CN107044959B - Micro- multi-modal fusion spectral detection system - Google Patents
Micro- multi-modal fusion spectral detection system Download PDFInfo
- Publication number
- CN107044959B CN107044959B CN201710082623.1A CN201710082623A CN107044959B CN 107044959 B CN107044959 B CN 107044959B CN 201710082623 A CN201710082623 A CN 201710082623A CN 107044959 B CN107044959 B CN 107044959B
- Authority
- CN
- China
- Prior art keywords
- module
- mirror
- reflecting
- light
- spectrum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/066—Modifiable path; multiple paths in one sample
- G01N2201/0668—Multiple paths; optimisable path length
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/068—Optics, miscellaneous
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention discloses a kind of micro- multi-modal fusion spectral detection system, belongs to technical field of optical detection；The system comprises Raman spectrum module, fluorescence spectrum module, near infrared spectrum module, optical microphotograph module, optics regulation module, computer and acquisition control softwares；Regulate and control module by optics, light progress optical path modulation is issued to light source group in each spectroscopic module and channel is closed, through diffusing reflection or transmission light echo modulation module after optical transport to tested point, is respectively transmitted to each spectrum acquisition module and obtains the spectrum of object to be measured；The spectrum for obtaining the multiple modalities of object to be measured with position under micro-scale guarantees fining and homology that multi-modal spectral information obtains；The present invention overcomes the problems, such as that single spectrum detection technique obtains information and do not cause detection accuracy not high comprehensively, overcomes the problems, such as that multi-source optical spectrum integration technology pickup area is unmatched, realizes message complementary sense from molecular spectrum securing mechanism.
Description
Technical field
The present invention relates to a kind of micro- multi-modal fusion spectral detection systems, belong to technical field of optical detection.
Background technique
Speed is fast, high-efficient, at low cost, favorable reproducibility, does not destroy sample and pollution-free etc. because having for spectral analysis technique
Advantage is widely used to the multiple fields such as food, agricultural, medicine and chemical industry.Spectral analysis technique has become a kind of quick, nothing
The modern analytical technique of damage.Substance is carried out using the emission spectrum of substance, absorption spectrum or scattering spectrum feature qualitative or fixed
Amount analysis.In molecular spectrum, near infrared spectrum detection is vibration caused by electric dipole moment variation, and Raman spectroscopic detection is point
Vibration caused by muon polarization, and fluorescence spectrum reflects the molecular information with long conjugated structure, it is seen that the light under different modalities
Spectrum information is with their own characteristics.
Single detection means tends not to comprehensively obtain information, can only describe one of aspect, this to stress
The different bring limitations of point necessarily affect the precision and stability of testing result.In order to improve detection accuracy, accuracy, steady
It qualitatively requires, multiple technologies means is comprehensively considered, the detection information of multiple technologies is analyzed, merges peace
Weighing apparatus, while the new concept, new method and new Data Analysis Services technical application that introduce nonlinear science merge and know to information
In the foundation of other mode.
Domestic and foreign literature retrieval shows that in terms of multi-sensor information fusion, publication number CN101551341 " is based on image
With the meat online non-destructive testing method and device of Spectrum Data Fusion ", the CN101718682A " birds, beasts and eggs based on multi-information fusion
Freshness online test method and device, a kind of CN103278609A " Meat inspection based on the fusion of multi-source perception information
Survey method ", CN103472197A " across the perception information interaction in a kind of bionical evaluation of Intelligent Food incudes fusion method " etc.
Patent of invention, fusion utilizes different dimensions, the sensor information of magnitude, but different sensors acquisition of information position is it is difficult to ensure that one
It causes, the precision of detection will be directly affected；A kind of publication number CN102818777A " fruit maturity based on spectrum and color measuring
The hairs such as evaluation method ", CN103163083A " a kind of the two waveband spectrum fusion method and system of fresh meat multi-parameter detection "
The uniformity signal that bright patent utilizes same position to obtain carries out comprehensive analysis processing to improve detection accuracy.
Information fusion technology makes a variety of sensing technology integrations, not only displays one's respective advantages, but also accomplish message complementary sense.Multiple
In the case that detection field, especially detection accuracy require height and detection limit relatively low, multi-source sensor information fusion technology is shown
Impayable advantage.But there is both sides bottleneck problem in information fusion technology, first is that the homologous acquisition of information, it is desirable that no
What same sensor obtained is the information of sample the same area or position, guarantees the homology and information fusion of acquisition of information
Data foundation stone；Second is that the complementarity of information, it is desirable that different heat transfer agents reflect not belonging to for object same area to be measured respectively
Property or feature are able to achieve mutually compensating between information.If it is more to can be realized near infrared spectrum, Raman spectrum, fluorescence spectrum etc.
The homologous acquisition of fining of kind mode molecular spectrum information, must have qualitative leap in detectability and precision aspect.In this regard, this hair
It is bright to provide a kind of micro- multi-modal fusion spectral detection system.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of micro- multi-modal fusion spectral detection system, solves multimode
State spectral information refines homologous acquisition problem, realizes the homologous matching of different molecular spectral information, and then choose different modalities
The spectral information of lower most worthy is merged, and the good detection model of precision high stability is established, improve detection accuracy and
Sensitivity.
To achieve the above object, the present invention provides a kind of micro- multi-modal fusion spectral detection system, the system comprises
Raman spectrum module, fluorescence spectrum module, near infrared spectrum module, optical microphotograph module, optics regulate and control module, computer and adopt
Collection control software；It is designed using multi-pass with axiation, by optical modulation module, light is issued to light source in each spectroscopic module and is carried out
Optical path modulation and channel closure, light are returned after the main optical path of optical microphotograph module is transferred to tested point with diffusing reflection or transmission
Optical modulation module is successively transferred to each spectrum acquisition module and obtains the spectrum of object to be measured, realizes the same district under micro-scale
Position obtains the spectrum of the multiple modalities of object to be measured, guarantees fining and homology that multi-modal spectral information obtains.
The Raman spectrum module is used to obtain the micro Raman spectra of object to be measured；The Raman spectrum module includes swashing
Light device, beam expander, collimating mirror one, laser line filter, reflecting mirror one, dichroic mirror one, focus lamp one, fiber coupler one and drawing
Graceful spectrometer；The light that laser issues becomes collimated light beam by collimating mirror one again after beam expander expands, and filters through laser rays
Piece filters out stray light, reflects light to dichroic mirror one through reflecting mirror one, then reflect through dichroic mirror one and half-reflecting half mirror two, passes through
Object lens are transferred to sample surface, and sample diffuses by the way that after object lens to half-reflecting half mirror two, a part of light penetrates and passes to camera,
A part of light is reflected into dichroic mirror one, and line focus mirror one enters fiber coupler one, obtains finally by Raman spectrometer micro-
Raman spectrum.
The fluorescence spectrum module is used to obtain the micro-fluorescence spectrum of object to be measured；The fluorescence spectrum module includes purple
Outer lamp, collimating mirror two, reflecting mirror two, dichroic mirror two, long wave pass filter, focus lamp two, fiber coupler two and fluorescence spectrum
Instrument；The collimated mirror two of light that ultraviolet lamp issues becomes collimated light beam, is reflected on dichroic mirror two through reflecting mirror two, then through dichroic mirror
Two and half-reflecting half mirror three reflect, be transferred to sample surface by object lens, sample diffuses through object lens to half-reflecting half mirror
After three, through camera is passed to, a part of light is reflected into dichroic mirror two for a part of light, eliminates exciting light and miscellaneous through long wave pass filter
Astigmatism, then line focus mirror two enters fiber coupler two, obtains micro-fluorescence spectrum finally by Fluorescence Spectrometer.
The near infrared spectrum module is used to obtain the micro- near infrared spectrum of object to be measured；The near infrared spectrum module
Including tungsten halogen lamp, collimating mirror three, half-reflecting half mirror one, focus lamp three, fiber coupler three and near infrared spectrometer；Tungsten halogen lamp hair
The collimated mirror three of light out is transferred in the primary optical axis of optical microphotograph module after collimating, and is passed after half-reflecting half mirror four by object lens
Defeated to arrive sample surface, sample diffuses through object lens, half-reflecting half mirror four, and after half-reflecting half mirror five, a part of light is incoming
Camera, for acquiring image, a part is divided into two-way by half-reflecting half mirror one after reflection, and a part of line focus mirror three enters
Fiber coupler three obtains micro- near infrared spectrum finally by near infrared spectrometer；Another way passes to eyepiece through reflecting mirror three,
Sampled point is determined by eyepiece before acquisition spectrum.It is the optical microphotograph module and Raman spectrum module, fluorescence spectrum module, close red
External spectrum module organic linking, by optic path and control, for obtaining the spectral information under object micro-scale to be measured；Institute
Stating optical microphotograph module includes precision three-dimensional mobile platform, light box, object lens, half-reflecting half mirror two, half-reflecting half mirror three, half anti-half
Lens four, half-reflecting half mirror five, focus lamp four, camera, reflecting mirror three and eyepiece；The precision three-dimensional mobile platform is for precision
The region and position to be measured for adjusting sample, are placed in light box；The light box for shield external stray light enter it is described micro- more
Modality fusion spectral detection system；The camera is used for capturing sample image；When sample collection position is adjusted, seen by eyepiece
The morphologic localization feature for examining the sample areas of acquisition determines optimal spectra collection point.
Optical component object lens in optical microphotograph module, half-reflecting half mirror two, half-reflecting half mirror three, half-reflecting half mirror four,
The center line of half-reflecting half mirror five is primary optical axis；Spectrum obtains module (Raman spectrum module, fluorescence spectrum module and near infrared light
Spectrum module) it is the optical signal for issuing active light source and receiving sample return, the light that spectrum obtains in module will enter optical microphotograph
Primary optical axis realizes optical transport.
The optics regulates and controls module, controls for optical path modulation and optical device；Regulate and control module by the optics, to institute
It states light emitting source sending light progress optical path modulation and channel in each spectroscopic module to be closed, through diffusing reflection or thoroughly after optical transport to tested point
It penetrates in the primary optical axis for being transmitted back to optical microphotograph module, module is then regulated and controled by optics and carries out optical path modulation and channel closure, point
It is not transferred to each spectrum acquisition module and obtains the microspectrum of object to be measured；The optics regulation module is according to acquisition control software
The parameter of middle setting is transmitted instruction control by connection and is closed by the optical channel of half-reflecting half mirror, specific control insertion half
The shutter of anti-pellicle mirror executes channel closure；Spectral measurement parameter presets carry out optical path modulation automatically by software and channel is closed
It closes, the automatic homologous spectral information for realizing high quality obtains.
The computer connects Raman spectrometer in Raman spectrum module, glimmering in fluorescence spectrum module by data line
Near infrared spectrometer in photothermal spectroscopic analyzer, near infrared spectrum module is separately connected the phase in optical microphotograph module by connection
Machine and optics regulate and control module.
The acquisition control software installation is in computer, for system parameter setting and the module information of the front 5
Interaction, spectrum data gathering are shown to be handled with analysis.
As a preferred technical solution of the present invention, the laser is equipped with the raman excitation light of three emission sources simultaneously
Source, wavelength are respectively 532 nm, 785 nm and 1064 nm, cover from visible light near infrared spectrum, are joined according to Raman spectrum
Optical maser wavelength is automatically switched in number setting.
As a preferred technical solution of the present invention, the Raman spectrometer uses the hologram diffraction light of high-diffraction efficiency
Grid improve low-light detectability as dispersion element, by transmission microscope group, using back lighting type CCD/InGaAs of hypersensitivity
Detector array is as detecting element, so as to high speed, the measurement of parallel and continuous micro Raman spectra.
As a preferred technical solution of the present invention, it is 365 nm, configuration 400 that the ultraviolet lamp, which selects excitation wavelength,
Nm long wave pass filter avoids exciting light and stray light from entering Fluorescence Spectrometer；Preferably Fluorescence Spectrometer uses low noise
Electronic device and 18 A/D converters, select the slim ccd array of back-illuminated, and carry out semiconductor refrigerating, obtain high noise
Than improving the sensitivity of fluorescence detection, keeping the detection limit of fluorescence detection lower, improve the range and ability of detection.
Half-reflecting half mirror as a preferred technical solution of the present invention, in the primary optical axis of the optical microphotograph module
(including half-reflecting half mirror two, half-reflecting half mirror three, half-reflecting half mirror four, half-reflecting half mirror five) is embedded in optical channel closure substrate
On, shutter execution unit is controlled by controller and executes substrate closure；The closure substrate shares two states, first is that and optics
The primary optical axis of micro- module is parallel, while vertical with spectrum acquisition module primary optical axis, i.e., 0, this time can all pass through, and two
Parallel with the primary optical axis of optical microphotograph module and spectrum to obtain module primary optical axis be in 45 °, i.e., 1, the at this moment half-reflection and half-transmission
Mirror is in working position.The closure substrate is the movable part of intermediate aperture, for assembling the half-reflecting half mirror, and and shutter
Substrate condition adjustment is realized in execution unit combination.The shutter execution unit is mechanical shutter, electronic shutter, combination polarization tune
Device or spatial light modulation device processed.
As a preferred technical solution of the present invention, the optics regulation module realizes micro- multimode by following steps
The acquisition of state spectral information:
S1. the micro- multi-modal fusion spectral detection system carries out equipment initialization, and spectra collection is then arranged and joins
Number adjusts precision three-dimensional mobile platform to suitable sample from eyepiece and adopts such as light source mode, the time of integration, spectral resolution
Collect position；
When S2. acquiring Raman spectrum, the controller automatic control half-reflecting half mirror two in module is regulated and controled by optics and is in
1, half-reflecting half mirror three be in 0, half-reflecting half mirror four be in 0, half-reflecting half mirror five be in 1, half-reflecting half mirror one
In 1, half-reflecting half mirror three and half-reflecting half mirror four closes off fluorescence spectrum module and the light of near infrared spectrum module is defeated
Channel out avoids the attenuation by absorption of light disturbance and optical transmission process；
When S3. acquiring fluorescence spectrum, the controller automatic control half-reflecting half mirror two in module is regulated and controled by optics and is in
0, half-reflecting half mirror three be in 1, half-reflecting half mirror four be in 0, half-reflecting half mirror five be in 1, half-reflecting half mirror one
In 1, half-reflecting half mirror two and half-reflecting half mirror four closes off Raman spectrum module and the light of near infrared spectrum module is defeated
Channel out avoids the attenuation by absorption of light disturbance and optical transmission process；
S4. near infrared light time spectrum is acquired, the controller in module is regulated and controled by optics and is automatically controlled at half-reflecting half mirror two
1, half-reflecting half mirror are in 1, half-reflecting half mirror five in 0, half-reflecting half mirror four in 0, half-reflecting half mirror three
One is in 0, half-reflecting half mirror two and half-reflecting half mirror three closes off Raman spectrum module and the light of fluorescence spectrum module is defeated
Channel out avoids the attenuation by absorption of light disturbance and optical transmission process；
S5. by parameter setting, optics regulates and controls module and automatically controls successively acquisition micro Raman spectra, microscopic fluorescence light
Spectrum and micro- near infrared spectrum are transferred to after computer is used for respectively and by incoming camera acquired image by data line
Continuous processing.
As a preferred technical solution of the present invention, the acquisition control software is real using Visual Studio programming
It is existing, by the Software Development Kit for calling each spectrometer supplier to provide, realize the information exchange with each spectrometer；It is described to adopt
Collection controls every control instruction of Integrated Simulation optics regulation module and light source switch；Display circle of the acquisition control software
The spectrogram that face can be acquired with real-time display, built-in form selection, spectrum baseline correction, Pretreated spectra, clustering, mode
The functions such as identification and calibration model foundation；The acquisition control software development environment is 7 Ultimate (32 of computer system Windows
Position), 2010 Ultimate of Visual Studio, the executable file of the acquisition control software of exploitation, the executable text
Part may be mounted at the computer or industrial personal computer for meeting minimalist configuration, have preferable portable and compatibility.
The beneficial effects of the present invention are:
Compared with the prior art, the present invention proposes a kind of micro- multi-modal fusion spectral detection system, is regulated and controled by optics
Module organically combines Raman spectrum, fluorescence spectrum and near infrared spectrum and microscope equipment, realizes microspectrum under multiple modalities
Homologous acquisition, the homologous acquisition of information and information matches and complementary two big bottlenecks for solving multispectral information fusion technology ask
Topic guarantees fining and homology that multi-modal spectral information obtains, has qualitative leap in detectability and precision aspect；This hair
It is bright to overcome the problems, such as that single spectrum detection technique obtains information and do not cause detection accuracy not high comprehensively, overcome multi-source optical spectrum to merge skill
The unmatched problem of art pickup area, realizes message complementary sense from molecular spectrum securing mechanism.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is the present invention
Some embodiments for those of ordinary skill in the art without creative efforts, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 shows micro- multi-modal fusion spectral detection system structural schematic diagram；1 it is Raman spectrum module in figure, 2 is
Fluorescence spectrum module, 3 be near infrared spectrum module, 4 be optical microphotograph module, 5 be optics regulation module, 6 be that computer and 7 is
Acquisition control software.
Fig. 2 shows micro- multi-modal fusion spectral detection system optical transmission process schematic diagrames；Raman spectrum module in figure
Including laser (101), beam expander (102), collimating mirror one (103), laser line filter (104), reflecting mirror one (105), two
Look mirror one (106), focus lamp one (107), fiber coupler one (108) and Raman spectrometer (109)；Fluorescence spectrum module includes
Ultraviolet lamp (201), reflecting mirror two (203), dichroic mirror two (204), long wave pass filter (205), focuses collimating mirror two (202)
Mirror two (206), fiber coupler two (207) and Fluorescence Spectrometer (208)；Near infrared spectrum module includes tungsten halogen lamp (301), standard
Straight mirror three (302), half-reflecting half mirror one (303), focus lamp three (304), fiber coupler three (305) and near infrared spectrometer
(306)；Optical microphotograph module includes precision three-dimensional mobile platform (401), light box (402), object lens (501), half-reflecting half mirror two
(502), half-reflecting half mirror three (503), half-reflecting half mirror four (504), half-reflecting half mirror five (505), focus lamp four (506), phase
Machine (507), reflecting mirror three (508) and eyepiece (509).
Fig. 3 shows micro- multi-modal fusion spectral detection model foundation process schematic.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical solution in the embodiment of the present invention is explicitly described, it is clear that described embodiment is the present invention
A part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawings of the specification.
Embodiment 1:
As depicted in figs. 1 and 2, the present invention devises a kind of micro- multi-modal fusion spectral detection system, the system packet
Include Raman spectrum module 1, fluorescence spectrum module 2, near infrared spectrum module 3, optical microphotograph module 4, optics regulation module 5, meter
Calculation machine 6 and acquisition control software 7；It is designed using multi-pass with axiation, by optical modulation module, to light source in each spectroscopic module
Issue light and carry out optical path modulation and channel closure, light after the main optical path of optical microphotograph module is transferred to tested point with diffusing reflection or
Transmission light echo modulation module is successively transferred to each spectrum acquisition module and obtains the spectrum of object to be measured, realizes micro-
The spectrum for obtaining the multiple modalities of object to be measured under scale with position guarantees fining that multi-modal spectral information obtains and homologous
Property.
The Raman spectrum module 1 is used to obtain the micro Raman spectra of object to be measured；The Raman spectrum module 1 includes
Laser 101, collimating mirror 1, laser line filter 104, reflecting mirror 1, dichroic mirror 1, focuses beam expander 102
Mirror 1, fiber coupler 1 and Raman spectrometer 109；The light that laser 101 issues leads to again after beam expander 102 expands
Crossing collimating mirror 1 becomes collimated light beam, filters out stray light through laser line filter 104, reflects light to through reflecting mirror 1
Dichroic mirror one, then reflected through dichroic mirror 1 and half-reflecting half mirror 2 502, sample surface, sample are transferred to by object lens 501
It diffuses by the way that after object lens 501 to half-reflecting half mirror 2 502, through camera 507 is passed to, a part of light is reflected into a part of light
Dichroic mirror 1, line focus mirror 1 enter fiber coupler 1, obtain micro- drawing finally by Raman spectrometer 109
Graceful spectrum.
The fluorescence spectrum module 2 is used to obtain the micro-fluorescence spectrum of object to be measured；The fluorescence spectrum module 1 includes
Ultraviolet lamp 201, collimating mirror 2 202, reflecting mirror 2 203, dichroic mirror 2 204, long wave pass filter 205, focus lamp 2 206, light
Fine coupler 2 207 and Fluorescence Spectrometer 208；The collimated mirror 2 202 of light that ultraviolet lamp 201 issues becomes collimated light beam, through anti-
It penetrates mirror 2 203 to be reflected on dichroic mirror two, then is reflected through dichroic mirror 2 204 and half-reflecting half mirror 3 503, passed by object lens 501
Defeated to arrive sample surface, sample diffuses by the way that after object lens 501 to half-reflecting half mirror 3 503, a part of light penetrates and passes to camera
507, a part of light is reflected into dichroic mirror 2 204, eliminates exciting light and stray light, then line focus through long wave pass filter 205
Mirror 2 206 enters fiber coupler 2 207, obtains micro-fluorescence spectrum finally by Fluorescence Spectrometer 208.
The near infrared spectrum module 3 is used to obtain the micro- near infrared spectrum of object to be measured；The near infrared spectrum mould
Block 3 includes tungsten halogen lamp 301, collimating mirror 3 302, half-reflecting half mirror 1, focus lamp 3 304, fiber coupler 3 305 and close
Infrared spectrometer 306；The collimated mirror 3 302 of the light that tungsten halogen lamp 301 issues is transferred to the primary optical axis of optical microphotograph module 4 after collimating
In, it is transferred to sample surface by object lens 501 after half-reflecting half mirror 4 504, sample diffuses through object lens 501, half instead
Pellicle mirror 4 504, after half-reflecting half mirror 5 505, a part of light is passed to camera, and for acquiring image, a part is after reflection
It is divided into two-way by half-reflecting half mirror 1, a part of line focus mirror 3 304 enters fiber coupler 3 305, finally by close
Infrared spectrometer 306 obtains micro- near infrared spectrum；Another way passes to eyepiece through reflecting mirror 3 508, passes through mesh before acquiring spectrum
Mirror determines sampled point.
The optical microphotograph module 4 and Raman spectrum module 1, fluorescence spectrum module 2, the organic company of near infrared spectrum module 3
It connects, by optic path and control, for obtaining the spectral information under object micro-scale to be measured；The optical microphotograph module 4
Instead including precision three-dimensional mobile platform 401, light box 402, object lens 501, half-reflecting half mirror 2 502, half-reflecting half mirror 3 503, half
Pellicle mirror 4 504, half-reflecting half mirror 5 505, focus lamp 4 506, camera 507, reflecting mirror 3 508 and eyepiece 509；The precision
Three-dimensional mobile platform 401 is used for the region and position to be measured of fine adjustment sample, is placed in light box；The light box 402 is for shielding
External stray light is covered into the micro- multi-modal fusion spectral detection system；The camera 507 is used for capturing sample image；?
When sample collection position is adjusted, by the morphologic localization feature of the sample areas of the observation acquisition of eyepiece 509, optimal spectrum is determined
Collection point.
It is optical component object lens 501 in optical microphotograph module 4, half-reflecting half mirror 2 502, half-reflecting half mirror 3 503, half anti-
Pellicle mirror 4 504, half-reflecting half mirror 5 505 center line be primary optical axis；Spectrum obtains module (Raman spectrum module 1, fluorescence light
Spectrum module 2 and near infrared spectrum module 3) it is the optical signal for issuing active light source and receiving sample return, spectrum obtains in module
Light will enter optical microphotograph main optical path realize optical transport.
The optics regulates and controls module 5, controls for optical path modulation and optical device；Regulate and control module 5 by the optics, it is right
Light emitting source issues light and carries out optical path modulation and channel closure in each spectroscopic module, after optical transport to tested point through diffusing reflection or
Transmission returns in the primary optical axis of optical microphotograph module 4, then regulates and controls module by optics and carries out optical path modulation and channel closure,
It is respectively transmitted to each spectrum acquisition module and obtains the microspectrum of object to be measured；The optics regulation module 5 is according to acquisition control
The parameter set in software 7 executes channel by the shutter that connection transmitting instruction to controller controls insertion half-reflecting half mirror
Closure；Spectral measurement parameter presets carry out optical path modulation and channel closure by software automatically, automatic to realize the same of high quality
Source spectral information obtains.
The computer 6 connects Raman spectrometer in Raman spectrum module 1 by data line, in fluorescence spectrum module
Near infrared spectrometer 3 in Fluorescence Spectrometer 2, near infrared spectrum module, is separately connected in optical microphotograph module 4 by connection
Camera and optics regulate and control module 5.
The acquisition control software 7 is installed in computer 6, is believed for system parameter setting, with the module of the front 5
Breath interaction, spectrum data gathering are shown to be handled with analysis.
As a preferred technical solution of the present invention, the Raman that the laser 101 is equipped with three emission sources simultaneously swashs
Light emitting source, wavelength are respectively 532 nm, 785 nm and 1064 nm, are covered from visible light near infrared spectrum, according to Raman light
It composes parameter setting and automatically switches optical maser wavelength.
As a preferred technical solution of the present invention, the Raman spectrometer 109 is spread out using the holography of high-diffraction efficiency
Grating is penetrated as dispersion element, improves low-light detectability by transmission microscope group, using hypersensitivity back lighting type CCD/
InGaAs detector array is as detecting element, so as to high speed, the measurement of parallel and continuous micro Raman spectra；The implementation
The Raman spectrometer of example selects marine optics QE65 Pro type Raman spectrometer.
As a preferred technical solution of the present invention, it is 365 nm, configuration that the ultraviolet lamp 201, which selects excitation wavelength,
400 nm long wave pass filters, avoid exciting light and stray light from entering Fluorescence Spectrometer；Preferably Fluorescence Spectrometer 208 uses
The electronic device of low noise and 18 A/D converters select the slim ccd array of back-illuminated, and carry out semiconductor refrigerating, obtain high
Signal-to-noise ratio improves the sensitivity of fluorescence detection, keeps the detection limit of fluorescence detection lower, improves the range and ability of detection；The reality
The Fluorescence Spectrometer for applying example selects marine optics Maya2000 Pro type Fluorescence Spectrometer.
Half-reflecting half mirror as a preferred technical solution of the present invention, in the primary optical axis of the optical microphotograph module
(including half-reflecting half mirror 2 502, half-reflecting half mirror 3 503, half-reflecting half mirror 4 504, half-reflecting half mirror 5 505) is embedded in light
Channel is closed on substrate, is controlled shutter execution unit by controller and is executed substrate closure；There are two types of shapes altogether for the closure substrate
State, first is that parallel with the primary optical axis of optical microphotograph module while vertical with spectrum acquisition module primary optical axis, i.e., 0, this time can
All to pass through, second is that in 45 °, i.e., 1, at this moment and spectrum parallel with the primary optical axis of optical microphotograph module obtains module primary optical axis
The half-reflecting half mirror is in working position.The closure substrate is the movable part of intermediate aperture, for assembling described half anti-half
Lens, and combined with shutter execution unit and realize substrate condition adjustment.The shutter execution unit is mechanical shutter, electronics is fast
Door, combination Polarization Modulation device or spatial light modulation device.
As a preferred technical solution of the present invention, the optics regulation module 5 is realized micro- more by following steps
The acquisition of mode spectra information:
S1. the micro- multi-modal fusion spectral detection system carries out equipment initialization, and spectra collection is then arranged and joins
Number adjusts precision three-dimensional mobile platform to suitable sample from eyepiece and adopts such as light source mode, the time of integration, spectral resolution
Collect position；
When S2. acquiring Raman spectrum, the controller in module 5 is regulated and controled by optics and automatically controls half-reflecting half mirror 2 502
In 1, half-reflecting half mirror 3 503 be in 0, half-reflecting half mirror 4 504 be in 0, half-reflecting half mirror 5 505 be in 1,
Half-reflecting half mirror 1 is in 1, half-reflecting half mirror 3 503 and half-reflecting half mirror 4 504 close off fluorescence spectrum module and
The light output channel of near infrared spectrum module, avoids the attenuation by absorption of light disturbance and optical transmission process；
When S3. acquiring fluorescence spectrum, the controller in module 5 is regulated and controled by optics and automatically controls half-reflecting half mirror 2 502
In 0, half-reflecting half mirror 3 503 be in 1, half-reflecting half mirror 4 504 be in 0, half-reflecting half mirror 5 505 be in 1,
Half-reflecting half mirror 1 is in 1, half-reflecting half mirror 2 502 and half-reflecting half mirror 4 504 close off Raman spectrum module and
The light output channel of near infrared spectrum module, avoids the attenuation by absorption of light disturbance and optical transmission process；
S4. near infrared light time spectrum is acquired, the controller in module 5 is regulated and controled by optics and automatically controls half-reflecting half mirror two
502 are in 1 in 1, half-reflecting half mirror 5 505 in 0, half-reflecting half mirror 4 504 in 0, half-reflecting half mirror 3 503
Position, half-reflecting half mirror 1 are in 0, and half-reflecting half mirror 2 502 and half-reflecting half mirror 3 503 close off Raman spectrum mould
The light output channel of block and fluorescence spectrum module, avoids the attenuation by absorption of light disturbance and optical transmission process；
S5. by parameter setting, optics regulates and controls module 5 and automatically controls successively acquisition micro Raman spectra, microscopic fluorescence light
Spectrum and micro- near infrared spectrum, and by incoming 507 acquired image of camera, computer 6 is transferred to by data line respectively and is used
In subsequent processing.
As a preferred technical solution of the present invention, the acquisition control software 7 is programmed using Visual Studio
It realizes, by the Software Development Kit for calling each spectrometer supplier to provide, realizes the information exchange with each spectrometer；It is described
Every control instruction of acquisition control Integrated Simulation optics regulation module 5 and light source switch；The acquisition control software is shown
Show interface can with real-time display acquire spectrogram, built-in form selection, spectrum baseline correction, Pretreated spectra, clustering,
The functions such as pattern-recognition and calibration model foundation；The acquisition control software development environment is 7 flagship of computer system Windows
Version (32), 2010 Ultimate of Visual Studio, the executable file of the acquisition control software of exploitation are described to hold
Style of writing part may be mounted at the computer or industrial personal computer for meeting minimalist configuration, have preferable portable and compatibility.
Embodiment 2:
By micro- multi-modal fusion spectral detection system exemplary application described in embodiment 1 in the fast high-sensitive of grain security
Detection, specifically with aflatoxin B in corn1For Testing index, Fig. 3 shows micro- multi-modal fusion spectral detection model and builds
Vertical process schematic, implementation steps are as follows:
L1. representative corn sample is collected, and by sample pulverization process, is drawn corn sample at random in the ratio of about 4:3
It is divided into calibration set and verifying collection, such as collects 56 parts of sample, divides 32 parts of calibration set sample, verifying collection 24；
L2. micro- multi-modal fusion spectral detection system of the present invention is used, sets collecting flowchart on software, respectively
Homologous Raman spectrum, fluorescence spectrum and the near infrared spectrum for obtaining same sample position point；
L3. principal component analysis, isolated component point are selected to Raman spectrum, fluorescence spectrum and the near infrared spectrum that L2 is obtained
One of analysis, wavelet analysis or combinations thereof mode carry out spectral signature variable extraction respectively, big with the accumulative contribution of characteristic variable
It is Variable Selection threshold value in 90%, the characteristic variable of three kinds of spectrum of screening constitutes fusion spectral signature matrix of variables；
L4. it is sampled after the spectrum of corn sample collection multiple modalities in same position, it is measured using standard method and is waited for
Index value is surveyed, as the reference value for establishing calibration equation；Here aflatoxin B in corn1Measurement refer to GB 5009.22-
2016 " measurements of aflatoxin B race and G race in national food safety standard food "；
L5. to fusion spectral signature matrix of variables obtained in L3, aflatoxin B in the corn measured in conjunction with L41's
Reference value, in the way of one kind or combinations thereof in principal component regression, Partial Least Squares, artificial neural network, support vector machines
Establish aflatoxin B in corn1Detection model, with verifying collection sample carry out model performance verifying.
Further, for verify the micro- multi-modal fusion spectral detection system compared to single spectrum detection technique and
The beneficial effect of heterologous Spectrum Data Fusion does both sides verification experimental verification:
On the one hand, compared to single spectrum detection technique, distinguished using the micro- multi-modal fusion spectral detection system
The lower three kinds of spectroscopic datas of the same microcell of corn sample are acquired, establish detection model and the fusion of multi-modal spectrum of single spectrum respectively
Detection model, model result such as table 1；Compared to single spectral detection as a result, using multi-modal fusion establishment of spectrum detection model
There is conspicuousness raising in terms of detection accuracy and sensitivity, in food safety traces component measure, daily cosmetics effective component
Detection etc. has Substantial technical advantage.
The micro- multi-modal fusion spectrum of table 1. is compared with single spectral detection result
On the other hand, right using the micro- multi-modal fusion spectral detection system compared to heterologous Spectrum Data Fusion
Same corn sample horizontal direction after the spectrum for acquiring a kind of mode arbitrarily adjusts the light that sampling location obtains another mode again
Spectrum, and so on obtain the spectrum of the third mode again, the entire sample is then measured its finger to be measured using standard method
Scale value；Because of the randomness of sample point, the multi-modal spectra collection of batch sample takes method parallel three times, compares detection effect,
As shown in table 2；The spectroscopic data that different sampling locations obtain, the heterolgous fusion spectral detection model of foundation, because adopting are obtained three times
The randomness (not guaranteeing homology) of sample, with the result poor repeatability of batch pattern detection, testing result is significantly worse than homologous
Multi-modal spectrum Fusion Model.
The micro- multi-modal fusion spectrum of table 2. is compared with heterologous multi-modal fusion spectral detection result
By verification result it can be found that the micro- multi-modal fusion spectral detection system, solves single spectral technique
The information mismatch problem for obtaining the not comprehensive and multispectral information fusion of information, guarantees the fining that multi-modal spectral information obtains
And homology, compared with prior art, in detectability and the precision aspect present invention with substantive distinguishing features outstanding and significantly
Progress.
Although the embodiments of the invention are described in conjunction with the attached drawings, but those skilled in the art can not depart from this hair
Various modifications and variations are made in the case where bright spirit and scope, such modifications and variations are each fallen within by appended claims
Within limited range.
Claims (9)
1. a kind of micro- multi-modal fusion spectral detection system, which is characterized in that the system comprises Raman spectrum module (1),
Fluorescence spectrum module (2), near infrared spectrum module (3), optical microphotograph module (4), optics regulation module (5), computer (6) and
Acquisition control software (7)；The optical microphotograph module (4) and Raman spectrum module (1), fluorescence spectrum module (2), near infrared light
Module (3) organic linking is composed, by optic path and control, for obtaining object to be measured automatically with a variety of under the micro-scale of position
The spectral information of mode；
The computer (6) passes through data line and Raman spectrum module (1), fluorescence spectrum module (2), near infrared spectrum module
(3), optical microphotograph module (4), optics regulation module (5) are connected；
The Raman spectrum module (1) is used to obtain the micro Raman spectra of object to be measured；
The fluorescence spectrum module (2) is used to obtain the micro-fluorescence spectrum of object to be measured；
The near infrared spectrum module (3) is used to obtain the micro- near infrared spectrum of object to be measured；
The optics regulates and controls module (5), is used for optical path modulation and control；Regulate and control module (5) by the optics, to each light
It composes light emitting source in module and issues light progress optical path modulation and channel closure, light enters optical microphotograph module (4) primary optical axis and realizes light
Transmission；
The acquisition control software (7) is installed in computer (6), for system parameter setting, interact with each module information, into
Row spectrum data gathering is shown to be handled with the analysis of data；
The near infrared spectrum module (3) includes tungsten halogen lamp (301), collimating mirror three (302), half-reflecting half mirror one (303), focuses
Mirror three (304), fiber coupler three (305) and near infrared spectrometer (306)；The collimated mirror three of light that tungsten halogen lamp (301) issues
(302) it is transferred in the primary optical axis of optical microphotograph module (4) after collimating, passes through object lens (501) after half-reflecting half mirror four (504)
It is transferred to sample surface, sample diffuses by object lens (501), half-reflecting half mirror four (504), through half-reflecting half mirror five
(505) after, a part of light is passed to camera, and for acquiring image, a part is divided by half-reflecting half mirror one (303) after reflection
Two-way, a part of line focus mirror three (304) enter fiber coupler three (305), obtain finally by near infrared spectrometer (306)
Micro- near infrared spectrum；Another way passes to eyepiece through reflecting mirror three (508), acquires and determines sampled point by eyepiece before spectrum.
2. a kind of micro- multi-modal fusion spectral detection system according to claim 1, which is characterized in that the Raman light
Composing module (1) includes laser (101), beam expander (102), collimating mirror one (103), laser line filter (104), reflecting mirror one
(105), dichroic mirror one (106), focus lamp one (107), fiber coupler one (108) and Raman spectrometer (109)；Laser
(101) light issued becomes collimated light beam by collimating mirror one (103) again after beam expander (102) expands, and filters through laser rays
Piece (104) filters out stray light, reflects light to dichroic mirror one through reflecting mirror one (105), then through dichroic mirror one (106) and half anti-half
Lens two (502) reflection is transferred to sample surface by object lens (501), and sample diffuses through object lens (501) to half anti-half
After lens two (502), for a part of light through passing to camera (507), a part of light is reflected into dichroic mirror one (106), line focus mirror
One (107) enter fiber coupler one (108), obtain micro Raman spectra finally by Raman spectrometer (109).
3. a kind of micro- multi-modal fusion spectral detection system according to claim 1, which is characterized in that the fluorescence light
Spectrum module (2) includes ultraviolet lamp (201), collimating mirror two (202), reflecting mirror two (203), dichroic mirror two (204), the logical optical filtering of long wave
Piece (205), focus lamp two (206), fiber coupler two (207) and Fluorescence Spectrometer (208)；The light that ultraviolet lamp (201) issues
Collimated mirror two (202) becomes collimated light beam, is reflected on dichroic mirror two through reflecting mirror two (203), then through dichroic mirror two (204)
It is reflected with half-reflecting half mirror three (503), is transferred to sample surface by object lens (501), sample diffuses through object lens (501)
To after half-reflecting half mirror three (503), for a part of light through camera (507) are passed to, a part of light is reflected into dichroic mirror two (204),
Exciting light and stray light are eliminated through long wave pass filter (205), then line focus mirror two (206) enters fiber coupler two
(207), micro-fluorescence spectrum is obtained finally by Fluorescence Spectrometer (208).
4. a kind of micro- multi-modal fusion spectral detection system according to claim 1, which is characterized in that the optics is aobvious
Micromodule (4) includes precision three-dimensional mobile platform (401), light box (402), object lens (501), half-reflecting half mirror two (502), half anti-
It is pellicle mirror three (503), half-reflecting half mirror four (504), half-reflecting half mirror five (505), focus lamp four (506), camera (507), anti-
Penetrate mirror three (508) and eyepiece (509)；The precision three-dimensional mobile platform (401) for fine adjustment sample region to be measured and
Position is placed in light box；The light box (402) is for shielding external stray light into the micro- multi-modal fusion spectral detection
System；The camera (507) is used for capturing sample image；When sample collection position is adjusted, is observed and acquired by eyepiece (509)
Sample areas morphologic localization feature, determine optimal spectra collection point.
5. a kind of micro- multi-modal fusion spectral detection system according to claim 4, which is characterized in that the optics is aobvious
Optical component object lens (501), half-reflecting half mirror two (502), half-reflecting half mirror three (503), half-reflecting half mirror in micromodule (4)
Four (504), half-reflecting half mirror five (505) center line be primary optical axis.
6. a kind of micro- multi-modal fusion spectral detection system according to claim 4, which is characterized in that the optics is aobvious
Half-reflecting half mirror two (502), half-reflecting half mirror three (503), half-reflecting half mirror four (504), half-reflecting half mirror in micromodule (4)
Five (505) are embedded on optical channel closure substrate；
Optics regulation module (5) transmits instruction control according to the parameter set in acquisition control software (7), by connection
It is closed by the optical channel of half-reflecting half mirror；
The closure substrate is the movable part of intermediate aperture, for assembling the half-reflecting half mirror, and with shutter execution unit
Substrate condition adjustment is realized in combination；The shutter execution unit be mechanical shutter, electronic shutter, combination Polarization Modulation device or
Spatial light modulation device.
7. a kind of micro- multi-modal fusion spectral detection system according to claim 6, which is characterized in that the closure base
Piece shares two states, first is that it is parallel with the primary optical axis of optical microphotograph module while vertical with spectrum acquisition module primary optical axis, i.e.,
0, this time can all pass through, second is that and spectrum parallel with the primary optical axis of optical microphotograph module acquisition module primary optical axis is in
45 °, i.e., 1, at this moment the half-reflecting half mirror is in working position.
8. a kind of micro- multi-modal fusion spectral detection system according to claim 1, which is characterized in that the computer
(6) by data line connection Raman spectrum module (1) in Raman spectrometer, the Fluorescence Spectrometer in fluorescence spectrum module (2),
Near infrared spectrometer near infrared spectrum module (3), by connection be separately connected camera in optical microphotograph module (4) and
Optics regulates and controls module (5).
9. a kind of micro- multi-modal spectral information acquisition methods for detecting Food Security Index, which is characterized in that the detection is adopted
It is completed with the described in any item micro- multi-modal fusion spectral detection systems of claim 1-8, wherein 0 time can be whole
Passing through, half-reflecting half mirror is in working position at 1, specifically operate in accordance with the following steps:
S1. the micro- multi-modal fusion spectral detection system carries out equipment initialization, and spectra collection parameter is then arranged, from
Eyepiece observation adjusts precision three-dimensional mobile platform to suitable sample collection position；
S2. Raman spectrum is acquired, is automatically controlled at half-reflecting half mirror two (502) by the controller in optics regulation module (5)
It is in 0, half-reflecting half mirror four (504) in 0, half-reflecting half mirror five (505) in 1, half-reflecting half mirror three (503)
1, half-reflecting half mirror one (303) be in 1, half-reflecting half mirror three (503) and half-reflecting half mirror four (504) close off fluorescence
The light output channel of spectroscopic module and near infrared spectrum module, avoids the attenuation by absorption of light disturbance and optical transmission process；
S3. fluorescence spectrum is acquired, is automatically controlled at half-reflecting half mirror two (502) by the controller in optics regulation module (5)
It is in 1, half-reflecting half mirror four (504) in 0, half-reflecting half mirror five (505) in 0, half-reflecting half mirror three (503)
1, half-reflecting half mirror one (303) be in 1, half-reflecting half mirror two (502) and half-reflecting half mirror four (504) close off Raman
The light output channel of spectroscopic module and near infrared spectrum module, avoids the attenuation by absorption of light disturbance and optical transmission process；
S4. near infrared spectrum is acquired, half-reflecting half mirror two (502) are automatically controlled by the controller in optics regulation module (5)
In 0, half-reflecting half mirror three (503) be in 0, half-reflecting half mirror four (504) be in 1, at half-reflecting half mirror five (505)
It is in 0 in 1, half-reflecting half mirror one (303), half-reflecting half mirror two (502) and half-reflecting half mirror three (503) close off drawing
The light output channel of graceful spectroscopic module and fluorescence spectrum module, avoids the attenuation by absorption of light disturbance and optical transmission process；
S5. by parameter setting, optics regulates and controls module (5) and automatically controls successively acquisition micro Raman spectra, micro-fluorescence spectrum
With micro- near infrared spectrum；Light is passed to camera (507) and collects image；The spectrum collected and image are passed through into number respectively
Computer (6) are transferred to according to line, are analyzed by the processing that acquisition control software (7) carry out data.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710082623.1A CN107044959B (en) | 2017-02-16 | 2017-02-16 | Micro- multi-modal fusion spectral detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710082623.1A CN107044959B (en) | 2017-02-16 | 2017-02-16 | Micro- multi-modal fusion spectral detection system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107044959A CN107044959A (en) | 2017-08-15 |
| CN107044959B true CN107044959B (en) | 2019-06-28 |
Family
ID=59544496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710082623.1A Active CN107044959B (en) | 2017-02-16 | 2017-02-16 | Micro- multi-modal fusion spectral detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107044959B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108007906B (en) * | 2017-11-30 | 2020-08-04 | 百色学院 | Long afterglow material phosphorescence excitation spectrum measurement system and method |
| CN108152290A (en) * | 2017-12-24 | 2018-06-12 | 大连益盛达智能科技有限公司 | A kind of display module detection of particles camera mechanism |
| CN110082335B (en) * | 2018-01-25 | 2022-07-26 | 齐鲁工业大学 | Infrared, Raman and laser three-channel point-to-point real-time fusion measuring system |
| CN110044838B (en) * | 2019-05-09 | 2021-06-25 | 青岛大学附属医院 | Optical detector for secretion |
| CN110579470A (en) * | 2019-09-13 | 2019-12-17 | 中国科学院新疆理化技术研究所 | method for detecting explosives through real-time in-situ characterization of multimode coupling optical platform |
| CN110441235A (en) * | 2019-09-13 | 2019-11-12 | 中国科学院新疆理化技术研究所 | A kind of Multiple modes coupling original position microspectrum imaging system |
| CN112285090A (en) * | 2020-09-29 | 2021-01-29 | 军事科学院系统工程研究院卫勤保障技术研究所 | Portable confocal unicellular Raman scattering detecting system |
| CN113834803B (en) * | 2021-09-22 | 2023-03-17 | 福州大学 | Multifunctional microscopic imaging optical system |
| CN114001643B (en) * | 2021-09-27 | 2023-07-25 | 上海工程技术大学 | Digital holographic microscopic phase distortion compensation method and device |
| CN118730953A (en) * | 2024-08-30 | 2024-10-01 | 陕西德丞电子科技有限公司 | Food safety detection system based on spectral analysis |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5528368A (en) * | 1992-03-06 | 1996-06-18 | The United States Of America As Represented By The Department Of Health And Human Services | Spectroscopic imaging device employing imaging quality spectral filters |
| CN1267713C (en) * | 2001-12-05 | 2006-08-02 | 财团法人工业技术研究院 | Spectral measuring apparatus for infrared spectrum, Raman spectrum and fluorescence spectrum |
| JP2003294618A (en) * | 2002-04-03 | 2003-10-15 | Jasco Corp | Infrared microspectroscopic apparatus and near-field infrared microspectroscopic apparatus |
| CN103743718B (en) * | 2013-12-11 | 2015-12-23 | 中国科学院西安光学精密机械研究所 | Laser spectrum analyzer combining confocal micro-Raman and laser-induced breakdown spectroscopy |
| CN103822908A (en) * | 2014-02-27 | 2014-05-28 | 江苏大学 | Fluorescence, Raman and laser induced atomic emission spectroscopy combined system |
| CN203811536U (en) * | 2014-04-24 | 2014-09-03 | 江西农业大学 | Multi-source information fusion-based rapid hogwash oil detection device |
-
2017
- 2017-02-16 CN CN201710082623.1A patent/CN107044959B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107044959A (en) | 2017-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107044959B (en) | Micro- multi-modal fusion spectral detection system | |
| Bergsträsser et al. | HyperART: non-invasive quantification of leaf traits using hyperspectral absorption-reflectance-transmittance imaging | |
| CN105190261B (en) | The spectroscopic methodology of marine product characterizes | |
| CN104284626B (en) | Fish-eye lens analyzer | |
| CN106662535B (en) | Determine the equipment, system and method for the optical properties of jewel | |
| CN102967604B (en) | Reflectance spectrum measuring and sampling system and method used for jewel detection | |
| US7495762B2 (en) | High-density channels detecting device | |
| CN110494966A (en) | For with the system and method for the metering of layer specific illumination spectrum | |
| CN107084790A (en) | Portable spectrometer and its spectral method of detection based on smart mobile phone | |
| CN108332855A (en) | A kind of hyperspectral imager device of Wavelength tunable | |
| CN112634212A (en) | Hyperspectral unmanned aerial vehicle-based disease latent tree detection method and system | |
| CN104730004B (en) | The discrimination method of the textile fabric based on UV Diffuse Reflectance Spectroscopy | |
| CN102565023A (en) | Device and method for fruit and vegetable pesticide residues based on laser Raman spectrometer | |
| CN101017083A (en) | High-density multi-channel testing device | |
| CN209085766U (en) | A kind of Spectral radiance measurement device | |
| CN104677299A (en) | Film detection device and method | |
| CN108169207A (en) | Space autofocusing laser differential confocal Raman spectrum imaging detection method and device | |
| CN102175638A (en) | Device for rapidly and nondestructively detecting component content of yellow rice wine | |
| CN107063456A (en) | Time resolution diffraction efficiency of grating spectral measurement device in situ and method | |
| CN110320197A (en) | Microminiature Raman blood specialized analyzer based on Raman spectrum analysis | |
| CN108627495A (en) | The Raman scattering Quick Acquisition and imaging device of fixed wave length | |
| CN101614663A (en) | As seen and near infrared spectrum bee pollen variety discriminating | |
| Yao et al. | Design and testing of an active light source apparatus for crop growth monitoring and diagnosis | |
| CN206248212U (en) | A kind of light source module and the line scanning multi-optical spectrum imaging system using it | |
| CN205982061U (en) | Clear measuring device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |