CN107047922A - The preparation method and chick-pea egg mix white powder of chick-pea egg mix white powder - Google Patents
The preparation method and chick-pea egg mix white powder of chick-pea egg mix white powder Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a kind of preparation method of chick-pea egg mix white powder.Mainly include the following steps that：1) chick-pea, mulberry leaf powder and buckwheat powder are mixed and soaked, alkalescence processing is beaten to obtain leachate；2) leachate is heated, cooled down, centrifuged, obtain supernatant；3) weak acid precipitating proteins are used, high speed centrifugation retains precipitation, finally freeze-drying obtains chick-pea egg mix white powder.This preparation method adds mulberry leaf powder and buckwheat powder first, can further lift the nutritional ingredient and healthcare function of the albumen powder of preparation；And in protein extracting method, by the way of a small amount of addition, for a long time immersion, alkalescence processing, farthest retain nutritional ingredient；Using weak acid precipitating proteins, safety and can be with the nutrition composition of retaining protein.The anti-aging effects of the egg mix white powder of the present invention are very good, and add after mulberry leaf powder and buckwheat powder, and the anti-aging effects of albumen powder are significantly increased.
Description
Technical field
The present invention relates to biological technical field, especially, it is related to preparation method and the hawk of a kind of chick-pea egg mix white powder
Garbanzo egg mix white powder.
Background technology
Chick-pea (Cicer arietinum) belongs to vetch race, because of its unique outlook, similar olecranon and is referred to as olecranon
Beans, are current Food Legume crops second largest in the world.18 kind amino acid of the chick-pea rich in needed by human body are (wherein needed by human
8 kinds of amino acid content and human body requirements ratio it is very much like) and protein, dietary fiber, trace element and vitamin etc.
Nutriment.Chick-pea is equal to diabetes, high fat of blood, hypertension, cardiovascular and cerebrovascular disease, tuberculosis, indigestion, disease of skin etc.
Have good herbal cuisine and dietary function, available for daily bread and nutrition fortifier, to enhancing body immunity, anti-aging,
Beautifying face and moistering lotion and children's intelligence development are all of great advantage.
Chickpea Protein is the quality plant protein of a kind of hypoallergenic, high biological value.Chickpea Protein content because
Kind difference and have larger difference, between 15-30%.Traditional Chickpea Protein for preparing all is that alkali extraction and acid precipitation obtains egg
White sediment, can add strong acid, highly basic can destroy the part nutritive value of albumen powder, and leave unsafe factor.Traditional system
Standby Chickpea Protein is all that other auxiliary materials are only not added with chick-pea, and nutritional ingredient is relatively single.
In order to solve the above technical problems, this patent provides the strengthen immunity chick-pea for being adapted to large-scale industrial production
The preparation technology of egg mix white powder, and mulberry leaf powder and buckwheat powder are added during Chickpea Protein is prepared first so that egg
White matter quality is further lifted.
The content of the invention
Present invention aims at a kind of preparation method of chick-pea egg mix white powder is provided, to solve technical problem.
A kind of preparation method of chick-pea egg mix white powder, comprises the following steps：
A, chick-pea, mulberry leaf powder and buckwheat powder soaked, add buck, adjust pH, mixture is broken into slurry, filtering
Obtain bean dregs and leachate；Bean dregs are handled twice again by above-mentioned steps.
B, the leachate heated in a water bath, be subsequently cooled to after room temperature, centrifuge, take supernatant；
C, add weak acid and do not stop stirring, keeping temperature certain temperature, precipitating proteins；
D, high speed centrifugation, filter and reclaim sediment fraction；
E, precipitation progress is freeze-dried and obtains strengthen immunity chick-pea egg mix white powder.
3rd, the preparation method of chick-pea egg mix white powder described above, it is characterised in that in described step A, chick-pea,
Mulberry leaf powder and buckwheat powder soak time are 18-24 hours；The addition of mulberry leaf powder and buckwheat powder is respectively the 3-5% of dry beans quality
And 1-3%；Tune solution ph is 7-9.
3rd, the preparation method of chick-pea egg mix white powder described above, it is characterised in that in described step B, the water
Heating-up temperature is bathed in 50-60 DEG C, time 10-15 minute；The centrifugal rotational speed 4500-8000rpm, time 10-20 minute.
4th, the preparation method of chick-pea egg mix white powder described above, it is characterised in that described weak in described step C
Acid is the one or more in acetic acid, lactic acid, citric acid, adjusts pH to 3-5,40-60 DEG C of whipping temp, time 8-12h.
5th, the preparation method of chick-pea egg mix white powder described above, it is characterised in that in described step D, it is described from
Heart speed 5000-10000rpm, centrifugation time 10-20 minutes.
