CN107064499A - 禽流感病毒np蛋白抗原表位多肽及其胶体金试纸盒 - Google Patents
禽流感病毒np蛋白抗原表位多肽及其胶体金试纸盒 Download PDFInfo
- Publication number
- CN107064499A CN107064499A CN201710152279.9A CN201710152279A CN107064499A CN 107064499 A CN107064499 A CN 107064499A CN 201710152279 A CN201710152279 A CN 201710152279A CN 107064499 A CN107064499 A CN 107064499A
- Authority
- CN
- China
- Prior art keywords
- influenza virus
- avian influenza
- antigen epitope
- epitope polypeptide
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000712461 unidentified influenza virus Species 0.000 title claims abstract description 65
- 238000012360 testing method Methods 0.000 title claims abstract description 45
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 239000000427 antigen Substances 0.000 title claims abstract description 31
- 102000036639 antigens Human genes 0.000 title claims abstract description 31
- 108091007433 antigens Proteins 0.000 title claims abstract description 31
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 28
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 23
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 23
- 239000010931 gold Substances 0.000 title claims abstract description 16
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 16
- 239000000084 colloidal system Substances 0.000 title claims abstract description 7
- 108010061100 Nucleoproteins Proteins 0.000 title abstract description 35
- 102000011931 Nucleoproteins Human genes 0.000 title abstract description 35
- 241001473386 H9N2 subtype Species 0.000 claims abstract description 20
- 238000001514 detection method Methods 0.000 claims abstract description 18
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 3
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000002372 labelling Methods 0.000 claims description 8
- 230000008878 coupling Effects 0.000 claims description 7
- 238000010168 coupling process Methods 0.000 claims description 7
- 238000005859 coupling reaction Methods 0.000 claims description 7
- 239000002773 nucleotide Substances 0.000 claims description 7
- 125000003729 nucleotide group Chemical group 0.000 claims description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 6
- 230000000274 adsorptive effect Effects 0.000 claims description 6
- 238000003317 immunochromatography Methods 0.000 claims description 6
- 206010022000 influenza Diseases 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 238000003259 recombinant expression Methods 0.000 claims 3
- 239000002253 acid Substances 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- 125000003835 nucleoside group Chemical group 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
- 102000040430 polynucleotide Human genes 0.000 claims 1
- 108091033319 polynucleotide Proteins 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 16
- 206010064097 avian influenza Diseases 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 abstract 1
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 1
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 28
- 150000001413 amino acids Chemical class 0.000 description 15
- 238000010790 dilution Methods 0.000 description 14
- 239000012895 dilution Substances 0.000 description 14
- 239000000020 Nitrocellulose Substances 0.000 description 13
- 229920001220 nitrocellulos Polymers 0.000 description 13
- 241000700605 Viruses Species 0.000 description 12
- 229940024606 amino acid Drugs 0.000 description 12
- 238000002965 ELISA Methods 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 241000287828 Gallus gallus Species 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- 241001515965 unidentified phage Species 0.000 description 8
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 206010003445 Ascites Diseases 0.000 description 6
- 210000003555 cloaca Anatomy 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 229920000742 Cotton Polymers 0.000 description 5
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 5
