CN107125169A - A kind of temperature adjusting method of silvery pomfret parent reinforced cultivating - Google Patents
A kind of temperature adjusting method of silvery pomfret parent reinforced cultivating Download PDFInfo
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- CN107125169A CN107125169A CN201710344645.0A CN201710344645A CN107125169A CN 107125169 A CN107125169 A CN 107125169A CN 201710344645 A CN201710344645 A CN 201710344645A CN 107125169 A CN107125169 A CN 107125169A
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- Prior art keywords
- silvery pomfret
- parent
- water temperature
- adjusting method
- temperature
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- 241000630524 Taractes rubescens Species 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000004720 fertilization Effects 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000009434 installation Methods 0.000 claims description 2
- 210000004907 gland Anatomy 0.000 abstract description 11
- 238000011534 incubation Methods 0.000 abstract description 11
- 210000004392 genitalia Anatomy 0.000 abstract description 9
- 230000001850 reproductive effect Effects 0.000 abstract description 7
- 230000002710 gonadal effect Effects 0.000 abstract description 5
- 238000009825 accumulation Methods 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 230000000638 stimulation Effects 0.000 description 8
- 241000251468 Actinopterygii Species 0.000 description 7
- 150000002632 lipids Chemical class 0.000 description 7
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 210000004681 ovum Anatomy 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 210000001672 ovary Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 230000026109 gonad development Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of temperature adjusting method of silvery pomfret parent reinforced cultivating, comprise the following steps:Silvery pomfret parents culture water temperature is down to behind 12~13 DEG C of intervals naturally, by heat maintain water temperature 12~13 DEG C one week;According to daily 0.4~0.6 DEG C of increasing degree, water temperature in culture pond is promoted to 18~19 DEG C;Water temperature in culture pond is maintained at 18~19 DEG C of mid or late Aprils for continueing to Second Year, silvery pomfret parent spawning and fertilization.The present invention can effectively facilitate the nutrient accumulation in silvery pomfret parent's gonadal tissue, significantly improve genital gland indices, Fertilized quality and its incubation rate, the final reproductive performance for improving silvery pomfret.
Description
Technical field
The invention belongs to the technical field of silvery pomfret breeding period temperature adjusting, more particularly to a kind of silvery pomfret parent reinforced cultivating
Temperature adjusting method.
Background technology
Silvery pomfret is one of main marine products economic fish of China.In the last few years, China Aquatic Science Research Institute East Sea aquatic products
Research institute is by constantly furtheing investigate exploration, and reproductive characteristic, gonad development rule and the silvery pomfret for having grasped silvery pomfret are artificial
The pattern and method of nursery, and realize the full artificial breeding of silvery pomfret.However, still not yet realizing the propagation in scale of silvery pomfret at present
And cultivation is promoted.Restricting current silvery pomfret large-scale artificial is bred and cultivated the key technology bottleneck promoted and is to be difficult to cultivate to obtain
The silvery pomfret parent of high-quality, causes smart ovum quality defect, and incubation rate and larva and juvenile survival rate are relatively low.High-quality and the sufficient silvery pomfret of quantity
Parent, is the basic premise for realizing the full artificial breeding of silvery pomfret scale, is also the basic guarantee for obtaining high-quality seed.Fortification
It is no doubt the necessary factor for obtaining high-quality silvery pomfret parent to cultivate, but the Optimum Regulation of ecological factor is also the pass for obtaining Quality parents
Key step.Accordingly, it would be desirable to a kind of ecological factor (temperature) the regulation and control method of silvery pomfret parent reinforced cultivating, to improve its parent's matter
Amount, improves reproductive performance.
The conventional parent temperature adjusting method of fish is that a stable temperature is remained during parents culture mostly,
There is not yet cultivating the mode of operation that early stage carries out moderate low temperature stimulation in constant temperature.The low temperature stimulation of appropriateness can effectively improve fish pair
The tissue deposit of nutriment, so as to finally lift its reproductive performance.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of temperature adjusting method of silvery pomfret parent reinforced cultivating, effectively
Promote the nutrient accumulation in silvery pomfret parent's gonadal tissue, significantly improve genital gland indices, Fertilized quality and its incubation rate, finally
Improve the reproductive performance of silvery pomfret.
