CN107006375A - A kind of orchid fast propagating culture medium - Google Patents
A kind of orchid fast propagating culture medium Download PDFInfo
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- CN107006375A CN107006375A CN201710406787.5A CN201710406787A CN107006375A CN 107006375 A CN107006375 A CN 107006375A CN 201710406787 A CN201710406787 A CN 201710406787A CN 107006375 A CN107006375 A CN 107006375A
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- culture medium
- orchid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The invention belongs to field of plant tissue culture technique, there is provided a kind of orchid fast propagating culture medium, including primary culture medium, three kinds of culture mediums of proliferated culture medium and root media.A kind of orchid fast propagating culture medium that the present invention is provided is formulated growth coefficient height, fast growth, and tissue-cultured seedling health, strong environmental adaptability, transplanting survival rate are high, meanwhile, the growth and environment to orchid are harmless.
Description
Technical field
The invention belongs to field of plant tissue culture technique, in particular it relates to a kind of orchid fast propagating culture medium.
Background technology
The orchid of Chinese tradition, refers mainly to epidendrum, referred to as China blue, orchid as integrate view and admire with it is medicinal
Plant not only possess vast fan, an and part as traditional Chinese culture.
However, traditional modes of reproduction of orchid is division propagation, breeding coefficient is low, speed is slow, and industrialization level is low, by ground
Environment and the limitation in season are managed, purpose that is quick, efficiently breeding is extremely difficult to, market comsupton demand far can not be met.Pass through
This technological means of tissue cultures and method are the current effective measures for solving orchid breeding, but used culture medium is exhausted
Most of is to add chemical synthesis plant hormone to promote micropropagation of plants and growth, and often resistance is poor for tissue-cultured seedling, transplant
The death rate is higher.
The content of the invention
For defect of the prior art, it is an object of the invention to provide a kind of orchid fast propagating culture medium.
A kind of orchid fast propagating culture medium that the present invention is provided is formulated growth coefficient height, fast growth, and tissue culture
Seedling health, strong environmental adaptability, transplanting survival rate are high, meanwhile, the growth and environment to orchid are harmless.
A kind of orchid fast propagating culture medium provided according to the present invention, the orchid fast propagating culture medium includes primary
Culture medium, three kinds of culture mediums of proliferated culture medium and root media, wherein,
Original cuiture based formulas is：MS, peptone 0.5-1.0g/L, wheat flour 20-35g/L, coconut juice 50-100ml/L, maltose
10-25g/L、KNO31200-1400mg/L、NH4Cl100-350mg/L、KH2PO4110-130mg/L、MgSO4·7H2O230-
200mg/L、CaCl2200-240mg/L, pH value is 5.5-6.1；
Proliferation culture medium formula is：1/2MS, 6-BA0.5-2mg/L, KT0.05-0.1mg/L, peptone 0.1-1.0g/L, Ma Ling
Potato 20-35g/L, banana puree 50-90ml/L, sucrose 10-20g/L, pH value is 5.6-6.3；
Prescription of rooting medium is：1/2MS, cider 30-50g/L, sucrose 10-20g/L, peptone 0.5-1.2g/L, bumps
Rod powder 0.5-1.1g/L, MnSO4·4H2O22.4mg/L、ZnSO4·7H2O8.5mg/L、Na2MoO4·2H2O0.22 mg/L、
CuSO4·5H2O0.022mg/L、CoCl2·6H2O0.022mg/L、Na2·EDTA37.2mg/L、FeSO 4·7H 2
O21.8mg/L、C6H12O6·2H2O90mg/L、NH2·CH 2COOH2mg/L, NAA0.01-0.4 mg/L, pH value are 5.6-
6.8。
Preferably, the coconut juice is to heat coconut water, and temperature is between 60-70 DEG C, with being added to training after filtered through gauze
Support in base.
Preferably, the MS of the primary culture medium is the solid medium that agar is supporter.
Preferably, the 1/2MS of the proliferation culture medium formula is the solid medium that agar is supporter.
Preferably, the 1/2MS of the prescription of rooting medium is the solid medium that agar is supporter.
Preferably, explant used in the original cuiture is cymbidium seed.
Preferably, the cider is to heat apple water, and temperature is between 60-70 DEG C, with being added to after filtered through gauze
In culture medium.
Preferably, the fineness of the attapulgite is 60-100 mesh.
Compared with prior art, the present invention has following beneficial effect：
The present invention is by selecting suitable primary culture medium, proliferated culture medium and root media to have both fluid nutrient medium and solid
The strong point of body culture medium realizes high efficiently multiplying, improves transplanting survival rate, and tissue-cultured seedling is healthy, strong environmental adaptability, furthermore, it is former
Being compared for the more traditional MS culture mediums of its inorganic salt content of culture medium about reduces 5-15%；Its inorganic salt content of proliferated culture medium compared with
Traditional MS culture mediums, which are compared, about reduces 2-8%.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.
