CN107047146A - A kind of utilization naringenin prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl - Google Patents
A kind of utilization naringenin prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl Download PDFInfo
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- CN107047146A CN107047146A CN201710304655.1A CN201710304655A CN107047146A CN 107047146 A CN107047146 A CN 107047146A CN 201710304655 A CN201710304655 A CN 201710304655A CN 107047146 A CN107047146 A CN 107047146A
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- China
- Prior art keywords
- naringenin
- bemisia tabaci
- leaf curl
- yellow leaf
- tomato yellow
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
Bemisia tabaci and the method for tomato yellow leaf curl are prevented and treated the invention discloses a kind of utilization naringenin, step includes：Naringenin is sprayed in plant leaf tow sides.At present, it is domestic that naringenin is not also applied to the relevant report that Bemisia tabaci is prevented and treated, the present invention selects nontoxic naringenin, it specify that naringenin controls the TYLCD of Bemisia tabaci and its propagation effect by field test and laboratory test, this method is compared with existing means of prevention, prevention effect is significantly increased, middle high toxicity pesticide control pest is instead of, effectively reduces environmental pollution.
Description
Technical field
The invention belongs to Agricultural pest control field, and in particular to one kind prevents and treats Bemisia tabaci and its propagation kind using naringenin
The method of eggplant tomato yellow leaf curl China.
Background technology
Bemisia tabaci Bemisia tabaci (Gennadius) are a kind of global important agricultural insects, endanger cruciate flower
Numerous crops such as section's class, solanaceous vegetables, melon, beans.Bemisia tabaci adults, nymph cause plant to decline by directly drawing water
It is weak, grow and be obstructed, and honeydew can be secreted induce mould dirty disease, and then influence the photosynthesis of plant leaf blade；Bemisia tabaci may be used also
Propagate various plants virosis and cause bigger indirect hazard.
As international trade contact is increasingly frequent, Bemisia tabaci spreads rapidly by flowers and other industrial crops nursery stocks,
Wide-scale distribution and population outbreak, and as the important pests in China's agricultural production all over the world.In addition, breaking out for Bemisia tabaci is led
Cause the generation of various plants virosis increasingly severe, wherein, tomato yellow leaf curl (Tomato Yellow Leaf Curl
Disease, TYLCD) propagated by bemisa babaci specificity, it is a kind of destructive disease in tomato production, tomato can be caused serious
Yield and quality loss.In the 1990s, TYLCD once had fragmentary generation in the area such as China Guangxi, Guangdong, Yunnan, but
It is that from 2005, TYLCD started large area in tomato producing regions such as the East China, south China, North China of China, before and after 2010,
TYLCD breaks out again in each province of China central and east, and indivedual serious areas even tomato total crop failure occur.In view of Bemisia tabaci and
It propagates being on the rise and its importance economically for TYLCD, seeks and effectively prevents and treats Bemisia tabaci and its propagate TYLCD strategy
Just seem with method particularly significant.
Research shows that the TYLCD propagation broken out with Bemisia tabaci is closely related, and the key measure that control TYLCD occurs is just
It is to prevent and treat Bemisia tabaci in time.Practice have shown that, some agriculturals and physical control measure are to preventing and controlling field Bemisia tabaci worm sources base
Number is very effective.But the preventing and treating to Bemisia tabaci relies primarily on chemical prevention at present, although chemical pesticide is to control Bemisia tabaci, reduction agriculture
The effect of industry economic loss is huge, but is largely also on the rise using Bemisia tabaci resistance problems caused by synthetic organic pesticide.
Due to the use of a large amount of chemical pesticides, Bemisia tabaci is more and more stronger to the resistance to the action of a drug of the medicaments such as organophosphor, pyrethroid, and agricultural chemicals is dirty
Dye seriously threatens food security, therefore, and how non-polluted food base Bemisia tabaci is into research emphasis in recent years.Plant secondary metabolites
Matter is possible to the frequent of solution chemical pesticide as a kind of plant protection product and uses brought pest resurgence, insect to agricultural chemicals
The resistance to the action of a drug and residual toxicity the problems such as, provide possibility from insect pest for plant.Flavone compound is largely present in
In higher plant, many flavone compounds isolated from plant have toxicity or Antifeedant Effects to insect, relatively more famous
Such as rutin sophorin, Quercetin and isoquercitrin.Naringenin is a kind of natural flavonoid compound, and its main component is that dihydro is yellow
Ketone, is widely present in Citrus paradisi Macfadyen, with the effect such as antibacterial, anti-inflammatory, anti-oxidant, removing free radical, and to people and family
No toxicity is raiseeed, is widely used in the fields such as medicine, food.
At present, it is domestic that naringenin is not also applied to the relevant report that Bemisia tabaci is prevented and treated.By the poisoning of naringenin or refuse
Food act on this thinking be incorporated into Bemisia tabaci control come up, it is possible to find a kind of new prophylactico-therapeutic measures, for find cigarette
Aleyrodid and its propagation TYLCD green prevention and control method are significant.
The content of the invention
In view of this, Bemisia tabaci and the method for tomato yellow leaf curl are prevented and treated the invention provides a kind of utilization naringenin,
This method more effectively can carry out non-environmental pollution control for Bemisia tabaci, be that Bemisia tabaci and its green prevention and control for propagating TYLCD provide one
Plant physical method.
First aspect present invention prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl there is provided a kind of utilization naringenin, step
Suddenly include：Naringenin is sprayed in plant leaf tow sides.
Second aspect of the present invention provides a kind of application of naringenin in Bemisia tabaci and tomato yellow leaf curl is prevented and treated, will
Naringenin is used to prevent and treat Bemisia tabaci and tomato yellow leaf curl..
The beneficial effects of the invention are as follows：1) compared with existing means of prevention, prevention effect is significantly increased, is had to Bemisia tabaci
There is obvious insecticidal effect, preventive effect reaches 92% or so after handling 1 day, after handling 7 days, anti-efficiency reaches more than 95%, to cigarette
Aleyrodid, which propagates TYLCD, also obvious inhibiting effect, and inhibiting rate can reach more than 80%；2) it instead of middle high toxicity pesticide control evil
Worm, effectively reduces environmental pollution, does not have toxicity to people and domestic animal, is a kind of Bemisia tabaci and its propagation TYLCD green prevention and control side
Method, has a good application prospect and Research Significance.
Embodiment
First aspect present invention provides and prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl, step bag using naringenin
Include：Naringenin is sprayed in plant leaf tow sides.
Naringenin has good preventive effect to Bemisia tabaci, and can effectively suppress transmission via whitefly TYLCD, the present invention's
By field and laboratory test in several embodiments, this effect of naringenin is specify that, and application process is simple, effect is bright
It is aobvious, with excellent application prospect.
It is preferred that, the naringenin, which is dissolved in ethanol solution, makes naringenin concentration be 3g/L~15g/L, is then sprayed
Apply.
More preferred, the naringenin, which is dissolved in ethanol solution, makes naringenin concentration be 5g/L~10g/L, then carries out
Spray.
It is preferred that, the ethanol solution is the aqueous solution for the ethanol that volume ratio is 1%.
It is preferred that, the dosage of the naringenin is 250g/ mus~500g/ mus.
It is preferred that, the plant is tomato plant.
Second aspect of the present invention provides a kind of application of naringenin in Bemisia tabaci and tomato yellow leaf curl is prevented and treated, will
Naringenin is used to prevent and treat Bemisia tabaci and tomato yellow leaf curl..
Naringenin is utilized to prevent and treat Bemisia tabaci and tomato yellowing one kind that the present invention is provided below in conjunction with specific embodiment
The method of leaf curl is further described.The embodiments described below is exemplary, is only used for explaining the present invention, and can not
It is interpreted as limitation of the present invention.
Experimental method in following embodiments, is conventional method unless otherwise specified.Reality used in following embodiments
Test material unless otherwise specified, be that market is commercially available.
Embodiment 1
The present embodiment determines preventive and therapeutic effect of the naringenin to Bemisia tabaci by field test, and specific experiment process is as follows：
Materials and methods：Reagent agent is naringenin.Naringenin is sprayed to the preventive and therapeutic effect of tomato Bemisia tabaci in 2017 3
The moon is carried out in Changjiang University of Jingzhou City of Hubei Province experiment greenhouse.Test as plot experiment, each cell 15m2, respectively handle and be all provided with 3
Individual replicated plot, and set blank control.Each cell mark 10 has the plant of Bemisia tabaci, counts the number of full leaf Bemisia tabaci
Amount, it is ensured that be more than 120 per cell Bemisia tabaci quantity.Naringenin is dissolved in 1% ethanol (body in 5g/L and 10g/L ratio
Product ratio) in solution, each reagent agent is imposed on into tomato leaf positive and negative two by conventional spray respectively using plastics knapsack sprayer
Face, is compared with 1% ethanol solution spraying.The Bemisia tabaci radix per cell is investigated before dispenser, respectively at after dispenser the 1st, 3,7
Bemisia tabaci work borer population amount on its investigation tomato leaf.The prevention effect of naringenin is calculated by following equation：
Wherein, Pt0：Borer population living, Pt1 before treatment region dispenser：Borer population living, CK0 after treatment region dispenser：Blank district dispenser
Preceding borer population living, CK1：Borer population living after blank district dispenser.
The effect for preventing and treating naringenin Bemisia tabaci using SPSS softwares and using Tukey methods carries out significance of difference statistical
Analysis.
Result of the test：As known from Table 1, naringenin has obvious insecticidal effect to Bemisia tabaci, after processing 1 day no matter
It is 5g/L or 10g/L naringenin solution energy effectively preventing Bemisia tabaci adults, preventive effect reaches 92% or so, same processing
After 3 days and 7 days, prevention effect is fine, and after especially handling 7 days, anti-efficiency reaches more than 95%.
