CN107047453B - Wild fence breeding method for rodents - Google Patents
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- CN107047453B CN107047453B CN201710019866.0A CN201710019866A CN107047453B CN 107047453 B CN107047453 B CN 107047453B CN 201710019866 A CN201710019866 A CN 201710019866A CN 107047453 B CN107047453 B CN 107047453B
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- 241000283984 Rodentia Species 0.000 title claims abstract description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 208
- 229910052742 iron Inorganic materials 0.000 claims abstract description 104
- 241001465754 Metazoa Species 0.000 claims abstract description 43
- 241000283973 Oryctolagus cuniculus Species 0.000 claims abstract description 22
- 230000000694 effects Effects 0.000 claims abstract description 19
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 229910001220 stainless steel Inorganic materials 0.000 claims description 32
- 239000010935 stainless steel Substances 0.000 claims description 32
- 239000003973 paint Substances 0.000 claims description 20
- 229910000746 Structural steel Inorganic materials 0.000 claims description 16
- 230000004083 survival effect Effects 0.000 claims description 13
- 239000002689 soil Substances 0.000 claims description 12
- 238000010171 animal model Methods 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 8
- 210000003625 skull Anatomy 0.000 claims description 7
- 238000011160 research Methods 0.000 claims description 6
- 230000007774 longterm Effects 0.000 claims description 5
- 239000004743 Polypropylene Substances 0.000 claims description 4
- 229920000180 alkyd Polymers 0.000 claims description 4
- 230000037396 body weight Effects 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000003086 colorant Substances 0.000 claims description 4
- 239000003085 diluting agent Substances 0.000 claims description 4
- 238000004043 dyeing Methods 0.000 claims description 4
- 239000002320 enamel (paints) Substances 0.000 claims description 4
- 239000003822 epoxy resin Substances 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 230000037308 hair color Effects 0.000 claims description 4
- 239000000118 hair dye Substances 0.000 claims description 4
- 235000000396 iron Nutrition 0.000 claims description 4
- 244000144972 livestock Species 0.000 claims description 4
- 229920000647 polyepoxide Polymers 0.000 claims description 4
- -1 polypropylene Polymers 0.000 claims description 4
- 229920001155 polypropylene Polymers 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 238000005553 drilling Methods 0.000 claims description 3
- 241000555745 Sciuridae Species 0.000 claims description 2
- 239000000975 dye Substances 0.000 claims description 2
- 230000003014 reinforcing effect Effects 0.000 claims description 2
- 238000003975 animal breeding Methods 0.000 abstract description 2
- 241000699670 Mus sp. Species 0.000 abstract 3
- 206010021143 Hypoxia Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000283960 Leporidae Species 0.000 description 1
- 241000880150 Ochotona curzoniae Species 0.000 description 1
- 101100534473 Rattus norvegicus Stab2 gene Proteins 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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Abstract
The invention discloses a wild fence breeding method of rodents, belonging to the technical field of animal breeding and comprising the following steps: an iron sheet fence is constructed on the natural grassland in the field, the iron sheet is buried underground for 0.5 m, the iron sheet is reserved on the ground, and an artificial tunnel with the diameter of 15 cm and the distance from the ground to the ground is excavated every 10 m by using a tunnel excavator to construct the artificial fence in the field. In months 5-10, healthy adult plateau mice and rabbits were captured and placed in pens, 30 mice per pen, and 15 mice each. Animals were kept in pens for 1-7 days, and individual activity was observed and recorded daily. The method is suitable for outdoor breeding of rodents living on the ground and excavating tunnels.
Description
Technical Field
The invention belongs to the technical field of animal breeding, and particularly relates to a field fence breeding method for rodents.
Background
The plateau rabbit Ochotona curzoniae is a non-hibernating small rodent distributed in high altitude areas, belongs to Leporidae of Lepidoptera, and is a specific species of Tibet plateau. The plateau rabbits are mainly distributed in alpine meadows and grassland areas with the elevation of 3100-. The plateau rat hare lives in a cave, has a developed underground tunnel system, but has weak individual digging capability, and mainly utilizes ground surface cracks to dig a tunnel on the basis of the ground surface cracks.
