CN107057643A - A kind of Fugitive dust inhibiting agent and application method based on Bacillus pasteurii culture - Google Patents
A kind of Fugitive dust inhibiting agent and application method based on Bacillus pasteurii culture Download PDFInfo
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- CN107057643A CN107057643A CN201710165461.8A CN201710165461A CN107057643A CN 107057643 A CN107057643 A CN 107057643A CN 201710165461 A CN201710165461 A CN 201710165461A CN 107057643 A CN107057643 A CN 107057643A
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Abstract
The invention discloses a kind of Fugitive dust inhibiting agent based on Bacillus pasteurii culture and application method, include the raw material of following percentage by weight：(bacterial concentration is 1.0 × 10 to 9.00%~11.00% Bacillus pasteurii bacterium solution8~1.3 × 108Cell/mL), 4.20%~4.40% CO (NH2)2, 7.90%~8.05% CaCl2With the water of surplus.The application method of the dust suppressant is：First by CaCl2With CO (NH2)2Water is dissolved according to above-mentioned percentage by weight, the Bacillus pasteurii bacterial solution of correspondence percentage is added, road surface, stockpile surface is sprayed at after being quickly well mixed；Handle 1m2Town road, required consumption is 1~3L；Handle 1m2Stockpile, required consumption is 3~6L, and it is 1~3 time to need to handle sprinkling number of times according to effect.The present invention effectively increases airborne dust inhibition using bonding, covering, biodegradation and the principle of biological calcification, has the advantages that fast reaction speed, acting duration length, freeze proof heatproof, reduction reentrainment of dust, environmental protection.
Description
Art
The present invention relates to a kind of Fugitive dust inhibiting agent technology, belong to environmental protection and Construction Technology field.
Background technology
Engineering construction scene is difficult to avoid that generation surface dust and dust, and due to factors such as wind-force, vehicle pass-throughs, periphery airborne dust is dirty
Dye is more serious, and harm resident and workman are healthy, influences construction area periphery animal and plant growth, destroys the ecological balance, causes
Extremely bad social influence, great economic loss is caused to country and enterprise.By the environment impact assessment statement for consulting all kinds of road constructions
Learn that the dust pollution of road construction site is more serious with environmentally friendly final acceptance of construction report, wherein concrete stirring, grading
(english abbreviation TSP, also known as total suspended particles refer to and use standard mass particle collection overall suspended pellet outside place at 100m
The gross mass for the particulate matter that device is collected on filter membrane) content exceeds well over ambient air quality secondary standard.
China is that State of Air pollution, than one of more serious country, counts aobvious according to State Environmental Protection Administration in the world
Show, the major pollutants in China's urban air have also been gradually converted into suspended particulate substance, suspension from sulfur dioxide, flue dust
Grain thing has become the principal element of restriction China urban air-quality, is the emphasis that urban atmospheric pollution is administered.
In the TSP of air, the contribution rate of airborne dust has met or exceeded 50%, and airborne dust particle size is smaller, easily rises
It is dynamic to fly upward, once it can be again introduced into air by external force effect, it is extremely difficult to administer.Airborne dust component is mainly soil dirt, the i.e. earth's crust
The elements such as middle silicon, calcium, aluminium mainly constitute for it.Medical discovery, long-term suction high concentration SiO2Grit, the incidence of disease of silicosis is bright
Aobvious increase.The larger particulate matter of particle diameter is largely blocked in the upper respiratory tract in airborne dust, and particle diameter is less than 10 μm of particle then
The particle of lower respiratory tract, especially particle diameter less than 2.5 μm can be entered through throat can more be deposited in alveolar.In airborne dust
PM10, PM2.5 particle are small, and specific surface area is big, because by various pollutions, be more easy to richness accumulate a large amount of harmful element such as Hg, Cr, Pb, Cu,
As etc., and its easy prolonged stay in an atmosphere, it is huge on air quality influence and human health damage, can cause for a long time it is chronic in
Poison, produces fiber lung even malignant tumour.In addition, the tiny particles content in air can not only reduce urban atmosphere visibility,
The environmental problems such as photochemical fog, acid rain, climate warming can also be caused.PM2.5 is exactly to cause the chief culprit of city On The Deterioration of Visibility Over,
Hidden trouble of traffic is added, easily causes traffic accident.
