CN107028967A - Application of the Saponin A in treatment lung-cancer medicament is prepared - Google Patents
Application of the Saponin A in treatment lung-cancer medicament is prepared Download PDFInfo
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- CN107028967A CN107028967A CN201710405761.9A CN201710405761A CN107028967A CN 107028967 A CN107028967 A CN 107028967A CN 201710405761 A CN201710405761 A CN 201710405761A CN 107028967 A CN107028967 A CN 107028967A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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Abstract
The present invention relates to application of the Saponin A in treatment lung-cancer medicament is prepared.Saponin A in application of the present invention can promote aging and the cell-cycle arrest of lung carcinoma cell, suppress the occurrence and development of lung cancer, have good inhibitory action to the growth of lung carcinoma cell and animal-transplanted tumor.
Description
Invention field：
The present invention relates to the pharmaceutical product of the compound containing heterocycle, and in particular to from Rhamnaceae jujube saponin(e
The pharmaceutical preparation of constituents.
Background technology：
Lung cancer is the common malignant tumour in the whole world, and its morbidity and mortality is occupy first of all kinds of malignant tumours, it has also become
Seriously endanger one of human health and the disease of life.In recent years, due to being influenceed by various factors, world community lung cancer morbidity
Rate and the death rate all substantially rise, especially Chinese, be in the world the most country of patients with lung cancer and patients with lung cancer it is annual all into
Increasing trend.Therefore, find one efficiently, the medicine of the treatment lung cancer of low toxicity seem and be even more important that in recent years, Chinese herbal medicine is with it
Unique glamour is increasingly taken seriously, and there is advantageous advantage in China, from Chinese herbal medicine as the aggregation of Chinese herbal medicine
Middle searching one is efficient, the antineoplastic of low toxicity is a kind of means being particularly suitable for.
Spina date seed (Ziziphus jujuba Mill.var.spinosa) is dried to for Rhamnaceae jujube wild jujube
Ripe benevolence, is distributed in northwest, northeast, many provinces and regions in North China and some southern areas more.Spina date seed contains various active composition
And with abundant pharmacological activity, such as alkaloid, saponin(e and flavones, with the pharmacology such as calmness, hypnosis, analgesia, anticonvulsion
Effect.Spina date seed has tonifying liver, calming heart, arrest sweating, the effect of promote the production of body fluid, is mainly used in treating restlessness of asrhenia type and insomnia, horrified to much dream is physically weak more
The disease such as sweat and injury thirst.Jujuboside is that triterpene compound is the valuable pharmacological with sedative-hypnotic effect in spina date seed
Active component, is primarily present in its cotyledon.
Saponin A is a kind of triterpene compound for extracting separation from spina date seed and coming, and research in the past finds it
Pharmacological action with tranquilizing soporific, has not yet to see the related report of its antitumor cell.
The content of the invention：
The technical problem to be solved in the present invention is to provide the new application of Saponin A, i.e., the new opplication in pharmacy.
The above-mentioned new opplication in pharmacy is that Saponin A is preparing the application in treating lung-cancer medicament.
In above-mentioned application, described medicine is made up of Saponin A and medically acceptable auxiliary material.
In above-mentioned application, described medicine is granule, effervescent tablet or soft capsule.
Saponin A in application of the present invention can promote aging and the cell-cycle arrest of lung carcinoma cell, suppress
The occurrence and development of lung cancer, have good inhibitory action to the growth of lung carcinoma cell and animal-transplanted tumor.
Brief description of the drawings：
Fig. 1 various concentrations Saponin A promotes the microphoto of lung carcinoma cell aging effects.
Fig. 2 various concentrations Saponin A to the design sketch of lung carcinoma cell Cycle Arrest situation, wherein, spina date seed in a figures
The concentration of saponin A is that the concentration of Saponin A in 0, b figures is the concentration of Saponin A in 25 μM (micromoles per liter), c figures
For 50 μM, the concentration of Saponin A is 100 μM in d figures；E is the bar chart that various concentrations compare.
Fig. 3 Saponin As suppress the design sketch of the occurrence and development of lung cancer.
Embodiment
Experimental example 1：Saponin A promotes lung carcinoma cell aging
Take the logarithm growth period A549 cell, with 2 × 105/ hole is inoculated in six well culture plates, overnight incubation, discards culture
Base, PBS 2 times adds appropriate 1x Fixative Solution, fixed 10-15min per hole, discards fixer, use PBS
Cleaning 2 times, adds 1ml to dye mixed liquor per hole, and 37 DEG C are incubated overnight (avoiding contact with carbon dioxide), micro- Microscopic observation.Such as Fig. 1
Shown, Saponin A can promote cell ageing.
Experimental example 2：Influence situation of the flow cytomery Saponin A to the lung carcinoma cell cycle
Take the logarithm growth period A549 cell, with 2 × 105/ hole is inoculated in six well culture plates, overnight incubation, discards culture
Base, add Saponin A (0,25,50,100 μM), processing cell was digested with pancreatin, collected, centrifuged after 5 days, clear with PBS
Wash 2 times, then with the volume fraction of precooling be 75% ethanol solution suspension cell, sealing is placed in 4 degree of refrigerator overnights.Next day, centrifugation,
PBS is washed twice, and often pipe adds 500 μ lPI, is mixed, and room temperature lucifuge is incubated 15min, uses flow cytomery cycle, sample 1h
It is interior to have detected.As shown in Fig. 2 Cycle Arrest is in the G0/G1 phases.
