CN107022491A - Solution of the coccidian oocyst rate of recovery and preparation method thereof in a kind of raising chicken tissues - Google Patents
Solution of the coccidian oocyst rate of recovery and preparation method thereof in a kind of raising chicken tissues Download PDFInfo
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- CN107022491A CN107022491A CN201610065737.0A CN201610065737A CN107022491A CN 107022491 A CN107022491 A CN 107022491A CN 201610065737 A CN201610065737 A CN 201610065737A CN 107022491 A CN107022491 A CN 107022491A
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Abstract
Solution of the coccidian oocyst rate of recovery and preparation method thereof in chicken tissues is improved the present invention relates to a kind of, the solution component and its percentage by weight are：5-30 parts of aluminium polychloride；5-30 parts of tetrasodium ethylenediamine tetraacetate；0.1-0.5 parts of guar hydroxypropyltrimonium ammonium chloride；5-30 parts of sodium hypochlorite；10-90 parts of aqua sterilisa.Solution of the present invention is separated to the coccidian oocyst in tissue using effects such as flocculation, absorption, digestion, purified, the float assembly speed of the excrement egg capsule yields such as saturated saline floatation, sucrose solution floating method, saturation Adlerika floating method and egg capsule of comparing is increased substantially, the ratio of impurity is then effectively reduced simultaneously, isolate and purify soon, the sample infected available for high, weak two kinds of intensity is isolated and purified.
Description
Technical field
It is especially a kind of to improve solution of the coccidian oocyst rate of recovery and preparation method thereof in chicken tissues the invention belongs to veterinary biologics field
Background technology
Coccidiosis of domestic fowls is to endanger maximum spurious parasitosis under intensive, scale situation; gradually it is changed into both at home and abroad under the advocating of food security by drug therapy before disease and is prevented, the then research and development of coccidial vaccine turns into the new lover that coccidiosis of domestic fowls is resisted in livestock with production.The coccidian oocyst that current state intradermal vaccine is obtained is largely to be obtained using methods such as saturated saline floatation, sucrose solution floating method, saturation Adlerika floating method, chromatosulfuric acid separation from excrement, although it is simple and easy to apply, most of coccidian oocyst and impurity can be separated, but often mix impurity close with coccidian oocyst on a small quantity in the egg capsule separated, some egg capsules are difficult to separate by the crude fibre in some feeds, fat granule embedding.It is in caecum to poison eimeria tenella and Eimeria Tenella zoogamy, if it is too many that egg capsule impurity is collected from excrement, obtain egg capsule quantity very little, and obtained egg capsule is possible to be polluted by other egg capsules, therefore, according to coccidia Biological characteristics, a kind of method for improving acquisition coccidian oocyst in tissue is researched and developed imperative.
The content of the invention
Solution of the coccidian oocyst rate of recovery and preparation method thereof in chicken tissues is improved it is an object of the invention to provide a kind of, compare and collect the yield that egg capsule improves egg capsule in the excrement such as saturated saline floatation, sucrose solution floating method, saturation Adlerika floating method, improve the float assembly speed of egg capsule, reduce the ratio of impurity, isolate and purify fast, the present invention is isolated and purified available for the sample that high intensity infects, most probably in the sample of weak infection.
Coccidian oocyst rate of recovery and preparation method thereof in tissue is improved it is another object of the present invention to provide one kind.
The present invention is achieved by the following technical solutions：
Solution of the coccidian oocyst rate of recovery and preparation method thereof in a kind of raising chicken tissues, the component and its percentage by weight of its protection solution are：
Moreover, the protection solution component and its optimal ratio of weight and number are respectively：
It is the step of solution of the coccidian oocyst rate of recovery and preparation method thereof, its preparation method in a kind of raising chicken tissues：
(1) disodium ethylene diamine tetraacetate, aluminium polychloride, guar hydroxypropyltrimonium ammonium chloride, sodium hypochlorite is weighed successively according to weight proportion to be put into Agitation Tank, then aqua sterilisa is added to not having above-mentioned feed intake, after soaking 1 hour at room temperature, it is further continued for adding remaining aqua sterilisa, stir, it is completely dissolved to feeding intake, then solution A is obtained with 0.45um polyether sulfone filter element filterings, is stored into Agitation Tank；
(2) Agitation Tank heating is advisable with steam pressure 0.05Mpa, 15 minutes discharge condensed waters of evaporator once, every two hours discharge the condensation vapour of heater once.Indoor Natural is cooled down, standby, obtains solution B standby；
(3) with resulting solution B in 0.22um polyether sulfone filter element filtering steps (2), and solution pH value is adjusted to 6.0-7.0, stir 15 minutes, again with 0.22um polyether sulfone filter element filterings, obtain solution C, it is directly entered bottle placer by pipeline；
(4) resulting solution C in step (3) is filled in the medical vials after drying, you can obtain a kind of solution for improving the coccidian oocyst rate of recovery in chicken tissues.
