CN107058435A - One kind method for extracting proteins from Cottonseed Meal - Google Patents
One kind method for extracting proteins from Cottonseed Meal Download PDFInfo
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- CN107058435A CN107058435A CN201710180188.6A CN201710180188A CN107058435A CN 107058435 A CN107058435 A CN 107058435A CN 201710180188 A CN201710180188 A CN 201710180188A CN 107058435 A CN107058435 A CN 107058435A
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- cottonseed meal
- filtrate
- solution
- kind method
- extracting proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The present invention provides one kind method for extracting proteins from Cottonseed Meal, and its step includes：A, Cottonseed Meal is crushed after be watered mixing, be centrifuged；B, filtering solution, remove residue, then carry out alkali soluble, and add protease, and heating maintains the temperature at 40 50 DEG C, and solution ph is 7.5 8.0, and the heat time is in 3 5h；C, temperature is quickly ramped up to 80 90 DEG C, carries out stopping heating after sterilization enzyme inactivation processing；D, sodium chloride is added into solution again, after stirring, solution is concentrated, dried, you can the protein in Cottonseed Meal is obtained.The present invention makes full use of resource, by the Protein Extraction of the high nutritive value contained in Cottonseed Meal, and the lipidated protein of extraction is high, and impurity is few, the residual without industrial abstract additive, and manufacture craft is simple, and with low cost but value height is adapted to industrialization large-scale production.
Description
Technical field
The invention belongs to cottonseed processing technical field, and in particular to one kind method for extracting proteins from Cottonseed Meal.
Background technology
China is Chan Mian big countries, accounts for more than the 60% of World cotton total output, and output of cotton in 2008 is 2052.6 ten thousand tons,
11,490,000 tons of cottonseed is produced, extracts and 6,430,000 tons of Cottonseed Meal is produced after grease, wherein containing 3,200,000 tons or so of protein, for many years
Come, the Cottonseed Meal after cottonseed oil 5% or so is produced with cottonseed and is used as animal feed, more than 95% as Fertilizer application, wastes
Substantial amounts of protein.
Protein content is generally 14-17% in cottonseed, can reach 29.7%, and its potency is close with animal protein, is
A kind of good protein.Cottonseed protein amino acid content enriches, and 18 kinds of amino acid contents are reasonable, close to FAO (Food and Agriculture Organization of the United Nation)
With standard as defined in the World Health Organization, they are albumin, globulin, prolamin and glutelin, and cottonseed egg is accounted for respectively
White total amount 6.81%, 17.57%, 5.33% and 70.11%.
The cottonseed resource of China is enriched very much, but the depth currently developed is also far from enough, is limited only to primary
The making of food, added value of product is too low.Deep processing, product of the exploitation with high technology content, ability are only carried out to cottonseed
This valuable resource is allowed to bring bigger interests for us.
Chinese patent CN101785527A " a kind of preparation method of dephenolization cottonseed protein and raffinose " is to egg in Cottonseed Meal
White and sugared extracts the method for all using organic solvent, and it is very thorough that its gossypol takes off, but it can also be brought into organic solvent
In albumen and sugar, and its method is comparatively laborious, complexity, and efficiency and purity is not also high, is not suitable for the industrial production of scale,
Therefore a kind of method for extracting protein preferably prepared in cottonseed is needed.
The content of the invention
It is an object of the invention to provide one kind from Cottonseed Meal method for extracting proteins, the present invention makes full use of resource,
By the Protein Extraction of the high nutritive value contained in Cottonseed Meal, the lipidated protein of extraction is high, and impurity is few.
The invention provides following technical scheme：
One kind method for extracting proteins from Cottonseed Meal, comprises the following steps：
A, Cottonseed Meal is crushed after be watered mixing, the weight part ratio of Cottonseed Meal and water is 1:15, it is centrifuged；
B, filtering solution, remove residue, then filtrate is carried out into alkali soluble, and add the weight part ratio of protease, protease and filtrate
For 1:20, heat filtrate and stir, maintain the temperature at 40-50 DEG C, solution ph is in 7.5-8.0, and the heat time is in 3-5h, stirring
Speed is in 60-80r/min；
C, step b residues are further washed, then individually carry out step b operations, obtained filtrate mixes with step b filtrate；
D, above-mentioned mixed filtrate temperature is quickly ramped up to 80-90 DEG C, carries out stopping heating after sterilization enzyme inactivation 5-10min, then
Secondary filtering residue；
The weight part ratio of e, the sodium chloride solution that addition concentration is 8% into filtrate again, filtrate and sodium chloride solution is 1:2, stirring
After uniform, filtrate is concentrated, dried, you can the protein in cottonseed is obtained.
