CN107137286A - A kind of preparation method and applications of fermented ginseng liquid - Google Patents
A kind of preparation method and applications of fermented ginseng liquid Download PDFInfo
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- CN107137286A CN107137286A CN201710428845.4A CN201710428845A CN107137286A CN 107137286 A CN107137286 A CN 107137286A CN 201710428845 A CN201710428845 A CN 201710428845A CN 107137286 A CN107137286 A CN 107137286A
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- ginseng
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- kefir grains
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- 235000008434 ginseng Nutrition 0.000 title claims abstract description 118
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 117
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 117
- 239000007788 liquid Substances 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 241000208340 Araliaceae Species 0.000 title claims abstract 15
- 241000894006 Bacteria Species 0.000 claims abstract description 54
- 235000015141 kefir Nutrition 0.000 claims abstract description 48
- 235000020710 ginseng extract Nutrition 0.000 claims abstract description 34
- 229930091371 Fructose Natural products 0.000 claims abstract description 15
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 15
- 239000005715 Fructose Substances 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 9
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims abstract 3
- 238000000855 fermentation Methods 0.000 claims description 25
- 230000004151 fermentation Effects 0.000 claims description 25
- 230000001954 sterilising effect Effects 0.000 claims description 25
- 238000007598 dipping method Methods 0.000 claims description 17
- 239000002537 cosmetic Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 235000013336 milk Nutrition 0.000 claims description 9
- 239000008267 milk Substances 0.000 claims description 9
- 210000004080 milk Anatomy 0.000 claims description 9
- 241001465754 Metazoa Species 0.000 claims description 8
- 210000000481 breast Anatomy 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 6
- 235000020251 goat milk Nutrition 0.000 claims description 5
- 235000020248 camel milk Nutrition 0.000 claims description 4
- 238000005470 impregnation Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 39
- 239000002994 raw material Substances 0.000 abstract description 36
- 238000000034 method Methods 0.000 abstract description 20
- 230000003020 moisturizing effect Effects 0.000 abstract description 12
- 239000012530 fluid Substances 0.000 abstract description 7
- 230000003078 antioxidant effect Effects 0.000 abstract description 5
- 229930182494 ginsenoside Natural products 0.000 abstract description 4
- 229940089161 ginsenoside Drugs 0.000 abstract description 4
- 125000003118 aryl group Chemical group 0.000 abstract description 3
- 239000000796 flavoring agent Substances 0.000 abstract description 3
- 235000019634 flavors Nutrition 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 3
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 240000004371 Panax ginseng Species 0.000 description 103
- 239000000047 product Substances 0.000 description 36
- 238000005516 engineering process Methods 0.000 description 17
- BWKOZPVPARTQIV-UHFFFAOYSA-N azanium;hydron;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [NH4+].OC(=O)CC(O)(C(O)=O)CC([O-])=O BWKOZPVPARTQIV-UHFFFAOYSA-N 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 239000000706 filtrate Substances 0.000 description 9
- 238000000605 extraction Methods 0.000 description 7
- 235000011187 glycerol Nutrition 0.000 description 6
- 210000004700 fetal blood Anatomy 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
- 235000019219 chocolate Nutrition 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 238000002803 maceration Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 3
- 238000000205 computational method Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000012805 post-processing Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000415246 Callisia navicularis Species 0.000 description 1
- 235000002791 Panax Nutrition 0.000 description 1
- 241000208343 Panax Species 0.000 description 1
- 235000002789 Panax ginseng Nutrition 0.000 description 1
- 240000007263 Pinus koraiensis Species 0.000 description 1
- 235000011615 Pinus koraiensis Nutrition 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 230000006538 anaerobic glycolysis Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003026 anti-oxygenic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Emergency Medicine (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a kind of preparation method of fermented ginseng liquid, comprise the following steps, after first mixing Ginseng extract, fructose and ammonium citrate, obtain mixed liquor；Then Kefir grains bacterium is inoculated with the mixed liquor that above-mentioned steps are obtained, is fermented, obtains fermented ginseng liquid.The present invention produces the various active compositions such as ginsenoside, prepares the zymotic fluid with moisturizing and antioxidant effect by biofermentation means；And the method provided all makes full use of fermented ginseng liquid, the activity and nutritional ingredient of ginseng are effectively released, technique is simple, and cost is low, can realize large-scale production；The raw material sources of fermented ginseng liquid extensively, obtain product aromatic flavour, with good anti-moisturizing and anti-oxidation efficacy simultaneously.
Description
Technical field
The present invention relates to cosmetics technical field, it is related to a kind of preparation method and applications of fermented ginseng liquid, especially
It is related to a kind of preparation method and applications of the fermented ginseng liquid with moisture-keeping efficacy.