Experimental example 3：Influence of the Saponin A to the occurrence and development of lung cancer
Urethane induces lung cancer model：The FVB female mices of 6 week old sizes are chosen, is divided into 4 groups, is injected intraperitoneally by body weight
600mg/Kg urethanes (aqueous solution), once in a week, 15 weeks altogether.Start that wild jujube is administered when urethane is injected intraperitoneally the 3rd week
Benevolence saponin A (aqueous solution gavage), the μ g/g of low dose group 5, the μ g/g of middle dose group 10, the μ g/g of high dose 20 are given by body weight gavage daily
The physiological saline of gavage equivalent is organized in medicine, control (control).A mouse weight is weighed weekly.By the 22nd week, mouse is put to death,
Lung tissue is taken, the Lung neoplasm number of every group of mouse is counted under the microscope, by formula " tubercle sum/group of number of tumors=tumour
The total mice of survival " calculates every group of average lung knurl number.As a result as shown in figure 3, Saponin A can suppress the generation of tumour
And development, with internal antitumor activity.
Experimental example 4：The preparation example of pharmaceutical preparation
Example 1 (granule)
Weigh Saponin A 25g, add PVP 25g, CMC-Na 20g, L-HPC 8g, CMS-Na 6g and appropriate
65% ethanol prepares softwood, crosses screen cloth, and 60 DEG C are dried to obtain after particle, sieving whole grain, is packaged into using packaging facilities
Granula.
Example 2 (effervescent tablet)
Saponin A 25g is weighed, starch 30g, dextrin 12g, sucrose 18g and appropriate 65% ethanol mixing is added equal
After even, granulation is dried to obtain particle, and particle is divided into two parts, and a part of particle adds calcium bicarbonate 2g and mixed, and in addition one
Part adds citric acid 1.5g, tartaric acid 1.5g and mixed, and is pressed into effervescent tablet.
Example 3 (soft capsule)
Saponin A 15g is weighed, vegetable oil 4g, starch 25g, gelatin 10g, water 10g and glycerine 2g are added, with rotation
Pressure capsule machine is pressed into soft capsule.
Claims (2)
1. application of the Saponin A in treatment lung-cancer medicament is prepared.
2. application according to claim 1, it is characterised in that described medicine is granule, effervescent tablet or soft capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710405761.9A CN107028967B (en) | 2017-06-02 | 2017-06-02 | Application of the Saponin A in preparation treatment lung-cancer medicament |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710405761.9A CN107028967B (en) | 2017-06-02 | 2017-06-02 | Application of the Saponin A in preparation treatment lung-cancer medicament |
Publications (2)
| Publication Number | Publication Date |
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| CN107028967A true CN107028967A (en) | 2017-08-11 |
| CN107028967B CN107028967B (en) | 2019-10-01 |
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| CN201710405761.9A Active CN107028967B (en) | 2017-06-02 | 2017-06-02 | Application of the Saponin A in preparation treatment lung-cancer medicament |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115006421A (en) * | 2022-07-01 | 2022-09-06 | 上海健康医学院 | Application of jujuboside B in preparation of angiogenesis inhibiting medicine |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110129268A (en) * | 2010-05-25 | 2011-12-01 | 한국생명공학연구원 | Hepatitis V prevention or treatment composition comprising the compound of formula (1) as an active ingredient and a method for preparing the same |
| CN102670764A (en) * | 2012-05-07 | 2012-09-19 | 南京中医药大学 | Active parts of wild jujube as well as preparation method and application thereof |
| EP2727473A2 (en) * | 2011-06-28 | 2014-05-07 | Vivozon, Inc. | Combination of effective substances causing synergistic effects of multiple targeting and use thereof |
| KR20150019573A (en) * | 2013-08-14 | 2015-02-25 | 주식회사 엘지생활건강 | Composition for skin cell regeneration, anti-wrinkle, antioxidant, anti-imflamation, and skin whitening |
| WO2015055456A1 (en) * | 2013-10-15 | 2015-04-23 | Unilever Plc | Frozen confection |
-
2017
- 2017-06-02 CN CN201710405761.9A patent/CN107028967B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110129268A (en) * | 2010-05-25 | 2011-12-01 | 한국생명공학연구원 | Hepatitis V prevention or treatment composition comprising the compound of formula (1) as an active ingredient and a method for preparing the same |
| EP2727473A2 (en) * | 2011-06-28 | 2014-05-07 | Vivozon, Inc. | Combination of effective substances causing synergistic effects of multiple targeting and use thereof |
| CN102670764A (en) * | 2012-05-07 | 2012-09-19 | 南京中医药大学 | Active parts of wild jujube as well as preparation method and application thereof |
| KR20150019573A (en) * | 2013-08-14 | 2015-02-25 | 주식회사 엘지생활건강 | Composition for skin cell regeneration, anti-wrinkle, antioxidant, anti-imflamation, and skin whitening |
| WO2015055456A1 (en) * | 2013-10-15 | 2015-04-23 | Unilever Plc | Frozen confection |
Non-Patent Citations (2)
| Title |
|---|
| MEI-YING XU ET AL: "Antitumor activity of jujuboside B and the underlying mechanism via induction of apoptosis and autophagy", 《JOURNAL OF NATURAL PRODUCTS》 * |
| 王建忠等: "酸枣仁中三萜皂苷的分离和结构研究", 《有机化学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115006421A (en) * | 2022-07-01 | 2022-09-06 | 上海健康医学院 | Application of jujuboside B in preparation of angiogenesis inhibiting medicine |
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| CN107028967B (en) | 2019-10-01 |
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