A kind of orchid fast propagating culture medium that the present invention is provided is formulated growth coefficient height, fast growth, and tissue culture
Seedling health, strong environmental adaptability, transplanting survival rate are high, meanwhile, the growth and environment to orchid are harmless.
A kind of orchid fast propagating culture medium provided according to the present invention, the orchid fast propagating culture medium includes primary
Culture medium, three kinds of culture mediums of proliferated culture medium and root media, wherein,
Original cuiture based formulas is：MS, peptone 0.5-1.0g/L, wheat flour 20-35g/L, coconut juice 50-100ml/L, maltose
10-25g/L、KNO31200-1400mg/L、NH4Cl100-350mg/L、KH2PO4110-130mg/L、MgSO4·7H2O230-
200mg/L、CaCl2200-240mg/L, pH value is 5.5-6.1；
Proliferation culture medium formula is：1/2MS, 6-BA0.5-2mg/L, KT0.05-0.1mg/L, peptone 0.1-1.0g/L, Ma Ling
Potato 20-35g/L, banana puree 50-90ml/L, sucrose 10-20g/L, pH value is 5.6-6.3；
Prescription of rooting medium is：1/2MS, cider 30-50g/L, sucrose 10-20g/L, peptone 0.5-1.2g/L, bumps
Rod powder 0.5-1.1g/L, MnSO4·4H2O22.4mg/L、ZnSO4·7H2O8.5mg/L、Na2MoO4·2H2O0.22 mg/L、
CuSO4·5H2O0.022mg/L、CoCl2·6H2O0.022mg/L、Na2·EDTA37.2mg/L、FeSO 4·7H 2
O21.8mg/L、C6H12O6·2H2O90mg/L、NH2·CH 2COOH2mg/L, NAA0.01-0.4 mg/L, pH value are 5.6-
6.8。
Preferably, the coconut juice is to heat coconut water, and temperature is between 60-70 DEG C, with being added to training after filtered through gauze
Support in base.
Preferably, the MS of the primary culture medium is the solid medium that agar is supporter.
Preferably, the 1/2MS of the proliferation culture medium formula is the solid medium that agar is supporter.
Preferably, the 1/2MS of the prescription of rooting medium is the solid medium that agar is supporter.
Preferably, explant used in the original cuiture is cymbidium seed.
Preferably, the cider is to heat apple water, and temperature is between 60-70 DEG C, with being added to after filtered through gauze
In culture medium.
Preferably, the fineness of the attapulgite is 60-100 mesh.
Compared with prior art, the present invention has following beneficial effect：
The present invention is by selecting suitable primary culture medium, proliferated culture medium and root media to have both fluid nutrient medium and solid
The strong point of body culture medium realizes high efficiently multiplying, improves transplanting survival rate, and tissue-cultured seedling is healthy, strong environmental adaptability, furthermore, it is former
Being compared for the more traditional MS culture mediums of its inorganic salt content of culture medium about reduces 5-15%；Its inorganic salt content of proliferated culture medium compared with
Traditional MS culture mediums, which are compared, about reduces 2-8%.
Embodiment 1
A kind of orchid fast propagating culture medium that the present embodiment is provided, the orchid fast propagating culture medium includes original cuiture
Base, three kinds of culture mediums of proliferated culture medium and root media, wherein,
Original cuiture based formulas is：MS, peptone 1.0g/L, wheat flour 20g/L, coconut juice 100ml/L, maltose 10g/L,
KNO31400mg/L、NH4Cl100mg/L、KH2PO4130mg/L、MgSO4·7H2O230mg/L、CaCl2200-240mg/L, pH value
For 6.1；
Proliferation culture medium formula is：1/2MS, 6-BA2mg/L, KT0.05mg/L, peptone 1.0g/L, potato 20g/L, banana
Mud 90ml/L, sucrose 10g/L, pH value is 6.3；
Prescription of rooting medium is：1/2MS, cider 50g/L, sucrose 10g/L, peptone 1.2g/L, attapulgite 0.5g/L,
MnSO4·4H2O22.4mg/L、ZnSO4·7H2O8.5mg/L、Na2MoO4·2H2O0.22 mg/L、CuSO4·
5H2O0.022mg/L、CoCl2·6H2O0.022mg/L、Na2·EDTA37.2mg/L、FeSO 4·7H 2 O21.8mg/L、
C6H12O6·2H2O90mg/L、NH2·CH 2COOH2mg/L, NAA0.4 mg/L, pH value are 6.8.
The coconut juice is to heat coconut water, and temperature is between 70 DEG C, with being added to after filtered through gauze in culture medium.
