CN107002142B - 用于检测含有mecC的甲氧西林抗性的金黄色葡萄球菌的组合物和方法 - Google Patents

用于检测含有mecC的甲氧西林抗性的金黄色葡萄球菌的组合物和方法 Download PDF

Info

Publication number
CN107002142B
CN107002142B CN201580064617.2A CN201580064617A CN107002142B CN 107002142 B CN107002142 B CN 107002142B CN 201580064617 A CN201580064617 A CN 201580064617A CN 107002142 B CN107002142 B CN 107002142B
Authority
CN
China
Prior art keywords
mec
mrsa
oligonucleotide
seq
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580064617.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN107002142A (zh
Inventor
J.A.约翰逊
A.海斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN107002142A publication Critical patent/CN107002142A/zh
Application granted granted Critical
Publication of CN107002142B publication Critical patent/CN107002142B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580064617.2A 2014-12-02 2015-12-01 用于检测含有mecC的甲氧西林抗性的金黄色葡萄球菌的组合物和方法 Active CN107002142B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/558,220 US9920381B2 (en) 2014-12-02 2014-12-02 Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
US14/558220 2014-12-02
PCT/EP2015/078201 WO2016087438A1 (en) 2014-12-02 2015-12-01 Compositions and methods for detecting mecc containing methicillin-resistant staphylococcus aureus

Publications (2)

Publication Number Publication Date
CN107002142A CN107002142A (zh) 2017-08-01
CN107002142B true CN107002142B (zh) 2021-04-02

Family

ID=54838318

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580064617.2A Active CN107002142B (zh) 2014-12-02 2015-12-01 用于检测含有mecC的甲氧西林抗性的金黄色葡萄球菌的组合物和方法

Country Status (7)

Country Link
US (2) US9920381B2 (https=)
EP (1) EP3227459B1 (https=)
JP (1) JP6737785B2 (https=)
CN (1) CN107002142B (https=)
CA (1) CA2969580C (https=)
ES (1) ES2729304T3 (https=)
WO (1) WO2016087438A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3251332B1 (en) 2015-01-27 2021-07-07 Healthy.IO Ltd Measuring and monitoring skin feature colors, form and size
ES2991814T3 (es) 2019-12-27 2024-12-05 Hoffmann La Roche Composiciones y procedimientos para detectar Staphylococcus aureus resistente a la meticilina
EP4437142A1 (en) 2021-11-22 2024-10-02 F. Hoffmann-La Roche AG Compositions and methods for detecting vana and/or vanb genes associated with multidrug resistance

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066504A1 (en) * 2009-11-30 2011-06-03 Physicians Reference Laboratory, Llc Methods and compositions for detecting methicillin-resistant staphylococcus aureus
US20110312876A1 (en) * 2010-01-13 2011-12-22 Medical Diagnostic Laboratories, Llc Method of determining types I, II, III, IV or V or methicillin-resistant staphylococcus aureus (MRSA) in a biological sample
WO2012159768A1 (en) * 2011-05-26 2012-11-29 Roche Diagnostics Gmbh Compositions and methods for detection of staphylococcus aureus
WO2013048583A2 (en) * 2011-05-24 2013-04-04 Elitech Holding B.V. Detection of methicillin-resistant staphylococcus aureus
WO2013150376A1 (en) * 2012-04-06 2013-10-10 Geneohm Sciences Canada, Inc. Sequences for detection and identification of methicillin-resistant staphylococcus aureus (mrsa) of mrej type xxi