At present in city and engineering construction site road mainly using the traditional approach of watering dust suppression, conventional watering method pair
The capture rate of 2 μm of airborne dusts is only 1%~28% or so, and moisture is the surface for soaking airborne dust, it is impossible to penetrates into inside airborne dust, leads
Cause moisture evaporation fast, watering frequency is high, cause the waste and the increase of artificial and cost of equipment of great lot of water resources.Therefore both at home and abroad
Some scholars all constantly develop all kinds of dust suppressants to control dust on the roads to pollute.
Research in terms of domestic and foreign scholars have carried out dust suppressant in recent years, and applied for a collection of patent, mainly there is wetting
Type dust suppressant, cohesive dust depressor, coagulation type dust suppressant, compound dust suppressant.But all there is certain defect in all kinds of dust suppressants
And deficiency, moist type, cohesive dust depressor anti-dust effect are limited, and there is influence to the use function of road, with macromolecule
Organic matter is the coagulation type dust suppressant of representative, due to lacking certain caking property, is easily taken away by vehicles attachment, and is influenceed
Using effect.The technology of preparing of compound dust suppressant is still immature, and research and development difficulty is larger, and experiment easily fails.
At the same time, existing moist type dust suppressant, cohesive dust depressor, coagulation type dust suppressant, compound dust suppressant, by
In majority can not natural degradation in the environment, so easily causing the deterioration and pollution of environment.
The content of the invention
Technical problem：It is an object of the invention to provide a kind of reaction speed is fast, acting duration length, freeze proof heatproof, subtract
The environmentally friendly Fugitive dust inhibiting agent and application method based on Bacillus pasteurii culture of few reentrainment of dust.
Technical scheme：A kind of Fugitive dust inhibiting agent based on Bacillus pasteurii culture of the present invention includes following weight percent
The raw material of ratio：
The bacterial concentration of described Bacillus pasteurii solution is 1.0 × 108~1.3 × 108Cell/mL, i.e. bacteria liquid sample
Absorbance OD at ultraviolet specrophotometer 600nm wavelength600In the range of 1.400~1.600.
The application method of the Fugitive dust inhibiting agent based on Bacillus pasteurii culture of the present invention is：First by CaCl2And CO
(NH2)2Water is dissolved according to above-mentioned percentage by weight, the Bacillus pasteurii bacterial solution of correspondence percentage is added, it is quick mixed
Road surface, stockpile surface are sprayed at after closing uniformly；The application method is processing 1m2Town road, required consumption is 1~3L；
Handle 1m2Stockpile, required consumption is 3~6L, and it is 1~3 time to need to handle sprinkling number of times according to effect.
The present invention effectively increases airborne dust inhibition using bonding, covering, biodegradation and the principle of biological calcification,
There is fast reaction speed, acting duration length, freeze proof heatproof, reentrainment of dust, environmental protection is reduced.
Beneficial effect：The present invention compared with prior art, with advantages below：
Chemical dust suppressant can be generally divided into moist type, binding type, coagulation type and compound 4 major class.Moist type chemistry suppression
The term of validity of dirt agent is shorter, generally 5~10 days, if not repeating to spray, it may appear that reentrainment of dust；Binding type chemistry dust suppression
Most surfactants in agent component are difficult to natural degradation, and solid halide is easily washed away by rainwater, there is secondary pollution, can also
Corroding metal parts and tire, there is certain pollution to environment, and emulsifiability is poor；Hygroscopicity in coagulation type dust suppressant without
The corrosivity of machine salt is stronger, can damage metal parts and automobile tire, not only has damaging effect to the plant and soil in roadside,
Can also there are certain modification or even harm to materials such as cement, lime, high power water-absorbing resin flocculating agent preparation cost is higher, prepare
Difficulty greatly, limits it and widely used；Compound dust suppressant is more to be used under environment severe, complicated and changeable, and it is studied
Step is later, because the coupling technology of preparing mechanism such as emulsification, solubilising, coordination, wetting, ion chelating, graft copolymerization is still immature, hair
The exhibition time is shorter, causes research and development difficulty larger, tests and easily fail.