The preparation method of the chick-pea egg mix white powder of the present invention, adds mulberry leaf powder and buckwheat powder first, can be further
Lift the nutritional ingredient healthcare function of the albumen powder prepared.Mulberry leaf powder and buckwheat powder are as follows to the main function of body：
Mulberry leaf are the dried leafs of moraceae plants mulberry.Harvested after first frost, remove impurity, dry and obtain, be a kind of diverging wind-heat
Medicine, both oral administrations also can external application.Its is cold in nature, sweet, hardship, has functions that dispelling wind and heat from the body, clearing away the lungheat and moisturizing, clears liver and improve vision, can treat
Anemopyretic cold, lung-heat type cough, dizziness headache, the dim-sighted illness of hot eyes.In modern times, doctor trained in Western medicine using mulberry leaf and mulberry leaf biological agent as
Improve the medicine of diabetes and other various difficult and complicated cases and use, it is believed that its drug effect is extremely wide.Have clearing away the lungheat and moisturizing, cough-relieving,
Reduce phlegm and internal heat, resolving sputum, control night sweat；Tonifying liver, clear liver and improve vision, treat it is dizzy, insomnia, eliminate eye strain；Subside a swelling, purify the blood ' treatment
Dysentery, stomachache, fat-reducing, except tinea pedis, sharp large and small intestines；Resisting stress, cool blood, lowering blood pressure and blood fat, prevention miocardial infarction, brain overflow
Blood, dispel headache, long hair；Hypoglycemic, anti-sugared disease etc..
Bitter buckwheat is medicine-food two-purpose crop very few in nature, according to《Compendium of Materia Medica》Record：Bitter buckwheat bitter, cold, energy
Real stomach, benefiting energy continues spirit, and sharp knowledge, refining the five internal organs slag is dirty；《Qianjin Yao Fang》、《Dictionary of medicinal plant》And in pertinent literature
It is all on the books to bitter buckwheat：Can calm the nerves, lively atmosphere blood, the wide intestines of sending down abnormally ascending, heat-clearing swell wind pain, stagnant productization of dispelling, gut purge, ease constipation, defaecation, stop
Cough, relieving asthma, anti-inflammatory, antiallergy, cardiac stimulant, fat-reducing, beauty the effects such as.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.These embodiments are interpreted as being merely to illustrate this hair
It is bright rather than limit the scope of the invention.After the content of the invention recorded has been read, those skilled in the art can
To be made various changes or modifications to the present invention, these equivalence changes and modification equally fall into the model that the claims in the present invention are limited
Enclose.In the present invention, refered in particular to as non-, all amounts, part, percentage is weight, and all raw material can be purchased from market
.
Embodiment 1
A kind of preparation method of chick-pea egg mix white powder, comprises the following steps：
A, by chick-pea, mulberry leaf powder and buckwheat powder soak 22 hours after, add buck, regulation pH be 8.1, mixture is beaten
Into slurry, filtering removes bean dregs, obtains leachate；Bean dregs are handled twice again by above-mentioned steps.
B, the leachate heated 12 minutes in 55 DEG C of tepidarium, is cooled to after room temperature, with 6500rpm speed from
The heart 15 minutes, supernatant is used to extract albumen powder；
C, addition lemon acid for adjusting pH are that 4.2 water-bath, 50 DEG C of stirring 10h are precipitated；
D, centrifugation is carried out under 8000rpm speed, filter and reclaim sediment fraction；
E, precipitation progress is freeze-dried and obtains Chickpea Protein powder；
In described step A, described mulberry leaf powder and the addition of buckwheat powder are respectively the 4% and 2.5% of dry beans quality.
Embodiment 2
A kind of preparation method of chick-pea egg mix white powder, comprises the following steps：
A, by chick-pea, mulberry leaf powder and buckwheat powder soak 18 hours after, add buck, regulation pH be 8.0, mixture is beaten
Into slurry, filtering removes bean dregs, obtains leachate；Bean dregs are handled twice again by above-mentioned steps.
B, the leachate heated 10 minutes in 60 DEG C of tepidarium, is cooled to after room temperature, with 5500rpm speed from
The heart 10 minutes, supernatant is used to extract albumen powder；
C, the newborn acid for adjusting pH of addition are that 4.3 water-bath, 55 DEG C of stirring 12h are precipitated；
D, centrifugation is carried out under 8500rpm speed, filter and reclaim sediment fraction；
E, precipitation progress is freeze-dried and obtains Chickpea Protein powder.
In described step A, described mulberry leaf powder and the addition of buckwheat powder are respectively the 3% and 3% of dry beans quality.
Embodiment 3
A kind of preparation method of chick-pea egg mix white powder, comprises the following steps：
A, by chick-pea, mulberry leaf powder and buckwheat powder soak 24 hours after, add buck, regulation pH be 7..Mixture is beaten
Into slurry, filtering removes bean dregs, obtains leachate；Bean dregs are handled twice again by above-mentioned steps.
B, the leachate heated 15 minutes in 50 DEG C of tepidarium, is cooled to after room temperature, with 4500rpm speed from
The heart 20 minutes, supernatant is used to extract albumen powder；
C, addition vinegar acid for adjusting pH are that 4.3 water-bath, 55 DEG C of stirring 12h are precipitated；
D, centrifugation is carried out under 7500rpm speed, filter and reclaim sediment fraction；
E, precipitation progress is freeze-dried and obtains Chickpea Protein powder.
In described step A, described mulberry leaf powder and the addition of buckwheat powder are respectively the 5% and 1% of dry beans quality.
Comparative example 1
Mulberry leaf powder in embodiment 1 and buckwheat powder are removed, other preparation conditions are constant.
Mouse stomach test is carried out to embodiment 1-3 and the albumen powder of comparative example 1 below, specific method of testing is such as
Under：
50 mouse are randomly divided into 5 groups by body weight：Blank control group, embodiment 1-3 and comparative example 1, every group 10
Only, the standard feed sub-cage rearing provided with Beijing Vital River Experimental Animals Technology Co., Ltd., free diet drinking-water.Adapt to
Property feed 5 days after carry out gavage experiment.
Give experimental mice gavage respectively daily, mouse timing determines body weight, the amount of gavage, gavage amount are calculated according to body weight
For：1.7g/ days/kg, continuous gavage 30 days.Test cell immune function, humoral immune function index and NK cytoactives.
Table 1：Blank control group, embodiment, comparative example mouse cell immunologic function, humoral immune function and NK cells
Activity.
Different letters represent significant difference (p to data (mean+SD) afterwards in same column<0.05, n=3)
As a result show, less, animal activity in process of the test is normal, table without exception for the development difference of each group mouse weight
It is existing, show albumen powder to mouse grow and health has no adverse effects.Specific mouse cell immunologic function, humoral immunity work(
Can be with NK cytoactives result such as table 1.By the test data of table 1 it is recognised that the strengthen immunity of the albumen powder by the present invention
Effect is very good, and adds after mulberry leaf powder and buckwheat powder, and the strengthen immunity effect of albumen powder is significantly increased.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.
Claims (6)
1. a kind of preparation method of chick-pea egg mix white powder, it is characterised in that comprise the following steps：
A, chick-pea, mulberry leaf powder and buckwheat powder soaked, add buck, adjust pH, mixture is broken into slurry, beans are filtered to obtain
Slag and leachate；Bean dregs are handled twice again by above-mentioned steps；
B, the leachate heated in a water bath, be subsequently cooled to after room temperature, centrifuge, take supernatant；
C, add weak acid and do not stop stirring, keep certain temperature, precipitating proteins；
D, high speed centrifugation, reclaim precipitation；
E, the precipitation progress is freeze-dried and obtains chick-pea egg mix white powder.
2. the preparation method of chick-pea egg mix white powder as claimed in claim 1, it is characterised in that in described step A, institute
Chick-pea, the mulberry leaf powder and the buckwheat powder soak time are stated for 18-24 hours；The addition difference of mulberry leaf powder and buckwheat powder
For the 3-5% and 1-3% of the chick-pea quality；Alkali is sodium hydroxide or calcium hydroxide, and regulation pH value is 7-9.
3. the preparation method of chick-pea egg mix white powder as claimed in claim 1, it is characterised in that in described step B, institute
Water bath heating temperature is stated in 50-60 DEG C, time 10-15 minute；The centrifugal rotational speed 4500-8000rpm, time 10-20 minute.
4. the preparation method of chick-pea egg mix white powder as claimed in claim 1, it is characterised in that in described step C, institute
It is the one or more in acetic acid, lactic acid, citric acid to state weak acid, adjusts pH to 3-5；40-60 DEG C of whipping temp, time 8-12h.
5. the preparation method of chick-pea egg mix white powder as claimed in claim 1, it is characterised in that in described step D, institute
State centrifugal speed 5000-10000rpm, centrifugation time 10-20 minutes.
6. a kind of chick-pea egg mix white powder, it is characterised in that according to the preparation method any one of claim 1 to 5
It is made.
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| CN (1) | CN107047922A (en) |
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| CN107712263A (en) * | 2017-11-22 | 2018-02-23 | 南京财经大学 | A kind of method of synchronous extraction rice and golden mushroom residue protein |
| CN107805653A (en) * | 2017-11-02 | 2018-03-16 | 林峰 | Chick-pea active peptide, composition and preparation method |
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| CN101045088A (en) * | 2006-03-28 | 2007-10-03 | 山西亚宝药业集团股份有限公司 | Medicine composition for treating diabetes and its preparing method |
| CN102504009A (en) * | 2011-11-17 | 2012-06-20 | 中国科学院西北高原生物研究所 | Production method of active boxthorn leaf albumen powder |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107805653A (en) * | 2017-11-02 | 2018-03-16 | 林峰 | Chick-pea active peptide, composition and preparation method |
| CN107712263A (en) * | 2017-11-22 | 2018-02-23 | 南京财经大学 | A kind of method of synchronous extraction rice and golden mushroom residue protein |
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