CN107119113A - Registration screening-gene marker detection method and its application before Noise Exposure workman hilllock - Google Patents
Registration screening-gene marker detection method and its application before Noise Exposure workman hilllock Download PDFInfo
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Abstract
The haemocyte genetic marker object detecting method of Noise Exposure workman, the haemocyte gene biological mark is：HOTAIR gene rs4759314 pleomorphism sites；The rs4759314 polymorphic positions point gene of the HOTAIR genes is SEQ ID NO:Shown in 1：GCTTGGAAGGGATATAAACAGGCCA[A/G]GCGGATGCAAGTTAATAAAACCTGA.DNA is extracted in the control group workman's haemocyte matched first from noise hearing impairment group workman and age-sex etc., it have detected three sites and demonstrate HOTAIR genes rs4759314, haplotype GGG and other haplotypes and the correlation of noise hearing impairment, in this, as the gene marker of examination before Noise Exposure workman hilllock, it is simple to operate, sensitivity is high, suitable for extensive Susceptible population's examination, foundation is provided for registration screening before Noise Exposure workman hilllock.
Description
Technical field
The invention belongs to fields of biotechnology, it is related to the application of DNA in haemocyte, discloses a kind of for Noise Exposure
Registration is screened before workman hilllock biomarker and its application.
Background technology
NIHL (noise-induced hearing loss, noise induced hearing loss) is also known as occupational noise deaf, is people
It is long-term in the course of the work caused slow, progressive phonosensitive nerve hearing loss is stimulated by noise, be one
Important occupational disease is planted, is also to endanger one of the healthy main occupational disease of labourer (Carlsson, P.I.et al
2005).In the U.S., about 10% work man-hour is exposed in hazardous noise environment；And in Europe about 20%
Workman is exposed in hazardous noise working environment (Dobie R A et al 2008).Chinese ten thousand professional population about more than 1000
In exceeded noise circumstance, wherein there are about 1,000,000 people with different degrees of hearing impairment.Long-term work is made an uproar harmful
Effective safeguard procedures are not taken again in acoustic environment, permanent, irreversible hearing loss will be caused to human body, or even lead
Serious NIHL is caused, therefore reduces its incidence of disease and progressively prevents NIHL generation to be faced as current prevention and control of occupational disease
A unusual arduous and important task.
Noise induced hearing loss is environmental factor and the coefficient result of inherent cause.There are some researches show NIHL is with making an uproar
Acoustic exposure amount is proportionate, and the order of severity of hearing impairment and the length of service of contact noise are also closely related, presents more apparent
When m- dose-effect relationship, i.e. noise be stronger, open-assembly time is more long, then hearing loss more seriously (Prasher D et
al 1998).But this is not to cause NIHL unique influence factor, other environmental impact factors include high temperature, vibration, organic
Solvent etc., goes back has age, sex, smoking, hypertension, takes ototoxic drug (aminoglycoside etc.) individual factors and heredity
Factor etc..Research was shown in same noise environment after the exposure identical time in the past, and the Hearing change of Different Individual has substantially
Difference, some individuals are extremely sensitive to noise, occur NIHL in the short time after Noise Exposure；And another part individual then have compared with
Strong tolerance, remains to keep normal hearing for a long time in noise circumstance, illustrates Different Individual to the susceptible of noise
Property has differences, and thinks that this species diversity is relevant with heredity (Konings A et al 2009).Study NIHL tumor susceptibility gene
Have very important significance to NIHL prevention tool, result of study in recent years displays that some genes related to NIHL neurological susceptibilities change
Become, including chondriogen (mtDNA), presbycusis gene (AHL), the gene of cadherin 23 (CDH23), plasma membrane Ca2+-
The gene of ATP enzyme 2 (PMCA2), heat shock protein 70 (HSP70), also comprising superoxide dismutase (SOD), paraoxonase
(PON), glutathione transferase (GST) isogenic antioxidant system.
HOTAIR genes be located at human chromosomal 12q13, size is about 2158bp, be the long-chain RNA of not encoding proteins i.e.