At present, the suppression of field is stacked as dirt road and granular media using crude oil, pitch, residual oil, halide, waterglass, sulfonate
The dust suppression technology of dirt agent base-material has been widely used.Although these dust suppressant anti-dust effects are good, many of which chemistry
Material can cause secondary pollution, such as solid halide, (NH to environment4)2SO4, lignin derivative etc. there is secondary pollution, again
Easily washed away by rainwater, if can also corrode tire and metallic element for road surface, the material such as many surfactants in its component
It is difficult to decompose again in its natural state to remove.If using crude oil, cinder oil, oily waste residue, the biological dregs of fat, pitch etc., its wetting is oozed
Saturating power is not strong and has certain influence on surrounding environment yet.So, existing chemical dust suppressant all has various shortcomings,
Needed in performance perfect.
CO (NH in the Fugitive dust inhibiting agent of the present invention2)2NH can be resolved into by bioactive enzyme3And CO2, CO2Can be with Ca2 +Reaction generation CaCO3；CaCO produced by reaction3With strong adhesive property, periphery airborne dust particle can be bonded to one
Rise and form bulky grain, effectively prevent rising for airborne dust, and significantly improve the anti-spreading of ground (soil property or cementaceous) dust；
The CaCO of generation3One layer of duricrust can be formed, is covered on dust, wind resistance and the anti-ability that drenches with rain is significantly improved.Bar
CaCO of the family name bacillus under 30 DEG C of hot environment and 5 DEG C of low temperature environment3Growing amount is close, illustrates that anti-dust effect is bullied
The influence very little of temperature；Under 50 DEG C of hyperthermal environments, the CaCO of generation3It is twice under normal temperature, anti-dust effect is more preferably.Raw material
In Bacillus pasteurii sprinkling after be fixed in airborne dust particle, will not permeate and be lost in, can voluntarily be degraded after a period of time, nothing
In secondary pollution, and raw material and be not added with it is any pollution environment crosslinking agent, reinforcing agent, dispersant, dropped by bioactive enzyme
CO (the NH of solution2)2Gas has been converted to it so that dust suppressant does not result in the pollution to environment.Therefore the dust suppression of the application present invention
Agent is more beneficial for environmental protection.Bacillus pasteurii can constantly expand culture, can reduce the cost of material of practical application.
The present invention sprays after only need to successively being mixed according to order of addition of ingredients with water, simple to operate without special aid, real
It is strong with property, available for dust suppressions such as urban road, building yard, Square Construction and stock grounds, do not limited by place and place.
Brief description of the drawings
Fig. 1 is dust suppressant seepage velocity measurement result；
Fig. 2 is dust suppressant wind resistance measurement result；
Fig. 3 is dust suppressant dust suppression efficiency test result；
Fig. 4 is the use schematic flow sheet of dust suppressant of the present invention.
Embodiment
Bacillus pasteurii can be purchased from China General Microbiological DSMZ, and raw material is easy to get inexpensively, due to purchase
Strain is preserved in peace with the vacuum drying of the state of freeze-dried powder falls in bottle and is cultivated, it is necessary to carry out bacterium activation with breeding, just can be used for
Dust controlling.
