CN108948115A - A kind of method of high efficiency extraction Cordyceps militaris adenosine - Google Patents
A kind of method of high efficiency extraction Cordyceps militaris adenosine Download PDFInfo
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- CN108948115A CN108948115A CN201811199930.9A CN201811199930A CN108948115A CN 108948115 A CN108948115 A CN 108948115A CN 201811199930 A CN201811199930 A CN 201811199930A CN 108948115 A CN108948115 A CN 108948115A
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- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/167—Purine radicals with ribosyl as the saccharide radical
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
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Abstract
The invention belongs to Cordyceps militaris effective component extractive technique fields, and in particular to the extracting method of adenosine in a kind of high efficiency extraction Cordyceps militaris.The method of the present invention for extracting Cordyceps militaris adenosine, on the basis of in the prior art using water as Extraction solvent, further adding a selected amount of includes one of laccase or zytase, and the compound collecting enzyme of one of cellulase or pectase carries out enzymolysis and extraction, effectively increases the recovery rate of adenosine in Cordyceps militaris;The method of the present invention for extracting adenosine in Cordyceps militaris effectively increases the dissolution and extraction of cordyceps sinensis adenosine by way of polyethylene glycol is added in pulping stage;More preferably, in the method for the invention, before extracting, the fruiting bodies of cordyceps militaris is dried in such a way that gradient is dry, utilize the temperature difference in each stage, the shell-broken effect of the fruiting bodies of cordyceps militaris is further improved, the dissolution of adenosine has been effectively facilitated, it helps improves the recovery rate of adenosine.
Description
Technical field
The invention belongs to Cordyceps militaris effective component extractive technique fields, and in particular to adenosine in a kind of high efficiency extraction Cordyceps militaris
Extracting method.
Background technique
Cordyceps militaris (Cordyceps militaris (L.) Link) also known as northern Chinese caterpillar Fungus, are under the jurisdiction of Eumycota
(Eumycota), Ascomycota (Ascomycota), Ascomycotina (Ascomycotinia), excrement shell Gammaproteobacteria
(Sordariomycetes), meat seat bacterium subclass (Hypocreomycetidae), Hypocreales (Hypocreales), cordyceps sinensis section
(Cordycipitaceae), the type sepecies of Cordyceps (Cordyceps).Cordyceps militaris usually has similar life with cordyceps sinensis
Object active constituent mainly includes cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, ergosterol, amino acid, nucleosides material etc., Cordyceps militaris
The effect of it is also similar to cordyceps sinensis, be the good substitute of cordyceps sinensis.Cordyceps militaris is a kind of natural resources, shows the world
Property distribution, but quantity is seldom, and majority is cultivated by artificial plantation.In recent years, chemical component and pharmacology of the mankind to artificial aweto
Activity conducts extensive research, the results showed that, the chemical component of artificial aweto, pharmacological effect and natural cs are essentially identical,
Wherein Cordyceps sinensis polysaccharide, cordycepin content be also more than natural cs.Largely modern pharmacological studies have shown that Cordyceps militaris not only has
There is special nutritive value, and has apparent medical value.
Adenosine is a kind of endogenous purine nucleoside acid, belongs to nucleosides material, is one of effective component of cordyceps sinensis, is
The direct premise of cordycepin itself also has with extensive physiological activity, in Cordyceps militaris the content of adenosine mostly 0.01% with
On, it is similar to cordyceps sinensis, it is even higher.So far, separation identification has 11 ucleosides from cordyceps sinensis and fermented hypha
Compound, including adenine, adenosine, uracil, uridine, guanine, guanosine, hypoxanthine, inosine, thymus gland are phonetic
Pyridine, thymidine and uracil deoxyribonucleoside etc..Cordyceps sinensis adenosine can improve cardiovascular and cerebrovascular blood circulation, prevent the heart
Restrain it is not normal, reduce blood pressure;The release of norepinephrine can be reduced, cordyceps sinensis adenosine weakens catecholamines in boys to heart
Excitation stimulates, thus cardioprotection;Important protection is more played in myocardial ischemic preconditioning, ischemical reperfusion injury
Effect;Cordyceps sinensis adenosine also has very strong antiplatelet aggregative activity, is a kind of very strong vaso-active substance, can make body circulation
Blood vessel dilatation causes blood pressure stabilization in normal low-level.As it can be seen that cordyceps sinensis adenosine is in antiviral, antibacterial, prophylactic treatment brain blood
Bolt, cerebral hemorrhage inhibit Platelet congregation, and prevent thrombosis, have preferable effect.But either natural cs
Or artificial aweto, the content of cordyceps sinensis adenosine is very low, causes certain difficulty for the extraction of cordyceps sinensis adenosine.Existing skill
Enzyme process is mostly used to extract cordyceps sinensis adenosine in art, but the problem lower in the prevalence of extraction efficiency.
