CN107017144A - A kind of microscope thermal station - Google Patents
A kind of microscope thermal station Download PDFInfo
- Publication number
- CN107017144A CN107017144A CN201710323744.0A CN201710323744A CN107017144A CN 107017144 A CN107017144 A CN 107017144A CN 201710323744 A CN201710323744 A CN 201710323744A CN 107017144 A CN107017144 A CN 107017144A
- Authority
- CN
- China
- Prior art keywords
- temperature control
- heating
- control case
- bottom plate
- thermal station
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000010438 heat treatment Methods 0.000 claims abstract description 63
- 239000011521 glass Substances 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims description 6
- 229910000838 Al alloy Inorganic materials 0.000 claims description 4
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims description 3
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 4
- 210000000130 stem cell Anatomy 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000000287 oocyte Anatomy 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/02—Details
- H01J37/20—Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support
-
- H—ELECTRICITY
- H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
- H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
- H05B3/00—Ohmic-resistance heating
- H05B3/20—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater
- H05B3/22—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible
- H05B3/28—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible heating conductor embedded in insulating material
- H05B3/30—Heating elements having extended surface area substantially in a two-dimensional plane, e.g. plate-heater non-flexible heating conductor embedded in insulating material on or between metallic plates
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of microscope thermal station, including temperature control case and warm table, AC/DC power supplys and temperature controller are provided with the temperature control case, the rear end of the temperature control case is provided with power outlet, front end is provided with heating socket and switch, and the front end of the temperature controller is the temperature control panel for protruding from temperature control case front end face；The warm table includes folding bottom plate, soft adiabator layer, heating film and the heating platform set successively from the bottom up, the lower section of the bottom plate is provided with support feet, the heating film is connected by heating cable with the heating socket of temperature control case, the warm table is provided with sight vane, the sight vane and is embedded with combining glass.The present invention can meet embryo or egg mother cell steady temperature operation needs, can there is enough operating platforms again, and convenient operation is durable, easy to carry.
Description
Technical field
The invention belongs to electron microscope accessory technical field, and in particular to a kind of microscope thermal station.
Background technology
The manipulation in vitro of external egg mother cell, embryo and some special cells is required for operating at a suitable temperature,
Particularly oocyte maturation early stage picks up ovum process, the stoning of body-cell neucleus transplanting, with during Somatic Fusion etc., all needing
Will be at a suitable temperature（37-38.5℃）Operation.Thermal station is carried because Stereo microscope is typically no, it is suitable in order to obtain
Operation temperature, then need to make suitable thermal station to measure for Stereo microscope.This thermal station should can guarantee that stationary temperature, again
Ensure not influence observing effect under Stereo microscope, not only to meet operating table surface width when using, while can be adapted to again
Microscopical base.Although the thermal station now with some imports can meet above demand, the specification of these thermal station is all special
It is fixed, it can be positioned over just under the microscope of certain model, their whole operation table top is narrower, it is impossible to meet somatic cell nuclear shifting
During plant, particularly handmade cloning when operational requirements.In addition, some microscope hot stages are then glass materials, although to microscope
Lower observation is more convenient, but its material is frangible, it is easy to by " experiment new hand " destruction.Therefore in the urgent need to producing a kind of microscope
Thermal station, broader operating table surface can be met simultaneously and firmly solid, and can keep accurate constant temperature.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of microscope thermal station, with the accurate and constant operation of holding
Temperature, broad comfortable operating table surface is durable, easy to carry.
In order to solve the above technical problems, embodiments of the invention provide a kind of microscope thermal station, including temperature control case and
AC/DC power supplys and temperature controller are provided with warm table, the temperature control case, the rear end of the temperature control case is provided with power outlet, preceding
End is provided with heating socket and switch, and the front end of the temperature controller is the temperature control panel for protruding from temperature control case front end face；
The warm table includes folding bottom plate, soft adiabator layer, heating film and the heating platform set successively from the bottom up, described
The lower section of bottom plate is provided with support feet, and the heating film is connected by heating cable with the heating socket of temperature control case, the heating
Platform is provided with sight vane, the sight vane and is embedded with combining glass.