Bacillus pasteurii is activated：Prepare some solid mediums (solid culture based component：15g/L casein peptones,
5g/L soy peptones, 5g/L NaCl, 20g/L urea, 20g/L agar, pH=7.3), through 121 DEG C, high temperature under the conditions of 20min
After steam sterilizing, aseptic operating platform cooling is put into stand-by.With alcolhol burner heating peace neck bottle top, then drip several drip and be allowed to brokenly
Split, draw 0.4mL sterilized waters with sterilizing liquid-transfering gun and inject in inner tube, dissolve freeze-dried powder.Then sterilizing liquid-transfering gun is used from inner tube
In averagely draw 0.2mL to two solid medium, inclined-plane is at 30 DEG C after static gas wave refrigerator 72h, it can be seen that have substantially it is milky white
Color bacterium colony is generated, and Bacillus pasteurii is activated successfully.
Bacillus pasteurii culture：Prepare some fluid nutrient mediums (Liquid Culture based component：15g/L casein peptones,
5g/L soy peptones, 5g/L NaCl, 20g/L urea, pH=7.3), the inclined-plane solid culture basal orientation with bacterium colony is equipped with
The triangular flask inoculation of fluid nutrient medium.Triangular flask is put into 30 DEG C, 200rpm incubators after culture 48h, is taken out and is found nutrient solution
It is substantially muddy, it is put into centrifuge and 20min is centrifuged with 4000g rotating speed, remove after supernatant, supplements new fluid nutrient medium
Afterwards, shake up, then repeat above-mentioned steps one time；The fluid nutrient medium containing Bacillus pasteurii after cultivating successfully can be with
It is inoculated with, repeats the above steps to fluid nutrient medium, expands culture.Finally, by adding appropriate amount of fluid culture medium, by bacteria liquid sample
It is put into ultraviolet specrophotometer, makes its absorbance OD at 600nm wavelength600In the range of 1.400~1.600, i.e.,
Bacterial concentration is 1.0 × 108~1.3 × 108Cell/mL, with CaCl2、CO(NH2)2Road or stockpile spray are can be used to after mixing
Spill.
The application method of dust suppressant of the present invention is：By CaCl2、CO(NH2)2Water is dissolved according to above-mentioned percentage by weight, then
Corresponding Bacillus pasteurii bacterium solution is added, road surface, stockpile surface are sprayed at after Quick uniform mixing；Handle 1m2Municipal road
Road, required consumption is 1~3L；Handle 1m2Stockpile, required consumption is 3~6L, needs processing to spray number of times according to effect and is
1~3 time.
With reference to embodiment and Figure of description, the present invention is further illustrated.
Embodiment 1：
The first step：By CO (NH2)2With CaCl2It is well mixed in input ratio water.
Second step：Add Bacillus pasteurii bacterial solution.
3rd step：Road surface, stockpile surface are sprayed at after being quickly well mixed.
CO (the NH of said ratio2)2With CaCl2About 123kg, is added to 0.9m3Water in, after stirring evenly, add about 0.1m3
Bacillus pasteurii solution (bacterial concentration be 1.0 × 108~1.3 × 108Cell/mL), to 2000m after quick mixing2Left and right
Urban pavement sprayed, result of implementation shows, weight proportion for 9.00% Bacillus pasteurii solution, 4.20% CO
(NH2)2, 7.90% CaCl2When, the TSP after sprinkling in 7 days is 0.30mg/m3, in ambient air quality grade III Standard
Within；Bacillus pasteurii solution, 4.40% CO (NH of the weight proportion for 11.00%2)2, 8.05% CaCl2When, sprinkling
The TSP in 7 days is 0.27mg/m afterwards3, within ambient air quality grade III Standard；Weight proportion is 10.00% bar
Family name bacillus solution, 4.32% CO (NH2)2, 7.99% CaCl2When, the TSP after sprinkling in 7 days is 0.26mg/m3,
Within ambient air quality grade III Standard.
In order to contrast the effect of dust suppressant common on the market and Bacillus pasteurii dust suppressant, pure water, CaCl are chosen2、
These three typical dust suppressants of hydroscopic high-molecular resin (SAP) are reference, are entered with the application performance of Bacillus pasteurii dust suppressant
Row contrast test.Wherein, CaCl2Dust suppression agent concentration is that 0.24%, SAP dust suppressants are 0.2%.Experiment includes the infiltration of dust suppressant
Speed, wind resistance, dust suppression efficiency.
