CN107017144B - A kind of microscope thermal station - Google Patents
A kind of microscope thermal station Download PDFInfo
- Publication number
- CN107017144B CN107017144B CN201710323744.0A CN201710323744A CN107017144B CN 107017144 B CN107017144 B CN 107017144B CN 201710323744 A CN201710323744 A CN 201710323744A CN 107017144 B CN107017144 B CN 107017144B
- Authority
- CN
- China
- Prior art keywords
- temperature control
- heating
- control case
- bottom plate
- thermal station
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000010438 heat treatment Methods 0.000 claims abstract description 63
- 239000011521 glass Substances 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims description 6
- 229910000838 Al alloy Inorganic materials 0.000 claims description 4
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims description 3
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 4
- 210000000130 stem cell Anatomy 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 210000000287 oocyte Anatomy 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/02—Details
- H01J37/20—Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support
-
- H—ELECTRICITY
- H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
- H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
- H05B3/00—Ohmic-resistance heating
- H05B3/20—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater
- H05B3/22—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible
- H05B3/28—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible heating conductor embedded in insulating material
- H05B3/30—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible heating conductor embedded in insulating material on or between metallic plates
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of microscope thermal station, including temperature control case and warm table, AC/DC power supply and temperature controller are equipped in the temperature control case, the rear end of the temperature control case is equipped with power outlet, front end is equipped with heating socket and switch, the front end of the temperature controller are the temperature control panel for protruding from temperature control case front end face；The warm table includes successively folding the bottom plate set, soft adiabator layer, heating film and heating platform from the bottom up, the lower section of the bottom plate is equipped with support leg, the heating film is connected by heating cable with the heating socket of temperature control case, the warm table is equipped with sight vane, is embedded with combining glass in the sight vane.The present invention is able to satisfy embryo or egg mother cell steady temperature operation needs, and can have enough operating platforms, facilitates operation, durable, easy to carry.
Description
Technical field
The invention belongs to electron microscope accessory technical fields, and in particular to a kind of microscope thermal station.
Background technique
The in vitro operation of external egg mother cell, embryo and some special cells requires to operate at a suitable temperature,
Especially oocyte maturation early period picks up ovum process, the stoning of body-cell neucleus transplanting, with during Somatic Fusion etc., all needs
It will (37-38.5 DEG C) operation at a suitable temperature.It is suitable in order to obtain due to the typically no included thermal station of Stereo microscope
Operation temperature then needs to make suitable thermal station to measure for Stereo microscope.This thermal station should can guarantee stationary temperature, again
Guarantee not influence observing effect under Stereo microscope, not only to meet operating table surface width when use, while can be suitble to again
Microscopical pedestal.Although the thermal station now with some imports is able to satisfy the above demand, the specification of these thermal station is all special
It is fixed, it can be placed in just under the microscope of certain model, their whole operation table top is narrower, is not able to satisfy somatic cell nuclear shifting
Operational requirements when plant, particularly when handmade cloning.In addition, some microscope hot stages are then glass materials, although to microscope
Lower observation is more convenient, but its material is frangible, it is easy to be destroyed by " experiment new hand ".Therefore there is an urgent need to produce a kind of microscope
Thermal station can meet broader operating table surface and securely solid simultaneously, and be able to maintain accurate constant temperature.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of microscope thermal station, have the operation for keeping accurate and constant
Temperature, broad comfortable operating table surface is durable, easy to carry.
In order to solve the above technical problems, the embodiment of the present invention provides a kind of microscope thermal station, including temperature control case and
Warm table, the temperature control case is interior to be equipped with AC/DC power supply and temperature controller, and the rear end of the temperature control case is equipped with power outlet, preceding
End is equipped with heating socket and switch, the front end of the temperature controller are the temperature control panel for protruding from temperature control case front end face；
The warm table includes successively folding the bottom plate set, soft adiabator layer, heating film and heating platform from the bottom up,
The lower section of the bottom plate is equipped with support leg, and the heating film is connected by heating cable with the heating socket of temperature control case, described
Warm table is equipped with sight vane, is embedded with combining glass in the sight vane.
Wherein, the front end of the temperature control case is additionally provided with temperature sensor socket, and the lower end of the heating platform is equipped with recessed
Slot, temperature sensor is equipped in the groove, and the temperature sensor passes through the temperature sensing of sensor wire and temperature control case
Device socket is connected.
Further, cabling channel is offered on the lower end surface of the heating platform, the sensor wire is arranged in cabling channel
It is interior.
Above-mentioned microscope thermal station further includes fixed frame, the upper end of the fixed frame and the fixed company of heating platform
It connects, lower end is fixedly connected with bottom plate.