The MS of the primary culture medium is the solid medium that agar is supporter.
The 1/2MS of the proliferation culture medium formula is the solid medium that agar is supporter.
The 1/2MS of the prescription of rooting medium is the solid medium that agar is supporter.
Explant used in the original cuiture is cymbidium seed.
The cider is to heat apple water, and temperature is at 60 DEG C, with being added to after filtered through gauze in culture medium.
The fineness of the attapulgite is 100 mesh.
Embodiment 2
A kind of orchid fast propagating culture medium that the present embodiment is provided, the orchid fast propagating culture medium includes original cuiture
Base, three kinds of culture mediums of proliferated culture medium and root media, wherein,
Original cuiture based formulas is：MS, peptone 0.5g/L, wheat flour 35g/L, coconut juice 50ml/L, maltose 25g/L,
KNO31200mg/L、NH4Cl350mg/L、KH2PO4110mg/L、MgSO4·7H2O2200mg/L、CaCl2200mg/L, pH value is
6.1；
Proliferation culture medium formula is：1/2MS, 6-BA0.5mg/L, KT0.1mg/L, peptone 0.1g/L, potato 35g/L, perfume (or spice)
Any of several broadleaf plants mud 50ml/L, sucrose 20g/L, pH value is 5.6；
Prescription of rooting medium is：1/2MS, cider 30g/L, sucrose 20g/L, peptone 0.5g/L, attapulgite 1.1g/L,
MnSO4·4H2O22.4mg/L、ZnSO4·7H2O8.5mg/L、Na2MoO4·2H2O0.22 mg/L、CuSO4·
5H2O0.022mg/L、CoCl2·6H2O0.022mg/L、Na2·EDTA37.2mg/L、FeSO 4·7H 2 O21.8mg/L、
C6H12O6·2H2O90mg/L、NH2·CH 2COOH2mg/L, NAA0.01mg/L, pH value are 5.6.
The coconut juice is to heat coconut water, and temperature is at 60 DEG C, with being added to after filtered through gauze in culture medium.
The MS of the primary culture medium is the solid medium that agar is supporter.
The 1/2MS of the proliferation culture medium formula is the solid medium that agar is supporter.
The 1/2MS of the prescription of rooting medium is the solid medium that agar is supporter.
Explant used in the original cuiture is cymbidium seed.
The cider is to heat apple water, and temperature is at 70 DEG C, with being added to after filtered through gauze in culture medium.
The fineness of the attapulgite is 60 mesh.
Embodiment 3
A kind of orchid fast propagating culture medium that the present embodiment is provided, the orchid fast propagating culture medium includes original cuiture
Base, three kinds of culture mediums of proliferated culture medium and root media, wherein,
Original cuiture based formulas is：MS, peptone 0.7g/L, wheat flour 29g/L, coconut juice 80ml/L, maltose 15g/L,
KNO31300mg/L、NH4Cl250mg/L、KH2PO4115mg/L、MgSO4·7H2O260mg/L、CaCl2220mg/L, pH value is
5.8；
Proliferation culture medium formula is：1/2MS, 6-BA0.9mg/L, KT0.07mg/L, peptone 0.6g/L, potato 29g/L, perfume (or spice)
Any of several broadleaf plants mud 57ml/L, sucrose 14g/L, pH value is 5.9；
Prescription of rooting medium is：1/2MS, cider 40g/L, sucrose 18g/L, peptone 0.7g/L, attapulgite 0.6g/L,
MnSO4·4H2O22.4mg/L、ZnSO4·7H2O8.5mg/L、Na2MoO4·2H2O0.22 mg/L、CuSO4·
5H2O0.022mg/L、CoCl2·6H2O0.022mg/L、Na2·EDTA37.2mg/L、FeSO 4·7H 2 O21.8mg/L、
C6H12O6·2H2O90mg/L、NH2·CH 2COOH2mg/L, NAA0.06 mg/L, pH value are 5.6-6.8.
The coconut juice is to heat coconut water, and temperature is between 66 DEG C, with being added to after filtered through gauze in culture medium.
The MS of the primary culture medium is the solid medium that agar is supporter.
The 1/2MS of the proliferation culture medium formula is the solid medium that agar is supporter.
The 1/2MS of the prescription of rooting medium is the solid medium that agar is supporter.
Explant used in the original cuiture is cymbidium seed.
The cider is to heat apple water, and temperature is at 66 DEG C, with being added to after filtered through gauze in culture medium.
The fineness of the attapulgite is 70 mesh.