Wherein, the front end of the temperature control case is additionally provided with temperature sensor socket, and the lower end of the heating platform is provided with recessed
Temperature sensor is equipped with groove, the groove, the temperature sensor passes through sensor wire and the TEMP of temperature control case
Device socket is connected.
Further, cabling channel is offered on the lower surface of the heating platform, the sensor wire is arranged in cabling channel
It is interior.
Above-mentioned microscope thermal station also includes fixed frame, and the upper end of the fixed frame is fixed with heating platform to be connected
Connect, lower end is fixedly connected with bottom plate.
It is preferred that, the fixed frame is formed by four structure side slats are end to end, the upper end of each structure side slat
Face is fixedly connected with the lower surface of heating platform by screw, and the lower surface of each structure side slat and the upper surface of bottom plate are logical
Cross screw to be fixedly connected, the heating film and soft adiabator layer between the heating platform and bottom plate are enclosed located at fixed frame
In frame.
Wherein, provided with the glass seal being fixedly connected with bottom plate lower surface below the sight vane, the glass seal
In upper end insertion sight vane, the combining glass is located at top, the inside of sight vane of glass seal.
It is preferred that, the support feet is telescopic support pin, can be adjusted according to Microscopii height or experiment demand
Section height.
It is preferred that, the material that the heating platform and bottom plate are used is aluminium alloy 6063, and the soft adiabator layer is
Alumina silicate fibre layer.The combining glass selects high printing opacity heat-resistant, high strength degree glass.
The above-mentioned technical proposal of the present invention has the beneficial effect that：The present invention can meet embryo or egg mother cell constant temperature
Degree operation needs, and can have enough operating platforms again, and convenient operation is durable, easy to carry.
Brief description of the drawings
Fig. 1 is the structural representation of the embodiment of the present invention one；
Fig. 2 is the structure decomposition figure of temperature control case in embodiment one；
Fig. 3 is the structure decomposition figure of embodiment one；
Fig. 4 is the top view of warm table in embodiment one；
Fig. 5 be Fig. 4 in along D-D sectional view；
Fig. 6 is the partial enlarged drawing at A in Fig. 5；
Fig. 7 is the control flow block diagram of the embodiment of the present invention one.
Description of reference numerals：
1st, temperature control case；100th, AC/DC power supplys；101st, temperature controller；102nd, power outlet；103rd, socket is heated；104th, temperature is passed
Sensor socket；105th, switch；106th, temperature control panel；2nd, warm table；200th, bottom plate；201st, soft adiabator layer；202nd, heat
Film；203rd, heating platform；204th, support feet；205th, sight vane；206th, combining glass；207th, glass seal；3rd, heating cable；4、
Temperature sensor；5th, sensor wire；6th, structure side slat.
Embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing and tool
Body embodiment is described in detail.
A kind of microscope thermal station, including temperature control case 1, warm table 2 and connecting line, its control flow block diagram such as Fig. 7 institutes
Show.
As shown in Figure 1 and Figure 2, AC/DC power supplys 100 and temperature controller 101, the temperature control case are provided with the temperature control case 1
1 rear end is provided with power outlet 102, and front end is provided with heating socket 103, temperature sensor socket 104 and switch 105, the temperature
The front end for controlling device 101 is the temperature control panel 106 for protruding from the front end face of temperature control case 1.It is therein to have AC/DC power supplys 100 be in electricity
Keep persistently inputting the 220V use for laboratory power supply of AC/DC power supplys 100 under the connection of supply socket 102, and pass through AC/DC power supplys
100 conversion exports the 24V dc sources needed to heating film 202.Temperature controller 101 is commercially available commercially available temperature control instrument, including
Temperature measuring device, humidostat, workable air temperature system etc..