Background technology
Ginseng (Panax ginseng C.A.Mey), also referred to as yellow ginseng, smart, god is careless or kings of hundred grass, be Umbellales five
Plus section Panax perennial vertical herbage plant, happiness cool place, blade pore-free and palisade tissue, it is impossible to retain moisture, temperature is high
It can be burnt in 32 degree of blades, canopy density 0.7~0.8 are bloomed for usual 3 years, 5~6 annual bearings, 5~June of florescence, fruiting period June to September,
The theropencedrymion based on Korean pine for hundreds of meters of height above sea level being grown between 33 °~48 ° of north latitude or broad leaved and deciduous broad leaved sylvan life, are originated in
Northeast China, Korea, South Korea, Japan, Russian east.
In China, ginseng is always considered as the king of hundred grass, is described as the pole of " kings of hundred grass " " life is mended in enriching yin, is strengthened the body resistance to consolidate the constitution "
Product, ginseng contains a variety of saponins and polysaccharide constituents, and with multiple efficacies, its Central nervous system has excitation, and big
There is inhibitory action during amount on the contrary, and adaptability of the body to all nonspecific stimulations can be strengthened, sense of fatigue can be reduced；May be used also
To prevent and treat acute, the chronic plateau sickness of anoxic, tissue respiration can be strengthened, promote anaerobic glycolysis, production is improved under anoxic conditions
Can, body oxygen consumption is reduced, so neuron and cardiovascular system can be protected to improve the tolerance to anoxic, moreover it is possible to improve brain
Oxygen uptake capacity；With antifatigue effect；The adaptability of body can be improved, changes the reactivity of body；Additionally it is possible to the heart
Vascular system, liver, internal system, metabolism etc. are respectively provided with preferable influence, and strengthen body adverse factor is supported
Drag.Thus, the applicating and exploitation on ginseng is always study hotspot in field.
With a long history using Chinese herbal medicine progress cosmetology, ginseng contains the various actives such as ginsenoside, polysaccharide due to it
Material, in addition to the application on medicine, health food, also has application in terms of beauty, and it has increase skin-nourishing, increased
The functions such as strong skin elasticity, the reducing property for suppressing melanin.However as the improvement of people's living standards, moistened for skin
Requirement be not limited to basic cosmetology function, but focus more in its security, natural sex.
Thus, how to improve the cosmetic result of ginseng cosmetics, it has also become many developing enterprises and a line are ground in the industry
Study carefully one of focus of personnel's common concern.
The content of the invention
In view of this, the technical problem to be solved in the present invention be the provision of a kind of fermented ginseng liquid preparation method and its
Using, the preparation method and applications of particularly a kind of fermented ginseng liquid with moisture-keeping efficacy, present invention employs distinctive
Preparation method and formula, obtain fermented ginseng liquid, and skin surface is applied to as cosmetics and skincare product, can play good to skin
Good moistening effect.And preparation method is simple, process control is advantageously implemented scale industrialization.
The invention provides a kind of preparation method of fermented ginseng liquid, comprise the following steps：
1) after mixing Ginseng extract, fructose and ammonium citrate, mixed liquor is obtained；
2) Kefir grains bacterium is inoculated with the mixed liquor that above-mentioned steps are obtained, is fermented, obtains fermented ginseng liquid.
It is preferred that, the mass ratio of the Ginseng extract and the fructose is 100：(5~10)；
The mass ratio of the Ginseng extract and the ammonium citrate is 100：(2~3).
It is preferred that, sterilization steps are also included after the mixing；
The temperature of the sterilizing is 110~130 DEG C；The time of the sterilizing is 10~30min.
It is preferred that, the fermented ginseng liquid is prepared by following steps：
After ginseng is impregnated in water, then concentrated, obtain Ginseng extract.
It is preferred that, the ginseng includes ginseng pulverate；
The mass ratio of the ginseng and water is 1：(25~35)；
The dipping includes single impregnation or repeatedly dipping；
The volume concentration ratio of the concentration is (5~15)：1.
It is preferred that, the granularity of the ginseng pulverate is 40~80 mesh；
The temperature of the dipping is 75~85 DEG C；The time of the dipping is 1.5~2.5h；
The number of times repeatedly impregnated is 2~5 times.
It is preferred that, it is described to be inoculated with concretely comprising the following steps for Kefir grains bacterium：
Kefir grains bacterium is cultivated in animal breast, Kefir grains bacterium seed liquor is obtained；
Then described Kefir grains bacterium seed liquor is added in the mixed liquor, completes the inoculation of Kefir grains bacterium.
