CN107075587A - 多链核酸模块自组装成有限核酸纳米结构的方法 - Google Patents
多链核酸模块自组装成有限核酸纳米结构的方法 Download PDFInfo
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- CN107075587A CN107075587A CN201580057279.XA CN201580057279A CN107075587A CN 107075587 A CN107075587 A CN 107075587A CN 201580057279 A CN201580057279 A CN 201580057279A CN 107075587 A CN107075587 A CN 107075587A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 219
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 216
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 216
- 238000000034 method Methods 0.000 title claims abstract description 81
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims abstract description 4578
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract description 4330
- 238000001338 self-assembly Methods 0.000 claims abstract description 17
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- 102000012410 DNA Ligases Human genes 0.000 claims description 10
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- 238000006366 phosphorylation reaction Methods 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical group OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 241000714933 Chryseobacterium nakagawai Species 0.000 claims description 5
- 108091000080 Phosphotransferase Proteins 0.000 claims description 4
- 230000001788 irregular Effects 0.000 claims description 4
- 102000020233 phosphotransferase Human genes 0.000 claims description 4
- 239000002157 polynucleotide Substances 0.000 claims description 4
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 claims description 2
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 claims description 2
- 239000013589 supplement Substances 0.000 claims description 2
- 125000004122 cyclic group Chemical group 0.000 claims 8
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 6
- 241000700605 Viruses Species 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
提供了多链核酸模块自组装成有限核酸纳米结构的方法，包括如下步骤：n组m条寡核苷酸单链退火形成n个核酸结构模块，所述n个核酸结构模块自组装得到有限核酸纳米结构；每组寡核苷酸单链的序列均不相同。
Description
PCT国内申请，说明书已公开。
Claims (35)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2015/090678 WO2017049573A1 (zh) | 2015-09-25 | 2015-09-25 | 多链核酸模块自组装成有限核酸纳米结构的方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107075587A true CN107075587A (zh) | 2017-08-18 |
| CN107075587B CN107075587B (zh) | 2022-07-05 |
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ID=58385761
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580057279.XA Expired - Fee Related CN107075587B (zh) | 2015-09-25 | 2015-09-25 | 多链核酸模块自组装成有限核酸纳米结构的方法 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN107075587B (zh) |
| WO (1) | WO2017049573A1 (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018218646A1 (zh) * | 2017-06-02 | 2018-12-06 | 清华大学 | Dna折纸单元分步组装法 |
| CN113671167B (zh) * | 2020-05-14 | 2024-08-02 | 北京元芯碳基集成电路研究院 | 生物电子器件及其制备方法和可控转化方法 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013006411A1 (en) * | 2011-07-01 | 2013-01-10 | President And Fellows Of Harvard College | Method for forming nanoparticles having predetermined shapes |
| WO2015006626A1 (en) * | 2013-07-10 | 2015-01-15 | President And Fellows Of Harvard College | Compositions and methods relating to nucleic acid-protein complexes |
| CN104546726A (zh) * | 2015-01-06 | 2015-04-29 | 中国人民解放军第三军医大学第二附属医院 | 一种自组装核酸纳米管制剂及制备方法和应用 |
| CN104781416A (zh) * | 2012-07-24 | 2015-07-15 | 哈佛学院院长及董事 | 核酸纳米结构的自装配 |
| CN104840966A (zh) * | 2015-05-05 | 2015-08-19 | 国家纳米科学中心 | 一种核酸纳米结构抗癌复合药物及其制备方法和应用 |
-
2015
- 2015-09-25 WO PCT/CN2015/090678 patent/WO2017049573A1/zh active Application Filing
- 2015-09-25 CN CN201580057279.XA patent/CN107075587B/zh not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013006411A1 (en) * | 2011-07-01 | 2013-01-10 | President And Fellows Of Harvard College | Method for forming nanoparticles having predetermined shapes |
| CN104781416A (zh) * | 2012-07-24 | 2015-07-15 | 哈佛学院院长及董事 | 核酸纳米结构的自装配 |
| WO2015006626A1 (en) * | 2013-07-10 | 2015-01-15 | President And Fellows Of Harvard College | Compositions and methods relating to nucleic acid-protein complexes |
| CN104546726A (zh) * | 2015-01-06 | 2015-04-29 | 中国人民解放军第三军医大学第二附属医院 | 一种自组装核酸纳米管制剂及制备方法和应用 |
| CN104840966A (zh) * | 2015-05-05 | 2015-08-19 | 国家纳米科学中心 | 一种核酸纳米结构抗癌复合药物及其制备方法和应用 |
Non-Patent Citations (2)
| Title |
|---|
| ERIK WINFREE等: "Design and self-assembly of", 《NATURE》 * |
| 余多慰等编著: "《分子生物学》", 31 July 2007, 南京师范大学出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017049573A1 (zh) | 2017-03-30 |
| CN107075587B (zh) | 2022-07-05 |
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Granted publication date: 20220705 |