Embodiment 2：The seepage velocity of dust suppressant
A certain amount of coal dust is fitted into the test tube that φ × h is 20 × 200mm first, glass bar compacting coal dust makes test tube
The height of middle coaling powder is 15cm, is then fixed on rack for test tube；2mL dust suppression agent solution is finally slowly added into, is delayed
It is slow to instill in the glass tube equipped with coal dust, the depth that recording solution permeates in 20 minutes.Under the same conditions, solution
Seepage velocity it is faster, illustrate that the permeability of solution is better.
It will be seen from figure 1 that the penetration depth of SAP and Bacillus pasteurii dust suppressant in a short time is maximum, coal is soaked
The speed of powder, illustrates that its wettability is stronger, and after dust suppression agent solution is spread on coal dust, certain depth can be soaked quickly
Coal dust, faster play dust suppression effect.
Embodiment 3：The wind resistance of dust suppressant
The quality of φ 90mm culture dishes is first weighed, 15mL dust suppressions agent solution is uniformly drenched on culture dish, its weight is determined；
Wind resistance experiment is carried out to dust suppressant (to enter dust suppressant with mechanical 3 grades of the stand fans of FS40-B3B type Midea 400mm
Row blowing, the rotating shaft of flabellum is just to culture dish, and dry 8h at the 1m of culture dish side), culture dish quality is determined, and according to following
Formula obtains wind resistance index.Wind resistance index reflect dust suppressant under conditions of fresh breeze own wt lose number, wind resistance
Index is bigger, illustrates that dust suppressant keeps the ability of moisture stronger under high wind conditions, cured layer is also not easy to be blown up, effectively
The time of dust suppression is longer.The calculation formula of wind resistance index is：
In formula, M --- wind resistance index；
m1--- the quality of culture dish, g；
m2--- culture dish and dust suppressant quality, g；
m3--- wind resistance tests the quality for terminating rear culture dish and dust suppressant, g.
Figure it is seen that the wind resistance index of dust suppressant is followed successively by from high to low：Bacillus pasteurii ＞ SAP ＞ CaCl2
＞ pure water.Pure water and CaCl2Hardly possess wind loading rating, not only moisture evaporation is fast under storm wind weather, and dust holds very much
It can easily be blown afloat, atmospheric environment polluted again；And SAP and urase dust suppressant wind loading rating are very strong, high wind can be effective against
Influence；Wind loading rating is most strong for urase dust suppressant, under storm wind weather, remains to keep stronger anti-evaporating performance.
Embodiment 4：The dust suppression efficiency of dust suppressant
The quality of φ 90mm culture dishes is first weighed, 20g coal dusts are loaded in culture dish；By 15mL dust suppressions agent solution equably
Drench on coal dust surface, and determine its quality；Wind resistance experiment is carried out to the coal dust for having covered dust suppressant and (uses FS40-B3B types
Coal dust surface is blowed Midea 400mm 3 grades of stand fans of machinery, and dry 8h at the 1m of coal dust side)；Wind resistance experiment knot
Shu Hou, is weighed to the coal dust of the upper dust suppressant of covering with culture dish.Dust suppression efficiency reflects condition of the dust suppressant in fresh breeze
The lower ability for suppressing airborne dust, wind resistance efficiency is higher, illustrates that the airborne dust blown afloat is fewer, anti-dust effect is better.Dust suppression efficiency calculation
Formula：
In formula, η --- dust suppression efficiency, %；
m1--- the quality of culture dish, g；
n1--- the quality of coal dust and culture dish, g after addition dust suppressant；
n2--- wind resistance experiment terminates the quality of rear dust suppressant covering coal dust and culture dish, g.
