CN107083401A - A kind of resource utilization method of agricultural organic waste - Google Patents
A kind of resource utilization method of agricultural organic waste Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H6/00—Macromolecular compounds derived from lignin, e.g. tannins, humic acids
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention discloses a kind of resource utilization method of agricultural organic waste, it is that ferriferous oxide is first added in agricultural organic waste, then carries out anaerobic dry fermentation generation biogas, and residual residue is separated into biogas slurry and biogas residue after washing；Biogas slurry obtains inoculation liquid as nutrient solution culture saccharomyces cerevisiae；Biogas residue is converted into dissolubility sugar by cellulase, and is separated into solid residue and sugar juice；Solid residue in inoculation liquid and sugar juice mixed fermentation, products therefrom is used for the raw material as anaerobic dry fermentation, and liquid obtains ethanol through distillation；Solid residue is used for output lignin.Organic waste efficiently can be switched to methane, three kinds of products of ethanol and lignin by the present invention, realize resource it is efficient using and added value lifting, and technique greenization, clean that level is higher, full technique is without any waste discharge.
Description
Technical field
The present invention relates to environmental protection and recycling field, and in particular to a kind of recycling of agricultural organic waste
Method.
Background technology
Developed rapidly with economical and society, China produces substantial amounts of agricultural wastes every year.In addition with people's people's livelihood
The flat raising of running water, aquaculture is developed rapidly, thus produces substantial amounts of fowl and animal excrement.These discarded objects can under anaerobic environment
Produce biogas, biogas slurry and biogas residue.Biogas can be used for generating electricity, warms oneself after desulfurization removing nitric processing in traditional handicraft.Natural pond
Liquid and biogas residue can be used for agricultural production as green fertilizer.
Anaerobic dry fermentation is also known as solid anaerobic fermentation, is one of main technique therein, and the total solid in reaction system contains
Amount can reach 20%~40%.Had the advantage that relative to conventional anaerobic fermentation：Reactor volume is smaller, and course of reaction needs
Heating and physical mechanical energy expenditure it is less, the energy loss of process is less.But current common problem is organic
The anaerobic methane production speed of discarded object is relatively low, methane purity is low, long processing period, efficiency are low.It mainly restricts methane generation
One of factor is that in course of reaction that hydrolysed ferment bacterial reproduction speed is slower, and the abundant fatty acid that high concentration substrate is produced is not
By methanogen using absorbing local acid accumulation can be caused to cause pH relatively low, hydrolysed ferment bacterium and methanogen are produced
Raw stronger inhibitory action.Improved even in using preconditioning techniques such as physics, chemistry, biologies before substrate biodegradable
Put, the methane generation speed of anaerobic dry fermentation process is still relatively low, far below theoretical methane production.
On the other hand, current biogas slurry, biogas residue utilization ways it is relatively limited, lack the conversion of high-valued product.Such as, utilize
Biogas residue and biogas liquid production actinomyces fertilizer (2012104679296), slow-releasing granules organic fertilizer (2012105757610), cultivation are sowed in spring
Mushroom (CN104541981B) and pleurotus cornucopiae (CN103348868B), organic fertilizer (CN104086238B) is prepared with charcoal doping,
Biogas residue aerobic fermentation prepares biological organic fertilizer (CN103848698B), for microdisk electrode CN103194395A) etc. purposes.- and
The research for preparing chemical raw material or energy products using biogas residue is seldom, only a small amount of to prepare ethanol (agricultural machinery using biogas residue
Journal, 2015,46 (5)：156-163), biodiesel (Biotechnol Biofuels, 2016, (9):253), biological pesticide
(EI, 2013,29 (8)：Research report 212-217), has a disadvantage in that the yield of product is relatively low, and prepare
Technique still can produce waste residue.But if regulating and controlling to anaerobic dry fermentation process, it is possible to which that improves biogas residue can resource
Change utilization ways and efficiency.Therefore how to implement to regulate and control to realize the generation of methane and biogas residue, exist for anaerobic dry fermentation technology
The application and popularization for handling organic waste are major issues urgently to be resolved hurrily.