It is preferred that, the step b protease is papain or cathepsin, and the protease is with low cost, and enzyme
Efficiency high is solved, is conducive to improving the recovery rate of protein.
It is preferred that, the reagent of the step b alkali solubles addition is buck, and its cost is low, and alkalescence is moderate, is conducive to controlling solution
PH value.
It is preferred that, the step b solution ph is the enzymolysis efficiency highest of protease under 7.5, the pH environment.
It is preferred that, the step b heat times are 5h, fully improve the utilization rate of material, make each composition reaction complete.
It is preferred that, the step e uses vacuum concentration method, and this method is simple, easy to operate, and thickening efficiency is high.
It is preferred that, the step e is concentrated into solution water content in 40-50%, it is to avoid the loss of protein, maximizes and ensures
The extracted amount of protein.
It is preferred that, the step e is conducive to the composition of protected protein matter not using the method being spray-dried, the drying means
It is destroyed, and drying efficiency is high.
The beneficial effects of the invention are as follows：
The present invention makes full use of resource, and by the Protein Extraction of the high nutritive value contained in Cottonseed Meal, the protein of extraction is pure
Degree is high, and impurity is few, the residual without industrial abstract additive.
Manufacture craft of the present invention is simple, and secondary pollution will not be produced in production process, and producing efficiency is high, flow is short, into
This is cheap but value is high, is adapted to industrialization large-scale production.
In manufacturing process of the present invention, while raising extraction rate of protein is maximized, saving water resource, heating evaporation
Moisture can be recycled, clean manufacturing, meet the requirement of social Green Development, be conducive to promoting the sustainable development of society
Exhibition, while also promoting the innovation ability of the industry, improves the defect in current industry.
Embodiment
Embodiment 1
One kind method for extracting proteins from Cottonseed Meal, comprises the following steps：
A, Cottonseed Meal is crushed after be watered mixing, the weight part ratio of Cottonseed Meal and water is 1:15, it is centrifuged；
B, filtering solution, remove residue, then filtrate is carried out into alkali soluble, and add the weight part ratio of protease, protease and filtrate
For 1:20, heat filtrate and stir, maintain the temperature at 40 DEG C, solution ph is 7.5, and the heat time exists in 5h, stir speed (S.S.)
80r/min；
C, step b residues are further washed, then individually carry out step b operations, obtained filtrate mixes with step b filtrate；
D, above-mentioned mixed filtrate temperature is quickly ramped up to 80 DEG C, carries out stopping heating after sterilization enzyme inactivation 10min, filter again
Residue；
The weight part ratio of e, the sodium chloride solution that addition concentration is 8% into filtrate again, filtrate and sodium chloride solution is 1:2, stirring
After uniform, filtrate is concentrated, dried, you can the protein in cottonseed is obtained.
Step b protease is papain, and the protease is with low cost, and enzymolysis efficiency is high, is conducive to improving albumen
The recovery rate of matter.
The reagent of step b alkali solubles addition is buck, and its cost is low, and alkalescence is moderate, is conducive to controlling the pH value of solution.
Step b solution ph is the enzymolysis efficiency highest of protease under 7.5, the pH environment.
The step b heat times are 5h, fully improve the utilization rate of material, make each composition reaction complete.
Step e uses vacuum concentration method, and this method is simple, easy to operate, and thickening efficiency is high.
Step e is concentrated into solution water content 50%, it is to avoid the loss of protein, maximizes the extracted amount for ensureing protein.
Step e is using the method for spray drying, and the drying means is conducive to the composition of protected protein matter not to be destroyed, and does
Dry efficiency high.
Embodiment 2
One kind method for extracting proteins from Cottonseed Meal, comprises the following steps：
A, Cottonseed Meal is crushed after be watered mixing, the weight part ratio of Cottonseed Meal and water is 1:15, it is centrifuged；
B, filtering solution, remove residue, then filtrate is carried out into alkali soluble, and add the weight part ratio of protease, protease and filtrate
For 1:20, heat filtrate and stir, maintain the temperature at 45 DEG C, solution ph is 7.5, and the heat time exists in 5h, stir speed (S.S.)