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000004408 hybridoma Anatomy 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 230000028327 secretion Effects 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 230000003612 virological effect Effects 0.000 description 5
- 208000002979 Influenza in Birds Diseases 0.000 description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 108010033040 Histones Proteins 0.000 description 3
- 239000002671 adjuvant Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 2
- 229910004042 HAuCl4 Inorganic materials 0.000 description 2
- 101710154606 Hemagglutinin Proteins 0.000 description 2
- 102000006947 Histones Human genes 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 102000005348 Neuraminidase Human genes 0.000 description 2
- 108010006232 Neuraminidase Proteins 0.000 description 2
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 2
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 101710176177 Protein A56 Proteins 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- 239000000185 hemagglutinin Substances 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000000214 mouth Anatomy 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- -1 nitrite ions Chemical class 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000002823 phage display Methods 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 210000002845 virion Anatomy 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- JBDLMLZNDRLDIX-HJGDQZAQSA-N Asn-Thr-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O JBDLMLZNDRLDIX-HJGDQZAQSA-N 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000711450 Infectious bronchitis virus Species 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 101100028758 Influenza A virus (strain A/Swine/Wisconsin/1/1967 H1N1) PB1-F2 gene Proteins 0.000 description 1
- 229940124873 Influenza virus vaccine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- PVMPDMIKUVNOBD-CIUDSAMLSA-N Leu-Asp-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(O)=O PVMPDMIKUVNOBD-CIUDSAMLSA-N 0.000 description 1
- DZQMXBALGUHGJT-GUBZILKMSA-N Leu-Glu-Ala Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(O)=O DZQMXBALGUHGJT-GUBZILKMSA-N 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 101150103639 PB1 gene Proteins 0.000 description 1
- 239000008118 PEG 6000 Substances 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 108010067902 Peptide Library Proteins 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 239000006180 TBST buffer Substances 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 1
- 108091034135 Vault RNA Proteins 0.000 description 1
- CZCSLHYZEQSUNV-UHFFFAOYSA-N [Na].OB(O)O Chemical compound [Na].OB(O)O CZCSLHYZEQSUNV-UHFFFAOYSA-N 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 229910021505 gold(III) hydroxide Inorganic materials 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 238000012917 library technology Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 229910000027 potassium carbonate Inorganic materials 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
噬菌体编 | 插入的外援序列 |
1 | -L E A M D S N T L- |
3 | -G E M S D T L- |
5 | -I__A M D E T L- |
6 | -I E A M D S N T R- |
9 | -I A F__S N T L- |
11 | -_E T K R S N_L- |
12 | -A E R L D S_T_- |
13 | -I E A M D S N T R- |
16 | -K F A M I S L- |
一致序列 | -I E A M D S N T L- |
H9N2 | N I E A M D S N T L E |
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710152279.9A CN107064499B (zh) | 2017-03-15 | 2017-03-15 | 禽流感病毒np蛋白抗原表位多肽及其胶体金试纸盒 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710152279.9A CN107064499B (zh) | 2017-03-15 | 2017-03-15 | 禽流感病毒np蛋白抗原表位多肽及其胶体金试纸盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107064499A true CN107064499A (zh) | 2017-08-18 |
CN107064499B CN107064499B (zh) | 2019-06-18 |
Family
ID=59620405
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710152279.9A Active CN107064499B (zh) | 2017-03-15 | 2017-03-15 | 禽流感病毒np蛋白抗原表位多肽及其胶体金试纸盒 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107064499B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111551745A (zh) * | 2020-05-15 | 2020-08-18 | 安徽中起生物科技有限公司 | 禽流感病毒H7N9亚型N蛋白IgY抗体检测胶体金试纸及方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101591390A (zh) * | 2008-05-30 | 2009-12-02 | 中国科学院上海生命科学研究院 | 抗h5n1来源的禽流感病毒核蛋白np的单克隆抗体及其应用 |
