CN107047044A - A kind of method of oriental hybrid lily root growth under promotion Acid-Al stress - Google Patents
A kind of method of oriental hybrid lily root growth under promotion Acid-Al stress Download PDFInfo
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- CN107047044A CN107047044A CN201710428914.1A CN201710428914A CN107047044A CN 107047044 A CN107047044 A CN 107047044A CN 201710428914 A CN201710428914 A CN 201710428914A CN 107047044 A CN107047044 A CN 107047044A
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- stress
- seedling
- acid
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- hybrid lily
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses a kind of method of oriental hybrid lily root growth under promotion Acid-Al stress, belong to the production of flowers and plants and administrative skill field, comprise the following steps that：Lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C, the lilium oriental after processing is planted in nutritive cube；Nutritive cube is placed in growth cabinet and cultivated, it is 14h/10h to set the light in growth cabinet, dark cycle, intensity of illumination is 10000lx, and diurnal temperature is 25 DEG C/16 DEG C, and relative humidity is 70%, produces seedling；It is 4.5, Al that seedling is placed on into pH3+To carry out the stress of 5~15 days in 100umol/L nutrient solution, during which the seedling under Acid-Al stress was carried out to spray processing between 2~8mg/L heteroauxin with concentration every 2 days, the amount of spraying is 1~3mL.The method of oriental hybrid lily root growth under Acid-Al stress is promoted to be laid a good foundation to cultivate the lily new varieties of resistance to aluminium in the present invention, therefore, the method in the present invention is adapted in the production of flowers and plants and management domain promotion and application.
Description
Technical field
The present invention relates to oriental hybrid lily root system under the production of flowers and plants and administrative skill field, more particularly to a kind of promotion Acid-Al stress
The method of growth.
Background technology
Plant aluminum poison has turned into the focus of global biology and environmental science concern caused by soil acidification.Acid-Al stress is acid
Property soil limit plant growth main cause, at present the agricultural arable land in the world more than 40% endangered by Acid-Al stress, aluminium
The main root growth for suppressing plant, influence moisture and Nutrient Absorption, so as to influence plant growth and development.Auxin is the mankind
The class plant hormone found earliest, not only plays an important role in the normal growth of plant, but also participates in adjusting a variety of sides of body
Compel the growth and development of lower root system of plant.In recent years, heteroauxin (IAA) is found have close ties with the resistance of plant,
The ability of the stress such as plant resistant salt, heavy metal can be improved, promotes the growth of plant under environment stress.
Lily is one of big cut-flower in the world today foremost four, and its production and consumption is gradually incremented by.Lily originates in north
Hemisphere temperate zone, minority originates in Southern Hemisphere frigid zone and the torrid zone, is suitably grown in well-drained slightly acidic environment.At present, with sight
Reward plant studies the rare report of research of the different cultivars to Acid-Al stress physiological responses and resistance to Acid-Al stress mechanism as test material,
For the research of the molecule and physiological mechanism of further carrying out the resistance to aluminium of lily, the lily new varieties based theoretical of resistance to aluminium is cultivated.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of processing method is simple and can promote oriental hybrid lily under Acid-Al stress
The method of root growth, the present invention is by carrying out low-temperature treatment to lilium oriental and using certain density heteroauxin pair
Seedling carries out spraying processing realizing the growth of promotion oriental hybrid lily root system.
In order to solve the above-mentioned technical problem, the invention provides a kind of side of oriental hybrid lily root growth under promotion Acid-Al stress
Method, is comprised the following steps that：
(1) lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C, by the east after low-temperature treatment
Lily ball is planted in nutritive cube；
(2) nutritive cube that cultivation has lilium oriental is placed in growth cabinet and cultivated, and set in growth cabinet
Light, the dark cycle be 14h/10h, intensity of illumination is 10000lx, and diurnal temperature is 25 DEG C/16 DEG C, and relative humidity is 70%, i.e.,
Obtain seedling；
(3) it is 4.5, Al the seedling of gained in step (2) to be placed on into pH3+For in 100umol/L nutrient solution carry out 5~
The stress of 15 days, during which carried out spraying place between 2~8mg/L heteroauxin with concentration every 2 days to the seedling under Acid-Al stress
Reason, is sprayed using the method for foliage-spray to seedling, and the amount of spraying is 1~3mL.
