CN107064208A - A kind of universal calorimetric biosensor - Google Patents
A kind of universal calorimetric biosensor Download PDFInfo
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- CN107064208A CN107064208A CN201710063802.0A CN201710063802A CN107064208A CN 107064208 A CN107064208 A CN 107064208A CN 201710063802 A CN201710063802 A CN 201710063802A CN 107064208 A CN107064208 A CN 107064208A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N25/00—Investigating or analyzing materials by the use of thermal means
- G01N25/20—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
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Abstract
The present invention provides a kind of universal calorimetric biosensor, it is adaptable to sheet and plate-like form heat sensor, including the adiabatic converging device of constant temperature sample introduction, thermally-stabilised microresponse device, small temp difference measurer, and is combined by snap joint.Many stream sample introductions are through the adiabatic converging device constant temperature of constant temperature sample introduction and collaborate, reacted in into thermally-stabilised microresponse device, thermometric is carried out through small temp difference measurer, system highly versatile, the need for adapting to many streams, many components, Complicated Flow and Operating condition adjustment, flexibility is high, heat endurance is good, simple and compact for structure, modularization, strong applicability, the on-line monitoring in the process such as enzyme analysis, biochemistry detection, environmental monitoring and biochemistry, fermentation can be applied, be particularly suitable for use in thermometric analysis and in-site measurement.
Description
Technical field
The present invention relates to a kind of universal calorimetric biosensor, it can apply in enzyme analysis, biochemistry detection, environment prison
The on-line monitoring of the process such as survey and biochemical, fermentation.
Background technology
The development of Measurement for Biochemistry, it is desirable to which various biochemical components are effectively analyzed, as, enzyme analysis, environmental monitoring,
Be also required in special component formed in on-line determination course of reaction or consumption, fermentation process to raw material and intermediate product and
The concentration METHOD FOR CONTINUOUS DETERMINATION of material etc. is generated to progress of control process etc..Because said process has nonlinearity and random
Property, by assuming that and the obtained mechanism of experiment and the application of Controlling model are all limited, it is difficult to accurately and effectively describe
The characteristic of whole process is, it is necessary to research and develop the analytical instrument for biochemical process.
Biochemical process chemical substance, biological substance are widely used to reference to the biology sensor of Flow Injection Analysis System
Analysis and detection.For the Site Detection and on-line monitoring of biochemical process, due to being to complete online, sample tends not to carry out
Complicated gives processing, it will usually include other materials, and what is had also has color.Electrochemical sensor is frequently subjected in same substrate electric
The interference of chemically reactive substance, optical interference material that is that photometric method was easily existed originally by reagent or being formed in the reaction
Influence, the two be applied to on-line checking exist limitation.Comparatively, calorimetric biosensor is applied to most of life
Change reaction, do not influenceed by sample properties such as color, muddy and electrochemistry, be particularly suitable for detecting undressed actual sample
Product, the specificity of calorimetry can be greatly enhanced by desmoenzyme etc..
The analysis of biochemical process often refers to multiple parameters, and need to monitor simultaneously, adapts to multicomponent and measures simultaneously and complicated
The need for flow（Enrichment, detection integrated calorimetric biosensor, ZL 200910126677.9；Based on reversible immobilised enzymes
Calorimetric biosensor, ZL 200910126676.4）, can be combined by universal calorimetric biosensor a variety of
Flow change is realized.
Patent（The hot bioreactor device of enzyme, ZL 201120033726.7）There is provided a kind of hot bioreactor dress of enzyme
Put, at least there is following technological deficiency：The need for not adapting to be enriched with, detecting the Complicated Flows such as integration and Operating condition adjustment；Only
Using immobilised enzymes, there is limitation；Enzyme post replacement operation is cumbersome, and system interference is big；Its main shunt mode, due to pressure,
The complicated factors such as flowing, it is more difficult to obtain the identical test of fluidised form, flow, concentration distribution and reference stream；Structure only with
Thermistor, needs complicated extra bridge circuit, exists non-linear；Heated constant temperature is only devised, and constant temperature pipeline is long, should not control
Flow injection distributed density；Structure is relative complex, not compact, immobilised enzymes preserve inconvenience, it is unsuitable scene and use online.