Preferably, the fixed frame is formed by four structure side slats are end to end, the upper end of each structure side slat
Face is fixedly connected with the lower surface of heating platform by screw, and the lower end surface of each structure side slat and the upper surface of bottom plate are logical
It crosses screw to be fixedly connected, the heating film and soft adiabator layer between the heating platform and bottom plate are enclosed set on fixed frame
In frame.
Wherein, it is equipped with the glass seal being fixedly connected with lower surface of base plate below the sight vane, the glass seal
Upper end is inserted into sight vane, and the combining glass is set to the inside of the top of glass seal, sight vane.
Preferably, the support leg is telescopic support foot, can be according to Microscopii height or experiment demand tune
Section height.
Preferably, for aluminium alloy 6063, the soft adiabator layer is the material that the heating platform and bottom plate use
Alumina silicate fibre layer.The combining glass selects high light transmission heat-resistant, high strength degree glass.
The advantageous effects of the above technical solutions of the present invention are as follows: the present invention is able to satisfy embryo or egg mother cell constant temperature
Degree operation needs, and can have enough operating platforms, facilitates operation, durable, easy to carry.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the embodiment of the present invention one；
Fig. 2 is the structural exploded view of temperature control case in embodiment one；
Fig. 3 is the structural exploded view of embodiment one；
Fig. 4 is the top view of warm table in embodiment one；
Fig. 5 be Fig. 4 in along D-D cross-sectional view；
Fig. 6 is the partial enlarged view in Fig. 5 at A；
Fig. 7 is the control flow block diagram of the embodiment of the present invention one.
Description of symbols:
1, temperature control case；100, AC/DC power supply；101, temperature controller；102, power outlet；103, socket is heated；104, warm
Spend sensor socket；105, it switchs；106, temperature control panel；2, warm table；200, bottom plate；201, soft adiabator layer；202,
Heating film；203, heating platform；204, support leg；205, sight vane；206, combining glass；207, glass seal；3, heater wire
Cable；4, temperature sensor；5, sensor wire；6, structure side slat.
Specific embodiment
To keep the technical problem to be solved in the present invention, technical solution and advantage clearer, below in conjunction with attached drawing and tool
Body embodiment is described in detail.
A kind of microscope thermal station, including temperature control case 1, warm table 2 and connecting line, control flow block diagram such as Fig. 7 institute
Show.
As shown in Figure 1 and Figure 2, AC/DC power supply 100 and temperature controller 101, the temperature control case are equipped in the temperature control case 1
1 rear end is equipped with power outlet 102, and front end is equipped with heating socket 103, temperature sensor socket 104 and switch 105, the temperature
The front end for controlling device 101 is the temperature control panel 106 for protruding from 1 front end face of temperature control case.It is therein to have AC/DC power supply 100 be in electricity
It keeps persistently inputting the 220V use for laboratory power supply of AC/DC power supply 100 under the connection of supply socket 102, and passes through AC/DC power supply
The 24V DC power supply that 100 conversion needs the output of heating film 202.Temperature controller 101 is commercially available commercially available temperature control instrument, including
Temperature measuring device, humidostat, workable air temperature system etc..
As shown in figs. 3 and 5, the warm table 2 includes successively folding bottom plate 200, the soft adiabator layer set from the bottom up
201, heating film 202 and heating platform 203, the lower section of the bottom plate 200 are equipped with support leg 204, and the heating film 202 is by adding
Hot line cable 3 is connected with the heating socket 103 of temperature control case 1, and the warm table 2 is equipped with circular sight vane 205(and sees Fig. 4),
Position of the sight vane 205 on warm table is set according to be applicable in microscope, embedded with perspective glass in the sight vane 205
Glass 206.
The material that above-mentioned heating platform and bottom plate uses is aluminium alloy 6063, light easy processing, and heating conduction is excellent.It is soft
Property adiabator layer be alumina silicate fibre layer, good heat insulating is soft, compressible, and heating film is caused to be bonded with heating platform tightly
It is close.
In the present embodiment, the glass seal 207 being fixedly connected with 200 lower surface of bottom plate is equipped with below the sight vane 205,
The glass seal 207 upper end insertion sight vane 205 in, the combining glass 206 be set to glass seal 207 top, thoroughly
The inside of window 205.The combining glass 206 selects high light transmission heat-resistant, high strength degree glass.
As shown in fig. 6, the position of the lower end of the heating platform 203, neighbouring sight vane 205 is equipped with groove, in the groove
It is equipped with temperature sensor 4, which is provided with temp probe, the temperature sensor 4 passes through sensor wire 5 and temperature control case 1
Temperature sensor socket 104 be connected, can accurate real-time temperature control, error is within the scope of ± 0.5 oC.
As shown in figure 5, offering cabling channel on the lower end surface of the heating platform 203, the sensor wire 5 is arranged in
In cabling channel.