The technical solution adopted for the present invention to solve the technical problems is to provide a kind of temperature of silvery pomfret parent reinforced cultivating
Regulation and control method, comprises the following steps:
(1) silvery pomfret parents culture water temperature is down to behind 12~13 DEG C of intervals naturally, and water temperature is maintained 12~13 by heating
DEG C one week;
(2) according to daily 0.4~0.6 DEG C of increasing degree, water temperature in culture pond is promoted to 18~19 DEG C;
(3) water temperature in culture pond is maintained at 18~19 DEG C of mid or late Aprils for continueing to Second Year, the spawning of silvery pomfret parent by
Essence.
Heated in the step (1) using boiler.
The increasing degree of water temperature 0.5 DEG C of installation daily is heated up in culture pond in the step (2).
Beneficial effect
In the present invention, under the premise of the temperature of ingesting of silvery pomfret substantially is maintained, give silvery pomfret appropriate low temperature stimulation, including from
The process so cooled can lay in mechanism with the nutrition in effectively start silvery pomfret parent tissue, can promote silvery pomfret parent time saving and energy savingly
Lipid nutrition deposit in nutrient accumulation in tissue, particularly gonadal tissue, can significantly improve genital gland indices, fertilization archiblast
Amount and its incubation rate, the final reproductive performance for improving silvery pomfret parent are simple and easy to operate.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.In addition, it is to be understood that after the content of the invention lectured has been read, people in the art
Member can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
A kind of experiment of the temperature adjusting method of silvery pomfret parent reinforced cultivating:
Experimental fish:
1 age of artificial culture female parent fish of pomfret.
Experimental design:
Temperature control takes following 4 kinds of patterns during silvery pomfret parents culture:18-19 DEG C of cultivation;18-19 DEG C of cultivation, early stage
By one 12-13 DEG C of low temperature stimulation stage (one week);15-16 DEG C of cultivation;15-16 DEG C of cultivation, early stage is by a 12-13
DEG C the low temperature stimulation stage (one week).
Design temperature in 4 in pattern is to be naturally cooling to test required temperature, and the treatment group of low temperature stimulation is being maintained
Temperature needed for low temperature rises to experiment after one week according to daily 0.5 DEG C of amplification.
Feeding management:
600 tail silvery pomfret parents (phase of gonad development II) are randomized into 12 20m3Cement pit in, each cultivation group sets 3 groups
Repeat.During raising, continuous charge oxygenation is satiated with food feeds 2 times (09 daily:00 and 17:00), day quantity of exchanged water is 30%.Using
The stabilization of water temperature, salinity 25-28 ‰ are tested in the method guarantee of boiler temperature-increasing.
Analysis indexes:
After experiment terminates, 5 tail fishes are taken from each pond at random, after weighing, sexual gland sample is gathered and weighs, to test and analyze
Lipid content in genital gland indices and gonadal tissue;The parent reached maturity is selected in each pond, the size of its ovum grain is detected;
Each pond yield after fertilization is treated, its embryonated egg and incubation rate is counted.
Data analysis:
The statistical analysis of data is carried out using SPSS19.0 softwares, data are represented with mean+SD, P<0.05 table
It is shown with significant difference.
Experimental result:
Table 1:Different disposal group female silvery pomfret genital gland indices and lipid nutrition accumulation (%)
There is significant difference (P between different Superscript letters expression groups with a line in table 1<0.05).