The plateau rat rabbit is a species developed along with the elevation of the Qinghai-Tibet plateau, has good adaptability to plateau low temperature, hypoxia and the like, and is an ideal experimental animal for researching alpine and hypoxia. In the technical research of a human and animal co-diseased infection model and the experimental animalization of wild animals special in China which are the key research projects of the national science and technology support of 'fifteen', the plateau rabbits are taken as key research objects. After experimental treatment of animals, many studies require long-term monitoring of their behavior, physiology, etc., and therefore, it is desirable to establish an experimental population of rodents in the wild natural state. As the digging ability of the rabbits is weak, and the alpine meadow turf is hard, the digging of the rabbits is not facilitated; the natural population is not beneficial to the development of various experimental operations, and a breeding method of the field fence population needs to be established urgently. However, no research report on breeding of the plateau rabbit under the field artificial fence exists at home and abroad so far.
Early studies on plateau rabbits focused mainly on natural grasslands or indoor conditions, both of which had significant drawbacks. In an open natural population of a natural grassland, individuals are easy to migrate, the population cannot be effectively controlled, and the repeated capture probability of target individuals is low, so that effective experimental detection is difficult to perform; under indoor conditions, animals are in a stressed state for a long time and cannot reflect the real situation in a natural state.
Disclosure of Invention
The invention aims to provide a wild fence breeding method for rodents, which is suitable for outdoor breeding of the rodents living on the ground and excavating tunnels.
The specific technical scheme is as follows:
a method for breeding rodents in a field fence comprises the following steps:
step 1, selecting a field natural grassland with flat terrain and good vegetation growth condition during 5-10 months, constructing an iron sheet fence with length multiplied by width of 60 multiplied by 80 meters, and cutting an iron sheet roll into a plate with the length of 10 meters by using a plate cutting machine; drilling holes in proper positions on the iron sheet by using a drill bit with the diameter of 4-6 mm so as to fix the iron sheet; in the vertical direction, angle iron and stainless steel screws with the width of 5 multiplied by 5 centimeters and the height of 2 meters are used for fixing iron sheets every 2 meters, 1-2 stainless steel gaskets are arranged between a screw and the iron sheets and between the screw and the nuts, stainless steel angle iron and stainless steel screws with the width of 10 multiplied by 10 centimeters and the height of 2 meters are used for fixing the iron sheets every 10 meters, and stainless steel gaskets are arranged between the screw and the iron sheets and between the screw and the nuts; in the horizontal direction, 2 iron sheets are fixed by stainless steel flat irons and stainless steel screws with the width of 8 centimeters, and stainless steel washers are arranged among the screws, the iron sheets and the nuts to enable the iron sheets to be integrated; checking the fence every 1 month, and reinforcing the loosened iron sheet;
step 2, digging a trench with the depth of 50 cm and the width of 50 cm by using a small excavator, burying the iron sheet and the angle iron together into the ground for 0.5 m, and reserving 1.5 m of the iron sheet on the ground; backfilling soil, planting seeds of the grassland native vegetation, and tamping the backfilled soil by using a tamping machine to prevent animals from digging and escaping; the depth of the underground buried iron sheet is determined by the physicochemical property of local soil, the excavating capacity of experimental animals, the depth of a cave under the natural condition and the like; digging artificial tunnels with the diameter of 15 cm and the distance of 30 cm from the ground every 10 m by using a tunnel excavator in the iron sheet fence to construct a field artificial fence; a fishing net with 10 multiplied by 10 cm of grid is used for covering the fence to prevent prey of prey birds; a net fence is used for surrounding the position 50 cm away from the periphery of the iron sheet fence to prevent and treat the interference of livestock; in order to reduce the influence of iron sheet reflection on experimenters, surrounding environment and experimental animals and prolong the service life of the iron sheet, green metallic paint or alkyd enamel paint, epoxy resin paint and the like are used, and are sprayed on the iron sheet, angle iron, flat iron and screws after being matched with a diluent and a curing agent, and paint is sprayed for at least 2-3 times; after the paint is dried, marking a number of 0-60 or 0-80 on the iron sheet at a distance of 1 meter from the ground by using red paint at each 2 meters, so as to record the coordinate position of the animal at a later stage; an observation platform with the height of 3 meters and the length and width of 1 meter is built at the position 3-5 meters outside the fence, and can reach the top of the observation platform through the steps, so that long-term observation is facilitated;