68r/min；
C, step b residues are further washed, then individually carry out step b operations, obtained filtrate mixes with step b filtrate；
D, above-mentioned mixed filtrate temperature is quickly ramped up to 89 DEG C, carries out stopping heating after sterilization enzyme inactivation 9min, filter again
Residue；
The weight part ratio of e, the sodium chloride solution that addition concentration is 8% into filtrate again, filtrate and sodium chloride solution is 1:2, stirring
After uniform, filtrate is concentrated, dried, you can the protein in cottonseed is obtained.
Step b protease is cathepsin, and the protease is with low cost, and enzymolysis efficiency is high, is conducive to improving albumen
The recovery rate of matter.
The reagent of step b alkali solubles addition is buck, and its cost is low, and alkalescence is moderate, is conducive to controlling the pH value of solution.
Step b solution ph is the enzymolysis efficiency highest of protease under 7.5, the pH environment.
The step b heat times are 5h, fully improve the utilization rate of material, make each composition reaction complete.
Step e uses vacuum concentration method, and this method is simple, easy to operate, and thickening efficiency is high.
Step e is concentrated into solution water content 45%, it is to avoid the loss of protein, maximizes the extracted amount for ensureing protein.
Step e is using the method for spray drying, and the drying means is conducive to the composition of protected protein matter not to be destroyed, and does
Dry efficiency high.
Embodiment 3
One kind method for extracting proteins from Cottonseed Meal, comprises the following steps：
A, Cottonseed Meal is crushed after be watered mixing, the weight part ratio of Cottonseed Meal and water is 1:15, it is centrifuged；
B, filtering solution, remove residue, then filtrate is carried out into alkali soluble, and add the weight part ratio of protease, protease and filtrate
For 1:20, heat filtrate and stir, maintain the temperature at 50 DEG C, solution ph is 7.5, and the heat time exists in 5h, stir speed (S.S.)
70r/min；
C, step b residues are further washed, then individually carry out step b operations, obtained filtrate mixes with step b filtrate；
D, above-mentioned mixed filtrate temperature is quickly ramped up to 90 DEG C, carries out stopping heating after sterilization enzyme inactivation 5min, filter again
Residue；
The weight part ratio of e, the sodium chloride solution that addition concentration is 8% into filtrate again, filtrate and sodium chloride solution is 1:2, stirring
After uniform, filtrate is concentrated, dried, you can the protein in cottonseed is obtained.
Step b protease is cathepsin, and the protease is with low cost, and enzymolysis efficiency is high, is conducive to improving albumen
The recovery rate of matter.
The reagent of step b alkali solubles addition is buck, and its cost is low, and alkalescence is moderate, is conducive to controlling the pH value of solution.
Step b solution ph is the enzymolysis efficiency highest of protease under 7.5, the pH environment.
The step b heat times are 5h, fully improve the utilization rate of material, make each composition reaction complete.
Step e uses vacuum concentration method, and this method is simple, easy to operate, and thickening efficiency is high.
Step e is concentrated into solution water content 48%, it is to avoid the loss of protein, maximizes the extracted amount for ensureing protein.
Step e is using the method for spray drying, and the drying means is conducive to the composition of protected protein matter not to be destroyed, and does
Dry efficiency high.
Embodiment 4
One kind method for extracting proteins from Cottonseed Meal, comprises the following steps：
A, Cottonseed Meal is crushed after be watered mixing, the weight part ratio of Cottonseed Meal and water is 1:15, it is centrifuged；
B, filtering solution, remove residue, then filtrate is carried out into alkali soluble, and add the weight part ratio of protease, protease and filtrate
For 1:20, heat filtrate and stir, maintain the temperature at 40 DEG C, solution ph is 8.0, and the heat time exists in 5h, stir speed (S.S.)
70r/min；
C, step b residues are further washed, then individually carry out step b operations, obtained filtrate mixes with step b filtrate；
D, above-mentioned mixed filtrate temperature is quickly ramped up to 85 DEG C, carries out stopping heating after sterilization enzyme inactivation 7min, filter again
Residue；
The weight part ratio of e, the sodium chloride solution that addition concentration is 8% into filtrate again, filtrate and sodium chloride solution is 1:2, stirring
After uniform, filtrate is concentrated, dried, you can the protein in cottonseed is obtained.