Further, the kind of the oriental hybrid lily is rope nation kind.
Further, Al in the step (3)3+Silicon source be AlCl3·6H2O。
Further, the concentration of the heteroauxin is 6mg/L.
Further, the amount of spraying is 2mL.
Compared with prior art, the beneficial effect that the present invention is reached：
(1) seedling carries out spraying processing with 6mg/L heteroauxin during being coerced to seedling in the present invention, using leaf
The method that face is sprayed is sprayed to seedling, and the amount of spraying is 2mL；Used in the present invention concentration for 6mg/L heteroauxin to by
The seedling of Acid-Al stress is handled, and the processing can substantially reduce mda content in seedling, while also making the electrical conductivity of seedling
Minimum point has been reached, wherein, seedling is carried out to spray processing using 6mg/L heteroauxin, super oxygen in seedling can be improved
The content of thing mutase, so as to reduce cell membrane lipid peroxidation and the destroyed degree of plasma membrane.
(2) seedling by Acid-Al stress is sprayed after heteroauxin in the present invention, the content of superoxide dismutase in seedling
Presentation is fallen before, the trend risen afterwards, and the content of peroxidase is presented and first risen in seedling, rear downward trend；The result table
Bright, heteroauxin promotes Acid-Al stress seedlings root growth and development to be by improving peroxidase in Acid-Al stress seedling first
Content mitigates cell and injured by active oxygen ion come the root cells oxygen radical removed Acid-Al stress induction and accumulated, so that
Protect the stability of cellulose.
(3) lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C in the present invention, by oriental hybrid lily
It is to break the rest period of bulb that bulb, which carries out low-temperature treatment,；Seedling is sprayed using the method for foliage-spray in the present invention
Apply, foliage-spray can not only enable plant root to absorb nutrient, moreover it is possible to leaf and green branch is also absorbed nutrient.
Brief description of the drawings
The present invention is described in further detail below in conjunction with the drawings and the specific embodiments.
Fig. 1 is bar chart of the heteroauxin to oriental hybrid lily mda content under Acid-Al stress in the present invention；
Fig. 2 is bar chart of the heteroauxin to oriental hybrid lily electrical conductivity under Acid-Al stress in the present invention；
Fig. 3 is bar chart of the heteroauxin to oriental hybrid lily POD activity under Acid-Al stress in the present invention；
Fig. 4 is bar chart of the heteroauxin to oriental hybrid lily SOD activity under Acid-Al stress in the present invention.
Embodiment
Following examples are merely to illustrate the present invention, but are not limited to the scope of the present invention.
1 materials and methods
1.1 material
Selected Lilium oriental 'Sorbonne' kind is subjects, and field planting material is used as by all 14~16cm of footpath bulbs.
1.2 method
Lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C, by the east hundred after low-temperature treatment
Bulb is closed to plant in nutritive cube；The nutritive cube that cultivation has lilium oriental is placed in growth cabinet and cultivated, and sets artificial
Light, dark cycle in climate box are 14h/10h, and intensity of illumination is 10000lx, and diurnal temperature is 25 DEG C/16 DEG C, and relative humidity is
70%, produce seedling；It is 4.5, Al that seedling is placed on into pH3+For carried out respectively in 100umol/L nutrient solution 5 days by a definite date, 10
The stress of it and 15 days, during which carried out spraying place with the heteroauxin of various concentrations to the seedling under Acid-Al stress respectively every 2 days
Reason, is sprayed using the method for foliage-spray to seedling, and the amount of spraying is 2mL, the concentration of wherein heteroauxin is respectively 0,2,
4、6、8mg/L.One is set simultaneously without Al3+The control group of stress, sprays equivalent distilled water, each 3 repetitions of processing.
2 results and analysis
Influence of 2.1 heteroauxins to MDA under Acid-Al stress
MDA (MDA) is the product of Lipid peroxidation metabolism, is also the mark that damaged membrane hinders degree.As shown in figure 1, with
Control group is compared, and the content of the MDA of seedling has different degrees of raising in other groups, and the description of test Acid-Al stress causes
The peroxidization of cell membrane.Mda content is in addition Al in seedling3+Increase 68.6% than control group respectively afterwards,
38.1%th, 2.09%.As shown in figure 1, compared with control group, spraying the MDA in the seedling that indoleacetic acid content is 0mg/L
Content is higher than the mda content of seedling in control group, sprays the mda content in the seedling of other concentration heteroauxins than spray
The content that indoleacetic acid content is applied for the MDA in 0mg/L seedling is all low, and downward trend is presented；As heteroauxin is dense
Degree increases, and the mda content in seedling is gradually reduced, wherein, the content that 6mg/L sprays MDA in the seedling of processing is bright
The aobvious content for being less than the MDA of seedling in control group.
Influence of 2.2 heteroauxins to electrical conductivity under Acid-Al stress
As shown in Fig. 2 compared with control group, electrical conductivity of the seedling under Acid-Al stress increased.The electrical conductivity of seedling
In addition Al3+27.9%, 25.7%, 30.2% is increased than control group respectively afterwards, the experimental data illustrates the increase of electrical conductivity
With film function damage into positive correlation, can as environment stress efficiency index.In addition, the increase of heteroauxin, rises to membrane damage
Certain restitution has been arrived, and with the raising of heteroauxin concentration, becoming for reduction is also presented in the electrical conductivity of seedling substantially
Gesture, wherein, the electrical conductivity that 6mg/L sprays the seedling of processing has reached minimum point.
Influence of 2.3 heteroauxins to peroxidase content under Acid-Al stress
As shown in figure 3, lasting increasing is presented with the extension of Acid-Al stress time in the content of peroxidase (POD) in seedling
High trend, and be above no Acid-Al stress, amplification respectively up to 13.1%, 25.8%, 26.0%, the amplitude of increasing be also gradually on
Rise.This experimental result illustrates that Acid-Al stress promotes the formation of peroxidase and activity in seedling.As shown in figure 3, seedling is in aluminium
Stress is in the case of 5 days, and with the addition of heteroauxin, the content of peroxidase is being continuously increased in seedling；Work as Acid-Al stress
Time lengthening is to after 10 days, and the addition of heteroauxin reduces the content of peroxidase in seedling, and when handling 10 days, children
Peroxidase content reaches minimum in seedling, with the raising of heteroauxin concentration, and peroxidase, which is presented, in seedling declines
Trend.
Influence of 2.4 heteroauxins to Acid-Al stress superoxide dismutase content
As shown in figure 4, under Acid-Al stress, what the content presentation of superoxide dismutase (SOD) was fallen after rising in seedling becomes
Gesture.By superoxide dismutase content in the Acid-Al stress seedling of 10 days than by superoxide dismutase in the Acid-Al stress seedling of 5 days
Content increases 6.2%, by superoxide dismutase content in the Acid-Al stress seedling of 15 days than by the Acid-Al stress seedling of 10 days
Superoxide dismutase content reduces 10.8%；In the case of by Acid-Al stress 5 days, 10 days, 15 days, superoxides in seedling
The content of mutase be reduced to respectively the content of superoxide dismutase in no aluminium control group seedling 86.8%, 88.8%,
68.2%.The experimental data illustrates that Acid-Al stress reduces the content of superoxide dismutase in seedling, Acid-Al stress to some extent
Inhibit the activity of superoxide dismutase.When spraying the heteroauxin of low concentration, under the content of superoxide dismutase is in
Drop trend, but with the raising of heteroauxin concentration, the content of superoxide dismutase is presented significant difference, occurred in that significantly
That spends increases, wherein, when carrying out spraying processing 10 days, 15 days, shadow of the 6mg/L heteroauxins to superoxide dismutase content
Sound is the most notable.
Embodiment 1
The method of oriental hybrid lily root growth, is comprised the following steps that under a kind of promotion Acid-Al stress：
(1) lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C, by the east after low-temperature treatment
Lily ball is planted in nutritive cube；
(2) nutritive cube that cultivation has lilium oriental is placed in growth cabinet and cultivated, and set in growth cabinet
Light, the dark cycle be 14h/10h, intensity of illumination is 10000lx, and diurnal temperature is 25 DEG C/16 DEG C, and relative humidity is 70%, i.e.,
Obtain seedling；
(3) it is 4.5, Al the seedling of gained in step (2) to be placed on into pH3+To be carried out 5 days in 100umol/L nutrient solution
Stress, the heteroauxin for being during which 2mg/L with concentration every 2 days carries out spraying processing to the seedling under Acid-Al stress, using leaf
The method that face is sprayed is sprayed to seedling, and the amount of spraying is 1mL.
In the present embodiment, the kind of the oriental hybrid lily is rope nation kind.
In the present embodiment, Al in the step (3)3+Silicon source be AlCl3·6H2O。
Embodiment 2
The method of oriental hybrid lily root growth, is comprised the following steps that under a kind of promotion Acid-Al stress：
(1) lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C, by the east after low-temperature treatment
Lily ball is planted in nutritive cube；
(2) nutritive cube that cultivation has lilium oriental is placed in growth cabinet and cultivated, and set in growth cabinet
Light, the dark cycle be 14h/10h, intensity of illumination is 10000lx, and diurnal temperature is 25 DEG C/16 DEG C, and relative humidity is 70%, i.e.,
Obtain seedling；
(3) it is 4.5, Al the seedling of gained in step (2) to be placed on into pH3+To carry out 10 in 100umol/L nutrient solution
It stress, the heteroauxin for being during which 6mg/L with concentration every 2 days carries out spraying processing to the seedling under Acid-Al stress, uses
The method of foliage-spray is sprayed to seedling, and the amount of spraying is 2mL.
In the present embodiment, the kind of the oriental hybrid lily is rope nation kind.
In the present embodiment, Al in the step (3)3+Silicon source be AlCl3·6H2O。
Embodiment 3
The method of oriental hybrid lily root growth, is comprised the following steps that under a kind of promotion Acid-Al stress：
(1) lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C, by the east after low-temperature treatment
Lily ball is planted in nutritive cube；
(2) nutritive cube that cultivation has lilium oriental is placed in growth cabinet and cultivated, and set in growth cabinet
Light, the dark cycle be 14h/10h, intensity of illumination is 10000lx, and diurnal temperature is 25 DEG C/16 DEG C, and relative humidity is 70%, i.e.,
Obtain seedling；
(3) it is 4.5, Al the seedling of gained in step (2) to be placed on into pH3+To carry out 15 in 100umol/L nutrient solution
It stress, the heteroauxin for being during which 8mg/L with concentration every 2 days carries out spraying processing to the seedling under Acid-Al stress, uses
The method of foliage-spray is sprayed to seedling, and the amount of spraying is 3mL.
In the present embodiment, the kind of the oriental hybrid lily is rope nation kind.
In the present embodiment, Al in the step (3)3+Silicon source be AlCl3·6H2O。
In summary, understand that MDA contains in seedling from seedling by result from after Acid-Al stress 5 days, 10 days, 15 days
Amount is consistently higher than no Acid-Al stress, illustrates that Acid-Al stress generates obvious destruction to Seedlings Cell film；MDA contains in seedling
Amount growth rate is gradually reduced, and gap is less and less between control group, illustrates enhancing and work of the seedling by self-defensive system
The elimination of property oxygen, gradually alleviates injury of the Acid-Al stress to it.Under Acid-Al stress, the electrical conductivity of seedling is also consistently higher than no aluminium side of body
Compel, show that Acid-Al stress causes injury to the plasma structure of seedling, Electrolyte Leakage increase, cell plasma balance is affected,
And directly influence the growth of seedling.Acid-Al stress reduces the content of superoxide dismutase in seedling to some extent, the knot
Fruit shows that Acid-Al stress inhibits the synthesis of superoxide dismutase and activity in cell.The peroxidase of seedling contains under Acid-Al stress
The trend persistently increased is presented with the extension of Acid-Al stress time in amount, and is consistently higher than no Acid-Al stress, shows that Acid-Al stress promotees
Formation and the activity of the peroxidase of seedling are entered.Spray after heteroauxin, under the content of superoxide dismutase is presented first
Drop, the trend risen afterwards；The content of peroxidase, which is presented, first to be risen, rear downward trend；As a result show, heteroauxin promotes
Seedlings root growth and development is to remove aluminium by improving the content of peroxidase in Acid-Al stress seedling first under Acid-Al stress
Stress-inducing and the root cells oxygen radical of accumulation, mitigate cell and are injured by active oxygen ion, so as to protect cellulose
Stability.
Those of ordinary skill in the art it should be appreciated that the embodiment of the above be intended merely to explanation the present invention,
And be not used as limitation of the invention, as long as in the spirit of the present invention, the change to embodiment described above
Change, modification will all fall in scope of the presently claimed invention.
Claims (5)
1. a kind of method of oriental hybrid lily root growth under promotion Acid-Al stress, it is characterised in that comprise the following steps that：
(1) lilium oriental is placed in temperature to carry out low-temperature treatment under conditions of 2 DEG C, by the oriental hybrid lily after low-temperature treatment
Bulb is planted in nutritive cube；
(2) nutritive cube that has lilium oriental will be planted be placed in growth cabinet and cultivate, and set light in growth cabinet,
The dark cycle is 14h/10h, and intensity of illumination is 10000lx, and diurnal temperature is 25 DEG C/16 DEG C, and relative humidity is 70%, produces children
Seedling；
(3) it is 4.5, Al the seedling of gained in step (2) to be placed on into pH3+To be carried out 5~15 days in 100umol/L nutrient solution
Stress, during which the seedling under Acid-Al stress was carried out to spray processing between 2~8mg/L heteroauxin with concentration every 2 days, adopted
Seedling is sprayed with the method for foliage-spray, the amount of spraying is 1~3mL.
2. the method for oriental hybrid lily root growth under a kind of promotion Acid-Al stress according to claim 1, it is characterised in that institute
The kind for stating oriental hybrid lily is rope nation kind.
3. the method for oriental hybrid lily root growth under a kind of promotion Acid-Al stress according to claim 1, it is characterised in that institute
State Al in step (3)3+Silicon source be AlCl3·6H2O。
4. the method for oriental hybrid lily root growth under a kind of promotion Acid-Al stress according to claim 1, it is characterised in that institute
The concentration for stating heteroauxin is 6mg/L.
5. the method for oriental hybrid lily root growth under a kind of promotion Acid-Al stress according to claim 1, it is characterised in that institute
The amount of spraying is stated for 2mL.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115119693A (en) * | 2022-06-24 | 2022-09-30 | 广东省农业科学院蔬菜研究所 | Screening method of low-temperature-resistant cutting melons in seedling stage |
-
2017
- 2017-06-08 CN CN201710428914.1A patent/CN107047044A/en active Pending
Non-Patent Citations (2)
| Title |
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| 徐琼等: "低温处理对观赏百合种球碳水化合物及蛋白质代谢的影响 ", 《甘肃农业大学学报》 * |
| 闫雯等: "二种生长调节剂对紫花苜蓿铝胁迫和恢复生长的影响 ", 《上海交通大学学报(农业科学版)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115119693A (en) * | 2022-06-24 | 2022-09-30 | 广东省农业科学院蔬菜研究所 | Screening method of low-temperature-resistant cutting melons in seedling stage |
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