As shown in Fig. 3, Fig. 5, the warm table 2 includes folding bottom plate 200, the soft adiabator layer set successively from the bottom up
201st, heating film 202 and heating platform 203, the lower section of the bottom plate 200 are provided with support feet 204, and the heating film 202 is by adding
Hot line cable 3 is connected with the heating socket 103 of temperature control case 1, and the warm table 2 is provided with circular sight vane 205（See Fig. 4）,
Position of the sight vane 205 on warm table is set according to be applicable microscope, and perspective glass is embedded with the sight vane 205
Glass 206.
The material that above-mentioned heating platform and bottom plate is used is aluminium alloy 6063, and light weight easy processing, heat conductivility is excellent.It is soft
Property adiabator layer be alumina silicate fibre layer, good heat insulating is soft, compressible, causes heating film to be fitted tightly with heating platform
It is close.
In the present embodiment, the lower section of sight vane 205 is provided with the glass seal 207 being fixedly connected with the lower surface of bottom plate 200,
The glass seal 207 upper end insertion sight vane 205 in, the combining glass 206 located at glass seal 207 top, thoroughly
The inside of window 205.The combining glass 206 selects high printing opacity heat-resistant, high strength degree glass.
As shown in fig. 6, the lower end of the heating platform 203, the position of neighbouring sight vane 205 are provided with groove, the groove
Temperature sensor 4 is equipped with, temp probe is which is provided with, the temperature sensor 4 passes through sensor wire 5 and temperature control case 1
Temperature sensor socket 104 be connected, can accurate real-time temperature control, error is in the range of ± 0.5 oC.
As shown in figure 5, offering cabling channel on the lower surface of the heating platform 203, the sensor wire 5 is arranged in
In cabling channel.
Above-mentioned microscope also includes fixed frame with thermal station, and upper end and the heating platform 203 of the fixed frame are fixed
Connection, lower end is fixedly connected with bottom plate 200, and position corresponding with cabling channel has what is passed through for sensor wire 5 on fixed frame
Through hole.
As shown in figure 3, the fixed frame is formed by four structure side slats 6 are end to end, on each structure side slat 6 every
5cm is screwed hole, and the upper surface of each structure side slat 6 is fixedly connected with the lower surface of heating platform 203 by screw, often
The lower surface of structure side slat 6 is fixedly connected with the upper surface of bottom plate 200 by screw described in one, positioned at the heating platform 203
Heating film 202 and soft adiabator layer 201 between bottom plate 200 are enclosed in fixed frame, and structure side slat and heating
Connection between platform and bottom plate, soft adiabator layer and heating film are fitted tightly on the bottom surface of heating platform, so that
Obtain good heating properties.
The fixed frame can also use following structures：Formed by four structure side slats are end to end, the structure side
The inner side of bar is provided with neck.Screwed hole, the upper surface of each structure side slat and heating every 5cm on each structure side slat
The lower surface of platform is fixedly connected by screw, and the lower surface of each structure side slat and the upper surface of bottom plate are solid by screw
Fixed connection, the side of heating film and soft adiabator layer between the heating platform and bottom plate is arranged in neck.
The support feet 204 is telescopic support pin, can be high according to Microscopii height or experiment demand regulation
Degree.
It is adjustable that the present embodiment may be designed to link position of the support feet on bottom plate lower surface, such as the lower surface in bottom plate
The upper multigroup screwed hole group of design, support feet realizes that position is adjustable by being connected from different screwed hole groups；Can also be under bottom plate
Horizontal and/or vertical chute is offered on surface, support feet slides to fixed after correct position in chute.
The embodiment of the present invention also provides a kind of electron microscope using above-mentioned microscope thermal station, includes but is not limited to
Stereo microscope, the Stereo microscope is provided with objective table, object lens and light source, and the microscope hot stage is on objective table
Side, the sight vane is located between object lens and light source, and observing object is located between sight vane and object lens.
