CN107043700B - Control method of magnetic control system for gene detection - Google Patents
Control method of magnetic control system for gene detection Download PDFInfo
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- CN107043700B CN107043700B CN201710428548.XA CN201710428548A CN107043700B CN 107043700 B CN107043700 B CN 107043700B CN 201710428548 A CN201710428548 A CN 201710428548A CN 107043700 B CN107043700 B CN 107043700B
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- 238000001514 detection method Methods 0.000 title claims abstract description 40
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims description 29
- 238000002156 mixing Methods 0.000 claims abstract description 34
- 229910000976 Electrical steel Inorganic materials 0.000 claims description 4
- 238000005336 cracking Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 208000026350 Inborn Genetic disease Diseases 0.000 description 4
- 208000016361 genetic disease Diseases 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 239000011324 bead Substances 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 210000002200 mouth mucosa Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a magnetic control system for gene detection, which comprises a mixing array (101) and a dragging array (102) which are arranged towards the same electromagnetic coil; the electromagnetic coils (1) in the mixing array (101) are annularly arranged; electromagnetic coils (1) in the dragging array (102) are arranged in a straight line, and the electromagnetic coils at the top end of the dragging array (102) are connected with the mixing array (101). The invention is a magnetic control system with convenient control, and can be used for integrated detection of genes.
Description
Technical Field
The invention relates to a control method of a magnetic control system for gene detection, and belongs to the technical field of medical detection.
Background
The gene detection is a technology for detecting DNA through blood, other body fluids or cells, namely oral mucosa cells or other tissue cells which are shed by a detected person are taken, after gene information of the oral mucosa cells or other tissue cells is amplified, DNA molecular information in the cells of the detected person is detected through specific equipment, risks of diseases of the body are predicted, and various genetic conditions contained in the oral mucosa cells or other tissue cells are analyzed, so that people can know own genetic information, and the occurrence of the diseases is avoided or delayed through improving living environments and living habits of the people.
Gene detection can be used for diagnosing diseases and predicting the risk of the diseases. Disease diagnosis is the detection of mutated genes that cause genetic disease using gene detection techniques. The most widely used genetic tests at present are the detection of genetic diseases of newborns, diagnosis of genetic diseases and auxiliary diagnosis of certain common diseases. Currently, more than 1000 genetic diseases can be diagnosed by gene detection techniques.
Since the sample usually used in gene detection is a biological tissue cell, the detection of the gene requires steps such as lysing, washing, amplifying, etc. of the cell, and then the optical detection is used to detect the gene. Because different treatments are involved in each step, the sample is usually required to be treated by a plurality of devices to be treated in different steps, detection is troublesome, and when the different steps are carried out, the sample is sometimes required to be transferred to different carriers, so that pollution is easily introduced in the process, and the detection precision is affected. Therefore, the steps of gene detection can be completed in the same kit, and the main development direction of the current research is that. Meanwhile, a device capable of performing integrated treatment on the kit will also have corresponding requirements, and an important point of research of the device is how to move the sample into different detection steps without contacting the sample.
Disclosure of Invention
The invention aims to provide a magnetic control system for gene detection. The magnetic control system is convenient to control and can be used for integrated detection of genes.
The technical scheme of the invention is as follows: the control method of the magnetic control system for gene detection is characterized in that the magnetic control system comprises a mixing array and a dragging array which are arranged towards the same electromagnetic coil; the electromagnetic coils in the uniformly mixed array are annularly arranged; the electromagnetic coils in the dragging array are arranged in a straight line, and the electromagnetic coils at the top end of the dragging array are connected with the uniformly mixed array.
In the above control method of a magnetic control system for gene detection, preferably, the electromagnetic coils of the drag array are divided into two left and right columns, wherein any one of the electromagnetic coils of one column is opposite to between two adjacent electromagnetic coils of the other column.
In the control method of the magnetic control system for gene detection, each electromagnetic coil in the uniformly mixed array sequentially completes the on-off process along the clockwise or anticlockwise direction and circulates for a plurality of times.
In the control method of the magnetic control system for gene detection, after the mixing array finishes working, each electromagnetic coil in the dragging array sequentially completes the on-off process from one end close to the mixing array to the other end.
In the foregoing control method of a magnetic control system for gene detection, after the blending array completes the work, each electromagnetic coil in the dragging array sequentially completes the on-off process from one end close to the blending array to the other end in a manner of two adjacent groups.
In the control method in the magnetic control system for gene detection, after the mixing array completes the work, each electromagnetic coil in the dragging array sequentially completes the on-off process in a mode of two or three adjacent groups from one end close to the mixing array to the other end; wherein the electromagnetic coils of each group comprise one electromagnetic coil in one of the columns and one or two electromagnetic coils closest thereto in the other column.
In the control method of the magnetic control system for gene detection, in the mixing array, the magnetic core of the electromagnetic coil is formed by tightly arranging 3 silicon steel sheets with the diameter of 0.65mm by 4mm by 60mm, and 6 layers of enameled wires (about 1200 bundle coils) with the wire diameter of 0.25mm are wound outside the magnetic core; the dragging array magnetic core is formed by tightly arranging 3 silicon steel sheets with the thickness of 0.65mm and 6mm and 60 mm; 6 layers of enameled wires (about 1200 coils) with the wire diameter of 0.25mm are wound on the outer surface of the magnetic core.
The 15V electric Gaussian meter is used for measuring the magnetic field intensity of the mixed array to be about 2000Gs, and the array is dragged to be about 2200Gs. The dragging effect is best when the electromagnetic coil is 1.3mm away from the magnetic bead, and the overheat condition of the electromagnetic coil can not occur.
In the control method of the magnetic control system for gene detection, the on-off interval of the electromagnetic coils in the uniformly mixed array is adjustable and controlled to be 15ms to 500ms.
In the control method of the magnetic control system for gene detection, the on-off interval of the electromagnetic coils in the mixing array is controlled to be 35 ms-65 ms, and the mixing effect is optimal.
Compared with the prior art, the invention utilizes the arrangement of the electromagnetic coils to form a whole array capable of generating a dynamic magnetic field, and further can utilize the high-speed circulating movement and the low-speed dragging of the magnetic carrier, when the magnetic carrier is used for a gene detection kit, the high-speed circulating movement of the magnetic carrier can be utilized, so that the sample is fully and uniformly mixed and cracked in the cracking liquid, and then the magnetic carrier is utilized to drive the sample to enter different areas for respectively carrying out multi-process treatment by utilizing the low-speed dragging, thereby enabling the gene integrated detection to be possible.
In addition, the invention utilizes the arrangement mode of the electromagnetic coils and the control of on-off of the electromagnetic coils to efficiently mix and move the magnetic carrier, so as to achieve the best cracking effect, and simultaneously can slowly drive the magnetic carrier to move in the kit, so that the magnetic carrier can smoothly pass through a narrow pore and is not easy to block.
Drawings
FIG. 1 is a schematic diagram of a single row electromagnetic coil array of the present invention;
fig. 2 is a schematic diagram of a double-row electromagnetic coil array structure according to the present invention.
The marks in the drawings are: 1-electromagnetic coil, 101-uniformly mixed array and 102-dragging array.
Detailed Description
The invention is further illustrated by the following figures and examples, which are not intended to be limiting.
Example 1. A magnetic control system for gene detection is shown in FIG. 1, and comprises a mixing array 101 and a dragging array 102 which are arranged towards the same electromagnetic coil; the electromagnetic coils 1 in the mixing array 101 are annularly arranged; the electromagnetic coils 1 in the dragging array 102 are arranged in a straight line, and the electromagnetic coils at the top end of the dragging array 102 are connected with the mixing array 101.
Each electromagnetic coil 1 in the mixing array 101 completes the on-off process in turn along the clockwise or anticlockwise direction, and circulates for a plurality of times. Or each electromagnetic coil 1 in the mixing array 101 sequentially completes the on-off process according to the shape of the 8, and circulates for a plurality of times, so that the mixing effect is better, and the test shows that the cracking efficiency is higher when the electromagnetic coil is used for sample cracking.
After the uniformly mixed array 101 finishes working, each electromagnetic coil 1 in the dragging array 102 can be operated according to a single on-off mode, such as A starting; then A stops, B starts; then B is stopped, C is started; then C stops, and so on.
Or in a group control mode of 2 bits, such as AB is started simultaneously, then AB is stopped, and BC is started; and so on. Or starting the device A for a period of time and then starting the device B, and stopping the device A after the device AB is simultaneously connected for a period of time; b is independently connected, C is started after a period of time, BC is simultaneously connected for a period of time, B is disconnected, and in this way, experiments prove that the mode is smoother when dragging the nano magnetic beads, and the success rate of dragging the magnetic beads exceeds 95%.
Example 2. A magnetic control system for gene detection is shown in FIG. 2, and comprises a mixing array 101 and a dragging array 102 which are arranged towards the same electromagnetic coil; the electromagnetic coils 1 in the mixing array 101 are annularly arranged; the electromagnetic coils 1 in the dragging array 102 are arranged in a straight line, and the electromagnetic coils at the top end of the dragging array 102 are connected with the mixing array 101. The electromagnetic coils 1 of the drag array 102 are divided into left and right columns, wherein any one electromagnetic coil 1 of one column is opposite to the space between two adjacent electromagnetic coils 1 of the other column.
Each electromagnetic coil 1 in the mixing array 101 completes the on-off process in turn along the clockwise or anticlockwise direction, and circulates for a plurality of times. Or each electromagnetic coil 1 in the mixing array 101 sequentially completes the on-off process according to the shape of the 8, and circulates for a plurality of times, so that the mixing effect is better, and the test shows that the cracking efficiency is higher when the electromagnetic coil is used for sample cracking.
After the uniformly mixed array 101 finishes working, each electromagnetic coil 1 in the dragging array 102 can be started in a mode of controlling one group of 2 bits, for example, AB is started at the same time, then AB is stopped, and BC is started; and so on.
It is also possible to start up simultaneously in a controlled manner of a group of 3 bits, such as ABC; then ABC stops and BCD starts; BCD then stops, CDE starts, and so on.
When the invention is used, the invention is applied to the integrated detection equipment of the integrated kit, samples in the kit can be uniformly mixed in a magnetic control mode, and the samples in the kit are driven to enter different areas of the kit to carry out different detection procedures.
Claims (4)
1. A control method of a magnetic control system for gene detection is characterized in that the magnetic control system comprises a mixing array (101) and a dragging array (102) which are arranged towards the same electromagnetic coil; the electromagnetic coils (1) in the mixing array (101) are annularly arranged; the electromagnetic coils (1) in the dragging array (102) are arranged in a straight line, and the electromagnetic coils at the top end of the dragging array (102) are connected with the uniformly-mixed array (101); when the mixing operation is carried out, each electromagnetic coil (1) in the mixing array (101) sequentially completes the on-off process along the clockwise or anticlockwise direction and circulates for a plurality of times; the electromagnetic coils (1) of the dragging array (102) are divided into left and right columns, wherein any one electromagnetic coil (1) of one column is opposite to the space between two adjacent electromagnetic coils (1) of the other column; after the mixing array (101) finishes working, each electromagnetic coil (1) in the dragging array (102) sequentially finishes the on-off process in a mode of two or three adjacent groups from one end close to the mixing array (101) to the other end, so as to realize dragging operation; wherein each set of electromagnetic coils (1) comprises one of the electromagnetic coils (1) in one column and one or two of the electromagnetic coils (1) closest thereto in the other column.
2. The method for controlling a magnetic control system for gene detection according to claim 1, wherein in the mixing array (102), a magnetic core of the electromagnetic coil (1) is formed by tightly arranging 3 silicon steel sheets with the diameter of 0.65mm by 4mm by 60mm, and 6 layers of enameled wires with the diameter of 0.25mm are wound outside the magnetic core; the magnetic core of the dragging array (102) is formed by tightly arranging 3 silicon steel sheets with the thickness of 0.65mm and 6mm and 60 mm; 6 layers of enameled wires with the wire diameter of 0.25mm are wound outside the magnetic core.
3. The control method of the magnetic control system for gene detection according to claim 2, wherein the on-off interval of the electromagnetic coils in the mixing array (101) is adjustable and controlled to be 15ms to 500ms.
4. The method of controlling a magnetic control system for gene detection according to claim 3, wherein the on-off intervals of the electromagnetic coils in the mixing array (101) are controlled to be 35ms to 65ms.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710428548.XA CN107043700B (en) | 2017-06-08 | 2017-06-08 | Control method of magnetic control system for gene detection |
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| CN201710428548.XA CN107043700B (en) | 2017-06-08 | 2017-06-08 | Control method of magnetic control system for gene detection |
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| CN107043700A CN107043700A (en) | 2017-08-15 |
| CN107043700B true CN107043700B (en) | 2023-11-24 |
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