step 3, in 5-10 months, capturing healthy adult rat rabbits by using a live capture cage in the grassland with a large population quantity of the rat rabbits in the plateau, at the peak period of animal activity of 7:00-11:00 in the morning and 16:00-19:00 in the afternoon; each individual is identified with sex, weighed, measured for body length, skull length and the like, and then the individual is doubly marked by using plastic ear tags with different colors and passive electronic tags; using a human black hair dye to dye individuals, dyeing the head of a male individual and dyeing the back of a female individual so as to observe the activity condition of an animal remotely; placing the marked individuals into a polypropylene plastic squirrel cage with the length, the width and the height of 200, 350 and 475 millimeters independently for rest for 15 minutes, and slightly placing the marked individuals into a fence after the marked individuals recover to be normal; each fence is provided with 30 pens, and each pen is provided with 15 males and females; the animals are placed in the fence for 1-7 days, at the peak of animal activity of 7:00-11:00 in the morning and 16:00-19:00 in the afternoon every day, the experimenter observes and records the animal activity condition by using a high-power telescope on an observation platform outside the fence; re-capturing all individuals on day 7, checking whether ear tags and passive electronic tags are intact, judging whether the health conditions of the individuals are good or not by weighing the body weight, measuring the body length and the cranial length, observing the hair color, the activity ability and the like, and calculating the survival rate of the individuals within day 7; firstly, excavating an artificial tunnel by using the plateau rat rabbit, and excavating a complex tunnel; through observation and recapture examination for 7 days, the survival condition of the individual is good, and the survival rate within 7 days is over 95 percent, which indicates that the animal is suitable for the artificially constructed fence environment and relevant follow-up research is carried out.
Further, in the step 1, the iron sheet is made of a galvanized material, the thickness of the iron sheet is 0.8-1.22 mm, the thickness of the galvanized layer is greater than 0.02 mm, and the width of the iron sheet is 1 m.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the plateau rabbit is placed in the fence, the population is very stable, and the migration and diffusion of individuals are avoided. The total survival rate of the plateau rabbits in the fence in winter reaches 70-80 percent, is similar to natural population and is far higher than indoor population; in addition, food does not need to be added into the fence, so that the labor and financial cost is reduced; the capture probability of individuals in the fence is up to more than 90 percent and is far higher than 40 to 50 percent of the natural open population. The breeding of the plateau rat rabbit in a fence is an effective method for carrying out various experiments on wild animals. The method is suitable for outdoor breeding of rodents living on the ground and excavating tunnels.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific embodiments.
Example 1
A method for breeding rodents in a field fence comprises the following steps:
and in the period of 5 months, selecting a field natural grassland with flat terrain and good vegetation growth condition, and constructing a galvanized iron fence with the length multiplied by the width of 60 multiplied by 80 meters. The size of the enclosure is determined according to the field range, the mobility and the experimental purpose of the animal. In order to ensure the stability of the fence, the purchased galvanized iron sheet has the thickness of 0.8 mm, the thickness of the galvanized iron sheet is more than 0.02 mm, and the width of the galvanized iron sheet is 1 m. And cutting the iron sheet roll into a plate with the length of 10 meters by using a plate cutting machine. And drilling holes in proper positions on the iron sheet by using a drill bit with the diameter of 4 mm so as to fix the iron sheet. In the vertical direction, angle iron and stainless steel screws (1-2 stainless steel washers are placed between a screw and an iron sheet and between nuts) with the width of 5 multiplied by 5 cm and the height of 2 m are used for fixing the iron sheet every 2 m, and stainless steel angle iron and stainless steel screws (stainless steel washers are placed between the screw and the iron sheet and between the screw and the nuts) with the width of 10 multiplied by 10 cm and the height of 2 m are used for fixing the iron sheet every 10 m; in the horizontal direction, 2 iron sheets are fixed by stainless steel flat irons with the width of 8 cm and stainless steel screws (stainless steel washers are arranged among screw rods, the iron sheets and nuts), so that the iron sheets are integrated. The enclosure is checked every 1 month to reinforce the loosened iron sheet.