It is preferred that, the animal breast includes the one or more in milk, goat milk, mare's milk and camel milk；
Viable bacteria amount in the Kefir grains bacterium seed liquor is 3 × 1010~9 × 1010cfu/ml；
The volume ratio that the volume of the Kefir grains bacterium seed liquor accounts for the mixed liquor is 10%~15%；
The temperature of the culture is 30~40 DEG C；The time of the culture is 0.5~2 day.
It is preferred that, the fermentation is shaker fermentation；
The rotating speed of the shaker fermentation is 150~170r/min；
The temperature of the fermentation is 30~35 DEG C；The time of the fermentation is 35~45h.
The invention provides application of the fermented ginseng liquid in cosmetics prepared by above-mentioned technical proposal any one.
The invention provides a kind of preparation method of fermented ginseng liquid, comprise the following steps, first by Ginseng extract, fruit
After sugar and ammonium citrate mixing, mixed liquor is obtained；Then Kefir grains bacterium is inoculated with the mixed liquor that above-mentioned steps are obtained, is sent out
Ferment, obtains fermented ginseng liquid.Compared with prior art, the present invention for existing ginseng cosmetics skin moisten on not
Foot, creative use Kefir grains bacterium is fermented to Ginseng extract, obtains the fermented ginseng liquid with outstanding moistening effect.
The present invention is produced the various active compositions such as ginsenoside, prepared with moisturizing and antioxidant effect by biofermentation means
Zymotic fluid；And the method provided all makes full use of fermented ginseng liquid, technique is simple, and cost is low, can realize big rule
Mould is produced；The raw material sources of fermented ginseng liquid extensively, obtain product aromatic flavour simultaneously, with good anti-moisturizing and anti-oxidant
Effect.Present invention employs distinctive preparation method and specific raw material, fermented ginseng liquid is obtained, so as to effectively release
The activity and nutritional ingredient of ginseng, and then the fermented ginseng liquid for obtaining fermentation has outstanding moistening effect, is protected as beauty
Skin product are applied to skin surface, and good moistening effect can be played to skin.
Test result indicates that, fermented ginseng liquid prepared by the present invention is applied to skin surface, and its moistening effect is better than
10% glycerine.
Brief description of the drawings
Fig. 1 is the moisturizing rate column pair of fermented ginseng liquid, 10% glycerine and positive controls prepared by the embodiment of the present invention 1
Than figure.
Embodiment
In order to further appreciate that the present invention, the preferred embodiments of the invention are described with reference to embodiment, but
It is it should be appreciated that these descriptions are simply to further illustrate the features and advantages of the present invention rather than to patent requirements of the present invention
Limitation.
All raw materials of the present invention, originate to it and are not particularly limited, commercially buying or according to people in the art
Known to member prepared by conventional method.
All raw materials of the present invention, its purity are not particularly limited, present invention preferably employs biological field or cosmetics
The conventional purity in field.
All raw materials of the present invention are as being all the raw material after sterilizing based on the basic general knowledge in this area without especially indicating.
The invention provides a kind of preparation method of fermented ginseng liquid, comprise the following steps：
1) after mixing Ginseng extract, fructose and ammonium citrate, mixed liquor is obtained；
2) Kefir grains bacterium is inoculated with the mixed liquor that above-mentioned steps are obtained, is fermented, obtains fermented ginseng liquid.
After the present invention first mixes Ginseng extract, fructose and ammonium citrate, mixed liquor is obtained.
The consumption of the fructose is not particularly limited the present invention, is with conventional amount used well known to those skilled in the art
Can, those skilled in the art can be selected and be adjusted, this hair according to practical situations, raw material condition and product requirement
The bright moistening effect to ensure and improving fermented ginseng liquid, the mass ratio of the Ginseng extract and the fructose is preferably 100：
(5~10), more preferably 100：(6~9), most preferably 100：(7~8).
The consumption of the ammonium citrate is not particularly limited the present invention, with conventional amount used well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical situations, raw material condition and product requirement, this
Invent to ensure and improving the moistening effect of fermented ginseng liquid, the mass ratio of the Ginseng extract and the ammonium citrate is preferred
For 100：(2~3), more preferably 100：(2.2~2.8), most preferably 100：(2.4~2.6).
The source of the fermented ginseng liquid is not particularly limited the present invention, is come with routine well known to those skilled in the art
Source, can buy from it is commercially available or according to conventional method prepare, those skilled in the art can be according to practical situations, original
Material situation and product requirement are selected and adjusted, and the present invention is described to ensure and improving the moistening effect of fermented ginseng liquid
Fermented ginseng liquid is prepared by following steps：
After ginseng is impregnated in water, then concentrated, obtain Ginseng extract.
After the present invention soaks ginseng in water, maceration extract is obtained, then maceration extract is concentrated, obtain ginseng extraction
Liquid.
