant is abandoned, chlorella pyrenoidosa cell is obtained, process is sterile working.Under the conditions of being somebody's turn to do, egg
The white nucleus chlorella cells speed of growth is most fast, and the chlorella pyrenoidosa cell yield obtained is high, and activity is high, to ammonia nitrogen in water body
Absorbability it is strong；
2) preparation of anabena：8 parts of anabena kinds are inoculated in the conical flask containing 15 parts of SE culture mediums, in temperature 24
DEG C, illumination 12h, no light 12h are cultivated under illuminance 2300lx, the anabena in logarithmic phase are centrifuged under 3800r/min
14min, abandons supernatant, obtains anabena cell, and process is sterile working.Under the conditions of being somebody's turn to do, anabena vitro growth rates are most fast, institute
The anabena cell yield of acquisition is high, and activity is high, and the absorbability to NO3-N and NO2-N in water body is strong；
3) immobilization of frustule：By 7 parts of chlorella pyrenoidosa cells, 10 parts of anabena cells, 4 parts of bioactive peptides and 10 parts
Mass fraction uniformly mixes for 2.5% calcium alginate solution, draws mixed liquor with syringe, puts No. 4 syringe needles, pre- in distance
At the 0.13mol/L calcium chloride solution liquid levels 20cm first cooled down, the bead for forming a diameter of 3mm, the gel at 4 DEG C are instilled
8h, takes out particle standby with distilled water flushing 3 times.Compared to suspended state algae, the algae handled by immobilization is in the style of calligraphy
The clearance of nitrogen is higher, and improves algae to poisonous substance, the tolerance of organic pollution, drastically increases the product of product
Matter.
Aquaculture is used it for, is sprayed once with the full pond of mixed type algae purification agent within every 60 days, mixed type algae purification
The consumption of agent is 10 parts.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Culturing high-quality seedling election effects institute of Zhoushan Putuo Xinghai County
<120>A kind of mixed type algae water cleanser and its application
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 9
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Asn Cys Ser Arg
1 5
Claims (7)
1. a kind of mixed type algae water cleanser, it is characterised in that：The composition and its parts by weight of the Water body cleansing agent be：Egg
3-7 parts of white nucleus chlorella, 5-10 parts of anabena.
2. a kind of mixed type algae water cleanser according to claim 1, its preparation method includes chlorella pyrenoidosa
Preparation, the preparation of anabena, the immobilization of frustule, it is characterised in that：Added in the immobilization process of the frustule
A kind of bioactive peptide.
3. a kind of preparation method of mixed type algae water cleanser according to claim 2, it is characterised in that：The algae
In the immobilization process of cell, the amino acid sequence of the bioactive peptide added is：HSHACNCSR.
4. a kind of preparation method of mixed type algae water cleanser according to claim 2, it is characterised in that：The algae
In the immobilization process of cell, by 3-7 parts of chlorella pyrenoidosa cells, 5-10 parts of anabena cells, 1-4 parts of bioactive peptides and
5-10 parts of mass fractions are mixed for 1.5-2.5% calcium alginate solution, stir 15-20min, and mixed liquor is drawn with syringe,
No. 4 syringe needles are put, at the 0.1-0.13mol/L calcium chloride solution liquid levels 18-20cm that distance is pre-cooled, instills and forms straight
Footpath is 2-3mm bead, and the gel 4-9h at 1-5 DEG C takes out particle standby with distilled water flushing 2-3 times.
5. a kind of preparation method of mixed type algae water cleanser according to claim 2, it is characterised in that：The egg
In the preparation process of white nucleus chlorella, 1-5 parts of chlorella pyrenoidosa kinds are inoculated in the triangle containing 15-20 parts of SE culture mediums and burnt
In bottle, in 20-24 DEG C of temperature, illumination 8-10h, no light 8-10h cultivate under illuminance 1500-1900lx, will be in logarithmic phase
Chlorella pyrenoidosa centrifuge 10-14min under 3600-3800r/min, abandon supernatant, obtain chlorella pyrenoidosa cell, process
For sterile working.
6. a kind of preparation method of mixed type algae water cleanser according to claim 2, it is characterised in that：It is described to incite somebody to action
2-8 parts of anabena kinds are inoculated in the conical flask containing 15-20 parts of SE culture mediums, in 20-24 DEG C of temperature, illumination 10-12h,
Under no light 10-12h, illuminance 2100-2300lx cultivate, by the anabena in logarithmic phase under 3600-3800r/min from
Heart 10-14min, abandons supernatant, obtains anabena cell, and process is sterile working.
7. a kind of application of mixed type algae water cleanser, it is characterised in that：Use it for aquaculture, every 60 days with mixing
Once, the consumption of mixed type algae purification agent is 5-10 parts to the full pond sprinkling of type algae purification agent.
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Cited By (8)
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| CN107815447A (en) * | 2017-12-11 | 2018-03-20 | 陈英 | A kind of compound algae microballoon of immobilization and its application |
| CN107827328A (en) * | 2017-12-14 | 2018-03-23 | 陈英 | A kind of technique for repairing breeding wastewater |
| CN107935189A (en) * | 2017-12-14 | 2018-04-20 | 陈英 | A kind of technique using algae microballoon processing sanitary sewage |
| CN107937382A (en) * | 2017-12-26 | 2018-04-20 | 安徽仁维环保工程科技有限公司 | A kind of preparation method of immobilized microalgae |
| CN107974411A (en) * | 2017-12-26 | 2018-05-01 | 安徽仁维环保工程科技有限公司 | A kind of preparation method of Immobilized hyphae microballoon |
| CN109837230A (en) * | 2019-04-17 | 2019-06-04 | 中国科学院水生生物研究所 | Bacillus amyloliquefaciens Y1711, cultural method and its application |
| CN110479080A (en) * | 2019-08-21 | 2019-11-22 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect drop haze agent of algae |
| CN114752503A (en) * | 2022-05-06 | 2022-07-15 | 阿尔格生命科学(江苏)有限公司 | Production process of algae active high-efficiency heavy metal degradation agent |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107815447A (en) * | 2017-12-11 | 2018-03-20 | 陈英 | A kind of compound algae microballoon of immobilization and its application |
| CN107815447B (en) * | 2017-12-11 | 2021-09-14 | 杭州富阳佳畅机械有限公司 | Immobilized composite algae microspheres and application thereof |
| CN107827328A (en) * | 2017-12-14 | 2018-03-23 | 陈英 | A kind of technique for repairing breeding wastewater |
| CN107935189A (en) * | 2017-12-14 | 2018-04-20 | 陈英 | A kind of technique using algae microballoon processing sanitary sewage |
| CN112537889A (en) * | 2017-12-14 | 2021-03-23 | 陈英 | Process for repairing aquaculture wastewater |
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| CN107974411A (en) * | 2017-12-26 | 2018-05-01 | 安徽仁维环保工程科技有限公司 | A kind of preparation method of Immobilized hyphae microballoon |
| CN109837230A (en) * | 2019-04-17 | 2019-06-04 | 中国科学院水生生物研究所 | Bacillus amyloliquefaciens Y1711, cultural method and its application |
| CN110479080A (en) * | 2019-08-21 | 2019-11-22 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect drop haze agent of algae |
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Application publication date: 20170908 |