Digging a trench with the depth of 50 cm and the width of 50 cm by using a small excavator, burying the iron sheet and the angle iron together into the ground for 0.5 m, and reserving the iron sheet on the ground for 1.5 m. Backfilling soil, planting seeds of the grassland native vegetation, and tamping the backfilled soil by using a tamping machine to prevent animals from digging and escaping. The depth of the underground buried iron sheet is determined by the physicochemical properties of local soil, the excavating capacity of experimental animals, the depth of a cave under natural conditions and the like. In the iron sheet fence, an artificial tunnel with the diameter of 15 cm and the distance of 30 cm from the ground is excavated every 10 m by using a tunnel excavator, so that a field artificial fence is constructed. The top of the fence is covered by a fishing net with 10 multiplied by 10 cm of grid to prevent predation of prey birds. The periphery of the iron sheet fence is surrounded by a net fence at a position 50 cm away from the iron sheet fence, so that interference of livestock is prevented. In order to reduce the influence of the reflection of the galvanized iron sheet on experimenters, the surrounding environment and experimental animals and prolong the service life of the iron sheet, green metallic paint or alkyd enamel paint, epoxy resin paint and the like are used, and are matched with a diluent and a curing agent and then sprayed on the iron sheet, angle iron, flat iron and screws, and paint is sprayed for at least 2 times. After the paint dries, red paint is used to mark a number, 0-60, on each 2 m scale at a distance of 1 m from the ground, so as to record the coordinate position of the animal at a later stage. An observation platform with the height of 3 meters and the length and the width of 1 meter is built at the position 3 meters outside the fence, and can reach the top of the observation platform through the steps, so that long-term observation is facilitated.
In 5 months, healthy adult rat rabbits were captured by using a live trap cage in grassland with a large population, at the peak of animal activity at 7:00-11:00 am and 16:00-19:00 pm. Each individual is identified with sex, weighed, measured for body length, skull length and the like, and then the individual is doubly marked by using plastic ear tags with different colors and passive electronic tags; individuals are dyed by using a human black hair dye, male individuals are dyed with heads, and female individuals are dyed with backs, so that the movement conditions of the animals can be observed at a long distance. The marked individuals are independently placed into polypropylene plastic mouse cages of 200X 350X 475 mm (length X width X height) for rest for 15 minutes, and after the marked individuals return to normal, the marked individuals are lightly placed into a fence. Each pen contained 30 pens, 15 each male and female. The animals are placed in the fence for 1-7 days, at the peak of animal activity of 7:00-11:00 in the morning and 16:00-19:00 in the afternoon every day, the experimenter observes and records the animal activity condition by using a high-power telescope on an observation platform outside the fence; and (4) capturing all individuals again on the 7 th day, checking whether the ear tags and the passive electronic tags are intact, judging whether the health conditions of the individuals are good or not by weighing the body weight, measuring the body length and the skull length, observing the hair color, the activity ability and the like, and calculating the survival rate of the individuals within 7 days. The plateau rat rabbit firstly utilizes the excavated artificial tunnel and excavates a complex tunnel at the same time. Through observation and recapture examination for 7 days, the survival condition of the individual is good, and the survival rate within 7 days is over 95 percent, which indicates that the animal is suitable for the artificially constructed fence environment, and the related follow-up study can be carried out.
Example 2
A method for breeding rodents in a field fence comprises the following steps:
during 10 months, the field natural grassland with flat terrain and good vegetation growth condition is selected to construct the galvanized iron fence with the length multiplied by the width of 60 multiplied by 80 meters. The size of the enclosure is determined according to the field range, the mobility and the experimental purpose of the animal. In order to ensure the stability of the fence, the purchased galvanized iron sheet has the thickness of 1.22 mm, the thickness of the galvanized iron sheet is more than 0.02 mm, and the width of the galvanized iron sheet is 1 meter. And cutting the iron sheet roll into a plate with the length of 10 meters by using a plate cutting machine. A drill bit with a diameter of 6 mm is used to drill a hole in the iron sheet at a proper position so as to fix the iron sheet. In the vertical direction, angle iron and stainless steel screws (1-2 stainless steel washers are placed between a screw and an iron sheet and between nuts) with the width of 5 multiplied by 5 cm and the height of 2 m are used for fixing the iron sheet every 2 m, and stainless steel angle iron and stainless steel screws (stainless steel washers are placed between the screw and the iron sheet and between the screw and the nuts) with the width of 10 multiplied by 10 cm and the height of 2 m are used for fixing the iron sheet every 10 m; in the horizontal direction, 2 iron sheets are fixed by stainless steel flat irons with the width of 8 cm and stainless steel screws (stainless steel washers are arranged among screw rods, the iron sheets and nuts), so that the iron sheets are integrated. The enclosure is checked every 1 month to reinforce the loosened iron sheet.