The specifically chosen of the ginseng is not particularly limited the present invention, with common people well known to those skilled in the art
Join species, can buy from it is commercially available or according to conventional method prepare, those skilled in the art can be according to practical application feelings
Condition, raw material condition and product requirement are selected and adjusted.The quality of the ginseng is not particularly limited the present invention, with this
Common ginseng quality known to art personnel, can buy from it is commercially available or according to conventional method prepare, this area
Technical staff can be selected and be adjusted according to practical situations, raw material condition and product requirement, people of the present invention
Ginseng is preferably ginseng pulverate.The granularity of ginseng pulverate of the present invention is preferably 40~80 mesh, more preferably 50~70 mesh, is most preferably
55~65 mesh.
The ratio of the ginseng and water is not particularly limited the present invention, with conventional ratio well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
Invent to ensure and improving the moistening effect of fermented ginseng liquid, the mass ratio of the ginseng and water is preferably 1：(25~35), more
Preferably 1：(27~33), most preferably 1：(29~31).
The mode of the dipping is not particularly limited the present invention, with conventional impregnation method well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
The invention dipping is preferably to soak, and is preferably also impregnated under conditions of stirring, more preferably single impregnation or repeatedly dipping,
Most preferably repeatedly impregnate.The specific number of times of the present invention repeatedly impregnated is preferably 2~5 times, more preferably 3~4 times.
The parameter of the dipping is not particularly limited the present invention, with conventional dip parameters well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
Invent to ensure and improving the moistening effect of fermented ginseng liquid, the temperature of the dipping is preferably 75~85 DEG C, more preferably 77
~83 DEG C, most preferably 79~81 DEG C.The time of dipping of the present invention is preferably 1.5~2.5h, more preferably 1.7~
2.3h, most preferably 1.9~2.1h.
The parameter of the concentration is not particularly limited the present invention, with Conventional concentration parameter well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
The volume concentration ratio of the concentration is invented, i.e., the volume ratio of described maceration extract and Ginseng extract is preferably (5~15):1, more
Preferably (7~13)：1, be most preferably (9~11)：1.
The present invention is to improve the leaching content of active component in ginseng, it is ensured that and improve the effect of fermented ginseng liquid, above-mentioned step
Rapid is more specifically preferably following steps：
Preferably repeatedly impregnated under agitation in the present invention, will impregnate obtained ginseng residue's progress for the first time first
Second of dipping, second impregnates obtained ginseng residue and carries out third time dipping, and by that analogy, finally merging impregnates obtain every time
Maceration extract concentrated, obtain Ginseng extract.
In the present invention, the mass ratio of ginseng and water is preferably the mass ratio in each dipping process.
The mode of the mixing is not particularly limited the present invention, with conventional hybrid mode well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
Invent to ensure and improve the effect of fermented ginseng liquid and the integrality of technology chain, sterilizing step is preferably included after the mixing
Suddenly.
The concrete mode and parameter of the sterilization steps are not particularly limited the present invention, are known with those skilled in the art
Conventional sterilant mode and parameter, those skilled in the art can be according to practical condition, raw material condition and product
It is required that being selected and being adjusted, the present invention is described to ensure and improving the effect of fermented ginseng liquid and the integrality of technology chain
The temperature of sterilizing is preferably 110~130 DEG C, more preferably 115~125 DEG C, most preferably 117~123 DEG C.The sterilizing when
Between be preferably 10~30min, most preferably more preferably 15~25min, 18~23min.
The present invention passes through above-mentioned steps, has obtained sterilizing mixed liquor, is then inoculated with the mixed liquor that above-mentioned steps are obtained
Kefir grains bacterium, then fermented, obtain fermented ginseng liquid.
The source of the Kefir grains bacterium is not particularly limited the present invention, is opened with well known to those skilled in the art conventional
The source of Fei Er bacterium, can buy from commercially available, those skilled in the art can according to practical condition, raw material condition with
And product requirement is selected and adjusted, the preferred purchase of Kefir grains bacterium of the present invention is from commercially available, more preferably through overactivation
Commercially available Kefir grains bacterium.The mode and parameter of the activation are not particularly limited the present invention, with well known to those skilled in the art
The activation method and parameter of conventional Kefir grains bacterium, those skilled in the art can be according to practical condition, raw material feelings
Condition and product requirement are selected and adjusted.
The concrete mode and parameter of the inoculation are not particularly limited the present invention, with well known to those skilled in the art normal
The vaccination ways and parameter of rule, those skilled in the art can want according to practical condition, raw material condition and product
Ask and selected and adjusted, the present invention is described to connect to ensure and improving the effect of fermented ginseng liquid and the integrality of technology chain
Plant concretely comprising the following steps for Kefir grains bacterium：
Kefir grains bacterium is cultivated in animal breast, Kefir grains bacterium seed liquor is obtained；
Then described Kefir grains bacterium seed liquor is added in the mixed liquor, completes the inoculation of Kefir grains bacterium.