The content of the invention
To avoid the weak point present in above-mentioned prior art, the invention provides a kind of agricultural organic waste resource
Change the method for comprehensive utilization, it is intended to solve the utilization ways of low methane production in existing anaerobic dry fermentation technique, biogas slurry and biogas residue
The problem of limitation.
To solve technical problem, the present invention is adopted the following technical scheme that：
The resource utilization method of agricultural organic waste of the present invention, its feature is：First in agricultural organic waste
Ferriferous oxide is added, then carries out anaerobic dry fermentation and produces biogas, residual residue is separated into biogas slurry and biogas residue after washing；The natural pond
Liquid obtains inoculation liquid as nutrient solution culture saccharomyces cerevisiae；The biogas residue is converted into dissolubility sugar by cellulase, and separates
For solid residue and sugar juice；Solid residue in the inoculation liquid and the sugar juice mixed fermentation, products therefrom is used for
As the raw material of anaerobic dry fermentation, liquid obtains ethanol through distillation；The solid residue is used for output lignin.Specifically include as
Lower step：
(1) using agricultural organic waste as raw material, ferriferous oxide is added wherein, and add suitable quantity of water regulation and control dry to contain
Amount, then carries out anaerobic dry fermentation, and fermentation temperature is 50-70 DEG C, collects the biogas produced in fermentation process；Biogas can be direct
Utilize, or for combustion power generation etc. after processing；The addition of the ferriferous oxide is the agricultural organic waste in terms of Fe
The 0.2-5% of middle volatile solid quality；
(2) remaining residue after washing step (1) fermentation is soaked, then using the method separation of centrifugation or filtering,
Obtain biogas slurry and biogas residue；
(3) after biogas slurry obtained by step (2) is handled through high-temperature sterilization, as nutrient solution culture saccharomyces cerevisiae, it is inoculated with
Liquid；
(4) biogas residue obtained by step (2) is added in sig water and carries out immersion treatment, then separated and sterilize；By alkali process
Biogas residue is added to 0.05molL afterwards-1, pH 4.8 citric acid solution in, add cellulase, seal, be subsequently placed in
In thermostatic control oscillator vibration, 50 DEG C of culture 24-72h are then centrifuged for separation, obtain solid residue and sugar juice；
(5) sugar juice in the inoculation liquid in step (3) and step (4) is mixed, makes amount of yeast in gained mixed liquor
It is 10-25gL for 1.5-2.0g/L (being based on dry matter content), initial sugared content-1, then normal temperature fermentation 24-48h, solid-liquid point
From gained solid residue is used for the raw material as step (1) anaerobic dry fermentation, and gained liquid obtains ethanol, distillation through distillation
Remaining aqueous solution is used to regulate and control dry matter content in step (1) and/or for washing by soaking in step (2) afterwards；
(6) solid residue obtained by washing step (4) is soaked, then using the method separation of solid and liquid of centrifugation or filtering,
Solid is the product of high lignin content, and remaining aqueous solution is used to regulate and control dry matter content in step (1) and/or for step
(2) washing by soaking in.
It is preferred that, described agricultural organic waste includes plant's fowl and animal excrement, agricultural production discarded object, production of forestry
At least one of discarded object, gardens industrial waste and domestic waste.
It is preferred that, described ferriferous oxide is selected from least one of limonite, goethite, bloodstone and magnetic iron ore.
It is preferred that, the carbon-nitrogen mass ratio of described agricultural organic waste is 20~25:1.
It is preferred that, the dry matter content described in step (1) is 20-50%.
It is preferred that, the quality that added water in step (2) is 2-4 times of remaining residue dry biomass after step (1) is fermented.
It is preferred that, step (4) described sig water is mass concentration 1-3% NaOH solution.