Advantages of the present invention and beneficial effect are：
1. improving solution of the coccidian oocyst rate of recovery and preparation method thereof in chicken tissues it is an object of the invention to provide a kind of, inventive formulation composition is scientific and reasonable, and technique is simple, and easy to use, cost is relatively low.
The yield that egg capsule improves egg capsule is collected in the excrement such as saturated saline floatation, sucrose solution floating method, saturation Adlerika floating method 2. solution of the present invention compares, the float assembly speed of egg capsule is improved, the ratio of impurity is reduced, isolated and purified fast.
3. the present invention is isolated and purified available for the sample that high intensity infects, most probably in the sample of weak infection.
Embodiment
The present invention is described in further detail by following examples.It should be noted that：Following embodiments are illustrative, are not limited, it is impossible to limit protection scope of the present invention with following embodiments.
Aluminium polychloride, tetrasodium ethylenediamine tetraacetate in instant component；Guar hydroxypropyltrimonium ammonium chloride, sodium hypochlorite can be commercially commercially available.
Embodiment 1
The solution of the coccidian oocyst rate of recovery in a kind of raising chicken tissues, the component and its weight of its protection solution are respectively：Aluminium polychloride 5kg, tetrasodium ethylenediamine tetraacetate 5kg, guar hydroxypropyltrimonium ammonium chloride 0.1kg, sodium hypochlorite 5kg, aqua sterilisa 10kg.
It is the step of coccidian oocyst rate of recovery and preparation method thereof, its preparation method in one kind raising chicken tissues：
(1) disodium ethylene diamine tetraacetate, aluminium polychloride, guar hydroxypropyltrimonium ammonium chloride, sodium hypochlorite is weighed successively according to weight proportion to be put into Agitation Tank, then aqua sterilisa is added to not having above-mentioned feed intake, after soaking 1 hour at room temperature, it is further continued for adding remaining aqua sterilisa, stir, it is completely dissolved to feeding intake, then solution A is obtained with 0.45um polyether sulfone filter element filterings, is stored into Agitation Tank；
(2) Agitation Tank heating is advisable with steam pressure 0.05Mpa, 15 minutes discharge condensed waters of evaporator once, every two hours discharge the condensation vapour of heater once.Indoor Natural is cooled down, standby, obtains solution B standby；
(3) with resulting solution B in 0.22um polyether sulfone filter element filtering steps (2), and solution pH value is adjusted to 6.0-7.0, stir 15 minutes, again with 0.22um polyether sulfone filter element filterings, obtain solution C, it is directly entered bottle placer by pipeline；
(4) resulting solution C in step (3) is filled in the medical vials after drying, you can obtain a kind of solution for improving the coccidian oocyst rate of recovery in chicken tissues.
Embodiment 2
The solution of the coccidian oocyst rate of recovery in a kind of raising chicken tissues, the component and its weight of its protection solution are respectively：Aluminium polychloride 30kg, tetrasodium ethylenediamine tetraacetate 30kg, guar hydroxypropyltrimonium ammonium chloride 0.5kg, sodium hypochlorite 30kg, aqua sterilisa 90kg.
The step be the same as Example 1 of its preparation method.
Embodiment 3
The solution of the coccidian oocyst rate of recovery in a kind of raising chicken tissues, the component and its weight of its protection solution are respectively：Aluminium polychloride 15kg, tetrasodium ethylenediamine tetraacetate 15kg, guar hydroxypropyltrimonium ammonium chloride 0.3kg, sodium hypochlorite 15kg, aqua sterilisa 60kg.
The step be the same as Example 1 of its preparation method.
Clinical practice
Test explanation：
Murder by poisoning eimeria tenella Sporulated Oocysts, Eimeria Tenella Sporulated Oocysts that laboratory is preserved are taken, SPF15 age in days chicken inoculations, every 100,000, each each inoculation 20, totally four groups of every group of worm kind is given.Raised with aqua sterilisa and nonreactive feed into isolator.Start to look into the potential phase within the 5th day after inoculation, collect the progress dissections of the 24h after the minimum potential phase and collect caecum, carry out tissue rubbing, four groups respectively with saturated saline floatation, sucrose solution floating method, saturation sulfuric acid solution floating method, solution floating method of the present invention, contrasted.Calculate the rate of recovery.