The animal breast is not particularly limited the present invention, is used to cultivate Kefir grains bacterium with well known to those skilled in the art
Animal breast, can buy from commercially available or voluntarily extract, those skilled in the art can be according to practical condition, raw material
Situation and product requirement are selected, and animal breast of the present invention preferably includes one in milk, goat milk, mare's milk and camel milk
Plant or a variety of, more preferably milk, goat milk or camel milk, more preferably milk or goat milk, most preferably milk, be specifically as follows
Plain chocolate.
The temperature of the culture is not particularly limited the present invention, with conventional such bacterium well known to those skilled in the art
The cultivation temperature planted, those skilled in the art can be carried out according to practical condition, raw material condition and product requirement
Selection and adjustment, the present invention are the integrality for the effect and technology chain for ensureing and improving fermented ginseng liquid, the temperature of the culture
Degree is preferably 30~40 DEG C, most preferably more preferably 32~38 DEG C, 34~36 DEG C.
The time of the culture is not particularly limited the present invention, with conventional such bacterium well known to those skilled in the art
The incubation time planted, those skilled in the art can be carried out according to practical condition, raw material condition and product requirement
Selection and adjust, the present invention for ensure and improve fermented ginseng liquid effect and technology chain integrality, the culture when
Between be preferably 0.5~2 day, more preferably 1~1.5 day.
The parameter of the Kefir grains bacterium seed liquor is not particularly limited the present invention, and those skilled in the art can be according to reality
The border condition of production, raw material condition and product requirement are selected and adjusted, and the present invention is guarantee and improves fermented ginseng liquid
Viable bacteria amount in effect, the Kefir grains bacterium seed liquor is preferably 3 × 1010~9 × 1010Cfu/ml, more preferably 4 × 1010~
8×1010Cfu/ml, more preferably 5 × 1010~7 × 1010Cfu/ml, is specifically as follows 6 × 1010cfu/ml。
The computational methods of the viable bacteria amount of the Kefir grains bacterium seed liquor are not particularly limited the present invention, with art technology
The conventional computational methods of such mushroom known to personnel, those skilled in the art can be according to practical condition, original
Material situation and product requirement are selected and adjusted, the computational methods of the viable bacteria amount in Kefir grains bacterium seed liquor of the present invention
Preferably viable bacteria counting method or OD value determination methods, more preferably OD values determination method, more specifically preferably determine and adjust according to OD values
To proper range.
The ratio of the Kefir grains bacterium seed liquor and mixed liquor is not particularly limited the present invention, with those skilled in the art
Well known conventional ratio, those skilled in the art can enter according to practical condition, raw material condition and product requirement
Row selection and adjustment, the present invention are the integrality for the effect and technology chain for ensureing and improving fermented ginseng liquid, the Kefir grains
The volume ratio that the volume of bacterium seed liquor accounts for the mixed liquor is preferably 10%~15%, and more preferably 11%~14%, most preferably
For 12%~13%.
The last mixed liquor by completion Kefir grains bacterium inoculation of the invention is fermented, and obtains fermented ginseng liquid.
The mode of the fermentation is not particularly limited the present invention, is with usual manner well known to those skilled in the art
Can, those skilled in the art can be selected and be adjusted, this hair according to practical condition, raw material condition and product requirement
The bright integrality to ensure and improving the effect and technology chain of fermented ginseng liquid, the mode of the fermentation is preferably shaking table hair
Ferment, i.e., preferably fermented in shaking table.
The parameter of the shaker fermentation is not particularly limited the present invention, with conventional parameter well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
Invent to ensure and improving the effect of fermented ginseng liquid and the integrality of technology chain, the rotating speed of the shaker fermentation is preferably
150~170r/min, more preferably 155~165r/min, most preferably 157~163r/min.
The temperature of the fermentation is not particularly limited the present invention, with normal fermentation temperature well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
Invent to ensure and improving the effect of fermented ginseng liquid and the integrality of technology chain, the temperature of the fermentation is preferably 30~35
DEG C, more preferably 31~34 DEG C, most preferably 32~33 DEG C.
The time of the fermentation is not particularly limited the present invention, with the normal fermentation time well known to those skilled in the art
, those skilled in the art can be selected and be adjusted according to practical condition, raw material condition and product requirement, this
To invent to ensure and improve the effect of fermented ginseng liquid and the integrality of technology chain, time of the fermentation is preferably 35~
45h, more preferably 37~43h, most preferably 39~41h.