LncRNA(Chang HY et al 2012).LncRNA HOTAIR (HOX transcript antisense RNA) are so far
It was found that first there is the long-chain non-coding RNA that trans transcriptional control is acted on, be to be found first by Rinn research groups and fixed
Justice, it contains 5 short extrons and 1 long extron, be located on DNA sequence dna the HOXCll genes of No. 12 chromosome with
Between HOXCl2 genes.Numerous researchs show HOTAIR genes unconventionality expression in many cancers, illustrate that HOTAIR is probably
Played an important role in one potential human tumor mark and life process.Kim etc. is on HOTAIR and cancer of pancreas phase
Closing Journal of Sex Research confirms that HOTAIR expressions have been raised in pancreatic cancer cell, with cancer cell invasion positive correlation.In pancreas
HOTAIR is knocked out in cancer cell Pancl, can substantially slow down cancer cell multiplication and change the cell cycle, and cancer cell specific induction of apoptosis.
The experiment of this seminar finds that susceptible group of HOTAIR expressions are higher than control group, points out HOTAIR genes to participate in adjusting cochlea
The cell cycle of hair cell and Apoptosis.This seminar is studied in the Chinese Han Population of larger samples amount to HOTAIR bases
Because of the relation between polymorphism and NIHL neurological susceptibilities, to further appreciate that the relation of HOTAIR gene pleiomorphisms and disease provides section
Theory foundation, also provides practicable theoretical foundation and scientific basis to study NIHL other tumor susceptibility genes.
The content of the invention
The technical problem of solution：It is an object of the present invention to from noise hearing impairment workman haemocyte and age-sex etc.
DNA is extracted in control group workman's haemocyte of matching, many three sites is have detected and demonstrates HOTAIR genes
Rs4759314, haplotype GGG and other haplotypes and the correlation of noise hearing loss, in this, as Noise Exposure workman
The biomarker of examination, simple to operate before hilllock, and sensitivity is high, it is adaptable to which the examination of extensive Susceptible population, is Noise Exposure work
Registration screening provides foundation before people hilllock.
Technical scheme：The haemocyte genetic marker object detecting method of Noise Exposure workman, the haemocyte base
Because (biology) mark is：HOTAIR gene rs4759314 pleomorphism sites；The rs4759314 of the HOTAIR genes is polymorphic
Property locus gene be SEQ ID NO:Shown in 1：GCTTGGAAGGGATATAAACAGGCCA[A/G]
GCGGATGCAAGTTAATAAAACCTGA。
Described gene marker is applied in the kit for preparing detection, and described application refers to by detecting testee
The rs4759314 polymorphic position point genes of HOTAIR genes, so as to judge whether it is the high-risk of noise hearing loss group
Crowd.
Applied in the kit for the preparation detection stated, pass through the HOTAIR genes of detection noise hearing impairment group
Genotype, haplotype GGG and other haplotypes in rs4759314 sites, and relatively carry out Noise Exposure work compared with control group
Examination before people hilllock.
Three sites of HOTAIR genes are have detected the invention reside in the method using SNP Genotypings and are demonstrated
HOTAIR gene rs4759314 sites, haplotype GGG or other haplotypes and the correlation of noise hearing loss.So as to
Judge its whether be Noise Exposure people at highest risk.
Beneficial effect：Control group workman's blood that the present invention is matched from noise hearing impairment workman haemocyte and age-sex etc.
DNA is extracted in cell, many three sites is have detected and demonstrates HOTAIR genes rs4759314, haplotype GGG and other lists
The correlation of times type and noise hearing loss, in this, as the biomarker of examination before Noise Exposure workman hilllock, operation letter
Single, sensitivity is high, it is adaptable to the examination of extensive Susceptible population, and foundation is provided for registration screening before Noise Exposure workman hilllock.
Embodiment
The invention will be further elaborated by the following examples.The present embodiment is premised on technical solution of the present invention
It is lower to be implemented, detailed embodiment and process is given, but the scope of the present invention is not limited to following embodiments.Under
Unreceipted condition and method etc. are carried out according to the condition proposed by routine or manufacturer in the embodiment in face.