It is preferred that, the addition of step (4) cellulase is calculated according to the dry matter content of biogas residue after alkali process, is added
Measure as 5-30FPU/g.
Compared with the prior art, beneficial effects of the present invention are embodied in：
1st, organic waste efficiently can be switched to methane, three kinds of products of ethanol and lignin by the present invention, be realized useless
The efficient of goods and materials source is lifted using with added value.
2nd, technique greenization of the invention, to clean level higher, by front and rear technique adjustment, by each step produce it is useless
Thing becomes the raw material of subsequent technique, the discarded object that subsequent technique is produced can as front-end process raw material or assistant product, entirely
Technique is without any waste discharge.
3rd, the present invention regulates and controls the anaerobic high temperature dry fermentation process of agricultural organic waste using ferriferous oxide, to improve biogas residue
Component and structure, improve the process yields that biogas residue prepares ethanol, improve the purity of lignin product.
Brief description of the drawings
Fig. 1 is the process chart of agricultural organic waste resource utilization method of the present invention；
Fig. 2 is that the methane yield of each group reactor in embodiment 1 compares figure；
Fig. 3 is the lignocellulosic composition analysis result pair of residual residue after each group reactor fermentation ends in embodiment 1
Than；
Fig. 4 is the reducing sugar yield contrast of each group reactor biogas residue enzymolysis process in embodiment 1；
The lignocellulosic composition analysis result pair of Fig. 5 solid residues obtained by after each group reactor enzymolysis in embodiment 1
Than；
Fig. 6 contrasts for concentration of alcohol in solution after each group reactor enzymolysis liquid fermentation in embodiment 1.
Embodiment
The method of the present invention is illustrated by embodiment below in conjunction with the accompanying drawings, but the invention is not limited in
This.
In following embodiments, cow dung is derived from Hefei in Anhui countryside dairy cow farm, and wherein mass fraction of solids is
10.9%.
In following embodiments, maize straw is taken from Hefei ,Anhui city Feidong County suburb farmland, and stalk is through Mechanical Crushing, mistake
It is standby after 1mm sieves, labeled as stalk 1.20g stalks 1 are added in the dilution heat of sulfuric acid that 200mL mass concentrations are 0.75%,
80min is cleared up at 150 DEG C；After the completion of clearing up, room temperature, regulation pH to neutrality are cooled to；Gained digestion solution is centrifuged, gained
Solid precipitation is again with standby after distillation water washing 2 times, labeled as stalk 2, as a comparison.
In following embodiments, ferriferous oxide used is crushed by goethite, crosses the acquisition of 100 mesh sieves.
In following embodiments, saccharomyces cerevisiae used is Saccharomyces cerevisiae D5A yeast, and purchase is certainly beautiful
State's type culture collection warehousing (American Type Culture Collection).
Embodiment 1
The present embodiment carries out recycling to agricultural organic waste as follows：
1st, anaerobic dry fermentation is tested
Using 250mL serum bottles as reactor, 12 reactors are set altogether, are divided into four groups, every group sets 3 Duplicate Samples,
Agricultural organic waste is respectively used in each group：5g stalk 1+1.25g cow dungs (being based on amount of dry matter), 5g stalk 1+1.25g oxen
Excrement+ferriferous oxide (addition in terms of Fe, be in stalk 1 and cow dung volatile solid gross mass 0.2%), 5g stalks 2+
1.25g cow dungs, 5g stalks 1+1.25g cow dungs+ferriferous oxide (are that volatile solid is total in stalk 1 and cow dung addition in terms of Fe
Quality 5%).Plus dry matter content is 20% in suitable quantity of water regulation and control each group reactor solution.
Anaerobic dry fermentation is carried out to each group reactor, fermentation temperature is 55 DEG C, measures the gas body produced in fermentation process
Product and methane content, methane output result are as shown in Figure 2.
Wood fibre cellulose content is determined after remaining residue is sampled and washed with 100mL after fermentation, as a result such as Fig. 3 institutes
Show.