The present invention is ensures and improved the effect of fermented ginseng liquid and the integrality of technology chain, after the fermentation preferably also
Including post-processing step.
The detailed process of the post processing is not particularly limited the present invention, after routine well known to those skilled in the art
Processing procedure, those skilled in the art can be selected according to practical condition, raw material condition and product requirement
And adjustment, post processing of the present invention preferably includes the one or more in separating, sterilize and preserving, and is more preferably followed successively by point
From, sterilization and preserve step.
The concrete mode and parameter of the separation are not particularly limited for invention, with routine well known to those skilled in the art
Separate mode and parametric procedure, those skilled in the art can want according to practical condition, raw material condition and product
Ask and selected and adjusted, separation of the present invention is preferably to centrifuge.The rotating speed of centrifugation of the present invention is preferably
4000~6000r/min, more preferably 4500~5500r/min, most preferably 4700~5300r/min.It is of the present invention from
The time of heart separation is preferably 10~15min, more preferably 11~14min, most preferably 12~13min.
The present invention preferably wraps to ensure and improving the effect of fermented ginseng liquid and the integrality of technology chain after the separation
Sterilization steps are included, more preferably including sterilizing and Cord blood step, are specifically as follows heat sterilization and Cord blood step.
The concrete mode and parameter of the sterilization steps are not particularly limited the present invention, are known with those skilled in the art
Conventional sterilant mode and parameter, those skilled in the art can be according to practical condition, raw material condition and product
It is required that being selected and being adjusted, the present invention is described to ensure and improving the effect of fermented ginseng liquid and the integrality of technology chain
The temperature of sterilizing is preferably 60~70 DEG C, more preferably 62~68 DEG C, most preferably 64~66 DEG C.The time of the sterilizing is preferred
For 30~40min, most preferably more preferably 32~38min, 34~36min.
The concrete mode and parameter of the Cord blood step are not particularly limited the present invention, with those skilled in the art
Well known conventional Cord blood mode and parameter, those skilled in the art can be according to practical condition, raw material feelings
Condition and product requirement are selected and adjusted, and the present invention is complete for the effect and technology chain of guarantee and raising fermented ginseng liquid
Whole property, the temperature of the Cord blood is preferably 1~6 DEG C, more preferably 2~5 DEG C, most preferably 3~4 DEG C.
Present invention also offers the answering in cosmetics of the fermented ginseng liquid prepared by above-mentioned technical proposal any one
With.
The cosmetics are not particularly limited the present invention, are with conventional cosmetic product well known to those skilled in the art
Can, those skilled in the art can be selected and be adjusted, this hair according to practical situations, raw material condition and product requirement
The bright cosmetics preferably have or protruded the cosmetics of moistening effect or antioxygenic property.
Above-mentioned steps of the present invention provide a kind of preparation method and applications of fermented ginseng liquid, and the invention is adopted
Ginseng extract is fermented with Kefir grains bacterium, the fermented ginseng liquid with outstanding moistening effect is obtained.The present invention passes through life
Thing fermentation means, produce the various active compositions such as ginsenoside, in conjunction with specific component and preparation technology, have obtained having and have protected
The zymotic fluid of wet and antioxidant effect；And the method provided all makes full use of fermented ginseng liquid, technique is simple, cost
It is low, large-scale production can be realized；The raw material sources of fermented ginseng liquid extensively, obtain product aromatic flavour, with good simultaneously
Anti- moisturizing and anti-oxidation efficacy.Further, the condition of zymotechnique is optimized the present invention, not only increases fermentation
Speed, shortens fermentation time, and the moisturizing of zymotic fluid and it is anti-oxidant the effects such as be significantly improved.Present invention employs
Distinctive preparation method and specific raw material, obtain fermented ginseng liquid, and skin surface is applied to as cosmetics and skincare product, can be with
Good moistening effect is played to skin.
Test result indicates that, the moistening effect of fermented ginseng liquid prepared by the present invention is better than 10% glycerine.
In order to further illustrate the present invention, a kind of preparation of the fermented ginseng liquid provided with reference to embodiments the present invention
Method and its application are described in detail, but it is to be understood that these embodiments are under premised on technical solution of the present invention
Implemented, give detailed embodiment and specific operating process, simply for further illustrate the present invention feature and
Advantage, rather than limiting to the claimed invention, protection scope of the present invention are also not necessarily limited to following embodiments.
Embodiment 1
(1) first dry ginseng is crushed, crosses 40 mesh sieves.
(2) extraction is stirred to the ginseng after crushing at 80 DEG C with the water of 20 times of weight again, extraction time is 2h, mistake
Filter obtains filtrate；Extract 2 times repeatedly, merging filtrate；The 10% of original volume is concentrated the filtrate to, Ginseng extract is obtained.