From figure 3, it can be seen that the dust suppression efficiency of 4 kinds of dust suppressants is followed successively by from high to low：Bacillus pasteurii ＞ SAP ＞
CaCl2＞ pure water.Wherein, under the dust suppression efficiency highest of Bacillus pasteurii dust suppressant, prolonged wind load test, dust suppression effect
Rate can be up to 80%；Secondly, dust suppression efficiency is also higher, can reach 69% by SAP；Pure water, calcium chloride dust suppression efficiency compared with
It is low, only 60%.
Above-described embodiment is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill of the art
For personnel, under the premise without departing from the principles of the invention, some improvement and equivalent substitution can also be made, these are to the present invention
Claim be improved with the technical scheme after equivalent substitution, each fall within protection scope of the present invention.
Claims (3)
1. a kind of Fugitive dust inhibiting agent based on Bacillus pasteurii culture, it is characterised in that the Fugitive dust inhibiting agent includes following heavy
Measure the raw material of percentage：
2. a kind of Fugitive dust inhibiting agent based on Bacillus pasteurii culture according to claim 1, it is characterised in that described
Bacillus pasteurii solution bacterial concentration be 1.0 × 108~1.3 × 108Cell/mL, i.e. bacteria liquid sample are in ultraviolet spectrometry light
Absorbance OD at degree meter 600nm wavelength600In the range of 1.400~1.600.
3. a kind of application method of the Fugitive dust inhibiting agent as claimed in claim 1 based on Bacillus pasteurii culture, its feature
It is, first by CaCl2With CO (NH2)2Water is dissolved according to above-mentioned percentage by weight, Pasteur's gemma of correspondence percentage is added
Bacillus bacterial solution, road surface, stockpile surface are sprayed at after being quickly well mixed；The application method is processing 1m2Town road,
Required consumption is 1~3L；Handle 1m2Stockpile, required consumption is 3~6L, according to effect need to handle sprinkling number of times be 1~
3 times.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603637A (en) * | 2017-10-19 | 2018-01-19 | 朱东洋 | A kind of preparation method of biological soil curing agent |
| CN108214799A (en) * | 2017-12-18 | 2018-06-29 | 雷笑天 | A kind of preparation method of insect prevention flame-retardant solid wood flooring slab |
| CN109706094A (en) * | 2018-12-29 | 2019-05-03 | 中国水利水电科学研究院 | A kind of dust suppressant and preparation method thereof and application method |
| CN110305629A (en) * | 2019-07-02 | 2019-10-08 | 山东科技大学 | A kind of the coal mine compound dust suppressant of surfactant-microorganism and its application method |
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|---|---|---|---|---|
| CN103820123A (en) * | 2014-01-28 | 2014-05-28 | 东南大学 | Microbial sand-fixing dust depressor and preparation method thereof |
| WO2016145190A1 (en) * | 2015-03-10 | 2016-09-15 | Biomason, Inc. | Compositions and methods for dust control and the manufacture of construction materials |
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2017
- 2017-03-20 CN CN201710165461.8A patent/CN107057643A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820123A (en) * | 2014-01-28 | 2014-05-28 | 东南大学 | Microbial sand-fixing dust depressor and preparation method thereof |
| WO2016145190A1 (en) * | 2015-03-10 | 2016-09-15 | Biomason, Inc. | Compositions and methods for dust control and the manufacture of construction materials |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603637A (en) * | 2017-10-19 | 2018-01-19 | 朱东洋 | A kind of preparation method of biological soil curing agent |
| CN108214799A (en) * | 2017-12-18 | 2018-06-29 | 雷笑天 | A kind of preparation method of insect prevention flame-retardant solid wood flooring slab |
| CN109706094A (en) * | 2018-12-29 | 2019-05-03 | 中国水利水电科学研究院 | A kind of dust suppressant and preparation method thereof and application method |
| CN110305629A (en) * | 2019-07-02 | 2019-10-08 | 山东科技大学 | A kind of the coal mine compound dust suppressant of surfactant-microorganism and its application method |
| CN110305629B (en) * | 2019-07-02 | 2022-01-28 | 山东科技大学 | Surfactant-microorganism composite dust suppressant for coal mine and using method thereof |
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Application publication date: 20170818 |