Summary of the invention
For this purpose, technical problem to be solved by the present invention lies in a kind of method of adenosine in high efficiency extraction Cordyceps militaris is provided,
To solve the problems, such as that cordyceps sinensis adenosine recovery rate is lower in the prior art.
In order to solve the above technical problems, in a kind of high efficiency extraction Cordyceps militaris of the present invention adenosine method, including such as
Lower step:
(1) it takes fruiting bodies of cordyceps militaris dry, and adds water slurrying, Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme is added into gained Cordyceps militaris slurries to extract, obtains the extract of adenosine containing cordyceps sinensis.
In the step (2), the compound collecting enzyme include one of laccase or zytase and cellulase or
One of pectase.
Preferably, the compound collecting enzyme includes:
Mass ratio is the laccase and cellulase of 1-2:1-3;Alternatively,
Mass ratio is the laccase and pectase of 1-2:2-4;Alternatively,
Mass ratio is the zytase and cellulase of 2-3:1-2;Alternatively,
Mass ratio is the zytase and pectase of 2-3:3-4.
Preferably, the additional amount of the compound collecting enzyme accounts for the 3-8wt% of the fruiting bodies of cordyceps militaris quality.
Preferably, in the pulping stage, the solid-liquid ratio of the fruiting bodies of cordyceps militaris and water is 1:20-30g/mL.
More preferably, in the pulping stage, further include the steps that polyethylene glycol is added in water carries out slurrying.
More preferably, the polyethylene glycol includes polyethylene glycol 400.
More preferably, in the pulping stage, the mass concentration for controlling the Aqueous Solutions of Polyethylene Glycol is 10-20wt%.
It more preferably, further include that the fruiting bodies of cordyceps militaris is subjected to gradient drying in 40-80 DEG C in the step (1)
Step.
Specifically, the gradient drying steps specifically include: the initial drying temperature for controlling the fruiting bodies of cordyceps militaris is
40-50 DEG C, and heating drying is carried out to the fruiting bodies of cordyceps militaris constant weight with the program of 5 DEG C/10min, control the dry step
Rapid temperature is no more than 80 DEG C.
It is of the present invention extract Cordyceps militaris adenosine method, in the prior art using water as Extraction solvent on the basis of,
Further adding a selected amount of includes answering for one of one of laccase or zytase and cellulase or pectase
It closes and extracts enzyme progress enzymolysis and extraction, effectively increase the recovery rate of adenosine in Cordyceps militaris;Gland in extraction Cordyceps militaris of the present invention
The method of glycosides effectively increases the dissolution and extraction of cordyceps sinensis adenosine by way of polyethylene glycol is added in pulping stage;More
Excellent, in the method for the invention, before extracting, the fruiting bodies of cordyceps militaris is carried out in such a way that gradient is dry
It is dry, using the temperature difference in each stage, the shell-broken effect of the fruiting bodies of cordyceps militaris is further improved, adenosine has been effectively facilitated
Dissolution, it helps improve the recovery rate of adenosine.
Specific embodiment
Embodiment 1
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 20L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 30g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the laccase and cellulase for 1:3;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 2
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 30L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 80g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the laccase and cellulase for 2:1;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 3
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 25L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 50g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the laccase and cellulase for 1.5:2;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 4
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 20L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 30g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the laccase and pectase for 1:4;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 5
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 30L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 80g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the laccase and pectase for 2:2;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 6
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 25L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 50g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the laccase and pectase for 1.5:3;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 7
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 20L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 30g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the zytase and cellulase for 2:2;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 8
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 30L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 80g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the zytase and cellulase for 3:1;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 9
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 25L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 50g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the zytase and cellulase for 2.5:1.5;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 10
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 20L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 30g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the zytase and pectase for 2:4;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 11
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 30L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 80g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the zytase and pectase for 3:3;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 12
The method of adenosine, includes the following steps: in high efficiency extraction Cordyceps militaris of the present invention
(1) fruiting bodies of cordyceps militaris is taken to be dried in 50 DEG C to constant temperature, the fruiting bodies of cordyceps militaris 1kg after taking above-mentioned drying,
And water 25L is added to carry out conventional slurrying, and Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme 50g is added into gained Cordyceps militaris slurries and extracts 2h, the compound collecting enzyme includes matter
Amount is than the zytase and pectase for 2.5:3.5;Obtain the extract of adenosine containing cordyceps sinensis.