Digging a trench with the depth of 50 cm and the width of 50 cm by using a small excavator, burying the iron sheet and the angle iron together into the ground for 0.5 m, and reserving the iron sheet on the ground for 1.5 m. Backfilling soil, planting seeds of the grassland native vegetation, and tamping the backfilled soil by using a tamping machine to prevent animals from digging and escaping. The depth of the underground buried iron sheet is determined by the physicochemical properties of local soil, the excavating capacity of experimental animals, the depth of a cave under natural conditions and the like. In the iron sheet fence, an artificial tunnel with the diameter of 15 cm and the distance of 30 cm from the ground is excavated every 10 m by using a tunnel excavator, so that a field artificial fence is constructed. The top of the fence is covered by a fishing net with 10 multiplied by 10 cm of grid to prevent predation of prey birds. The periphery of the iron sheet fence is surrounded by a net fence at a position 50 cm away from the iron sheet fence, so that interference of livestock is prevented. In order to reduce the influence of the reflection of the galvanized iron sheet on experimenters, the surrounding environment and experimental animals and prolong the service life of the iron sheet, green metallic paint or alkyd enamel paint, epoxy resin paint and the like are used, and are matched with a diluent and a curing agent and then sprayed on the iron sheet, the angle iron, the flat iron and the screw, and paint is sprayed for at least 2-3 times. After the paint dries, red paint is used to mark a number, 0-80, on each 2 m scale at a distance of 1 m from the ground, so as to record the coordinate position of the animal at a later stage. An observation platform with the height of 3 meters and the length and the width of 1 meter is built at the position 5 meters outside the fence, and can reach the top of the observation platform through the steps, so that long-term observation is facilitated.
In the 10 months, healthy adult rat rabbits are captured by using a live capture cage in grassland with a large population, at the peak of animal activity of 7:00-11:00 in the morning and 16:00-19:00 in the afternoon. Each individual is identified with sex, weighed, measured for body length, skull length and the like, and then the individual is doubly marked by using plastic ear tags with different colors and passive electronic tags; individuals are dyed by using a human black hair dye, male individuals are dyed with heads, and female individuals are dyed with backs, so that the movement conditions of the animals can be observed at a long distance. The marked individuals are independently placed into polypropylene plastic mouse cages of 200X 350X 475 mm (length X width X height) for rest for 15 minutes, and after the marked individuals return to normal, the marked individuals are lightly placed into a fence. Each pen contained 30 pens, 15 each male and female. The animals are placed in the fence for 1-7 days, at the peak of animal activity of 7:00-11:00 in the morning and 16:00-19:00 in the afternoon every day, the experimenter observes and records the animal activity condition by using a high-power telescope on an observation platform outside the fence; and (4) capturing all individuals again on the 7 th day, checking whether the ear tags and the passive electronic tags are intact, judging whether the health conditions of the individuals are good or not by weighing the body weight, measuring the body length and the skull length, observing the hair color, the activity ability and the like, and calculating the survival rate of the individuals within 7 days. The plateau rat rabbit firstly utilizes the excavated artificial tunnel and excavates a complex tunnel at the same time. Through observation and recapture examination for 7 days, the survival condition of the individual is good, and the survival rate within 7 days is over 95 percent, which indicates that the animal is suitable for the artificially constructed fence environment, and the related follow-up study can be carried out.
The above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited thereto, and any simple modifications or equivalent substitutions of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention are within the scope of the present invention.
Claims (2)
1. A method for breeding rodents in a field fence is characterized by comprising the following steps:
step 1, selecting a field natural grassland with flat terrain and good vegetation growth condition during 5-10 months, constructing an iron sheet fence with length multiplied by width of 60 multiplied by 80 meters, and cutting an iron sheet roll into a plate with the length of 10 meters by using a plate cutting machine; drilling holes in proper positions on the iron sheet by using a drill bit with the diameter of 4-6 mm so as to fix the iron sheet; in the vertical direction, angle iron and stainless steel screws with the width of 5 multiplied by 5 centimeters and the height of 2 meters are used for fixing iron sheets every 2 meters, 1-2 stainless steel gaskets are arranged between a screw and the iron sheets and between the screw and the nuts, stainless steel angle iron and stainless steel screws with the width of 10 multiplied by 10 centimeters and the height of 2 meters are used for fixing the iron sheets every 10 meters, and stainless steel gaskets are arranged between the screw and the iron sheets and between the screw and the nuts; in the horizontal direction, 2 iron sheets are fixed by stainless steel flat irons and stainless steel screws with the width of 8 centimeters, and stainless steel washers are arranged among the screws, the iron sheets and the nuts to enable the iron sheets to be integrated; checking the iron sheet fence every 1 month, and reinforcing the loosened iron sheet;