Embodiment 1：
Selecting research object
Using Case-control Study Method, the Chinese Han nationality clarified a diagnosis through electric audiometry result and Exposed history is collected
NIHL patient totally 570；Frequency matched and and case are carried out according to sex, age, noise exposure level and exposure time limit feature
With the normal healthy controls 570 of factory.
Survey and audiometry
Investigation content includes inquiring and recording ordinary circumstance, occupational history, individual protection situation, family history, medication history, smoking
History of drinking history etc..All objects are required to depart from noise circumstance more than at least 12-48 hours before being examined, and are less than in background values noise
In 25dB (A) soundproof room, respectively to the left and right ear 500,1 of all objects progress, 000,2,000,3,000,4,000,6,
The pure tone air conduction hearing threshold test of 000Hz totally 6 frequencies, speech frequency hearing loss and height are calculated after then carrying out age and sex correction
Frequency hearing loss.
The measure of patient-specific susceptibility amount
According to《Workplace physical factor measurement noise》(GBZ/T189.8.2007), using Quest NoisePro-DL
Type multifunctional personal noise dose meter (Quest, USA) detects Noise Exposure intensity in all research object operating environments, measurement
Immediate record measured value and the Occupational Hazard Factors that may influence hearing system is identified after end.
Peripheral blood is gathered and pretreatment and extraction
Blood specimen collection and pre-treatment collection research object early morning empty stomach ulnar vein blood 5.0ml, are placed in liquaemin from arteriovenous
Ice chest, which is placed in, in heparin tube, after fully shaking up takes back laboratory.800 × g centrifuges 10min, separation serum and haemocyte at room temperature,
Haemocyte is used to extract genomic DNA.Carried out using QIAamp 96DNA QIAcube HT Kit kits according to operation manual
Peripheral blood cells extracting genome DNA, is detected with Nanodrop OneC ultramicron ultraviolet specrophotometers, is as a result shown:
Absorbance (A) 260/A280 illustrates that extracted DNA purity is higher between 1.8-2.0, available for next step experiment.
Gene SNP parting statistical analysis
In this research, statistical analysis is carried out using the softwares of SPSS 23.0.We calculate noise hearing loss group first
And Harry's Weinberg equilibrium numerical value (P of control group>0.05) to ensure that two groups of research objects derive from same crowd.Adjusting
It has suffered sex, after the age, this seminar calculates the gene frequency of genetic loci of interest using logistic regression analyses
Odds ratio (odds ratio, OR) and 95% credibility interval (95%confidence between susceptibility,noise
intervals,CIs).In blood noise concentration, we use bilateral Chi-square Test, other data we then employ sided t
Inspection is compared.
Haemocyte gene SNP classifying method
(1) DNA extraction
Peripheral blood cells genome is carried out according to operation manual using QIAamp 96DNA QIAcube HT Kit kits
DNA is extracted, and is detected with Nanodrop OneC ultramicron ultraviolet specrophotometers.
(2) SNP partings
Genotyping program is further carried out by the real-time PCR systems of ABI 7900.Real-time PCR condition is as follows：95 DEG C,
10min；95 DEG C, 15s；60 DEG C, 1 minute (most latter two steps are 40 circulations).
As a result：Demography and the clinical data description of research object,
The essential characteristic of study population is listed in table 1. in noise hearing loss group and control group, sex, smoking state
With no significant difference of drinking.Noise hearing loss group and control group there were significant differences in Dosimeter (p<
0.001).It is significantly higher than control group (p in ear disease history and family's history of disease noise hearing loss group<0.05).
The Noise Exposure workman demography of table 1 and clinical data
Gene pleiomorphism and the correlation analysis of noise induced hearing loss
By the age, sex educates the genotype of SNP in noise hearing loss and control group that adjustment is drunk with alcohol
And gene frequency.Balanced in Noise Exposure workman in the distribution of genotype without departing from Hardy-Weinberg.According to
As a result, only HOTAIR rs 4759314 have statistical significance in noise resistance group and noise tolerance group workman.With compareing
Group is compared, and G allele is frequent in noise hearing loss group, and carries out (7.2% pair of statistical analysis as risk factors
4.7%, P=0.016, OR=1.54).In addition, AG/GG carrier compares in noise hearing loss group and control group, hold
Carry on a shoulder pole 1.5 times of noise poisoning risks (P=0.031, OR=1.51).There is no other gene pleiomorphism display noise tolerance groups and make an uproar
Had differences between acoustic reactance group.
SNPs genotype frequency in the Noise Exposure workman of table 2.
The gene pleiomorphisms of rs 4759314 and the correlation analysis of Noise Exposure workman's essential characteristic
In allele AA groups and allele AG/GG groups, sex and is drunk and has significant difference at smoking state.Deng
There were significant differences in Dosimeter (p=0.011) in position Gene A A groups and allele AG/GG groups.In ear disease
History and ear disease family history position allele AA groups are all higher than allele AG/GG groups (p<0.05).Band earplug situation equipotential
Gene A A groups all hell allele AG/GG groups (p<0.05).
HOTAIR haplotypes and the relation of NIHL neurological susceptibilities
The present invention carries out haplotype analysis using Haploview softwares and SAS softwares to HOTAIR SNPs sites.With
Haplotype GAC is compared, and the risk that HOTAIR GGG haplotypes and other haplotypes suffer from NIHL is higher, points out it may be for NIHL's
Hazards (P<0.05).
Described application detects the HOTAIR gene rs4759314 sites of testee using the method for SNP Genotypings
Genotype, haplotype GGG and other haplotypes.Contain such as SEQ ID NO in the kit:The RT of HOTAIR shown in 2
Primer, rs4759314-F:ATGATCTGCTTGGAAGGGATATAAA
rs4759314-R:GCCTGGGCTTTTTCAGGTTT；
Shown HOTAIR F ends primer：
rs4759314-P-A:FAM-AGGCCAAGCGGAT-MGB
rs4759314-P-G:HEX-AGGCCAGGCGGAT-MGB。
The foregoing examples are merely illustrative of the technical concept and features of the invention, its object is to allow the person skilled in the art to be
Present disclosure can be understood and implemented according to this, it is not intended to limit the scope of the present invention.It is all smart according to the present invention
Equivalent transformation or modification that refreshing essence is done, should all be included within the scope of the present invention.
SEQ ID NO:1：GCTTGGAAGGGATATAAACAGGCCA[A/G]GCGGATGCAAGTTAATAAAACCTGA
SEQ ID NO:2：rs4759314-F: ATGATCTGCTTGGAAGGGATATAAA
rs4759314-R: GCCTGGGCTTTTTCAGGTTT
SEQ ID NO:3：rs4759314-P-A: FAM-AGGCCAAGCGGAT- MGB
rs4759314-P-G: HEX-AGGCCAGGCGGAT-MGB
Claims (5)
1. the haemocyte genetic marker object detecting method of Noise Exposure workman, it is characterised in that the haemocyte gene biological mark
Will thing is：HOTAIR gene rs4759314 pleomorphism sites；The rs4759314 polymorphic position point genes of the HOTAIR genes
For SEQ ID NO:Shown in 1：GCTTGGAAGGGATATAAACAGGCCA[A/G]GCGGATGCAAGTTAATAAAACCTGA.
2. the biomarker described in claim 1 is applied in the kit for preparing detection, described application refers to by detection
The rs4759314 polymorphic position point genes of testee's HOTAIR genes, so as to judge whether it is noise hearing loss
The people at highest risk of group.
3. applied in the kit according to claim 2 for preparing detection, it is characterised in that pass through detection noise hearing
Genotype, haplotype GGG and other haplotypes in the HOTAIR gene rs4759314 sites of damage group, and compared with control group
Relatively carry out examination before Noise Exposure workman hilllock.
4. application according to claim 2, it is characterised in that HOTAIR genes have detected using the method for SNP Genotypings
Three sites and demonstrate HOTAIR gene rs4759314 sites, haplotype GGG or other haplotypes and noise-induced hearing and damage
The correlation of wound.