Embodiment 13
The method of adenosine is only that with embodiment 3, difference in high efficiency extraction Cordyceps militaris of the present invention, the slurrying
Step is to carry out slurrying using the polyethylene glycol 400 aqueous solution of mass concentration 10wt%.
Embodiment 14
The method of adenosine is only that with embodiment 6, difference in high efficiency extraction Cordyceps militaris of the present invention, the slurrying
Step is to carry out slurrying using the polyethylene glycol 400 aqueous solution of mass concentration 20wt%.
Embodiment 15
The method of adenosine is only that with embodiment 9, difference in high efficiency extraction Cordyceps militaris of the present invention, the slurrying
Step is to carry out slurrying using the polyethylene glycol 400 aqueous solution of mass concentration 15wt%.
Embodiment 16
The method of adenosine is only that with embodiment 12, difference in high efficiency extraction Cordyceps militaris of the present invention, the system
Starching step is to carry out slurrying using the polyethylene glycol 400 aqueous solution of mass concentration 15wt%.
Embodiment 17
The method of adenosine is only that with embodiment 13, difference in high efficiency extraction Cordyceps militaris of the present invention, the pupa
The dry step of cordyceps militaris sporocarp is the dry process of gradient, specifically includes: controlling the initial drying of the fruiting bodies of cordyceps militaris
Temperature is 40 DEG C, and carries out heating drying to the fruiting bodies of cordyceps militaris constant weight with the program of 5 DEG C/10min, controls the drying
The temperature of step is no more than 80 DEG C.
Embodiment 18
The method of adenosine is only that with embodiment 14, difference in high efficiency extraction Cordyceps militaris of the present invention, the pupa
The dry step of cordyceps militaris sporocarp is the dry process of gradient, specifically includes: controlling the initial drying of the fruiting bodies of cordyceps militaris
Temperature is 50 DEG C, and carries out heating drying to the fruiting bodies of cordyceps militaris constant weight with the program of 5 DEG C/10min, controls the drying
The temperature of step is no more than 80 DEG C.
Embodiment 19
The method of adenosine is only that with embodiment 15, difference in high efficiency extraction Cordyceps militaris of the present invention, the pupa
The dry step of cordyceps militaris sporocarp is the dry process of gradient, specifically includes: controlling the initial drying of the fruiting bodies of cordyceps militaris
Temperature is 45 DEG C, and carries out heating drying to the fruiting bodies of cordyceps militaris constant weight with the program of 5 DEG C/10min, controls the drying
The temperature of step is no more than 80 DEG C.
Embodiment 20
The method of adenosine is only that with embodiment 16, difference in high efficiency extraction Cordyceps militaris of the present invention, the pupa
The dry step of cordyceps militaris sporocarp is the dry process of gradient, specifically includes: controlling the initial drying of the fruiting bodies of cordyceps militaris
Temperature is 45 DEG C, and carries out heating drying to the fruiting bodies of cordyceps militaris constant weight with the program of 5 DEG C/10min, controls the drying
The temperature of step is no more than 80 DEG C.
Comparative example 1
The method of adenosine is to be extracted using method in the prior art in extraction Cordyceps militaris described in this comparative example, i.e., directly
The water that equivalent is added carries out slurrying extraction.
Comparative example 2
The method of adenosine is to be extracted using method in the prior art in extraction Cordyceps militaris described in this comparative example, i.e., directly
The 60wt% ethanol solution that equivalent is added carries out slurrying extraction.
Comparative example 3
The method of adenosine is to be extracted using method in the prior art in extraction Cordyceps militaris described in this comparative example, that is, is being added
After water pulping stage, it is added and carries out enzymolysis and extraction with the cellulase of the compound collecting enzyme equivalent.
Comparative example 4
The method of adenosine is to be extracted using method in the prior art in extraction Cordyceps militaris described in this comparative example, that is, is being added
After water pulping stage, it is added and carries out enzymolysis and extraction with the pectase of the compound collecting enzyme equivalent.
Comparative example 5
The method of adenosine is to be extracted using method in the prior art in extraction Cordyceps militaris described in this comparative example, that is, is being added
After water pulping stage, it is added and carries out enzymolysis and extraction with the albumen of the compound collecting enzyme equivalent.
Experimental example
The extracting solution collected in above-described embodiment 1-20 and comparative example 1-5 is taken to carry out the detection of cordyceps sinensis adenosine content respectively,
It is specific to be measured using HPLC method conventional in the prior art, it can be according to the inspection recorded in Chinese patent CN102558265A
Survey method extracts the measurement of cordyceps sinensis adenosine content in liquid, and opposite with the extracted amount that this calculates cordyceps sinensis adenosine in the extracting solution
In the extraction efficiency of fruiting bodies of cordyceps militaris, it is recorded in the following table 1.