The content of the invention
Present invention aim to address the technological deficiency of existing calorimetric biosensor, there is provided a kind of universal calorimetric
Biology sensor, can apply the on-line monitoring in the process such as enzyme analysis, biochemistry detection, environmental monitoring and biochemistry, fermentation.
The technical solution adopted for the present invention to solve the technical problems is that a kind of universal calorimetric biosensor includes
Constant temperature sample introduction thermal insulation converging device, thermally-stabilised microresponse device, small temp difference measurer, adiabatic base, insulated case, positioning spiral shell
Bolt, clamp nut and heat-insulating cover, the adiabatic converging device of the constant temperature sample introduction realize the constant temperature and adiabatic condition of many stream sample introductions
Under interflow, the thermally-stabilised microresponse device is to maintain the microresponse device of heat endurance, and the small temp difference measurer is
Using tabular and the small temp difference measurer of sheet form heat sensor, the thermally-stabilised microresponse device is placed on the constant temperature
Between sample introduction thermal insulation converging device and the small temp difference measurer, respectively by the half of the adiabatic converging device of the constant temperature sample introduction
The end socket of the cylinder hemicone projection insertion thermally-stabilised reactor inlet and the semicolumn half cone of the small temp difference measurer
The end socket of the body projection insertion thermally-stabilised reactor outlet carries out closed combination, and the bolt is fixed on the adiabatic bottom
On seat, through the positioning hole of the small temp difference measurer, and the constant temperature sample introduction thermal insulation interflow as described in clamp nut elastic compression
Device, the thermally-stabilised microresponse device and the small temp difference measurer, the adiabatic converging device of the constant temperature sample introduction are fixed on
In adiabatic base, the insulated case is disposed with around the thermally-stabilised microresponse device and the small temp difference measurer, on
Portion is disposed with heat-insulating cover, inflow duct constant temperature of many stream sample introductions through the adiabatic converging device of the constant temperature sample introduction, through the constant temperature
The outflow duct interflow of sample introduction thermal insulation converging device, enters described thermally-stabilised small anti-through the thermally-stabilised microresponse device entrance
Answer and reacted in device, the outlet outflow through the thermally-stabilised microresponse device, the ostium through the small temp difference measurer
Road enters the small temp difference measurer and carries out thermometric, the outflow duct outflow through the small temp difference measurer, by setting
The adiabatic converging device of the constant temperature sample introduction of twinned structure, the thermally-stabilised microresponse device and the small temp difference measurer point
It Zuo Wei not measure and reference, be combined as one group of Measurement channel and reference channel, be such as multiplied simultaneously Measurement channel and reference are logical
Road, can be achieved to measure while multigroup passage.
Further, the adiabatic converging device of the constant temperature sample introduction is made up of thermostat, heat insulation taper cock and gland, institute
State thermostat to be made up of high chemically inert metal material, the thermostat carries out temperature control simultaneously by external heat/refrigerating plant
Constant temperature, the thermostat is provided with the taper consent coordinated with the heat insulation taper cock, and the heat insulation taper cock is by height
Chemically inert heat insulator is made, and the heat insulation taper cock is by gland elastic compression and is maintained in taper consent,
The taper consent side, which is provided with three tunnels, is used for the level inflow duct of sample introduction, and the heat insulation taper cock is provided with and taper consent
Three tunnels described in flow into and flow into duct described in three tunnels that duct coordinates, the central axis in duct is flowed into described in three tunnels by described
The axle center of heat insulation taper cock, inflow duct level stops at heat insulation taper cock axle center and vertical vertically described in three tunnels
Perforate is upwardly extended, as outflow duct, the length that duct is flowed into described in three tunnels is identical, and the level in the adjacent inflow duct
Angle is identical, is switched over by rotating the heat insulation taper cock, the inflow duct and the heat of the thermostat
The inflow duct of insulating conical cock is respectively communicated with and flows into duct, two tunnels inflow duct and three tunnels inflow duct all the way, real
Existing single current enters single exit case, double fluid and enters single exit case and three inflow single exit cases, and sample introduction flows through permanent during the inflow duct of thermostat
Temperature, then passes through the inflow duct of the heat insulation taper cock and collects outflow by flow pass interflow.