5. application according to claim 2, it is characterised in that contain such as SEQ ID NO in the kit:Shown in 2
HOTAIR RT primers：rs4759314-F:ATGATCTGCTTGGAAGGGATATAAA
rs4759314-R:GCCTGGGCTTTTTCAGGTTT
Shown HOTAIR F ends primer：
rs4759314-P-A:FAM-AGGCCAAGCGGAT-MGB
rs4759314-P-G:HEX-AGGCCAGGCGGAT-MGB。
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110093414A (en) * | 2019-04-09 | 2019-08-06 | 东南大学 | Registration screening biomarker and its application before Noise Exposure worker hilllock |
| CN111584065A (en) * | 2020-04-07 | 2020-08-25 | 上海交通大学医学院附属第九人民医院 | Noise hearing loss prediction and susceptible population screening method, device, terminal and medium |
| CN111593108A (en) * | 2020-03-12 | 2020-08-28 | 中国人民解放军总医院第五医学中心 | Method and kit for detecting polymorphism of 7q36.3 region associated with occurrence of noise-induced hearing loss, and application thereof |
| CN113736872A (en) * | 2020-05-29 | 2021-12-03 | 上海交通大学医学院附属第九人民医院 | Biomarker for predicting susceptibility risk of noise-induced hearing loss and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642921A (en) * | 2013-12-09 | 2014-03-19 | 中国人民解放军第三军医大学第三附属医院 | Selection, detection and application of catalase gene tagging single nucleotide polymorphic sites |
| CN106244696A (en) * | 2016-08-18 | 2016-12-21 | 中南大学 | A kind of biomarker relevant to lung cancer susceptibility and application |
-
2017
- 2017-04-01 CN CN201710214588.4A patent/CN107119113A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642921A (en) * | 2013-12-09 | 2014-03-19 | 中国人民解放军第三军医大学第三附属医院 | Selection, detection and application of catalase gene tagging single nucleotide polymorphic sites |
| CN106244696A (en) * | 2016-08-18 | 2016-12-21 | 中南大学 | A kind of biomarker relevant to lung cancer susceptibility and application |
Non-Patent Citations (3)
| Title |
|---|
| BOSHEN WANG等: "Association of TagSNP in lncRNA HOTAIR with susceptibility to noise-induced hearing loss in a Chinese population", 《HEARING RESEARCH》 * |
| 杨伟炎: "《中华医学会第九次全国耳鼻咽喉 头颈外科学术会议论文汇编》", 31 October 2005 * |
| 闫芮: "长链非编码RNA HOTAIR、H19和SRA SNPs与乳腺癌遗传易感性关联研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110093414A (en) * | 2019-04-09 | 2019-08-06 | 东南大学 | Registration screening biomarker and its application before Noise Exposure worker hilllock |
| CN110093414B (en) * | 2019-04-09 | 2022-06-24 | 东南大学 | Noise exposure worker pre-post enrollment screening biomarker and application thereof |
| CN111593108A (en) * | 2020-03-12 | 2020-08-28 | 中国人民解放军总医院第五医学中心 | Method and kit for detecting polymorphism of 7q36.3 region associated with occurrence of noise-induced hearing loss, and application thereof |
| CN111593108B (en) * | 2020-03-12 | 2022-10-14 | 中国人民解放军总医院第五医学中心 | Method and kit for detecting polymorphism of 7q36.3 region associated with occurrence of noise-induced hearing loss, and use thereof |
| CN111584065A (en) * | 2020-04-07 | 2020-08-25 | 上海交通大学医学院附属第九人民医院 | Noise hearing loss prediction and susceptible population screening method, device, terminal and medium |
| CN111584065B (en) * | 2020-04-07 | 2023-09-19 | 上海交通大学医学院附属第九人民医院 | Noise hearing loss prediction and susceptible crowd screening method, device, terminal and medium |
| CN113736872A (en) * | 2020-05-29 | 2021-12-03 | 上海交通大学医学院附属第九人民医院 | Biomarker for predicting susceptibility risk of noise-induced hearing loss and application |
| CN113736872B (en) * | 2020-05-29 | 2023-07-14 | 上海交通大学医学院附属第九人民医院 | Biomarker for predicting susceptibility risk of noise hearing loss and application |
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Application publication date: 20170901 |