Above-mentioned microscope thermal station further includes fixed frame, and the upper end of the fixed frame and heating platform 203 are fixed
Connection, lower end is fixedly connected with bottom plate 200, and there is position corresponding with cabling channel on fixed frame passes through for sensor wire 5
Through-hole.
As shown in figure 3, the fixed frame is formed by four structure side slats 6 are end to end, on each structure side slat 6 every
5cm screws hole, and the upper surface of each structure side slat 6 is fixedly connected with the lower surface of heating platform 203 by screw, often
The lower end surface of the one structure side slat 6 is fixedly connected with the upper surface of bottom plate 200 by screw, and the heating platform 203 is located at
Heating film 202 and soft adiabator layer 201 between bottom plate 200 are enclosed in fixed frame, and structure side slat and heating
Soft adiabator layer and heating film are fitted tightly on the bottom surface of heating platform by the connection between platform and bottom plate, thus
Obtain good heating properties.
The fixed frame can also use following structures: be formed by four structure side slats are end to end, the structure side
The inside of item is equipped with card slot.It screws hole, the upper surface of each structure side slat and heating on each structure side slat every 5cm
The lower surface of platform is fixedly connected by screw, and the lower end surface of each structure side slat and the upper surface of bottom plate are solid by screw
Fixed connection, the side of heating film and soft adiabator layer between the heating platform and bottom plate is arranged in card slot.
The support leg 204 is telescopic support foot, can be adjusted according to Microscopii height or experiment demand high
Degree.
It is adjustable that the present embodiment may be designed to link position of the support leg in lower surface of base plate, such as in the lower surface of bottom plate
Upper design multiple groups threaded hole group, support leg realize that position is adjustable by being connected from different threaded hole groups；It can also be under bottom plate
Transverse direction and/or the vertical chute are offered on surface, support leg is fixed after sliding to suitable position in sliding slot.
The embodiment of the present invention also provides a kind of electron microscope using above-mentioned microscope thermal station, including but not limited to
Stereo microscope, the Stereo microscope are equipped with objective table, object lens and light source, and the microscope hot stage is set on objective table
Side, between object lens and light source, observing object is set between sight vane and object lens the sight vane.
The present invention is to provide a kind of portable microscope thermal station, including warm table, control cabinet and control cabinet with
Connection cables between warm table, warm table among the panel of 6063 material of two panels aluminium alloy (heating platform and bottom plate) mainly by pressing from both sides
Heating film and soft thermal insulation material constitute, and temperature sensor spy is set in the middle position that heating platform be bonded with heating film
Head and sensor wire, so as to more accurately obtain the temperature of heating platform and pass to control cabinet temperature control panel, into one
Step is constant by the temperature of AC/DC power supply output electric energy real-time control heating platform.And AC/DC power supply can be in the input laboratory 220V
With 24V DC power supply is exported in the case where power supply, thus safer.
Warm table size of the invention not only can need design size according to the size and actual use of Microscope base,
And perspective hole site can also change according to the needs of microscope light source position.Therefore, the microscope is with thermal station
Embryo or egg mother cell steady temperature operation needs are able to satisfy, for example pick up ovum process, body cell in oocyte maturation early period
It can provide 38.5o constant temperature in the operation such as stoning of nuclear transfer, and can have enough operating platforms, facilitate operation.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, without departing from the principles of the present invention, it can also make several improvements and retouch, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (7)
1. a kind of microscope thermal station, which is characterized in that including temperature control case and warm table, AC/ is equipped in the temperature control case
DC power supply and temperature controller, the rear end of the temperature control case are equipped with power outlet, and front end is equipped with heating socket and switch, the temperature control
The front end of device is the temperature control panel for protruding from temperature control case front end face；
The warm table includes successively folding the bottom plate set, adiabator layer, heating film and heating platform from the bottom up, the bottom plate
Lower section be equipped with support leg, the heating film is connected by heating cable with the heating socket of temperature control case, on the warm table
Equipped with sight vane, combining glass is embedded in the sight vane；
Link position of the support leg in lower surface of base plate is adjustable；
The microscope thermal station further includes fixed frame, and the upper end of the fixed frame is fixedly connected with heating platform, lower end
It is fixedly connected with bottom plate；The fixed frame is formed by four structure side slats are end to end, the upper end of each structure side slat
Face is fixedly connected with the lower surface of heating platform by screw, and the lower end surface of each structure side slat and the upper surface of bottom plate are logical
It crosses screw to be fixedly connected, the heating film and soft adiabator layer between the heating platform and bottom plate are enclosed set on fixed frame
In frame.
2. microscope thermal station according to claim 1, which is characterized in that the front end of the temperature control case is additionally provided with temperature
Sensor socket, the lower end of the heating platform are equipped with groove, are equipped with temperature sensor, the temperature sensor in the groove
It is connected by sensor wire with the temperature sensor socket of temperature control case.