Further, the thermally-stabilised microresponse device, including reactor outer tube, reactor inner tube, support annulus, end socket
And filter screen, the reactor outer tube and the reactor inner tube are equal length, the pipe fitting of different bores, the reactor outer tube with
The axis of the reactor inner tube is overlapped and nested, is deposited between the outer wall of the inwall of the reactor outer tube and the reactor inner tube
In the air gap, the outer rim of the support annulus is concordant with the reactor outer tube outer wall face, it is described support annulus inner edge with
The reactor inner tube wall face is concordant, and the support annulus side is flat surface, and opposite side is provided with bulge loop, and the branch is held round
The bulge loop interference of ring is embedded in the air gap at the reactor outer tube and the reactor inner tube two ends, and the end socket is opened along axis
Hole is as the gateway of the disposable thermally-stabilised microresponse device, and filter screen described in the end socket disposed inboard is described
End socket interference is embedded in the reactor inner tube, and the reactor outer tube, reactor inner tube and support annulus are by high chemical inertness
Thermal inertia hard material be made, the end socket is made up of high chemically inert thermal inertia soft elastic material.
Further, small temp difference measurer by heat sensor A, heat sensor B, constant temperature is heat sink, temperature-sensitive cavity, heat insulation
Supporter, heat insulation gland, inflow duct and outflow duct composition, the heat sensor A and the heat sensor B are twin heat
Sensor, is the contact heat sensor of tabular and sheet form, preferably sheet thermoelectric pile, the heat sensor A and described
A heat sensor B side end face is hot junction face, and another side end face is cold end face, the heat sensor A and the heat sensor B's
Phase the same side end face and the constant temperature are heat sink to be thermally contacted good, and identical another side end face is the temperature-sensitive cavity, the temperature-sensitive chamber
Body connects the inflow duct and the outflow duct, the relative position guarantee flowing in the inflow duct and the outflow duct
To go out in tripping in, controllable temperature constant state can be achieved in built-in thermostat module that the constant temperature is heat sink, and it is truncated cone-shaped that the constant temperature is heat sink
The metal derby of formula, is processed into plane and is well thermally contacted with the heat sensor A and the heat sensor B respectively sideways, described
Pit is provided with heat insulation supporter, the built-in heat sensor A, the heat sensor B and the constant temperature are heat sink, the heat is absolutely
The heat insulation gland is placed on edge supporter, it is ensured that the heat sensor A, the heat sensor B and the constant temperature it is heat sink with
Environment heat insulation, the heat sensor A and the heat sensor B signal output are concatenated according to difference output mode, the heat
Insulation support body and the heat insulation gland are made by high chemically inert heat insulator.
The beneficial effects of the invention are as follows highly versatile can use immobilised enzymes, liquid enzyme etc., adapt to enrichment, detection one
The need for many streams, many components, Complicated Flow and the Operating condition adjustments such as change；Flexibility is high, it is possible to achieve single current enters single exit case, double fluid
Enter single exit case and three and flow into single exit case change, different size, the reactor of specification are convenient for changing adjustment；It is different from shunt mode,
Twin parallel stream mode is easier to obtain the identical test of fluidised form, flow, concentration distribution and reference stream；Devise heating/
Refrigerated constant temperature, can be to be measured using refrigeration protection bioactivity in test gap in addition to work carries out temperature control；Heat endurance is good,
Adapt to thermometric analysis；The simple compact, modularization of system architecture, reactor changes convenient, strong applicability, and the scene of being particularly suitable for use in is surveyed
Amount.