2nd, the washing of residue and separating step：
Remaining residue is soaked after anaerobic dry fermentation, and the quality of water is according to 3 times of additions of amount of dry matter in residue, leaching
Bubble 10 hours, then (can be using filtering for the larger residue of stalk particle diameter using the method separation of solid and liquid of centrifugation or filtering
Method separation；, can be using the side centrifuged when easily being blocked using filter method for the less residue of stalk particle diameter
Method), obtain biogas residue and biogas slurry.
3rd, the alkali process and enzymolysis process of biogas residue：
Biogas residue is placed in the NaOH solution that mass concentration is 2% and soaked, the primary quantity of biogas residue is calculated as 50g/L according to TS,
After immersion 24 hours, biogas residue after alkali process is obtained using the method for centrifugation, sterilized.
It is 1.0g (bases that biogas residue after alkali process is placed in into biogas residue addition in progress enzymolysis experiment in 250mL saline bottles, every group
In dry matter content), add 0.05molL-1, pH 4.8 citric acid solution to dry matter content be 50g/L, sterilizing
After add cellulase, addition is 10FPU/g (being based on dry matter content).Bottleneck is clogged with butyl rubber plug, aluminium lid is sealed,
It is placed in thermostatic control oscillator vibration, in cultivating 72h at 50 DEG C.
All experimental groups set three groups of Duplicate Samples.Produced in 0,4,8,12,24,48,72h sampling analysis measurings reduced sugar
Amount, as a result as shown in Figure 4.
Solution centrifugal is separated after enzymolysis, obtains solid residue and sugar juice.
After solid residue is using the cleaning of 100mL water, wood fibre cellulose content is determined.As a result it is as shown in Figure 5.
4th, biogas slurry is used for culture yeasts：
After biogas slurry in step 2 is handled through high-temperature sterilization, it is used to cultivate Saccharomyces as nutrient solution
Cerevisiae D5A yeast, the addition of initial yeast bacterium is 10g/L (being based on yeast dry matter content), at room temperature (25-30
DEG C) culture obtains yeast bacteria concentration in inoculation liquid, inoculation liquid after 24-48 hour 15-20g/L can be reached (based on yeast dry
Matter content).
5th, yeast fermenting experiment：Sugar juice in inoculation liquid and step 3 is mixed according to proper proportion, makes gained mixed liquor
The concentration of middle yeast juice is that 1.5-2.0g/L (be based on yeast dry matter content), initial sugared content are 14-25gL-1, in normal temperature
Lower fermentation 24 hours, carries out separation of solid and liquid.Gained solid residue can be used as ethanol in the raw material of anaerobic dry fermentation, resulting solution
Content is as shown in fig. 6, resulting solution obtains ethanol through separated, and remaining aqueous solution can be used for regulation anaerobism production first after distillation
Solid content or washing biogas residue etc. in alkane reactor.
As shown in Figure 2, stalk and cow dung mixing dry fermentation, iron oxidation is added using acid treatment stalk and in mixed system
Thing can all be obviously promoted the generation of methane.The total gas production of stalk 1 and cow dung mixed fermentation is about 500mL, after acid treatment
The total gas production of stalk 2 and cow dung mixed fermentation is about 1050mL, adds the gas production more than one times.Add in mixed system
The generation of methane can be promoted by entering ferriferous oxide, and the different generations to methane of ferriferous oxide addition have a great impact, and mix
The total gas production that methane after 0.2% ferriferous oxide is added in zoarium system is about 800mL, and the ferriferous oxide of addition 5% is total
Gas production is about 1150mL, and the ferriferous oxide of addition 5% adds 350mL gas production than adding 0.2% ferriferous oxide, because
This understands that production of the increase of ferriferous oxide amount to methane more has facilitation in certain limit；Added in mixed system
0.2% ferriferous oxide adds about 300mL gas production than original stalk fermentation, and the ferriferous oxide of addition 5% is than original
Stalk fermentation adds about 650mL gas production, and incrementss are 1.3 times of original stalk fermentation total gas production.