Table saturated saline floatation and solution of the present invention collect excrement egg capsule contrast table
Conclusion：Same group of tissue saturated saline floatation, sucrose solution floating method, the egg capsule rate of recovery of saturation Adlerika floating method are not less than 70%；And the rate of recovery of solution floating method egg capsule of the present invention has exceeded 90%, illustrate the method that present invention group can substitute the purifying of other egg capsules completely.
Claims (3)
1. a kind of improve solution of the coccidian oocyst rate of recovery and preparation method thereof in chicken tissues, its
It is characterised by：The component and its ratio of weight and number of the solution be：
2. the coccidian oocyst rate of recovery in a kind of raising chicken tissues according to claim 1
Solution and preparation method thereof, it is characterised in that：The solution component and its optimal parts by weight score
It is not：
3. the coccidian oocyst rate of recovery in a kind of a kind of raising chicken tissues as claimed in claim 1
Solution and preparation method thereof, it is characterised in that：The step of preparation method is：
(1) disodium ethylene diamine tetraacetate, aluminium polychloride, melon are weighed successively according to weight proportion
That glue hydroxypropyl-trimethyl ammonium chloride, sodium hypochlorite are put into Agitation Tank, then add aqua sterilisa
To do not had it is above-mentioned feed intake, at room temperature soak 1 hour after, be further continued for adding remaining aqua sterilisa,
Stir, be completely dissolved to feeding intake, then solution A is obtained with 0.45um polyether sulfone filter element filterings,
Storage is into Agitation Tank；
(2) Agitation Tank heating is advisable with steam pressure 0.05Mpa, evaporator is discharged for 15 minutes
Condensed water once, every two hours discharges the condensation vapour of heater once.Indoor Natural is cooled down, standby
With obtaining solution B standby；
(3) with resulting solution B in 0.22um polyether sulfone filter element filtering steps (2), and adjust molten
Liquid pH value is stirred 15 minutes to 6.0-7.0, again with 0.22um polyether sulfone filter element filterings,
Solution C is obtained, it is directly entered bottle placer by pipeline；
(4) resulting solution C in step (3) is filled in the medical vials after drying, i.e.,
It can obtain a kind of solution for improving the coccidian oocyst rate of recovery in chicken tissues.
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| CN201610065737.0A CN107022491B (en) | 2016-01-30 | 2016-01-30 | Solution for improving recovery rate of coccidian oocysts in chicken tissues and preparation method thereof |
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| CN201610065737.0A CN107022491B (en) | 2016-01-30 | 2016-01-30 | Solution for improving recovery rate of coccidian oocysts in chicken tissues and preparation method thereof |
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| CN107022491B CN107022491B (en) | 2020-11-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733079A (en) * | 2020-07-08 | 2020-10-02 | 佛山市正典生物技术有限公司 | Separation composition, preparation method thereof and chicken coccidian oocyst separation method using same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046533A1 (en) * | 1997-04-14 | 1998-10-22 | Zenon Environmental, Inc. | Process for purifying water |
| CN1309179A (en) * | 2000-02-18 | 2001-08-22 | 广东省农业科学院兽医研究所 | In-vitro cell culture model of chicken coccidian and its application |
| CN103881913A (en) * | 2014-03-07 | 2014-06-25 | 广东省农业科学院动物卫生研究所 | Industria production method for separating and purifying chicken eimeria coccidium oocyst |
-
2016
- 2016-01-30 CN CN201610065737.0A patent/CN107022491B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046533A1 (en) * | 1997-04-14 | 1998-10-22 | Zenon Environmental, Inc. | Process for purifying water |
| CN1309179A (en) * | 2000-02-18 | 2001-08-22 | 广东省农业科学院兽医研究所 | In-vitro cell culture model of chicken coccidian and its application |
| CN103881913A (en) * | 2014-03-07 | 2014-06-25 | 广东省农业科学院动物卫生研究所 | Industria production method for separating and purifying chicken eimeria coccidium oocyst |
Non-Patent Citations (1)
| Title |
|---|
| 朱冠等: "用改良韦氏法纯化鸡球虫卵囊", 《动物学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733079A (en) * | 2020-07-08 | 2020-10-02 | 佛山市正典生物技术有限公司 | Separation composition, preparation method thereof and chicken coccidian oocyst separation method using same |
| CN111733079B (en) * | 2020-07-08 | 2021-09-07 | 佛山市正典生物技术有限公司 | Separation composition, preparation method thereof and chicken coccidian oocyst separation method using same |
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