Brief description of the drawings
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is universal calorimetric biosensor structural representation.
Wherein：1st, the adiabatic converging device of constant temperature sample introduction, 2, thermally-stabilised microresponse device, 3, small temp difference measurer, 4, absolutely
Hot base, 5, insulated case, 6, bolt, 7, clamp nut, 8, semiconductor refrigerating/heater element, 9, heat exchanger, 10, wind
Fan, 11, spring, 12, heat-insulating cover.
Fig. 2 is the structural representation of the adiabatic converging device of constant temperature sample introduction.
Fig. 3 is the structural representation of heat insulation taper cock.
Wherein：101st, thermostat, 102, heat insulation taper cock, 103, taper consent, 104, gland, 105, ostium
Road, 106, outflow duct.
Fig. 4 is that single current enters the principle schematic that single exit case, double fluid enter single exit case and three inflow single exit cases.
Fig. 5 is the structural representation of thermally-stabilised microresponse device.
Wherein：201st, reactor outer tube, 202, reactor inner tube, 203, support annulus, 204, end socket, 205, filter screen.
Fig. 6 is the structural representation of small temp difference measurer.
Wherein：301st, heat sensor A, 302, heat sensor B, 303, constant temperature is heat sink, 304, temperature-sensitive cavity, 305, heat insulation
Supporter, 306, heat insulation gland, 307, flow into duct, 308, outflow duct.
Embodiment
The present invention combine nationality by below in conjunction with embodiment illustrate the content of the present invention, the model that is not intended to limit the present invention
Enclose.
As shown in figure 1, a kind of universal calorimetric biosensor include the adiabatic converging device 1 of constant temperature sample introduction, it is thermally-stabilised
Microresponse device 2, small temp difference measurer 3, adiabatic base 4, insulated case 5, bolt 6, clamp nut 7, semiconductor system
Cold/heater element 8, heat exchanger 9, fan 10, spring 11 and heat-insulating cover 12, constant temperature sample introduction thermal insulation converging device 1 realize many streams
Interflow under the constant temperature and adiabatic condition of sample introduction, thermally-stabilised microresponse device 2 is to maintain the microresponse device of heat endurance, small
Temp difference measurer 3 is the small temp difference measurer using column, pearl and spherical heat sensor, thermally-stabilised microresponse device 2
It is placed between the adiabatic converging device 1 of constant temperature sample introduction and small temp difference measurer 3, respectively by the adiabatic converging device of constant temperature sample introduction
The end socket and the semicolumn hemicone of small temp difference measurer 3 of the 1 thermally-stabilised entrance of reactor 2 of semicolumn hemicone projection insertion
The end socket that the thermally-stabilised reactor 2 of projection insertion is exported carries out closed combination, and bolt 6 is fixed on adiabatic base 4, through small
The positioning hole of temp difference measurer 3, and it is steady by clamp nut 7 and the adiabatic converging device 1 of the elastic compression constant temperature sample introduction of spring 11, heat
Determine microresponse device 2 and small temp difference measurer 3, constant temperature sample introduction thermal insulation converging device 1 is fixed in adiabatic base 4, and constant temperature enters
The bottom of sample thermal insulation converging device 1 sets semiconductor refrigerating/heater element 8, heat exchanger 9 and fan 10 to carry out temperature control, adiabatic bottom
The bottom skirt portion perforate of seat 4 or Partial Resection, for the good heat exchange between heat exchanger and environment, it is ensured that semiconductor refrigerating/heating
The normal work of device 8.Insulated case 5, top arrangement are disposed with around thermally-stabilised microresponse device 2 and small temp difference measurer 3
There is heat-insulating cover 12, inflow duct constant temperature of many stream sample introductions through the adiabatic converging device 1 of constant temperature sample introduction collaborates through the thermal insulation of constant temperature sample introduction
The outflow duct interflow of device 1, enters through the thermally-stabilised entrance of microresponse device 2 and is reacted in thermally-stabilised microresponse device 2, passed through
The outlet outflow of thermally-stabilised microresponse device 2, the inflow duct through small temp difference measurer 3 is entered into small temp difference measurer 3
Row thermometric, the outflow duct outflow through small temp difference measurer 3, by the adiabatic converging device of constant temperature sample introduction for setting twinned structure
1st, thermally-stabilised microresponse device 2 and small temp difference measurer 3 respectively as measurement and reference, its medium and small temp difference measurer 3
The signal output of twin heat sensor is concatenated according to difference output mode, is combined as one group of Measurement channel and reference channel, as
When be multiplied Measurement channel and reference channel, can be achieved to measure while multigroup passage.
