CN107027808A - The antimicrobial plant vaccine and preparation method of energy control of plant bacterial wilt - Google Patents
The antimicrobial plant vaccine and preparation method of energy control of plant bacterial wilt Download PDFInfo
- Publication number
- CN107027808A CN107027808A CN201710251958.1A CN201710251958A CN107027808A CN 107027808 A CN107027808 A CN 107027808A CN 201710251958 A CN201710251958 A CN 201710251958A CN 107027808 A CN107027808 A CN 107027808A
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- Prior art keywords
- plant
- bacterial wilt
- vaccine
- antimicrobial
- energy control
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of antimicrobial plant vaccine of energy control of plant bacterial wilt and preparation method thereof, belongs to a kind of plant vaccine and preparation method.The vaccine includes Pi Shi Ralstoniasps, glycerine, MgSO4•7H2O、FeSO4•7H2O, distilled water.Its advantage is：Pi Shi Ralstonias bacterium is at war with and fought for nutrient and living space specifically designed for bacterial wilt germ, nutritional supplementation can only be carried out by changing given plant vaccine, situation about can not be suppressed for bacterial wilt, and it is simple controllable, it compensate for original vaccine and match complex defect.
Description
Technical field
The invention belongs to a kind of plant vaccine and preparation method, more particularly to antimicrobial plant vaccine and preparation method.
Technical background
This disease of bacterial wilt is a kind of bacterial disease that complete stool can be caused to wilt, and is infected and drawn by bacterium Pseudomonas solanacearum
Rise.In the market eliminates Pseudomonas solanacearum using chemicals, so as to reach the purpose for eliminating bacterial wilt.But chemicals pair
Environment is unfriendly, easily triggers pollution.
The content of the invention
To solve the above problems, the present invention provides a kind of antimicrobial plant vaccine of energy control of plant bacterial wilt, its is special
Nutrient and living space are at war with and fought for for bacterial wilt germ, and nutrition can only be carried out by changing given plant vaccine
Supplement, it is impossible to situation about being suppressed for bacterial wilt, and it is simple controllable, it compensate for original vaccine and match complex lack
Fall into.
To achieve the above object, a kind of technical scheme of the antimicrobial plant vaccine of energy control of plant bacterial wilt of the present invention
It is：
It is a kind of can control of plant bacterial wilt antimicrobial plant vaccine, it is characterized in that, include Pi Shi Ralstoniasps, sweet
Oil, MgSO4•7H2O、FeSO4•7H2O, distilled water.
Wherein, the pH value of the antimicrobial plant vaccine of described energy control of plant bacterial wilt is 7.
Wherein, the antimicrobial plant vaccine of described energy control of plant bacterial wilt can be made by the way of pouring root or injection
With.
The present invention it is a kind of can the beneficial effect of antimicrobial plant vaccine of control of plant bacterial wilt be：Pi Shi Rolls leads to
Salmonella is at war with and fought for nutrient and living space specifically designed for bacterial wilt germ, and changing given plant vaccine can only
Carry out nutritional supplementation, it is impossible to situation about being suppressed for bacterial wilt, and it is simple controllable, it compensate for original vaccine proportioning more
Complicated defect.
A kind of preparation method of the antimicrobial plant vaccine of energy control of plant bacterial wilt, it is characterized in that：
Step 1, glycerine, MgSO are prepared4•7H2O、FeSO4•7H2O, distilled water, its ratio are glycerine：MgSO4•7H2O：FeSO4•
7H2O：Distilled water=20ml：3g：3g：2000ml, obtains the first KB mixtures；
Step 2, pH value is adjusted, it is 7 to make pH value, obtains the 2nd KB mixtures；
Step 3, the 2nd KB mixtures are heated, keeping temperature is to be chemically reacted inside 121 degree, the 2nd KB mixtures, instead
Sterilized after the completion of answering, and be cooled to 31 degree, acquisition KB solution；
Step 4, by Pi Shi Ralstoniasps, stirring reaction in KB solution is inserted, when bacterium amount >=1 × 1010During strain/ml, instead
Should be abundant, obtain the antimicrobial plant vaccine of energy control of plant bacterial wilt.
Brief description of the drawings
Fig. 1 is the flow chart of the preparation method of the antimicrobial plant vaccine of energy control of plant bacterial wilt.
Embodiment
To achieve the above object, a kind of technical scheme of the antimicrobial plant vaccine of energy control of plant bacterial wilt of the present invention
It is：
It is a kind of can control of plant bacterial wilt antimicrobial plant vaccine, it is characterized in that, include Pi Shi Ralstoniasps, sweet
Oil, MgSO4•7H2O、FeSO4•7H2O, distilled water.