The present invention is to provide a kind of portable microscope thermal station, including warm table, controlling machine box and controlling machine box with
Connection cables between warm table, warm table is mainly by the panel of the material of two panels aluminium alloy 6063（Heating platform and bottom plate）Centre folder
Heating film and soft insulation material is constituted, and the centre position fitted in heating platform with heating film sets temperature sensor to visit
Head and sensor wire, so as to the more accurate temperature control panel for obtaining the temperature of heating platform and passing to controlling machine box, enter one
Step controls the temperature of heating platform constant in real time by AC/DC power supplys output electric energy.And AC/DC power supplys can be in input 220V laboratories
With 24V dc sources are exported in the case of power supply, so that safer.
The warm table size of the present invention not only can need design size according to the size and actual use of Microscope base,
And perspective hole site can also change according to the need for microscope light source position.Therefore, described microscope is with thermal station
Embryo or egg mother cell steady temperature operation needs can be met, such as pick up ovum process, body cell in oocyte maturation early stage
38.5o constant temperature can be provided in the operation such as stoning of nuclear transfer, can there is enough operating platforms, convenient operation again.
Described above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, on the premise of principle of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (9)
1. AC/ is provided with a kind of microscope thermal station, it is characterised in that including temperature control case and warm table, the temperature control case
D/C power and temperature controller, the rear end of the temperature control case are provided with power outlet, and front end is provided with heating socket and switch, the temperature control
The front end of device is the temperature control panel for protruding from temperature control case front end face；
The warm table includes folding bottom plate, adiabator layer, heating film and the heating platform set, the bottom plate successively from the bottom up
Lower section be provided with support feet, the heating film is connected by heating cable with the heating socket of temperature control case, on the warm table
Provided with sight vane, combining glass is embedded with the sight vane.
2. microscope thermal station according to claim 1, it is characterised in that the front end of the temperature control case is additionally provided with temperature
Sensor socket, the lower end of the heating platform, which is provided with groove, the groove, is equipped with temperature sensor, the temperature sensor
It is connected by sensor wire with the temperature sensor socket of temperature control case.
3. microscope thermal station according to claim 2, it is characterised in that offered on the lower surface of the heating platform
Cabling channel, the sensor wire is arranged in cabling channel.
4. microscope thermal station according to claim 1, it is characterised in that also including fixed frame, the fixed frame
Upper end be fixedly connected with heating platform, lower end is fixedly connected with bottom plate.
5. microscope thermal station according to claim 4, it is characterised in that the fixed frame is first by four structure side slats
Tail, which connects, to be formed, and the upper surface of each structure side slat is fixedly connected with the lower surface of heating platform by screw, Mei Yisuo
State the lower surface of structure side slat to be fixedly connected by screw with the upper surface of bottom plate, between the heating platform and bottom plate
Heating film and soft adiabator layer are enclosed in fixed frame.
6. microscope thermal station according to claim 1, it is characterised in that be provided with and bottom plate following table below the sight vane
In the glass seal that face is fixedly connected, the upper end insertion sight vane of the glass seal, the combining glass is located at glass seal
Top, the inside of sight vane.
7. microscope thermal station according to claim 1, it is characterised in that the support feet is telescopic support pin.
8. microscope thermal station according to claim 1, it is characterised in that the material that the heating platform and bottom plate are used
For aluminium alloy 6063, the soft adiabator layer is alumina silicate fibre layer.