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0135587B2 (en) 1983-02-22 2002-12-18 Syngene, Inc. Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4996143A (en) 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5683896A (en) 1989-06-01 1997-11-04 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP1067134B1 (en) 1991-11-07 2004-07-28 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
JP4281877B2 (ja) 1996-06-04 2009-06-17 ユニバーシティ オブ ユタ リサーチ ファウンデーション 生物学的プロセスを実行し且つモニタリングするためのシステムと方法
ATE280177T1 (de) 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
CA2359039A1 (en) * 1999-01-29 2000-08-03 Toshio Miyata Meg-4 protein
WO2007039301A2 (en) 2005-10-05 2007-04-12 Roche Diagnostics Gmbh Non-fluorescent energy transfer
US8535888B2 (en) * 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
KR101254881B1 (ko) * 2011-04-07 2013-04-15 로베르트 보쉬 게엠베하 에너지 발생부를 구비한 배터리 팩
IN2014DN05831A (https=) * 2011-12-23 2015-06-26 Biomerieux Sa

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066504A1 (en) * 2009-11-30 2011-06-03 Physicians Reference Laboratory, Llc Methods and compositions for detecting methicillin-resistant staphylococcus aureus
US20110312876A1 (en) * 2010-01-13 2011-12-22 Medical Diagnostic Laboratories, Llc Method of determining types I, II, III, IV or V or methicillin-resistant staphylococcus aureus (MRSA) in a biological sample
WO2013048583A2 (en) * 2011-05-24 2013-04-04 Elitech Holding B.V. Detection of methicillin-resistant staphylococcus aureus
WO2012159768A1 (en) * 2011-05-26 2012-11-29 Roche Diagnostics Gmbh Compositions and methods for detection of staphylococcus aureus
CN103547685A (zh) * 2011-05-26 2014-01-29 霍夫曼-拉罗奇有限公司 用于检测金黄色葡萄球菌的组合物和方法
WO2013150376A1 (en) * 2012-04-06 2013-10-10 Geneohm Sciences Canada, Inc. Sequences for detection and identification of methicillin-resistant staphylococcus aureus (mrsa) of mrej type xxi

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Multiplex Real-Time PCR Assay for Detection of Methicillin-Resistant Staphylococcusaureus (MRSA) Strains Suitable in Regions of High MRSA Endemicity;Jeong-Uk Kim，et al;《Journal of Clinical Microbiology》;20130331;第51卷(第3期);第1008-1013页 *
New Real-Time PCR Assay for Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly fromS pecimens Containing a Mixture of Staphylococci;A.Huletsky，et al;《JOURNALOF CLINICAL MICROBIOLOGY》;20040531;第42卷(第5期);第1875-1884页 *

Also Published As

Publication number Publication date
EP3227459B1 (en) 2019-04-03
JP2018503363A (ja) 2018-02-08
CA2969580C (en) 2023-03-14
US9920381B2 (en) 2018-03-20
US20180148771A1 (en) 2018-05-31
CA2969580A1 (en) 2016-06-09
US10190178B2 (en) 2019-01-29
EP3227459A1 (en) 2017-10-11
JP6737785B2 (ja) 2020-08-12
ES2729304T3 (es) 2019-10-31
US20160153028A1 (en) 2016-06-02
CN107002142A (zh) 2017-08-01
WO2016087438A1 (en) 2016-06-09

Similar Documents

Publication Publication Date Title
US20230056677A1 (en) Compositions and methods for detecting methicillin-resistant staphylococcus aureus
JP7716408B2 (ja) メチシリン耐性黄色ブドウ球菌を検出するための組成物および方法
US9816143B2 (en) Compositions and methods for detection of Staphylococcus aureus
JP6702723B2 (ja) クロストリジウム−ディフィシルの検出のための組成物及び方法
CN107002142B (zh) 用于检测含有mecC的甲氧西林抗性的金黄色葡萄球菌的组合物和方法
JP2018503363A5 (https=)
JP2024539474A (ja) 多剤耐性に関連付けられるｖａｎＡ及び／又はｖａｎＢ遺伝子を検出するための組成物及び方法
CN117858960A (zh) 用于通过双靶标测定来检测丁型肝炎病毒的组合物和方法
CN107922980B (zh) 用于检测结核分枝杆菌的组合物和方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant