CN107028967B - Application of the Saponin A in preparation treatment lung-cancer medicament - Google Patents
Application of the Saponin A in preparation treatment lung-cancer medicament Download PDFInfo
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- CN107028967B CN107028967B CN201710405761.9A CN201710405761A CN107028967B CN 107028967 B CN107028967 B CN 107028967B CN 201710405761 A CN201710405761 A CN 201710405761A CN 107028967 B CN107028967 B CN 107028967B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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Abstract
The present invention relates to application of the Saponin A in preparation treatment lung-cancer medicament.Saponin A in application of the present invention can promote aging and the cell-cycle arrest of lung carcinoma cell, inhibit the occurrence and development of lung cancer, have good inhibiting effect to the growth of lung carcinoma cell and animal-transplanted tumor.
Description
Invention field:
The present invention relates to the pharmaceutical products of the compound containing heterocycle, and in particular to comes from Rhamnaceae jujube saponin(e
The pharmaceutical preparation of constituents.
Background technique:
Lung cancer is the common malignant tumour in the whole world, and morbidity and mortality occupy first of all kinds of malignant tumours, it has also become
Seriously endanger one of human health and the disease of life.In recent years, due to being influenced by various factors, world community lung cancer morbidity
Rate and the death rate all obviously rise, especially Chinese, be the country that patients with lung cancer is most in the world and patients with lung cancer every year all at
Increasing trend.Therefore, the drug for finding efficient, low toxicity a treatment lung cancer, which seems, to be even more important, and in recent years, Chinese herbal medicine is with it
Unique glamour is increasingly taken seriously, and aggregation of the China as Chinese herbal medicine, has advantageous advantage, from Chinese herbal medicine
The anti-tumor drug of middle searching one efficiently, less toxic is a kind of means being particularly suitable for.
Semen ziziphi spinosae (Ziziphus jujuba Mill.var.spinosa) is being dried to for Rhamnaceae jujube wild jujube
Ripe kernel is distributed in northwest, northeast, many provinces and regions in North China and southern some areas more.Semen ziziphi spinosae contains a variety of active ingredients
And there is pharmacological activity abundant, such as alkaloid, saponin(e and flavones, there are the pharmacology such as calmness, hypnosis, analgesia, anticonvulsion
Effect.Semen ziziphi spinosae has tonifying liver, calming heart, arrest sweating, the effect of promoting the production of body fluid, and is mainly used for treating restlessness of asrhenia type and insomnia, horrified to much dream is physically weak more
The diseases such as sweat and injury thirst.Jujuboside is that triterpene compound is the valuable pharmacological in semen ziziphi spinosae with sedative-hypnotic effect
Active constituent is primarily present in its cotyledon.
Saponin A is a kind of triterpene compound that separation is extracted from semen ziziphi spinosae, and previous research finds it
Pharmacological action with tranquilizing soporific has had not yet to see the relevant report of its antitumor cell.
Summary of the invention:
The technical problem to be solved in the present invention is to provide the new applications of Saponin A, i.e., the new opplication in pharmacy.
The above-mentioned new opplication in pharmacy is application of the Saponin A in preparation treatment lung-cancer medicament.
In above-mentioned application, the drug is made of Saponin A and medically acceptable auxiliary material.
In above-mentioned application, the drug is granule, effervescent tablet or soft capsule.
Saponin A in application of the present invention can promote aging and the cell-cycle arrest of lung carcinoma cell, inhibit
The occurrence and development of lung cancer have good inhibiting effect to the growth of lung carcinoma cell and animal-transplanted tumor.
Detailed description of the invention:
The microphoto of Fig. 1 various concentration Saponin A promotion lung carcinoma cell aging effects.
Effect picture of Fig. 2 various concentration Saponin A to lung carcinoma cell Cycle Arrest situation, wherein semen ziziphi spinosae in a figure
The concentration of saponin A is that the concentration of Saponin A in 0, b figure is 25 μM (micromoles per liters), the concentration of Saponin A in c figure
It is 50 μM, the concentration of Saponin A is 100 μM in d figure；E is the bar chart that various concentration compares.
Fig. 3 Saponin A inhibits the effect picture of the occurrence and development of lung cancer.
Specific embodiment
Experimental example 1: Saponin A promotes lung carcinoma cell aging
Logarithmic growth phase A549 cell, with 2 × 105/ hole is inoculated in six well culture plates, overnight incubation, discards culture
Base, PBS are cleaned 2 times, and appropriate 1x Fixative Solution is added in every hole, and fixed 10-15min discards fixer, uses PBS
Cleaning 2 times, every hole add 1ml to dye mixed liquor, 37 DEG C of overnight incubations (avoiding contact with carbon dioxide), microscopically observation.Such as Fig. 1
Shown, Saponin A can promote cell ageing.
Experimental example 2: influence situation of the flow cytomery Saponin A to the lung carcinoma cell period
Logarithmic growth phase A549 cell, with 2 × 105/ hole is inoculated in six well culture plates, overnight incubation, discards culture
Base is added Saponin A (0,25,50,100 μM), after processing cell 5 days, with pancreatin digestion, collects, centrifugation, clear with PBS
Wash 2 times, then with pre-cooling volume fraction be 75% ethanol solution suspension cell, sealing be placed in 4 degree of refrigerator overnights.Next day, centrifugation,
PBS is washed twice, and 500 μ lPI are added in every pipe, is mixed, and room temperature, which is protected from light, is incubated for 15min, with flow cytomery period, sample 1h
It is interior to have detected.As shown in Fig. 2, Cycle Arrest is in the G0/G1 phase.
Experimental example 3: influence of the Saponin A to the occurrence and development of lung cancer
Urethane induces lung cancer model: choosing the FVB female mice of 6 week old sizes, is divided into 4 groups, is injected intraperitoneally by weight
600mg/Kg urethane (aqueous solution) amounts to 15 weeks once a week.Start that wild jujube is administered when third week is injected intraperitoneally in urethane
Benevolence saponin A (aqueous solution stomach-filling), 5 μ g/g of low dose group, 10 μ g/g of middle dose group, 20 μ g/g of high dose are given by weight stomach-filling daily
Medicine, the physiological saline of control (control) group stomach-filling equivalent.A mouse weight is weighed weekly.By the 22nd week, mouse is put to death,
Lung tissue is taken, counts the Lung neoplasm number of every group of mouse under the microscope, by formula " number of tumors=tumour tubercle sum/group
The total mice of survival " calculates every group of average lung tumor number.As a result as shown in figure 3, Saponin A is able to suppress the generation of tumour
And development, there is internal anti-tumor activity.
Experimental example 4: the preparation example of pharmaceutical preparation
Example 1 (granule)
It weighs Saponin A 25g, PVP 25g, CMC-Na 20g, L-HPC 8g, CMS-Na 6g and suitable is added
65% ethyl alcohol prepares softwood, crosses sieve, and 60 DEG C are dried to obtain particle, after whole grain of being sieved, is packaged into using packaging facilities
Granula.
Example 2 (effervescent tablet)
Saponin A 25g is weighed, it is equal that starch 30g, dextrin 12g, sucrose 18g and the mixing of suitable 65% ethyl alcohol is added
After even, granulation is dried to obtain particle, and particle is divided into two parts, and a part of particle is added calcium bicarbonate 2g and mixes, and in addition one
Part is added citric acid 1.5g, tartaric acid 1.5g and mixes, and is pressed into effervescent tablet.
Example 3 (soft capsule)
Saponin A 15g is weighed, vegetable oil 4g, starch 25g, gelatin 10g, water 10g and glycerol 2g is added, with rotation
Pressure capsule machine is pressed into soft capsule.
Claims (2)
1. application of the Saponin A as sole active ingredient in preparation treatment lung-cancer medicament.
2. the application according to claim 1, which is characterized in that the drug is granule, effervescent tablet or soft capsule
Agent.
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| CN201710405761.9A CN107028967B (en) | 2017-06-02 | 2017-06-02 | Application of the Saponin A in preparation treatment lung-cancer medicament |
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| CN107028967B true CN107028967B (en) | 2019-10-01 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102670764A (en) * | 2012-05-07 | 2012-09-19 | 南京中医药大学 | Active parts of wild jujube as well as preparation method and application thereof |
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| KR101090313B1 (en) * | 2010-05-25 | 2011-12-07 | 한국생명공학연구원 | Composition comprising for preventing and treating hepatitis b comprising compound of chemical formula 1 and process for the preparation thereof |
| EP2727473A4 (en) * | 2011-06-28 | 2015-05-06 | Vivozon Inc | Combination of effective substances causing synergistic effects of multiple targeting and use thereof |
| KR101999698B1 (en) * | 2013-08-14 | 2019-07-12 | 주식회사 엘지생활건강 | Composition for skin cell regeneration, anti-wrinkle, antioxidant, anti-imflamation, and skin whitening |
| WO2015055456A1 (en) * | 2013-10-15 | 2015-04-23 | Unilever Plc | Frozen confection |
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| CN102670764A (en) * | 2012-05-07 | 2012-09-19 | 南京中医药大学 | Active parts of wild jujube as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 酸枣仁中三萜皂苷的分离和结构研究;王建忠等;《有机化学》;20080115;第28卷(第1期);参见第69-72页 * |
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