From the figure 3, it may be seen that stalk adds ferriferous oxide through peracid treatment and in mixed system to biogas residue component after dry fermentation
There is large effect.After original stalk 1 is fermented, the content difference of cellulose, hemicellulose and lignin in its biogas residue component
It is not very big, cellulose and content of lignin quite, about respectively account for the 22% of total component, and hemicellulose level is relatively fewer,
About 15%；Content of cellulose is of a relatively high in the ferriferous oxide of addition 0.2%, its biogas residue in mixed system, and about 23%, and
Hemicellulose and content of lignin are respectively then 5% and 12%；5% ferriferous oxide is added in mixed system and through peracid treatment
Stalk, after the completion of fermentation, the content of three has very big difference in its biogas residue：Add after 5% ferriferous oxide, it is wooden in its biogas residue
The content of quality has reached 37%；The content of lignin has then reached 41% in stalk after acid treatment, its biogas residue；And the two body
The content of cellulose and hemicellulose in system is then seldom, all less than 10%, the hemicellulose added in 5% ferriferous oxide system
Even more it is completely broken down.
As shown in Figure 4, the enzymolysis process of biogas residue, its reducing sugar yield increases with the growth of time, thus passes through enzymolysis
It is feasible that process, which obtains reduced sugar,.Ferriferous oxide is added in former fermentation system, producing reduced sugar to follow-up biogas residue enzymolysis has
Its reducing sugar yield about 15mgmL after biogas residue after facilitation, former stalk fermentation is digested through 72h-1, and add 0.2% iron
The reducing sugar yield of oxide system is about 22mgmL-1, the reducing sugar yield for adding 5% ferriferous oxide system is about 26mg
mL-1, reducing sugar yield adds about 0.46 times and 0.73 times respectively, and then it can also be seen that reducing sugar yield is aoxidized with iron
The increase of thing content and increase；Stalk is through peracid treatment, and the biogas residue after the completion of its fermentation is to enzymolysis reducing sugar yield without too big
Influence.
As shown in Figure 5, after biogas residue reclaims reduced sugar through enzymolysis, the lignin component content in its remaining solid residue is accounted for always
The significant portion ratio of component, all more than 80%, therefore it is feasible to reclaim lignin in residue.Acid treatment stalk and in original
Ferriferous oxide is added in fermentation system, the recovery to follow-up lignin has a certain impact.Stalk 2 after acid treatment and in system
Middle addition ferriferous oxide, lignin component content can all have increase, while cellulose and hemi-cellulose components content have necessarily
Reduction；In the fermentation system for adding 5% ferriferous oxide, fiber has not been contained substantially in the solid residue after biogas residue enzymolysis
Element and hemicellulose, lignin component content have reached about 94%.
It will be appreciated from fig. 6 that biogas slurry digests the sugar juice obtained after Yeast Cultivation with biogas residue carries out mixed fermentation, fermentation is completed
Ethanol content in separation of solid and liquid, obtained liquid phase is carried out to fermentation system afterwards as follows：The ethanol content that original stalk is finally reclaimed
About 7.5mgmL-1, the system for adding 5% ferriferous oxide is about 12.5mgmL-1, add the system of 0.2% ferriferous oxide
About 11.0mgmL-1, ethanol content adds about 0.67 times and 0.47 times respectively, and this also illustrates that ethanol content is aoxidized with iron
The increase of object amount and increase.Liquid phase can be recovered to ethanol after distillation, illustrate that biogas slurry is mixed after Yeast Cultivation with sugar juice
It is feasible that ethanol is reclaimed in fermentation.
, can be real by the coupling of anaerobic dry fermentation, enzymolysis and alcohol fermentation technique from above-mentioned Measurement results
Existing organic waste prepares methane, three kinds of products of lignin and ethanol.Ferriferous oxide is added during anaerobic dry fermentation favourable
The content of lignin that residue is obtained in the yield for improving methane, enzymolysis process is obviously improved, corresponding sugar juice system fermentation
The ethanol content obtained afterwards is obviously improved.
These are only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Any modifications, equivalent substitutions and improvements made within principle etc., should be included in the scope of the protection.
Claims (9)
1. a kind of resource utilization method of agricultural organic waste, it is characterised in that：First added in agricultural organic waste
Ferriferous oxide, then anaerobic dry fermentation generation biogas is carried out, residual residue is separated into biogas slurry and biogas residue after washing；The biogas slurry is made
For nutrient solution culture saccharomyces cerevisiae, inoculation liquid is obtained；The biogas residue is converted into dissolubility sugar by cellulase, and is separated into solid
Body residue and sugar juice；Solid residue in the inoculation liquid and the sugar juice mixed fermentation, products therefrom is used for conduct
The raw material of anaerobic dry fermentation, liquid obtains ethanol through distillation；The solid residue is used for output lignin.
2. the resource utilization method of agricultural organic waste according to claim 1, it is characterised in that including following step
Suddenly：
(1) using agricultural organic waste as raw material, ferriferous oxide is added wherein, and adds suitable quantity of water regulation and control dry matter content, so
After carry out anaerobic dry fermentation, fermentation temperature is 50-70 DEG C, collects the biogas produced in fermentation process；The ferriferous oxide plus
Enter amount in terms of Fe, be the 0.2-5% of volatile solid quality in the agricultural organic waste；
(2) remaining residue after washing step (1) fermentation is soaked, then using the method separation of centrifugation or filtering, is obtained
Biogas slurry and biogas residue；
(3) after biogas slurry obtained by step (2) is handled through high-temperature sterilization, as nutrient solution culture saccharomyces cerevisiae, inoculation liquid is obtained；
(4) biogas residue obtained by step (2) is added in sig water and carries out immersion treatment, then separated and sterilize；By natural pond after alkali process
Slag is added to 0.05molL-1, pH 4.8 citric acid solution in, add cellulase, seal, be subsequently placed in constant temperature
In water bath chader, 50 DEG C of culture 24-72h are then centrifuged for separation, obtain solid residue and sugar juice；
(5) sugar juice in the inoculation liquid in step (3) and step (4) is mixed, makes the dry of yeast in gained mixed liquor
Content is that 1.5-2.0g/L, initial sugared content are 10-25gL-1, then normal temperature fermentation 24-48h, separation of solid and liquid, gained solid-state
Residue is used for the raw material as step (1) anaerobic dry fermentation, and gained liquid obtains ethanol, remaining aqueous solution after distillation through distillation
For regulation and control dry matter content in step (1) and/or for washing by soaking in step (2)；
(6) solid residue obtained by washing step (4) is soaked, then using the method separation of solid and liquid of centrifugation or filtering, solid
For the product of high lignin content, remaining aqueous solution is used to regulate and control dry matter content in step (1) and/or in step (2)
Washing by soaking.
3. the resource utilization method of agricultural organic waste according to claim 1 or 2, it is characterised in that：Described
Agricultural organic waste includes plant's fowl and animal excrement, agricultural production discarded object, production of forestry discarded object, gardens industrial waste
At least one of with domestic waste.
4. the resource utilization method of agricultural organic waste according to claim 1 or 2, it is characterised in that：Described
Ferriferous oxide is selected from least one of limonite, goethite, bloodstone and magnetic iron ore.
5. the resource utilization method of agricultural organic waste according to claim 1 or 2, it is characterised in that：Described
The carbon-nitrogen mass ratio of agricultural organic waste is 20~25:1.
6. the resource utilization method of agricultural organic waste according to claim 2, it is characterised in that：In step (1)
Described dry matter content is 20-50%.
7. the resource utilization method of agricultural organic waste according to claim 2, it is characterised in that：In step (2)
The quality that adds water is 2-4 times of remaining residue dry biomass after step (1) is fermented.
8. the resource utilization method of agricultural organic waste according to claim 2, it is characterised in that：Step (4) institute
State the NaOH solution that sig water is mass concentration 1-3%.
9. the resource utilization method of agricultural organic waste according to claim 2, it is characterised in that：In step (4)
The addition of cellulase is calculated according to the dry matter content of biogas residue after alkali process, and addition is 5-30FPU/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710484279.9A CN107083401A (en) | 2017-06-23 | 2017-06-23 | A kind of resource utilization method of agricultural organic waste |
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| CN107644742A (en) * | 2017-10-31 | 2018-01-30 | 合肥工业大学 | A kind of method that biogas residue activated carbon prepares ultracapacitor after dry fermentation based on biomass anaerobic |
| CN107824315A (en) * | 2017-10-24 | 2018-03-23 | 中矿国科（北京）科技有限公司 | It is a kind of to comprehensively utilize the method for gluing wet discarded object |
| CN108014634A (en) * | 2017-11-15 | 2018-05-11 | 广东省生态环境技术研究所 | A kind of method for accelerating methane emission reduction |
| CN108486207A (en) * | 2018-04-17 | 2018-09-04 | 淮北市菲美得环保科技有限公司 | A kind of agricultural wastes and waste sludge from paper mill federated resourceization utilize method |
| CN108517336A (en) * | 2018-03-28 | 2018-09-11 | 嘉兴学院 | A method of utilizing anaerobic fermentation processing urban afforestation lawn waste methane phase |
| CN109486864A (en) * | 2018-11-14 | 2019-03-19 | 江南大学 | A kind of solid-state alkali treatment method improving organic waste anaerobic fermentation efficiency |
| CN109836183A (en) * | 2019-03-29 | 2019-06-04 | 安徽安特治废弃物资源化研究中心有限公司 | A kind of method of house refuse aerobic fermentation-sorting recycling |
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| CN112961884A (en) * | 2021-04-07 | 2021-06-15 | 中国农业科学院农业环境与可持续发展研究所 | Method for synthesizing medium-chain fatty acid by using planting wastes |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107824315A (en) * | 2017-10-24 | 2018-03-23 | 中矿国科（北京）科技有限公司 | It is a kind of to comprehensively utilize the method for gluing wet discarded object |
| CN107644742A (en) * | 2017-10-31 | 2018-01-30 | 合肥工业大学 | A kind of method that biogas residue activated carbon prepares ultracapacitor after dry fermentation based on biomass anaerobic |
| CN108014634A (en) * | 2017-11-15 | 2018-05-11 | 广东省生态环境技术研究所 | A kind of method for accelerating methane emission reduction |
| CN108517336A (en) * | 2018-03-28 | 2018-09-11 | 嘉兴学院 | A method of utilizing anaerobic fermentation processing urban afforestation lawn waste methane phase |
| CN108486207A (en) * | 2018-04-17 | 2018-09-04 | 淮北市菲美得环保科技有限公司 | A kind of agricultural wastes and waste sludge from paper mill federated resourceization utilize method |
| CN109486864A (en) * | 2018-11-14 | 2019-03-19 | 江南大学 | A kind of solid-state alkali treatment method improving organic waste anaerobic fermentation efficiency |
| CN109836183A (en) * | 2019-03-29 | 2019-06-04 | 安徽安特治废弃物资源化研究中心有限公司 | A kind of method of house refuse aerobic fermentation-sorting recycling |
| CN111560256A (en) * | 2020-06-02 | 2020-08-21 | 潍坊工商职业学院 | Organic solid waste integrated ecological resource utilization technical method |
| CN112961884A (en) * | 2021-04-07 | 2021-06-15 | 中国农业科学院农业环境与可持续发展研究所 | Method for synthesizing medium-chain fatty acid by using planting wastes |
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