Embodiment 4
A kind of orchid fast propagating culture medium that the present embodiment is provided, the orchid fast propagating culture medium includes original cuiture
Base, three kinds of culture mediums of proliferated culture medium and root media, wherein,
Original cuiture based formulas is：MS, peptone 0.7g/L, wheat flour 23g/L, coconut juice 80ml/L, maltose 17g/L,
KNO31250mg/L、NH4Cl180mg/L、KH2PO4125mg/L、MgSO4·7H2O280mg/L、CaCl2230mg/L, pH value is
5.9；
Proliferation culture medium formula is：1/2MS, 6-BA0.9mg/L, KT0.07mg/L, peptone 0.4g/L, potato 23g/L, perfume (or spice)
Any of several broadleaf plants mud 70ml/L, sucrose 17g/L, pH value is 5.8；
Prescription of rooting medium is：1/2MS, cider 35g/L, sucrose 16g/L, peptone 0.7g/L, attapulgite 0.7g/L,
MnSO4·4H2O22.4mg/L、ZnSO4·7H2O8.5mg/L、Na2MoO4·2H2O0.22 mg/L、CuSO4·
5H2O0.022mg/L、CoCl2·6H2O0.022mg/L、Na2·EDTA37.2mg/L、FeSO 4·7H 2 O21.8mg/L、
C6H12O6·2H2O90mg/L、NH2·CH 2COOH2mg/L, NAA0.01-0.4 mg/L, pH value are 5.6-6.8.
The coconut juice is to heat coconut water, and temperature is at 63 DEG C, with being added to after filtered through gauze in culture medium.
The MS of the primary culture medium is the solid medium that agar is supporter.
The 1/2MS of the proliferation culture medium formula is the solid medium that agar is supporter.
The 1/2MS of the prescription of rooting medium is the solid medium that agar is supporter.
Explant used in the original cuiture is cymbidium seed.
The cider is to heat apple water, and temperature is at 65 DEG C, with being added to after filtered through gauze in culture medium.
The fineness of the attapulgite is 90 mesh.
The specific embodiment of the present invention is described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring the substantive content of the present invention.
Claims (8)
1. a kind of orchid fast propagating culture medium, it is characterised in that
The orchid fast propagating culture medium includes primary culture medium, three kinds of culture mediums of proliferated culture medium and root media, its
In,
Original cuiture based formulas is：MS, peptone 0.5-1.0g/L, wheat flour 20-35g/L, coconut juice 50-100ml/L, maltose
10-25g/L、KNO31200-1400mg/L、NH4Cl100-350mg/L、KH2PO4110-130mg/L、MgSO4·7H2O230-
200mg/L、CaCl2200-240mg/L, pH value is 5.5-6.1；
Proliferation culture medium formula is：1/2MS, 6-BA0.5-2mg/L, KT0.05-0.1mg/L, peptone 0.1-1.0g/L, Ma Ling
Potato 20-35g/L, banana puree 50-90ml/L, sucrose 10-20g/L, pH value is 5.6-6.3；
Prescription of rooting medium is：1/2MS, cider 30-50g/L, sucrose 10-20g/L, peptone 0.5-1.2g/L, bumps
Rod powder 0.5-1.1g/L, MnSO4·4H2O22.4mg/L、ZnSO4·7H2O8.5mg/L、Na2MoO4·2H2O0.22 mg/L、
CuSO4·5H2O0.022mg/L、CoCl2·6H2O0.022mg/L、Na2·EDTA37.2mg/L、FeSO 4·7H 2
O21.8mg/L、C6H12O6·2H2O90mg/L、NH2·CH 2COOH2mg/L, NAA0.01-0.4 mg/L, pH value are 5.6-
6.8。
2. orchid fast propagating culture medium according to claim 1, it is characterised in that：The coconut juice is to add coconut water
Heat, temperature is between 60-70 DEG C, with being added to after filtered through gauze in culture medium.
3. orchid fast propagating culture medium according to claim 1, it is characterised in that：The MS of the primary culture medium is fine jade
Fat is the solid medium of supporter.
4. orchid fast propagating culture medium according to claim 1, it is characterised in that：The 1/ of the proliferation culture medium formula
2MS is the solid medium that agar is supporter.
5. orchid fast propagating culture medium according to claim 1, it is characterised in that：The 1/ of the prescription of rooting medium
2MS is the solid medium that agar is supporter.
6. orchid fast propagating culture medium according to claim 1, it is characterised in that：It is outer used in the original cuiture
Implant is cymbidium seed.
7. orchid fast propagating culture medium according to claim 1, it is characterised in that：The cider is to add apple water
Heat, temperature is between 60-70 DEG C, with being added to after filtered through gauze in culture medium.
8. orchid fast propagating culture medium according to claim 1, it is characterised in that：The fineness of the attapulgite is
60-100 mesh.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110663547A (en) * | 2018-07-03 | 2020-01-10 | 中国科学院上海生命科学研究院 | A kind of keel masseus fir explant culture method |
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