As shown in Figures 2 and 3, the adiabatic converging device 1 of constant temperature sample introduction is by thermostat 101, heat insulation taper cock 102 and pressure
Lid 104 is constituted, and thermostat 101 is polygon stainless steel metal block, and the bottom of thermostat 101 is filled by semiconductor heating/refrigerating
Progress temperature control and constant temperature are put, thermostat 101 is provided with the taper consent 103 coordinated with heat insulation taper cock 102, heat insulation taper
Cock 102 is compressed and is maintained in taper consent 103, the top cloth of heat insulation taper cock 102 by spring force by gland 104
The projection of semicolumn hemicone is equipped with, the end socket for inserting microresponse device entrance, it is ensured that runner is closed.Taper consent 103
Side, which is provided with three tunnels, is used for the inflow duct 105 of sample introduction, and heat insulation taper cock 102 is provided with the three tunnels inflow with taper consent 103
Three roads that duct 105 coordinates flow into duct 105, and the central axis that three roads flow into duct 105 passes through heat insulation taper cock 2
Axle center, the length that three roads flow into duct 105 is identical, and the adjacent horizontal sextant angle of inflow duct 105 is 45 °, and three roads flow into duct 105
Level stops at the axle center of heat insulation taper cock 102 and extends vertically upwards perforate vertically, is used as outflow duct 106.It is hot exhausted
Edge taper cock 102 and gland 104 are by polytetrafluoroethylene (PTFE)（Hydrophilic modification）Material is made.As shown in figure 4, heat insulation taper
45 ° and 90 ° of the anglec of rotation of difference that cock 102 is counterclockwise, three tunnels of thermostat 101 flow into duct 105 and heat insulation taper cock
102 three roads flow into duct 105 and connect inflow duct 105, the two tunnels inflow tunnel of duct 105 and three inflow duct 105 all the way, realize
Three flow into single exit case（Fig. 4 a）, double fluid enter single exit case（Fig. 4 b）Enter single exit case with single current（Fig. 4 c）, sample introduction flows through the stream of thermostat 1
By constant temperature when entering duct 105, three roads for then passing through heat insulation taper cock 102 flow into duct 105 and by flow pass 106
Collect outflow in interflow.Heat insulation taper cock 102 turns clockwise and can also realize above-mentioned functions, and principle is consistent.
As shown in figure 5, thermally-stabilised microresponse device 2 includes reactor outer tube 201, reactor inner tube 202, support annulus
203rd, end socket 204, filter screen 205 and sealing-plug 206, reactor outer tube 201 and reactor inner tube 202 are equal length, different bores
Plastic conduit, its wall thickness is about 1mm, and reactor outer tube 201 is overlapped and nested with the axis of reactor inner tube 202, reaction
There is the air gap between the inwall of device outer tube 201 and the outer wall of reactor inner tube 202, the air gap is about 1mm, support annulus
203 be the annulus that hard plastics are made, and supports the outer rim of annulus 203 concordant with the outside wall surface of reactor outer tube 201, support
The inner edge of annulus 203 is concordant with the internal face of reactor inner tube 202, and support annulus 203 is provided with bulge loop high height about 2mm, branch
Bulge loop interference insertion reaction device outer tube 201 and the air gap at the two ends of reactor inner tube 202 of ring 203 are held round, end socket 204 is by bullet
Property elastomeric material is made, and end socket 204 is along gateway of the axis perforate as microresponse device, the insertion reaction device inner tube of end socket 204
202, the disposed inboard filter screen 205 of the inside end socket 204 of reactor inner tube 202.