The naringenin of table 1 prevents and treats tomato Bemisia tabaci field control effectiveness test result
Numeral is average value ± standard error in upper table, and lowercase is independent sample T inspections, small letter parent phase in same row
It is not notable in 0.05 level difference with the preventive effect after expression different agents concentration processing same time；It is with capitalization in a line
Single factor test variance test, the identical preventive effect represented after identical drug concentration processing different time of capitalization is in 0.05 level difference
Not significantly, similarly hereinafter.
Embodiment 2
The present embodiment passes through inhibitory action of the clear and definite naringenin of laboratory test to transmission via whitefly TYLCD, specific experiment mistake
Journey is as follows：
Materials and methods：Material to be tested is naringenin, is strong for studying thing for examination insect to carry TYLCD Bemisia tabaci
Health tomato.Test as laboratory test, 4 healthy tomato plants are placed in each insect cage (60cm × 40cm × 80cm), and (3 true
The leaf phase) as a repetition, each processing is all provided with 3 repetitions (insect cage), and sets blank control.Naringenin presses 5g/L and 10g/L
Ratio be dissolved in 1% ethanol (volume ratio) solution, using hand sprayer respectively by each reagent agent uniformly spray in
Healthy tomato leaf tow sides, are compared with 1% ethanol solution spraying.Randomly select 30 carrying TYLCD Bemisia tabaci
Female adult is discharged into the insect cage of each processing, and Bemisia tabaci is removed after taking food 48 hours, observes the incidence of tomato plant,
After 28 days the tomato plant quantity for determining to fall ill is detected according to Symptoms and by enzyme linked immunological (ELISA) kit.Press
Following equation calculates inhibition of the naringenin to transmission via whitefly TYLCD：
Wherein, CK：The susceptible number of tomato plant, Pt after blank district dispenser：The sense of tomato plant after treatment region dispenser
Sick number.
Result of the test：As known from Table 2, naringenin has obvious inhibitory action to transmission via whitefly TYLCD, either
5g/L or 10g/L naringenin solution can effectively suppress transmission via whitefly TYLCD, and inhibiting rate is more than 60%, especially
It is 10g/L naringenin, inhibiting rate reaches more than 80%.
The naringenin of table 2 suppresses transmission via whitefly TYLCD indoor pharmacodynamic test result
| Processing | Inhibiting rate (%) |
| 5g/L naringenins | 61.3±4.7b |
| 10g/L naringenins | 80.6±4.1a |
| Blank control | — |
The beneficial effects of the invention are as follows：1) compared with existing means of prevention, prevention effect is significantly increased, is had to Bemisia tabaci
There is obvious insecticidal effect, preventive effect reaches 92% or so after handling 1 day, after handling 7 days, anti-efficiency reaches more than 95%, to cigarette
Aleyrodid, which propagates TYLCD, also obvious inhibiting effect, and inhibiting rate can reach more than 80%；2) it instead of middle high toxicity pesticide control evil
Worm, effectively reduces environmental pollution, does not have toxicity to people and domestic animal, is a kind of Bemisia tabaci and its propagation TYLCD green prevention and control side
Method, has a good application prospect and Research Significance.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (7)
1. a kind of utilization naringenin prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl, it is characterised in that step includes：By shaddock
Pi Su is sprayed in plant leaf tow sides.
2. prevent and treat Bemisia tabaci and the method for tomato yellow leaf curl using naringenin as claimed in claim 1, it is characterised in that：
The naringenin, which is dissolved in ethanol solution, makes naringenin concentration be 3g/L~15g/L, is then sprayed.
3. utilization naringenin according to claim 2 prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl, its feature exists
In：The naringenin, which is dissolved in ethanol solution, makes naringenin concentration be 5g/L~10g/L, is then sprayed.
4. the utilization naringenin according to Claims 2 or 3 prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl, its feature
It is：The ethanol solution is the aqueous solution for the ethanol that volume ratio is 1%.
5. utilization naringenin according to claim 1 prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl, its feature exists
In：The dosage of the naringenin is 250g/ mus~500g/ mus.
6. utilization naringenin according to claim 1 prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl, its feature exists
In：The plant is tomato plant.
7. application of a kind of naringenin in Bemisia tabaci and tomato yellow leaf curl is prevented and treated, it is characterised in that be used for naringenin
Prevent and treat Bemisia tabaci and tomato yellow leaf curl.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710304655.1A CN107047146A (en) | 2017-05-03 | 2017-05-03 | A kind of utilization naringenin prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl |
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| CN201710304655.1A CN107047146A (en) | 2017-05-03 | 2017-05-03 | A kind of utilization naringenin prevents and treats Bemisia tabaci and the method for tomato yellow leaf curl |
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| CN107047146A true CN107047146A (en) | 2017-08-18 |
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2017
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| CN101715793A (en) * | 2009-10-30 | 2010-06-02 | 茹晖 | Ecological pesticide and preparation method thereof |
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Application publication date: 20170818 |