JP2012052916A (ja) * | 2010-09-01 | 2012-03-15 | Fujirebio Inc | A型インフルエンザウイルス核タンパク質の第100番アミノ酸がアルギニンであるか否かを判定する方法 |
US20140234358A1 (en) * | 2009-04-03 | 2014-08-21 | Merial Limited | Newcastle disease virus vectored avian vaccines |
CN104582725A (zh) * | 2012-07-17 | 2015-04-29 | 梅里亚有限公司 | 减毒的猪流感疫苗及其制备和使用方法 |
CN106190993A (zh) * | 2015-04-02 | 2016-12-07 | 中国动物卫生与流行病学中心 | 禽流感疫苗和诊断抗原所用种毒的制备方法 |
CN106290867A (zh) * | 2016-08-17 | 2017-01-04 | 广东省农业科学院动物卫生研究所 | 一种同时检测五种禽垂直传播疾病抗体的液相芯片及方法 |
-
2017
- 2017-03-15 CN CN201710152279.9A patent/CN107064499B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101591390A (zh) * | 2008-05-30 | 2009-12-02 | 中国科学院上海生命科学研究院 | 抗h5n1来源的禽流感病毒核蛋白np的单克隆抗体及其应用 |
US20140234358A1 (en) * | 2009-04-03 | 2014-08-21 | Merial Limited | Newcastle disease virus vectored avian vaccines |
JP2012052916A (ja) * | 2010-09-01 | 2012-03-15 | Fujirebio Inc | A型インフルエンザウイルス核タンパク質の第100番アミノ酸がアルギニンであるか否かを判定する方法 |
CN104582725A (zh) * | 2012-07-17 | 2015-04-29 | 梅里亚有限公司 | 减毒的猪流感疫苗及其制备和使用方法 |
CN106190993A (zh) * | 2015-04-02 | 2016-12-07 | 中国动物卫生与流行病学中心 | 禽流感疫苗和诊断抗原所用种毒的制备方法 |
CN106290867A (zh) * | 2016-08-17 | 2017-01-04 | 广东省农业科学院动物卫生研究所 | 一种同时检测五种禽垂直传播疾病抗体的液相芯片及方法 |
Non-Patent Citations (2)
Title |
---|
未知: "获得码:WP_009258787", 《NCBI》 * |
胡思顺等: "H9N2型禽流感病毒和蛋白的原核表达及多克隆抗体制备", 《生物技术通讯》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111551745A (zh) * | 2020-05-15 | 2020-08-18 | 安徽中起生物科技有限公司 | 禽流感病毒H7N9亚型N蛋白IgY抗体检测胶体金试纸及方法 |
CN111551745B (zh) * | 2020-05-15 | 2023-04-07 | 安徽中起生物科技有限公司 | 禽流感病毒H7N9亚型N蛋白IgY抗体检测胶体金试纸及方法 |
Also Published As
Publication number | Publication date |
---|---|
CN107064499B (zh) | 2019-06-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Padilla-Quirarte et al. | Protective antibodies against influenza proteins | |
CA2617483C (en) | Modified influenza virus for monitoring and improving vaccine efficiency | |
CA2790949C (en) | Human monoclonal antibodies derived from human b cells and having neutralizing activity against influenza a viruses | |
Graves et al. | Preparation of influenza virus subviral particles lacking the HA1 subunit of hemagglutinin: unmasking of cross-reactive HA2 determinants | |
CN104007261B (zh) | 禽三种呼吸道疾病三联快速检测试剂盒及应用 | |
CN103665152B (zh) | 犬细小病毒单域抗体及其制备方法和应用 | |
CN102406931B (zh) | 大流行流感病毒裂解疫苗 | |
CN106668854A (zh) | 一种四价亚单位流感疫苗及其制备方法 | |
CN107344968A (zh) | 一种用于检测禽流感病毒h7n9的时间分辨荧光免疫分析方法 | |
CN110272473A (zh) | 甲型流感通用型病毒样颗粒及其制备方法和应用 | |
CN107201346A (zh) | 3b蛋白优势表位缺失的口蹄疫标记疫苗株及其构建方法和应用 | |
CN101879312B (zh) | 广谱型流感疫苗及其制备方法 | |
CN107064499B (zh) | 禽流感病毒np蛋白抗原表位多肽及其胶体金试纸盒 | |
CN115947835B (zh) | 靶向乙型流感病毒核蛋白的抗体及其应用 | |
CN104911150B (zh) | 一种h3n2犬流感病毒的单克隆抗体杂交瘤细胞株的建立及其单克隆抗体的制备与应用 | |
CN103030693B (zh) | 高致病性禽流感的中和分子及其制备方法 | |
CN104965083B (zh) | 一种检测h3n2亚型犬流感病毒的试剂盒 | |
CN106596931A (zh) | 一种广东烟区黄瓜花叶病毒胶体金快速检测试纸条 | |
CN109111507A (zh) | 病毒重组糖蛋白及其真核细胞高效表达方法与应用 | |
CA3026094A1 (en) | Modification of engineered influenza hemagglutinin polypeptides | |
CN103235129B (zh) | 马立克氏病病毒和j亚群禽白血病病毒快速联检试纸条 | |
CN115724959B (zh) | 靶向甲型流感病毒核蛋白的抗体及其应用 | |
CN115819566B (zh) | 靶向甲型流感病毒核蛋白的抗体及其应用 | |
CN109824775A (zh) | 口蹄疫a型武汉株单克隆抗体及其组合物与应用 | |
CN103397001B (zh) | 一种用于浓缩纯化流感病毒的制剂及方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: No. 85 Keyun Road, High tech Zone, Qingdao, Shandong Province, 266000 Patentee after: Qingdao Blue Animal Health Group Co.,Ltd. Patentee after: QINGDAO ANIMAL PROTECTION NATIONAL ENGINEERING TECHNOLOGY RESEARCH CENTER CO.,LTD. Patentee after: SHANDONG DELINORE BIO-ENGINEERING Co.,Ltd. Patentee after: QINGDAO VLAND BIOTECH Inc. Address before: No. 16 Tianhai Road, Hongdao Street, Chengyang District, Qingdao City, Shandong Province, 266114 Patentee before: QINGDAO VLAND BIOTECH GROUP CO.,LTD. Patentee before: QINGDAO ANIMAL PROTECTION NATIONAL ENGINEERING TECHNOLOGY RESEARCH CENTER CO.,LTD. Patentee before: SHANDONG DELINORE BIO-ENGINEERING Co.,Ltd. Patentee before: QINGDAO VLAND BIOTECH Inc. |
|
CP03 | Change of name, title or address |