As shown in fig. 6, small temp difference measurer 3 is by heat sensor A301, heat sensor B302, constant temperature is heat sink 303, temperature-sensitive
Cavity 304, heat insulation supporter 305, heat insulation gland 306 flow into duct 307 and outflow duct 308 and constituted, heat sensor
A301 and heat sensor B302 are twin heat sensors, and from the consistent sheet thermoelectric pile of performance structure, heat insulation is supported on 305
Pit is provided with, for built-in heat sensor A301, heat sensor B302 and constant temperature heat sink 303, heat sensor A301 and heat are sensed
Device B302 hot junction face is in close contact with the side of constant temperature heat sink 303, and smears heat-conducting silicone grease reduction thermal contact resistance, heat sensor
A301 and heat sensor B302 cold end face is respectively overlay in the temperature-sensitive cavity 304 that pit is outputed, and is directly bonded to heat insulation
In support 305, heat sensor A301, heat sensor B302 are directly contacted with the reaction solution in temperature-sensitive cavity 304 during measurement, sense
Warm cavity 304 is the circular cylindrical cavity that thickness is 2-5mm, and lateral opening hole connection flows into duct 307 and outflow duct 308, and heat is absolutely
Edge supports 305 lower disposeds to have the projection of semicolumn hemicone, along raised axle center perforate as duct 307 is flowed into, flows out duct 8
305 horizontal bores are supported to obtain in heat insulation, relative position is higher than inflow duct 307, it is ensured that flow to go out in tripping in, constant temperature heating
Heavy 303 be the metal derby of round platform form, and upward, controllable constant temperature shape can be achieved in embedded semiconductor refrigerating/heating module for its bottom surface
State, constant temperature is heat sink, and 303 sides are processed into plane and are in close contact respectively with heat sensor A301 and heat sensor B302, heat insulation
Place heat insulation gland 306 in the top of supporter 305, it is ensured that heat sensor A301, heat sensor B302 and constant temperature heat sink 303 and ring
Border heat insulation, heat sensor A301 and heat sensor B302 signal output are concatenated according to difference output mode.Heat insulation is supported
Body 305 and heat insulation gland 306 are by polytetrafluoroethylene (PTFE)（Hydrophilic modification）It is made.
A kind of universal calorimetric biosensor proposed by the present invention can be biological as enrichment, detection integrated calorimetric formula
Sensor flow is used to detect heavy metal, and idiographic flow and detection method are referring to document（Novel and multifunctional calorimetric bio-sensing
Device system research and exploitation, Chinese journal of scientific instrument, 2011:32(12), 45-51）, by the present invention and buffer solution, sample, wriggling
Pump etc. constitutes flow injection system, realizes enrichment, the testing process of detection integration, and the thermally-stabilised inside of microresponse device 2 is full of
113 resins of 40 mesh, and by combining the raised introducing system stream of semicolumn hemicone of the present invention, surrounding places insulated case
5, and the clamp nut 7 on bolt 6 is screwed, then tested according to flow, the heat letter that small temp difference measurer 3 is exported
Number by microcomputer detection process program complete, the amount of last printed sample.