(3) the Kefir grains strain activated a ring (strain source is in Agricultural University Of South China's natural active products research center)
It is added in plain chocolate and cultivates, be uniformly dissolved, 120 DEG C of sterilizing 20min is cooled to after room temperature and are inoculated with.It is 30 DEG C of conditions in temperature
Lower culture 1 day, obtains Kefir grains bacterium seed liquor.
(4) fructose, ammonium citrate are added into Ginseng extract, fructose addition is the 7% of Ginseng extract, citric acid
Ammonium addition is the 3% of Ginseng extract, is then mixed, 120 DEG C of sterilizing 20min, is cooled to after room temperature, aseptically,
Kefir grains bacterium seed liquor is added, addition is the 10% of Ginseng extract, ferments 1 day, obtains in 25 DEG C on 150r/min shaking tables
To tunning.
(5) tunning is taken out, imported in centrifuge tube, 4000r/min centrifugation 10min abandon lower sediment, obtain ginseng
Zymotic fluid；The fermented ginseng liquid is placed in 65 DEG C of water-baths and sterilizes 30min, after cooling, and 4 DEG C of refrigerators are preserved.
Embodiment 2
(1) first dry ginseng is crushed, crosses 40 mesh sieves.
(2) extraction is stirred to the ginseng after crushing at 75 DEG C with the water of 15 times of weight again, extraction time is 2h, mistake
Filter obtains filtrate；Extract 2 times repeatedly, merging filtrate；The 10% of original volume is concentrated the filtrate to, Ginseng extract is obtained.
(3) the Kefir grains strain activated a ring (strain source is in Agricultural University Of South China's natural active products research center)
It is added in plain chocolate and cultivates, be uniformly dissolved, 121 DEG C of sterilizing 20min is cooled to after room temperature and are inoculated with.It is 30 DEG C of conditions in temperature
Lower culture 1 day, obtains Kefir grains bacterium seed liquor.
(4) fructose, ammonium citrate are added into Ginseng extract, fructose addition is the 6% of Ginseng extract, citric acid
Ammonium addition is the 2% of Ginseng extract, is then mixed, 121 DEG C of sterilizing 20min, is cooled to after room temperature, aseptically,
Kefir grains bacterium seed liquor is added, addition is the 10% of Ginseng extract, ferments 1 day, obtains in 24 DEG C on 150r/min shaking tables
To tunning.
(5) tunning is taken out, imported in centrifuge tube, 4000r/min centrifugation 10min abandon lower sediment, obtain ginseng
Zymotic fluid；The fermented ginseng liquid is placed in 65 DEG C of water-baths and sterilizes 30min, after cooling, and 4 DEG C of refrigerators are preserved.
Embodiment 3
(1) first dry ginseng is crushed, crosses 40 mesh sieves.
(2) extraction is stirred to the ginseng after crushing at 85 DEG C with the water of 25 times of weight again, extraction time is 2h, mistake
Filter obtains filtrate；Extract 2 times repeatedly, merging filtrate；The 10% of original volume is concentrated the filtrate to, Ginseng extract is obtained.
(3) the Kefir grains strain activated a ring (strain source is in Agricultural University Of South China's natural active products research center)
It is added in plain chocolate and cultivates, be uniformly dissolved, 119 DEG C of sterilizing 20min is cooled to after room temperature and are inoculated with.It is 31 DEG C of conditions in temperature
Lower culture 1 day, obtains Kefir grains bacterium seed liquor.
(4) fructose, ammonium citrate are added into Ginseng extract, fructose addition is the 8% of Ginseng extract, citric acid
Ammonium addition is the 4% of Ginseng extract, is then mixed, 119 DEG C of sterilizing 20min, is cooled to after room temperature, aseptically,
Kefir grains bacterium seed liquor is added, addition is the 10% of Ginseng extract, ferments 1 day, obtains in 26 DEG C on 150r/min shaking tables
To tunning.
(5) tunning is taken out, imported in centrifuge tube, 4000r/min centrifugation 10min abandon lower sediment, obtain ginseng
Zymotic fluid；The fermented ginseng liquid is placed in 65 DEG C of water-baths and sterilizes 30min, after cooling, and 4 DEG C of refrigerators are preserved.
Embodiment 4
Fermented ginseng liquid prepared by the embodiment of the present invention 1 is detected.
The moistening effect evaluation of fermented ginseng liquid.