3. microscope thermal station according to claim 2, which is characterized in that offered on the lower end surface of the heating platform
Cabling channel, the sensor wire are arranged in cabling channel.
4. microscope thermal station according to claim 1, which is characterized in that be equipped with and bottom plate following table below the sight vane
The upper end of the glass seal that face is fixedly connected, the glass seal is inserted into sight vane, and the combining glass is set to glass seal
Top, sight vane inside.
5. microscope thermal station according to claim 1, which is characterized in that the support leg is telescopic support foot.
6. microscope thermal station according to claim 1, which is characterized in that the material that the heating platform and bottom plate use
For aluminium alloy 6063, the soft adiabator layer is alumina silicate fibre layer.
7. microscope thermal station according to claim 1 or 4, which is characterized in that the combining glass selects high light transmission resistance to
Warm high strength glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323744.0A CN107017144B (en) | 2017-05-10 | 2017-05-10 | A kind of microscope thermal station |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323744.0A CN107017144B (en) | 2017-05-10 | 2017-05-10 | A kind of microscope thermal station |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107017144A CN107017144A (en) | 2017-08-04 |
| CN107017144B true CN107017144B (en) | 2019-07-09 |
Family
ID=59449780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710323744.0A Expired - Fee Related CN107017144B (en) | 2017-05-10 | 2017-05-10 | A kind of microscope thermal station |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107017144B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2051746U (en) * | 1989-04-12 | 1990-01-24 | 山东省济南卫生学校 | Warm keeping apparatus for microscopic observation of biological alive specimen |
| JPH0921960A (en) * | 1995-07-05 | 1997-01-21 | Olympus Optical Co Ltd | Heat insulating device for microscope |
| JPH1138329A (en) * | 1997-07-22 | 1999-02-12 | Tokai Hit:Kk | Specimen temperature controller for microscope |
| JP2004177846A (en) * | 2002-11-29 | 2004-06-24 | Tokai Hit:Kk | Transparent heating device for microscopic observation |
| JP2006025612A (en) * | 2004-07-12 | 2006-02-02 | Nikon Corp | Pyrostat |
| WO2009041200A1 (en) * | 2007-09-27 | 2009-04-02 | Tokai Hit Co., Ltd. | Sample stage device having temperature gradient |
| JP4262390B2 (en) * | 2000-06-06 | 2009-05-13 | 株式会社東海ヒット | Specimen temperature controller for microscope |
| CN201454570U (en) * | 2009-09-10 | 2010-05-12 | 王敏康 | Large-plane temperature control heating table |
| CN103861676A (en) * | 2014-03-18 | 2014-06-18 | 青岛汉源微电子有限公司 | Ultra-thin precision temperature control heating platform |
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| CN206834150U (en) * | 2017-05-10 | 2018-01-02 | 南京农业大学 | A kind of microscope thermal station |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW581119U (en) * | 2001-12-19 | 2004-03-21 | Univ Nat Cheng Kung | Petri dish for microscope |
-
2017
- 2017-05-10 CN CN201710323744.0A patent/CN107017144B/en not_active Expired - Fee Related
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2051746U (en) * | 1989-04-12 | 1990-01-24 | 山东省济南卫生学校 | Warm keeping apparatus for microscopic observation of biological alive specimen |
| JPH0921960A (en) * | 1995-07-05 | 1997-01-21 | Olympus Optical Co Ltd | Heat insulating device for microscope |
| JPH1138329A (en) * | 1997-07-22 | 1999-02-12 | Tokai Hit:Kk | Specimen temperature controller for microscope |
| JP4262390B2 (en) * | 2000-06-06 | 2009-05-13 | 株式会社東海ヒット | Specimen temperature controller for microscope |
| JP2004177846A (en) * | 2002-11-29 | 2004-06-24 | Tokai Hit:Kk | Transparent heating device for microscopic observation |
| JP2006025612A (en) * | 2004-07-12 | 2006-02-02 | Nikon Corp | Pyrostat |
| WO2009041200A1 (en) * | 2007-09-27 | 2009-04-02 | Tokai Hit Co., Ltd. | Sample stage device having temperature gradient |
| CN201454570U (en) * | 2009-09-10 | 2010-05-12 | 王敏康 | Large-plane temperature control heating table |
| CN103861676A (en) * | 2014-03-18 | 2014-06-18 | 青岛汉源微电子有限公司 | Ultra-thin precision temperature control heating platform |
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| CN206834150U (en) * | 2017-05-10 | 2018-01-02 | 南京农业大学 | A kind of microscope thermal station |
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| Publication number | Publication date |
|---|---|
| CN107017144A (en) | 2017-08-04 |
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