Step b protease is papain, and the protease is with low cost, and enzymolysis efficiency is high, is conducive to improving albumen
The recovery rate of matter.
The reagent of step b step b alkali solubles addition is buck, and its cost is low, and alkalescence is moderate, is conducive to controlling the pH of solution
Value.
The step b heat times are 5h, fully improve the utilization rate of material, make each composition reaction complete.
Step e uses vacuum concentration method, and this method is simple, easy to operate, and thickening efficiency is high.
Step e is concentrated into solution water content 50%, it is to avoid the loss of protein, maximizes the extracted amount for ensureing protein.
Step e is using the method for spray drying, and the drying means is conducive to the composition of protected protein matter not to be destroyed, and does
Dry efficiency high.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, although with reference to foregoing reality
Apply example the present invention is described in detail, for those skilled in the art, it still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic.All essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (8)
1. one kind method for extracting proteins from Cottonseed Meal, it is characterised in that comprise the following steps：
A, Cottonseed Meal is crushed after be watered mixing, the weight part ratio of Cottonseed Meal and water is 1:15, it is centrifuged；
B, filtering solution, remove residue, then filtrate is carried out into alkali soluble, and add the weight part ratio of protease, protease and filtrate
For 1:20, heat filtrate and stir, maintain the temperature at 40-50 DEG C, solution ph is in 7.5-8.0, and the heat time is in 3-5h, stirring
Speed is in 60-80r/min；
C, step b residues are further washed, then carry out independent step b operations, obtained filtrate mixes with step b filtrate；
D, above-mentioned mixed filtrate temperature is quickly ramped up to 80-90 DEG C, carries out stopping heating after sterilization enzyme inactivation 5-10min, then
Secondary filtering residue；
The weight part ratio of e, the sodium chloride solution that addition concentration is 8% into filtrate again, filtrate and sodium chloride solution is 1:2, stirring
After uniform, filtrate is concentrated, dried, you can the protein in cottonseed is obtained.
2. one kind method for extracting proteins from Cottonseed Meal according to claim 1, it is characterised in that the step b
Protease is papain or cathepsin.
3. one kind method for extracting proteins from Cottonseed Meal according to claim 1, it is characterised in that the step b
The reagent of alkali soluble addition is buck.
4. one kind method for extracting proteins from Cottonseed Meal according to claim 1, it is characterised in that the step b
Solution ph is 7.5.
5. one kind method for extracting proteins from Cottonseed Meal according to claim 1, it is characterised in that the step b
Heat time is 5h.
6. one kind method for extracting proteins from Cottonseed Meal according to claim 1, it is characterised in that the step e
Using vacuum concentration method.
7. one kind method for extracting proteins from Cottonseed Meal according to claim 1, it is characterised in that the step e
Solution water content is concentrated into 40-50%.
8. one kind method for extracting proteins from Cottonseed Meal according to claim 1, it is characterised in that the step e
Using the method for spray drying.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710180188.6A CN107058435A (en) | 2017-03-24 | 2017-03-24 | One kind method for extracting proteins from Cottonseed Meal |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710180188.6A CN107058435A (en) | 2017-03-24 | 2017-03-24 | One kind method for extracting proteins from Cottonseed Meal |
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| Publication Number | Publication Date |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206290A (en) * | 2011-04-06 | 2011-10-05 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting cottonseed oligosaccharide and cottonseed protein oligopeptide from cottonseed meal |
| CN102994605A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation |
| CN106306329A (en) * | 2015-06-25 | 2017-01-11 | 南通海辰蛋白科技有限公司 | Extraction technology of cottonseed proteins |
-
2017
- 2017-03-24 CN CN201710180188.6A patent/CN107058435A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206290A (en) * | 2011-04-06 | 2011-10-05 | 武汉普赛特膜技术循环利用有限公司 | Method for extracting cottonseed oligosaccharide and cottonseed protein oligopeptide from cottonseed meal |
| CN102994605A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Method for producing high-efficiency biological peptide through enzymolysis and microbe fermentation |
| CN106306329A (en) * | 2015-06-25 | 2017-01-11 | 南通海辰蛋白科技有限公司 | Extraction technology of cottonseed proteins |
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Application publication date: 20170818 |