Claims (1)
1. a kind of universal calorimetric biosensor includes the adiabatic converging device of constant temperature sample introduction（1）, thermally-stabilised microresponse device
（2）, small temp difference measurer（3）, adiabatic base（4）, insulated case（5）, bolt（6）, clamp nut（7）And heat-insulating cover
（12）, it is characterised in that：
The thermally-stabilised microresponse device（2）It is placed on the adiabatic converging device of the constant temperature sample introduction（1）With the small differential temperature survey
Device（3）Between, respectively by the adiabatic converging device of the constant temperature sample introduction（1）The semicolumn hemicone projection insertion heat it is steady
Determine reactor（2）The end socket of entrance and the small temp difference measurer（3）Semicolumn hemicone projection insertion it is described thermally-stabilised
Reactor（2）The end socket of outlet carries out closed combination, the bolt（6）It is fixed on the adiabatic base（4）On, through institute
State small temp difference measurer（3）Positioning hole, and by clamp nut（7）Constant temperature sample introduction thermal insulation converging device described in elastic compression
（1）, the thermally-stabilised microresponse device（2）With the small temp difference measurer（3）, the adiabatic converging device of the constant temperature sample introduction
（1）It is fixed on adiabatic base（4）It is interior, the thermally-stabilised microresponse device（2）With the small temp difference measurer（3）Surrounding is arranged
There is the insulated case（5）, top is disposed with heat-insulating cover（12）, by setting the constant temperature sample introduction thermal insulation of twinned structure to collaborate
Device（1）, the thermally-stabilised microresponse device（2）With the small temp difference measurer（3）Respectively as measurement and reference, combination
For one group of Measurement channel and reference channel, be such as multiplied Measurement channel and reference channel simultaneously, and the same of multigroup passage can be achieved
When measure；
The adiabatic converging device of the constant temperature sample introduction（1）By thermostat（101）, heat insulation taper cock（102）And gland（104）Group
Into the thermostat（101）It is provided with and the heat insulation taper cock（102）The taper consent of cooperation（103）, the heat insulation
Taper cock（102）By gland（104）Elastic compression is simultaneously maintained at taper consent（103）It is interior, the taper consent（103）Open side
The level that having three tunnels is used for sample introduction flows into duct（105）, the heat insulation taper cock（102）It is provided with and taper consent（103）
Three tunnels described in flow into duct（105）Duct is flowed into described in three tunnels coordinated（105）, duct is flowed into described in three tunnels（105）In
Heart axis passes through the heat insulation taper cock（102）Axle center, flow into duct described in three tunnels（105）Level stops at hot exhausted
Edge taper cock（102）Axle center simultaneously extends vertically upwards perforate vertically, is used as outflow duct（106）, ostium described in three tunnels
Road（105）Length it is identical, and the adjacent inflow duct（105）Horizontal sextant angle it is identical, bored by rotating the heat insulation
Shape cock（102）Switch over, the thermostat（101）The inflow duct（105）With the heat insulation taper cock
The inflow duct（105）It is respectively communicated with and flows into duct, two tunnels inflow duct and three tunnels inflow duct all the way, realizes that single current enters list
Outflow, double fluid enter single exit case and three and flow into single exit case；
The thermally-stabilised microresponse device（2）, including reactor outer tube（201）, reactor inner tube（202）, support annulus
（203）, end socket（204）And filter screen（205）, the reactor outer tube（201）With the reactor inner tube（202）For equal length,
The pipe fitting of different bores, the reactor outer tube（201）With the reactor inner tube（202）Axis overlap and nested, it is described
Reactor outer tube（201）Inwall and the reactor inner tube（202）Outer wall between there is the air gap, the support annulus
（203）Outer rim and the reactor outer tube（201）Outside wall surface is concordant, the support annulus（203）Inner edge and the reaction
Device inner tube（202）Internal face is concordant, the support annulus（203）Side is flat surface, and opposite side is provided with bulge loop, the support