1 Cordyceps militaris adenosine recovery rate result of table
It can be seen that the method for the present invention for extracting Cordyceps militaris adenosine from upper table data, made in the prior art with water
On the basis of Extraction solvent, further adding a selected amount of includes one of laccase or zytase and cellulase
Or the compound collecting enzyme of one of pectase carries out enzymolysis and extraction, effectively increases the recovery rate of adenosine in Cordyceps militaris;This hair
The bright method for extracting adenosine in Cordyceps militaris effectively increases worm by way of polyethylene glycol is added in pulping stage
The dissolution and extraction of careless adenosine;More preferably, in the method for the invention, before extracting, in such a way that gradient is dry
The fruiting bodies of cordyceps militaris is dried, using the temperature difference in each stage, further improves the broken of the fruiting bodies of cordyceps militaris
Wall effect has effectively facilitated the dissolution of adenosine, it helps improves the recovery rate of adenosine.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. a kind of method of adenosine in high efficiency extraction Cordyceps militaris, which comprises the steps of:
(1) it takes fruiting bodies of cordyceps militaris dry, and adds water slurrying, Cordyceps militaris slurries are made, it is spare;
(2) compound collecting enzyme is added into gained Cordyceps militaris slurries to extract, obtains the extract of adenosine containing cordyceps sinensis.
2. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 1, which is characterized in that in the step (2),
The compound collecting enzyme includes one of laccase or zytase and one of cellulase or pectase.
3. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 2, which is characterized in that the compound collecting enzyme
Include:
Mass ratio is the laccase and cellulase of 1-2:1-3;Alternatively,
Mass ratio is the laccase and pectase of 1-2:2-4;Alternatively,
Mass ratio is the zytase and cellulase of 2-3:1-2;Alternatively,
Mass ratio is the zytase and pectase of 2-3:3-4.
4. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 1-3, which is characterized in that described multiple
The additional amount for closing extraction enzyme accounts for the 3-8wt% of the fruiting bodies of cordyceps militaris quality.
5. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 1-4, which is characterized in that the system
It starches in step, the solid-liquid ratio of the fruiting bodies of cordyceps militaris and water is 1:20-30g/mL.
6. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 1-5, which is characterized in that the system
It starches in step, further includes the steps that polyethylene glycol is added in water carries out slurrying.
7. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 6, which is characterized in that the polyethylene glycol packet
Include polyethylene glycol 400.
8. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 6 or 7, which is characterized in that the slurrying step
In rapid, the mass concentration for controlling the Aqueous Solutions of Polyethylene Glycol is 10-20wt%.
9. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 1-8, which is characterized in that the step
Suddenly in (1), include the steps that the fruiting bodies of cordyceps militaris is dry in 40-80 DEG C of progress gradient.
10. the method for adenosine in high efficiency extraction Cordyceps militaris according to claim 9, which is characterized in that the gradient is dry
Step specifically includes: the initial drying temperature for controlling the fruiting bodies of cordyceps militaris is 40-50 DEG C, and with the program of 5 DEG C/10min
It is dry to the fruiting bodies of cordyceps militaris constant weight to carry out heating, the temperature for controlling the drying steps is no more than 80 DEG C.
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Cited By (3)
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CN109645080A (en) * | 2019-01-24 | 2019-04-19 | 安徽华米生物科技有限公司 | A kind of cordyceps sinensis biscuit and preparation method thereof |
CN113480587A (en) * | 2021-05-20 | 2021-10-08 | 徐州工程学院 | Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp |
CN114163485A (en) * | 2021-11-11 | 2022-03-11 | 浏阳朗林生物科技有限公司 | Preparation method and application of cordyceps militaris extract |
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CN109645080A (en) * | 2019-01-24 | 2019-04-19 | 安徽华米生物科技有限公司 | A kind of cordyceps sinensis biscuit and preparation method thereof |
CN113480587A (en) * | 2021-05-20 | 2021-10-08 | 徐州工程学院 | Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp |
CN113480587B (en) * | 2021-05-20 | 2024-05-03 | 湖南合健生物科技有限公司 | Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies |
CN114163485A (en) * | 2021-11-11 | 2022-03-11 | 浏阳朗林生物科技有限公司 | Preparation method and application of cordyceps militaris extract |
CN114163485B (en) * | 2021-11-11 | 2023-10-13 | 湖南朗林生物资源股份有限公司 | Preparation method and application of Cordyceps militaris extract |
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