CN106993535B - Two leaf pockets are quickly bred by blue method by blue petal piece using two leaf pockets - Google Patents
Two leaf pockets are quickly bred by blue method by blue petal piece using two leaf pockets Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The present invention relates to orchid reproduction technique fields, and in particular to quickly breeds two leaf pockets by blue method by blue petal piece using two leaf pockets.The present invention quickly breeds two leaf pockets by blue petal piece using two leaf pockets and obtains a large amount of adventitious bud, differentiation culture, culture of rootage, hardening and test tube seedling transplant step by blue method, including preparation vegetative propagation petal piece, Fiber differentiation acquisition adventitious bud, Multiplying culture.The present invention chooses two leaf pockets and induces two leaf pockets of quick breeding blue as explant by blue petal piece, by squamous subculture, differentiation culture and culture of rootage, is finally obtained a large amount of high-quality two leaves pockets by blue test tube seedling.Two leaf pockets of method breeding provided by the invention are stablized by blue test tube seedlings, and proliferative speed improves 80% than Traditional breeding processes, and survival rate improves 90%, can solve the two leaf pockets problem long by blue vegetative propagation prior art low reproduction rate, breeding cycle.
Description
Technical field
The invention belongs to orchid reproduction technique fields, and in particular to quickly breed two leaves by blue petal piece using two leaf pockets
Pocket is by blue method.
Background technique
Orchid is graceful unique in the form of it, pattern is gorgeous, color is abundant, the florescence is long, referred to as spend in king.Two leaf pocket quilts
Orchid is even more the fine work of wild orchid.Two leaf pockets are orchid family Neottianthe plant by orchid, and plant is 4-24 centimetres high.Stem tuber spheroidal or
It is oval.Stem is upright or close upright, has 2 pieces of approximations to raw leaf thereon.The nearly open and flat or upright stretching, extension of leaf, blade ovate, ovate
Lanceolar or ellipse, tool is a small number of sometimes or more and close aubergine spots above leaf.Two leaf pockets have very high gardening by orchid
It is worth with herbal medicine.Modes of reproduction is division propagation, and need every 3 years just can plant division it is primary, every clump will at least protect after plant division
5 soccer fraud stems to link together are deposited, the difficulty of this method is long period, satisfactory soccer fraud stem is few, difficulty is big,
Success rate is low.Conventional propagation method is difficult to meet the market demand, and tissue cultures are that two leaf pockets of quick breeding are most had by blue
Effect approach.Nineteen sixty, Morel propose the method for isolated vegetative propagation orchid.With the popularization of orchid, to orchid rapid propagation system
Research also gradually deeply.Various vegetative propagation techniques have become a hot topic of research.But due to the interspecific difference of biology, broad sense
On be applicable to the technology of orchid vegetative propagation, specific to two leaf pockets by might not be feasible on orchid, therefore, two leaf pockets be by blue
Quick study on reproduction is still a problem to be solved, and to protection Neottianthe plant wild resource and promotes to answer in gardens
With all having realistic meaning.
Chinese invention CN104920222A discloses the rapid propagation method of orchid, includes the following steps: that choosing orchid plants
Bud on the bulb of strain is scrubbed clean with dish washing liquid and is rushed with water as explant, after 40-50min, then surface sterilization, most
Afterwards with the 6 card aminoadenines for after aseptic water washing, putting MS+5.Omg/L, add the sucrose of 50g/L, adds the agar of 7g/L, PH to be
Culture induction seedling, condition of culture are as follows: 18-22 DEG C, intensity of illumination 1200-1400Lux, photoperiod be in 5.5 culture medium
Blade is cut to the blade of 0.9~1.1cm long with scissors when seedling grows to 2~3 leaves, leaf 6~7cm of length by 13h/d.
But foregoing invention can't be rapidly performed by breeding and can be reduced under the premise of guaranteeing the quality of survival rate and orchid
Cost and reduction pollution.
Summary of the invention
The technical problem to be solved in the present invention is that in view of the problems of the existing technology, two leaves pocket provided by the invention
It is the quick breeding by group culture method of explant by orchid bract, can be obtained in a short time using method of the invention a large amount of high-quality
Test tube seedling, solve the problems, such as that wild two leaves pocket is unable to large-scale production by orchid species and large-area applications exist.
To solve the above problems, provided by the invention quickly breed two leaf pockets by orchid by blue petal piece using two leaf pockets
Method includes the following steps:
(1) explant preparation step:
Two leaf pockets are chosen by the petal piece of blue plant as explant, scrubbed clean with dish washing liquid and pure steaming water is used to rinse
After 30min, aseptically, with 1.5% sodium hypochlorite surface sterilization 15min, then with 75% alcohol surface sterilization
30s, finally with spare after aseptic water washing；
(2) Fiber differentiation obtains adventitious bud step:
Using MS culture medium as minimal medium, then add 6-BA, concentration 0.3-0.5mg/L that concentration is 0.5-1.0mg/L
2,4-D, concentration be 0.5-2.0mg/L KT, concentration be 30g/L sucrose, concentration be 6g/L diatom powder and bacteriostatic agent, system
The induced medium for being 5.8 at PH, after the petal piece disinfection prepared in step (1), be placed in induced medium carry out it is indefinite
Bud inducement cultivation, condition of culture are as follows: temperature is at 17-23 DEG C, half-light culture 60-65 days, until obtaining adventitious bud；
(3) Multiplying culture adventitious bud step:
Using MS culture medium as minimal medium, 6-BA, concentration 1.0-3.0mg/L that addition concentration is 0.5-1.0mg/L
KT, the sucrose that concentration is 2, the 4-D of 0.2-0.4mg/L, concentration is 30g/L and the diatom powder that concentration is 6g/L and bacteriostatic agent,
The proliferated culture medium that PH is 5.8 is made, the adventitious bud cultivated in step (2) is placed in proliferated culture medium and carries out Multiplying culture,
The same step of condition of culture (2), until obtaining a large amount of adventitious buds；
(4) break up incubation step:
Using MS culture medium as minimal medium, add the 6-BA's and concentration 0.1-0.3mg/L of concentration 8.0-10.0mg/L
NAA, the sucrose of concentration 30g/L, the diatom powder and bacteriostatic agent that concentration is 6g/L, are made the differential medium that PH is 5.8, by step
(3) adventitious bud cultivated is placed in differential medium and cultivates, and condition of culture is: 17-23 DEG C, intensity of illumination be 1000-
1500Lux, photoperiod 15h/d, until sprout of the adventitious buds differentiation at a length of 3-4cm of leaf；
(5) culture of rootage step:
Using 1/2MS culture medium as minimal medium, 30g/L sucrose and 5g/L diatom powder, 2g active carbon, being prepared into PH is
The sprout cultivated in step (4) is taken single seedling to be placed in culture of rootage in root media, condition of culture by 5.8 root media
Same step (4), until sprout, which is taken root, obtains two leaf pockets by blue rooted seedling；
(6) hardening step:
When the seedling root long of step (5) culture arrives 1-3cm, by the sealed membrane of culture bottle opening, hardening 6-8 days；
(7) test tube seedling transplant step:
Test tube seedling transplanting medium is vinegar grain and vermiculite, carries out high-temperature sterilization before matrix transplanting, takes out the small of step (6) culture
The culture medium of root is eluted with water in seedling, slightly dries the moisture on surface, that is, in the matrix after being transplanted into disinfection, then builds arched shed,
Arched shed humidity is maintained at 20-25 DEG C of 85%-95%, temperature, transplanting gradually decreases temperature to after 17-20 DEG C, 40 days after 10 days
Into later period Cultivate administration.
Preferred technical solution, adding concentration in the step (2) again is the 6-BA of 0.75mg/L or 0.8mg/L, concentration
For 2, the 4-D of 0.4mg/L, concentration be 2.0mg/L KT, concentration be 30g/L sucrose, the concentration diatom powder that is 6g/L and antibacterial
Agent, the PH that induced medium is made is 5.8.
Preferred technical solution, the concentration of the middle addition 6-BA of the step (3) are that the concentration of 0.8mg/L, KT are 2.0mg/
L, the concentration of 2,4-D is 0.3mg/L, the concentration of sucrose is 30g/L and the weight percent 6g/L and bacteriostatic agent of diatom powder, wherein
The PH of proliferated culture medium is 5.8.
Preferred technical solution, the concentration 0.2mg/L of concentration 9.0mg/L, NAA of the middle addition 6-BA of the step (4),
The 30g/L of sucrose, the concentration 6g/L of diatom powder and bacteriostatic agent, the PH that differential medium is made is 5.8, condition of culture are as follows: 22 DEG C.
Preferred technical solution, vinegar grain in test tube seedling transplanting medium and vermiculite content in the step (7) are at least
80%, wherein vinegar grain and the weight ratio of vermiculite are 1:1 or 1.5:1.
Preferred technical solution, the test tube seedling transplanting medium further includes 10% or more mud stone and perlite, therein
Mud stone: the weight ratio of perlite is 3~5:1.5.
Preferred technical solution, the vermiculite Jin Hang Coarse-stone burn processing , Suo Shu Coarse-stone burn the temperature range of processing be 850 °~
880 °, Jin Hang Coarse-stone burning 15~after twenty minutes, it uses normal-temperature water or the water that vinegar is added as medium, is rapidly cooled to room temperature.
Preferred technical solution, the granularity of the vermiculite are 15mm~25mm, the mass percent of the vinegar and water
It is 5%.
Preferred technical solution, the later period Cultivate administration include carrying out dry and wet interval water supply pipe by blue seedling to two leaf pockets
Step is managed, the dry and wet interval moisturizing management process is included in exhausted water management in the range of 10 days~15 days, carries out exhausted water management
Next day afterwards, then supply water and pour, number interval holding 2 hours 1 time poured of supplying water amounts to and pours 3~4 times.
Preferred technical solution, the exhausted water management phase are 14 days.
It is provided by the invention using wild two leaves pocket by blue petal piece quickly breed two leaf pockets by blue method with it is existing
Technology is compared and is had the advantages that
(1) two leaf pocket carries out vegetative propagation using using tissue culture technique for the first time by blue, and breeding potential is mentioned than division propagation
It is high by 80%.
(2) in orchid tissue cultures it is Promethean using petal piece as explant evoking adventive bud test tube seedling,
Shorten one third in induced bud and rootage duration than generating protocorm using other explants.
(3) it replaces agar powder toxigenic capacity to save 2/3rds using diatom powder combination bacteriostatic agent, pollution rate can be made to reduce
90%.
(4) survival rate of the test tube seedling transplanting medium using vinegar grain+vermiculite than division propagation improves 90%.
Specific embodiment
Hereafter specific embodiments of the present invention are described in detail.
Embodiment 1
It is provided by the invention that two leaf pockets are quickly bred by blue method by blue petal piece using two leaf pockets, including walk as follows
It is rapid:
(1) explant preparation step:
Two leaf pockets are chosen by the petal piece of blue plant as explant, scrubbed clean with dish washing liquid and pure steaming water is used to rinse
After 30min, aseptically, with 1.5% sodium hypochlorite surface sterilization 15min, then with 75% alcohol surface sterilization
30s, finally with spare after aseptic water washing；The effect of the step is to prepare satisfactory aseptic explant.
(2) Fiber differentiation obtains adventitious bud step:
Using MS culture medium as minimal medium, then add 6-BA, concentration 0.3-0.5mg/L that concentration is 0.5-1.0mg/L
2,4-D, concentration be 0.5-2.0mg/L KT, concentration be 30g/L sucrose, concentration be 6g/L diatom powder and bacteriostatic agent, system
The induced medium for being 5.8 at PH, after the petal piece disinfection prepared in step (1), be placed in induced medium carry out it is indefinite
Bud inducement cultivation, condition of culture are as follows: temperature is at 17-23 DEG C, half-light culture 60-65 days, until obtaining adventitious bud；The present embodiment
In, it is preferred that add that the concentration of 6-BA is 0.75mg/L or 0.8mg/L, concentration are the 2 of 0.4mg/L in the step (2) again,
The diatom powder and bacteriostatic agent 0.07g that sucrose that KT that 4-D, concentration are 2.0mg/L, concentration are 30g/L, concentration are 6g/L, are made
The PH of induced medium is 5.8, which plays the role of being that induction generates adventitious bud, and the inductivity of adventitious bud is enable to reach
92%；
(3) Multiplying culture adventitious bud step:
Using MS culture medium as minimal medium, 6-BA, concentration 1.0-3.0mg/L that addition concentration is 0.5-1.0mg/L
KT, the sucrose that concentration is 2, the 4-D of 0.2-0.4mg/L, concentration is 30g/L and the diatom powder that concentration is 6g/L and bacteriostatic agent,
The proliferated culture medium that PH is 5.8 is made, the adventitious bud cultivated in step (2) is placed in proliferated culture medium and carries out Multiplying culture,
The same step of condition of culture (2), until obtaining a large amount of adventitious buds；Preferred numerical value is that the concentration of addition 6-BA is in the step (3)
The concentration of 0.8mg/L, KT are 2.0mg/L, the concentration of 2,4-D is 0.3mg/L, the concentration of sucrose is 30g/L and the weight of diatom powder
Percentage 6g/L and bacteriostatic agent 0.1g is measured, wherein the PH of proliferated culture medium is 5.8, and the effect of the step is to can be carried out adventitious bud increasing
Grow culture.
(4) break up incubation step:
Using MS culture medium as minimal medium, 6-BA and concentration 0.1-0.3mg/L that addition concentration is 8.0-10.0mg/L
NAA, the sucrose of concentration 30g/L, the diatom powder and bacteriostatic agent that concentration is 6g/L, the differential medium that PH is 5.8 is made, will walk
Suddenly the adventitious bud of (3) culture is placed in differential medium and cultivates, and condition of culture is: 17-23 DEG C, intensity of illumination be 1000-
1500Lux, photoperiod 15h/d, until sprout of the adventitious buds differentiation at a length of 3-4cm of leaf；Wherein, specific in the step (4)
Concentration values are as follows: 30g/L that add the concentration 0.2mg/L of concentration 9.0mg/L, NAA of 6-BA, sucrose, diatom powder it is dense
6g/L and bacteriostatic agent 0.1g is spent, the PH that differential medium is made is 5.8, condition of culture are as follows: 22 DEG C.
(5) culture of rootage step:
Using 1/2MS culture medium as minimal medium, 30g/L sucrose and 5g/L diatom powder, 2g active carbon, being prepared into PH is
The sprout cultivated in step (4) is taken single seedling to be placed in culture of rootage in root media, condition of culture by 5.8 root media
Same step (4), until sprout, which is taken root, obtains two leaf pockets by blue rooted seedling；
(6) hardening step:
When the seedling root long of step (5) culture arrives 1-3cm, by the sealed membrane of culture bottle opening, hardening 6-8 days, make test tube
Seedling can adapt to external environment as early as possible.
(7) test tube seedling transplant step:
Test tube seedling transplanting medium is vinegar grain and vermiculite, carries out high-temperature sterilization before matrix transplanting, takes out the small of step (6) culture
The culture medium of root is eluted with water in seedling, slightly dries the moisture on surface, that is, in the matrix after being transplanted into disinfection, then builds arched shed,
Arched shed humidity is maintained at 20-25 DEG C of 85%-95%, temperature, transplanting gradually decreases temperature to after 17-20 DEG C, 40 days after 10 days
Into later period Cultivate administration.Test tube seedling can grow young leaves under above-mentioned temperature and humidity conditions, be completed by photosynthesis independent raw
Growth process.
Embodiment 2
The present embodiment is further improved on the basis of embodiment 1, and difference is: provided by the invention to utilize two
Leaf pocket quickly breeds two leaf pockets by blue method by blue petal piece, vinegar grain in test tube seedling transplanting medium in step (7) and
Vermiculite content is that 80% or more, wherein vinegar grain and the weight ratio of vermiculite are 1:1 or 1.5:1.It can be with increased in the present embodiment
Technical solution, the test tube seedling transplanting medium further includes 10% or more mud stone and perlite, wherein preferred mud stone: perlite
Weight ratio be 3~5:1.5, guarantee that it is different by Multicomponent and cooperation for improving the supply of Different Nutrition ingredient
Gap, utilize the absorption of oxygen in air.
In the present embodiment, it is 850 °~880 ° that the vermiculite Jin Hang Coarse-stone, which burns processing , Suo Shu Coarse-stone and burns the temperature range of processing, into
Hang Coarse-stone burning 15~after twenty minutes, it uses normal-temperature water or the water that vinegar is added as medium, is rapidly cooled to room temperature, works as normal-temperature water
Shi , Coarse-stone is rapidly cooled as medium and burns that treated that vermiculite can not only expand, moreover it is possible to the effect sterilized.The food
The mass percent of vinegar and water is 5%, when using the water that vinegar is added as medium, can also obtain and be acidified to vermiculite surface,
So that the inorganic of vermiculite is salted out more, two leaf pockets is made to obtain sufficient nutrient by orchid.In the present embodiment, wherein of the vermiculite
Particle size range is 15mm~25mm, the preferably vermiculite of 15mm, and the vermiculite of this specification, more conducively two leaf pockets are carried out aerobic by orchid
Breathing carries out photosynthesis.
Embodiment 3
It is provided in this embodiment that two leaf pockets are quickly bred by blue method by blue petal piece using wild two leaves pocket, it is described
Later period Cultivate administration includes carrying out dry and wet interval moisturizing management process, the dry and wet interval moisturizing management by blue seedling to two leaf pockets
Step is included in exhausted water management in the range of 10 days~15 days, the next day after carrying out exhausted water management, then supply water and pour, and supplies
Pour number interval holding 2 hours 1 time of water amounts to 3~4 times.The present embodiment, the exhausted water management are selected as 14 days, are utilized
The management of exhausted water phase makes the root system of seedling improve the ability of being applicable in, and when water is more, root system carries out quickly absorbing moisture, in water shortage,
Root system is protected, and the root growth of seedling can be made more flourishing.
The preferred embodiments of the disclosure and embodiment are explained in detail above in conjunction with attached drawing, but it is of the invention
It is not limited to the above embodiment and embodiment can also not take off within the knowledge of those skilled in the art
It is made a variety of changes under the premise of from present inventive concept.
Claims (8)
1. quickly breeding two leaf pockets by blue method by blue petal piece using two leaf pockets, which comprises the steps of:
(1) explant preparation step:
Two leaf pockets are chosen by the petal piece of blue plant as explant, scrubbed clean with dish washing liquid and pure steaming water is used to rinse 30min
Afterwards, aseptically, with 1.5% sodium hypochlorite surface sterilization 15min, then with 75% alcohol surface sterilization 30s, finally
With spare after aseptic water washing；
(2) Fiber differentiation obtains adventitious bud step:
Using MS culture medium as minimal medium, then adding the concentration of 6-BA is the concentration 0.3- of 0.5-1.0mg/L, 2,4-D
The concentration of 0.5mg/L, KT are 0.5-2.0mg/L, the concentration of sucrose is 30g/L, the concentration of diatom powder is 6g/L and bacteriostatic agent, system
The induced medium for being 5.8 at PH, after the petal piece disinfection prepared in step (1), be placed in induced medium carry out it is indefinite
Bud inducement cultivation, condition of culture are as follows: temperature is at 17-23 DEG C, half-light culture 60-65 days, until obtaining adventitious bud；
(3) Multiplying culture adventitious bud step:
Using MS culture medium as minimal medium, add 6-BA concentration be 0.5-1.0mg/L, KT concentration be 1.0-3.0mg/L,
The diatom powder and bacteriostatic agent that the sucrose and concentration that 2,4-D concentration is 0.2-0.4mg/L, concentration is 30g/L are 6g/L, are made PH
For 5.8 proliferated culture medium, the adventitious bud cultivated in step (2) is placed in proliferated culture medium and carries out Multiplying culture, cultivates item
The same step of part (2), until obtaining a large amount of adventitious buds；
(4) break up incubation step:
Using MS culture medium as minimal medium, the NAA of the 6-BA and concentration 0.1-0.3mg/L of addition concentration 8.0-10.0mg/L,
The sucrose of concentration 30g/L, the diatom powder and bacteriostatic agent that concentration is 6g/L, are made the differential medium that PH is 5.8, by step (3)
The adventitious bud of culture, which is placed in differential medium, to be cultivated, and condition of culture is: 17-23 DEG C, intensity of illumination be 1000-1500Lux,
Photoperiod is 15h/d, until sprout of the adventitious buds differentiation at a length of 3-4cm of leaf；
(5) culture of rootage step:
Using 1/2MS culture medium as minimal medium, 30g/L sucrose and 5g/L diatom powder, 2g active carbon, being prepared into PH is 5.8
The sprout cultivated in step (4) is taken single seedling to be placed in culture of rootage in root media, the same step of condition of culture by root media
(4), until sprout, which is taken root, obtains two leaf pockets by blue rooted seedling；
(6) hardening step:
When the seedling root long of step (5) culture arrives 1-3cm, by the sealed membrane of culture bottle opening, hardening 6-8 days；
(7) test tube seedling transplant step:
Test tube seedling transplanting medium is vinegar grain and vermiculite, carries out high-temperature sterilization before matrix transplanting, and the seedling for taking out step (6) culture is used
Water cleans the culture medium of root, slightly dries the moisture on surface, that is, in the matrix after being transplanted into disinfection, then builds arched shed, will encircle
Canopy humidity is maintained at 20-25 DEG C of 85%-95%, temperature, and transplanting gradually decreases temperature to entering after 17-20 DEG C, 40 days after 10 days
Later period Cultivate administration.
2. quickly breeding two leaf pockets by blue method, feature by blue petal piece using two leaf pockets according to claim 1
It is, the concentration for adding 6-BA in the step (2) again is 0.75mg/L or 0.8mg/L, the concentration of 2,4-D is 0.4mg/L, KT
Concentration be 2.0mg/L, the concentration of sucrose is 30g/L, the concentration of diatom powder is 6g/L and bacteriostatic agent, induced medium is made
PH is 5.8.
3. quickly breeding two leaf pockets by blue method, feature by blue petal piece using two leaf pockets according to claim 1
Be, in the step (3) concentration of addition 6-BA be the concentration of 0.8mg/L, KT be 2.0mg/L, the concentration of 2,4-D is
0.3mg/L, sucrose concentration be the weight percent 6g/L and bacteriostatic agent of 30g/L and diatom powder, the wherein PH of proliferated culture medium
It is 5.8.
4. quickly breeding two leaf pockets by blue method, feature by blue petal piece using two leaf pockets according to claim 1
It is, the concentration 0.2mg/L of concentration 9.0mg/L, NAA of addition 6-BA, the 30g/L of sucrose, diatom powder in the step (4)
Concentration 6g/L and bacteriostatic agent, be made differential medium PH be 5.8, condition of culture are as follows: 22 DEG C.
5. two leaf pockets are quickly bred by blue method by blue petal piece using wild two leaves pocket according to claim 1,
It being characterized in that, it is 850 °~880 ° that the vermiculite Jin Hang Coarse-stone burning processing , Suo Shu Coarse-stone, which burns the temperature range of processing, Jin Hang Coarse-stone burning 15~
After twenty minutes, it uses normal-temperature water or the water that vinegar is added as medium, is rapidly cooled to room temperature.
6. two leaf pockets are quickly bred by blue method by blue petal piece using wild two leaves pocket according to claim 5,
It is characterized in that, the granularity of the vermiculite is 15mm~25mm, and the mass percent of the vinegar and water is 5%.
7. two leaf pockets are quickly bred by blue method by blue petal piece using wild two leaves pocket according to claim 1,
It is characterized in that, the later period Cultivate administration includes carrying out dry and wet interval moisturizing management process by blue seedling to two leaf pockets, described dry
Wet interval moisturizing management process, is included in exhausted water management in the range of 10 days~15 days, the next day after carrying out exhausted water management, then into
Row supplies water and pours, number interval holding 2 hours 1 time poured of supplying water, and amounts to 3~4 times.
8. quickly breeding two leaf pockets by blue method, feature by blue petal piece using two leaf pockets according to claim 7
It is, the exhausted water management is 14 days.
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