9. the microscope thermal station according to claim 1 or 6, it is characterised in that the combining glass is resistance to from high printing opacity
Warm high strength glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323744.0A CN107017144B (en) | 2017-05-10 | 2017-05-10 | A kind of microscope thermal station |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323744.0A CN107017144B (en) | 2017-05-10 | 2017-05-10 | A kind of microscope thermal station |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107017144A true CN107017144A (en) | 2017-08-04 |
| CN107017144B CN107017144B (en) | 2019-07-09 |
Family
ID=59449780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710323744.0A Expired - Fee Related CN107017144B (en) | 2017-05-10 | 2017-05-10 | A kind of microscope thermal station |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107017144B (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2051746U (en) * | 1989-04-12 | 1990-01-24 | 山东省济南卫生学校 | Warm keeping apparatus for microscopic observation of biological alive specimen |
| JPH0921960A (en) * | 1995-07-05 | 1997-01-21 | Olympus Optical Co Ltd | Heat insulating device for microscope |
| JPH1138329A (en) * | 1997-07-22 | 1999-02-12 | Tokai Hit:Kk | Specimen temperature controller for microscope |
| US20030113905A1 (en) * | 2001-12-19 | 2003-06-19 | Chung-Liang Ho | Culturing chamber on microscope stage |
| JP2004177846A (en) * | 2002-11-29 | 2004-06-24 | Tokai Hit:Kk | Transparent heating device for microscopic observation |
| JP2006025612A (en) * | 2004-07-12 | 2006-02-02 | Nikon Corp | Pyrostat |
| WO2009041200A1 (en) * | 2007-09-27 | 2009-04-02 | Tokai Hit Co., Ltd. | Sample stage device having temperature gradient |
| JP4262390B2 (en) * | 2000-06-06 | 2009-05-13 | 株式会社東海ヒット | Specimen temperature controller for microscope |
| CN201454570U (en) * | 2009-09-10 | 2010-05-12 | 王敏康 | Large-plane temperature control heating table |
| CN103861676A (en) * | 2014-03-18 | 2014-06-18 | 青岛汉源微电子有限公司 | Ultra-thin precision temperature control heating platform |
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| CN206834150U (en) * | 2017-05-10 | 2018-01-02 | 南京农业大学 | A kind of microscope thermal station |
-
2017
- 2017-05-10 CN CN201710323744.0A patent/CN107017144B/en not_active Expired - Fee Related
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2051746U (en) * | 1989-04-12 | 1990-01-24 | 山东省济南卫生学校 | Warm keeping apparatus for microscopic observation of biological alive specimen |
| JPH0921960A (en) * | 1995-07-05 | 1997-01-21 | Olympus Optical Co Ltd | Heat insulating device for microscope |
| JPH1138329A (en) * | 1997-07-22 | 1999-02-12 | Tokai Hit:Kk | Specimen temperature controller for microscope |
| JP4262390B2 (en) * | 2000-06-06 | 2009-05-13 | 株式会社東海ヒット | Specimen temperature controller for microscope |
| US20030113905A1 (en) * | 2001-12-19 | 2003-06-19 | Chung-Liang Ho | Culturing chamber on microscope stage |
| JP2004177846A (en) * | 2002-11-29 | 2004-06-24 | Tokai Hit:Kk | Transparent heating device for microscopic observation |
| JP2006025612A (en) * | 2004-07-12 | 2006-02-02 | Nikon Corp | Pyrostat |
| WO2009041200A1 (en) * | 2007-09-27 | 2009-04-02 | Tokai Hit Co., Ltd. | Sample stage device having temperature gradient |
| CN201454570U (en) * | 2009-09-10 | 2010-05-12 | 王敏康 | Large-plane temperature control heating table |
| CN103861676A (en) * | 2014-03-18 | 2014-06-18 | 青岛汉源微电子有限公司 | Ultra-thin precision temperature control heating platform |
| CN104267766A (en) * | 2014-10-13 | 2015-01-07 | 中国科学技术大学 | Real-time temperature-control micro sample room |
| CN206834150U (en) * | 2017-05-10 | 2018-01-02 | 南京农业大学 | A kind of microscope thermal station |
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| Publication number | Publication date |
|---|---|
| CN107017144B (en) | 2019-07-09 |
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