Moistening effect evaluation method：3M adhesive tapes are sticked in glass plate (50 × 100mm), take 200mg samples uniformly to smear
In tape surface, put into drier, humid control is 60% or so in drier.Weighed after putting drier certain time into
(time interval is 0.5h, 1h, 2h, 4h, 8h), calculates moisturizing rate, moisturizing rate=M2÷M1× 100%, wherein, M2After placement
Moisture weight, M1For the moisture weight before placement.
It is (positive to obtained fermented ginseng liquid, 10% glycerine and positive controls in embodiment 1 respectively according to the method described above
Control group selects commercially available A facial treatment essence, and commercially available A facial treatment essence contains zymasis, with moisturizing, moisturizing and anti-ageing work(
Effect) moisturizing rate calculated.Referring to Fig. 1, Fig. 1 is fermented ginseng liquid, 10% glycerine and the positive prepared by the embodiment of the present invention 1
The moisturizing rate column comparison diagram of control group.As shown in Figure 1, the moistening effect for the fermented ginseng liquid that the present invention is prepared is better than
10% glycerine, and in 1h, 2h, 8h, the moistening effect for the fermented ginseng liquid that the present invention is prepared is better than positive controls.
A kind of preparation method and applications of the fermented ginseng liquid with moisture-keeping efficacy provided above the present invention are carried out
Detailed introduction, specific case used herein is set forth to the principle and embodiment of the present invention, and the above is implemented
The explanation of example is only intended to the method and its core concept for helping to understand the present invention, including best mode, and also causes ability
Any technical staff in domain can put into practice the present invention, including manufacture and use any device or system, and implement any combination
Method.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention,
Some improvement and modification can also be carried out to the present invention, these are improved and modification also falls into the protection domain of the claims in the present invention
It is interior.The scope of patent protection of the present invention is defined by the claims, and may include those skilled in the art it is conceivable that its
His embodiment.If these other embodiments are with the structural element for being not different from claim character express, or if
They include equivalent structural elements of the character express without essence difference with claim, then these other embodiments should also be wrapped
Containing within the scope of the claims.
Claims (10)
1. a kind of preparation method of fermented ginseng liquid, it is characterised in that comprise the following steps：
1) after mixing Ginseng extract, fructose and ammonium citrate, mixed liquor is obtained；
2) Kefir grains bacterium is inoculated with the mixed liquor that above-mentioned steps are obtained, is fermented, obtains fermented ginseng liquid.
2. preparation method according to claim 1, it is characterised in that the mass ratio of the Ginseng extract and the fructose
For 100：(5~10)；
The mass ratio of the Ginseng extract and the ammonium citrate is 100：(2~3).
3. preparation method according to claim 1, it is characterised in that also include sterilization steps after the mixing；
The temperature of the sterilizing is 110~130 DEG C；The time of the sterilizing is 10~30min.
4. preparation method according to claim 1, it is characterised in that the fermented ginseng liquid is prepared into by following steps
Arrive：
After ginseng is impregnated in water, then concentrated, obtain Ginseng extract.
5. preparation method according to claim 4, it is characterised in that the ginseng includes ginseng pulverate；
The mass ratio of the ginseng and water is 1：(25~35)；
The dipping includes single impregnation or repeatedly dipping；
The volume concentration ratio of the concentration is (5~15)：1.
6. preparation method according to claim 5, it is characterised in that the granularity of the ginseng pulverate is 40~80 mesh；
The temperature of the dipping is 75~85 DEG C；The time of the dipping is 1.5~2.5h；
The number of times repeatedly impregnated is 2~5 times.
7. preparation method according to claim 1, it is characterised in that the inoculation Kefir grains bacterium concretely comprises the following steps：
Kefir grains bacterium is cultivated in animal breast, Kefir grains bacterium seed liquor is obtained；
Then described Kefir grains bacterium seed liquor is added in the mixed liquor, completes the inoculation of Kefir grains bacterium.
8. preparation method according to claim 7, it is characterised in that
The animal breast includes the one or more in milk, goat milk, mare's milk and camel milk；
Viable bacteria amount in the Kefir grains bacterium seed liquor is 3 × 1010~9 × 1010cfu/ml；
The volume ratio that the volume of the Kefir grains bacterium seed liquor accounts for the mixed liquor is 10%~15%；
The temperature of the culture is 30~40 DEG C；The time of the culture is 0.5~2 day.
9. preparation method according to claim 1, it is characterised in that the fermentation is shaker fermentation；
The rotating speed of the shaker fermentation is 150~170r/min；
The temperature of the fermentation is 30~35 DEG C；The time of the fermentation is 35~45h.
10. application of the fermented ginseng liquid in cosmetics prepared by claim 1~9 any one.
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| CN104825515A (en) * | 2015-05-15 | 2015-08-12 | 吉林农业大学 | Kefir particle fermented ginseng powder |
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