Table 1 shows female parent fish of pomfret genital gland indices and ovary lipid content change under the conditions for the treatment of of different temperature.Can by table 1
Know, 12-13 DEG C/18-19 DEG C treatment group genital gland indices are significantly higher than other each groups, 18-19 DEG C for the treatment of group genital gland indices are significantly high
In 15-16 DEG C and 12-13 DEG C/15-16 DEG C treatment group, but between 15-16 DEG C and 12-13 DEG C/15-16 DEG C treatment group genital gland indices without
Significant difference.Ovary lipid nutrition content is all remarkably higher than other each groups with 12-13 DEG C/18-19 DEG C treatment group highest,
Though slightly above 15-16 DEG C and 12-13 DEG C/15-16 DEG C treatment group of 18-19 DEG C for the treatment of group ovary lipid content, has no between three groups
Significant difference.
Table 2:Different disposal group silvery pomfret rate of fertilization and incubation rate situation
There is significant difference (P between different Superscript letters expression groups with a line in table 2<0.05).
Table 2 shows the change of silvery pomfret rate of fertilization and incubation rate under the conditions for the treatment of of different temperature.As shown in Table 2, ovum grain diameter with
12-13 DEG C/18-19 DEG C treatment group highest, and it is significantly higher than other three treatment groups.The variation tendency of rate of fertilization is with ovum grain diameter
Variation tendency it is basically identical, 12-13 DEG C/18-19 DEG C treatment group is significantly higher than other three each treatment groups, but other three processing
There was no significant difference between group.12-13 DEG C/18-19 DEG C treatment group incubation rate is significantly higher than at other three treatment groups, 18-19 DEG C
Reason group incubation rate is significantly higher than at 15-16 DEG C and 12-13 DEG C/15-16 DEG C treatment group, but 15-16 DEG C and 12-13 DEG C/15-16 DEG C
Simultaneously there was no significant difference for incubation rate between reason group group.
Experiment conclusion:
18-19 DEG C is cultivated and early stage passes through one 12-13 DEG C of low temperature stimulation stage (one week), and its sexual gland of this group refers to
Lipid content, rate of fertilization and incubation rate are highest in number, gonadal tissue, are secondly 18-19 DEG C of cultivation group, show, before surviving the winter
Phase appropriate low temperature stimulation is favorably improved the reproductive performance of silvery pomfret.
Claims (3)
1. a kind of temperature adjusting method of silvery pomfret parent reinforced cultivating, comprises the following steps:
(1) silvery pomfret parents culture water temperature is down to behind 12~13 DEG C of intervals naturally, and water temperature is maintained at 12~13 DEG C one by heating
Week;
(2) according to daily 0.4~0.6 DEG C of increasing degree, water temperature in culture pond is promoted to 18~19 DEG C;
(3) water temperature in culture pond is maintained at 18~19 DEG C of mid or late Aprils for continueing to Second Year, silvery pomfret parent spawning and fertilization.
2. a kind of temperature adjusting method of silvery pomfret parent reinforced cultivating according to claim 1, it is characterised in that:The step
Suddenly heated in (1) using boiler.
3. a kind of temperature adjusting method of silvery pomfret parent reinforced cultivating according to claim 1, it is characterised in that:The step
Suddenly the increasing degree of water temperature 0.5 DEG C of installation daily is heated up in culture pond in (2).
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103125416A (en) * | 2013-03-25 | 2013-06-05 | 江苏中洋集团股份有限公司 | Ecological factorial breeding method of coilia ectenes parent fish |
CN105918164A (en) * | 2016-04-21 | 2016-09-07 | 中国水产科学研究院东海水产研究所 | Nutrition enrichment breeding method for silver pomfret parents |
-
2017
- 2017-05-16 CN CN201710344645.0A patent/CN107125169A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103125416A (en) * | 2013-03-25 | 2013-06-05 | 江苏中洋集团股份有限公司 | Ecological factorial breeding method of coilia ectenes parent fish |
CN105918164A (en) * | 2016-04-21 | 2016-09-07 | 中国水产科学研究院东海水产研究所 | Nutrition enrichment breeding method for silver pomfret parents |
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Application publication date: 20170905 |
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