Annulus（203）Bulge loop interference be embedded in the reactor outer tube（201）With the reactor inner tube（202）Between the air at two ends
Gap, the end socket（204）Along import and export of the axis perforate as the disposable thermally-stabilised microresponse device, the envelope
Head（204）Filter screen described in disposed inboard（205）, the end socket（204）Interference is embedded in the reactor inner tube（202）, it is described anti-
Answer device outer tube（201）, reactor inner tube（202）, support annulus（203）And sealing-plug（206）It is lazy by the chemically inert heat of height
Property rigid plastics is made, the end socket（204）It is made up of high chemically inert thermal inertia softer, elastic rubber；
The small temp difference measurer（3）, by heat sensor A（301）, heat sensor B（302）, constant temperature is heat sink（303）, temperature-sensitive
Cavity（304）, heat insulation supporter（305）, heat insulation gland（306）, flow into duct（307）With outflow duct（308）Composition,
The heat sensor A（301）With the heat sensor B（302）It is twin heat sensor, is tabular and the contact of sheet form
Heat sensor, the heat sensor A（301）With the heat sensor B（302）Phase the same side end face and the constant temperature it is heat sink
（303）Thermo-contact is good, and identical another side end face is the temperature-sensitive cavity（304）, the temperature-sensitive cavity（304）The connection stream
Enter duct（307）With the outflow duct（308）, the inflow duct（307）With the outflow duct（308）Relative position
Ensure flowing to go out in tripping in, the constant temperature is heat sink（303）For the metal derby of round platform form, be processed into sideways plane and respectively with
The heat sensor A（301）With the heat sensor B（302）Good thermo-contact, the heat insulation supporter（305）On be provided with
Pit, the built-in heat sensor A（301）, the heat sensor B（302）It is heat sink with the constant temperature（303）, the heat insulation
Supporter（305）It is upper to place the heat insulation gland（306）, the heat sensor A（301）With the heat sensor B（302）'s
Signal output is concatenated according to difference output mode.
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| CN201710063802.0A CN107064208B (en) | 2017-02-04 | 2017-02-04 | A kind of universal calorimetric biosensor |
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| CN201710063802.0A CN107064208B (en) | 2017-02-04 | 2017-02-04 | A kind of universal calorimetric biosensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490025A (en) * | 2018-03-30 | 2018-09-04 | 沈阳化工研究院有限公司 | A kind of the calorimetric test method and device of continuous flow gas phase reaction process |
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| EP0954748A1 (en) * | 1997-11-14 | 1999-11-10 | Motorola, Inc. | Sensor housing for a calorimetric gas sensor |
| CN201340401Y (en) * | 2009-01-06 | 2009-11-04 | 上海理工大学 | Detection device used for general-purpose pesticide residue rapid detecting biosensor |
| CN101614687A (en) * | 2009-03-06 | 2009-12-30 | 青岛大学 | Enrichment, detection integrated calorimetric biosensor |
| CN104655671A (en) * | 2014-12-31 | 2015-05-27 | 威仕英索(北京)仪器设备有限公司 | Adiabatic accelerating rate calorimeter and detection method thereof |
| JP2015200611A (en) * | 2014-04-10 | 2015-11-12 | 学校法人東北学院 | Calorimetric biosensor |
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2017
- 2017-02-04 CN CN201710063802.0A patent/CN107064208B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0954748A1 (en) * | 1997-11-14 | 1999-11-10 | Motorola, Inc. | Sensor housing for a calorimetric gas sensor |
| CN201340401Y (en) * | 2009-01-06 | 2009-11-04 | 上海理工大学 | Detection device used for general-purpose pesticide residue rapid detecting biosensor |
| CN101614687A (en) * | 2009-03-06 | 2009-12-30 | 青岛大学 | Enrichment, detection integrated calorimetric biosensor |
| JP2015200611A (en) * | 2014-04-10 | 2015-11-12 | 学校法人東北学院 | Calorimetric biosensor |
| CN104655671A (en) * | 2014-12-31 | 2015-05-27 | 威仕英索(北京)仪器设备有限公司 | Adiabatic accelerating rate calorimeter and detection method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490025A (en) * | 2018-03-30 | 2018-09-04 | 沈阳化工研究院有限公司 | A kind of the calorimetric test method and device of continuous flow gas phase reaction process |
| CN108490025B (en) * | 2018-03-30 | 2023-08-08 | 沈阳化工研究院有限公司 | Calorimetric test method and device for continuous flow gas phase reaction process |
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