step 2, digging a trench with the depth of 50 cm and the width of 50 cm by using a small excavator, burying the iron sheet and the angle iron together into the ground for 0.5 m, and reserving 1.5 m of the iron sheet on the ground; backfilling soil, planting seeds of the grassland native vegetation, and tamping the backfilled soil by using a tamping machine to prevent animals from digging and escaping; the depth of the underground buried iron sheet is determined by the physicochemical property of local soil, the excavating capacity of experimental animals and the depth of a cave under natural conditions; digging artificial tunnels with the diameter of 15 cm and the distance of 30 cm from the ground every 10 m by using a tunnel excavator in the iron sheet fence to construct a field artificial fence; a fishing net with 10 multiplied by 10 cm of grid is used for covering the upper part of the artificial fence to prevent prey of prey birds; a net fence is used for surrounding the position 50 cm away from the periphery of the iron sheet fence to prevent and treat the interference of livestock; in order to reduce the influence of iron sheet reflection on experimenters, surrounding environment and experimental animals and prolong the service life of the iron sheet, green metallic paint or alkyd enamel paint and epoxy resin paint are used, and are sprayed on the iron sheet, angle iron, flat iron and screws after being matched with a diluent and a curing agent, and paint is sprayed for 2-3 times; after the paint is dried, marking a number within 0-60 at a position 1 m away from the ground by using red paint every 2 m on the iron sheet so as to record the coordinate position of the animal at a later stage; an observation platform with the height of 3 meters and the length and width of 1 meter is built at the position 3-5 meters outside the artificial fence, and can reach the top of the observation platform through the steps, so that long-term observation is facilitated;
step 3, in 5-10 months, capturing healthy adult rat rabbits by using a live capture cage in the grassland with a large population quantity of the rat rabbits in the plateau, at the peak period of animal activity of 7:00-11:00 in the morning and 16:00-19:00 in the afternoon; each individual is distinguished from sex, the weight is weighed, and after the body length and the skull length are measured, the individual is doubly marked by using plastic ear tags with different colors and a passive electronic tag; using a human black hair dye to dye individuals, dyeing the head of a male individual and dyeing the back of a female individual so as to observe the activity condition of an animal remotely; placing the marked individuals into a polypropylene plastic squirrel cage with the length, the width and the height of 200, 350 and 475 millimeters independently for rest for 15 minutes, and slightly placing an artificial fence after the marked individuals recover to be normal; each artificial fence is provided with 30 animals, and each animal is 15 animals; the animals are placed in the artificial fence for 1-7 days, at the peak of animal activity of 7:00-11:00 in the morning and 16:00-19:00 in the afternoon every day, the experimenter observes and records the animal activity condition by using a high-power telescope on an observation platform outside the artificial fence; re-capturing all individuals on day 7, checking whether ear tags and passive electronic tags are intact, judging whether the health conditions of the individuals are good or not by weighing the body weight, measuring the body length and the skull length, observing the hair color and the activity capacity, and calculating the survival rate of the individuals within 7 days; firstly, excavating an artificial tunnel by using the plateau rat rabbit, and excavating a complex tunnel; through observation and recapture examination for 7 days, the survival condition of the individual is good, and the survival rate within 7 days is over 95 percent, which indicates that the animal is suitable for the artificial fence environment constructed manually and relevant follow-up research is carried out.
2. The method of claim 1, wherein in step 1, the iron sheet is galvanized, the thickness of the iron sheet is 0.8-1.22 mm, the thickness of the galvanized layer is greater than 0.02 mm, and the width of the iron sheet is 1 m.
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CN2816235Y (en) * | 2005-07-22 | 2006-09-13 | 吉林时代绿源草产业有限公司 | Combined moveable railing |
CN201972456U (en) * | 2011-01-26 | 2011-09-14 | 河南省电力公司南阳供电公司 | Screen-type fireproof rail fence |
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CN203296470U (en) * | 2013-06-07 | 2013-11-20 | 中建四局第三建筑工程有限公司 | Finalization fence |
CN204804465U (en) * | 2015-06-30 | 2015-11-25 | 中国科学院昆明植物研究所 | Device is establish to ecological sample prescription rail |
CN106212367B (en) * | 2016-07-25 | 2019-04-19 | 上海福贝宠物用品有限公司 | A kind of booth and its cultural method for forest frog culture |
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2017
- 2017-01-11 CN CN201710019866.0A patent/CN107047453B/en not_active Expired - Fee Related
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