Wherein, the pH value of the antimicrobial plant vaccine of described energy control of plant bacterial wilt is 7.
Wherein, the antimicrobial plant vaccine of described energy control of plant bacterial wilt can be made by the way of pouring root or injection
With.
The preparation method of the antimicrobial plant vaccine of energy control of plant bacterial wilt is explained in detail with reference to Fig. 1.
A kind of preparation method of the antimicrobial plant vaccine of energy control of plant bacterial wilt, it is characterized in that,
Step 1, glycerine, MgSO are prepared4•7H2O、FeSO4•7H2O, distilled water, its ratio are glycerine：MgSO4•7H2O：FeSO4•
7H2O：Distilled water=20ml：3g：3g：2000ml, obtains the first KB mixtures；
Step 2, pH value is adjusted, it is 7 to make pH value, obtains the 2nd KB mixtures；
Step 3, the 2nd KB mixtures are heated, keeping temperature is to be chemically reacted inside 121 degree, the 2nd KB mixtures, instead
Sterilized after the completion of answering, and be cooled to 31 degree, acquisition KB solution；
Step 4, by Pi Shi Ralstoniasps, stirring reaction in KB solution is inserted, when bacterium amount >=1 × 1010During strain/ml, instead
Should be abundant, obtain the antimicrobial plant vaccine of energy control of plant bacterial wilt.
Claims (4)
1. it is a kind of can control of plant bacterial wilt antimicrobial plant vaccine, it is characterized in that, include Pi Shi Ralstoniasps,
Glycerine, MgSO4•7H2O、FeSO4•7H2O, distilled water.
2. the antimicrobial plant vaccine of energy control of plant bacterial wilt according to claim 1, it is characterized in that, tomato can be prevented and treated
The pH value of the antimicrobial plant vaccine of bacterial wilt is 7.
3. the antimicrobial plant vaccine of energy control of plant bacterial wilt according to claim 1, it is characterized in that, described energy is prevented
Controlling the antimicrobial plant vaccine of bacterial wilt of tomato can be used by the way of pouring root or injection.
4. a kind of preparation method of the antimicrobial plant vaccine of energy control of plant bacterial wilt, it is characterized in that,
Step 1, glycerine, MgSO are prepared4•7H2O、FeSO4•7H2O, distilled water, its ratio are glycerine：MgSO4•7H2O：FeSO4•
7H2O：Distilled water=20ml：3g：3g：2000ml, obtains the first KB mixtures；
Step 2, pH value is adjusted, it is 7 to make pH value, obtains the 2nd KB mixtures；
Step 3, the 2nd KB mixtures are heated, keeping temperature is to be chemically reacted inside 121 degree, the 2nd KB mixtures, instead
Sterilized after the completion of answering, and be cooled to 31 degree, acquisition KB solution；
Step 4, by Pi Shi Ralstoniasps, stirring reaction in KB solution is inserted, when bacterium amount >=1 × 1010During strain/ml, instead
Should be abundant, obtain the antimicrobial plant vaccine of energy control of plant bacterial wilt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710251958.1A CN107027808A (en) | 2017-04-18 | 2017-04-18 | The antimicrobial plant vaccine and preparation method of energy control of plant bacterial wilt |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710251958.1A CN107027808A (en) | 2017-04-18 | 2017-04-18 | The antimicrobial plant vaccine and preparation method of energy control of plant bacterial wilt |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107027808A true CN107027808A (en) | 2017-08-11 |
Family
ID=59534970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710251958.1A Pending CN107027808A (en) | 2017-04-18 | 2017-04-18 | The antimicrobial plant vaccine and preparation method of energy control of plant bacterial wilt |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107027808A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533616A (en) * | 2012-02-23 | 2012-07-04 | 东华大学 | Ralstonia pickettii highly resistant to cadmium and extraction method and application thereof |
| CN102978132A (en) * | 2012-11-19 | 2013-03-20 | 南京农业大学 | Microorganism plant vaccine capable of controlling tomato bacterial wilt disease |
-
2017
- 2017-04-18 CN CN201710251958.1A patent/CN107027808A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533616A (en) * | 2012-02-23 | 2012-07-04 | 东华大学 | Ralstonia pickettii highly resistant to cadmium and extraction method and application thereof |
| CN102978132A (en) * | 2012-11-19 | 2013-03-20 | 南京农业大学 | Microorganism plant vaccine capable of controlling tomato bacterial wilt disease |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170811 |